2results about How to "Stimulate immune response" patented technology

This invention discloses a recombinant protein for the prevention of Klebsiella pneumoniae, its gene, and its applications. The recombinant protein includes specific antigenic epitopes of Klebsiella pneumoniae OmpA protein and / or OmpX protein. The amino acid sequences of the specific antigenic epitopes of the Klebsiella pneumoniae OmpA protein are shown in SEQ ID NO.1, and the amino acid sequences of the specific antigenic epitopes of the Klebsiella pneumoniae OmpX protein are shown in SEQ ID NO.2. The recombinant protein can be efficiently expressed in a prokaryotic system and, after purification and refolding, exhibits good immunogenicity. Animal experiments show that this recombinant protein can induce high-titer specific antibodies and cellular immune responses in mice and provides significant protection against lethal doses of Klebsiella pneumoniae challenge. The single-component proteins KP-A and KP-X showed higher protection rates in the challenge experiment. The recombinant protein provided by this invention can be used to prepare vaccines or immunizing agents for the prevention and / or treatment of Klebsiella pneumoniae infection.

The invention relates to an application of an activity regulating cytoskeleton associated protein (ARC) in an REV nanoparticle vaccine and the vaccine, and belongs to the technical field of animal vaccine preparation, the amino acid sequence of the activity regulating cytoskeleton associated protein (ARC) is shown as SEQ ID NO.3, the nucleotide sequence is shown as SEQ ID NO.4, and the activity regulating cytoskeleton associated protein (ARC) is shown as SEQ ID NO.3. The activity regulation cytoskeleton associated protein is used as a carrier protein in a nanoparticle vaccine, and the nanoparticle vaccine is an REV nanoparticle vaccine. The ARC is used as a carrier in the nanoparticle vaccine for the first time to be self-assembled into nanoparticles, and the nanoparticles can serve as an antigen delivery platform, play a protein-antigen delivery role and stimulate an organism to generate immune response.