163 results about "Elisa kit" patented technology

The invention relates to the field of biological detection, in particular to a monoclonal antibody combination for detecting human IL-6 protein and application. The combination comprises monoclonal antibodies 3A10 and 2G1, and amino acid sequences of complementary determining regions of variable regions of light and heavy chains of the monoclonal antibodies 3A10 and 2G1 are respectively shown as SEQ ID NO.1-12. The invention defines a complete variable region sequence and a coding nucleotide sequence of the antibody. The combination specifically recognizes human IL-6 recombinant and natural proteins, and has no cross reaction with IL-11. A double-antibody sandwich ELISA kit constructed based on the combination takes 3A10 as a coating antibody and 2G1 as a labeled antibody, and has high sensitivity and high specificity. The problems that an existing reagent is insufficient in specificity and low in sensitivity are solved, a reliable core raw material is provided for IL-6 standardized detection, and the method is suitable for preparation of a kit, a test strip and an antibody chip.

The invention provides an ELISA (Enzyme Linked Immunosorbent Assay) kit for detecting an anti-MDA5 antibody and a preparation method of the ELISA kit. According to the kit, full-length human MDA5 protein (1025 amino acids) expressed by a cell-free protein expression system under the low-temperature condition of 22 DEG C is adopted as a coating antigen, and a disulfide bond enhancer E6820 is added into the system to promote correct folding of the protein. The kit comprises a microwell plate coated with a full-length MDA5 antigen, an enzyme-labeled anti-human IgG secondary antibody, a standard substance with the concentration range of 1-243 ng / mL, a chromogenic substrate and an optimized confining liquid system. The invention solves the problem of insufficient sensitivity caused by the use of fragment antigens in the prior art, and can be used for early diagnosis and illness monitoring of dermatomyositis-related interstitial lung diseases.

The invention belongs to the field of immunological detection, and provides a double-antibody sandwich ELISA kit for detecting the content of human beta-NGF. The enzyme-linked immunosorbent assay kit constructed by the invention is based on a double-antibody sandwich method, and is combined with a biotin-streptavidin signal amplification system and an enzyme developing amplification system, so that the detection sensitivity and specificity are greatly improved, and low-content samples can be accurately and quickly detected. The kit shows excellent stability and specificity and can be used for quantitatively detecting the content of beta-NGF in samples such as human serum, cell supernatant or tissue homogenate, so that an objective detection basis is provided for diagnosis, curative effect evaluation and pathogenesis research of related diseases.

The invention discloses an enzyme linked immunosorbent assay kit for detecting a plasticizer in edible oil and application of the enzyme linked immunosorbent assay kit, and belongs to the technical field of enzyme linked immunosorbent assay. The enzyme linked immunosorbent assay kit comprises an elisa plate coated with a plasticizer coupling antigen, a plasticizer standard substance, an enzyme conjugate working solution, a plasticizer specific antibody working solution, a purification tube, a purification reagent, a standard substance diluent, a sample diluent, a substrate solution, a stop solution and a 10 * concentrated washing solution. The standard substance diluent provided by the invention is used for diluting a plasticizer standard substance at different temperatures to obtain a plasticizer standard solution, and the obtained plasticizer standard solution has basically consistent inhibition ratio, so that the stability of the kit is remarkably enhanced; meanwhile, the standard diluent and other components in the kit have a synergistic effect, so that the enzyme linked immunosorbent assay kit has the advantages of good specificity, high sensitivity, good accuracy and high stability when being used for detecting the plasticizer in the edible oil; therefore, the enzyme linked immunosorbent assay kit has a good application prospect in the detection of the plasticizer in the edible oil.

The application provides an AFP detection kit and application thereof, relates to the technical field of tumor marker detection, and the kit is a double-antibody sandwich ELISA kit. The kit contains two AFP monoclonal antibodies, AFPH8 and AFPE2. The amino acid sequences of the CDR of the heavy chain of AFPH8 are shown in SEQ ID NO. 4-6, the amino acid sequences of the CDR of the light chain of AFPH8 are shown in SEQ ID NO. 7-9, the amino acid sequences of the CDR of the heavy chain of AFPE2 are shown in SEQ ID NO. 12-14, and the amino acid sequences of the CDR of the light chain of AFPE2 are shown in SEQ ID NO. 15-17. Experiments show that the kit has high sensitivity and can detect slightly elevated AFP in initial serum.

This invention discloses a camel-derived CRP single-domain antibody, its preparation method, and its applications, relating to the biomedical field. The method involves enriching CRP antigen in alpaca leukocytes immunized with the antigen, followed by single-cell screening and sequencing. Through sequencing structural analysis, a recombinant plasmid of the camel-derived CRP antibody is constructed. This plasmid is cloned and transformed into eukaryotic cells to express related antibodies. Monoclonal antibodies with human CRP resistance are screened for expression. The VHH domain of the monoclonal antibody is cloned and expressed, and paired screening yields CRP-VHH monoclonal antibodies with antibody activity. This antibody can be used in ELISA kits, chemiluminescence kits, and latex kits, possessing the same functionality as existing kits. Furthermore, this antibody also has applications in the preparation of antitumor drugs.

The utility model provides an ELISA (Enzyme Linked Immunosorbent Assay) kit which comprises a kit body and a kit cover, a partition plate is fixedly connected to the top of the kit body, a drawer is embedded and inserted into the kit body, the top of the partition plate is fixedly connected with a supporting plate through a spring, an insertion plate is fixedly connected to the bottom of the supporting plate, the insertion plate and a limiting plate are correspondingly distributed, and the kit cover is attached to a placing mechanism. And the placing mechanism is fixedly mounted on the top surface of the supporting plate. The kit body is used for placing elisa plates and other reagent bottles, separate storage can be achieved, meanwhile, the reagent bottles are stored and fixed, the stability in the carrying process is ensured, the damage problem is avoided, the elisa plates can be conveniently taken and used by arranging the drawer, the elisa plates can be conveniently stored by utilizing the supporting plate and the placing plate, and the kit is convenient to use. According to the ELISA kit, the drawer can be unlocked by opening the kit cover, and the pressing plate on the kit cover can be used for pressing and storing the ELISA plate, so that the stability of the ELISA plate in the carrying process is ensured, various articles are convenient to take and place, and the use convenience of the ELISA kit is improved.

The invention provides an anti-duck adenovirus 3 type monoclonal antibody, a hybridoma cell strain and application thereof, the anti-duck adenovirus 3 type monoclonal antibody is secreted by a hybridoma cell strain 2F12, the classification name of the hybridoma cell strain 2F12 is Hybridoma cell line 2F12, the hybridoma cell strain 2F12 is preserved in China Center for Type Culture Collection on November 13, 2025, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CGMCC NO. The preservation number of the strain is CCTCC (China Center For Type Culture Collection) NO: The blocking ELISA kit for detecting the duck adenovirus 3 antibody, which is prepared by utilizing the monoclonal antibody, is strong in specificity, high in sensitivity and good in repeatability, and has a very good application prospect in the aspects of epidemiological investigation of the duck adenovirus 3, vaccine immune efficacy evaluation and diagnosis of the duck adenovirus 3 disease.

The invention provides a porcine epidemic diarrhea virus G2c subtype strain and application thereof. According to the invention, the porcine epidemic diarrhea virus G2c subtype HN2501 strain is separated from porcine intestinal tissues and is subjected to passage purification, and the microbial preservation number of the porcine epidemic diarrhea virus G2c subtype HN2501 strain is CGMCC (China General Microbiological Culture Collection Center) No.46417. The isolated strain can stably proliferate on passage cells to generate typical cytopathy. The G2c subtype isolated strain of the porcine epidemic diarrhea virus has excellent immunogenicity; the vaccine prepared from the isolated strain can induce piglets to generate high-level neutralizing antibodies; meanwhile, the ELISA kit prepared from the isolated strain can be used for well detecting the porcine epidemic diarrhea virus antibody in serum.

The invention relates to the technical field of biological detection, and particularly discloses an ELISA kit for detecting a neutralizing antibody of a severe fever with thrombocytopenia syndrome virus and an application method of the ELISA kit. The ELISA kit comprises an ELISA plate on which SFTSV receptor LRP1 protein is fixed, and an enzyme-labeled SFTSV Gn-8M, wherein the SFTSV receptor LRP1 protein is fixed on the ELISA plate; the SFTSV Gn-8M is an SFTSV membrane protein Gn mutant designed by the invention, and can be specifically combined with an SFTSV receptor LRP1 protein on an elisa plate, and compared with a standard virus strain Gn protein, the self-designed SFTSV Gn-8M has amino acid mutation of eight sites, has stronger combining capacity with a host cell receptor, and can be used for detecting the SFTSV. The kit disclosed by the invention has the characteristics of high safety, high efficiency, high flux, high specificity, high sensitivity, high stability and the like, and is suitable for large-scale rapid screening of the neutralizing antibody of the severe fever with thrombocytopenia syndrome virus.

The invention relates to the technical field of biomedicine, and particularly discloses an ARHGAP42 marker for pancreatic cancer diagnosis and prognosis evaluation and application of the ARHGAP42 marker. Experiments prove that the ARHGAP42 is remarkably and highly expressed in pancreatic cancer tissues, and the expression level of the ARHGAP42 is remarkably related to clinical histological grading, lifetime and immunity of a patient. The invention provides application of a reagent for detecting the expression level of ARHGAP42 in preparation of a pancreatic cancer diagnosis or prognosis evaluation tool, including but not limited to qPCR primers, antibodies, ELISA kits or gene chips. In addition, the invention also covers the application of the drug for targeted inhibition of ARHGAP42 in treatment of pancreatic cancer.

The application belongs to the technical field of biology and particularly relates to an ELISA kit for detecting chicken astrovirus type II antibodies and a preparation method. The Cell-ELISA experiment is carried out by using LMH cells infected with chicken astrovirus type II to detect the chicken astrovirus type II antibodies. The method has no cross reactivity with positive serum of Newcastle disease virus, avian influenza virus, chicken infectious anemia virus, avian leukosis virus, Marek's virus and chicken infectious bursal virus, which indicates that the method has good specificity. The Cell-ELISA method is used to detect the chicken serum sample to be detected by using whole virus infection, and is assembled into a kit for measuring chicken astrovirus antibodies in serum. The kit can be used for serological diagnosis of chicken astrovirus type II infection, monitoring of the level of antibodies, epidemiological investigation and the like, and provides a novel and effective detection means for prevention and treatment of chicken astrovirus type II.

The invention provides a double antibody sandwich ELISA (Enzyme-Linked Immunosorbent Assay) detection kit for a bovine nodular skin disease virus (LSDV), and belongs to the technical field of detection. According to the invention, on the basis of two rabbit monoclonal antibodies (B5-27 and B5-67) aiming at the LSDV B5 protein, the double-antibody sandwich ELISA kit for detecting the LSDV is established. Experiments show that the kit has the advantages of good specificity, high sensitivity, good repeatability and batch detection, and can provide a tool for preventing and controlling bovine nodular dermatosis.

The invention provides a method for purifying foot-and-mouth disease based on a method for detecting suspected positive foot-and-mouth disease virus nucleic acid, and aims to thoroughly remove foot-and-mouth disease pathogens in a group and in the environment. According to the provided method, an ELISA kit is used for detection, the kit comprises a monoclonal antibody, the amino acid sequence of a heavy chain of the monoclonal antibody is SEQ ID NO: 7, and the amino acid sequence of a light chain of the monoclonal antibody is SEQ ID NO: 9. The method comprises the following steps: enhancing immunization of pigs with unqualified antibodies, detecting foot-and-mouth disease neutralizing antibodies and VP1 antibodies after immunization, and eliminating immune antibody negative and VP1 antibody positive pigs. The ELISA kit provided by the invention is strong in stability and good in sensitivity, and when the kit is used for checking in a large-scale pig farm, the purification of the foot-and-mouth disease can be effectively realized. The purification method provided by the invention is high in accuracy and easy to popularize and apply.

The invention belongs to the technical field of biological detection, and particularly relates to a monoclonal antibody 5F11 capable of broad-spectrum recognition of various antigens of AAV and an application of the monoclonal antibody 5F11. A heavy chain variable region and a light chain variable region of the monoclonal antibody 5F11 respectively comprise three complementary determining regions which correspond to SEQ ID NO.1-3 and SEQ ID NO.4-6. The monoclonal antibody 5F11 can specifically recognize AAV2, AAV8 and AAV9 antigens, and has the advantages of high sensitivity and broad spectrum. A double-antibody sandwich detection method constructed based on the antibody can detect three AAV antigens at the same time, and the detection cost is remarkably reduced. The clear variable region sequence lays a foundation for humanized transformation, recombinant expression and application in in-vitro detection platforms such as an ELISA kit and an immunochromatography test strip.

The application discloses a CRP camel-derived single-domain antibody and a preparation method and application thereof, relates to the field of biological medicines, and is characterized in that: white blood cells of a llama immunized with a CRP antigen are enriched, and then single-cell screening and sequencing are performed. Through sequencing structure analysis, a camel-derived CRP antibody recombinant plasmid is constructed, a related antibody is expressed in a eukaryotic cell through cloning transformation, a single antibody with human CRP resistance is screened, a VHH domain of the single antibody is cloned and expressed, and a single antibody with CRP-VHH antibody activity is obtained through pairing screening. The antibody can be applied to an ELISA kit, a chemiluminescence kit and a latex kit, has the same functions as existing kits, and still has the use in the preparation of an anti-tumor drug.

The invention provides an ELISA (Enzyme-Linked Immunosorbent Assay) detection kit for detecting rabbit hemorrhagic disease virus infection, and belongs to an in-vitro diagnostic reagent. The invention discloses application of RdRp protein in preparation of a detection kit for detecting rabbit hemorrhagic disease virus infection or distinguishing rabbit hemorrhagic disease virus infection and vaccine immunity. The RdRp protein has good conservative property in RHDV strains of GI.1 and GI.2 types at the same time, and the anti-RdRp protein antibody only exists in a rabbit hemorrhagic disease virus infected body and is not generated in a vaccine immunized body, so that the RdRp protein can be used for distinguishing rabbit hemorrhagic disease virus infection and vaccine immunization based on the specific binding property of the RdRp protein and the anti-RdRp protein antibody; the method greatly simplifies the diagnosis and identification operation, and has the advantages of good accuracy and repeatability, high detection sensitivity, strong detection specificity and great popularization and application value.

The invention belongs to the technical field of Ultrap1 enzyme quantitative detection, and particularly relates to an Ultrap1 enzyme double-antibody sandwich ELISA (enzyme-linked immuno sorbent assay) kit and application. Comprising an elisa plate coated with an anti-Ultrap1 enzyme mouse monoclonal antibody, an anti-Ultrap1 enzyme rabbit polyclonal antibody, an HRP-labeled goat anti-rabbit antibody, a diluent, a cleaning solution, a confining solution, a developing solution and a stop solution. According to the present invention, the complex of the Ulp1 enzyme and the SUMO tag protein is adopted as the immunogen to simultaneously immunize mice and New Zealand rabbits so as to prepare different species of the good affinity Ulp1 enzyme antibodies, and then the optimal reaction condition is further screened to develop the stable double-antibody sandwich ELISA detection kit with characteristics of high specificity and strong sensitivity, the defects in the prior art can be effectively overcome.

This invention relates to the field of biotechnology, and aims to provide a monoclonal antibody 7A12 for identifying genotype 2 classical swine fever, along with its preparation and application methods. This monoclonal antibody contains an Ig domain V of the antibody heavy chain. H CDR1, V H CDR2 and V H CDR3, and the Ig domain V of the antibody light chain L CDR1, V L CDR2 and V L CDR3; wherein, the V H CDR1, V H CDR2 and V H The amino acid sequences of CDR3 are shown in SEQ ID NO:1-3, respectively; the V L CDR1, V L CDR2 and V L The amino acid sequences of CDR3 are shown in SEQ ID NO:4-6. The antibody provided by this invention has advantages such as high titer, strong specificity, and high affinity; it also has high sensitivity and specificity, and shows no cross-reactivity with type 1 classical swine fever vaccine strains. Serologically, it can differentiate and diagnose infection and vaccine immunization with genotype 2 classical swine fever wild-type strains. Furthermore, it can be used to develop a blocking ELISA kit for identifying genotype 2 classical swine fever, effectively diagnosing and purifying genotype 2 classical swine fever virus.

The invention discloses a biomarker for diagnosing obesity and complications thereof and application of the biomarker. The biomarker is one or a combination of more of ADAM8, FASN and ELOVL6. The invention also provides a kit for diagnosing obesity and complications thereof. The kit comprises a reagent for detecting the expression level of one or more proteins of ADAM8, FASN and ELOVL6. According to the present invention, the expression levels of ADAM8, FASN and ELOVL6 in serum are detected by using the ELISA kit, such that the sensitivity and the specificity are high. The method is easy and convenient to operate and accurate in result, early diagnosis and intervention of obesity-related hyperlipemia are facilitated, and the burden of patients and the society is relieved.

The application discloses an antigen peptide targeting DRP1 protein Ser572 phosphorylation, an antibody and application thereof, and particularly relates to the technical field of biological medicines, and the antigen peptide has immunogenicity of targeting a DRP1 protein Ser572 phosphorylation site, wherein the amino acid sequence of the antigen peptide is RETKNVA{piSer}GGGGVGC, and {piSer} indicates that serine at the site is modified by phosphorylation. The antibody prepared by the application can stably recognize DRP1 Ser572 phosphorylation modification at a cell and animal level, can clearly reveal a molecular mechanism of the site phosphorylation in regulating DRP1 liquid-liquid phase separation and mediating myocardial mitochondrial damage, can support basic mechanism research of diseases such as sepsis, can be used for developing a specific ELISA kit and a pharmaceutical composition, fills a technical gap of DRP1 Ser572 phosphorylation related research and clinical transformation, and provides a brand-new technical support for diagnosis and treatment of cardiovascular diseases, sepsis and other major diseases.

The application provides an anti-human TL1A monoclonal antibody and a related kit and detection method thereof. The ELISA kit of the application can quantitatively detect the content of TL1A in various samples such as cell culture medium, is simple and convenient to operate, has high sensitivity, has good specificity, and can detect TL1A of different species, and has important significance for the research on the pathogenesis of TL1A related autoimmune diseases and drug models.

The application belongs to the field of immunological detection, and provides a double-antibody sandwich ELISA kit for detecting human Cyfra21-1 content. The anti-human Cyfra21-1 monoclonal antibody provided by the application can specifically bind to human Cyfra21-1, has no cross reaction with other similar proteins, and has high stability; based on the antibody, the double-antibody sandwich ELISA method for detecting human Cyfra21-1 has high sensitivity, specificity and accuracy, and can accurately and high-throughput detect the content of Cyfra21-1 in human serum or other serum analogs containing human Cyfra21-1.

The application provides a porcine epidemic diarrhea virus mutant strain and application thereof. The application obtains a porcine epidemic diarrhea virus mutant strain GX2506 from porcine intestinal tissue, and the strain is purified through passage, and the microorganism preservation number of the strain is CGMCC No. 47089. The isolated strain can stably proliferate on a passage cell, and typical cytopathic effect is generated. The porcine epidemic diarrhea virus mutant isolated strain has excellent immunogenicity; a vaccine prepared by using the isolated strain can induce piglets to generate high-level neutralizing antibodies; meanwhile, an ELISA kit prepared by using the isolated strain can better detect porcine epidemic diarrhea virus antibodies in serum.

The application discloses two new anti-anti-thymic stromal lymphopoietin (TSLP) monoclonal antibodies, and an ELISA kit comprising the antibodies and a detection method thereof. The antibodies disclosed by the application comprise antibody heavy chain complementarity determining regions and antibody light chain complementarity determining regions, and the ELISA kit comprises two antibodies. The capture antibody, specifically, the anti-anti-thymic stromal lymphopoietin (TSLP) monoclonal antibody, is used as coating; the second antibody, specifically, the biotin-labeled anti-anti-thymic stromal lymphopoietin (TSLP) monoclonal antibody, is used as binding with the capture antibody-antibody complex. The kit can be used for successfully quantitatively detecting the anti-thymic stromal lymphopoietin (TSLP) monoclonal antibody, has high detection sensitivity and good detection specificity, and has a good development and application prospect.

This invention discloses anti-leptin monoclonal antibodies and their applications, relating to the field of biotechnology. This invention develops anti-leptin monoclonal antibodies 2A122H7 and 6B62E3, which can effectively recognize leptin. The ELISA kits prepared using these monoclonal antibodies 2A122H7 and 6B62E3 include indirect ELISA and double-antibody sandwich ELISA kits, exhibiting high specificity and sensitivity for the tested samples, and are convenient, efficient, and cost-effective. This invention provides effective technical support for advancing research on the regulatory mechanisms of economic traits such as feed intake, fat deposition, and reproduction in poultry.

The application provides a PLA2R monoclonal antibody and an ELISA kit comprising the antibody. Specifically, the application provides a monoclonal antibody which specifically binds to PLA2R, wherein the antibody only binds to non-reduced PLA2R antigen with a conformational epitope, does not bind to reduced PLA2R antigen, and does not bind to another antigen THSD7A of primary membranous nephropathy. An ELISA kit prepared based on the antibody can more efficiently and accurately detect primary membranous nephropathy than existing commercial kits.

The present application relates to the technical field of biology, in particular to a streptococcus pneumoniae urine antigen ELISA kit and a preparation method thereof.The solid-phase coating material of the kit is CRP, which is easy to prepare and obtain and low in cost;the reaction system only needs one antibody, greatly reducing the workload and labor cost of screening and preparation of paired antibodies;the antigen detection is fully automatic, greatly increasing the detection throughput and reducing the labor cost.

The present disclosure relates to the field of biotechnology, and particularly relates to detection of BXB1 integrase. The present disclosure establishes a method for detecting BXB1 integrase based on double-antibody sandwich ELISA technology, which has good sensitivity and specificity, and the method is simple to operate and can quickly realize accurate quantification of BXB1 integrase; in addition, the present disclosure also provides an ELISA kit for detecting BXB1 integrase. By using the BXB1 integrase detection method or kit provided by the present disclosure, accurate quantification of the residual amount of BXB1 integrase in various intermediate products, semi-finished products or finished products in the process of preparing recombinant biological products by the BXB1 integrase system can be realized, which is beneficial to the quality control of products by biopharmaceutical enterprises.

This invention provides a serum protein biomarker for early screening and diagnosis of pancreatic ductal adenocarcinoma. The serum protein biomarker is one or a combination of two or more autoantibodies against tumor-associated antigens HEXB, TXLNA, and SLAMF6. The invention also provides the application of reagents, kits, and analytical methods for detecting the serum protein biomarker. This invention screens for autoantibodies against pancreatic ductal adenocarcinoma-associated antigens. By measuring the expression levels of these indicators in the serum of patients with pancreatic ductal adenocarcinoma, healthy controls, and controls with benign pancreatic diseases, the screened anti-HEXB, anti-TXLNA, and anti-SLAMF6 autoantibodies can distinguish between pancreatic ductal adenocarcinoma, healthy controls, and benign pancreatic diseases. Furthermore, this invention designs and optimizes combinations of tumor-associated antigens for the diagnosis of pancreatic ductal adenocarcinoma. Applying the optimized antigens to an ELISA kit and detecting serum anti-TAA autoantibodies using an indirect enzyme-linked immunosorbent assay (ELISA) can accurately identify patients with pancreatic ductal adenocarcinoma, healthy controls, and patients with benign pancreatic diseases.