375 results about "Metabolite" patented technology

The present invention relates to the use of a combination of a saccharide and glycerol for skin protection against undesirable bacteria. The present invention is particularly useful in the formulation of compositions that act as prebiotics for commensal skin bacteria such as S. epidermidis to produce metabolites such as lactic acid, which, through the present invention, has been shown to inhibit the growth of undesirable bacteria such as E. coli and S. aureus, among others. The present invention is especially useful because it provides both short-term (i.e., rapid) and long-term (or sustained) protection.

This invention belongs to the technical field of environmental health risk assessment and in vitro toxicology evaluation, specifically relating to an in vitro evaluation method for the human fate assessment of oral exposure to chlorinated antibacterial agents. This invention establishes an integrated evaluation process combining simulated digestion, liver metabolism, metabolite screening, and toxicity prediction. It can systematically characterize the in vitro fate process after oral exposure to chlorinated antibacterial agents and introduces a computational toxicology prediction platform to predict the toxicity endpoints of metabolites, identifying the risk of metabolic activation or enhanced toxicity, thus compensating for the shortcomings of focusing only on the parent compound.

The application discloses an Aeromonas media strain capable of degrading PAEs and an application thereof, and belongs to the technical field of microorganisms. The strain is Aeromonas media HJ02, and the preservation number is GDMCC NO: 66828. The strain has obvious advantages in environmental adaptability and PAEs pollutant degradation activity, can efficiently degrade various PAEs and intermediate metabolites, and maintains good growth kinetic characteristics in a 0-120 h culture period. When the initial concentration of diisooctyl phthalate (DEHP) is 300 mg / L, the strain can significantly reduce the residual content of DEHP in the culture system after culture, and the 24 h degradation rate reaches 91.73 %. The application has the advantages of simple operation, safety and economy, provides efficient and stable microbial resources for PAEs pollution remediation, and has important practical value.

The application discloses a seminal plasma metabolic biomarker combination for male infertility spermatogenesis obstacle typing diagnosis, and relates to the field of biological science and technology.The application records the metabolic fingerprints of 475 seminal plasma samples by means of nanoparticle-enhanced laser desorption / ionization mass spectrometry, and constructs a seminal plasma metabolic fingerprint database related to spermatogenesis obstacle (SPGF). Then, through machine learning and statistical analysis, six metabolic biomarkers for SPGF typing diagnosis are screened and identified in the seminal plasma, and the six metabolic biomarkers can realize accurate typing of SPGF (AUC=0.814). The application has the advantages of simple sample pretreatment, fast analysis speed and low cost by means of recording the metabolic fingerprints of seminal plasma by means of nanoparticle-enhanced laser desorption / ionization mass spectrometry. Through the synthesized iron oxide nanoparticles as a matrix, small molecule metabolites in the seminal plasma can be efficiently captured and ionized, and complex pretreatment steps such as deproteinization and chromatographic separation are not needed, so that super-fast analysis, super-high sensitivity, extremely low sample consumption and excellent repeatability detection are realized.

This application relates to the field of tumor treatment technology, specifically to a drug combination and evaluation method for improving the sensitivity of MSS CRC to anti-PD-1 / PD-L1 therapy. The drug combination comprises vancomycin and an immune checkpoint inhibitor. Vancomycin is used to remodel the gut microbiota and reduce L-asparagine levels; the immune checkpoint inhibitor is used to activate CD8. + T-cell anti-tumor response. By combining vancomycin with immune checkpoint inhibitors, the antigen-presenting capacity of dendritic cells is enhanced, thereby increasing the sensitivity of MSS CRC to anti-PD-1 or anti-PD-L1 immunotherapy. This application focuses on the upstream initiation link of gut microbiota-metabolites-dendritic cells-immune activation, relieving the inhibition of dendritic cell antigen presentation by L-asparagine and enhancing CD8+. + T cell activation and tumor immune response enhance the sensitivity of MSS-type colorectal cancer to anti-PD-1 / PD-L1 therapy.

This invention relates to a method for efficiently spraying soil conditioner to treat saline-alkali land using composite microorganisms, belonging to the field of soil improvement technology. The method involves screening salt-tolerant functional microbial strains to prepare a composite microbial agent, which is then mixed with carrier materials such as diatomaceous earth, soybean meal, yeast metabolites, and well-rotted farmyard manure in a specific ratio to form a conditioner for application. The conditioner is applied using a two-stage spraying method, combined with deep tillage pretreatment and controlled temperature and humidity conditions, significantly improving microbial activity and soil condition uniformity. Experimental data show that after applying this method, the soil pH value decreased to 7.3-7.5, the salt content decreased to 0.1%-0.2%, and the organic matter content increased to 18-22 g / kg, achieving efficient ecological restoration and sustainable utilization of saline-alkali land.

The application discloses application of a coumarin compound or a composition thereof in inhibition of a plant Huanglongbing pathogen, prevention of plant Huanglongbing, improvement of plant resistance to Huanglongbing, inhibition of a plant pathogenic bacterium and preparation of a product for preventing and treating plant Huanglongbing, improving plant Huanglongbing resistance, inhibiting a plant Huanglongbing pathogen and inhibiting a plant pathogenic bacterium. The coumarin compound is isofraxidiformin or ostruthin. By applying the coumarin compound or the composition thereof to a receptor plant or a growth medium thereof, the content of the coumarin compound or the composition thereof in the receptor plant is increased, and then the citrus Huanglongbing pathogen in the plant body is significantly reduced C Las quantity. Under field application conditions, the coumarin compound or the composition thereof also shows good prevention and treatment potential. In addition, the coumarin compound shows broad-spectrum bacteriostatic activity on a plurality of other plant pathogenic bacteria, and shows good plant antibacterial prevention and treatment potential.

Disclosed herein is deuterated buprenorphine as a protective agent for fetal subjects against full-agonist opioid exposure. Use of deuterated buprenorphine prevents or reduces exposure of the fetus to opioids used by the mother. Use of deuterated buprenorphine may prevent or reduces exposure of the fetus to harmful metabolites of buprenorphine.

The application relates to a benzene ring-containing compound with analgesic efficacy, a preparation method and use thereof, and relates to the field of medicinal chemistry. The compound is a compound shown in formula I, or a stereoisomer thereof, or a pharmaceutically acceptable salt thereof, or a solvate thereof, or a crystal form thereof, or a prodrug thereof, or a metabolite thereof, or a deuterium derivative thereof. The analgesic effect of the compound is excellent, the compound is safe to use, has small toxic and side effects, and does not produce dependence during use. Therefore, the compound has a wide application prospect in the preparation of analgesic drugs, and provides a new selection for preparing drugs with analgesic action in the clinic.

The application discloses a metabolite and application thereof in diagnosing SLE, and the metabolite is Benzoyl-DL-arginine-naphthylamid. The application firstly finds that the metabolite as a biomarker presents significant difference between SLE patients and healthy people, and has a higher AUC value, which indicates that the metabolite marker has higher diagnostic value in SLE, and has wide application prospect in clinic.

This application discloses a purino[1,2-a]carbazolin derivative, its preparation method, and its application. Specifically, it discloses a compound having the structure shown in Formula I, or its stereoisomers, solvates, metabolites, pharmaceutically acceptable salts, cocrystals, or prodrugs: ; wherein R1 and R2 are each independently selected from hydrogen, hydroxyl, carboxyl, substituted or unsubstituted C1-C6 alkyl, -CONH-R3, and -COO-R4; wherein R3 is a substituted or unsubstituted C1-C5 alkyl, and the substituent can be any of cyano, halogen, alkylC1-C5 alkoxy, C1-C5 alkylsulfonyl, di(C1-C5 alkyl)amino, nitrogen-containing heterocyclic, aryl, and C1-C5 heteroaryl; R4 is selected from any of substituted or unsubstituted aryl C1-C5 alkyl and nitrogen-containing saturated heterocyclic C1-C5 alkyl. This compound can be used as a TDP1 inhibitor for antitumor therapy.

This invention discloses a method for producing amino acid-containing water-soluble fertilizer using sugarcane molasses and organic oilseed cake. The method involves first establishing an equation relating the composting time of organic oilseed cake to influencing factors, then performing material mixing, enzymatic hydrolysis, centrifugation, filtration, enzyme inactivation, preservation, and adjustment of the effective component content to obtain the amino acid-containing water-soluble fertilizer of this invention. This invention can rapidly compost organic oilseed cake within 12 hours, resulting in a stable fertilizer product with a storage period of ≥3 months. It will not undergo further enzymatic hydrolysis to produce odors or secondary metabolites, and its solubility is ≥95%, which is beneficial for the promotion of fertigation technology. The fertilizer has a high amino acid content, and the nutrient content can be adjusted as needed, significantly improving crop yield and quality after application.

This invention relates to a resin-based nano-iron oxide-enhanced microbial preparation, a method for preparing the same, and its applications. In this invention, after mixing resin-based nano-iron oxide, the microbial preparation, and food waste, at room temperature, the resin-based nano-iron oxide enhances the decomposition of food waste by the microbial preparation through multiple actions, including promoting microbial growth, inducing high activity of functional proteases, and accelerating electron transport processes. The alternating aerobic and anaerobic environments allow the microbial preparation to exert its full effect, resulting in high biochemical activity of metabolites. A reaction under room temperature conditions of 3 to 5 hours can achieve a food waste reduction rate of 80% to 99%. This invention is technically simple, consumes little energy, does not cause secondary pollution, and effectively achieves the goals of food waste reduction, resource recovery, and detoxification.

This invention discloses a method for inducing and culturing burdock stem cells and stem cells, belonging to the field of plant biotechnology. Using burdock roots as explants, the cambium is determined by staining, and the cambium is obtained using enzymatic digestion. The cambium is then inoculated into a specific ratio of induction solid medium and cultured in the dark to efficiently induce primary stem cells. These primary stem cells are then transferred to a proliferation solid medium for subculture and selection to obtain and maintain a vigorous, soft stem cell line. The solid stem cell line is further transferred to a liquid medium for shaking culture to establish a suspension stem cell line. This invention provides high induction efficiency, stable stem cell characteristics, and good reproducibility, offering a high-quality starting material and technical platform for in vitro preservation of burdock germplasm, large-scale production of secondary metabolites, and genetic transformation.

The application discloses a kind of genetic engineering escherichia coli plasmid expression culture medium and fermentation process, including basic medium and feed medium;The basic medium includes glycerol, yeast hydrolysate, yeast peptone, L-glutamic acid, L-valine, L-isoleucine, arginine, tyrosine, lysine, proline, glycine, ammonium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, citric acid, magnesium sulfate, ferrous sulfate, zinc sulfate, calcium chloride, manganese chloride, cobalt chloride, copper chloride, sodium ethylenediaminetetraacetate;Feed medium includes glycerol, citric acid, magnesium sulfate, ferrous sulfate, zinc sulfate, calcium chloride, manganese chloride, cobalt chloride, copper chloride, sodium ethylenediaminetetraacetate.The application inhibits the accumulation of harmful metabolites, and creates the optimal intracellular environment for stable replication of plasmid.In industrial fermentation scale, the amount of plasmid harvested per liter of fermentation broth is more than 1 gram, and the supercoiling ratio of plasmid DNA is stably maintained at more than 90%, with minimal batch-to-batch variation.

The present application belongs to the field of biotechnology, and particularly relates to a preparation method and application of five compounds (1-5) extracted from liquid fermentation of Chaetomium sp. Specifically, the present application discloses five compounds, wherein compound 1 is a new compound. The Chaetomium sp. metabolite has good antibacterial activity on three tested pathogenic bacteria (Pseudomonas syringae pv. actinidiae, Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola), and has important significance for development of a new type of microbial source antibacterial agent.

This invention discloses a method for the synergistic metabolic analysis of multiple components of traditional Chinese medicine (TCM) integrated with the gut microbiota. After initializing the microbiota ecosystem, a solution of multi-component TCM extracts is continuously injected into a microfluidic chip. Stable isotope-labeled precursors are pulsed into a specific simulation region to perform spatial isotope encoding. A standing wave acoustic field is applied at the sampling node of the microfluidic chip, and multiphase fluid spatial splitting is performed using the difference in the primary acoustic radiation force experienced by the particles to derive cell-free TCM metabolites. The cell-free TCM metabolites are separated by chromatography and gas phase drift separation. A four-dimensional metabolic feature array is constructed, and the absolute value of the mass shift is calculated for spatial inverse decoding. Closed-loop feedback is triggered based on the rate of change of target metabolite ion abundance, increasing the amplitude of the standing wave acoustic field pressure to confine intestinal bacteria and performing in-situ enrichment of synergistic metabolic intermediates. This invention realizes the spatial metabolic trajectory tracing and network reconstruction of multiple components of TCM in a complex gut microbiota ecosystem.

This invention discloses an antibody targeting a specific protein of *Lactobacillus* and its applications. The monoclonal antibody of this invention is any one of monoclonal antibody 1B3, monoclonal antibody 3B5, monoclonal antibody 2E5, and monoclonal antibody 3D8, which respectively recognize proteins Srt A, Cps C, ENO 1, and Fts Z. The magnetic bead antibody conjugate is prepared by conjugating the monoclonal antibody with activated carboxyl magnetic beads. The magnetic bead antibody conjugate of this invention can specifically enrich *Lactobacillus* bacteria with high enrichment efficiency and simple operation. It can be applied to the identification of metabolites of *Lactobacillus* bacteria, as well as their qualitative and quantitative analysis.

This invention discloses a method for screening sulfur-free biomarkers in fresh bamboo shoots and a detection method based on VB1 and its metabolites, belonging to the field of food preservation technology. The method selects sulfur (S), thiol, adenosine, VB1, and its metabolite 4-methyl-5-(beta-hydroxyethyl)thiazole as sulfur-free biomarkers for fresh bamboo shoots. The detection of sulfur-fumigated foods based on VB1 and its metabolites includes: 1. Determination of VB1 content; the VB1 content in sulfur-fumigated bamboo shoots is lower than that in fresh bamboo shoots; 2. Determination of 4-methyl-5-(beta-hydroxyethyl)thiazole content; sulfur-fumigated bamboo shoots contain 4-methyl-5-(beta-hydroxyethyl)thiazole. Through the above methods, this invention can identify whether bamboo shoots have been sulfur-fumigated by determining the content of VB1 and 4-methyl-5-(beta-hydroxyethyl)thiazole in bamboo shoots.

The application discloses a kind of based on carbon-based modified electrode microbial quantity dynamic evaluation method.The method comprises the following steps: preparing carbon-based modified electrode doped with target microorganism;Carbon-based modified electrode is used as working electrode, and reference electrode and counter electrode constitute three-electrode system, and is placed in the medium containing target microorganism to be measured in the electrochemical scanning of target microorganism, and the electrochemical response signal is collected;The electrochemical response signal is processed, and the characteristic electrochemical parameter of target microorganism metabolite is obtained;According to the preset detection time threshold selection model: when detection time is less than or equal to preset duration, linear model is used;When detection time is greater than preset duration, dynamic metabolism model is used;Characteristic electrochemical parameter is substituted into the selected model, and the number of microorganisms in the medium to be measured is calculated.The application can realize accurate quantification, anti-interference detection and rapid dynamic evaluation of the number of microorganisms, and significantly improve detection efficiency and accuracy.

This invention discloses gold nanoparticle-modified metal oxide framework materials, their preparation methods, and applications, relating to the fields of material preparation and detection technology. The preparation method of the gold nanoparticle-modified metal oxide framework material includes: in-situ reduction of chloroauric acid on the surface of ZIF-67 via a reduction method to obtain an Au / ZIF-67 composite material; and calcination of the Au / ZIF-67 composite material using a programmed temperature rise process to obtain the final product. This invention also provides applications of this material in serum metabolite detection and in screening metabolic biomarkers for esophageal cancer diagnosis. The composite material prepared by this invention possesses a unique hollow porous structure and uniformly distributed gold nanoparticles, providing a large specific surface area for enriching metabolites in serum. It also enhances the ultraviolet absorption and photothermal conversion efficiency of the nanocomposite material, while improving the material's conductivity, thereby significantly improving laser desorption / ionization efficiency and achieving high-sensitivity detection.

The application relates to an LC-MS / MS method for detecting neurotransmitter metabolites in a biological sample, comprising the following steps: S1, constructing a mass spectrum database containing neurotransmitter metabolites and isotope internal standards; S2, preparing a mixed standard solution containing neurotransmitter standards and drawing standard curves of each neurotransmitter metabolite standard; S3, extracting a biological sample by a protein precipitation method to obtain a sample solution to be detected; S4, separating the sample solution to be detected by using liquid chromatography, wherein a mobile phase A of the liquid chromatography is an aqueous solution containing ammonium fluoride and formic acid, and a mobile phase B is an organic phase containing formic acid; and S5, performing mass spectrum detection on the sample separated by the liquid chromatography. The method can realize baseline separation of 57 metabolites within 9 minutes.

This invention discloses a method for preparing livestock feed based on the recycling of kitchen waste, relating to the fields of waste resource utilization and feed processing technology. The method includes: transferring raw materials using a closed device; removing inorganic impurities and metals through negative pressure unloading, multi-stage vibration sorting, and magnetic iron removal; adjusting the particle size of the material using a heavy-duty high-speed pulverizer to increase the specific surface area; using high-pressure steam for constant-temperature sterilization to achieve complete harmlessness; inoculating fermentation bacteria after the material has been gradually cooled to a preset range, and carrying out biological fermentation in a controlled environment to produce metabolites; finally, adding corn flour, soybean meal, and wheat bran in proportion for deep mixing. This invention, through the above scheme, achieves complete inactivation of pathogenic bacteria and precise removal of foreign matter, significantly improving the biosafety and digestibility of the recycled feed, realizing a high-value closed-loop recycling of waste, and exhibiting significant environmental and economic benefits.

This invention discloses an alkaloid compound and its uses. Specifically, the invention relates to compounds of formula (I), or stereoisomers, tautomers, nitrides, hydrates, solvates, metabolites, pharmaceutically acceptable salts, or prodrugs of compounds of formula (I), and also to pharmaceutical compositions comprising said compounds and their uses. In particular, this alkaloid compound has a significant inhibitory effect on angiogenesis and can be used as a medicament for the prevention or treatment of tumors, inflammation, ophthalmic diseases, abnormal fibrosis or scar formation, cardiovascular and cerebrovascular diseases, nervous system diseases, infectious diseases, metabolic diseases, and diseases related to abnormal angiogenesis in mammals.

Microscopy imaging that allows for multiple mRNAs, proteins and metabolites to be spatially resolved at a subcellular level provides valuable molecular information which is a crucial factor for understanding tissue heterogeneity as for example within the tumor micro environment. The current invention describes a method (High Density-SUMI-Seq) which combines the use of Spatial Unique Molecular Identifier in situ localization and identification (by in situ sequencing or sequential fluorescence hybridization) of rolonies derived from rolling circle amplification of circular oligonucleotides and in vitro sequencing of target amplified RNA or DNA in combination with SUMI identification at a subcellular level with no optical diffraction limitation in the amount of amplified target information that can be analyzed per cell. Apart from amplified RNA or DNA, the High Density-SUMI-Seq method can also be applied using linear oligonucleotides to spatially resolve proteins and metabolites to provide multiomics results.

The application belongs to the technical field of medicines, and relates to a pyrimidine compound and application thereof in medicines, in particular, application of the pyrimidine compound in preparation of medicines for treating and / or preventing diseases or symptoms caused by a novel coronavirus (SARS-CoV-2). Specifically, the application relates to a compound shown in a general formula (I) or a stereoisomer, a tautomer, a nitroxide, a solvate, a metabolite, a pharmaceutically acceptable salt or a prodrug thereof, a pharmaceutical composition containing the compound, and application of the compound and the pharmaceutical composition in preparation of medicines for preventing and / or treating diseases or symptoms caused by a coronavirus, in particular, application of the compound and the pharmaceutical composition in preparation of medicines for treating and / or preventing diseases or symptoms caused by SARS-CoV-2, wherein each variable in the formula (I) is as defined in the specification.

The present invention relates to a biomarker for predicting an asthma treatment response to a biological agent and a use thereof, and specifically to a method for predicting an asthma treatment effect

The application discloses Lactobacillus reuteri and application thereof in preparation of a medicine for treating diffuse large B-cell lymphoma, and relates to the technical field of biological medicine. The application provides application of Lactobacillus reuteri and / or a metabolite thereof in preparation of a medicine for treating diffuse large B-cell lymphoma, wherein the Lactobacillus reuteri is Lactobacillus reuteri (HG001). Limosilactobacillus reuteri The Lactobacillus reuteri is separated from feces of healthy people and used for preparation of the medicine for treating diffuse large B-cell lymphoma, and it is found that the Lactobacillus reuteri is confirmed as a probiotic strain for enhancing the chemotherapy effect of diffuse large B-cell lymphoma and can improve side effects caused by cyclophosphamide treatment of diffuse large B-cell lymphoma; and the Lactobacillus reuteri and the cyclophosphamide have a synergistic effect in treatment of diffuse large B-cell lymphoma.

The present invention provides compounds represented by formula (I) and their racemates, stereoisomers, tautomers, isotopically labeled compounds, nitrogen oxides, solvates, polymorphs, metabolites, esters, prodrugs, or pharmaceutically acceptable salts thereof. These compounds have good FGFR2 / 3 inhibitory activity and can be used to treat or prevent FGFR2 / 3-mediated disorders and diseases, and can be used to manufacture drugs used for the treatment or prevention of such disorders and diseases. [Formula 1] TIFF2026518289000170.tif53170

This invention discloses an antibody against a specific protein of *E. rectum*, a magnetic bead antibody-conjugate, and their applications. The monoclonal antibody is either monoclonal antibody 4D3F6H7 or monoclonal antibody 5C8E7D6. The amino acid sequences of the heavy chain variable region and light chain variable region of monoclonal antibody 4D3F6H7 are shown in SEQ ID NO: 5 and SEQ ID NO: 6, respectively. The amino acid sequences of the heavy chain variable region and light chain variable region of monoclonal antibody 5C8E7D6 are shown in SEQ ID NO: 7 and SEQ ID NO: 8, respectively. The magnetic bead antibody-conjugate is obtained by conjugating the monoclonal antibody with activated carboxyl magnetic beads. The magnetic bead antibody-conjugate of this invention can specifically enrich *E. rectum* with high enrichment efficiency and simple operation. It can be applied to the identification of *E. rectum* metabolites, as well as their qualitative and quantitative analysis.