86 results about "Homocysteine" patented technology

The invention belongs to the technical field of biological detection, and particularly relates to a method for synchronously and quantitatively detecting various folic acid metabolism related substances in trace plasma or serum, which comprises the following steps of: detecting a pretreated trace plasma or serum sample by adopting a liquid chromatography-tandem mass spectrometry method; the following nine folic acid metabolism related substances are synchronously and quantitatively detected: 5-formyl tetrahydrofolic acid, folic acid, 5-methyl tetrahydrofolic acid, S-adenosine-L-methionine, S-adenosine-L-homocysteine, vitamin B12, pyridoxamine, pyridoxine and pyridoxal. The invention further relates to application of the detection method in related detection of child neurodevelopment. According to the detection method, only trace plasma or serum is used, methotrexate is used for replacing an expensive isotope internal standard for more economical detection, and nine folate metabolism related substances can be accurately quantified at the same time. Therefore, the detection method disclosed by the invention has important clinical significance and wide market application prospect.

The invention discloses an application of a traditional Chinese medicine composition in preparation of a medicine for preventing and treating atherosclerosis combined with hyperhomocysteinemia and / or hyperlipidemia. The traditional Chinese medicine composition comprises the following components: ginseng, lucid ganoderma, safflower, musk, calculus bovis, bear gall, pearl, venenum bufonis, radix aconiti carmichaeli and borneol. According to the traditional Chinese medicine composition disclosed by the invention, through reasonable compatibility of all the components, at least one of indexes such as aorta plaque area, vasomotor inner diameter difference, intima-media thickness, blood fat level, homocysteine content and the like in atherosclerosis combined with hyperhomocysteinemia and / or hyperlipidemia can be effectively improved; the symptoms of atherosclerosis combined with hyperhomocysteinemia and / or hyperlipidemia are relieved from multiple aspects, and the progress of the disease course is blocked. The traditional Chinese medicine composition is controllable in quality, convenient for standardized production, safe and reliable in prescription and free of toxic and side effects on human bodies.

The invention discloses an acryloyl-based fluorescent probe with a camphor derivative structure, which takes a camphor derivative as a fluorophore and acryloyl as a recognition group, and has a structural formula as shown in (I): # imgabs0. The probe molecule emits orange fluorescence in a solution, and after the probe molecule responds to cysteine (Cys), the solution fluorescence is quenched; after response with homocysteine (Hcy) / glutathione (GSH), solution fluorescence is enhanced, and yellow fluorescence is emitted. The fluorescent probe disclosed by the invention not only can be used for detecting biological mercaptan in a water sample and a biological sample, but also can be used for distinguishing cysteine from homocysteine / glutathione. The fluorescent probe provided by the invention has very high sensitivity, good selectivity and anti-interference performance, can rapidly distinguish biological mercaptan, shortens response time, and realizes real-time effective detection of biological mercaptan. The fluorescent probe disclosed by the invention can realize biological mercaptan imaging in cells and organisms, is expected to play a role in industrial production, food detection and clinical medicine detection, and has a wide application prospect.

The invention relates to a detection kit for combined methylmalonemia, which is a cb1C type kit and comprises a specific primer pair and a probe combination for detecting at least one mutation site of MMACHC, the mutation site is selected from c.609Ggt; a is c.567 dupT, c.658660delAAG, c.482Ggt, and c.482Ggt; a, c.1Agt; g, c, 80Agt; g, c, 217Cgt; 315Cgt, T, c.315Cgt; g and c.394 Cgt; t). The MMACHC gene hotspot mutation region detection kit based on the real-time fluorescent quantitative PCR technology can detect multiple mutation sites on the MMACHC gene at the same time, detection of three genotypes of wild type, homozygous mutant type and heterozygous mutant type on each mutation site is completed, the gene detection requirements of clinical combined type MMA are effectively met, the detection time is shortened, and the detection efficiency is improved. The mutation site coverage rate of CblC defective methylmalonic acidmia combined with homocysteinemia on the MMACHC gene is increased to 93.28%, mutation hot spots of most people are covered, and various types of mutation conditions in hot spot mutation areas can be rapidly, accurately and sensitively detected.

The present invention provides a homocysteine ​​electrochemical sensor, and its preparation method and application. The preparation method of the homocysteine ​​electrochemical sensor of the present invention comprises the following steps: adding a dispersion containing carbon nanomaterials to the surface of a glassy carbon electrode, baking and drying, to obtain a carbon nanomaterial-modified electrode; then placing the carbon nanomaterial-modified electrode in a gold salt aqueous solution, and using an electroplating method to deposit nanogold on the surface of the carbon nanomaterial-modified electrode, to obtain a homocysteine ​​electrochemical sensor; the high conductivity of gold nanoparticles is of great significance for improving the electrocatalytic performance of the composite material for homocysteine. The homocysteine ​​electrochemical sensor prepared by the present application has low cost, low detection limit, and good stability. In addition, the spiked recovery rate of homocysteine ​​in serum samples is good, which provides potential application value for the reliable determination of homocysteine ​​in biological systems and the study of homocysteine-related diseases.

The invention discloses a method for detecting homocysteine by a nano-pore sensor based on silver-sulfur polymerization, which comprises the following steps: pretreating a solution containing the homocysteine by 4-dimethylamino cinnamyl aldehyde, and then putting a sensor with a cylindrical nano-pore structure into the solution containing the homocysteine to carry out polymerization reaction; establishing a linear equation of the ratio of the conductivity before and after the polymerization reaction and the concentration of the homocysteine, and calculating the concentration of the homocysteine in the liquid to be detected through the linear equation. The liquid to be detected contains homocysteine and cysteine, and is pretreated by 4-dimethyl amino cinnamyl aldehyde before detection; and an Ag (I) probe is modified in a cylindrical nanopore channel of the sensor. According to the method, a special pretreatment method is utilized to cooperate with a constructed functional probe and a special nanopore structure, and current signal change is amplified based on silver-sulfur polymerization reaction, so that rapid and accurate detection of Hcy is realized.

The invention discloses a radiation enteritis differential metabolite marker based on faeces metabonomics and a screening method and application of the radiation enteritis differential metabolite marker. The radiation proctitis differential metabolite marker is prepared from (R)-lipoic acid salt, (S)-malic acid, 3-mercaptolactic acid, 4-acetaminobutyric acid, 4-guanidinobutyric acid, 5-hydroxyindoleacetic acid, 5-methoxy indoleacetic acid, isocitrate, L-homocysteine and L-proline. The excrement differential metabolite is used as the radiation proctitis biomarker, has important significance on clinical early diagnosis of radiation proctitis, and can be used for preparing a diagnosis tool for radiation proctitis, predicting the individual treatment effect of radiation proctitis patients and preparing a medicine for treating radiation proctitis.

The invention provides a homocysteine hapten as well as a preparation method and application thereof. The hapten is prepared from L-homocysteine through a coupling reaction of sulfydryl and amino of 2, 4, 5-triamino-6-chloropyrimidine, and the hapten is obtained through a coupling reaction of sulfydryl and amino of 2, 4, 5-triamino-6-chloropyrimidine. The hapten is coupled with a complete antigen formed by carrier protein. The antigen can form strong immunoreaction and is beneficial to screening of specific Hcy antibodies.

The invention provides a method for separating and detecting components in butanedisulfonic acid ademetionine enteric-coated tablets, and belongs to the technical field of medicine quality analysis. According to the method, ammonium formate solution-water-acetonitrile and ammonium formate solution-acetonitrile-methyl tert-butyl ether are used as a mobile phase system, and the pH value of the ammonium formate solution is controlled to be 2.2-3.1, so that adenosine and adenine can be effectively separated; according to the method, the S-adenosyl-L-methionine glycine isomer 2, adenosine, adenine and S-adenosyl-L-homocysteine can be separated, the time consumption is short, the separation efficiency is high, the operation is simple, the separation cost is low, and the quality control of butanedisulfonic acid adenosyl methionine or related preparations can be improved. According to the method, the detection of the ademetionine and the related impurities is not influenced by auxiliary materials, the method is efficient and convenient, good in reproducibility, high in specificity, high in accuracy and high in sensitivity, and the quality control of the ademetionine sample can be realized.

The present invention relates to a lubricating fluid, preferably water-based, comprising: a) a sulphur-containing compound or one of the salts thereof, more particularly homocysteine; b) a polyalkylene glycol; c) water; and d) optionally one or more functional additives. The present invention also relates to the use of this lubricating fluid for lubricating and / or cleaning mechanical devices, for metalwork, as a hydraulic fluid, or as a fluid for mining operations. The present invention also relates to a method for lubricating a metal or a composite, the method comprising bringing the metal or the composite into contact with the lubricating fluid.

The invention discloses a clinical prediction model for the dyslipidemia probability of a child hepatolenticular degeneration patient and a design method, and belongs to the technical field of medical information. The clinical prediction model comprises an input module for receiving patient parameter indexes consisting of an age group (Age group, lt, 10 years old or more), alanine aminotransferase (ALT), gamma-glutamyltransferase (GGT), homocysteine (Hcy), superoxide dismutase (SOD) and platelet count (PLT); the processing module is connected with the input module and calculates the prediction probability of the patient according to the model, and the output module is connected with the processing module and outputs the probability of dyslipidemia of the child hepatolenticular degeneration patient, which is finally obtained by the clinical prediction model. The prediction model established by the invention has good distinction degree, calibration degree and clinical practicability, has relatively high accuracy, and can be conveniently used for early recognition and risk prediction of dyslipidemia caused by hepatolenticular degeneration of children.

Inventors describe herein the functional and structural characteristics of the MGL from the anaerobic alkaliphilic thermophile Thermobrachium celere (TcMGL), which was compared to that from Pseudomonas putida (PpMGL). Catalysis was limited to L-Met, L-homocysteine, and L-cysteine, with the highest catalytic efficiency (keat / KM) towards L-Met. The half-life of active TcMGL in human plasma at 37°C was 9.2 hour. Half-life increased from 11.3 hour to 66.7 hour with increasing PLP concentration from 2 to 200 µM, in much greater magnitude than in PpMGL case. TcMGL crystal structure resolution revealed that the enzyme is a tetramer of two catalytic dimers containing a mobile region with open and closed conformations resulting in differences for access to the active site. TcMGL possesses favourable characteristics which makes it a suitable candidate for treatment of cancer. The present invention relates to a polypeptide comprising an amino acid sequence as set forth by SEQ ID NO: 1 or a function-conservative variant thereof.

The invention relates to the field of intelligent medical treatment, in particular to a pregnancy disease risk prediction method and device based on biochemical indexes and a program product. Comprising the following steps: S1, acquiring serum biochemical index data of a pregnant woman; s2, comparing the serum biochemical indexes with a preset threshold value; the serum biochemical indexes include aspartate aminotransferase, total protein, albumin, lactic dehydrogenase, alkaline phosphatase, total bile acid, creatine kinase, blood sugar, urea nitrogen, uric acid, creatinine, glomerular filtration rate, potassium, chlorine, total calcium, serum magnesium, inorganic phosphorus, total cholesterol, triglyceride, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol and homocysteine. When one or more of the myoglobin, the hypersensitive troponin I, the prealbumin, the cystatin C and the creatine kinase isoenzyme MB are abnormal, judging that the pregnancy eclampsia exists. The method is simple, convenient and rapid to operate and low in cost, can be clinically popularized to predict the health state of the pregnant woman on a large scale, and has good clinical value.

The invention discloses an amine-terminated acrylamide polymer shale inhibitor and a preparation method thereof. Polymeric monomers of the inhibitor comprise an acrylamide monomer A, N, N-dimethylacrylamide, acryloyl morpholine and an amino thiol monomer. The acrylamide monomer A is selected from at least one of methacrylamide, isopropyl acrylamide and acrylamide; the amino thiol monomer is selected from at least one of cysteine and homocysteine. The synthesis process is simple and efficient, the cost is low, and the prepared polyamine inhibitor is high in shale hydration inhibition, stable in performance under the high-temperature condition and good in compatibility.

The present invention provides a device for detecting an analyte in a liquid sample, the device comprising: a test element for testing whether the analyte exists in the liquid sample or the number of the analyte, the analyte being one or more of the following analyte: neopterin; the amino acid sequence is shown in the description, and the amino acid sequence is shown in the description. The invention relates to a preparation method of a graphene oxide-dopamine composite material. The invention also relates to a method for preparing the microalbuminuria. The microalbuminuria is prepared from the microalbuminuria.

A method is disclosed for chemo-enzymatic production of the functionally active stereoisomer of S-adenosyl-L-methionine analogs with extended transferable moieties of formula (I) and (II) from corresponding alkylbromides and S-adenosyl-L-homocysteine.

The invention discloses an application of an aldehyde group functionalized iridium (III) three-heterocomplex ionic complex phosphorescent probe in detection of biological mercaptan molecules, and belongs to the technical field of biological mercaptan sensing. The aldehyde group functionalized iridium (III) three-heteroligand ionic complex phosphorescent probe is self-assembled through coordination of three functional complementary ligands and an iridium (III) metal center, the probe has visible and near-infrared emission characteristics, and through the synergistic effect of excited state ligand-metal charge transfer and thiol specific nucleophilic addition reaction, the fluorescence intensity of the probe is improved, and the fluorescence intensity of the probe is improved. The high-selectivity phosphorescent recognition of sulfur-containing amino acids such as cysteine (Cys) and homocysteine (Hcy) is realized, the practicability and convenience of the probe are improved, and a new technical thought and method are provided for the fields of biological imaging and molecular detection. The probe has a wide application prospect in the fields of tumor diagnosis, drug screening, environment monitoring, food safety detection and the like.

The invention discloses an Escherichia coli strain capable of efficiently secreting and expressing alpha-lipoic acid and application of the Escherichia coli strain, and belongs to the technical field of biology. Escherichia coli is used as a chassis strain for synthesizing lipoic acid by a microbiological method, and an efficient production strain is constructed by optimizing a metabolic pathway; the related modification comprises the following steps: overexpressing a lipoic amidase coding gene eflpA, a truncated thioesterase mutant 'TesA coding gene, a lipoyl synthase coding gene lipA, an iron-sulfur cluster regeneration gene cluster coding gene iscSUA, an S-adenosylmethionine synthetase coding gene sam2 and a 5'-methyl adenosine thioate / S-adenosylhomocysteine nucleoside enzyme coding gene mtn, and carrying out gene coding. A fatty acid degrading enzyme coding gene fadE, a thioesterase coding gene tesA, a pyruvate formate lyase gene cluster coding gene pflAB and a glucokinase coding gene glk are knocked out. According to the invention, the Escherichia coli realizes efficient de novo synthesis and secretory expression of lipoic acid, and finally the extracellular yield in the shake flask fermentation process reaches 16.96 mg / L.

The application provides a method for separating and detecting ingredients in a butanedisulfonic acid ademetionine enteric-coated tablet, and belongs to the technical field of drug quality analysis. The application uses ammonium formate solution-water-acetonitrile and ammonium formate solution-acetonitrile-methyl tert-butyl ether as a mobile phase system, and controls the pH value of the ammonium formate solution to be 2.2-3.1, so that adenosine and adenine can be effectively separated, and ademetionine, decarboxylated ademetionine, S-adenosyl-L-methionine glycine isomer 2, adenosine, adenine and S-adenosyl-L-homocysteine can be highly separated at one time, the method has the advantages of short time consumption, high separation efficiency, simple operation, low separation cost, and is favorable for improving the quality control of butanedisulfonic acid ademetionine or related preparations. In the application, the detection of ademetionine and related impurities is not affected by excipients, and has the advantages of high efficiency, convenience, good reproducibility, strong specificity, high accuracy and high sensitivity, and can realize the quality control of ademetionine samples.

The invention discloses a pharmaceutical composition containing phosphobetaine, a preparation method and application of the pharmaceutical composition in preparing a medicine for treating methylmalonic acidemia combined with homocystinemia, belonging to the technical field of betaine. The preparation method comprises the following steps: preparing phosphobetaine and compounding; the compounding comprises the following steps: preparing calcium hydroxycarbonate, compounding once, compounding twice and coating; the pharmaceutical composition containing phosphobetaine of the invention can ensure the therapeutic effect on methylmalonic acidemia combined with homocystinemia, and the preparation method of the pharmaceutical composition containing phosphobetaine of the invention can also improve the purity and yield of phosphobetaine, avoid agglomeration of phosphobetaine crystals, and the prepared pharmaceutical composition containing phosphobetaine has weak moisture absorption, strong fluidity and good high temperature resistance.

The invention discloses a preparation method of an antibacterial epoxy resin coating, and relates to the technical field of coatings. According to the invention, the 5-phenylpent-2, 4-dienoic acid is esterified and combined with the side chain of the epoxy resin, so that the hardness and heat resistance of the cured coating are improved; then addition reaction with dimethyl diallyl ammonium chloride, 2-acrylamide phenylboronic acid and the modified antibacterial agent is performed to form a three-dimensional network structure, so that the mechanical strength of the coating is remarkably enhanced, the antibacterial, high-adhesion and anti-stripping effects are achieved, a phenylboronic acid structure is assisted, a protective film is formed on the surface of an object, the object is effectively isolated from the external environment, and the service life of the coating is prolonged. Oxidation and corrosion of the object surface are prevented, and the corrosion-resistant effect is achieved. According to the modified antibacterial agent, S-adenosine-L-homocysteine and silver nitrate are subjected to a coordination reaction, and the antibacterial effect is achieved; then, the silicon dioxide is combined with vinyl trichlorosilane, silane groups are introduced, after curing, the mechanical strength and water resistance of the coating are remarkably enhanced, corrosive liquid is prevented from entering the coating, and the corrosion-resistant effect is indirectly improved.

The present invention discloses a preparation method, product and application of a triple artificial mimic enzyme, relating to the technical field of biological detection. The preparation method comprises the following steps: (1) Mixing a HAuCL4 solution and a CTAB solution, and then adding a NaBH4 solution to obtain a seed solution of gold nanorods; (2) After mixing a HAuCL4 solution and a cetyltrimethylammonium bromide solution, adding an AgNO3 solution and H-RGO, then adding a reducing agent, and finally adding the seed solution of the gold nanorods, and reacting to obtain GO-Au; (3) Connecting MP-11 to the GO-Au to obtain the triple artificial mimic enzyme. The present invention designs and synthesizes a triple artificial mimic enzyme based on graphene, MP-11 and gold nanoflowers. This artificial mimic enzyme can replace natural peroxidase to highly sensitively detect the concentration of homocysteine in human plasma.

A method for identifying pre-disposition to cognitive decline in a subject, the method comprising determining levels of: (a) omega-3 fatty acids, and vitamin D or a metabolite thereof; (b) omega-3 fatty acids, and homocysteine; (c) vitamin D or a metabolite thereof, and homocysteine; or (d) omega-3 fatty acids, vitamin D or a metabolite thereof, and homocysteine, independently in one or more samples obtained from the subject.

The invention discloses a gastrointestinal tumor patient postoperative weakness risk correlation analysis method and system fused with a co-disease map, and belongs to the field of medical care informatics, and the method comprises the following steps: obtaining clinical pathological characteristics, co-disease assessment, serum biomarkers and social and psychological assessment scale data of gastrointestinal tumor patients; obtaining statistical effect values of homocysteine and health attainment, and executing isomorphic mapping; identifying a chained intermediary conduction path and generating a coupling strength factor, and performing weighted correction on the initialized weight matrix; inputting the multi-source data as a risk source excitation signal into the atlas, executing correction calculation, and outputting a risk prediction score; a maximum weight subgraph search is performed to generate risk attribution analysis data. According to the method, the multi-dimensional heterogeneous association map is constructed to fuse the multi-source data of the patient and the medical priori knowledge, and the risk conduction is quantified through the path topology analysis and the matrix correction operation, so that the accurate prediction of the postoperative weakness risk and the interpretable attribution of the key pathogenic path can be realized.

The invention discloses a strain of Weizmania coagulans AUSA001 which is separated from human milk and an application of the strain of Weizmania coagulans AUSA001. The bacillus coagulans AUSA001 provided by the invention is preserved in Guangdong Microbial Culture Collection Center, the preservation date is March 15, 2024, and the preservation number is GDMCC.No.64416. The bacillus coagulans AUSA001 provided by the invention has the advantages that the bacillus coagulans AUSA001 is preserved in the Guangdong Microbial Culture Collection Center; the Witzia ciliaris AUSA001 provided by the invention can be used for preparing a preparation for reducing homocysteine and preventing and treating cerebral apoplexy.

The invention provides a synthesis method of S-allyl sulfydryl cysteine (SAMC), S-allyl sulfydryl-glutathione (GSSA) and S-allyl sulfydryl-homocysteine (SAMC). The invention further provides a preparation method of the S-allyl sulfydryl-glutathione (GSSA) and the S-allyl sulfydryl-homocysteine (SAMC). Specifically, allicin generated by reaction of alliin and allinase is taken as a substrate and reacts with cysteine (Cys), glutathione (GSH) and homocysteine (Hcy) to generate SAMC, GSSA and SAMHC. According to the method, high-purity SAMC, high-purity GSSA and high-purity SAMHC can be obtained, and the yield is high.

The invention belongs to the technical field of electrochemical analysis methods, and particularly relates to a copper nanowire-coated Py-PB-COF compound modified electrode, a preparation method of the copper nanowire-coated Py-PB-COF compound modified electrode and a detection method of homocysteine. The preparation method comprises the following steps: uniformly mixing and grinding a copper nanowire dispersion liquid and a Py-PB-COF material to obtain a copper nanowire-coated Py-PB-COF composite material, mixing and grinding the copper nanowire-coated Py-PB-COF composite material, nano graphite powder and paraffin oil, and self-assembling to prepare the compound modified electrode, and the method is simple and easy to operate. When the prepared compound modified electrode is applied to detection of homocysteine, the linear range is wider, the sensitivity and the selectivity are good, and the reproducibility and the stability are good.

The present invention provides a method for detecting cancer of a digestive organ in a subject, the method including a step for measuring the amount of cysteine persulfide or homocysteine persulfide in a sample derived from the subject, wherein the sample is derived from a tissue other than a digestive organ tissue, which is suspected of being affected by cancer, of the subject. The present invention also provides a method for diagnosing cancer of a digestive organ and a method for treating a digestive organ, in which the above-mentioned method is employed.

The invention provides a pharmaceutical composition. The pharmaceutical composition is prepared from amlodipine with the minimum hypertension treatment dosage of 20%-50%, valsartan with the minimum hypertension treatment dosage of 20%-50%, metoprolol with the minimum hypertension treatment dosage of 20%-50%, hydrochlorothiazide with the minimum hypertension treatment dosage of 20%-50%, homocysteine reducing medicine and pharmaceutically acceptable auxiliary materials. The pharmaceutical composition is used for treating hypertension, is especially suitable for hypertension with heart failure, and can reduce the death risk of patients.

The invention belongs to the technical field of synthetic biology, and particularly relates to a genetically engineered bacterium for high yield of L-methionine as well as a construction method and application of the genetically engineered bacterium. By strengthening the hemC gene, the utilization capacity of escherichia coli on a sulfur source is improved; by strengthening the malY gene and the multi-copy metB gene, the capability of converting cysteine into homocysteine in the escherichia coli is improved; by knocking out an mnaT gene and introducing metA to a genome to resist feedback, the degradation of L-methionine is reduced, and meanwhile, the supply of a precursor O-succinyl homoserine is increased; and finally, a pAmZK plasmid is introduced to obtain the escherichia coli genetically engineered bacterium strain with high yield of L-methionine, and the yield is increased from 3.21 g / L to 3.67 g / L. And fed-batch fermentation is carried out in a 5L tank in a pH feedback feeding manner, the yield reaches 43.55 g / L within 64 hours, and a research foundation is laid for production of L-methionine by a microbiological method.