The invention discloses a method for improving
cell surface display efficiency and a product, and belongs to the technical field of
biology. The invention provides a method for improving
cell surface display efficiency. The method comprises the following steps: S1, increasing the size of a
cell; knocking out genes in the cells to increase the size of the cells; the
gene is selected from a group consisting of a PASchr < 2-10508 >, a PASchr < 1-40585 >, a PASchr < 30819 >, a PASchr < 2-20036 >, a PASchr < 2-10366 >, a PASchr < 1-40244 >, a PASchr < 30688 >, a PASchr < 1-10383 >, a PASchr < 1-40081 > and a PASchr < 40223 >. According to the method for improving the cell
surface display efficiency, ten single-
gene knockout strains and ten double-
gene knockout strains are obtained based on the method, and experiments prove that the cell sizes of the strains are increased to different extents, so that the display efficiency, the display quantity and the
enzyme activity are improved in the aspect of cell surface display, and the cell surface display efficiency is improved. Meanwhile, the report
protein UGT73F24m-Ag alpha of the strains catalyzes GA to obtain a GLMG product, and the concentration of the GLMG product is increased to different degrees.