113results about "Ovalbumin" patented technology

This invention discloses a milk protein containing composition comprising: at least one milk protein; at least a first protein; at least a second protein; and at least a linker comprising an amino acid sequence coded by nucleotide sequence which is at least 80% similar to sequences selected from the group consisting of SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, and SEQ ID NO: 21

The invention belongs to the technical field of biochemical engineering, and particularly relates to an amitriptyline hapten and a preparation method thereof, an amitriptyline antigen and a preparation method thereof, an antibody, a kit and application. The amitriptyline hapten has a structural formula shown as a formula (I) or a formula (II). Compared with an existing structure, the amitriptyline hapten prepared by the invention has a better immune effect. The obtained antibody is high in titer, strong in specificity and high in affinity, the LOD of amitriptyline by using an Elisa method established by the antibody is 0.013 ng / mL, the IC50 of amitriptyline is 0.865 ng / mL, the quantitative detection range is 0.07-470.3 ng / mL, the detection sensitivity is high, and the linear range is wide.

The invention discloses a phthalimidic acid hapten, an artificial antigen, an antibody, and a preparation method and application of the phthalimidic acid hapten and the artificial antigen. The structure of the phthalimide acid hapten is shown as a formula (I), and the phthalimide acid hapten is obtained by introducing a carboxyl active group with a spacer arm with a certain length to carboxyl of phthalimide acid, so that the phthalimide acid hapten can be coupled with carrier protein to obtain an artificial antigen for immunization; the phthalimide hapten retains all characteristic groups of the phthalimide, the original structural characteristics of the phthalimide are changed to the minimum extent, and a foundation is laid for generating an antibody with higher specificity and higher sensitivity for subsequently stimulating animal immune response. An artificial antigen and a phthalimide acid monoclonal antibody are prepared through the hapten, indirect competitive ELISA based on the monoclonal antibody has the lowest detection limit of phthalimide acid of 2.44 ng / mL, IC50 of 17.29 ng / mL and the linear range of 5.02-59.51 ng / mL, the detection sensitivity is high, and the linear range is wide.

The application discloses a kind of diflufenican hapten, antigen, antibody and its preparation method and application.The application prepares diflufenican hapten as shown in formula (I) and corresponding diflufenican antigen, and specific antibody for detecting diflufenican is prepared by diflufenican antigen, and further constructs the immune analysis method for directly detecting diflufenican pesticide residue, which is particularly suitable for on-site rapid detection, and can meet the needs of regulatory authorities and detection agencies formula (I).

The application relates to a detection method of oxicams for non-diagnostic purposes, and the application obtains a new oxicam hapten by optimizing the design of spatial configuration and chemical characteristics, and significantly improves the immunization efficiency of an antibody and the performance of an immunodetection device. The universal oxicam immunodetection device developed by the application integrates high sensitivity, wide detection range, anti-interference capability and rapid portability, overcomes the defects of the prior art, provides an efficient and reliable solution for rapid screening of oxicams in samples, and has significant technical progress and wide application prospect.

This invention relates to the field of bioengineering technology, specifically to a recombinant adenovirus displaying a large antigen fragment on its capsid surface, its construction method, and its applications. The HVR1 and HVR5 regions of the hexon hypervariable region of this virus are replaced by gene fragments containing exogenous genes; each exogenous gene fragment has a flexible linker attached to both ends, and the gene sequence of the flexible linker is shown in SEQ ID No. 1. By precisely locating the naturally variable HVR1 and HVR5 loop regions exposed on the surface of the hexon protein as insertion sites, and supplementing them with flexible linkers, the core problem of exogenous sequence insertion disrupting the stability of the hexon structure is successfully solved. This allows for stable packaging to produce viral particles with high titers and infectivity, enabling the development of a new generation of highly efficient and safe capsid-displaying adenovirus vaccines.

The invention discloses design and synthesis of a hapten of cyromazine, development of a cyromazine artificial antigen and a cyromazine antibody, and application of the cyromazine artificial antigen and the cyromazine antibody in the field of food safety rapid detection. The aminocarboxylic acid modified cyromazine hapten product is prepared through the steps of cyromazine hapten design, connecting arm introduction, synthesis, purification and the like. The cyromazine hapten with the structure is coupled with keyhole limpet hemocyanin to form an artificial immune antigen with immunogenicity, and is coupled with chicken ovalbumin to form an artificial competitive antigen. Tests show that the artificial immune antigen has high immunocompetence and can stimulate an animal immune system to generate an anti-cyromazine antibody, and the titer of an immune Blab / c mouse serum antibody is 16000. The IC50 of an enzyme-linked immunosorbent assay (ELISA) detection method established by the cyromazine artificial competitive antigen and the anti-cyromazine antibody is 5.3 [mu] g / L, and the detection sensitivity of an established gold-labeled immunochromatography detection method reaches 50 [mu] g / L.

The invention discloses a halogen modified hapten for enhancing antibody titer and affinity as well as a preparation method and application of the halogen modified hapten, and belongs to the technical field of biochemical engineering. According to the method, an amantadine structure is innovatively and chemically modified, various halogens such as fluorine, chlorine and bromine are introduced, the AMA halogen modified hapten shown in the formula (I) is formed, and the traditional'most similar 'design principle is broken through. Compared with an unmodified hapten, the hapten modified by halogen in the invention has the advantages that the titer of the polyclonal antibody on amantadine can be obviously improved by 3.4-14 times; wherein the haptens A5 and A6 can obviously improve the titer of the monoclonal antibody to amantadine by 3-18 times, and the affinity is improved by 10.8-16.1 times. The invention provides a new thought and a new method for the rational design of the hapten of the small molecule compound and the preparation of the high-affinity antibody, and the related halogen modification type and quantity strategy can also provide valuable reference for the design of the hapten of other small molecule compounds.

Food products are provided in which microbial protein sources, such as single cell protein or protein hydrolysis products, are incorporated, and which contain proteins that are recombinantly expressed in chemoautotrophic microorganisms. Meat, dairy, and egg substitute products are provided that include microbial protein and proteins that are recombinantly produced in chemoautotrophic microorganisms in place of animal protein.

The present application relates to the technical field of bioengineering, and particularly relates to a pIX region antigen-carrying capsid armed adenovirus, a construction method and application. An exogenous antigen gene is introduced into a pIX region of a minor capsid protein of a type 5 adenovirus to form a capsid armed adenovirus; the type 5 adenovirus specifically has an E1 region and an E3 region deletion. The construction method uses pIX as a display platform, can present an exogenous antigen on the virus surface with high density and high repeatability, greatly improves the effective concentration of the antigen, and is expected to induce stronger and more persistent specific immune responses; the modified pIX protein has less influence on the packaging, assembly, structural stability of the virus and the infection ability on target cells; the adenovirus can realize the dual functions of "gene delivery" and "surface antigen presentation", and provides an innovative technical platform for developing multifunctional and high-titer vaccines or gene therapy drugs.

The present application relates to the technical field of hapten, in particular to vitamin D3 carboxylic acid derivative, antigen and preparation method thereof. The structural formula of vitamin D3 carboxylic acid derivative is shown as follows: the vitamin D3 carboxylic acid derivative has excellent antibody binding activity, the vitamin D3 antigen coupled has high activity and inhibition rate. And the synthesis cost of the vitamin D3 carboxylic acid derivative is low, the cycle is short, and the yield is high.

Provided herein are methods for preparing a protein composition having a reduced quantity of a recombinant cell byproduct which provides an undesirable color to a protein. The methods involve the use of absorbents or flocculants that capture, trap or isolate the recombinant cell byproduct. The methods also involve oxidation of the recombinant cell byproduct with an oxidizing agent (e.g. hydrogen peroxide) or light (e.g. photochemical oxidation).

The present invention provides a conjugate compound comprising a nanoparticulated enhanced permeability and retention (EPR) agent, which is an albumin, conjugated with one or more chelating agents, one or more target specific ligands and one or more detectable agents and optionally comprising one or more heterobifunctional crosslinkers and one or more pharmaceutically acceptable therapeutic agents. Further, the present invention provides a radiopharmaceutical composition and a kit comprising the said conjugate and also provides application of the same in diagnosis and therapy of various disease like cancer.

The invention relates to a biocompatible skin wound healing medicine and a preparation method thereof, and belongs to the technical field of biological medicine, and the medicine comprises liposome-clathrated ovalbumin peptide, a red abelmoschus esculentus seed extract, a taro stem extract, liposome-clathrated acetyl tetrapeptide-9 and vitamin E. The ovalbumin peptide is prepared by the following steps: pre-cooling ovalbumin of goose eggs, sequentially carrying out enzymolysis on the pre-cooled ovalbumin through trypsin and ginger protease, and purifying the enzymatic hydrolysate through an XAD16 nonionic macroporous resin column. The hibiscus coccineus seed extract is obtained by performing ultrasonic enzymolysis on hibiscus coccineus seeds in an ethanol water solution through hemicellulase and ginger protease and purifying enzymatic hydrolysate through an AB-8 macroporous adsorption resin column. The taro stalk extract is obtained by grinding taro stalks into pulp, performing ultrasonic extraction with an ethanol aqueous solution, and purifying an extracting solution with an HP-20 macroporous adsorption resin column. All the components are synergistically used, a multifunctional integrated repair system is constructed, and the problems that a traditional medicine is insufficient in biocompatibility, single in function and low in repair efficiency are solved.

The invention discloses a chloramphenicol hapten, antigen, antibody and colloidal gold detection device as well as a preparation method and application thereof. According to the chloramphenicol hapten prepared by using a total synthesis method, all potential recognition sites of chloramphenicol are reserved to the maximum extent, particularly, the most critical dichloro structure of chloramphenicol is reserved for the first time, after a spacer arm is introduced, the distribution of electron cloud of the hapten is almost consistent with that of overall electron cloud of chloramphenicol, and the immunogenicity of the chloramphenicol antigen is improved; the chloramphenicol antigen and the monoclonal antibody prepared by the invention are high in specificity when being used for ELISA detection, the IC50 value is 8.21 pg / mL, the linear detection range is 1.71-39.45 pg / mL, and the cross reaction rates of the chloramphenicol antigen and the monoclonal antibody to common structural analogues are lower than 0.01%; and the sensitivity to chloramphenicol in a sample is 0.1 g / kg.

The invention discloses a method for synchronously separating ovalbumin, ovotransferrin and lysozyme from egg white, which comprises the following steps: firstly, adsorbing ovalbumin from egg white loading liquid by taking chitosan-polybutyl methacrylate nanocrystal gel with an anion exchange function as a primary separation medium, and carrying out gradient elution to obtain ovalbumin; taking a primary separation loading effluent as a secondary separation loading solution; hydroxyethyl methylacrylate-polyglycidyl methacrylate nanocrystal gel with a cation exchange function is taken as a secondary separation medium to adsorb ovotransferrin and lysozyme from a secondary separation loading solution, gradient elution is performed by gradually increasing salt concentration, and separation of ovotransferrin and lysozyme is realized. The method provided by the invention can realize synchronous separation of ovalbumin, ovotransferrin and lysozyme, has the advantages of high separation efficiency, simplicity and convenience in operation, low energy consumption and the like, and is suitable for industrial large-scale production, and the separation medium can be repeatedly used.

The invention relates to a chlorantraniliprole hapten, a complete antigen and application of the chlorantraniliprole hapten and the complete antigen. When the chlorantraniliprole artificial antigen and the monoclonal antibody are used for ELISA detection, the specificity is high, the IC50 value is 0.058 [mu] g / L, the detection limit of chlorantraniliprole in a standard solution reaches 0.6 [mu] g / L, and the detection limit of a sample to be detected reaches 2 g / kg; the cross reaction rates with cyantraniliprole, tetrachlorantraniliprole and cyclaniliprole are respectively 20%, 12% and 12%, and the material specificity is greatly improved. By utilizing the chlorantraniliprole hapten, the complete antigen and the antibody, the purpose of rapidly and accurately detecting chlorantraniliprole in a sample can be achieved.

This invention relates to a cicutine hapten, a hybridoma cell line, an antibody, and their applications, belonging to the field of immunochemistry. The hybridoma cell line, ISO 400, is deposited at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, on April 18, 2024, with accession number CGMCC No. 45918. The monoclonal antibody secreted by the cell line provided by this invention exhibits good detection sensitivity (IC50) for cicutine. 50 With a value of 9.69 μg / mL and high specificity, it enables rapid detection of coniine, providing raw materials for the immunoassay of coniine in hemlock and cosmetics, and has practical application value.

Provided herein are compositions with enhanced protein content, compositions with functional proteins, protein combinations and methods for the preparation thereof.

The application discloses a redox response carrier protein and application thereof in preparation of vaccines, wherein at least one hapten structure is chemically coupled and modified on the carrier protein through a connecting arm containing a disulfide bond structure, and the hapten structure is selected from one of dinitrobenzene, galactose-alpha-(1,3)-galactose, rhamnose and phosphorylcholine. The redox response disulfide bond connecting arm can modify the hapten to the carrier protein, and can be recognized and combined by the naturally existing antibody in the body to form a complex, promote phagocytosis, processing and presentation of antigen presenting cells, thereby effectively improving the immunogenicity of the vaccine molecule and improving the antigen related immune response. After the redox disulfide bond connecting arm enters the lysosome, the disulfide bond is broken through response to the reducing property in the cell, lysosome escape occurs, cross-layer delivery of the antigen is realized, and the immunogenicity of the antigen is greatly improved.

The present application relates to a kind of hybridoma cell strains secreting vitamin B3 monoclonal antibody and its application, belong to immune detection technical field.The present application has obtained a kind of hybridoma cell strains capable of secreting vitamin B3 monoclonal antibody after screening many times, the vitamin B3 monoclonal antibody secreted by the hybridoma cell strains of the present application has excellent affinity and sensitivity to vitamin B3, the IC 50 It reaches 14.36 ng / mL, and for the structural analog of vitamin B3, such as vitamin A, vitamin K2, vitamin K3, vitamin K4, vitamin E, there is no cross reaction.Therefore the monoclonal antibody of the present application can be used for preparing the immune detection product of vitamin B3, provides efficient detection method and means for the residual detection of vitamin B3.

This invention discloses a pyrimethanil hapten, an artificial antigen, and their preparation methods and applications. The structural formula of the pyrimethanil hapten is shown below. The arm introduced in this invention not only possesses an active group but also fully retains the benzene ring and dimethylpyrimidine ring structure of the target analyte, enhancing the recognition site. The pyrimethanil artificial antigen and monoclonal antibody of this invention exhibit high specificity for ELISA detection, with an IC50 rating of [missing information]. 50 The value is 1.97 μg / L, which is better than existing monoclonal antibodies. It can be used in colloidal gold immunochromatography to rapidly and conveniently detect pyrimethanil. The colloidal gold immunochromatographic test strip prepared in this invention has a detection sensitivity of 10 μg / L for pyrimethanil.

The invention discloses a method for preparing low-sensitization protein peptide through microwave combined enzymolysis composite modification and application, and relates to the technical field of food processing. The treatment method comprises the following steps: preparing protein powder into a solution, adding tea polyphenol into the solution, and uniformly mixing to prepare a protein solution; carrying out microwave modification treatment on the protein solution; adding compound protease into the protein solution subjected to microwave treatment for enzymolysis and enzyme deactivation; and filtering the enzymolysis product, and drying the filtrate to obtain the low-sensitization protein peptide. According to the method, microwave and enzymolysis combined composite modification is adopted, so that the sensitization of protein can be reduced, the reaction time is shortened, and the method is simple in process, free of pollution and high in safety. In the enzymolysis process, active peptide substances which are easy to digest and absorb, can enhance immunity and delay senescence are synchronously generated, and tea polyphenol added in the formula can synergistically relieve anaphylactic reaction and play an anti-oxidation role, so that multi-element synergy of desensitization, function enhancement and flavor optimization is realized, and the added value and market application potential of the product are improved.

The invention relates to the technical field of immunoassay, and provides a vomitoxin hapten, an antigen, an antibody, a preparation method and a light-activated chemiluminescence quantitative detection kit. The hapten is obtained by adopting cyclic dicarboxylic anhydride as a reactant and introducing an active group on a C atom of conjugated alkene of vomitoxin molecules through Friedel-Crafts acylation reaction. After the hapten is coupled with a carrier protein, vomitoxin molecules can be fully exposed outside the carrier protein, the immunogenicity of the antigen is enhanced, and an antibody with high titer and high sensitivity can be generated after the hapten is immunized by a host animal. Results of the embodiment show that after the vomitoxin antigen is subjected to mouse injection immunization, the titer of the generated antibody is 1: 20000, and the half inhibitory concentration (IC50) is less than 50ppb / mL. When the obtained vomitoxin antigen and antibody are applied to rapid quantitative detection of light-activated chemiluminescence of vomitoxin, the detection limit, the false negative rate and other dimensions are qualified, the detection steps are simple, and the vomitoxin antigen and antibody have good industrial value.

The application discloses an oxycarbamazepine content detection hapten and application thereof, and particularly relates to an oxycarbamazepine content detection hapten and a preparation method thereof, and further discloses application of the hapten in antibodies and kits. The enzyme-linked immunoassay kit established for the oxycarbamazepine content detection hapten can realize rapid detection of a large number of samples to be detected, is convenient to use, low in detection cost, efficient, accurate, rapid, can simultaneously detect a large number of samples, and is suitable for on-site monitoring and screening of a large number of samples of oxycarbamazepine in agricultural products.

This invention discloses chloramphenicol hapten, antigen, antibody, colloidal gold detection device, and their preparation methods and applications. The chloramphenicol hapten prepared by this invention using a total synthesis method retains all potential recognition sites of chloramphenicol to the maximum extent, and in particular, for the first time retains the most critical dichloro structure of chloramphenicol. After introducing a spacer arm, its electron cloud distribution is almost identical to the overall electron cloud distribution of chloramphenicol, improving the immunogenicity of the chloramphenicol antigen. The chloramphenicol antigen and monoclonal antibody prepared by this invention have high specificity for ELISA detection, with an IC50 value of [missing information]. 50 The value was 8.21 pg / mL, the linear detection range was 1.71–39.45 pg / mL, the cross-reactivity to common structural analogs was less than 0.01%, and the sensitivity to chloramphenicol in the sample was 0.1 µg / kg.

The invention belongs to the technical field of food and medicine safety detection, and relates to a hybridoma cell strain secreting an anti-tert-butylhydroquinone monoclonal antibody and application thereof. The hybridoma cell strain secreting the anti-monoclonal antibody is preserved in the China General Microbiological Culture Collection Center on April 17, 2025, the preservation number is CGMCC No.46514, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the hybridoma cell strain is classified and named as a monoclonal cell strain. The monoclonal antibody cell strain for the tert-butylhydroquinone obtained by the invention can be used for detecting the residue of the tert-butylhydroquinone in food, has better detection sensitivity and specificity on the tert-butylhydroquinone, and can be used for detecting the tert-butylhydroquinone in the food; an efficient immunodetection tool is provided for safety monitoring of food additives, and the kit has a definite practical application value.

Provided herein are compositions, proteins, polynucleotides, expression vectors, host cells, kits, and systems for producing egg white proteins, as well as methods of using the same.

This invention belongs to the field of immunoassay technology, specifically relating to acetylpyridinium hapten, complete antigen, antibody, preparation methods, and applications. The acetylpyridinium hapten provided by this invention has the structure shown in Formula I, where R1 is an active group capable of direct coupling with a carrier protein. The acetylpyridinium hapten provided by this invention retains the basic structure of acetylpyridinium, using the original -CH2-CH2-CH2-CH2- structure of acetylpyridinium as a linker arm. An active group R1 capable of direct coupling with a carrier protein is introduced into the linker arm at a site far from the characteristic structure of acetylpyridinium, effectively avoiding non-specific binding caused by the external linker arm. After coupling with the carrier protein, the introduced active group can fully expose the characteristic structure of the acetylpyridinium bulk molecule. The prepared acetylpyridinium complete antigen is immunogenic, and the prepared anti-acetylpyridinium antibody has good specificity and high sensitivity. It can be used to establish immunological detection methods or reagents for acetylpyridinium, and has broad application prospects.

This disclosure provides expression systems comprising secretion signals that promote production and / or secretion of proteins of interest, as well as one or more polynucleotides encoding chaperone proteins (e.g., CRT and / or PDIA3) which, as demonstrate herein, enhance production and / or secretion of proteins. Moreover, genetically modified host cells comprising these expression systems are capable of producing high levels of protein of interest, such as bovine lactoferrin (bLF), bovine lactoglobulin (bLG), or ovalbumin (Ova).