352 results about "Elisa kit" patented technology

The present invention belongs to the field of biological detection technology, specifically relating to a monoclonal antibody combination for detecting HPV18 E7 protein and its application. This combination consists of monoclonal antibodies 5F8 and 6G6, wherein the heavy and light chain variable regions of 5F8 and 6G6, respectively, contain specific complementarity-determining region sequences (as shown in SEQ ID NOs. 1 to 12), conferring high specificity for HPV18 E7 protein. Verification using a double-antibody sandwich ELISA and a biotin-avidin amplification system demonstrated that this antibody combination specifically recognizes HPV18 E7 protein, exhibits no cross-reactivity with other high-risk HPV oncoproteins, and exhibits a detection sensitivity of 1 ng / mL. This combination can be used to construct detection systems such as ELISA kits and immunochromatographic test strips, suitable for rapid screening of HPV18 E7 protein in cervical exfoliated cell samples.

The invention belongs to the technical field of biological medicines, and particularly relates to application of secretory lactase-like protein (LCTL) as a diagnostic marker in preparation of a diagnostic kit for chronic kidney diseases. According to the present invention, the secretory form of the LCTL is found for the first time, the secretory LCTL content in the human serum is detected by using the existing commercial ELISA kit for the first time, and the further research finds that the secretory LCTL content in the serum of the chronic kidney disease patient is significantly reduced compared with the normal person; and the content of the LCTL in the serum is obviously related to the renal function impairment degree and proteinuria degree of the patient, so that the application of the secretory LCTL in preparing the biomarker for diagnosing the chronic kidney disease is provided for the first time. The invention provides a new method for chronic kidney disease diagnosis, and has important clinical significance and commercial value.

The invention relates to the technical field of biology, and aims to provide a monoclonal antibody 7A12 for identifying gene type 2 swine fever as well as a preparation method and an application method of the monoclonal antibody 7A12. The monoclonal antibody comprises Ig structural domains VH CDR1, VH CDR2 and VH CDR3 of a heavy chain of the antibody, and Ig structural domains VL CDR1, VL CDR2 and VL CDR3 of a light chain of the antibody, wherein the amino acid sequences of the VH CDR1, the VH CDR2 and the VH CDR3 are respectively as shown in SEQ ID NO: 1 to SEQ ID NO: 3; the amino acid sequences of the VL CDR1, the VL CDR2 and the VL CDR3 are respectively as shown in SEQ ID NO: 4 to SEQ ID NO: 6. The antibody provided by the invention has the advantages of high titer, strong specificity, high affinity and the like; the kit has the advantages of high sensitivity and strong specificity, has no cross reaction with the type 1 swine fever vaccine strain, and can identify and diagnose swine fever gene type 2 wild strain infection and vaccine immunity in serology; the method can be further used for establishing a blocking ELISA kit for identifying the gene type 2 hog cholera, and can effectively diagnose and purify the gene type 2 hog cholera virus.

The present invention discloses a porcine Seneca virus neutralization liquid phase blocking ELISA kit, which comprises an effective amount of porcine Seneca virus antigen, an effective amount of porcine Seneca virus single domain antibody 1, an effective amount of porcine Seneca virus single domain antibody 2, and a matching detection reagent. The porcine Seneca virus antigen is porcine Seneca virus particles purified by sucrose density gradient centrifugation, and the particle size of the purified porcine Seneca virus particles is 20 to 30 nm. The amino acid sequence of the heavy chain variable region of the porcine Seneca virus single domain antibody 1 is shown in SEQ ID NO.1. The amino acid sequence of the heavy chain variable region of the porcine Seneca virus single domain antibody 2 is shown in SEQ ID NO.2. The kit of the present invention has the advantages of high efficiency, simplicity, sensitivity, stability, etc., has a good correlation with the traditional diagnostic method virus neutralization experiment, and is suitable for a wide range of applications.

The invention relates to the field of biological detection, in particular to a monoclonal antibody combination for detecting human IL-6 protein and application. The combination comprises monoclonal antibodies 3A10 and 2G1, and amino acid sequences of complementary determining regions of variable regions of light and heavy chains of the monoclonal antibodies 3A10 and 2G1 are respectively shown as SEQ ID NO.1-12. The invention defines a complete variable region sequence and a coding nucleotide sequence of the antibody. The combination specifically recognizes human IL-6 recombinant and natural proteins, and has no cross reaction with IL-11. A double-antibody sandwich ELISA kit constructed based on the combination takes 3A10 as a coating antibody and 2G1 as a labeled antibody, and has high sensitivity and high specificity. The problems that an existing reagent is insufficient in specificity and low in sensitivity are solved, a reliable core raw material is provided for IL-6 standardized detection, and the method is suitable for preparation of a kit, a test strip and an antibody chip.

The invention discloses an enzyme-linked immunosorbent assay kit for detecting the content of soluble IL-6 (interleukin-6) protein. The enzyme-linked immunosorbent assay kit comprises two anti-human IL-6 monoclonal antibodies, namely, IL-6-1C7 and IL-6-2E11. The amino acid sequence of a heavy chain variable region of the IL-6-1C7 monoclonal antibody is SEQ ID NO.1, and the amino acid sequence of a light chain variable region of the IL-6-1C7 monoclonal antibody is SEQ ID NO.2; the amino acid sequence of a heavy chain variable region of the IL-6-2E11 is SEQ ID NO.3, and the amino acid sequence of a light chain variable region of the IL-6-2E11 is SEQ ID NO.4. The enzyme linked immunosorbent assay kit provided by the invention can effectively detect the content of soluble IL-6, and detection by using the kit finds that serum of healthy people contains soluble IL-6 protein with a certain basic level, the content of soluble IL-6 in serum of patients with inflammatory and autoimmune diseases and the like is obviously increased and is related to clinical staging and pathology of the patients, so that the content of soluble IL-6 in serum of patients with inflammatory and autoimmune diseases is obviously reduced. The detection of the content of the soluble IL-6 in the body fluid of the inflammatory patient has clinical value.

The invention provides a hybrid creatine kinase isoenzyme double-antibody sandwich ELISA kit and a preparation method thereof, and belongs to the technical field of CK-MB antibody and creatine kinase isoenzyme quantitative detection. The process comprises the following steps: respectively immunizing Japanese big-ear rabbits and mice by using CK-MB heterodimer protein as an immunogen, screening positive monoclonal cells by adopting a Beacon single-cell screening platform, and preparing a rabbit (mouse) anti-human CK-MB gene engineering antibody; the double-antibody sandwich ELISA detection kit is established by further screening optimal reaction conditions. The detection method disclosed by the invention has excellent performance in the aspects of intra-batch and inter-batch repeatability, sensitivity and specificity, and is suitable for CK-MB detection in clinical and scientific research.

The invention provides an ELISA (Enzyme Linked Immunosorbent Assay) kit for detecting an anti-MDA5 antibody and a preparation method of the ELISA kit. According to the kit, full-length human MDA5 protein (1025 amino acids) expressed by a cell-free protein expression system under the low-temperature condition of 22 DEG C is adopted as a coating antigen, and a disulfide bond enhancer E6820 is added into the system to promote correct folding of the protein. The kit comprises a microwell plate coated with a full-length MDA5 antigen, an enzyme-labeled anti-human IgG secondary antibody, a standard substance with the concentration range of 1-243 ng / mL, a chromogenic substrate and an optimized confining liquid system. The invention solves the problem of insufficient sensitivity caused by the use of fragment antigens in the prior art, and can be used for early diagnosis and illness monitoring of dermatomyositis-related interstitial lung diseases.

The invention belongs to the field of immunological detection, and provides a double-antibody sandwich ELISA kit for detecting the content of human beta-NGF. The enzyme-linked immunosorbent assay kit constructed by the invention is based on a double-antibody sandwich method, and is combined with a biotin-streptavidin signal amplification system and an enzyme developing amplification system, so that the detection sensitivity and specificity are greatly improved, and low-content samples can be accurately and quickly detected. The kit shows excellent stability and specificity and can be used for quantitatively detecting the content of beta-NGF in samples such as human serum, cell supernatant or tissue homogenate, so that an objective detection basis is provided for diagnosis, curative effect evaluation and pathogenesis research of related diseases.

The invention discloses a human IL-13 antibody, an ELISA kit containing the human IL-13 antibody and a detection method of the human IL-13 antibody. Specifically, the ELISA kit provided by the invention comprises a pair of anti-human IL-13 monoclonal antibodies which are respectively used as a coating antibody and a detection antibody in a double-antibody sandwich ELISA method. The kit can be used for successfully completing quantitative detection of human IL-13, has relatively high detection sensitivity and good detection specificity, can be used for detecting a plurality of species of IL-13, and has good development and application prospects.

The invention discloses an enzyme linked immunosorbent assay kit for detecting a plasticizer in edible oil and application of the enzyme linked immunosorbent assay kit, and belongs to the technical field of enzyme linked immunosorbent assay. The enzyme linked immunosorbent assay kit comprises an elisa plate coated with a plasticizer coupling antigen, a plasticizer standard substance, an enzyme conjugate working solution, a plasticizer specific antibody working solution, a purification tube, a purification reagent, a standard substance diluent, a sample diluent, a substrate solution, a stop solution and a 10 * concentrated washing solution. The standard substance diluent provided by the invention is used for diluting a plasticizer standard substance at different temperatures to obtain a plasticizer standard solution, and the obtained plasticizer standard solution has basically consistent inhibition ratio, so that the stability of the kit is remarkably enhanced; meanwhile, the standard diluent and other components in the kit have a synergistic effect, so that the enzyme linked immunosorbent assay kit has the advantages of good specificity, high sensitivity, good accuracy and high stability when being used for detecting the plasticizer in the edible oil; therefore, the enzyme linked immunosorbent assay kit has a good application prospect in the detection of the plasticizer in the edible oil.

The application provides an AFP detection kit and application thereof, relates to the technical field of tumor marker detection, and the kit is a double-antibody sandwich ELISA kit. The kit contains two AFP monoclonal antibodies, AFPH8 and AFPE2. The amino acid sequences of the CDR of the heavy chain of AFPH8 are shown in SEQ ID NO. 4-6, the amino acid sequences of the CDR of the light chain of AFPH8 are shown in SEQ ID NO. 7-9, the amino acid sequences of the CDR of the heavy chain of AFPE2 are shown in SEQ ID NO. 12-14, and the amino acid sequences of the CDR of the light chain of AFPE2 are shown in SEQ ID NO. 15-17. Experiments show that the kit has high sensitivity and can detect slightly elevated AFP in initial serum.

The invention discloses an indirect ELISA (Enzyme-Linked Immunosorbent Assay) detection kit for distinguishing host infected Listeria monocytogenes and / or Listeria ivanovii. The coating antigens are respectively a recombinant listeria monocytogenes surface protein ActA, a serotype 4h listeria monocytogenes and listeria ivanovii common outer membrane protein SmcL and a listeria ivanovii specific outer membrane protein I-inlH, indirect ELISA methods are respectively established based on the three antigens, the three detection methods are combined to detect the content of the listeria monocytogenes, and the content of the listeria ivanovii can be detected. The levels of the ActA antibody, the SmcL antibody and the I-inlH antibody in a clinical serum sample can be effectively monitored, and the detection on the listeria monocytogenes caused by the listeria monocytogenes and / or the listeria ivanovii is realized. The kit is relatively high in sensitivity, strong in specificity and good in stability, and an effective method is provided for epidemiological investigation and diagnosis of listeria monocytogenes.

This invention discloses a camel-derived CRP single-domain antibody, its preparation method, and its applications, relating to the biomedical field. The method involves enriching CRP antigen in alpaca leukocytes immunized with the antigen, followed by single-cell screening and sequencing. Through sequencing structural analysis, a recombinant plasmid of the camel-derived CRP antibody is constructed. This plasmid is cloned and transformed into eukaryotic cells to express related antibodies. Monoclonal antibodies with human CRP resistance are screened for expression. The VHH domain of the monoclonal antibody is cloned and expressed, and paired screening yields CRP-VHH monoclonal antibodies with antibody activity. This antibody can be used in ELISA kits, chemiluminescence kits, and latex kits, possessing the same functionality as existing kits. Furthermore, this antibody also has applications in the preparation of antitumor drugs.

The utility model provides an ELISA (Enzyme Linked Immunosorbent Assay) kit which comprises a kit body and a kit cover, a partition plate is fixedly connected to the top of the kit body, a drawer is embedded and inserted into the kit body, the top of the partition plate is fixedly connected with a supporting plate through a spring, an insertion plate is fixedly connected to the bottom of the supporting plate, the insertion plate and a limiting plate are correspondingly distributed, and the kit cover is attached to a placing mechanism. And the placing mechanism is fixedly mounted on the top surface of the supporting plate. The kit body is used for placing elisa plates and other reagent bottles, separate storage can be achieved, meanwhile, the reagent bottles are stored and fixed, the stability in the carrying process is ensured, the damage problem is avoided, the elisa plates can be conveniently taken and used by arranging the drawer, the elisa plates can be conveniently stored by utilizing the supporting plate and the placing plate, and the kit is convenient to use. According to the ELISA kit, the drawer can be unlocked by opening the kit cover, and the pressing plate on the kit cover can be used for pressing and storing the ELISA plate, so that the stability of the ELISA plate in the carrying process is ensured, various articles are convenient to take and place, and the use convenience of the ELISA kit is improved.

The invention provides an anti-human TL1A monoclonal antibody as well as a related kit and a detection method thereof. The ELISA kit provided by the invention can quantitatively detect the content of TL1A in various samples such as cell culture media, is simple and convenient to operate, has high sensitivity and good specificity, can detect TL1A of different species, and has important significance in research on pathogenesis of TL1A-related autoimmune diseases, drug models and the like.

The invention discloses a porcine delta-coronavirus double-antibody sandwich ELISA (enzyme-linked immuno sorbent assay) detection kit based on an S protein monoclonal antibody as well as a use method and application of the porcine delta-coronavirus double-antibody sandwich ELISA detection kit. A PDCoV S protein is expressed by adopting an ExpiCHO expression system, then the purified S protein is used as an immunogen to immunize a mouse, and the monoclonal antibody is obtained. And then, respectively using the monoclonal antibody 3D7 and the MAb 9G4 labeled by the HRP as capture and detection antibodies, and establishing the double-antibody sandwich ELISA kit. The kit has good sensitivity, the detection limit of purified S protein is 0.12 ng / mL, and the detection limit of PDCoV is 1.96 * 10 < 3 > copies / [mu] L. Specificity analysis shows that the kit has no cross reactivity with other porcine intestinal coronaviruses PEDV, TGEV and PoRV with clinical symptoms similar to PDCoV, the consistency with an RT-PCR (reverse transcription-polymerase chain reaction) method reaches 91.03%, and the Kappa value is 0.814, so that the reliability of the kit for clinical sample detection is shown. In addition, the kit can also quantitatively evaluate the content of S protein in PDCoV inactivated vaccines and subunit vaccines, and provides a powerful tool for evaluating the vaccines.

The invention provides an anti-duck adenovirus 3 type monoclonal antibody, a hybridoma cell strain and application thereof, the anti-duck adenovirus 3 type monoclonal antibody is secreted by a hybridoma cell strain 2F12, the classification name of the hybridoma cell strain 2F12 is Hybridoma cell line 2F12, the hybridoma cell strain 2F12 is preserved in China Center for Type Culture Collection on November 13, 2025, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CGMCC NO. The preservation number of the strain is CCTCC (China Center For Type Culture Collection) NO: The blocking ELISA kit for detecting the duck adenovirus 3 antibody, which is prepared by utilizing the monoclonal antibody, is strong in specificity, high in sensitivity and good in repeatability, and has a very good application prospect in the aspects of epidemiological investigation of the duck adenovirus 3, vaccine immune efficacy evaluation and diagnosis of the duck adenovirus 3 disease.

The invention relates to the technical field of biochemical detection, in particular to an enzyme linked immunosorbent assay kit for detecting diisononyl phthalate and application of the enzyme linked immunosorbent assay kit. The invention relates to an enzyme linked immunosorbent assay kit for detecting diisononyl phthalate. The enzyme linked immunosorbent assay kit comprises the following components: an elisa plate coated with a coating antigen, a diisononyl phthalate specific antibody working solution, an enzyme marker working solution, a diisononyl phthalate standard substance solution, a substrate developing solution, a stopping solution, a washing solution, a sample diluting solution and a purifying agent. According to the invention, the content of diisononyl phthalate in an edible oil sample is qualitatively or quantitatively detected by mainly adopting an indirect competitive ELISA method; the sample pretreatment requirement is low, the sample pretreatment process is simple, and a large batch of edible oil samples can be rapidly detected at the same time; the main reagent is provided in the form of a working solution, and the detection method is convenient and easy to implement and has the characteristics of high specificity, high sensitivity, high precision, high accuracy and the like.

The invention discloses a double-antibody sandwich ELISA (Enzyme Linked Immunosorbent Assay) kit capable of identifying highly pathogenic and non-pathogenic serum type 4 fowl adenoviruses and application thereof. The kit comprises an anti-serum type 4 fowl adenovirus monoclonal antibody 6F11 and an anti-highly pathogenic serum type 4 fowl adenovirus monoclonal antibody 2C5. The kit prepared by the invention can accurately identify and diagnose HHS caused by highly pathogenic serum type 4 fowl adenovirus, eliminates interference of non-pathogenic serum type 4 fowl adenovirus on HHS diagnosis in clinical detection, has the advantages of strong specificity, high sensitivity, good repeatability, low cost, simplicity and convenience in operation and the like, can realize rapid detection of a large batch of samples, and has a wide application prospect. And a technical support is provided for prevention and control of HP-FAdV-4.

The invention provides a bovine akabane disease virus recombinant N protein antigen, a preparation method and an ELISA kit, relates to the field of biotechnology detection, and solves the problems that in the prior art, a competitive ELISA technical means is generally adopted in an ELISA detection method, the preparation cost is high, the price of a commercialized kit is high, and the detection cost is low. The development of beef cattle breeding enterprises in China is not facilitated. The amino acid sequence of the bovine akabane disease virus recombinant N protein antigen is as shown in SEQ ID No.2. The bovine akabane disease virus recombinant N protein antigen obtained in the invention can be specifically combined with an antibody of bovine akabane disease virus through verification, and can be used as a candidate antigen for bovine akabane disease virus detection; the ELISA detection kit developed by the invention can be used for detecting the bovine akabane disease virus antibody, and has the advantages of strong specificity, high sensitivity, good repeatability, low cost, large-scale detection and the like.

The invention relates to the field of biological immunity and pharmaceutical preparations, and particularly discloses a human growth hormone (GH) freeze-drying protective agent. The invention further discloses a freeze-drying preparation prepared from the freeze-drying protective agent. The freeze-drying preparation mainly comprises the following components in parts by weight: 10-100 mM of a buffer solution, 1-30 parts of a protective agent, a stabilizer and an excipient, 0.01-1.5 parts of a surfactant, 0.1-0.8 part of an antioxidant and 0.05-3 parts of a preservative. The invention provides a specific preparation method of the freeze-dried preparation, the preparation has the advantages of long preservation period, short redissolution time compared with a common reagent, good form and good structural stability after freeze-drying, provides favorable protection conditions for preparation of ELISA standard substances and internal control substances, is beneficial to maintenance of the stability of proteins, and has good application prospects. The specificity and the sensitivity of the ELISA kit are improved.

The invention relates to the field of biology, and discloses an enzyme linked immunosorbent assay kit for detecting human metapneumovirus antigens and a quantitative detection method.The enzyme linked immunosorbent assay kit for detecting the human metapneumovirus antigens comprises a capture antibody which is a human metapneumovirus specific antibody HMPV01 or an antigen binding fragment thereof and comprises an HMPV01 antibody light chain amino acid sequence SEQ ID NO: 1 and an HMPV01 antibody heavy chain amino acid sequence SEQ ID NO: 2; the recognition antibody is a human metapneumovirus specific enzyme-labeled antibody HMPV02-HRP or an antigen binding fragment of the human metapneumovirus specific enzyme-labeled antibody HMPV02-HRP. A capture antibody HMPV01 and a recognition antibody HMPV02-HRP with specific amino acid sequences are screened, the antibody is efficiently prepared by using a mammalian cell expression system, and an optimized detection method is combined, so that the binding efficiency of the antibody and the antigen is remarkably improved, and the kit can detect the low-concentration human metapneumovirus antigen.

The invention provides a porcine epidemic diarrhea virus G2c subtype strain and application thereof. According to the invention, the porcine epidemic diarrhea virus G2c subtype HN2501 strain is separated from porcine intestinal tissues and is subjected to passage purification, and the microbial preservation number of the porcine epidemic diarrhea virus G2c subtype HN2501 strain is CGMCC (China General Microbiological Culture Collection Center) No.46417. The isolated strain can stably proliferate on passage cells to generate typical cytopathy. The G2c subtype isolated strain of the porcine epidemic diarrhea virus has excellent immunogenicity; the vaccine prepared from the isolated strain can induce piglets to generate high-level neutralizing antibodies; meanwhile, the ELISA kit prepared from the isolated strain can be used for well detecting the porcine epidemic diarrhea virus antibody in serum.

The invention belongs to the technical field of biological detection, and particularly relates to a monoclonal antibody 1E12 for specific detection of AAV6 and application of the monoclonal antibody 1E12. According to the monoclonal antibody 1E12 provided by the invention, a heavy chain variable region and a light chain variable region respectively comprise three complementary determining regions (CDR) corresponding to SEQ ID NO.1-3 and SEQ ID NO.4-6. The CDRs endow the antibody with high specificity and affinity to AAV6, the antibody can accurately recognize AAV6 virus without cross reaction with other serotypes, and the detection sensitivity reaches 4 * 10 < 6 > vg / ml level. The antibody is suitable for development of multiple detection tools such as a double-antibody sandwich ELISA kit, a test strip and an antibody chip, especially has excellent performance in a biotin-avidin amplification system, can be used for virus identification, titer determination and clinical dose evaluation of AAV6, and provides an effective means for standardized and high-throughput detection.

The invention relates to the technical field of biological detection, and particularly discloses an ELISA kit for detecting a neutralizing antibody of a severe fever with thrombocytopenia syndrome virus and an application method of the ELISA kit. The ELISA kit comprises an ELISA plate on which SFTSV receptor LRP1 protein is fixed, and an enzyme-labeled SFTSV Gn-8M, wherein the SFTSV receptor LRP1 protein is fixed on the ELISA plate; the SFTSV Gn-8M is an SFTSV membrane protein Gn mutant designed by the invention, and can be specifically combined with an SFTSV receptor LRP1 protein on an elisa plate, and compared with a standard virus strain Gn protein, the self-designed SFTSV Gn-8M has amino acid mutation of eight sites, has stronger combining capacity with a host cell receptor, and can be used for detecting the SFTSV. The kit disclosed by the invention has the characteristics of high safety, high efficiency, high flux, high specificity, high sensitivity, high stability and the like, and is suitable for large-scale rapid screening of the neutralizing antibody of the severe fever with thrombocytopenia syndrome virus.

The invention relates to the technical field of biomedicine, and particularly discloses an ARHGAP42 marker for pancreatic cancer diagnosis and prognosis evaluation and application of the ARHGAP42 marker. Experiments prove that the ARHGAP42 is remarkably and highly expressed in pancreatic cancer tissues, and the expression level of the ARHGAP42 is remarkably related to clinical histological grading, lifetime and immunity of a patient. The invention provides application of a reagent for detecting the expression level of ARHGAP42 in preparation of a pancreatic cancer diagnosis or prognosis evaluation tool, including but not limited to qPCR primers, antibodies, ELISA kits or gene chips. In addition, the invention also covers the application of the drug for targeted inhibition of ARHGAP42 in treatment of pancreatic cancer.

The application belongs to the technical field of biology and particularly relates to an ELISA kit for detecting chicken astrovirus type II antibodies and a preparation method. The Cell-ELISA experiment is carried out by using LMH cells infected with chicken astrovirus type II to detect the chicken astrovirus type II antibodies. The method has no cross reactivity with positive serum of Newcastle disease virus, avian influenza virus, chicken infectious anemia virus, avian leukosis virus, Marek's virus and chicken infectious bursal virus, which indicates that the method has good specificity. The Cell-ELISA method is used to detect the chicken serum sample to be detected by using whole virus infection, and is assembled into a kit for measuring chicken astrovirus antibodies in serum. The kit can be used for serological diagnosis of chicken astrovirus type II infection, monitoring of the level of antibodies, epidemiological investigation and the like, and provides a novel and effective detection means for prevention and treatment of chicken astrovirus type II.

The invention discloses a preparation method of manganese dioxide nano-enzyme and application of the manganese dioxide nano-enzyme in a disease detection kit.The preparation method of the manganese dioxide nano-enzyme comprises the following steps that a fish peptone solution containing fish peptone powder and a potassium permanganate aqueous solution are mixed to be uniform, a mixed solution is obtained, the mixed solution is stirred to be brown, and a manganese dioxide nano-enzyme solution is obtained; the manganese dioxide nano-enzyme has an ultra-small particle size of 2-5nm, overcomes the problem that the traditional nano-enzyme is small in particle size and easy to agglomerate, and has excellent dispersity and stability; in addition, the manganese dioxide nano-enzyme prepared from the fish peptone powder has high peroxidase-like activity and stability, and the enzyme activity of the manganese dioxide nano-enzyme is 2.36 times higher than that of HRP in a brucellosis detection ELISA kit.

The invention provides a double antibody sandwich ELISA (Enzyme-Linked Immunosorbent Assay) detection kit for a bovine nodular skin disease virus (LSDV), and belongs to the technical field of detection. According to the invention, on the basis of two rabbit monoclonal antibodies (B5-27 and B5-67) aiming at the LSDV B5 protein, the double-antibody sandwich ELISA kit for detecting the LSDV is established. Experiments show that the kit has the advantages of good specificity, high sensitivity, good repeatability and batch detection, and can provide a tool for preventing and controlling bovine nodular dermatosis.