5 results about "Homocysteine" patented technology

The present application relates to the technical field of biological medicine, and particularly relates to a histidine methyltransferase METTL9 inhibitor and a screening method, a reaction system and application thereof.The screening method comprises the following steps: S1, constructing a histidine methyltransferase METTL9 reaction system; S2, dividing the reaction system into a test system and a control system, adding S-adenosylhomocysteine hydrolase and a fluorescent dye capable of undergoing an addition reaction with homocysteine and causing a change in fluorescence signal; S3, adding a candidate compound to the test system, and not adding the candidate compound to the control system, and detecting the fluorescence signals of the test system and the control system; and S4, determining whether the candidate compound is a METTL9 inhibitor based on the fluorescence signals.The screening method couples the enzymatic reaction with the fluorescence signal transduction, has the advantages of high sensitivity, simple operation, good safety, low cost and being particularly suitable for automatic high-throughput screening.

The application discloses a preparation method and application of S-adenosyl-methyl cysteine, and the preparation method comprises the following reaction: S-adenosyl-L-cysteine is methylated by using a first methyltransferase or a first methyltransferase mutant and a methyl donor to obtain S-adenosyl-methyl cysteine; or S-adenosyl-L-homocysteine is methylated by using a methyltransferase or a first methyltransferase mutant and a methyl donor to obtain S-adenosyl-methionine. The method has the characteristics of low cost and simple operation, and enables S-adenosyl-methyl cysteine to replace expensive S-adenosyl methionine (SAM) as a coenzyme and a methyl donor in a methylation reaction, and is applied to methylation modification of natural products based on a coenzyme regeneration system.

The present application relates to the technical field of metabolite detection, and particularly relates to a method and a kit for detecting homocysteine and metabolically related substances. The present application provides a method for detecting homocysteine and metabolically related substances, comprising the following steps: mixing the to-be-detected sample, S1-S6 standard, high-value quality control and low-value quality control with reagent D respectively, standing, then mixing with a reducing agent for reaction, and then mixing with a formic acid acetonitrile solution to obtain a mixture; mixing the mixture with water, centrifuging, taking the supernatant for liquid chromatography-tandem mass spectrometry detection. The method can detect three types of compounds including homocysteine, cysteine and methionine indicators at one time, and comprehensively reflects the reasons for the increase of homocysteine.

The application discloses application of a piRNA-145836 preparation in preparation of a medicine for preventing and treating type 2 diabetes induced by high homocysteine, and belongs to the technical field of biological medicine and molecular biology. The nucleotide sequence of the piRNA-145836 is shown as SEQ ID No. 1. The piRNA-145836 can play a key role in type 2 diabetes induced by high homocysteine, and can regulate a mechanism, promote recovery of pancreatic function, restore insulin secretion capacity, reduce abnormal activation of copper death-related proteins, reduce accumulation of copper ions in islet beta cells, and improve sugar metabolism disorder induced by high homocysteine. The application solves the technical problems of lack of cognition of the role of piRNA in copper ion homeostasis and beta cell fate determination, ambiguity of the role target of resveratrol, and unknown expression regulation mechanism of key copper transport proteins in a high homocysteine environment in the prior art.