255results about "Ovalbumin" patented technology

This invention discloses a milk protein containing composition comprising: at least one milk protein; at least a first protein; at least a second protein; and at least a linker comprising an amino acid sequence coded by nucleotide sequence which is at least 80% similar to sequences selected from the group consisting of SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, and SEQ ID NO: 21

The invention belongs to the technical field of biochemical engineering, and particularly relates to an amitriptyline hapten and a preparation method thereof, an amitriptyline antigen and a preparation method thereof, an antibody, a kit and application. The amitriptyline hapten has a structural formula shown as a formula (I) or a formula (II). Compared with an existing structure, the amitriptyline hapten prepared by the invention has a better immune effect. The obtained antibody is high in titer, strong in specificity and high in affinity, the LOD of amitriptyline by using an Elisa method established by the antibody is 0.013 ng / mL, the IC50 of amitriptyline is 0.865 ng / mL, the quantitative detection range is 0.07-470.3 ng / mL, the detection sensitivity is high, and the linear range is wide.

The invention discloses a trimethoprim hapten as well as synthesis and application thereof. The structure of the trimethoprim hapten is as shown in a formula I. The trimethoprim hapten provided by the invention can fully expose the antigenic determinant of trimethoprim and maintain the spatial conformation of trimethoprim, has good immunogenicity, and is mild in synthesis conditions, few in reaction steps, high in yield and capable of reducing the cost of raw materials. The trimethoprim colloidal gold detection product provided by the invention has the characteristics of strong specificity and high sensitivity, can realize accurate and rapid detection of trimethoprim in a sample, and has no cross reaction with other drugs. And the formula I of the # imgabs0 # is shown in the specification.

The invention provides a safrole hapten and application of the safrole hapten in detection of safrole through immunoassay. The structure of the safrole hapten is shown in the specification. The two safrole haptens provided by the invention have spacer arms with proper lengths, and after the safrole haptens are coupled with carrier protein, the haptens can be fully exposed, and recognition of the spacer arms can be avoided as much as possible. The safrole hapten is used for preparing an artificial antigen and an antibody, and the obtained safrole monoclonal antibody is high in titer, strong in specificity and high in affinity. An immunoassay method constructed on the basis of the safrole artificial antigen and antibody has high specificity and sensitivity, the lowest detection limit LOD is 0.09 ng / mL, the half inhibitory concentration IC50 is 2.05 ng / mL, the quantitative detection range is 0.28-14.89 ng / mL, no cross reaction exists on safrole analogues, and rapid qualitative and quantitative detection can be conducted on safrole in a sample.

The invention discloses a hapten and an artificial antigen for detecting chlorfenapyr and application of the hapten and the artificial antigen. A time-resolved fluorescent test strip for detecting chlorfenapyr is prepared from the artificial antigen. The kit has the characteristics of high specificity, high sensitivity, high precision, high accuracy and the like, the quantitative detection limit of chlorfenapyr is as low as 3.48 ng / mL, the addition recovery rate is 99.6%-110.4%, the coefficient of variation is 2.1%-10.9%, and the kit is very suitable for rapid qualitative or quantitative detection of chlorfenapyr in plant-derived food by basic field personnel.

The invention discloses a phthalimidic acid hapten, an artificial antigen, an antibody, and a preparation method and application of the phthalimidic acid hapten and the artificial antigen. The structure of the phthalimide acid hapten is shown as a formula (I), and the phthalimide acid hapten is obtained by introducing a carboxyl active group with a spacer arm with a certain length to carboxyl of phthalimide acid, so that the phthalimide acid hapten can be coupled with carrier protein to obtain an artificial antigen for immunization; the phthalimide hapten retains all characteristic groups of the phthalimide, the original structural characteristics of the phthalimide are changed to the minimum extent, and a foundation is laid for generating an antibody with higher specificity and higher sensitivity for subsequently stimulating animal immune response. An artificial antigen and a phthalimide acid monoclonal antibody are prepared through the hapten, indirect competitive ELISA based on the monoclonal antibody has the lowest detection limit of phthalimide acid of 2.44 ng / mL, IC50 of 17.29 ng / mL and the linear range of 5.02-59.51 ng / mL, the detection sensitivity is high, and the linear range is wide.

The invention relates to the technical field of antibodies, in particular to antigen peptides of human PP1 alpha protein and Thr320 site phosphorylated protein of the human PP1 alpha protein, and a preparation method and application of an antibody of the antigen peptides of the human PP1 alpha protein and the Thr320 site phosphorylated protein. The invention provides an antigen peptide of human PP1 alpha protein and Thr320 site phosphorylated protein thereof, the antigen peptide has obvious advantages in the aspects of immunogenicity and the like, animals can be induced to generate high-level antibodies, and the generated antibodies have high specificity, affinity and titer. Based on the antigen peptide, the invention provides an anti-human PP1 alpha protein or a polyclonal antibody with phosphorylated Thr320 site thereof and a preparation method thereof, the polyclonal antibody has the characteristics of strong specificity, high affinity and high titer, and through immunoblotting and immunohistochemical verification, the polyclonal antibody can be used for preparing the anti-human PP1 alpha protein or the anti-human PP1 alpha protein or the anti-human PP1 alpha protein or the anti-human PP1 alpha protein or the anti-human PP1 alpha protein. The probe shows good specificity and sensitivity in detection of phosphorylation modification of human PP1 alpha protein and Thr320 site thereof, and has a good application prospect.

The invention provides an analgin hapten, a preparation method thereof, an artificial antigen, an antibody and application. The structures of the analgin haptens are as shown in a formula (I) or a formula (II), the two analgin haptens provided by the invention have spacer arms with proper lengths, and after the two analgin haptens are coupled with protein, the haptens can be fully exposed to an immune system, the spacer arms can be prevented from being recognized as much as possible, and a better antibody is generated to recognize analgin. The analgin hapten is used for preparing artificial antigen and antibody, the obtained analgin monoclonal antibody has high titer and strong specificity, an immunoassay method constructed based on the analgin monoclonal antibody has high specificity and sensitivity, analgin in a sample can be rapidly, qualitatively and quantitatively detected, the operation is simple and convenient, and the detection result is accurate and reliable.

The invention relates to the technical field of bioengineering, in particular to a capsid armed adenovirus carrying an antigen in a pIX region, a construction method and application. Introducing an exogenous antigen gene into a secondary capsid protein pIX region of the type 5 adenovirus to form a capsid armed adenovirus; the type-5 adenovirus specifically has E1 region and E3 region deletion. According to the construction method, pIX is used as a display platform, an exogenous antigen can be presented on the surface of a virus in a high-density and high-repeatability manner, the effective concentration of the antigen is greatly improved, and stronger and more lasting specific immune response is expected to be induced; the modified pIX protein has smaller influence on packaging, assembling and structural stability of viruses and infection ability on target cells; the adenovirus can realize dual functions of gene delivery and surface antigen presentation, and provides an innovative technical platform for developing multifunctional and high-titer vaccines or gene therapy drugs.

The application discloses a kind of diflufenican hapten, antigen, antibody and its preparation method and application.The application prepares diflufenican hapten as shown in formula (I) and corresponding diflufenican antigen, and specific antibody for detecting diflufenican is prepared by diflufenican antigen, and further constructs the immune analysis method for directly detecting diflufenican pesticide residue, which is particularly suitable for on-site rapid detection, and can meet the needs of regulatory authorities and detection agencies formula (I).

The present invention discloses a diflubenzuron hapten, antigen, antibody, detection device, and their preparation and application. It also relates to a diflubenzuron hapten, a diflubenzuron antigen, a diflubenzuron antibody, and a colloidal gold chromatography detection device for diflubenzuron, as well as their preparation and application for detecting diflubenzuron. The present invention utilizes an immunological detection method to specifically detect residual diflubenzuron in a sample. The method has the advantages of good specificity, high sensitivity, ease of operation, and the ability to achieve rapid on-site detection. It can better meet the needs of regulatory authorities and testing agencies for on-site supervision and law enforcement, and provides technical support for relevant law enforcement agencies in rapidly detecting residual diflubenzuron in vegetables and fruits.

The present invention relates to the technical field of haptens, and particularly to a bongkrekic acid hapten, a preparation method thereof and an application. On the basis of the structure of bongkrekic acid, the present invention selects two intermediates with partial structures similar to the two ends thereof respectively for modification and coupling, adds an alanine coupling arm at a specific site, and prepares a bongkrekic acid hapten with an active amino group. After biological coupling with a carrier protein, the corresponding artificial complete antigen of bongkrekic acid can be obtained; by using the artificial complete antigen of bongkrekic acid, an antibody against bongkrekic acid can be prepared, which can be used to quickly and accurately detect bongkrekic acid in food.

The application relates to a detection method of oxicams for non-diagnostic purposes, and the application obtains a new oxicam hapten by optimizing the design of spatial configuration and chemical characteristics, and significantly improves the immunization efficiency of an antibody and the performance of an immunodetection device. The universal oxicam immunodetection device developed by the application integrates high sensitivity, wide detection range, anti-interference capability and rapid portability, overcomes the defects of the prior art, provides an efficient and reliable solution for rapid screening of oxicams in samples, and has significant technical progress and wide application prospect.

This invention relates to the field of bioengineering technology, specifically to a recombinant adenovirus displaying a large antigen fragment on its capsid surface, its construction method, and its applications. The HVR1 and HVR5 regions of the hexon hypervariable region of this virus are replaced by gene fragments containing exogenous genes; each exogenous gene fragment has a flexible linker attached to both ends, and the gene sequence of the flexible linker is shown in SEQ ID No. 1. By precisely locating the naturally variable HVR1 and HVR5 loop regions exposed on the surface of the hexon protein as insertion sites, and supplementing them with flexible linkers, the core problem of exogenous sequence insertion disrupting the stability of the hexon structure is successfully solved. This allows for stable packaging to produce viral particles with high titers and infectivity, enabling the development of a new generation of highly efficient and safe capsid-displaying adenovirus vaccines.

The invention discloses design and synthesis of a hapten of cyromazine, development of a cyromazine artificial antigen and a cyromazine antibody, and application of the cyromazine artificial antigen and the cyromazine antibody in the field of food safety rapid detection. The aminocarboxylic acid modified cyromazine hapten product is prepared through the steps of cyromazine hapten design, connecting arm introduction, synthesis, purification and the like. The cyromazine hapten with the structure is coupled with keyhole limpet hemocyanin to form an artificial immune antigen with immunogenicity, and is coupled with chicken ovalbumin to form an artificial competitive antigen. Tests show that the artificial immune antigen has high immunocompetence and can stimulate an animal immune system to generate an anti-cyromazine antibody, and the titer of an immune Blab / c mouse serum antibody is 16000. The IC50 of an enzyme-linked immunosorbent assay (ELISA) detection method established by the cyromazine artificial competitive antigen and the anti-cyromazine antibody is 5.3 [mu] g / L, and the detection sensitivity of an established gold-labeled immunochromatography detection method reaches 50 [mu] g / L.

The invention provides a homocysteine hapten as well as a preparation method and application thereof. The hapten is prepared from L-homocysteine through a coupling reaction of sulfydryl and amino of 2, 4, 5-triamino-6-chloropyrimidine, and the hapten is obtained through a coupling reaction of sulfydryl and amino of 2, 4, 5-triamino-6-chloropyrimidine. The hapten is coupled with a complete antigen formed by carrier protein. The antigen can form strong immunoreaction and is beneficial to screening of specific Hcy antibodies.

The present invention discloses amitraz hapten, antigen, antibody, detection device and preparation and application thereof, and relates to amitraz hapten, antigen, antibody, colloidal gold detection device and preparation and application thereof for detecting amitraz. The present invention provides a method for preparing artificial antigen by synthesizing amitraz hapten and coupling antigen with carrier protein, as well as antibodies specific to amitraz produced by the body of experimental animals after immunization, and the IC 50 The value was 5.22 ng / mL, and the linear detection range for amitraz was 1.82-14.96 ng / mL, which meets the limit for amitraz in the national standard GB 2763-2021, "National Food Safety Standard Maximum Limits of Pesticide Residues in Food." Therefore, the present invention can be applied to the rapid detection of amitraz residues in agricultural products.

The invention discloses a halogen modified hapten for enhancing antibody titer and affinity as well as a preparation method and application of the halogen modified hapten, and belongs to the technical field of biochemical engineering. According to the method, an amantadine structure is innovatively and chemically modified, various halogens such as fluorine, chlorine and bromine are introduced, the AMA halogen modified hapten shown in the formula (I) is formed, and the traditional'most similar 'design principle is broken through. Compared with an unmodified hapten, the hapten modified by halogen in the invention has the advantages that the titer of the polyclonal antibody on amantadine can be obviously improved by 3.4-14 times; wherein the haptens A5 and A6 can obviously improve the titer of the monoclonal antibody to amantadine by 3-18 times, and the affinity is improved by 10.8-16.1 times. The invention provides a new thought and a new method for the rational design of the hapten of the small molecule compound and the preparation of the high-affinity antibody, and the related halogen modification type and quantity strategy can also provide valuable reference for the design of the hapten of other small molecule compounds.

The invention discloses a novobiocin hapten as well as synthesis and application thereof. The structure of the novobiocin hapten is as shown in formula I in the specification. The neomycin hapten provided by the invention can fully expose the antigenic determinant of the neomycin and maintain the spatial conformation of the neomycin, has good immunogenicity, and is mild in synthesis conditions, few in reaction steps, high in yield and capable of reducing the cost of raw materials. The novobiocin colloidal gold detection product provided by the invention has the characteristics of strong specificity and high sensitivity, can realize accurate and rapid detection of novobiocin in a sample, and has no cross reaction with other drugs. And the formula I of the # imgabs0 # is shown in the specification.

Provided herein are compositions with enhanced protein content, compositions with functional proteins, protein combinations and methods for the preparation thereof.

The present invention relates to the field of immunoassay technology and provides a bifenthrin hapten, antigen, antibody, preparation method, and application thereof. The bifenthrin hapten provided by the present invention completely retains the molecular structure of bifenthrin. After coupling with a carrier protein, the immunogenicity of the resulting antigen is significantly improved, and the prepared antibody has stronger specificity and higher sensitivity. The results of the examples show that the bifenthrin hapten provided by the present invention is coupled with a carrier protein, and the resulting antigen, after injection into mice for immunization, produces an antibody titer of 1×10 4 , half inhibitory concentration (IC 50 ) is about 20ppb, and the minimum detection limit of colloidal gold for bifenthrin in dry tobacco leaf samples is about 1.25μg / g.

Food products are provided in which microbial protein sources, such as single cell protein or protein hydrolysis products, are incorporated, and which contain proteins that are recombinantly expressed in chemoautotrophic microorganisms. Meat, dairy, and egg substitute products are provided that include microbial protein and proteins that are recombinantly produced in chemoautotrophic microorganisms in place of animal protein.

The invention belongs to the technical field of polycyclic aromatic hydrocarbon artificial antigen synthesis, and relates to a novel synthetic method of a complete antigen for detecting polycyclic aromatic hydrocarbon (PAHs)-# imgabs0 #. The hapten is successfully prepared through Friedel-Crafts acylation reaction, and the hapten is esterified and then coupled with carrier protein to prepare the complete antigen. These complete antigens have been identified. The invention provides a novel hapten screening and preparation method and an efficient artificial antigen preparation method for an immunoassay method of # imgabs1 #.

The present application relates to the technical field of bioengineering, and particularly relates to a pIX region antigen-carrying capsid armed adenovirus, a construction method and application. An exogenous antigen gene is introduced into a pIX region of a minor capsid protein of a type 5 adenovirus to form a capsid armed adenovirus; the type 5 adenovirus specifically has an E1 region and an E3 region deletion. The construction method uses pIX as a display platform, can present an exogenous antigen on the virus surface with high density and high repeatability, greatly improves the effective concentration of the antigen, and is expected to induce stronger and more persistent specific immune responses; the modified pIX protein has less influence on the packaging, assembly, structural stability of the virus and the infection ability on target cells; the adenovirus can realize the dual functions of "gene delivery" and "surface antigen presentation", and provides an innovative technical platform for developing multifunctional and high-titer vaccines or gene therapy drugs.

The invention discloses a method for detecting malachite green, crystal violet and methylene blue, which is characterized in that two different haptens are respectively used as an immunogen and a coating antigen to obtain a high-sensitivity monoclonal antibody for resisting malachite green, crystal violet and methylene blue. The invention further establishes a chemiluminescence immune method for detecting malachite green, crystal violet and methylene blue based on biotin-streptavidin, and the method has the advantages of rapidness, sensitivity, low cost and high throughput, and is suitable for simultaneously detecting the residual quantity of malachite green, crystal violet or methylene blue in aquatic products.

The present application relates to the technical field of hapten, in particular to vitamin D3 carboxylic acid derivative, antigen and preparation method thereof. The structural formula of vitamin D3 carboxylic acid derivative is shown as follows: the vitamin D3 carboxylic acid derivative has excellent antibody binding activity, the vitamin D3 antigen coupled has high activity and inhibition rate. And the synthesis cost of the vitamin D3 carboxylic acid derivative is low, the cycle is short, and the yield is high.

The present invention discloses a diafenthiuron hapten, an artificial antigen, preparation methods, antibodies, and applications thereof. The structural formula of the diafenthiuron hapten is #imgabs0#. The hapten, artificial antigen, antibodies to immunized animal products, and the test kit prepared in this invention exhibit high sensitivity and specificity, are simple to operate, have a short detection time, are not limited by specialized personnel, are inexpensive, and are suitable for grassroots deployment. This technology fills a gap in domestic rapid detection of diafenthiuron. The application of this technology can significantly accelerate detection and has significant social benefits in controlling diafenthiuron pesticide poisoning in edible agricultural products, reducing pesticide over-limits, improving the qualified rate of commercially available vegetables, and ensuring food safety.

The invention discloses amitraz hapten, antigen, antibody and detection device as well as preparation and application thereof, and relates to amitraz hapten, antigen, antibody and colloidal gold detection device as well as preparation and application thereof in amitraz detection. The invention provides a method for preparing an artificial antigen from amitraz hapten synthesis and antigen coupling carrier protein, and a specific amitraz antibody generated by the body of an immune experimental animal after the immune experimental animal, the IC50 value of the antibody is 5.22 ng / mL, the linear detection range of amitraz is 1.82-14.96 ng / mL, and the detection result shows that the antibody can be used for detecting amitraz. And the amitraz composition accords with the limit regulation of amitraz in the national standard GB 2763-2021 National Food Safety Standard Maximum Pesticide Residue Limit in Food. Therefore, the method can be applied to rapid detection of the residual content of amitraz in agricultural products.

Provided herein are methods for preparing a protein composition having a reduced quantity of a recombinant cell byproduct which provides an undesirable color to a protein. The methods involve the use of absorbents or flocculants that capture, trap or isolate the recombinant cell byproduct. The methods also involve oxidation of the recombinant cell byproduct with an oxidizing agent (e.g. hydrogen peroxide) or light (e.g. photochemical oxidation).

The invention discloses a cyenopyrafen hapten, a cyenopyrafen antigen, a cyenopyrafen antibody, a preparation method and an application thereof. The preparation method comprises the following steps: reacting a hydrolysate B with an ester group-containing compound to obtain a carboxyl-containing hapten compound with a structural formula shown as A1; carrying out first coupling reaction on the hapten compound A1 and N-hydroxysuccinimide to obtain a precursor compound; the precursor compound and a carrier protein are subjected to a second coupling reaction to obtain an antigen with a structural formula shown as A2, and # imgabs1 # can be further prepared into a cyenopyrafen antibody with good specificity and high sensitivity through the preparation method, so that rapid, accurate and low-cost detection of cyenopyrafen is realized.