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373 results about "Messenger ribonucleic acid" patented technology
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Messenger RNA. An RNA (ribonucleic acid) molecule which has coding regions and translation signals derived from a gene, carries the reverse template message from DNA, and is required for protein synthesis. mRNA is a nucleic acid intermediate that specifies the amino acid sequence of a polypeptide during translation.
Neurotrophic factor production promoting device
ActiveUS20090326315A1Easy to produceElectrotherapyMagnetotherapy using coils/electromagnetsDiseaseExocytosis
A neurotrophic factor production promoting device is provided, which is able to promote production of a neurotrophic factor or neurotrophic factor-like substance in an affected area by a simple technique that, regardless of the place of treatment, can be performed without transplantation of cells or injection into the affected area, in order to prevent or treat various diseases such as brain diseases. In order to apply a high frequency alternating magnetic field in the range of 20 MHz to 180 MHz, 280 MHz to 600 MHz, or 700 MHz to 1000 MHz to cells at a magnetic flux density of no more than 0.01 Tesla, the neurotrophic factor production promoting device includes a high frequency electromagnetic wave generating means generating a high frequency electromagnetic wave of the abovementioned frequency, in which the magnetic stimulation by the high frequency alternating electromagnetic field of the abovementioned high frequency allows the intracellular concentration of calcium ions to be increased so that exocytosis of the neurotrophic factor group is induced, and the magnetic stimulation allows messenger ribonucleic acid (mRNA) of the neurotrophic factor group to be increased in the cells so that the synthesis and extracellular release of the neurotrophic factor group are promoted.
Owner:P MIND
SNP (Single Nucleotide Polymorphism) molecular marker related with back fat thickness trait of pig and detection method of SNP molecular marker
ActiveCN105255870AEarly detectionQuick checkMicrobiological testing/measurementDNA/RNA fragmentationEnzyme digestionNucleotide
The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker related with a back fat thickness trait of a pig and a detection method of the SNP molecular marker. By detecting exon regions of MARK4 (Microtubule Affinity-Regulating Kinase) genes of different pig species, one SNP locus (G-A) is found to exist at 55th basic group (mRNA (Messenger Ribonucleic Acid) total-length 2581th base group) in 3 minute UTR (Untranslated Region) of the MARK4 gene, an allele A is positively correlated with low back fat trait, and an allele G is positively correlated with high back fat trait. The invention provides the method for detecting the SNP molecular marker. Pig genomic DNA (Deoxyribonucleic Acid) to be detected is amplified by using a primer pair with the nucleotide sequence shown in SEQ ID NO.1-2 and is subjected to enzyme digestion by using pstI; if an enzyme-digested product is 251bp, a base group at a mutation is A; if the enzyme-digested product is 218bp, a base group at the mutation is G. According to the method provided by the invention, the back fat thickness of the pig can be predicted early, quickly and effectively at low cost; the SNP molecular marker can be used as a useful molecular marker for genetic improvement of the pig species.
Owner:湘潭市家畜育种站
Methods for therapeutic administration of messenger ribonucleic acid drugs
ActiveUS20180256628A1Reducing and inhibiting accelerated blood clearanceBlood clearance is reduced and inhibitedOrganic active ingredientsNucleic acid vectorAntiendomysial antibodiesBinding site
The disclosure features methods of reducing or inhibiting an anti-drug antibody response in a subject, as well as methods of reducing or inhibiting unwanted immune cell activation in a subject to be treated with a messenger RNA (mRNA), comprising administering to the subject a mRNA, e.g., a chemically modified messenger RNA (mmRNA), encoding a polypeptide of interest, wherein the mRNA comprises at least one microRNA (miR) binding site for a miR expressed in immune cells, such as miR-126 binding site and / or miR-142 binding site, such that an anti-drug antibody response to the polypeptide or interest, or unwanted immune cell activation (e.g., B cell activation, cytokine secretion), is reduced or inhibited in the subject. The disclosure further provides therapeutic treatment regimens designed to reduce or inhibit AD A or unwanted immune cell activation (e.g., B cell activation, cytokine secretion) in a subject being treated with mRNA-based therapeutics.
Owner:MODERNATX INC
Method for establishing immunodeficiency mouse model
ActiveCN103409468AShorten the timeStrain background pureMicroinjection basedAnimal husbandryReverse transcriptaseImmunodeficient mouse model
The invention discloses a method for establishing an immunodeficiency mouse model. The method comprises the following steps of: (1) constructing DNA (deoxyribonucleic acid) which enables functions of an immune related gene in a mouse cell to be lost; (2) purifying mRNA (messenger ribonucleic acid), and cryopreserving in a super-purified water of RNAase free; (4) feeding a pregnant female mouse and a pseudopregnant female mouse under an SPF (specific pathogen free) level feeding condition; (5) taking a fallopian tube from the abdominal cavity of the pregnant female mouse in the step (4), and collecting fertilized ovum; (6) injecting the mRNA obtained in the step (3) into the pronucleus of the fertilized ovum in the step (5) by using a microinjection method; (7) transplanting the fertilized ovum obtained in the step (6) into the body of the pseudopregnant female mouse obtained in the step (4), and enabling the pseudopregnant female mouse to culture the second generation; (8) establishing a stable deficient mouse strain. The method has the advantages that a mouse model which completely does not have immunological rejection to allotransplantation can be obtained for testing.
Owner:湖南昭泰生物医药有限公司
Micro-droplet type PCR (polymerase chain reaction) chip and manufacture method thereof
InactiveCN103451088AFast fusionImprove throughputBioreactor/fermenter combinationsBiological substance pretreatmentsA-DNAOil phase
The invention provides a micro-droplet type PCR (polymerase chain reaction) chip and a manufacture method thereof. The upper layer of the chip is a PDMS (polydimethylsiloxane) imaging cover plate formed by connecting a DNA (deoxyribonucleic acid) target fragment with a primer injection port, a single phase drop forming microstructure, a PCR reaction enzyme and an mRNA (messenger ribonucleic acid) substrate injection port, an oil phase reagent injection port, a drop straight line channel, a cross hybrid channel, a micro drop buffer channel, a micro drop PCR reaction chamber and a micro drop PCR waste liquid chamber, the lower layer of the chip is a glass substrate, and the upper layer and the lower layer are bonded and packaged to form a complete micro drop PCR chip. The manufacture method of the chip comprises the steps of firstly, manufacturing a mask plate; secondly, molding; thirdly, pouring a die; and fourthly, taking a figure. The PDMS figure at the upper layer of the chip adopts a multilayer filter structure, and the formed micro drop has a uniform size, stable grain diameter and strong controllability. Compared with traditional PCR chips, the micro drop PCR chip manufactured by the method has great advantages in hybrid effect, reaction efficiency, and high throughput.
Owner:SHANGHAI JIAO TONG UNIV
Pharmaceutical compositions and treatment of genetic diseases associated with nonsense mediated RNA decay
Owner:NEW YORK UNIVERSITY
Application of hydrogen sulfide donor to preparation of medicine for treating central nervous system disease
ActiveCN102078327AAlleviate learning and memory lossPromote learning and memoryOrganic active ingredientsNervous disorderTumor necrosis factor alphaSodium hydrosulfide
The invention belongs to the field of pharmacy, and relates to application of a hydrogen sulfide donor to the preparation of a medicine for treating central nervous system disease, in particular to application of the hydrogen sulfide donor, namely sodium hydrosulfide, or allyl cysteine and analogues thereof to the preparation of the medicine for treating the central nervous system disease. Integral animal model experiments prove that the sodium hydrosulfide and propargyl cysteine can reduce the learning and memory impairment of rats, increase the content of hydrogen sulfide in hippocampus tissues of the rats, inhibit the messenger ribonucleic acid (mRNA) expression of a tumor necrosis factor alpha and a receptor I thereof, inhibit an inflammatory mediator in the hippocampus tissues of rats with dementia and inhibit inflammation-related enzymes in the hippocampus tissues of the rats, and can be used as a medicine for treating Alzheimer disease and other inflammation-related central nervous system degenerative diseases such as Parkinson disease.
Owner:FUDAN UNIV
Silkworm fibroin heavy-chain gene mutation sequence and mutation method and application
ActiveCN102358902AHigh expressionHigh purityFermentationVector-based foreign material introductionGeneticsSilk gland
The invention relates to a silkworm fibroin heavy-chain gene mutation method, which specifically comprises the step of: acting mRNA (Messenger Ribonucleic Acid) of a coded zinc-finger nuclease sequence on loci 1325-1362 of a silkworm fibroin heavy-chain gene shown as SEQ ID NO:1 to form target positions for recognizing zinc-finger nuclease, thereby obtaining a series of silkworm fibroin heavy-chain mutated genes; and the mutated sequence can be applied to preparation of sericin and extrinsic proteins. Mutants provided by the invention have the following advantages that: (1) a posterior division of silkgland of a fibroin heavy-chain gene mutant provided by the invention serious degrades, a cocoon shell only contains the sericin synthesized and secreted by a middle division of silkgland, if the mutated strains are utilized to transgenically express the extrinsic proteins, the expressed amount and the purity of the extrinsic proteins can be greatly increased, and thus, a brand-new useful genetic material is provided for the development of a silkworm fibroin bioreactor; and (2) the cocoon shell of the mutated strain provided by the invention only contains the sericin, and thus, a new source is provided for the large-scale production of the sericin.
Owner:SOUTHWEST UNIV
Selection of antigen-specific t cells
InactiveUS20090004742A1Altering cellular behaviorPeptide/protein ingredientsVertebrate cellsDiagnostic Radiology ModalityDendritic cell
The requirement of T cell activation for efficient expression of genes after messenger ribonucleic acid (mRNA) transfection is leveraged to identify and enrich antigen-specific T cells responding to antigen-pulsed dendritic cells (DCs). RNA transfection of marker genes is used for the selection and enrichment of antigen-specific T cells for use in adoptive immunotherapy. RNA-modified T cells are also used for the generation of enhanced effector populations for use in adoptive immunotherapy. Genes whose transient expression may significantly enhance the in vivo function of T cells (i.e., migratory receptors, anti-apoptotic genes or cytokines enhancing T cell proliferation / differentiation) are used in this modality.
Owner:DUKE UNIV
Porcine heat stress resistance selenium-rich composite bacteria feed additive and application thereof
ActiveCN102823772AIncrease daily weight gainReduce Hsp70Animal feeding stuffBiotechnologyFeed additive
The invention belongs to the field of application of feed additive, and relates to a porcine heat stress resistance selenium-rich composite bacteria feed additive and application thereof. Saccharomyces cerevisiae, lactobacillus acidophilus and streptococcus thermophilus are selected as strains, inoculated in a proper fermentation broth containing sodium selenite, transformation of selenium and probiotics growth accelerating agent in a volume ratio of 1:2:2, and fermented for 30 to 40 hours at a temperature of between 20 and 40 DEG C, throughput of 0.05m<3> / h, revolution of 70r / min and tank pressure of 0.04Mpa; and the selenium enriched composite bacteria preparation can be prepared through proper subsequent treatment. The daily gain and feed-weight ratio of a pig can be remarkably improved in a high-temperature environment by feeding the preparation, the oxidation resistance and immunity functions can be enhanced, the mRNA (messenger ribonucleic acid) level of heat shock proteins Hsp70 and Hsp27 can be reduced, and the effect of porcine heat stress resistance can be effectively played.
Owner:NANJING AGRICULTURAL UNIVERSITY
Imaging individual mRNA molecules using multiple singly labeled probes
A method for probing a target sequence of messenger ribonucleic acid molecules (mRNA's) in a fixed, permeabilized cell, said target sequence including at least 30 non-overlapping probe binding regions of 15-100 nucleotides, comprising immersing said cell in an excess of at least 30 nucleic acid hybridization probes, each singly labeled with the same fluorescent label and each containing a nucleic acid sequence that is complementary to a different probe binding region of said target sequence; washing said fixed cell to remove unbound probes; and detecting fluorescence from said probes.
Owner:RUTGERS THE STATE UNIV
Recombinant oral protein TAT-GH of tilapia, preparation method for recombinant oral protein TAT-GH and application of recombinant oral protein TAT-GH
ActiveCN102703483AGuaranteed accuracyIntegrity guaranteedBacteriaAnimal feeding stuffBiotechnologyEscherichia coli
The invention discloses recombinant oral protein TAT-GH of tilapia, a preparation method for the recombinant oral protein TAT-GH and application of the recombinant oral protein TAT-GH. The method comprises the following steps of: extracting total messenger ribonucleic acid (mRNA) from a pituitary gland of male tilapia, performing reverse transcription-polymerase chain reaction (RT-PCR) to obtain complementary deoxyribonucleic acid (cDNA), performing PCR amplification to obtain a growth hormone (GH) gene of the tilapia, and amplifying a TAT-GH gene by a PCR overlap extension method; constructing a recombinant plasmid pET-32a(+)-TAT-GH; and transforming Escherichia coli BL21 (DE3) by using the recombinant plasmid pET-32a(+)-TAT-GH, efficiently expressing fusion protein TAT-GH in the form of an inclusion body under the induction of isopropyl thiogalactoside (IPTG), and purifying and renaturing to obtain active protein. The protein can effectively promote the growth and development of fries of the tilapia after being fed to the fries of the tilapia.
Owner:HUBEI TAIYANGHONG BIOLOGICAL TECH CO LTD
Panda source lactobacillus plantarum and application
InactiveCN106434411AProtection from damageReduce percentageAntibacterial agentsBacteriaEscherichia coliTLR4
The invention discloses a panda source lactobacillus plantarum and application. The strain was preserved in China Center for Type Culture Collection on May 4, 2016 and the preservation number is CCTCC NO: M2016245. The panda source lactobacillus plantarum provided by the invention can be used for improving antibody levels of IgA, IgM and IgG immune globulins of blood serum; a condition that the percent of lymphocytes is reduced and the percent of neutrophils is increased, caused by infection and induction of enterotoxigenic escherichia coli, is inhibited; expression levels of inflammation related factors IL-1beta, IL-8, IL-6, TLR4 and MyD88mRNA (messenger Ribonucleic Acid) are remarkably down-regulated, and intestinal acute inflammation response caused by the enterotoxigenic escherichia coli is alleviated.
Owner:SICHUAN AGRI UNIV +1
Soybean cyst nematode resistance gene and application thereof
InactiveCN102206651AEffective guidanceEffectively guide conventional breedingFungiBacteriaGlycineAgricultural science
The invention relates to a soybean cyst nematode resistance gene and application thereof. The cDNA (complementary deoxyribonucleic acid) fragment (SRC-J-6) provided by the invention is derived from soybean (Glycine max (L.) Merrill.) variety L-10, and has a nucleotide sequence shown in 1) a nucleotide sequence shown in SEQ ID NO. 1; 2) a DNA (deoxyribonucleic acid) molecule capable of hybridizingwith the nucleotide sequence shown in SEQ ID NO. 1 and being expressed under strict conditions; 3) an mRNA (messenger ribonucleic acid) molecule, which has homology of more than 90% with the nucleotide sequence shown in 1) and can be expressed; or 4) a DNA (deoxyribonucleic acid) molecule, which has homology of more than 90% with the nucleotide sequence shown in 1) and can be expressed. After theplant tissues are transformed by the gene disclosed by the invention, the resistance to cyst nematode of the transgenic soybean is significantly improved. The soybean cyst nematode resistance gene provided by the invention has important significance, not only can effectively guide conventional breeding, can also provide excellent genetic resources for the transgenic breeding of soybean and has broad application prospects in the soybean production.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY
Application of EPS8L3 gene expression detecting reagents to preparing osteoporosis diagnostic products
ActiveCN105112551AAchieve early diagnosisReduce mortalityMicrobiological testing/measurementDisease diagnosisOsteopetrosisOsteoporosis
The invention discloses a molecular marker for diagnosing osteoporosis. The molecular marker is an EPS8L3 gene. The molecular marker has the advantages that as proved by research, the mRNA (messenger ribonucleic acid) expression level of EPS8L3 genes in the blood of osteoporosis patients is obviously reduced as compared with normal persons; reagent kits for diagnosing the osteoporosis can be prepared according to correlation between the EPS8L3 genes and the osteoporosis and can be clinically widely applied.
Owner:GUAN BOJIAN BIOTECH CO LTD
Kit for jointly detecting breast cancer 21 genes and preparation method of kit
InactiveCN104004844AEasy to operateShorten detection experiment timeMicrobiological testing/measurementSingle sampleBAG1
The invention discloses a kit for jointly detecting breast cancer 21 genes. A PCR (polymerase chain reaction) amplification primer consists of 21 primer pairs, and can be used for jointly detecting 21 gene-related expression quantities: Ki67, STK15, Survivin, CCNB1, MYBL2, MMP11, CTSL2, GRB7, HER2, GSTM1, CD68, BAG1, ER, PGR, BCL2, SCUBE2, ACTB, GAPDH, PRLPO, GUS, and TFRC. Output fragments of the 21 pairs of primers related in the invention are smaller than 100bp, so that PCR efficiency after reverse transcription of mRNA (messenger ribonucleic acid) extracted by a paraffin specimen is greatly improved, all primers of 21 genes are premixed, and time and labor for designing and synthesizing 21 pairs of genes by an experimenter are saved; the corresponding primers of the 21 genes are not needed to be added for premixing in mother liquor, and therefore, errors of reaction holes are greatly reduced. The kit disclosed by the invention is simple to operate, and capable of detecting on a quantitative PCR instrument by only adding a template, so that detection experimental time is greatly shortened. The kit disclosed by the invention is applicable to a single sample and multiple samplers, so that results can be quickly obtained, and therefore, scientific research and clinical detection requirements can be satisfied very well.
Owner:杭州美中疾病基因研究院有限公司
LncRNA (long noncoding ribonucleic acid) excavating method based on gene sequence expression analysis
The invention finds a bioinformatics method according to gene sequence expression and gene prediction algorithms, wherein the method can be used for directly predicting and quantifying a long noncoding RNA (ribonucleic acid) and directly locking the LncRNA for further experimental verification. The method disclosed by the invention mainly comprises the following process: step one, collecting all the full-length mRNA (messenger ribonucleic acid) sequence data of a person; step two, removing the mRNA sequence comprising an extron of a coding protein; step three, forming a retrievable database by using LncRNA more than 200bp, and; step four, searching the existing gene expression sequence analysis data to identify over-expressed LncRNA from the analysis data; and step five, carrying out experimental verification. Thus, the over-expressed LncRNA in a specific cell tissue is predicted finally..
Owner:SHANGHAI CLUSTER BIOTECH
Single gene mRNA (messenger ribonucleic acid) methylation level detection method
The invention discloses a single gene mRNA (messenger ribonucleic acid) methylation level detection method. The method includes the steps of 1), designing fluorescent quantitative primers in a methylated region of a target gene; 2), extracting total RNA and fragmenting the total RNA into RNA fragments by a chemical fracture method; 3), enabling the RNA fragments obtained in the step 2) to precipitate all mRNA fragments with m6A loci through antibodies of specific recognition m6A, and collecting and enriching by agarose magnetic beads; 4), subjecting the mRNA fragments obtained by enrichment of the antibodies in the step 3) to reverse transcription, and using the fluorescent quantitative PCR (polymerase chain reaction) primers in the step 1) for real-time fluorescent quantitative PCR so as to obtain a relative expression quantity of the methylated target gene, wherein the relative expression quantity represents single gene mRNA methylation level.
Owner:ZHEJIANG UNIV
Transgenic zebrafish with specific myelin sheath removal capacity as well as preparation method and application thereof
InactiveCN103966261AIn-vivo testing preparationsVector-based foreign material introductionNucleotideWild type
The invention discloses transgenic zebrafish with specific myelin sheath removal capacity as well as a preparation method and an application thereof. The preparation method comprises the following steps: recombinant plasmid mbp-nfsB-EGFP and a Tol 2 transposase mRNA (messenger ribonucleic acid) are jointly introduced into wild zebrafish; and a zebrafish strain with stable inheritance is cultured and obtained, and the zebrafish strain with stable inheritance is the transgenic zebrafish with specific myelin sheath removal capacity, wherein the nucleotide sequence of an mbp gene promoter carried in the recombinant plasmid mbp-nfsB-EGFP is represented as SEQ ID NO.1. With adoption of the method, the transgenic zebrafish which can specifically remove myelin sheath, cause myelinoclasis and has stable inheritance capacity can be prepared and obtained effectively, under the action of metronidazole, oligodendrocyte of juvenile transgenic zebrafish can be removed specifically and the juvenile transgenic zebrafish has pathologic change of myelinoclasis together with decreased motor function, therefore, the transgenic zebrafish can provide a forceful animal model for a regulatory mechanism for viviperception of myelin sheath damage and regeneration and related drug screening.
Owner:GENERAL HOSPITAL OF PLA +1
Imaging individual mRNA molecules using multiple singly labeled probes
A method for probing a target sequence of messenger ribonucleic acid molecules (mRNA's) in a fixed, permeabilized cell, said target sequence including at least 30 non-overlapping probe binding regions of 15-100 nucleotides, comprising immersing said cell in an excess of at least 30 nucleic acid hybridization probes, each singly labeled with the same fluorescent label and each containing a nucleic acid sequence that is complementary to a different probe binding region of said target sequence; washing said fixed cell to remove unbound probes; and detecting fluorescence from said probes.
Owner:RUTGERS THE STATE UNIV
Alpha amylase and aspergillus niger strain for expressing same
ActiveCN103695388ALarge market spaceFungiMicroorganism based processesAlpha-amylaseMicrobiological culture
The invention aims to provide alpha amylase and an aspergillus niger strain for expressing the same. An amino acid sequence of alpha amylase is SEQ ID NO:1. A coding mRNA (messenger ribonucleic acid) sequence of alpha amylase is SEQ ID NO:2. The mutant strain aspergillus niger 1-B5 obtained by an ultraviolet mutagenesis method can efficiently express alpha amylase AclaP11 derived from aspergillus clavatus, with strain number of CGMCC (China General Microbiological Culture Collection Center) No.8443. The enzyme activity of aspergillus niger 1-B5 is 1.97 times that of an original strain. The optimum pH value and optimum temperature of alpha amylase AclaP11 expressed by aspergillus niger 1-B5 are respectively 6.0 and 50 DEG C. Compared with the original strain, aspergillus niger 1-B5 has the advantage that the enzymatic properties are not changed. Besides, aspergillus niger 1-B5 is a food safety fungus (GRAS(generally recognized as safe)), so that alpha amylase expressed by aspergillus niger 1-B5 in a secretory manner can be applied to the field of food additives, thus further broadening the market space of alpha amylase.
Owner:山东中科生物创新产业园管理有限公司
Carbohydrate composition with wound healing promoting effect and application of carbohydrate composition
ActiveCN108553481ARaw materials are easy to getEasy to manufactureOrganic active ingredientsDermatological disorderCarbohydrate compositionOligosaccharide
The invention provides a carbohydrate composition with a wound healing promoting effect and application of the carbohydrate composition. The carbohydrate composition contains at least five types of carbohydrate including carboxymethyl chitosan, rhizoma bletillae polysaccharides, fucoidan from fucus vesiculosus, heparan sulfate, chondroitin sulfate, oligosaccharides of hyaluronan and gulose aldehyde acid oligosaccharides. The carbohydrate composition and the application have the advantages that VEGFA (vascular endothelial growth factor A), FGF2 (fibroblast growth factor 2) and VE-cadherin protein mRNA (messenger ribonucleic acid) expression can be obviously improved by the carbohydrate composition, VEGF (vascular endothelial growth factor) secretion and skin fibroblast (HSF) and human immortalized keratinocyte (HaCaT) proliferation can be obviously promoted by the carbohydrate composition, and the obvious wound healing promoting effect can be realized by the carbohydrate composition; raw materials required by the carbohydrate composition are easily available, composition methods for the carbohydrate composition are simple, the carbohydrate composition is low in cost, easy to industrialize and wide in application range, and the like.
Owner:OCEAN UNIV OF CHINA
Culture method free of multipotential stem cell without serum and feeder layer
ActiveCN103555661AMaintain pluripotencyMaintain normal stateEmbryonic cellsGerm cellsSuspension cultureMessenger ribonucleic acid
The invention relates to a novel method for culturing a multipotential stem cell without serum and a feeder layer. The method is stable, reliable, and can culture the multipotential stem cell for a long time and also can maintain the self-renewing of the multipotential stem cell. The method is characterized in that a culture system with clear chemical components and without serum and the feeder layer is adopted to perform suspension culture on the multipotential stem cell. According to the morphology of cultured stem cells after 15 generations, mRNA (Messenger Ribonucleic Acid) and protein expression of multipotential marker molecule, and analysis on cell chromatin karyotype, such novel generation system can maintain the properties of the multipotential stem cell during long-term culture. In addition, the differentiative potential of the multipotentail stem cell is further verified by 15-generation suspension culture in an in-vitro embryoid body formation experiment and an in-vivo teratoma formation experiment.
Owner:INST OF ZOOLOGY CHINESE ACAD OF SCI
mRNA (messenger ribonucleic acid) quick-extraction kit
InactiveCN102978105AEasy to separate and extractRapid separation and extractionBioreactor/fermenter combinationsBiological substance pretreatmentsCelluloseTissue Lysis
The invention relates to an mRNA (messenger ribonucleic acid) quick-extraction kit and a preparation method thereof. The kit comprises a separation device for solid-liquid separation, wherein the separation device at least comprises a layer of cellulose solid-phase carrier. The kit also comprises at least one tube containing a cell or tissue lysis solution, at least one tube containing a biotinylated Oligo (dT) probe, at least one tube containing a combination buffer solution, and at least one tube containing an elution buffer solution. The kit is simple, quick and efficient to operate, and is suitable for separating and extracting mRNA in a biological sample. The invention is mainly characterized in that the separation device uses cellulose as the solid-phase carrier, a fusion protein CBD-SA is used as a biological bridging agent, and a biologically-specific coupling method is used instead of the traditional physical adsorption method and chemical bonding method to activate the cellulose solid-phase carrier, so that the activated solid-phase carrier can be efficiently combined with the biotinylated reagent to be used for separating protein or nucleic acid.
Owner:天津启仁医药科技有限公司
Kit for detecting ERCC1 mRNA (Excision Repair Cross Complement Group 1 Messenger Ribonucleic Acid) expression by using fluorescence quantitative PCR (Polymerase Chain Reaction) technology
InactiveCN102154475AHigh detection sensitivityImprove accuracyMicrobiological testing/measurementFluorescence/phosphorescenceExcision repair cross-complementingFluorescence
The invention relates to the field of biotechnology, discloses a fluorescence quantitative PCR (Polymerase Chain Reaction) diagnostic kit for rapidly detecting ERCC1 mRNA (Excision Repair Cross Complement Group 1 Messenger Ribonucleic Acid), and aims to provide a kit capable of rapidly, conveniently, sensitively and specifically detecting ERCC1 mRNA and application thereof. An expression status of a DNA (Deoxyribonucleic Acid) repair gene can be used as a relatively ideal predictor for a chemotherapeutic effect. Nucleotide excision repair (NER) is in close relation with platinum drug resistance, wherein the excision repair cross complement group 1 (ERCC1) is an important factor which plays a role in a repair process and causes platinum drug resistance; the ERCC1 mRNA level is detected by using higher-sensitivity and higher-specificity fluorescence quantitative PCR; and the specificity and the sensitivity of a detection result are obviously improved. The kit provides a completely new rapid and convenient gene diagnosis technology for use of platinum type chemotherapeutic medicaments of clinical malignant tumor patients.
Owner:SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
Goose-origin gene RIG-I (retinoic acid-inducible gene-I) with anti-Newcastle disease virus activities and application thereof
InactiveCN103173455AGrowth inhibitionCan exert anti-NDV effectFungiBacteriaEmbryoMessenger ribonucleic acid
Owner:SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
Method and kit for detecting high-risk HPV (human papillomavirus) E6/E7 mRNA (messenger ribonucleic acid) by ligase
InactiveCN105112564AEliminate the tedious steps of extractionReduce cumbersome stepsMicrobiological testing/measurementHuman papillomavirusComplementary deoxyribonucleic acid
The invention relates to a method and a kit for detecting high-risk HPV (human papillomavirus) E6 / E7 mRNA (messenger ribonucleic acid) by ligase. The method is characterized in that specific connection primers are connected into a cDNA (complementary deoxyribonucleic acid) fragment complementary with a target sequence of the HPV E6 / E7 mRNA under the action of the ligase, and the successfully connected cDNA fragment can be amplified by a pair of universal primers in a later fluorescent PCR (polymerase chain reaction) system, in other words, eighteen high-risk HPVs capable of causing cervical cancer can be detected in the same amplification system by the single fluorescent PCR technology, so that the problems, such as low amplification efficiency and sensitiveness, caused by excessive primers are solved effectively. The aim of HPV E6 / E7 mRNA detection is to screen out high-risk groups in HPV-infected people groups, so that the method has more significance in clinical diagnosis as compared with existing HPV genome L1 distinguishing type detection methods.
Owner:GUANGZHOU HEAS BIOTECH CO LTD
Gene expression amount comparing analysis method
InactiveCN1398988AHigh sensitivityGood quantitative effectMicrobiological testing/measurementDiseaseQuantitative determination
An analytical method for quantitative gene expression amount determination and comparison has great significance for disease-related gene screening and in clinical early diagnosis and development of specific medicine. The present invention utilizes quantitative characteristic of biological luminance process is sequencing to compare the gene expression amount of the same gene in different individuals or samples and to search disease-related gene. The specific steps includes: making mRNA with different sources by using a proper method and mixing in equal amount to form PCR substrate; PCR amplification with the primer corresponding to the gene of different sources and gene specific primer; and determining sequence by using bioluminescence analysis process with base variety to replace gene of different source.
Owner:周国华 +1
Common mussel C type lysozyme and preparation method thereof
InactiveCN102643787AStrong inhibitory activityMicroorganism based processesEnzymesComplementary deoxyribonucleic acidFeed additive
The invention discloses a common mussel C type lysozyme and a preparation method thereof. The amino acid sequence of the common mussel C type lysozyme is shown by a sequence table SEQ ID No.1. The preparation method of the common mussel C type lysozyme comprises the following steps of: extracting common mussel gross RNA (ribonucleic acid), purifying mRNA (Messenger ribonucleic acid), preparing a cDNA (complementary deoxyribonucleic acid) template solution, cloning gene, building recombinant plasmid, expressing expression plasmid and purifying protein to obtain the C type lysozyme. The common mussel C type lysozyme is a new C type lysozyme which has strong inhibitory activity on various gram positive and negative microorganisms, and has application value in the aspects of developing food preservatives, feed additive, etc.
Owner:NINGBO UNIV
Bicistronic mRNA (messenger ribonucleic acid) expression vector suitable for cells of mammals and application thereof
ActiveCN102392047AImprove stabilityHigh expressionVector-based foreign material introductionInteinBicistronic mrna
The invention discloses a bicistronic mRNA (messenger ribonucleic acid) expression vector which is suitable for cells of mammals and can realize high-efficient expression of exogenous genes and an application thereof. The bicistronic mRNA expression vector comprises connecting elements in the 5'-3' direction, namely (1) an open reading frame 1: containing the sequences of an early promoter / enhancer and an introne of cytomegalovirus, multiple clone sites and the sequence of a bovine growth hormone transcription terminator, which are sequentially arranged; (2) an open reading frame 2: containing the sequences of the early promoter / enhancer and the introne of the cytomegalovirus, the multiple clone sites and the sequences of the bovine growth hormone transcription terminator and a simian virus promoter, which are sequentially arranged; and (3) screening marker genes and the sequence of a simian virus 40 (SV40) terminator. The bicistronic mRNA expression vector has the high-efficient promoter, effective transcription termination signals, screening and gene amplification markers and two open reading frames, and can improve the expression efficiency of the exogenous genes and reduce the production cost.
Owner:优锐生物医药科技(深圳)有限公司
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