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261results about How to "Shorten screening time" patented technology
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Fish breeding method for improving CRISPR-Cas9 gene editing and passage efficiency through fish roe preserving fluid
ActiveCN106191124AHigh target shooting efficiencyImproving gene editing passaging efficiencyMicroinjection basedAnimal husbandryFisheryMicroinjection
The invention belongs to the field of fish molecular breeding, and particularly relates to a fish breeding method for improving CRISPR-Cas9 gene editing and passage efficiency through fish roe preserving fluid. According to the method, the fish roe preserving fluid technology, the microinjection technology an the CRISPR-Cas9 gene editing technology are ingeniously combined, the targeting efficiency of the CRISPR-Cas9 gene editing technology and gene editing passage efficiency are greatly improved, the screening time needed by a gene editing method for fish breeding is obviously saved, and the method is of great significance in promoting rapid development of gene editing fish breeding.
Owner:CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI
Screening method of salt resisting germplasms of corn
The invention relates to a screening method of salt resisting germplasms of corns. The method comprises the following steps: after seeds are disinfected, soaking the seeds in saline liquor with different concentrations for 12 hours for a germinating and seedling growth experiment; in a stress experiment in a germinating stage, supplementing 15ml of saline liquor for one time every day; after the seeds are germinated for 4 days, retaining the germinating seeds which are consistent in growth for each sample, and determining the content of Na+ and K+ ions; in the seedling growth experiment, directly seeding the corn seeds disinfected and soaked in disinfected vermiculites containing 0mmol / L, 60mmol / L, 140mmol / L and 220mmol / L NaCl liquids; placing the seeds in a greenhouse for stress treatment; and determining the content of Na+ and K+ ions when the controlled seedling has three leaves and one heart. According to the method provided by the invention, the salt resisting germplasms are screened through the Na+ and K+ ions and K+ / Na+ ratio in parts of different selfing lines in the germinating stage and seedling growth stage, so that the screening workload is effectively reduced, the screening time is shortened, and the screening efficiency and the accuracy of the result are improved.
Owner:安徽英诺管理咨询有限公司
Large traffic searching method and system
ActiveCN103412934AShorten screening timeSave time on self-screeningSpecial data processing applicationsScreen timeReal-time computing
The invention provides a large traffic searching method. The large traffic searching method comprises the following steps: inputting a departure place, a destination and departure time by a user; receiving data input by the user and processing; judging whether a through route and a transit route exist between the departure place and the destination or not; feeding information of the through route and the transit route back to the user; when the through route and the transit route do not exist between the departure place and the destination, feeding information of inexistence of the through route and transit route back to the user. The invention further provides a large traffic searching system. The large traffic searching method and system provided by the invention have the advantages that comprehensive transportation inquiry is provided based on various vehicles including flights, trains, buses and the like; finally, a specific route plan is determined. With the adoption of the method, the user can obtain a suggested route only by inputting the departure place, the destination and the departure time, the method aims to help the user to select the most suitable tripping route, and screening time of the user is reduced.
Owner:HEFEI HANGLIAN CULTURE COMM CO LTD
Superfluous picture deletion method and device
ActiveCN103970516AResolution timeResolve accuracySpecial data processing applicationsSpecific program execution arrangementsComputer engineering
The invention discloses a superfluous picture deletion method and a superfluous picture deletion device, and belongs to the field of computers. The deletion method comprises the followings steps of acquiring the marks of all pictures in a program installation package; extracting picture relevant files in the program installation package, which are files relevant with applied pictures in the program installation package; acquiring the marks of the applied pictures in the program installation package according to the extracted picture relevant files; acquiring the marks of superfluous pictures on the basis of the marks of all pictures and the marks of the applied pictures; and deleting the superfluous pictures in the program installation package according to the marks of the superfluous pictures. According to the superfluous picture deletion method and the superfluous picture deletion device, disclosed by the invention, the marks of all the pictures and the marks of the applied pictures in the program installation package are obtained, the superfluous pictures can be deleted according to the marks of all the pictures and the marks of the applied pictures, and the purposes of shortening the selection time of the superfluous pictures and improving the selection accuracy of the superfluous pictures can be achieved.
Owner:SHENZHEN TENCENT COMP SYST CO LTD
Method and system for determining optimal intra-frame prediction mode
ActiveCN106231302ALow rate distortion penaltyShorten screening timeDigital video signal modificationIntra-frameRate distortion
The invention discloses a method and a system for determining an optimal intra-frame prediction mode. The method for determining the optimal intra-frame prediction mode comprises the following steps: determining a main sampling direction of a current PU (Prediction Unit) according to texture information of the current PU or an optimal intra-frame prediction mode of an upper layer PU; if the main sampling direction of the current PU is determined, performing sparse and dense sampling on an angle mode of the current PU according to a variable sampling step length strategy to obtain a sampling angle mode; if the main sampling direction of the current PU is not determined, performing uniform sampling on the angle mode of the current PU according to a uniform sampling step length strategy to obtain a sampling angle mode; processing the sampling angle mode to obtain a candidate angle mode; and constructing a candidate intra-frame prediction mode set including the candidate angle mode, and determining an intra-frame prediction mode with a minimum rate-distortion cost from the candidate intra-frame prediction mode set as the optimal intra-frame prediction mode. Through the technical scheme, the optimal intra-frame prediction mode of the current PU can be determined rapidly and accurately.
Owner:高前文
Method for determining highly accelerated stress screening test condition
InactiveCN102193054AShorten screening timeShorten the production cycleElectronic circuit testingNon-linear opticsScreening testEnvironmental geology
The invention provides a method for determining a highly accelerated stress screening test condition. The method comprises the following steps of: firstly, setting an initial testing condition according to the temperature and vibration comprehensive stress operation and damage limit of a product to carry out temperature and vibration comprehensive stress test; accurately obtaining the highly accelerated stress screening test condition by using a successive approximation method; and then verifying by using a typical defect of the product to obtain the validity of the screening test condition; and finally, obtaining the effective highly accelerated stress screening test condition of the temperature and vibration comprehensive stress through repeated tests and scientific adjustment. By using the test condition obtained by using the method provided by the invention, the screening test time can be largely reduced, the testing efficiency can be increased and the test cost can be saved.
Owner:SHANGHAI MICRO ELECTRONICS EQUIP (GRP) CO LTD
Resonant frequency tracking circuit
ActiveCN103414463AShorten the timeReduce manufacturing costPulse automatic controlLc resonant circuitPhase locked loop circuit
The invention discloses a resonant frequency tracking circuit. The resonant frequency tracking circuit comprises a current switch for converting direct voltage inputted by a power supply into a high-frequency pulse current; an LC resonant circuit connected to an output end of the current switch and used for converting electric energy into electromagnetic energy and then transmitting the electromagnetic energy to a wireless charger; a detection inductor connected to the ground at an end and used for obtaining frequency and phase information of the LC resonant circuit; and a phase locked loop circuit connected to the other end of the detection inductor and used for correcting the frequency and phase information and then outputting the frequency and phase information to the current switch as a feedback signal so as to control pulse current frequency outputted by the current switch. According to the invention, the inductive wireless charger LC resonant frequency automatic tracking device reduces the required accuracy of LC parameters from 1% to 10%, and therefore production costs are reduced, device screening time is saved and production efficiency is improved.
Owner:CRM ICBG (WUXI) CO LTD
Data deletion method and mobile terminal thereof
ActiveCN103383698AShorten screening timeImprove efficiency and accuracySpecial data processing applicationsRedundant operation error correctionBackupLog data
The invention is applicable to the technical field of communication, and provides a data deletion method and a mobile terminal thereof. The method comprises the steps as follows: recording step: operation information about data backup is recorded; prompting step: when performing data deletion, a user is prompted to delete the backed-up data via the operation information. Therefore, data deletion accuracy and efficiency are improved.
Owner:YULONG COMPUTER TELECOMM SCI (SHENZHEN) CO LTD
Compound having alpha-glucosidase inhibitory activity in lotus leaves and application
InactiveCN102827221AReduce blindnessImprove screening efficiencySugar derivativesMetabolism disorderAlglucerasePrimary screening
The invention provides a compound having an alpha-glucosidase inhibitory activity in lotus leaves. Nine compounds having the alpha-glucosidase inhibitory activity are screened from the lotus leaves through magnetic bead separation and liquid chromatogram-mass spectrometry analysis, and the obtained compounds are confirmed to have the alpha-glucosidase inhibitory activity through a verification of the alpha-glucosidase inhibitory activity. Compared with tradition methods, separated compounds are not needed to prepare during a primary screening, thereby substantially improving screening efficiency and shortening screening time. Due to changing a traditional activity-screening mode which separates firstly and screens secondly to a novel activity-screening mode which screens firstly and separates secondly, blindness of preparing and separating compounds is greatly reduced. The method can be popularized and applied to quick screening of compounds having the alpha-glucosidase inhibitory activity from natural medicines or herbal mixtures. The obtained compounds can be used for preparing anti-diabetic drugs.
Owner:ZHEJIANG UNIV
Drug-target interaction prediction model method based on deep embedding learning of molecular graph and sequence
PendingCN113327644AImprove predictive performanceShorten screening timeNeural architecturesNeural learning methodsGraph neural networksDrug target
The invention provides a drug-target interaction prediction model method based on deep embedding learning of a molecular graph and a sequence, and the method comprises the steps: building a map neural network based on an attention mechanism and an attention-oriented bidirectional LSTM to predict the interaction, wherein in order to achieve the more effective training, a pre-training model BERT is utilized to extract embedded vector representation of each sub-sequence from a protein sequence, and meanwhile, a local breadth-first search algorithm is designed to extract sub-graph information of a drug molecular graph, so that a graph neural network learns higher feature information. On one hand, in the aspect of drug molecules, better spatial features can be learned based on the molecular graph; on the other hand, the protein sequence data size is large, larger protein space can be covered, and the generalization ability is improved.
Owner:SUN YAT SEN UNIV
Method and device for screening text content
ActiveCN104850537AImplement automatic filteringEasy to operateSpecial data processing applicationsFeature vectorData source
Owner:TENCENT TECH (SHENZHEN) CO LTD +1
Screening method for deep-seeding-resisting corn germplasms
ActiveCN103238397AReduce workloadShorten screening timeSeed and root treatmentCultivating equipmentsBiologyWorkload
The invention relates to a screening method for deep-seeding-resisting corn germplasms. The method comprises the following steps: collecting corn germplasm materials in areas; selecting 90 full and unbroken seeds from the corn germplasm materials; determining an experimental barrel which is 25cm in height and 16cm in diameter and corresponds to each variety, packaging vermiculites which are 10cm deep uniformly to all experimental barrels before seeding, and thoroughly pouring water; then, uniformly seeding 30 full and unbroken seeds selected from the selfing lines to vermiculites in the experimental barrels, then covering the vermiculites by 15cm, and repeating the experiment for 3 times; after seeding the germplasm materials, placing the germplasm materials in a same heliogreenhouse, maintaining the indoor temperature at 25+ / -2 DEG C and humidity at 70%+ / -3, and naturally germinating to emerge; in 10 days, carrying out statistic on rate of emergence, and retaining 10 strains which are consistent in growth for each sample to determine root, root length, mesocotyl length, coleoptile length, seedling length and the like; identifying and evaluating the deep-seeding resistances for the germplasms and screening the self lines with strong deep-seeding resistances. According to the method provided by the invention, the screening workload is reduced, and the screening efficiency and the accuracy of the screening result are improved.
Owner:安徽英诺管理咨询有限公司
Data screening method and device, and securitization loan screening method and device
ActiveCN101221578AReduce process overheadShorten screening timeFinanceSpecial data processing applicationsDistribution controlData records
The invention discloses a method for data sieving and is applied in the sieving of the data records in a database at least comprising a first data item, a second data item and a third data item. The invention comprises that: the pre-sieving is executed, the data records which can not meet the sieving requirement are get rid of to form a preselection base; the data records which meet the sieving conditions are sieved from the preselection base to form a candidate base; the content of the first data item used for random distribution control and the content of the second data item used for concentration degree control are extracted; the random distribution control is executed according to the content of the first data item, a data record is randomly sieved, then the concentration degree control is made to the data record according to the content of the second data item; if the record meets the requirement of the concentration degree, whether the content of the third data item of the data record meets the matching conditions is judged, if the content of the third data item of the data record meets the matching conditions, the data record is moved into a target database. The invention also provides a data sieving device as well as a sieving method and a sieving device for securitization loan. The invention has less time consumption and higher efficiency during the data sieving.
Owner:CHINA CONSTRUCTION BANK
Oil-degradation oil-and-salt tolerance bacterial strain, and screening method and application thereof
ActiveCN103205378AGood oil and salt resistanceFast growthBacteriaContaminated soil reclamationEscherichia coliScreening method
The invention relates to an oil-degradation oil-and-salt tolerance bacterial strain which is named as SY-1. The systematic name of the SY-1 is Escherichia coli, the file number is CGMCC (China General Microbiological Culture Collection Center No.7075, the file date is December 31, 2012, the file address is the Micro-organism Study of Chinese Academy of Sciences, No.3, No.1 Beichen west road, Chaoyang district, Beijing city, and the file unit is China General Microbiological Culture Collection Center. The oil-degradation oil-and-salt tolerance bacterial strain is good in oil and salt tolerance, is Gram-negative bacterium shaped in short rhabditiform and with flagellum and no spores and active, is in citrate and glucose ammonium salt positive property and can generate catalase without gelatinase. The bacterial strain is fast in growth, can grow in oil used as unique carbon source, and has the advantages of oil degradation.
Owner:航天圣诺(北京)环保科技有限公司
User monitoring method and device
ActiveCN103888961AEasy to implementEasy to operateWireless communicationProblem of timeResidence time
The embodiments of the invention disclose a user monitoring method and device. The method comprises the following steps: when the end time of the current update period arrives, a default algorithm is adopted to calculate the residence time of each user in a default region in network data recorded by base stations; users of which the residence time in the default region in the current update period is greater than a first threshold value are classified as a first kind of users and stored into a list of the first kind of users; users of which the residence time in the default region in the current update period is less than a second threshold value are classified as a second kind of users and stored into a list of the second kind of users; and other users except users recorded in the list of the first kind of users and the list of the second kind of users corresponding to the current update period are screened out from all users recorded in the network data, and the other users are monitored. The method and device of the invention are used to solve the problem of time and resource waste caused by identification device installation and utilization and identification chip distribution and recovery in the prior art.
Owner:XINYANG BRANCH HENAN CO LTD OF CHINA MOBILE COMM CORP
Application method of serum lipid biomarker in NSCLC early diagnosis
ActiveCN108680745AShorten screening timeHigh precisionComponent separationOmicsMetaboliteLipid biomarker
An application method of a serum lipid biomarker in NSCLC early diagnosis includes the following steps: collecting serum samples of NSCLC patients, patients with benign lung lesions and normal people;pretreating the serum samples, and detecting lipid metabolism markers in each serum sample by utilizing a method of ultra-high performance liquid chromatography-quadrupole-time of flight mass spectrometry; screening NSCLC-related differential lipid metabolism markers through a method of multivariate pattern recognition analysis; screening out key metabolic pathways with highest correlation with the lipid metabolites through KEGG analysis and metabolic pathway analysis of NSCLC differential lipid metabolites; performing network analysis of "gene-enzyme-reaction-metabolite" of the NSCLC differential lipid metabolites, to obtain a network graph of the NSCLC differential lipid metabolites; and screening and obtaining the serum lipid biomarker for the NSCLC early diagnosis through integrationof the screening of the NSCLC differential lipid metabolism markers and analysis results of the metabolic pathways.
Owner:HUZHOU CENT HOSPITAL
Lightweight block cipher algorithm Shadow implementation method and device and computer readable medium
ActiveCN110572255AIncrease confusionEffective against attackEncryption apparatus with shift registers/memoriesDigital data protectionComputer hardwareS-box
The invention discloses a lightweight block cipher algorithm Shadow implementation method and device based on an SPN structure and a computer readable medium. The method comprises the following steps:obtaining a 64-bit plaintext to be encrypted, and determining an iteration round number R according to the number of key bits; in the first round of operation to the Rth round of operation, enablingeach round of operation to be sequentially subjected to round key addition operation, nonlinear S-box replacement operation, row displacement operation and linear column hybrid operation, and updatinga key; taking the data obtained after the linear column hybrid operation as the to-be-encrypted data of the next round of operation, and taking the updated key as the key of the next round of operation; and performing round key addition operation on the data after the Rth round of operation and the key, and outputting a ciphertext. On the basis that part of cryptographic indexes of the S box arenot affected by affine transformation, a composite affine mathematical method is adopted, the pseudo-random key related S box is constructed on a finite field GF (24), meanwhile, a column hybrid matrix is constructed according to the Schmidt matrix orthogonalization principle, and attacks can be effectively resisted.
Owner:HENGYANG NORMAL UNIV
Four sets of oligonucleotide sequences for recognition and identification of vibrio anguillarum, and screening method of oligonucleotide sequences
PendingCN110578010AReduce process linksShorten the number of screening roundsMicrobiological testing/measurementMicroorganism based processesVibrio anguillarumScreening method
The invention discloses four sets of oligonucleotide sequences for recognition and identification of vibrio anguillarum, and a screening method of the oligonucleotide sequences, and relates to the technical field of recognition detection of vibrio anguillarum. Two ends of each oligonucleotide sequence are fixed sequences, intermediate sequences are arrayed according to the order of 5'-3' as follows: a first set contains two sequences of H1 and H2, a second set contains five sequences of H5, H6, H26, H38 and H33, a third set contains three sequences of H12, H25 and H42, and a fourth set contains a sequence of H28; and any of the oligonucleotide sequences in each set serves as the intermediate sequence of an aptamer to be used for vibrio anguillarum detection. The screening method of the sequences sequentially comprises the steps of random oligonucleotide library synthesis, combination, separation, PCR amplification and high-throughput sequencing which are circulated for 3-5 rounds, andfinally, the target sequences are obtained. The sequence length of the aptamer of the vibrio anguillarum is shortened, and the screening method is quick.
Owner:JIMEI UNIV
High-content rapid screening method for magnetic beads of ocean natural active materials
ActiveCN103675115ALarge amount of informationGood reproducibilityComponent separationAnalysis using nuclear magnetic resonanceScreening durationMagnetic bead
The invention relates to the technical field of screening of natural medicines and provides a high-content rapid screening method for magnetic beads of ocean natural active materials. The method is a screening and evaluating method for active materials based on magnetic bead bonds and high-content multi-target protein integration. According to the high-content rapid screening method, the configuration is as follows: a hardware system is composed of a permanent magnet, the magnetic beads and targets; a software system is responsible for acquiring hygroplasm data. Compared with a conventional ultraviolet screening method, the screening method has the advantages that the screening efficiency is obviously improved and the screening time is shortened; various biological activities can be investigated at the same time and the synergistic effect among compounds is found. The screening method is rapid, accurate, great in information amount and good in repeatability and can be used for screening a natural product library or a combinatorial chemistry library in a large scale.
Owner:张文 +2
Method for screening protein aptamers by using micro-fluidic chip
InactiveCN102321616AReduce adsorptionIncrease screening strengthDNA preparationAptamerProtein target
The invention discloses a method for screening protein aptamers by using a micro-fluidic chip. The method comprises steps that: a preset initial nucleic acid library is first incubated with a non-target protein, such that an optimized nucleic acid library is obtained; a target protein is incubated with microspheres, the target-protein-modified microspheres are positioned in a channel of a micro-fluidic chip; the optimized nucleic acid library is delivered into the channel of the micro-fluidic chip; the materials are processed through washing and eluting, such that an initial screened nucleic acid library is obtained; PCR amplification is carried out upon the initial screened nucleic acid library, the amplification products are separated through an affinity column, and the products are processed from denaturative melting and desalting, such that a primary nucleic acid library is obtained; the primary nucleic acid library is used for replacing the initial nucleic acid library, and the previous steps are repeated until a nucleic acid library containing target aptamers is obtained. The method provided by the invention has advantages of good versatility, short screening time, high screening efficiency, and good screening effect.
Owner:HUNAN UNIV
Information recommendation method and apparatus
InactiveCN109388756ASatisfy taste budsShorten screening timeDigital data information retrievalBuying/selling/leasing transactionsUser needsComputer science
The invention discloses an information recommendation method and a device. The method comprises the following steps: obtaining the current position information of the user and the user identification;Determining a user interest object having an interest relationship with the user identity according to the user identity; Searching for dish recommendation information and / or store recommendation information of a user-focused object matching the user's current location information; Recommending the dish recommendation information and / or store recommendation information to the user. Based on the scheme provided by the invention, Recommended dishes and / or shops to the user, meet the taste bud needs of users, so that users can taste delicious dishes, No user-to-user screening required, The invention saves the user screening time, enables the user to eat quickly, improves the user experience, and also overcomes the problems that the existing recommendation based on the user history order record or the browsing record leads to the single recommended dishes and / or the store types, the reference value is low, the user needs to screen the types, and the user taste buds can not be satisfied.
Owner:ZHEJIANG KOUBEI NETWORK TECH CO LTD
Technological parameter determining method of metal material 3D printing
ActiveCN106735205AShorten screening timeReduce consumptionAdditive manufacturing apparatusIncreasing energy efficiencyMultiple pointHardness
The invention discloses a technological parameter determining method of metal material 3D printing. The method comprises the steps that range values of the powder layer thickness, laser power and scanning speed parameters are determined; multiple point values are arranged within all parameter ranges in an arithmetic value progression mode; all the point values are in one-to-one correspondence to form multiple I type parameter groups, and metal sample blocks are printed for all the I type parameter groups; the obtained sample blocks are subjected to a hardness test, a I type parameter group value with the highest hardness is selected, upper and lower point values of laser power point values and scanning speed point values in the I type parameter group are determined, and the obtained point values are in one-to-one correspondence to form nine II type parameter groups; tensile test bars are printed for all the II type parameter groups; the obtained tensile test bars are subjected to a tensile test, a II type parameter group value with the best mechanical property is obtained, and then the technical parameter suitable for metal material 3D printing is obtained. Accordingly, by means of a regular parameter combination mode, the parameter range is narrowed through the hardness test, the most suitable parameter is obtained through the tensile test, and the method is simple, reliable, economical and practical.
Owner:TSC LASER TECH DEV BEIJING CO LTD
Kit for aptamer screening and use and detection method thereof
PendingCN110029110AImprove performanceImprove stabilityMicrobiological testing/measurementDNA preparationBiotin-streptavidin complexMagnetic bead
The invention discloses a kit for aptamer screening. The kit comprises the following raw materials: a magnet, streptavidin-modified magnetic beads, a nucleic acid library, a short sequence, a PCR liquid premix, ePCR micro-droplet generation oil, a fluorescence quantitative PCR liquid premix and a molecular weight standard sample. The invention also discloses a using method of the kit for aptamer screening. The method comprises the following steps: S1, immobilizing the nucleic acid library on the magnetic beads; S2, performing screening; S3, performing PCR amplification; S4, preparing ssDNA; and S5, performing multi-round screening. The invention also discloses a method for detecting the binding rate of aptamer screened by a magnetic bead method and a target. According to the invention, thescreening period is shortened, the screening time is saved, the experimental flow is simplified, the repeatability is good, and the screening efficiency is high.
Owner:安徽省昂普拓迈生物科技有限责任公司
Microbial flora continuous screening device
PendingCN107384758APromote degradationImprove living conditionsBioreactor/fermenter combinationsBiological substance pretreatmentsMicroorganismMetabolite
The invention discloses a microbial flora continuous screening device which comprises a culture medium storer, a feed liquid reaction chamber and a microbial flora collecting chamber. The bottom of the culture medium storer is connected to the feed liquid reaction chamber through a first hose, the bottom of the feed liquid reaction chamber is connected to the microbial flora collecting chamber through a second hose, and a drain hose is arranged at the bottom of the microbial flora collecting chamber. Based on a microbial continuo's culture theory, microbial screening is located in a dynamic state, nutritional substances required by microorganisms can be immediately and continuously supplemented, and part of harmful metabolites can be immediately discharged to promote growth and reproduction of microorganisms; by taking nutritional substances such as the culture medium as a food of microorganisms, related microbial flora is promoted to form a food chain, and finally, target degrading flora is quickly screened, so that the demand on screening the microbial mixed flora in industrial scaled production is met.
Owner:INNER MONGOLIA UNIV OF SCI & TECH
Hotel sequencing method based on traffic convenience degree in OTA (online travel agent) website
InactiveCN106909659AQuick selectionEasy retrievalReservationsBuying/selling/leasing transactionsGeographic siteLower score
Owner:CTRIP TRAVEL NETWORK TECH SHANGHAI0
Method for fast screening mammal cell strain having high exogenous protein expression level
ActiveCN104087612AQuick filterEfficient screeningVector-based foreign material introductionForeign genetic material cellsScreening methodCell strain
The invention relates to a method for fast screening a mammal cell strain having a high exogenous protein expression level. The method utilizes a novel high-expression carrier, realizes efficient expression of a desired protein in an appropriate mammal host cell, and realizes fast screening of a cell strain having a high desired protein expression level by a unique screening method. The method greatly reduces time and a cost of screening of the cell strain having a high desired protein expression level and substantially improves an expression level of the exogenous protein in the mammal host cell.
Owner:UNICOHEALTH CO LTD
Method for screening hybridoma cells secreting specific monoclonal antibodies, and application thereof
ActiveCN104977408AShorten screening timeReduce lossBiological testingMethyl celluloseFluorescence microscope
The invention discloses a method for screening hybridoma cells secreting specific monoclonal antibodies, and an application thereof. The method comprises the following steps: coupling Oleyl-PEG4000-NHS with Ag to obtain Oleyl-PEG4000-NHS-Ag; adding Oleyl-PEG4000-NHS-Ag in cloning of hybridoma cells to be screened, culturing, removing the above obtained supernatant, and cleaning; adding a fluorescent substance-labeled anti-mouse second antibody, incubating, removing the obtained supernatant, and cleaning; and adding an incomplete medium, observing cell masses under visible light and fluorescence by using an inverted fluorescence microscope, carrying out negative and positive labeling, adding a methyl cellulose semisolid medium to plate holes containing positive cell masses, transferring positively labeled cells to a new cell culture plate, and culturing to obtain the hybridoma cells secreting the specific monoclonal antibodies. The method has the advantages of rapidness, simplicity and high accuracy, and can be used for rapid screening to obtain the hybridoma cells secreting the specific monoclonal antibodies.
Owner:广东忠信生物科技有限公司
Question processing method and device based on semantic matching, terminal and storage medium
PendingCN112632257AImprove processing efficiencyImprove accuracySemantic analysisCharacter and pattern recognitionScreening durationSemantic matching
The embodiment of the invention discloses a question processing method and device based on semantic matching, a terminal and a storage medium, and the method comprises the steps of obtaining a to-be-analyzed question, processing the to-be-analyzed question, obtaining a label of the to-be-analyzed question, obtaining a standard question set corresponding to the label, determining semantic similarity between the to-be-analyzed questions and each standard question in the standard question set, screening out target standard questions from the standard question set based on the semantic similarity, determining answers corresponding to the to-be-analyzed questions based on target answers corresponding to the target standard questions, and displaying the answers corresponding to the to-be-analyzed questions. By implementing the method, the labels of the questions can be analyzed, and the answers to the questions are found based on the labels of the questions, so that the screening time of the answers to the questions is shortened, and the processing efficiency and processing accuracy of the questions are improved. The scheme is applied to a medical scene, for example, questions raised by a patient are intelligently answered, and digital medical treatment is realized.
Owner:深圳赛安特技术服务有限公司
Grain drying and screening integrated device
The invention discloses a grain drying and screening integrated device. The grain drying and screening integrated device comprises a case, a spreading device, an air heater, a screening box and an impurity box. The spreading device is arranged in the case. Two horizontal sliding rods are arranged in the case. The two ends of each sliding rod are welded to the inner wall of the case. The screeningbox is slidably mounted between the two sliding rods. A first screen, a second screen and a third screen are sequentially arranged in the screening box from top to bottom. Compared with an ordinary spreading device, a flat spreading plate is indirectly driven by a driving motor to move left and right so that grain can be evenly spread on a conveying belt, the situation that the grain is accumulated in the feeding process, and consequentially, the drying effect is affected is prevented; the screening box moves left and right in the screening process, so that the flow rate of the grain in the screening box is improved, and the screening speed is improved; and meanwhile, a first screen, a second screen and a third screen are arranged in the screening box, so that grain with various sizes is separated, secondary operation is not needed, and the screening time is shortened.
Owner:ANHUI HUARUI PHARMA TECH DEV CO LTD
Fibrinolytic enzyme-producing Bacillus subtilis and fermentation method and application thereof
ActiveCN105238720AImprove securitySimple recipeBacteriaPeptide/protein ingredientsMicrobiologyBacillus subtilis
The invention discloses a fibrinolytic enzyme-producing Bacillus subtilis and a fermentation method and application thereof. The Bacillus subtilis is isolated and sieved from a traditional Chongqing fermented food, i.e., fermented soya beans. The Bacillus subtilis DC27 is collected in China Center for Type Culture Collection under CCTCC NO: M 2015573. The invention further provides a liquid fermentation method of the Bacillus subtilis, and the fibrinolytic enzyme in obtained fermentation broth has an activity of up to 4506 IU / ml. The Bacillus subtilis is from foods and can secrete the high-activity fibrinolytic enzyme; the Bacillus subtilis is safe and nontoxic, and is applicable to the development of functional foods or drugs for preventing or relieving thrombotic diseases; and the Bacillus subtilis has a promising application prospect.
Owner:HUAZHONG AGRI UNIV
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