102 results about "Elisa kit" patented technology

The invention relates to the field of biological detection, in particular to a monoclonal antibody combination for detecting human IL-6 protein and application. The combination comprises monoclonal antibodies 3A10 and 2G1, and amino acid sequences of complementary determining regions of variable regions of light and heavy chains of the monoclonal antibodies 3A10 and 2G1 are respectively shown as SEQ ID NO.1-12. The invention defines a complete variable region sequence and a coding nucleotide sequence of the antibody. The combination specifically recognizes human IL-6 recombinant and natural proteins, and has no cross reaction with IL-11. A double-antibody sandwich ELISA kit constructed based on the combination takes 3A10 as a coating antibody and 2G1 as a labeled antibody, and has high sensitivity and high specificity. The problems that an existing reagent is insufficient in specificity and low in sensitivity are solved, a reliable core raw material is provided for IL-6 standardized detection, and the method is suitable for preparation of a kit, a test strip and an antibody chip.

The invention provides an ELISA (Enzyme Linked Immunosorbent Assay) kit for detecting an anti-MDA5 antibody and a preparation method of the ELISA kit. According to the kit, full-length human MDA5 protein (1025 amino acids) expressed by a cell-free protein expression system under the low-temperature condition of 22 DEG C is adopted as a coating antigen, and a disulfide bond enhancer E6820 is added into the system to promote correct folding of the protein. The kit comprises a microwell plate coated with a full-length MDA5 antigen, an enzyme-labeled anti-human IgG secondary antibody, a standard substance with the concentration range of 1-243 ng / mL, a chromogenic substrate and an optimized confining liquid system. The invention solves the problem of insufficient sensitivity caused by the use of fragment antigens in the prior art, and can be used for early diagnosis and illness monitoring of dermatomyositis-related interstitial lung diseases.

The invention belongs to the field of immunological detection, and provides a double-antibody sandwich ELISA kit for detecting the content of human beta-NGF. The enzyme-linked immunosorbent assay kit constructed by the invention is based on a double-antibody sandwich method, and is combined with a biotin-streptavidin signal amplification system and an enzyme developing amplification system, so that the detection sensitivity and specificity are greatly improved, and low-content samples can be accurately and quickly detected. The kit shows excellent stability and specificity and can be used for quantitatively detecting the content of beta-NGF in samples such as human serum, cell supernatant or tissue homogenate, so that an objective detection basis is provided for diagnosis, curative effect evaluation and pathogenesis research of related diseases.

This invention discloses a camel-derived CRP single-domain antibody, its preparation method, and its applications, relating to the biomedical field. The method involves enriching CRP antigen in alpaca leukocytes immunized with the antigen, followed by single-cell screening and sequencing. Through sequencing structural analysis, a recombinant plasmid of the camel-derived CRP antibody is constructed. This plasmid is cloned and transformed into eukaryotic cells to express related antibodies. Monoclonal antibodies with human CRP resistance are screened for expression. The VHH domain of the monoclonal antibody is cloned and expressed, and paired screening yields CRP-VHH monoclonal antibodies with antibody activity. This antibody can be used in ELISA kits, chemiluminescence kits, and latex kits, possessing the same functionality as existing kits. Furthermore, this antibody also has applications in the preparation of antitumor drugs.

The invention provides an anti-duck adenovirus 3 type monoclonal antibody, a hybridoma cell strain and application thereof, the anti-duck adenovirus 3 type monoclonal antibody is secreted by a hybridoma cell strain 2F12, the classification name of the hybridoma cell strain 2F12 is Hybridoma cell line 2F12, the hybridoma cell strain 2F12 is preserved in China Center for Type Culture Collection on November 13, 2025, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CGMCC NO. The preservation number of the strain is CCTCC (China Center For Type Culture Collection) NO: The blocking ELISA kit for detecting the duck adenovirus 3 antibody, which is prepared by utilizing the monoclonal antibody, is strong in specificity, high in sensitivity and good in repeatability, and has a very good application prospect in the aspects of epidemiological investigation of the duck adenovirus 3, vaccine immune efficacy evaluation and diagnosis of the duck adenovirus 3 disease.

The application belongs to the technical field of biology and particularly relates to an ELISA kit for detecting chicken astrovirus type II antibodies and a preparation method. The Cell-ELISA experiment is carried out by using LMH cells infected with chicken astrovirus type II to detect the chicken astrovirus type II antibodies. The method has no cross reactivity with positive serum of Newcastle disease virus, avian influenza virus, chicken infectious anemia virus, avian leukosis virus, Marek's virus and chicken infectious bursal virus, which indicates that the method has good specificity. The Cell-ELISA method is used to detect the chicken serum sample to be detected by using whole virus infection, and is assembled into a kit for measuring chicken astrovirus antibodies in serum. The kit can be used for serological diagnosis of chicken astrovirus type II infection, monitoring of the level of antibodies, epidemiological investigation and the like, and provides a novel and effective detection means for prevention and treatment of chicken astrovirus type II.

The application discloses a CRP camel-derived single-domain antibody and a preparation method and application thereof, relates to the field of biological medicines, and is characterized in that: white blood cells of a llama immunized with a CRP antigen are enriched, and then single-cell screening and sequencing are performed. Through sequencing structure analysis, a camel-derived CRP antibody recombinant plasmid is constructed, a related antibody is expressed in a eukaryotic cell through cloning transformation, a single antibody with human CRP resistance is screened, a VHH domain of the single antibody is cloned and expressed, and a single antibody with CRP-VHH antibody activity is obtained through pairing screening. The antibody can be applied to an ELISA kit, a chemiluminescence kit and a latex kit, has the same functions as existing kits, and still has the use in the preparation of an anti-tumor drug.

The invention provides an ELISA (Enzyme-Linked Immunosorbent Assay) detection kit for detecting rabbit hemorrhagic disease virus infection, and belongs to an in-vitro diagnostic reagent. The invention discloses application of RdRp protein in preparation of a detection kit for detecting rabbit hemorrhagic disease virus infection or distinguishing rabbit hemorrhagic disease virus infection and vaccine immunity. The RdRp protein has good conservative property in RHDV strains of GI.1 and GI.2 types at the same time, and the anti-RdRp protein antibody only exists in a rabbit hemorrhagic disease virus infected body and is not generated in a vaccine immunized body, so that the RdRp protein can be used for distinguishing rabbit hemorrhagic disease virus infection and vaccine immunization based on the specific binding property of the RdRp protein and the anti-RdRp protein antibody; the method greatly simplifies the diagnosis and identification operation, and has the advantages of good accuracy and repeatability, high detection sensitivity, strong detection specificity and great popularization and application value.

This invention relates to the field of biotechnology, and aims to provide a monoclonal antibody 7A12 for identifying genotype 2 classical swine fever, along with its preparation and application methods. This monoclonal antibody contains an Ig domain V of the antibody heavy chain. H CDR1, V H CDR2 and V H CDR3, and the Ig domain V of the antibody light chain L CDR1, V L CDR2 and V L CDR3; wherein, the V H CDR1, V H CDR2 and V H The amino acid sequences of CDR3 are shown in SEQ ID NO:1-3, respectively; the V L CDR1, V L CDR2 and V L The amino acid sequences of CDR3 are shown in SEQ ID NO:4-6. The antibody provided by this invention has advantages such as high titer, strong specificity, and high affinity; it also has high sensitivity and specificity, and shows no cross-reactivity with type 1 classical swine fever vaccine strains. Serologically, it can differentiate and diagnose infection and vaccine immunization with genotype 2 classical swine fever wild-type strains. Furthermore, it can be used to develop a blocking ELISA kit for identifying genotype 2 classical swine fever, effectively diagnosing and purifying genotype 2 classical swine fever virus.

The application discloses an antigen peptide targeting DRP1 protein Ser572 phosphorylation, an antibody and application thereof, and particularly relates to the technical field of biological medicines, and the antigen peptide has immunogenicity of targeting a DRP1 protein Ser572 phosphorylation site, wherein the amino acid sequence of the antigen peptide is RETKNVA{piSer}GGGGVGC, and {piSer} indicates that serine at the site is modified by phosphorylation. The antibody prepared by the application can stably recognize DRP1 Ser572 phosphorylation modification at a cell and animal level, can clearly reveal a molecular mechanism of the site phosphorylation in regulating DRP1 liquid-liquid phase separation and mediating myocardial mitochondrial damage, can support basic mechanism research of diseases such as sepsis, can be used for developing a specific ELISA kit and a pharmaceutical composition, fills a technical gap of DRP1 Ser572 phosphorylation related research and clinical transformation, and provides a brand-new technical support for diagnosis and treatment of cardiovascular diseases, sepsis and other major diseases.

The application provides an anti-human TL1A monoclonal antibody and a related kit and detection method thereof. The ELISA kit of the application can quantitatively detect the content of TL1A in various samples such as cell culture medium, is simple and convenient to operate, has high sensitivity, has good specificity, and can detect TL1A of different species, and has important significance for the research on the pathogenesis of TL1A related autoimmune diseases and drug models.

The application belongs to the field of immunological detection, and provides a double-antibody sandwich ELISA kit for detecting human Cyfra21-1 content. The anti-human Cyfra21-1 monoclonal antibody provided by the application can specifically bind to human Cyfra21-1, has no cross reaction with other similar proteins, and has high stability; based on the antibody, the double-antibody sandwich ELISA method for detecting human Cyfra21-1 has high sensitivity, specificity and accuracy, and can accurately and high-throughput detect the content of Cyfra21-1 in human serum or other serum analogs containing human Cyfra21-1.

The application provides a porcine epidemic diarrhea virus mutant strain and application thereof. The application obtains a porcine epidemic diarrhea virus mutant strain GX2506 from porcine intestinal tissue, and the strain is purified through passage, and the microorganism preservation number of the strain is CGMCC No. 47089. The isolated strain can stably proliferate on a passage cell, and typical cytopathic effect is generated. The porcine epidemic diarrhea virus mutant isolated strain has excellent immunogenicity; a vaccine prepared by using the isolated strain can induce piglets to generate high-level neutralizing antibodies; meanwhile, an ELISA kit prepared by using the isolated strain can better detect porcine epidemic diarrhea virus antibodies in serum.

This invention discloses anti-leptin monoclonal antibodies and their applications, relating to the field of biotechnology. This invention develops anti-leptin monoclonal antibodies 2A122H7 and 6B62E3, which can effectively recognize leptin. The ELISA kits prepared using these monoclonal antibodies 2A122H7 and 6B62E3 include indirect ELISA and double-antibody sandwich ELISA kits, exhibiting high specificity and sensitivity for the tested samples, and are convenient, efficient, and cost-effective. This invention provides effective technical support for advancing research on the regulatory mechanisms of economic traits such as feed intake, fat deposition, and reproduction in poultry.

The application provides a PLA2R monoclonal antibody and an ELISA kit comprising the antibody. Specifically, the application provides a monoclonal antibody which specifically binds to PLA2R, wherein the antibody only binds to non-reduced PLA2R antigen with a conformational epitope, does not bind to reduced PLA2R antigen, and does not bind to another antigen THSD7A of primary membranous nephropathy. An ELISA kit prepared based on the antibody can more efficiently and accurately detect primary membranous nephropathy than existing commercial kits.

The present application relates to the technical field of biology, in particular to a streptococcus pneumoniae urine antigen ELISA kit and a preparation method thereof.The solid-phase coating material of the kit is CRP, which is easy to prepare and obtain and low in cost;the reaction system only needs one antibody, greatly reducing the workload and labor cost of screening and preparation of paired antibodies;the antigen detection is fully automatic, greatly increasing the detection throughput and reducing the labor cost.

The present disclosure relates to the field of biotechnology, and particularly relates to detection of BXB1 integrase. The present disclosure establishes a method for detecting BXB1 integrase based on double-antibody sandwich ELISA technology, which has good sensitivity and specificity, and the method is simple to operate and can quickly realize accurate quantification of BXB1 integrase; in addition, the present disclosure also provides an ELISA kit for detecting BXB1 integrase. By using the BXB1 integrase detection method or kit provided by the present disclosure, accurate quantification of the residual amount of BXB1 integrase in various intermediate products, semi-finished products or finished products in the process of preparing recombinant biological products by the BXB1 integrase system can be realized, which is beneficial to the quality control of products by biopharmaceutical enterprises.

This invention provides a serum protein biomarker for early screening and diagnosis of pancreatic ductal adenocarcinoma. The serum protein biomarker is one or a combination of two or more autoantibodies against tumor-associated antigens HEXB, TXLNA, and SLAMF6. The invention also provides the application of reagents, kits, and analytical methods for detecting the serum protein biomarker. This invention screens for autoantibodies against pancreatic ductal adenocarcinoma-associated antigens. By measuring the expression levels of these indicators in the serum of patients with pancreatic ductal adenocarcinoma, healthy controls, and controls with benign pancreatic diseases, the screened anti-HEXB, anti-TXLNA, and anti-SLAMF6 autoantibodies can distinguish between pancreatic ductal adenocarcinoma, healthy controls, and benign pancreatic diseases. Furthermore, this invention designs and optimizes combinations of tumor-associated antigens for the diagnosis of pancreatic ductal adenocarcinoma. Applying the optimized antigens to an ELISA kit and detecting serum anti-TAA autoantibodies using an indirect enzyme-linked immunosorbent assay (ELISA) can accurately identify patients with pancreatic ductal adenocarcinoma, healthy controls, and patients with benign pancreatic diseases.

This invention discloses an accurate and comparable method for detecting low-concentration intracellular and extracellular *Streptocytoxin*, belonging to the field of environmental monitoring technology. The method includes the following steps: S1. Calibration of *Streptocytoxin* standards and construction of cross-platform standard curves; S2. Separation and pretreatment of intracellular and extracellular *Streptocytoxin* in water samples; S3. Solid-phase extraction to concentrate and enrich *Streptocytoxin*; S4. LC-MS / MS detection and quantitative calculation. This invention binds the LC-MS / MS results to the built-in standards of widely commercially available and validated ELISA kits, using the kit's built-in standards as a benchmark for standard calibration. This fundamentally avoids systematic errors between standards from different brands and enhances the correlation between ELISA and LC-MS / MS results, providing reliable methodological support for integrating long-term monitoring data from multiple reservoirs, comparing pollution levels across regions, and reanalyzing historical monitoring data.

The application discloses a hybridoma cell strain secreting monoclonal antibody PVY-5, the monoclonal antibody and application, the hybridoma cell strain 4B4D8C3 can secrete anti-potato Y virus monoclonal antibody, the preservation number of the hybridoma cell strain is CCTCC NO: C2022282, the monoclonal antibody PVY-5 has specific immunoreaction with the coat protein of potato Y virus, the heavy chain variable region amino acid sequence of the monoclonal antibody PVY-5 is as shown in SEQ ID NO.6, the light chain variable region amino acid sequence is as shown in SEQ ID NO.7, the sensitivity reaches 1:5120 times dilution by using direct ELISA method detection to the leaf of potato Y virus disease infection, the monoclonal antibody PVY-5 can also be used to prepare ELISA kit, or colloidal gold kit, or nano analog enzyme kit, and the potato Y virus disease can be detected quickly, sensitively, economically and accurately.

The invention belongs to the field of biology, and discloses a PDCoV recombinant RBD protein and an IgA Elisa kit thereof, and the amino acid sequence of the recombinant RBD protein is as shown in SEQ ID NO. 1. The recombinant RBD protein is used for an indirect ELISA kit, can cover IgA response in the early stage of infection, can accurately recognize a PDCoV specific IgA antibody in a breast milk sample, and cannot cause false positive due to infection of other common pathogens in a pig herd; the indirect ELISA kit is good in stability (the intra-batch variation coefficient is 1.848%-6.062%, and the inter-batch variation coefficient is 4.504%-7.790%), the coincidence rate of the indirect ELISA kit and an immunofluorescence experiment reaches 93.26%, the repeatability and the coincidence rate are high, and the indirect ELISA kit has the advantages of simplicity and convenience in operation, short time consumption and large detection flux of an ELISA method, and is suitable for large-scale screening of clinical pig milk samples.

The invention discloses a nano antibody for detecting glycinin, an ELISA kit and application thereof, and belongs to the technical field of glycinin detection. Wherein the nano antibody comprises a nano antibody 1 and a nano antibody 2 which can be respectively used as a coating antibody and a detection antibody (biotin label) in the ELISA kit; the ELISA kit can also comprise reagents such as enzyme-labeled streptavidin, a color developing solution and the like, and the quantitative analysis of the glycinin in a to-be-detected sample is realized by specifically utilizing a sandwich ELISA method and a color developing reaction. According to the scheme, the specificity is high, the detection limit is low, glycinin residues in products such as fermented soybeans, enzymolysis soybeans, soybean meal and feed can be accurately and sensitively detected, meanwhile, the defects that in traditional instrument detection, sample pretreatment is complex, consumed time is long and the like are overcome, and the detection cost is greatly saved.

The invention relates to an ELISA (enzyme-linked immuno sorbent assay) kit for quantitative detection of humanized exosomes and a use method of the ELISA kit, the ELISA kit comprises an ELISA plate coated with a capture antibody, a detection antibody solution, an enzyme-labeled streptavidin solution for catalyzing substrate color development and a luminous substrate solution, the capture antibody is an anti-CD81 antibody, and the detection antibody solution is an enzyme-labeled streptavidin solution for catalyzing substrate color development. According to the present invention, the detection antibodies comprise the biotin-labeled anti-CD9 antibody and the biotin-labeled anti-CD63 antibody, the specific capture of the humanized exosome is performed through the anti-CD81 antibody coated on the pore plate, the detection is performed through the anti-CD9 antibody and the anti-CD63 antibody, and the quantification of the humanized exosome is achieved through the enzyme catalysis chemiluminescence principle, by optimizing the elisa plate and each reagent in the kit and adjusting the use method, the humanized exosome is accurately quantified, and the detection result is not interfered by protein impurities, so that the purpose of rapid and accurate quantification is achieved.

This invention discloses a bivalent anti-Vibrio parahaemolyticus nanobody, its preparation method, a self-sandwich ELISA kit, and a detection method. The aim is to provide a bivalent anti-Vibrio parahaemolyticus nanobody, kit, and detection method with high accuracy, specificity, and sensitivity for detecting Vibrio parahaemolyticus. The amino acid sequence of the bivalent anti-Vibrio parahaemolyticus nanobody is shown in SEQ ID NO.7. This invention belongs to the field of biodetection technology.

The invention provides an anti-HPV45L1 protein monoclonal antibody as well as a preparation method and application thereof, and belongs to the technical field of biology. The monoclonal antibodies 15D4 and 17F9 provided by the invention can be specifically combined with the 45 subtype of HPV, have the characteristics of neutralizing the HPV59 pseudovirus and blocking the infection of the HPV59 pseudovirus, and can be used for detecting the HPV45 antigen. Meanwhile, the epitopes recognized by the two monoclonal antibodies are epitopes targeted by dominant neutralizing antibodies in serum after vaccine immunization, so that the epitopes can be used for antigen detection and can also be directly used for evaluating the neutralizing antibody generation capability induced by the HPV vaccine. The invention further provides a double-antibody sandwich ELISA kit, the kit can specifically recognize and quantify the HPV45LI protein with complete conformation and immunogenicity in a sample, the kit can be stably produced in batch, rapid evaluation of the in-vitro activity of the HPV vaccine is achieved, and the kit has a good application prospect.

This invention discloses a monoclonal antibody MM03H against human Mycoplasma pneumoniae CARDS TX, prepared from a hybridoma cell line deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 21420. Currently, there are no commercially available anti-CARDS toxin antibodies, and even fewer ELISA kits targeting the CARDS TX antigen. This invention, for the first time, discloses a monoclonal antibody MM03H against human Mycoplasma pneumoniae CARDS TX protein. Upon binding to the human Mycoplasma pneumoniae CARDS TX protein, it can inhibit the entry of the human Mycoplasma pneumoniae CARDS TX protein across the cell membrane into the cytoplasm.

The invention relates to the technical field of protein detection, and particularly provides a double-antibody sandwich ELISA kit for detecting H5 subtype avian influenza virus M1 protein. The inactivated H5 subtype avian influenza virus subjected to sucrose density gradient centrifugal concentration and purification is used as an immunogen to immunize a BALB / c mouse, the H5 subtype avian influenza virus M1 protein is used for screening to obtain a detection antibody, and the detection antibody is prepared after an SPF chicken is immunized. On the basis, a double-antibody sandwich ELISA kit for detecting the H5 subtype avian influenza virus M1 protein is researched and developed. The technology can be used for quantitative detection of H5 subtype avian influenza M1 protein and quality control of avian influenza subunit products, and ensures stable product quality; and the method can also be used for detecting the expression characteristics of the recombinant avian influenza baculovirus seed protein.

The application discloses a double-antibody sandwich ELISA kit for detecting the content of CTHRC1 in blood and a detection method and application thereof, and belongs to the field of molecular biological techniques. In view of the problem that it is difficult to identify early acute aortic dissection at present, a pair of capture antibodies and detection antibodies capable of recognizing different epitopes of CTHRC1 protein antigens are used in the application to assemble a double-antibody sandwich ELISA kit for detecting the content of CTHRC1 in blood. The double-antibody sandwich ELISA kit has high specificity and sensitivity for CTHRC1 protein in serum, can quantitatively detect the CTHRC1 protein in serum, and thus can accurately identify early acute aortic dissection, thereby avoiding misdiagnosis.

The application discloses an ELISA kit for detecting anti-chromatin antibodies. The kit contains purified chromatin monomers with stripped H1 histone components in a natural structure. The application also discloses a detection method for the ELISA kit. The chromatin structure in the eukaryotic cell nucleus is complex, and is a natural complex composed of 40% DNA, 40% histone, 20% non-histone, a small amount of RNA and other macromolecules. The purification technology is high in requirement, and the price of the natural purified chromatin monomers is high. The application purifies the chromatin monomers with stripped H1 histone components according to the structure of the natural chromatin, retains the effective antigen components, and greatly reduces the cost. The kit can be used for detecting the anti-chromatin antibodies, fills the blank of the domestic anti-chromatin antibody detection method, and has the characteristics of large detection flux, accurate result, high sensitivity and simple operation.

The invention discloses application of serum alpha-non-red blood cell ghost protein 1 in preparation of drugs for diagnosing heart light chain amyloidosis diseases, and belongs to the technical field of biological medicines. The serum SPTAN1 protein is used for preparing the medicine for diagnosing the heart light-chain amyloidosis disease, the medicine is limited to be an enzyme linked immunosorbent assay kit, and the kit comprises an antibody of the SPTAN1 protein and further comprises a pre-coated plate, a standard substance, a coating buffer solution, a sample diluent, a confining liquid, horse radish peroxidase, an eluent and a color developing agent. The concentration of the SPTAN1 protein in the serum is measured through an enzyme linked immunosorbent assay kit, and early diagnosis of the heart AL amyloidosis disease is carried out. The invention provides a novel, efficient and sensitive biomarker, namely the serum SPTAN1 protein, early diagnosis of heart AL amyloidosis is realized by detecting the concentration of the SPTAN1 protein in serum, and the serum SPTAN1 protein has an important clinical application prospect.