47 results about "Homocysteine" patented technology

The invention belongs to the technical field of biological detection, and particularly relates to a method for synchronously and quantitatively detecting various folic acid metabolism related substances in trace plasma or serum, which comprises the following steps of: detecting a pretreated trace plasma or serum sample by adopting a liquid chromatography-tandem mass spectrometry method; the following nine folic acid metabolism related substances are synchronously and quantitatively detected: 5-formyl tetrahydrofolic acid, folic acid, 5-methyl tetrahydrofolic acid, S-adenosine-L-methionine, S-adenosine-L-homocysteine, vitamin B12, pyridoxamine, pyridoxine and pyridoxal. The invention further relates to application of the detection method in related detection of child neurodevelopment. According to the detection method, only trace plasma or serum is used, methotrexate is used for replacing an expensive isotope internal standard for more economical detection, and nine folate metabolism related substances can be accurately quantified at the same time. Therefore, the detection method disclosed by the invention has important clinical significance and wide market application prospect.

The present invention relates to a lubricating fluid, preferably water-based, comprising: a) a sulphur-containing compound or one of the salts thereof, more particularly homocysteine; b) a polyalkylene glycol; c) water; and d) optionally one or more functional additives. The present invention also relates to the use of this lubricating fluid for lubricating and / or cleaning mechanical devices, for metalwork, as a hydraulic fluid, or as a fluid for mining operations. The present invention also relates to a method for lubricating a metal or a composite, the method comprising bringing the metal or the composite into contact with the lubricating fluid.

The invention discloses a clinical prediction model for the dyslipidemia probability of a child hepatolenticular degeneration patient and a design method, and belongs to the technical field of medical information. The clinical prediction model comprises an input module for receiving patient parameter indexes consisting of an age group (Age group, lt, 10 years old or more), alanine aminotransferase (ALT), gamma-glutamyltransferase (GGT), homocysteine (Hcy), superoxide dismutase (SOD) and platelet count (PLT); the processing module is connected with the input module and calculates the prediction probability of the patient according to the model, and the output module is connected with the processing module and outputs the probability of dyslipidemia of the child hepatolenticular degeneration patient, which is finally obtained by the clinical prediction model. The prediction model established by the invention has good distinction degree, calibration degree and clinical practicability, has relatively high accuracy, and can be conveniently used for early recognition and risk prediction of dyslipidemia caused by hepatolenticular degeneration of children.

Inventors describe herein the functional and structural characteristics of the MGL from the anaerobic alkaliphilic thermophile Thermobrachium celere (TcMGL), which was compared to that from Pseudomonas putida (PpMGL). Catalysis was limited to L-Met, L-homocysteine, and L-cysteine, with the highest catalytic efficiency (keat / KM) towards L-Met. The half-life of active TcMGL in human plasma at 37°C was 9.2 hour. Half-life increased from 11.3 hour to 66.7 hour with increasing PLP concentration from 2 to 200 µM, in much greater magnitude than in PpMGL case. TcMGL crystal structure resolution revealed that the enzyme is a tetramer of two catalytic dimers containing a mobile region with open and closed conformations resulting in differences for access to the active site. TcMGL possesses favourable characteristics which makes it a suitable candidate for treatment of cancer. The present invention relates to a polypeptide comprising an amino acid sequence as set forth by SEQ ID NO: 1 or a function-conservative variant thereof.

The invention discloses an amine-terminated acrylamide polymer shale inhibitor and a preparation method thereof. Polymeric monomers of the inhibitor comprise an acrylamide monomer A, N, N-dimethylacrylamide, acryloyl morpholine and an amino thiol monomer. The acrylamide monomer A is selected from at least one of methacrylamide, isopropyl acrylamide and acrylamide; the amino thiol monomer is selected from at least one of cysteine and homocysteine. The synthesis process is simple and efficient, the cost is low, and the prepared polyamine inhibitor is high in shale hydration inhibition, stable in performance under the high-temperature condition and good in compatibility.

The application provides a method for separating and detecting ingredients in a butanedisulfonic acid ademetionine enteric-coated tablet, and belongs to the technical field of drug quality analysis. The application uses ammonium formate solution-water-acetonitrile and ammonium formate solution-acetonitrile-methyl tert-butyl ether as a mobile phase system, and controls the pH value of the ammonium formate solution to be 2.2-3.1, so that adenosine and adenine can be effectively separated, and ademetionine, decarboxylated ademetionine, S-adenosyl-L-methionine glycine isomer 2, adenosine, adenine and S-adenosyl-L-homocysteine can be highly separated at one time, the method has the advantages of short time consumption, high separation efficiency, simple operation, low separation cost, and is favorable for improving the quality control of butanedisulfonic acid ademetionine or related preparations. In the application, the detection of ademetionine and related impurities is not affected by excipients, and has the advantages of high efficiency, convenience, good reproducibility, strong specificity, high accuracy and high sensitivity, and can realize the quality control of ademetionine samples.

The invention discloses a preparation method of an antibacterial epoxy resin coating, and relates to the technical field of coatings. According to the invention, the 5-phenylpent-2, 4-dienoic acid is esterified and combined with the side chain of the epoxy resin, so that the hardness and heat resistance of the cured coating are improved; then addition reaction with dimethyl diallyl ammonium chloride, 2-acrylamide phenylboronic acid and the modified antibacterial agent is performed to form a three-dimensional network structure, so that the mechanical strength of the coating is remarkably enhanced, the antibacterial, high-adhesion and anti-stripping effects are achieved, a phenylboronic acid structure is assisted, a protective film is formed on the surface of an object, the object is effectively isolated from the external environment, and the service life of the coating is prolonged. Oxidation and corrosion of the object surface are prevented, and the corrosion-resistant effect is achieved. According to the modified antibacterial agent, S-adenosine-L-homocysteine and silver nitrate are subjected to a coordination reaction, and the antibacterial effect is achieved; then, the silicon dioxide is combined with vinyl trichlorosilane, silane groups are introduced, after curing, the mechanical strength and water resistance of the coating are remarkably enhanced, corrosive liquid is prevented from entering the coating, and the corrosion-resistant effect is indirectly improved.

A method for identifying pre-disposition to cognitive decline in a subject, the method comprising determining levels of: (a) omega-3 fatty acids, and vitamin D or a metabolite thereof; (b) omega-3 fatty acids, and homocysteine; (c) vitamin D or a metabolite thereof, and homocysteine; or (d) omega-3 fatty acids, vitamin D or a metabolite thereof, and homocysteine, independently in one or more samples obtained from the subject.

The invention discloses a gastrointestinal tumor patient postoperative weakness risk correlation analysis method and system fused with a co-disease map, and belongs to the field of medical care informatics, and the method comprises the following steps: obtaining clinical pathological characteristics, co-disease assessment, serum biomarkers and social and psychological assessment scale data of gastrointestinal tumor patients; obtaining statistical effect values of homocysteine and health attainment, and executing isomorphic mapping; identifying a chained intermediary conduction path and generating a coupling strength factor, and performing weighted correction on the initialized weight matrix; inputting the multi-source data as a risk source excitation signal into the atlas, executing correction calculation, and outputting a risk prediction score; a maximum weight subgraph search is performed to generate risk attribution analysis data. According to the method, the multi-dimensional heterogeneous association map is constructed to fuse the multi-source data of the patient and the medical priori knowledge, and the risk conduction is quantified through the path topology analysis and the matrix correction operation, so that the accurate prediction of the postoperative weakness risk and the interpretable attribution of the key pathogenic path can be realized.

The invention discloses a strain of Weizmania coagulans AUSA001 which is separated from human milk and an application of the strain of Weizmania coagulans AUSA001. The bacillus coagulans AUSA001 provided by the invention is preserved in Guangdong Microbial Culture Collection Center, the preservation date is March 15, 2024, and the preservation number is GDMCC.No.64416. The bacillus coagulans AUSA001 provided by the invention has the advantages that the bacillus coagulans AUSA001 is preserved in the Guangdong Microbial Culture Collection Center; the Witzia ciliaris AUSA001 provided by the invention can be used for preparing a preparation for reducing homocysteine and preventing and treating cerebral apoplexy.

The invention provides a synthesis method of S-allyl sulfydryl cysteine (SAMC), S-allyl sulfydryl-glutathione (GSSA) and S-allyl sulfydryl-homocysteine (SAMC). The invention further provides a preparation method of the S-allyl sulfydryl-glutathione (GSSA) and the S-allyl sulfydryl-homocysteine (SAMC). Specifically, allicin generated by reaction of alliin and allinase is taken as a substrate and reacts with cysteine (Cys), glutathione (GSH) and homocysteine (Hcy) to generate SAMC, GSSA and SAMHC. According to the method, high-purity SAMC, high-purity GSSA and high-purity SAMHC can be obtained, and the yield is high.

The invention belongs to the technical field of electrochemical analysis methods, and particularly relates to a copper nanowire-coated Py-PB-COF compound modified electrode, a preparation method of the copper nanowire-coated Py-PB-COF compound modified electrode and a detection method of homocysteine. The preparation method comprises the following steps: uniformly mixing and grinding a copper nanowire dispersion liquid and a Py-PB-COF material to obtain a copper nanowire-coated Py-PB-COF composite material, mixing and grinding the copper nanowire-coated Py-PB-COF composite material, nano graphite powder and paraffin oil, and self-assembling to prepare the compound modified electrode, and the method is simple and easy to operate. When the prepared compound modified electrode is applied to detection of homocysteine, the linear range is wider, the sensitivity and the selectivity are good, and the reproducibility and the stability are good.

The invention belongs to the technical field of synthetic biology, and particularly relates to a genetically engineered bacterium for high yield of L-methionine as well as a construction method and application of the genetically engineered bacterium. By strengthening the hemC gene, the utilization capacity of escherichia coli on a sulfur source is improved; by strengthening the malY gene and the multi-copy metB gene, the capability of converting cysteine into homocysteine in the escherichia coli is improved; by knocking out an mnaT gene and introducing metA to a genome to resist feedback, the degradation of L-methionine is reduced, and meanwhile, the supply of a precursor O-succinyl homoserine is increased; and finally, a pAmZK plasmid is introduced to obtain the escherichia coli genetically engineered bacterium strain with high yield of L-methionine, and the yield is increased from 3.21 g / L to 3.67 g / L. And fed-batch fermentation is carried out in a 5L tank in a pH feedback feeding manner, the yield reaches 43.55 g / L within 64 hours, and a research foundation is laid for production of L-methionine by a microbiological method.

The invention discloses a cardiovascular event risk index calculation method, and relates to the technical field of medical information, and the method comprises the following steps: determining whether a to-be-evaluated patient meets an application condition or not according to a preset inclusion standard; for a patient meeting the standard, collecting a peripheral blood sample of the patient, carrying out centrifugal treatment, and separating to obtain a serum or plasma sample; s2, quantitatively measuring four serological indexes, namely lipoprotein-associated phospholipase A2, high-sensitivity C-reactive protein, homocysteine and serum amyloid protein A, of the serum or plasma sample obtained in the step S1 by using an authenticated laboratory detection method; s2, calculating a cardiovascular risk index by using a cardiovascular risk calculation formula based on the four index values measured in the step S2; s3, according to the cardiovascular risk index value calculated in the step S3, dividing the cardiovascular event risk of the patient into three levels of low risk, medium risk and high risk; and S4, generating a structured risk assessment report according to the risk grading result obtained in the step S4, and outputting the structured risk assessment report.

The invention provides a micromolecule derived from allicin in vivo and application of the micromolecule. Specifically, the small molecule derived from allicin in vivo comprises S-allyl sulfydryl-homocysteine (SAMC) generated by reaction with homocysteine (HCY), S-allyl sulfydryl cysteine (SAMC) generated by reaction with cysteine (Cys), and S-allyl sulfydryl-glutathione (GSSA) generated by reaction with glutathione (GSH). The micromolecule can be used for detecting the content of homocysteine, cysteine and / or glutathione in the body of a subject and / or detecting whether allicin exists in the body of the subject.

The invention discloses a kit and a system for auxiliary diagnosis of children autism, and relates to the technical field of medical diagnosis. The kit and the system disclosed by the invention take arachidonic acid, tetracosanoic acid, S-adenosylmethionine, oxidized glutathione, glutathione, S-adenosylhomocysteine, choline, glycine, L-serine, cystathionine, L-cysteine, taurine, folic acid or vitamin B12 as biomarkers, and are used for auxiliary diagnosis of children autism; the specificity and the sensitivity are relatively high.

The invention discloses application of S-adenosylhomocysteine in preparation of a medicine for treating neuromuscular diseases, and belongs to the technical field of medicines. The invention discloses a novel pharmaceutical application of S-adenosine homocysteine in preparation of drugs for treating neuromuscular diseases for the first time. Mechanism research finds that S-adenosylhomocysteine can enhance the ability of a Trmt61a / 6 compound to bind unmethylated tRNA and inhibit abnormal interaction between CMT2 pathogenic mutant protein and stress particle core protein, so that the ability of motor neurons to resist external stress is enhanced, and the S-adenosylhomocysteine has the effects of improving peripheral nerve and muscle lesions of neuromuscular diseases, improving the immunity of the motor neurons and improving the immunity of the motor neurons. And the potential of motor nerve dysfunction. The invention provides a direction for new clinical application of the S-adenosyl homocysteine. The invention provides possibility for preparing novel medicines for treating neuromuscular diseases.

The invention belongs to the technical field of detection of biological mercaptan, and provides a mode recognition method of homocysteine, L-cysteine and glutathione, which comprises the following steps: preparing two different sequences of GCCCCCCCG and ssDNA (single-wire carbon nanotubes) coated (6, 5)-SWCNT of GCCCG to construct an array sensor, and combining near infrared fluorescence spectrum determination and linear discriminant analysis to recognize the mode of homocysteine, L-cysteine and glutathione. The analysis and identification of homocysteine, L-cysteine and glutathione are realized. The method is good in selectivity, rapid in detection, low in cost and visual in data, and does not need a complex sample pretreatment process.

The application discloses a series of homocysteine responsive scavenging systems, which are obtained by Schiff base reaction of a material containing cystamine and / or selenocystamine and a polyaldehyde monomer. The series of homocysteine responsive scavenging systems have high stability, can be uniformly and stably dispersed in an aqueous solution, and can specifically scavenge homocysteine which is highly expressed in patients with depression, so that rapid and stable blood risk factor scavenging is realized. The application exhibits rapid, efficient and specific homocysteine scavenging effect, while maintaining excellent biological safety, which is crucial for guaranteeing the safety of patients in the treatment process. Therefore, the COFs-based homocysteine responsive scavenging system is expected to become a new and efficient treatment method for treating depression and other homocysteine-related diseases, and bring revolutionary changes to the research and clinical practice in the related field.

The invention relates to the technical field of model construction, in particular to a zebra fish homocysteine model construction method. The construction method of the zebra fish homocysteine model provided by the invention comprises the following steps: applying a reagent containing DL-homocysteine into a culture solution of zebra fish, and culturing to obtain the zebra fish homocysteine model. The zebra fish homocysteine model constructed by the zebra fish homocysteine model construction method provided by the invention is short in time, short in period of screening drugs for treating cardiovascular or nervous system diseases, and high in efficiency.

The invention relates to the field of biological medicines, in particular to application of an MAT2A inhibitor in preparation of a medicine for treating hyperhomocysteinemia. The MAT2A inhibitor plays an important role in homocysteine regeneration in a metabolism remethylation pathway of homocysteine, by using the MAT2A inhibitor, the homocysteine regeneration can be blocked, the content of the homocysteine is effectively reduced, and methionine serving as essential amino acid can be metabolized through other pathways, so that the homocysteine metabolism remethylation pathway of the homocysteine can be promoted, and the homocysteine metabolism remethylation pathway of the homocysteine can be promoted. No toxic or side effect is caused on a using object.

The invention belongs to the technical field of biology, and particularly relates to application of a reagent for inhibiting an FOXO3a gene in preparation of a medicine for treating homocysteine induced metabolism-related fatty liver disease, and the base sequence of the FOXO3a gene is shown as SEQ ID NO.1. According to the application, Western blot is used for analyzing high methionine diet induced Cbs < + / -> mouse liver tissues and Hcy treatment group liver cells, it is clear that the expression of FOXO3a in the Hcy induced metabolism-related fatty liver diseases is remarkably up-regulated, and it is clear that FOXO3a can influence the occurrence of the metabolism-related fatty liver diseases through mediating mitochondrial damage.

The invention belongs to the field of biotechnology and medical technology, and particularly relates to application of a JunB expression inhibitor in preparation of a medicine for treating atherosclerosis. According to the application disclosed by the invention, homocysteine is found to induce atherosclerosis by promoting JunB expression and reducing the autophagy level of macrophages; after the JunB plasmid is transfected and knocked down to the macrophages, the number of the autophagosomes and the number of the autophagosomes and the number of the autophagosomes are increased; and after the overexpressed JunB plasmid is transfected to the macrophage, the number of the autophagosome and the number of the autolysosome are reduced, which indicates that the expression inhibitor of the JunB can be effectively used for preparing the medicine for treating the atherosclerosis, and provides possibility for developing a new medicine for treating the atherosclerosis.

The invention provides application of a ratiometric fluorescent probe for high-selectivity detection of colorectal cancer marker homocysteine for tumor diagnosis, and relates to the field of analytical chemistry. The structural formula of the ratiometric fluorescent probe is shown in the specification. The ratio fluorescent probe adopts a double binding site strategy, nitryl is introduced into a coumarin fluorophore to realize specific detection of homocysteine, and meanwhile, a chromophore with strong green fluorescence is connected through piperazine, so that ratio detection of homocysteine is realized. Potential interference of background fluorescence can be avoided through the ratio of red fluorescence to green fluorescence, so that the sensitivity and precision of homocysteine detection are improved. In addition, the probe has the advantages of good selectivity, good biocompatibility and the like when being used for detecting homocysteine, and has a huge application prospect in the technical fields of analytical chemistry, life science, biomedical treatment and the like.

The application provides a copper plating solution and a stabilizer, which comprises copper sulfate pentahydrate, a complexing agent, formaldehyde, ethanolamine, 2,2-bipyridine, S-adenosyl-L-homocysteine, S-carboxymethyl-L-cysteine and thiomalic acid. The S-adenosyl-L-homocysteine and the S-carboxymethyl-L-cysteine can improve the tolerance of the plating solution to leached palladium. After a certain amount of thiomalic acid is added, the three components have a good synergistic effect. The plating solution maintains high activity and further improves the tolerance to leached palladium, which is beneficial to improve the service life of the plating solution and prolong the decomposition time of the plating solution. Even under high leached palladium concentration, the thick copper solution does not affect the plating activity and the uniform plating capacity of the chemical copper plating solution. Under normal production load, a deposition rate of 1.0 um / 20 min can be obtained. The solution has good stability. Under extreme conditions of high leached palladium, the decomposition time of the plating solution is prolonged by 2-6 hours.

Methodology was developed for transformation of methionine residues into homocysteine derivatives. Methionine residues can undergo alkylation reactions at low pH to yield sulfonium ions, which can then be selectively demethylated to give alkyl homocysteine residues. This process tolerates many functional groups.

The invention relates to the technical field of measurement for diagnosis, and discloses a homocysteine detection system for noninvasive body fluid sampling, which comprises a noninvasive body fluid sampling module integrated on a flexible substrate, a photoelectrochemical detection module and a signal processing and control unit. The noninvasive body fluid sampling module applies micro current through an electroosmosis electrode to execute reverse ion electroosmosis, and drives subcutaneous interstitial fluid to directionally migrate and be enriched in the hydrogel layer; the photoelectrochemical detection module is in fluid communication with the hydrogel layer, and the surface of a photoelectrochemical electrode assembly of the photoelectrochemical detection module is provided with a double-monatomic imprinting recognition layer. The recognition layer constructs a recognition hole with highly complementary spatial configuration and electronic structure based on the double-site synergistic coordination effect of sulfydryl and Au site and amino and Zn site of homocysteine molecules. Under the condition that invasive blood sampling is not needed, interference of molecules with similar structures in a complex body fluid matrix is overcome, and high-selectivity, high-sensitivity and continuous dynamic monitoring of homocysteine is achieved.

The invention provides a sulfur-containing amino acid solution type or freeze-drying type calibration product and a quality control product and a preparation method thereof. The sulfur-containing amino acid is one or two or three of homocysteine, cysteine or methionine; the preparation method of the freeze-dried calibration product and the quality control product comprises the following steps: preparing the matrix containing the sulfur-containing amino acid into freeze-dried powder through a vacuum freeze-drying technology, so as to obtain the freeze-dried calibration product and the quality control product containing the sulfur-containing amino acid. The preparation method of the solution type calibrator and the quality control product comprises the step of adding the combined stabilizer into the sulfur-containing amino acid solution to obtain the sulfur-containing amino acid solution type calibrator and the quality control product. The sulfur-containing amino acid freeze-drying type or solution type calibrator and quality control product prepared by the preparation method disclosed by the invention have good uniformity and can be stably stored for a long time at the temperature of 2-8 DEG C.

Provided in the present application is a ratiometric fluorescent probe for detecting a colorectal cancer marker homocysteine with high selectivity and a preparation method thereof and relates to the field of analytical chemistry. The ratiometric fluorescent probe has structural formula of:Dual-site binding strategy is adopted in the ratiometric fluorescent probe and the nitro group is introduced into the coumarin fluorophore to achieve specific detection of homocysteine, at the same time, ratiometric detection of homocysteine is achieved by connecting a chromophore having strong green fluorescence through piperazine. Potential interference of background fluorescence can be avoided by the ratio of red fluorescence to green fluorescence, thereby enhancing the sensitivity and accuracy of homocysteine detection. In addition, the probe has the advantages of good selectivity and biocompatibility in detecting homocysteine, and has great application prospects in technical fields such as analytical chemistry, life sciences and biomedicine.

The present application relates to the technical field of biological medicine, and particularly relates to a histidine methyltransferase METTL9 inhibitor and a screening method, a reaction system and application thereof.The screening method comprises the following steps: S1, constructing a histidine methyltransferase METTL9 reaction system; S2, dividing the reaction system into a test system and a control system, adding S-adenosylhomocysteine hydrolase and a fluorescent dye capable of undergoing an addition reaction with homocysteine and causing a change in fluorescence signal; S3, adding a candidate compound to the test system, and not adding the candidate compound to the control system, and detecting the fluorescence signals of the test system and the control system; and S4, determining whether the candidate compound is a METTL9 inhibitor based on the fluorescence signals.The screening method couples the enzymatic reaction with the fluorescence signal transduction, has the advantages of high sensitivity, simple operation, good safety, low cost and being particularly suitable for automatic high-throughput screening.