27results about How to "Stimulate immune response" patented technology

The invention belongs to the field of nano biomedicine, and particularly discloses a yeast bionic vaccine based on a metal organic framework ZIF-8 as well as a construction method and application of the yeast bionic vaccine, and the structure of the bionic vaccine is that ZIF-8 loaded with antigen OVA is encapsulated in a yeast shell. The invention relates to preparation and anti-tumor immune response of the bionic vaccine. The yeast shell is obtained by performing acid and alkali treatment and organic solvent washing on saccharomycetes and has the effects of targeting antigen presenting cells and stimulating immune response, and the antigen-loaded metal organic framework ZIF-8 is encapsulated in the yeast shell and has relatively good stability and an effect of continuously releasing antigens. The ZIF-8 is decomposed in antigen presenting cells, and lysosome escape of the antigen is induced. The yeast bionic vaccine provided by the invention can specifically target antigen presenting cells, continuously release tumor antigens in vivo, mediate escape of lysosome of the antigens and stimulate strong anti-tumor immune response, and has a better application prospect.

The invention discloses application of vimogib in preparation of a product for delaying and / or reversing T cell senescence, and belongs to the field of biological medicine. Experiments prove that the vimogii can enhance the lysosome function of T cells by enhancing the expression of TFE3 protein, so that the expression of anti-tumor and anti-virus related cytokines is enhanced, the lysosome function of senescent T cells of old people is improved, and the anti-tumor and anti-virus infection capability of the old people is enhanced. It is found for the first time that vimogii can improve the lysosome function of T cells by enhancing TFE3 protein expression, and the blank in the field is filled. According to the application, a new treatment thought is provided for improving immune aging related diseases of old people, the T cell lysosome function is enhanced by enhancing TFE3 protein expression through the medicine, then T cell aging is delayed and / or reversed, and an innovative application direction is provided for intervention of an aging immune system.

The invention provides a glycosyl-modified fusion protein, a nucleic acid molecule, an expression vector, a host cell and application. In the first aspect, the invention provides a glycosyl-modified fusion protein which comprises a mouse Fc variant and a prostatic cancer tumor antigen, the mouse Fc variant is obtained by performing amino acid mutation and non-mammalian glycosylation modification on a mouse Fc fragment; the mouse Fc variant comprises at least one of alanine at the 223 site, alanine at the 228 site, alanine at the 230 site, leucine at the 330 site and glutamic acid at the 332 site; the non-mammalian glycosylation modification does not comprise sialic acid modification; the site numbering is carried out according to an EU numbering system; the mouse Fc variant improves combination of a mouse Fc fragment and DC cells and DC activation, including proliferation and activation of specific T cells.

The invention provides a glycosyl-modified fusion protein, a nucleic acid molecule, an expression vector, a host cell and application. In the first aspect, the invention provides a glycosyl-modified fusion protein which comprises a mouse Fc variant and a polypeptide antigen, the mouse Fc variant is obtained by performing amino acid mutation and non-mammalian glycosylation modification on a mouse Fc fragment; the mouse Fc variant comprises at least one of alanine at the 223 site, alanine at the 228 site, alanine at the 230 site, leucine at the 330 site and glutamic acid at the 332 site; the non-mammalian glycosylation modification does not comprise sialic acid modification; the site numbering is carried out according to an EU numbering system; the mouse Fc variant improves combination of a mouse Fc fragment and DC cells and DC activation, including proliferation and activation of specific T cells.

The invention belongs to the field of veterinary traditional Chinese medicines, and provides a traditional Chinese medicine composition for enhancing immunity and treating chicken virus infection and a preparation method thereof. The traditional Chinese medicine composition is prepared from the following components in parts by weight: 18 to 22 parts of radix isatidis, 18 to 22 parts of indigo naturalis, 15 to 20 parts of radix angelicae sinensis, 10 to 15 parts of poria cocos, 10 to 15 parts of rhizoma alismatis, 8 to 12 parts of medicated leaven, 8 to 12 parts of radix salviae miltiorrhizae, 8 to 12 parts of radix codonopsis, 8 to 12 parts of rhizoma cyperi and 4 to 8 parts of liquorice root. The traditional Chinese medicine composition disclosed by the invention has outstanding performance in the aspect of improving IgG and IgM levels, and further shows that the traditional Chinese medicine composition disclosed by the invention can effectively stimulate immune response of an organism and enhance the resistance of the organism to viruses. Animal pharmacological experiments show that the compound has a remarkable treatment effect on chicken infectious disease anemia, avian leukosis and chicken adenovirus infection, provides a new treatment thought for treatment of chicken viral infection, and has a wide application prospect.

The invention belongs to the technical field of biological medicines, and discloses a chicken infectious anemia virus VP1 recombinant protein, a monoclonal antibody, a blocking ELISA kit and a preparation method of the blocking ELISA kit, pCold-His-TF-vp1 and pCold-His-TF-vp1 are used as prokaryotic expression vectors to establish chicken infectious anemia virus related recombinant proteins His-VP1 and StrepII-VP1, the expression of VP1 can be helped, and the recombinant proteins His-VP1 and StrepII-VP1 can be used for preparing the chicken infectious anemia virus related recombinant proteins His-VP1 and StrepII-VP1. Compared with the prior art, the method has the advantages that the method is simple and convenient to operate, good immunoreaction after immunization is facilitated, the monoclonal antibody with the correct epitope is generated, further, the monoclonal antibody with the correct epitope and the appropriate affinity can serve as a foundation for developing a blocking ELISA kit, and therefore a foundation can be laid for research on detection or diagnosis of the chicken infectious anemia virus.

The invention provides an antigen purification and renaturation method, which comprises the following steps: by improving a renaturation agent formula and a purification and renaturation process route, firstly completing chromatographic column purification after denaturation and then carrying out renaturation, and containing polyethylene glycol and cystine in an equilibrium buffer solution, a washing buffer solution, an elution buffer solution and a renaturation agent, the problem that recombinant protein is prone to aggregation and precipitation after renaturation is solved, CHAPS is further added to improve the renaturation effect for the recombinant protein with weak stability, the prepared recombinant protein is more stable, aggregation and precipitation are prevented, the yield is high, good immunocompetence is achieved, and the detection sensitivity when the recombinant protein is used for detecting antibodies is improved.

The invention relates to application of a dietary fiber probiotic composition, in particular to application of a composition of ganoderma lucidum dietary fibers and lactobacillus fermentum CEC-T5716 in preparation of drugs or health care products for preventing or relieving constipation. The constipation symptom can be effectively improved by influencing the weight of mice, the first black stool time, the excrement water content, the small intestine propulsion rate, the colon tissue state, colon tissue aquaporin, the intestinal barrier, inflammatory factor related genes and excrement intestinal flora expression and adjusting the short-chain fatty acid content in cecum contents; the symptoms of dry and hard excrement, prolonged defecation time and the like caused by constipation are relieved, the intestinal peristalsis capability of mice with constipation can be promoted, and the damage of constipation to intestinal tracts is relieved.

The invention discloses an application of SseB protein in preparation of a vaccine for cross-immunization homologous or heterologous serotype salmonella enteritidis. Animal experiments are used for evaluating the immune protection capability of the salmonella enteritidis SseB protein, and results show that the salmonella enteritidis SseB protein has good immunoreactivity and can remarkably relieve clinical symptoms caused by homologous or heterologous serotype wild strains of salmonella enteritidis. The salmonella enteritidis SseB protein with the cross immune protection effect, provided by the invention, has the potential of developing a salmonella enteritidis subunit vaccine.

The invention belongs to the field of medicines, and particularly relates to a recombinant protein vaccine for preventing and treating SARS-CoV-2 variants JN.1, BA.2. 86 and XBB pedigree, a combined medicine and application. In order to solve the problem that Aumitron JN.1, BA.2. 86 and XBB pedigree variants show significant immune escape and blot immune phenomena after previous vaccination and virus infection, the invention provides an XBB.1. 5 recombinant protein vaccine, which induces powerful body fluid, cell and mucous membrane immune responses to JN.1, BA.2. 86 and XBB pedigree variants by intramuscular and intranasal vaccination. Compared with homologous vaccine inoculation, the inactivated or mRNA vaccine is applied and then heterologous vaccine inoculation is carried out by using the vaccine, so that better immune response is obtained. And the vaccine induces effective protective immunity against the attack of the live Omitron EG.5.1 virus in vivo. Therefore, the XBB.1.5 vaccine disclosed by the invention has clinical use value.

Compositions, methods, and kits of small molecular weight agents are capable of binding and stimulating Fc-gamma-activated receptors to enhance humoral immune responses and immune effector cell responses by improving immune effector cell and / or antibody-based efficacy on pathogenic microorganisms as well as viral infected and dysregulated cells. These can be used to treat infectious diseases, cancer and other humoral immunosuppressive diseases. In addition, non-stimulating analogs capable of blocking Fc-gamma activated receptors can also be used to inhibit immune effector cell activation and inflammatory downstream mediators.

The present invention belongs to the field of biology and medicine and the field of pharmaceutical preparation technology, and specifically relates to the preparation and application of a gold nanoparticle and its liposome. The present invention prepares an asymmetrically modified gold nanoparticle (HAM) of arginine and maleimide groups, and an active targeted liposome HAM‑αLip encapsulating gold nanoparticles. On the one hand, the large-diameter targeted modified liposomes ensure that they will not be cleared by the reticuloendothelial system in the blood circulation system, ensuring the accumulation of HAM‑αLip in the tumor site; on the other hand, the modified gold nanoparticles are released when they reach the tumor site, achieving deep penetration of the tumor and improving the anti-tumor treatment effect.

The invention provides mRNA (messenger ribonucleic acid), an mRNA vaccine preparation as well as a preparation method and application of the mRNA vaccine preparation, and belongs to the technical field of biological medicines. The amino acid sequence of the mRNA provided by the invention is as shown in SEQ ID NO. 2. Research finds that an mRNA vaccine preparation prepared from mRNA with an amino acid sequence as shown in SEQ ID NO.2 can effectively stimulate dendritic cells to mature and secrete proinflammatory cytokines, and further activate effector T cells to kill prostatic cancer. The method for preparing the mRNA vaccine preparation is simple and can be used for industrial production. According to the invention, the encapsulation efficiency of the mRNA vaccine preparation prepared by taking the liposome nanoparticles as a carrier is high, so that an organism can be effectively stimulated to generate immune response, and the immunogenicity is high.

Provided herein are poultry diets comprising a phytase polypeptide or fragment thereof, wherein the diet is free or substantially free of inorganic phosphate.

This invention discloses a recombinant protein for the prevention of Klebsiella pneumoniae, its gene, and its applications. The recombinant protein includes specific antigenic epitopes of Klebsiella pneumoniae OmpA protein and / or OmpX protein. The amino acid sequences of the specific antigenic epitopes of the Klebsiella pneumoniae OmpA protein are shown in SEQ ID NO.1, and the amino acid sequences of the specific antigenic epitopes of the Klebsiella pneumoniae OmpX protein are shown in SEQ ID NO.2. The recombinant protein can be efficiently expressed in a prokaryotic system and, after purification and refolding, exhibits good immunogenicity. Animal experiments show that this recombinant protein can induce high-titer specific antibodies and cellular immune responses in mice and provides significant protection against lethal doses of Klebsiella pneumoniae challenge. The single-component proteins KP-A and KP-X showed higher protection rates in the challenge experiment. The recombinant protein provided by this invention can be used to prepare vaccines or immunizing agents for the prevention and / or treatment of Klebsiella pneumoniae infection.

The invention belongs to the technical field of vaccines, and provides a canine distemper subunit vaccine spray which is formed by dissolving nanoparticles carrying key antigen epitope peptide in normal saline in the form of freeze-dried powder. Comprising nanoparticles carrying key antigen epitope peptide and a buffer solution, and the nanoparticles are dissolved in the buffer solution in the form of freeze-dried powder; the mass ratio of the nanoparticles to the buffer solution in the spray is (0.1-10): 1; the nano-particles are composed of epitope peptide of the hemagglutinin protein of the canine distemper virus and a nano-carrier. The spray type canine distemper vaccine is convenient to store and transport, meanwhile, the stability of antigens, the convenience of inoculation and the safety and effectiveness of the vaccine are guaranteed, and large-scale inoculation of the vaccine can be rapidly promoted.

The present invention discloses an incubation device and a chemiluminescent immunoassay analyzer having the same, wherein the incubation device comprises a base assembly, an incubation mechanism and a mobile drive mechanism; the base assembly comprises a frame and a first guide member arranged on the frame; the incubation mechanism is used to place a reaction cup; the mobile drive mechanism comprises a drive assembly and a mobile assembly driven and connected to the drive assembly, the mobile assembly comprises a first connecting seat and a first rolling wheel rotatably arranged on the first connecting seat, the first connecting seat is fixedly connected to the incubation mechanism, the first rolling wheel is connected to the first guide member and can roll along the guide direction of the first guide member, and the drive assembly can drive the mobile assembly to reciprocate along the guide direction of the first guide member. The present invention sets the first rolling wheel to roll and guide on the first guide member, and the interaction force therebetween is small, which can reduce the vibration of the base assembly, reduce the generation of noise, and improve the comfort of the working environment.

A vaccine carrier based on aluminum hydroxide nanoparticles, which is formed by compounding PEG derivative materials with aluminum salts to form nanoparticles. It not only retains the powerful Th2 humoral immune adjuvant properties of aluminum salts, but can also be efficiently delivered to the draining lymph nodes in the body, and is easily taken up by dendritic cells (DCs), effectively cross-presents, induces cellular immune responses, and has a strong Th1 immune response.

The invention belongs to the field of medicines, and particularly relates to a vaccine adjuvant and application thereof. And the adjuvant contains one or more of total saponins of Hylocereus undatus, a saponin component HA and a saponin component HB. The total saponin and the monomer saponin with immunologic adjuvant activity are separated from the Honeysuckle flower, and the total saponin and the monomer saponin can non-specifically change or enhance immune response reactions of various vaccines including influenza vaccines, so that not only can humoral immunity of influenza vaccine immunized mice be enhanced, but also cellular immunity of the influenza vaccine immunized mice can be enhanced.

The invention discloses application of mesoporous silica nanoparticles to improvement of salt tolerance of strignum leptosphaereum, and belongs to the technical field of plant stress resistance. Wherein the particle size of the mesoporous silica nanoparticles is 80-120 nm, and the mesoporous silica nanoparticles are in negative potential. The mesoporous silica nanoparticles provided by the invention can effectively reduce the content of active oxygen increased due to salt stress, relieve the salt stress by reducing accumulation of the active oxygen, and also can promote ion balance to enhance the salt stress resistance of the acidichrocephalum leptosphaerocephalum, so that the salt stress resistance of the acidichrocephalum leptosphaerocephalum is improved. Meanwhile, the preparation process of the mesoporous silicon dioxide nanoparticles is relatively simple, environment-friendly, low in cost and free of toxic and side effects, and a foundation is laid for exploring the stress resistance and physiological process of a nano material on plants in the mode of phytoncigia palustris.

The invention relates to an application of an activity regulating cytoskeleton associated protein (ARC) in an REV nanoparticle vaccine and the vaccine, and belongs to the technical field of animal vaccine preparation, the amino acid sequence of the activity regulating cytoskeleton associated protein (ARC) is shown as SEQ ID NO.3, the nucleotide sequence is shown as SEQ ID NO.4, and the activity regulating cytoskeleton associated protein (ARC) is shown as SEQ ID NO.3. The activity regulation cytoskeleton associated protein is used as a carrier protein in a nanoparticle vaccine, and the nanoparticle vaccine is an REV nanoparticle vaccine. The ARC is used as a carrier in the nanoparticle vaccine for the first time to be self-assembled into nanoparticles, and the nanoparticles can serve as an antigen delivery platform, play a protein-antigen delivery role and stimulate an organism to generate immune response.

The invention discloses a melamine hapten, an artificial antigen and an antibody as well as a preparation method and application thereof, and relates to the technical field of immunochemistry and food safety detection. The hapten is prepared from 2-chloro-4, 6-diamino-1, 3, 5-triazine and 8-amino caprylic acid through a reaction, the artificial antigen is prepared from the hapten, N, N '-dicyclohexylcarbodiimide (DCC) and N-hydroxysuccinimide (NHS), and the antibody is prepared from an animal immunized by the artificial antigen. The antigen and the antibody can be applied to preparation of a melamine colloidal gold immunochromatography test strip, and can be used for rapidly and sensitively detecting melamine residues in animal-derived food.

The invention belongs to the technical field of aquaculture, and provides a growth-promoting disease-resistant natural astaxanthin conditioner and a preparation method thereof. According to the growth-promoting and disease-resisting natural astaxanthin conditioner provided by the invention, the fermented astaxanthin is combined with the seaweed active extract and the deep sea fish oil for preparation, and is compounded with the compound probiotic agent, the natural mineral salt and the like, so that the efficacy can be greatly improved, the synergistic and complementary effects of all the components in the aspects of activity and bioavailability are fully exerted, and the conditioner is easier to absorb and free of side effects; the feed can accelerate nutrient absorption of fish bodies, promote color development, enhance immunity of the fish bodies, prevent diseases and remove bacteria, and is helpful for healthy and rapid growth of aquarium fish.

The present invention provides an mRNA, an mRNA vaccine preparation, and a preparation method and application thereof, which belong to the field of biomedical technology. The amino acid sequence of the mRNA of the present invention is shown in SEQ ID NO.2. The present invention has found that the mRNA vaccine preparation prepared from the mRNA with the amino acid sequence as shown in SEQ ID NO.2 can effectively stimulate the maturation of dendritic cells and the secretion of proinflammatory cytokines, thereby activating effector T cells to kill prostate cancer. The method for preparing the mRNA vaccine preparation of the present invention is simple and can be used for industrial production. The present invention uses liposome nanoparticles as carriers, and the encapsulation rate of the mRNA vaccine preparation is high, so that the body can be effectively stimulated to produce an immune response, and the immunogenicity is high.

The invention provides a preparation method of a polysaccharide vaccine diluent and application of the polysaccharide vaccine diluent to a Newcastle disease vaccine, and belongs to the technical field of biological pharmacy. The invention provides an Acanthopanax southern polysaccharide vaccine diluent and a preparation method thereof, and solves the problems that the titer uniformity of an antibody generated by diluting a vaccine by using normal saline or a phosphate buffer solution is not high, a stress reaction and an anaphylactic reaction are generated on an organism after inoculation, the immune effect of the vaccine is influenced, and the like. The acanthopanax southern polysaccharide vaccine diluent can be applied to animal vaccines, including Newcastle disease vaccines, and solves the problem that no polysaccharide diluent for Newcastle disease vaccines exists in the prior art.

The present invention relates to a recombinant gene of a rabies virus G protein virus-like particle, a virus-like particle, and a construction method and application thereof. The nucleotide sequence of the recombinant gene is shown in SEQ ID NO: 1. The present invention is based on a lentiviral expression system, optimizes the design of rabies virus envelope protein, replaces its transmembrane structure with the transmembrane structure of the GP5 protein of blue ear virus, and the prepared virus-like particle not only has the biological characteristics of the natural rabies virus, but also only contains a single fragment G protein that produces neutralizing antibodies, is safe and effective, has high immunogenicity, and the prepared virus-like particle has a high secretion expression amount, and can be used as a candidate vaccine for a new virus rabies virus vaccine.