The utility model relates to the technical field of glass substrate production, and discloses a glass substrate spacing paper screening device which comprises an experiment A-shaped frame simulating the design of a normal packaging A-shaped frame, the experiment A-shaped frame comprises a supporting back plate, and a detection part used for assisting in judging whether spacing paper meets the standard or not is further arranged in the extending direction of the supporting back plate. A user judges whether the spacing paper meets the requirement or not based on the position of the part, exceeding the glass substrate, of the spacing paper on the detection component. According to the utility model, the judgment is more visual, judgment errors caused by subjective factors are reduced, and the judgment accuracy is improved.