1927 results about "Dopamine" patented technology

The invention provides temperature and pH responsive hydrogel entrapped with chlorogenic acid lipidosome as well as a preparation method and application of the temperature and pH responsive hydrogel, and relates to the technical field of biology. The temperature and pH responsive hydrogel entrapping chlorogenic acid lipidosome contains chlorogenic acid lipidosome, phenylboronic acid grafted modified gelatin and dopamine grafted oxidized hyaluronic acid, the concentration of the phenylboronic acid grafted modified gelatin is 3-10 wt%, the concentration of the dopamine grafted oxidized hyaluronic acid is 3-10 wt%, and the concentration of chlorogenic acid is 1-20 [mu] M. The temperature and pH responsive hydrogel can effectively promote wound healing independently or in combination with blue light.

The invention belongs to the technical field of biomedical materials, and particularly relates to adipose-derived mesenchymal stem cell loaded conductive hydrogel and a preparation method and application thereof, the preparation method comprises the following steps: step S1, after hyaluronic acid is subjected to oxidation treatment, dopamine is grafted to oxidized hyaluronic acid, and oxidized hyaluronic acid-dopamine is obtained; step S2, carrying out amination treatment on gelatin to obtain aminated gelatin; s3, mixing the oxidized hyaluronic acid-dopamine with the aminated gelatin, and carrying out Schiff base crosslinking, so as to obtain hydrogel; and step S4, loading the adipose tissue-derived stromal cells by using the hydrogel. Dopamine is grafted on an oxidized hyaluronic acid macromolecular chain through amidation reaction, the hydrophilicity and the electrical property of the macromolecular chain are improved, a catechol structure is provided to provide possibility for iron ion coordination, the tissue adhesion, the injectability, the mechanical property and the electrical conductivity of the hydrogel are improved, and the hydrogel can be applied to the field of biomedical materials. After being applied to a spinal cord injury rat model, the functional recovery can be promoted.

The invention discloses injectable hydrogel with redox activity and electroactivity as well as a preparation method and application of the injectable hydrogel, and belongs to the technical field of medical materials. The preparation method comprises the following steps: reacting methacrylic anhydride and polysaccharide molecules in a solvent to prepare a double-bond functional polymer; the preparation method comprises the following steps: firstly, pre-polymerizing dopamine in a solvent, and then adding MOF (Metal Organic Framework) to carry out polymerization reaction, so as to prepare dopamine modified MOF; reacting the dopamine modified MOF, EDOT and ferric salt in a solvent, and collecting the precipitate to prepare a nano filler; the preparation method comprises the following steps: jointly dispersing the double-bond functional polymer, active polypeptide, a photoinitiator and nano filler into a solvent, and carrying out photocuring cross-linking. The preparation condition is mild, gel forming is rapid, the method is simple, the prepared hydrogel has injectable performance and conductive performance, catalytic activity of natural enzyme can be simulated, active oxygen removal can be achieved in the early stage of myocardial ischemia, electrical conduction of infarction parts can be enhanced, and effective recovery of cardiac functions can be achieved.

The invention relates to a preparation method and application of a Janus structure photo-thermal-piezoelectric hydrogel dressing for infected wound repair, and the preparation method comprises the following steps: respectively dissolving phenylboronic acid grafted methacrylamide gelatin and dopamine grafted hyaluronic acid in deionized water, adding MXene coated CeO2 heterojunction, and ultrasonically dispersing uniformly, so as to obtain the Janus structure photo-thermal-piezoelectric hydrogel dressing for infected wound repair. After mixing, sodium hydroxide is used for adjusting the pH value to alkalescence, and a Janus structure inner layer is formed; respectively dissolving phenylboronic acid grafted gelatin and o-nitrobenzyl alcohol grafted hyaluronic acid in deionized water, adding BaTiO3, ultrasonically dispersing uniformly, and mixing to form a Janus structure outer layer; the Janus structure photo-thermal-piezoelectric hydrogel dressing prepared by the invention has the characteristic of space-time release so as to adapt to the wound repair process, in addition, the Janus structure photo-thermal-piezoelectric hydrogel dressing also has excellent photo-thermal conversion performance, bacteria infecting the wound are killed through photo-thermal heating, physiological electric signals are effectively simulated through the piezoelectric effect, cell behaviors are regulated and controlled, and the Janus structure photo-thermal-piezoelectric hydrogel dressing can be used for wound infection repair.

The invention discloses a high-strength PET composite material and a preparation method thereof, and relates to the technical field of polymer materials. The invention first adds fumed silica during the preparation of graphene aerogel, and the siloxy groups on the surface of the fumed silica combine with the hydroxyl groups of the graphene oxide hydrogel, and the aerogel is mixed after heat treatment, which can improve the strength of the composite material and achieve excellent hydrophobic effect; secondly, dopamine is grafted on the surface of the aerogel, and the amino groups of the dopamine and the silanol groups of the silica undergo dehydration condensation to form a composite swelling layered silicate to prepare a hybrid filler; dopamine improves the dispersibility and compatibility of the filler in PET; when fine cracks appear in the composite material due to aging, the swelling layered silicate and the dopamine rainwater work together to achieve a self-repairing effect, and can also remove free radicals, so that the strength of the composite material is stable and the anti-aging performance is improved. The PET composite material prepared by the invention has high strength and anti-aging effects.

The invention provides a composition for promoting hair regeneration after chemotherapy as well as a preparation method and application thereof, and belongs to the technical field of medicines. The preparation method comprises the following steps: modifying the surface of a gold nanocage with polydopamine, loading the modified gold nanocage on an exosome, complexing magnesium ions and zinc ions to prepare a modified exosome, preparing a nano drug-loaded liposome from curcumin, resveratrol and epigallocatechin gallate, mixing the nano drug-loaded liposome with the modified exosome to prepare a multi-layer liposome, and preparing the multi-layer liposome from the nano drug-loaded liposome. And mixing with a penetration enhancer, and adding into a temperature-sensitive hydrogel system to prepare the composition for promoting hair regeneration after chemotherapy. The composition for promoting hair regeneration after chemotherapy has a good transdermal absorption promoting effect, can slowly release drugs, promotes hair follicles to be converted from a resting stage to a growing stage under the synergistic effect of the drugs, regulates and controls multiple pathways (such as anti-apoptosis, angiogenesis promotion and anti-inflammation) at the same time, is matched with a complex pathological mechanism of hair regeneration after chemotherapy, and can promote hair regeneration after chemotherapy. Wide application prospects are realized.

The invention discloses a preparation method of an antibacterial PEEK material for bone implantation. The preparation method comprises the following steps: carrying out sand blasting treatment on the surface of a PEEK sample; immersing the sandblasted PEEK sample into a mixed solution containing concentrated sulfuric acid and hydrogen peroxide, and carrying out chemical sulfonation treatment; immersing the sulfonated PEEK sample into a dopamine solution, so that dopamine is oxidized and self-polymerized on the surface to form a PDA coating; immersing the PEEK sample with the PDA coating into an antibacterial drug solution, so that the antibacterial drug is loaded on the surface; carrying out heat treatment on the PEEK sample loaded with the antibacterial drug; thoroughly cleaning the sample, removing uncombined antibacterial drug molecules, and drying; through sand blasting treatment and sulfonation treatment, the surface roughness and hydrophilicity of the PEEK material are remarkably improved, and favorable conditions are provided for adhesion of a dopamine coating and an antibacterial agent; due to introduction of the dopamine coating, the biocompatibility and the adhesion of the material are enhanced, and the dopamine coating also serves as an effective carrier of antibacterial drugs, so that the antibacterial function is maximized.

The invention belongs to the field of drug preparation, and particularly relates to a preparation method and application of a drug delivery system based on hollow mesoporous polydopamine nanoparticles. The drug delivery system comprises hollow mesoporous polydopamine nanoparticles, a drug loaded inside and / or outside the hollow mesoporous polydopamine nanoparticles, and pectin wrapping the hollow mesoporous polydopamine nanoparticles, the medicine is a medicine with a nerve protection effect and an acetylcholin esterase inhibitor. The drug delivery system can reduce proinflammatory cytokines, regulate functions of target immune cells, synergistically reduce necrosis of neurons in intestinal tracts and induce neural crest stem cells in the intestinal tracts to be differentiated into neurons.

The invention relates to the technical field of medical dressings, in particular to a perianal drug dressing of a multi-layer flow guide composite fabric and a preparation method and application of the perianal drug dressing of the multi-layer flow guide composite fabric. The perianal drug dressing is of a four-layer structure, a wet tissue adhesion layer is made of polydopamine modified chitosan / oxidized hyaluronic acid hydrogel, and wet-state adhesion is achieved; the drug sustained-release layer is used for dispersing mesoporous silica loaded with ciprofloxacin hydrochloride / defensin and chitosan microspheres loaded with epidermal growth factors / lonicera japonica into a polycaprolactone fiber membrane to achieve synergistic antibacterial and healing promotion; the directional flow guide layer is of a gradient wettability sandwich structure with a bionic micro-channel, and one-way rapid drainage can be achieved; the high-absorption outer layer is carboxymethyl cellulose / sodium polyacrylate composite sponge, liquid is locked efficiently, the wound surface is kept dry and comfortable, the clinical problem in perianal infection wound treatment can be effectively solved, and the treatment effect and the patient comfort are remarkably improved.

The invention discloses a double-crosslinking network hydrogel film for treating oral ulcer, a preparation method and application, and relates to the technical field of biomedical materials. According to the hydrogel film, a dual-network synergistic enhancement structure is constructed through covalent / non-covalent bi-crosslinking, the adhesion to oral mucosa is remarkably enhanced by adopting dopamine (DA) modified hyaluronic acid (HA), and the mechanical property is greatly improved by utilizing a bi-crosslinking network system of disulfide bond / double bond crosslinking (chitosan and CS) and hydrogen bond / catechol self-crosslinking (hyaluronic acid and HA); the antioxidant and anti-inflammatory characteristics of lipoic acid (LA) and the natural antibacterial property of chitosan (CS) are combined to play a synergistic effect, so that the antibacterial and anti-inflammatory effects are remarkably improved. Meanwhile, multifunctional modified materials such as metacrylic acid ester are introduced, so that the functionality and the application range of the hydrogel film are expanded. The preparation technology is optimized, it is ensured that the material is safe and effective, large-scale production is easy, and an efficient and reliable novel material is provided for oral ulcer treatment.

The invention belongs to the technical field of agricultural nanoparticle preparations, and relates to a dopamine nanoparticle for relieving plant salt stress and a preparation method thereof. And adding the double-ligand chelated dopamine solution into the lignin-based nano-particle suspension, and stirring to obtain the lignin-based nano-particle suspension. According to the invention, metal ions are adopted as a connecting bridge and are respectively chelated with dopamine and ethylenediamine tetraacetic acid salt, so that double-ligand chelated dopamine is obtained, and the self-polymerization behavior of dopamine is inhibited. The invention also provides a method for loading dopamine by using lignin-based nanoparticles. The self-polymerization of the dopamine is further inhibited; the adsorption of the soil on the dopamine is reduced, and the utilization rate of the dopamine is improved.

The invention provides a polyimide multilayer composite membrane material as well as a preparation method and application thereof, and belongs to the technical field of gas separation. According to the method, through collaborative design of a functional support layer, a gradient MOF layer and an ultrathin selection layer structure, the problems of support layer pore seepage, MOF agglomeration, weak interface bonding and the like in an existing composite membrane material are solved. The preparation method comprises the following steps: firstly, carrying out electrostatic spinning on a polyamide acid glue solution, and synchronously introducing dopamine by adopting a chemical imidization method to prepare a functionalized polyimide fiber membrane containing hydroxyl and amino on the surface, so that the mechanical property of a supporting layer and the MOF anchoring capability are remarkably improved; growing MOF layers with different particle sizes on the surface of the fiber membrane by regulating and controlling the concentration gradient of ligand / metal salt and the reaction time, and passivating through a silane coupling agent after each step of growth so as to avoid heterogeneous nucleation and construct a gradient structure; after the MOF gradient layer is subjected to Ar plasma pretreatment to introduce hydroxyl, the ultrathin polyamide selection layer is formed by utilizing interfacial polymerization of fluorine-containing acyl chloride and a macrocyclic amine monomer, and the ultrathin polyamide selection layer is suitable for gas separation work.

The invention relates to a targeted drug delivery carrier based on mesoporous polydopamine nanoparticles and application thereof.The targeted drug delivery carrier is galactose modified mesoporous dopamine coordinated with manganese ions, lysine is adopted in the carrier synthesis step to provide an alkaline environment needed by dopamine polymerization, the polymerization process is simplified, and the drug delivery efficiency is improved. Meanwhile, lysine can be introduced into the mesoporous polydopamine; and the drug loading capacity of the carrier is improved. The carrier gathers multifunctionality of drug delivery, tumor imaging and treatment, provides a comprehensive new method for tumor treatment, and enhances individuation and precision of treatment.

The invention belongs to the technical field of micro-nano, and particularly relates to a drug-loaded microalgae motor and a preparation method and application thereof, and the drug-loaded microalgae motor comprises a polydopamine / ferroferric oxide / chlamydomonas reinhardtii micromotor and a drug loaded on the polydopamine / ferroferric oxide / chlamydomonas reinhardtii micromotor, the drug is combined with catechol groups on the surface of polydopamine in the polydopamine / ferroferric oxide / chlamydomonas reinhardtii micromotor through covalent bonds; the medicine is an anti-cancer medicine or an anti-inflammatory medicine. The microalgae motor has the characteristics of simple preparation method, good material biocompatibility, high biodegradability and the like. The polydopamine / ferroferric oxide / chlamydomonas reinhardtii drug-loaded micromotor prepared by the invention has the characteristic of self-driving in intestinal juice, so that the polydopamine / ferroferric oxide / chlamydomonas reinhardtii drug-loaded micromotor can target an intestinal disease part under the guidance of an external magnetic field to improve the treatment effect and reduce the side effect of a drug, thereby expanding the application space of a micro-nano motor in the field of biomedicine.

The invention discloses an OTX-015 (Ox-015) loaded cascaded responsive nano particle and a preparation method thereof. The nanoparticle has a three-layer core-shell structure, wherein the innermost layer is a cross-linked nanoparticle of chitosan and paclitaxel; the middle layer is a polydopamine layer loaded with a bromodomain inhibitor OTX-015; the outermost layer is a gelatin layer grafted with babali; wherein the cross-linked substance of the chitosan and the paclitaxel is a cross-linked substance containing a disulfide bond. The invention also provides a preparation method of the cascade responsive nano-particle and application of the cascade responsive nano-particle in preparation of an antitumor drug targeted delivery system. The nanoparticle provided by the invention improves the tumor microenvironment while activating in-vivo autoimmunity, and kills tumor cells by using photothermal therapy assisted chemotherapy drugs, so that the synergistic effect of chemotherapy / photothermal therapy / immunotherapy is realized, and a new idea is provided for clinical tumor treatment.

The invention discloses a hydrogel wound dressing with a synergistic healing promoting function as well as a preparation method and application thereof. The preparation method comprises the following steps: 1, dissolving oxidized methacrylated hyaluronic acid and dopamine in deionized water to obtain a solution A; the preparation method comprises the following steps: dissolving N-isopropylacrylamide, a photoinitiator 2-hydroxy-2-methyl-1-(4-(2-hydroxyethoxy) phenyl)-1-acetone and a cross-linking agent N, N '-methylene bisacrylamide in deionized water to obtain a solution B; 2, mixing the solution A and the solution B to obtain a pre-gel solution; or dissolving polyphenylboronic acid ester nanoparticles or polyphenylboronic acid ester-kaempferol with deionized water to obtain a solution C, and mixing the solution C with the solution A and the solution B to obtain a pre-gel solution; and 3, injecting the pre-gel liquid into a mold, and illuminating to obtain the hydrogel wound dressing loaded with the nanoparticles. The hydrogel disclosed by the invention can be used for effectively solving various problems in a wound healing process and promoting rapid and high-quality healing of a wound.

The invention discloses an application of a CD73 + NK cell in preparation of a medicine for preventing or treating Parkinson's disease. The applicant discovers that the CD73 + NK cell can prevent dopaminergic neuron loss and glial inflammation response of the MPP + induced VM cell in the experimental process. However, TGF-beta1 and IL-10 secreted by the CD73 + NK cells do not significantly prevent loss of dopaminergic neurons induced by MPP + in cross-pore co-culture of the CD73 + NK cells and VM neurons. Therefore, the CD73 + NK cells are proved to protect dopaminergic neurons from the influence of MPP + neurotoxicity through an intercellular contact mechanism, namely CD73-SIRPA interaction.

The invention discloses a brain-like pulse enhanced evolution unmanned obstacle avoidance method and a related device, and the method comprises the steps: generating an obstacle avoidance action at a current moment according to a state sequence of an unmanned vehicle at the current moment and a cortex-basal ganglion-thalamus network; and optimizing the cortex-basal ganglion-thalamus network at the next moment based on the obstacle avoidance action at the current moment, in the optimization process of the cortex-basal ganglion-thalamus network, according to the award difference value at the adjacent moments, accumulating the vigilance value of the cortex-basal ganglion-thalamus network to the environment, and when the accumulated vigilance value does not exceed a second threshold value, determining that the awarded value does not exceed the second threshold value. And when the accumulated vigilance value exceeds a first threshold value, optimizing the cortex-basal ganglion-thalamus network based on a peak time sequence dependency plasticity mechanism and a dopamine regulation mechanism in sequence, and when the accumulated vigilance value exceeds a second threshold value, optimizing the cortex-basal ganglion-thalamus network based on an offline evolution method of a genetic algorithm, the obstacle avoidance method which can quickly adapt to different complex environments and is accurate in decision making is realized.

The invention discloses novel application of notoginsenoside Rg1, namely application of the notoginsenoside Rg1 in preparation of a cathepsin D (CTSD) inhibitor, the notoginsenoside Rg1 can inhibit CTSD enzyme activity so as to recover lysosome structure and function, relieve endoplasmic reticulum stress reaction and relieve alpha-syn aggregation and dopaminergic neuron apoptosis, has a remarkable protection effect on a Parkinson's disease model, and can be used for preparing a novel anti-inflammatory drug. The invention provides a new thought and a potential drug basis for targeted intervention of Parkinson's disease. The invention discloses a new mechanism that Rg1 plays a role in neuroprotection through a CTSD-lysosome-endoplasmic reticulum stress pathway.

The invention relates to the technical field of targeted cargo delivery, in particular to a drug loading and delivery method and a cell carrying method of a core-shell mesoporous micro-nano robot. The drug loading and delivery method is realized based on the drug loading capacity of the mesoporous structure, the magnetism of the iron oxide core and the photothermal effect of the polydopamine shell. The core-shell mesoporous micro-nano robot can adsorb and load drugs through capillary force and electrostatic force generated by the mesoporous structure of the core-shell mesoporous micro-nano robot; then, an external magnetic field is used for driving the device to move; near-infrared light is utilized to irradiate when approaching a targeted area, the temperature of a pore structure rises, and along with thermal vibration, drugs in mesopores are effectively released to the targeted area, so that the drug delivery effect is achieved. When the drug is loaded and delivered, the core-shell mesoporous micro-nano robot has carrying capacity after being clustered under a magnetic field, and cells are carried through an eddy current carrying method and a contact adhesion method. Targeted goods delivery of goods such as targeted drugs and cells can be achieved through the micro-nano robot.

The invention provides a cheese protein hydrolysate as well as a preparation method and application thereof. The composition of the casein hydrolysate at least comprises at least one of a peptide fragment 1 with an amino acid sequence as shown in SEQ ID NO: 1, a peptide fragment 2 with an amino acid sequence as shown in SEQ ID NO: 2 and a peptide fragment 3 with an amino acid sequence as shown in SEQ ID NO: 3. The casein hydrolysate has good neuron protection and repair effects, and can improve cholinergic neuron and dopaminergic neuron damage; abeta protein deposition can be inhibited, so that neuronal synaptic disorder, neuronal apoptosis and brain injury caused by Abeta protein deposition are relieved; the cholinergic defect can be improved, so that the nerve development damage and the behavior and movement disorder caused by the cholinergic defect are relieved; and damaged neurons in hippocampus and hypothalamus can be repaired, so that the brain environment is kept stable.

The invention provides a hydrogel microneedle patch for treating oral ulcer and a preparation method, and belongs to the technical field of biomedical engineering. The technical problems that in the traditional oral ulcer administration process, medicine is difficult to retain at the focus for a long time, is easily scoured by saliva and is influenced by tongue and cheek movement are solved. According to the technical scheme, the method comprises the following steps: 1) designing and preparing a microneedle patch mold; (2) synthesizing drug-loaded polydopamine nano particles; (3) preparing a microneedle patch; and 4) evaluating the performance of the microneedle patch. The invention has the following beneficial effects: on one hand, the combination of the drug-loaded nanoparticles and the hydrogel microneedle array can reduce the dosage through drug sustained release; and on the other hand, the problems of high drug instability, low targeting property, poor bioavailability and hindering treatment effect are effectively solved, and the preparation can be widely applied to the fields of drug sustained release, wound healing, tissue engineering and the like.

The invention belongs to the technical field of electrode materials and nuclide recovery, and discloses a W18O49 / graphene / phosphorylation / amidoximation electrode material and preparation and application thereof. The preparation method comprises the following steps: dispersing ammonium tungstate and graphene in a trihydroxymethyl aminomethane solution, and heating and stirring for reaction to obtain a mixed solution; then adding dopamine hydrochloride, ethanol and ammonia water, stirring and reacting, and washing and drying a product to obtain a precursor; and carbonizing the obtained precursor in an inert atmosphere at the temperature of 600-900 DEG C to obtain a W18O49 / graphene material, then dispersing the W18O49 / graphene material in a hydroxylamine hydrochloride solution and / or a phytic acid solution for stirring reaction, and washing and drying the product to obtain the W18O49 / graphene / phosphorylation / amidoximation electrode material. The selective recovery efficiency of the obtained electrode material on uranyl ions can reach 95% or above, and the electrode material has wide application prospects in the field of nuclide uranium recovery.

The invention relates to the technical field of membrane separation, in particular to a composite forward osmosis membrane based on mussel bionic interface enhancement and a preparation method thereof. The preparation method comprises the following steps: immersing a base material in a dopamine hydrochloride solution for oscillation reaction to obtain a modified base material; the surface of the modified base material is coated with a membrane casting solution in a blade coating mode, then phase inversion is carried out, the residual solvent is removed, then airing is carried out, and the composite forward osmosis membrane based on mussel bionic interface enhancement is obtained. By introducing the mussel bionic polydopamine intermediate layer, the long-standing technical contradictions of poor interfacial compatibility and weak binding force between the hydrophobic substrate and the hydrophilic separation layer are ingeniously solved, and the internal concentration polarization effect is remarkably inhibited. The prepared composite membrane has the advantages of high water flux, low salt reverse flux, stable structure and the like, and has wide application prospects in the fields of water treatment and substance separation.

The invention discloses a compound traditional Chinese medicine patch for conditioning hyperplasia of mammary glands and a preparation method thereof, and relates to the technical field of traditional Chinese medicine preparations. The compound traditional Chinese medicine patch for conditioning hyperplasia of mammary glands is prepared from an iron-based metal organic framework composite drug carrying system, dopamine hydrochloride, a blank gel matrix, ethyl oleate, menthol, a solubilizer, a wetting agent and water, the iron-based metal organic framework composite drug loading system is composed of traditional Chinese medicine dihydric alcohol plastid, iron-based metal organic framework nanoparticles and cinnamomum camphora essential oil. The traditional Chinese medicine dihydric alcohol plastid and the cinnamomum camphora essential oil are loaded in the iron-based metal organic framework nano particles, so that the transdermal absorption of the medicine is improved, the pH response release property is realized, the dosage of active ingredients permeating into a focus part and the lasting action time are effectively increased, and then a coating layer is formed by polydopamine, so that the anti-inflammatory effect is improved. The dispersibility and compatibility of the drug loading system in vivo are further enhanced, the drug effect can be fully exerted, and the treatment effect on the hyperplasia of mammary glands is remarkably improved.

The invention provides an interface-enhanced regenerated bone scaffold for sustained-release medicines as well as a preparation method and application of the interface-enhanced regenerated bone scaffold, and belongs to the technical field of bone tissue engineering. According to the invention, polycaprolactone and hydroxyapatite are taken as matrix components of the regenerated bone scaffold, so that the regenerated bone scaffold has mechanical strength matched with a host bone and good biocompatibility; the polydopamine coating is loaded on the surface and internal pores of the regenerated bone scaffold matrix, surface functional modification is easy to perform, and a catechol structure in the polydopamine coating can perform coordination binding with metal ions in MOFs, so that the falling rate of MOFs particles is reduced, and the MOFs particles are uniformly and stably distributed; the MOFs particles are used for loading the eucommia ulmoides extract, safety and non-toxicity are achieved, the eucommia ulmoides extract is encapsulated in the MOFs particles, the stability of the eucommia ulmoides extract can be improved, and a better drug release effect and a better bone regeneration promoting effect are achieved.

The invention belongs to the technical field of medicines, and particularly relates to a bionic nano-drug for resisting subcutaneous drug-resistant bacterial infection as well as a preparation method and application thereof. Dopamine hydrochloride is added in the synthesis process of a metal-organic framework to prepare a dandelion-shaped carbon nano-enzyme precursor, then the nano-enzyme precursor is carbonized to obtain a carbon nano-enzyme with targeting property, photoresponse and peroxidase activity, and finally berberine hydrochloride is loaded on the carbon nano-enzyme to obtain the bionic nano-drug. The bionic nano-drug provided by the invention firstly has good biocompatibility, secondly has a unique dandelion-shaped form, is beneficial to playing a targeting role, and finally has relatively strong light response capability in a near-infrared region, and can synergistically and efficiently sterilize through action mechanisms of antibacterial drugs, photothermal therapy and chemical kinetics, so that the bionic nano-drug has a good application prospect. The compound has an important application value in preparation of drugs for treating subcutaneous drug-resistant bacterium infection.

The invention relates to a spatio-temporal sequence regulated dual stimulation responsive microneedle and a preparation method thereof, and the preparation method comprises the following steps: dispersing piezoelectric nanoparticles in a buffer solution, then adding dopamine hydrochloride, and carrying out a reaction to prepare polydopamine coated piezoelectric nanoparticles; the preparation method comprises the following steps: preparing a dispersion liquid from polydopamine-coated piezoelectric nanoparticles, adding the dispersion liquid into a temperature-sensitive hydrogel prepolymer liquid containing a hydrogel monomer and a drug, and uniformly mixing to obtain a mixed liquid; filling a microneedle mold with the mixed solution, standing for forming, and drying to obtain the dual stimulation responsive microneedle. According to the hydrogel microneedle, a temperature-sensitive material is adopted as a base material of the hydrogel microneedle, and polydopamine coated piezoelectric nanoparticles with photo-thermal and piezoelectric response are doped in the hydrogel microneedle, so that the release rate of a drug loaded in the hydrogel microneedle can be accelerated, and controllable release of the drug can be realized; and the piezoelectric nanoparticles can respond to mechanical stimulation and convert the mechanical stimulation into electric signals so as to synergistically promote tissue repair.

The invention provides a hydrogen bond organic framework material, a preparation method and application of the hydrogen bond organic framework material in dopamine detection, the preparation method of the hydrogen bond organic framework material comprises the following steps: S1, dissolving 1, 3, 5-tri (4-carboxyphenyl) benzene in an organic solvent to obtain an organic solution; and S2, dropwise adding an alcohol solvent into the organic solution to react to obtain turbid liquid, and drying to obtain the hydrogen bond organic framework material. The hydrogen bond organic framework material HOF-BTB prepared by the invention is a porous material, has two-dimensional (2D) pi-pi stacked hexagonal lamellas and complex eight interpenetration, generates a one-dimensional channel in the framework, and is simple in preparation process, mild in synthesis condition, low in cost, good in biocompatibility and low in cytotoxicity; and due to rigid organic construction molecules, the compound shows excellent fluorescence performance. The HOF-BTB material has high selectivity due to differential recognition of binding sites, and can be effectively applied to detection of dopamine.

The invention discloses pancreatic lipase immobilized composite nano magnetic beads, a preparation method and application, ferroferric oxide is adopted as a magnetic core, dopamine-mediated growth is performed to form a core-shell structure, then the periphery of the core-shell structure is wrapped with a metal organic framework to form the composite nano magnetic beads, and pancreatic lipase is immobilized by using the composite nano magnetic beads as an adsorption carrier. The enzyme loading amount, the repeated utilization rate and the storage stability of the ferroferric oxide nano-particles are remarkably improved, the enzyme immobilization efficiency and the enzyme activity are improved, and meanwhile, the prepared pancreatic lipase immobilized composite nano-magnetic beads have excellent stability and biocompatibility, are green and pollution-free and can be repeatedly used; the composite nano magnetic beads are synthesized by adopting a solvothermal method, the product is easy to separate, the synthesis process is simple, the enzyme immobilization efficiency can be regulated and controlled by regulating and controlling the synthesis process, the structure of an active ligand can be screened and analyzed from natural products with numerous and jumbled components, and the active ligand has a certain lipid-lowering potential and can be used for preparing the nano magnetic beads. The compound can be used as a potential lead compound of lipid-lowering drugs.