1520 results about "Dopamine" patented technology

The invention belongs to the technical field of biomedical materials, and particularly relates to adipose-derived mesenchymal stem cell loaded conductive hydrogel and a preparation method and application thereof, the preparation method comprises the following steps: step S1, after hyaluronic acid is subjected to oxidation treatment, dopamine is grafted to oxidized hyaluronic acid, and oxidized hyaluronic acid-dopamine is obtained; step S2, carrying out amination treatment on gelatin to obtain aminated gelatin; s3, mixing the oxidized hyaluronic acid-dopamine with the aminated gelatin, and carrying out Schiff base crosslinking, so as to obtain hydrogel; and step S4, loading the adipose tissue-derived stromal cells by using the hydrogel. Dopamine is grafted on an oxidized hyaluronic acid macromolecular chain through amidation reaction, the hydrophilicity and the electrical property of the macromolecular chain are improved, a catechol structure is provided to provide possibility for iron ion coordination, the tissue adhesion, the injectability, the mechanical property and the electrical conductivity of the hydrogel are improved, and the hydrogel can be applied to the field of biomedical materials. After being applied to a spinal cord injury rat model, the functional recovery can be promoted.

The invention relates to a preparation method and application of a Janus structure photo-thermal-piezoelectric hydrogel dressing for infected wound repair, and the preparation method comprises the following steps: respectively dissolving phenylboronic acid grafted methacrylamide gelatin and dopamine grafted hyaluronic acid in deionized water, adding MXene coated CeO2 heterojunction, and ultrasonically dispersing uniformly, so as to obtain the Janus structure photo-thermal-piezoelectric hydrogel dressing for infected wound repair. After mixing, sodium hydroxide is used for adjusting the pH value to alkalescence, and a Janus structure inner layer is formed; respectively dissolving phenylboronic acid grafted gelatin and o-nitrobenzyl alcohol grafted hyaluronic acid in deionized water, adding BaTiO3, ultrasonically dispersing uniformly, and mixing to form a Janus structure outer layer; the Janus structure photo-thermal-piezoelectric hydrogel dressing prepared by the invention has the characteristic of space-time release so as to adapt to the wound repair process, in addition, the Janus structure photo-thermal-piezoelectric hydrogel dressing also has excellent photo-thermal conversion performance, bacteria infecting the wound are killed through photo-thermal heating, physiological electric signals are effectively simulated through the piezoelectric effect, cell behaviors are regulated and controlled, and the Janus structure photo-thermal-piezoelectric hydrogel dressing can be used for wound infection repair.

The invention provides a composition for promoting hair regeneration after chemotherapy as well as a preparation method and application thereof, and belongs to the technical field of medicines. The preparation method comprises the following steps: modifying the surface of a gold nanocage with polydopamine, loading the modified gold nanocage on an exosome, complexing magnesium ions and zinc ions to prepare a modified exosome, preparing a nano drug-loaded liposome from curcumin, resveratrol and epigallocatechin gallate, mixing the nano drug-loaded liposome with the modified exosome to prepare a multi-layer liposome, and preparing the multi-layer liposome from the nano drug-loaded liposome. And mixing with a penetration enhancer, and adding into a temperature-sensitive hydrogel system to prepare the composition for promoting hair regeneration after chemotherapy. The composition for promoting hair regeneration after chemotherapy has a good transdermal absorption promoting effect, can slowly release drugs, promotes hair follicles to be converted from a resting stage to a growing stage under the synergistic effect of the drugs, regulates and controls multiple pathways (such as anti-apoptosis, angiogenesis promotion and anti-inflammation) at the same time, is matched with a complex pathological mechanism of hair regeneration after chemotherapy, and can promote hair regeneration after chemotherapy. Wide application prospects are realized.

The invention relates to the technical field of medical dressings, in particular to a perianal drug dressing of a multi-layer flow guide composite fabric and a preparation method and application of the perianal drug dressing of the multi-layer flow guide composite fabric. The perianal drug dressing is of a four-layer structure, a wet tissue adhesion layer is made of polydopamine modified chitosan / oxidized hyaluronic acid hydrogel, and wet-state adhesion is achieved; the drug sustained-release layer is used for dispersing mesoporous silica loaded with ciprofloxacin hydrochloride / defensin and chitosan microspheres loaded with epidermal growth factors / lonicera japonica into a polycaprolactone fiber membrane to achieve synergistic antibacterial and healing promotion; the directional flow guide layer is of a gradient wettability sandwich structure with a bionic micro-channel, and one-way rapid drainage can be achieved; the high-absorption outer layer is carboxymethyl cellulose / sodium polyacrylate composite sponge, liquid is locked efficiently, the wound surface is kept dry and comfortable, the clinical problem in perianal infection wound treatment can be effectively solved, and the treatment effect and the patient comfort are remarkably improved.

The invention discloses a double-crosslinking network hydrogel film for treating oral ulcer, a preparation method and application, and relates to the technical field of biomedical materials. According to the hydrogel film, a dual-network synergistic enhancement structure is constructed through covalent / non-covalent bi-crosslinking, the adhesion to oral mucosa is remarkably enhanced by adopting dopamine (DA) modified hyaluronic acid (HA), and the mechanical property is greatly improved by utilizing a bi-crosslinking network system of disulfide bond / double bond crosslinking (chitosan and CS) and hydrogen bond / catechol self-crosslinking (hyaluronic acid and HA); the antioxidant and anti-inflammatory characteristics of lipoic acid (LA) and the natural antibacterial property of chitosan (CS) are combined to play a synergistic effect, so that the antibacterial and anti-inflammatory effects are remarkably improved. Meanwhile, multifunctional modified materials such as metacrylic acid ester are introduced, so that the functionality and the application range of the hydrogel film are expanded. The preparation technology is optimized, it is ensured that the material is safe and effective, large-scale production is easy, and an efficient and reliable novel material is provided for oral ulcer treatment.

The invention provides a polyimide multilayer composite membrane material as well as a preparation method and application thereof, and belongs to the technical field of gas separation. According to the method, through collaborative design of a functional support layer, a gradient MOF layer and an ultrathin selection layer structure, the problems of support layer pore seepage, MOF agglomeration, weak interface bonding and the like in an existing composite membrane material are solved. The preparation method comprises the following steps: firstly, carrying out electrostatic spinning on a polyamide acid glue solution, and synchronously introducing dopamine by adopting a chemical imidization method to prepare a functionalized polyimide fiber membrane containing hydroxyl and amino on the surface, so that the mechanical property of a supporting layer and the MOF anchoring capability are remarkably improved; growing MOF layers with different particle sizes on the surface of the fiber membrane by regulating and controlling the concentration gradient of ligand / metal salt and the reaction time, and passivating through a silane coupling agent after each step of growth so as to avoid heterogeneous nucleation and construct a gradient structure; after the MOF gradient layer is subjected to Ar plasma pretreatment to introduce hydroxyl, the ultrathin polyamide selection layer is formed by utilizing interfacial polymerization of fluorine-containing acyl chloride and a macrocyclic amine monomer, and the ultrathin polyamide selection layer is suitable for gas separation work.

The invention discloses an OTX-015 (Ox-015) loaded cascaded responsive nano particle and a preparation method thereof. The nanoparticle has a three-layer core-shell structure, wherein the innermost layer is a cross-linked nanoparticle of chitosan and paclitaxel; the middle layer is a polydopamine layer loaded with a bromodomain inhibitor OTX-015; the outermost layer is a gelatin layer grafted with babali; wherein the cross-linked substance of the chitosan and the paclitaxel is a cross-linked substance containing a disulfide bond. The invention also provides a preparation method of the cascade responsive nano-particle and application of the cascade responsive nano-particle in preparation of an antitumor drug targeted delivery system. The nanoparticle provided by the invention improves the tumor microenvironment while activating in-vivo autoimmunity, and kills tumor cells by using photothermal therapy assisted chemotherapy drugs, so that the synergistic effect of chemotherapy / photothermal therapy / immunotherapy is realized, and a new idea is provided for clinical tumor treatment.

The invention discloses a hydrogel wound dressing with a synergistic healing promoting function as well as a preparation method and application thereof. The preparation method comprises the following steps: 1, dissolving oxidized methacrylated hyaluronic acid and dopamine in deionized water to obtain a solution A; the preparation method comprises the following steps: dissolving N-isopropylacrylamide, a photoinitiator 2-hydroxy-2-methyl-1-(4-(2-hydroxyethoxy) phenyl)-1-acetone and a cross-linking agent N, N '-methylene bisacrylamide in deionized water to obtain a solution B; 2, mixing the solution A and the solution B to obtain a pre-gel solution; or dissolving polyphenylboronic acid ester nanoparticles or polyphenylboronic acid ester-kaempferol with deionized water to obtain a solution C, and mixing the solution C with the solution A and the solution B to obtain a pre-gel solution; and 3, injecting the pre-gel liquid into a mold, and illuminating to obtain the hydrogel wound dressing loaded with the nanoparticles. The hydrogel disclosed by the invention can be used for effectively solving various problems in a wound healing process and promoting rapid and high-quality healing of a wound.

The invention discloses an application of a CD73 + NK cell in preparation of a medicine for preventing or treating Parkinson's disease. The applicant discovers that the CD73 + NK cell can prevent dopaminergic neuron loss and glial inflammation response of the MPP + induced VM cell in the experimental process. However, TGF-beta1 and IL-10 secreted by the CD73 + NK cells do not significantly prevent loss of dopaminergic neurons induced by MPP + in cross-pore co-culture of the CD73 + NK cells and VM neurons. Therefore, the CD73 + NK cells are proved to protect dopaminergic neurons from the influence of MPP + neurotoxicity through an intercellular contact mechanism, namely CD73-SIRPA interaction.

The invention discloses novel application of notoginsenoside Rg1, namely application of the notoginsenoside Rg1 in preparation of a cathepsin D (CTSD) inhibitor, the notoginsenoside Rg1 can inhibit CTSD enzyme activity so as to recover lysosome structure and function, relieve endoplasmic reticulum stress reaction and relieve alpha-syn aggregation and dopaminergic neuron apoptosis, has a remarkable protection effect on a Parkinson's disease model, and can be used for preparing a novel anti-inflammatory drug. The invention provides a new thought and a potential drug basis for targeted intervention of Parkinson's disease. The invention discloses a new mechanism that Rg1 plays a role in neuroprotection through a CTSD-lysosome-endoplasmic reticulum stress pathway.

The invention relates to the technical field of targeted cargo delivery, in particular to a drug loading and delivery method and a cell carrying method of a core-shell mesoporous micro-nano robot. The drug loading and delivery method is realized based on the drug loading capacity of the mesoporous structure, the magnetism of the iron oxide core and the photothermal effect of the polydopamine shell. The core-shell mesoporous micro-nano robot can adsorb and load drugs through capillary force and electrostatic force generated by the mesoporous structure of the core-shell mesoporous micro-nano robot; then, an external magnetic field is used for driving the device to move; near-infrared light is utilized to irradiate when approaching a targeted area, the temperature of a pore structure rises, and along with thermal vibration, drugs in mesopores are effectively released to the targeted area, so that the drug delivery effect is achieved. When the drug is loaded and delivered, the core-shell mesoporous micro-nano robot has carrying capacity after being clustered under a magnetic field, and cells are carried through an eddy current carrying method and a contact adhesion method. Targeted goods delivery of goods such as targeted drugs and cells can be achieved through the micro-nano robot.

The invention provides a hydrogel microneedle patch for treating oral ulcer and a preparation method, and belongs to the technical field of biomedical engineering. The technical problems that in the traditional oral ulcer administration process, medicine is difficult to retain at the focus for a long time, is easily scoured by saliva and is influenced by tongue and cheek movement are solved. According to the technical scheme, the method comprises the following steps: 1) designing and preparing a microneedle patch mold; (2) synthesizing drug-loaded polydopamine nano particles; (3) preparing a microneedle patch; and 4) evaluating the performance of the microneedle patch. The invention has the following beneficial effects: on one hand, the combination of the drug-loaded nanoparticles and the hydrogel microneedle array can reduce the dosage through drug sustained release; and on the other hand, the problems of high drug instability, low targeting property, poor bioavailability and hindering treatment effect are effectively solved, and the preparation can be widely applied to the fields of drug sustained release, wound healing, tissue engineering and the like.

The invention relates to the technical field of membrane separation, in particular to a composite forward osmosis membrane based on mussel bionic interface enhancement and a preparation method thereof. The preparation method comprises the following steps: immersing a base material in a dopamine hydrochloride solution for oscillation reaction to obtain a modified base material; the surface of the modified base material is coated with a membrane casting solution in a blade coating mode, then phase inversion is carried out, the residual solvent is removed, then airing is carried out, and the composite forward osmosis membrane based on mussel bionic interface enhancement is obtained. By introducing the mussel bionic polydopamine intermediate layer, the long-standing technical contradictions of poor interfacial compatibility and weak binding force between the hydrophobic substrate and the hydrophilic separation layer are ingeniously solved, and the internal concentration polarization effect is remarkably inhibited. The prepared composite membrane has the advantages of high water flux, low salt reverse flux, stable structure and the like, and has wide application prospects in the fields of water treatment and substance separation.

The invention provides an interface-enhanced regenerated bone scaffold for sustained-release medicines as well as a preparation method and application of the interface-enhanced regenerated bone scaffold, and belongs to the technical field of bone tissue engineering. According to the invention, polycaprolactone and hydroxyapatite are taken as matrix components of the regenerated bone scaffold, so that the regenerated bone scaffold has mechanical strength matched with a host bone and good biocompatibility; the polydopamine coating is loaded on the surface and internal pores of the regenerated bone scaffold matrix, surface functional modification is easy to perform, and a catechol structure in the polydopamine coating can perform coordination binding with metal ions in MOFs, so that the falling rate of MOFs particles is reduced, and the MOFs particles are uniformly and stably distributed; the MOFs particles are used for loading the eucommia ulmoides extract, safety and non-toxicity are achieved, the eucommia ulmoides extract is encapsulated in the MOFs particles, the stability of the eucommia ulmoides extract can be improved, and a better drug release effect and a better bone regeneration promoting effect are achieved.

The invention discloses pancreatic lipase immobilized composite nano magnetic beads, a preparation method and application, ferroferric oxide is adopted as a magnetic core, dopamine-mediated growth is performed to form a core-shell structure, then the periphery of the core-shell structure is wrapped with a metal organic framework to form the composite nano magnetic beads, and pancreatic lipase is immobilized by using the composite nano magnetic beads as an adsorption carrier. The enzyme loading amount, the repeated utilization rate and the storage stability of the ferroferric oxide nano-particles are remarkably improved, the enzyme immobilization efficiency and the enzyme activity are improved, and meanwhile, the prepared pancreatic lipase immobilized composite nano-magnetic beads have excellent stability and biocompatibility, are green and pollution-free and can be repeatedly used; the composite nano magnetic beads are synthesized by adopting a solvothermal method, the product is easy to separate, the synthesis process is simple, the enzyme immobilization efficiency can be regulated and controlled by regulating and controlling the synthesis process, the structure of an active ligand can be screened and analyzed from natural products with numerous and jumbled components, and the active ligand has a certain lipid-lowering potential and can be used for preparing the nano magnetic beads. The compound can be used as a potential lead compound of lipid-lowering drugs.

The invention discloses a mesenchymal stem cell membrane bionic nano-enzyme for targeted therapy of Parkinson's disease as well as a preparation method and application of the mesenchymal stem cell membrane bionic nano-enzyme. The mesenchymal stem cell membrane bionic nano enzyme comprises an inner core and a mesenchymal stem cell membrane, wherein the inner core is composed of mesoporous polydopamine nanoparticles, and the mesenchymal stem cell membrane coats the inner core. The medicine contains the mesenchymal stem cell membrane bionic nano enzyme. The mesenchymal stem cell membrane bionic nano-enzyme disclosed by the invention shows good biocompatibility, and can be homed to a Parkinson disease region in the brain under the mediation of CXCR4 and VLA-4 specific membrane proteins. In addition, through the CD47 membrane protein expressed on the surface, the removal of the immune system can be avoided, so that the action time in the body is prolonged. The nano-enzyme is helpful for eliminating reactive oxygen free radicals, reducing oxidative stress level and reducing damage of neuroinflammation to dopaminergic neurons, so that safe and efficient treatment of Parkinson's disease is realized.

The invention provides a fabric-reinforced electroactive nerve conduit and a preparation method and application thereof, and belongs to the field of biomedical materials.The fabric-reinforced electroactive nerve conduit comprises a nerve conduit inner layer and a nerve conduit outer layer, the nerve conduit inner layer is a woven skeleton formed by magnesium wires serving as parallel yarns and degummed silk through a three-dimensional weaving process, the pore diameter of the nerve conduit inner layer is 1.5-2 mm, and the thickness of the nerve conduit inner layer is 0.5-1 mm; the outer layer of the nerve conduit is a composite layer with a radial finger-shaped porous structure, which is formed by directionally freezing and casting a hydrogel cross-linked network formed by mixing potassium sodium niobate nanorods modified by polydopamine and a biopolymer material, and the thickness of the outer layer of the nerve conduit is 1-2mm. A three-dimensional weaving technology, a piezoelectric nano material and a biopolymer material are combined, a novel multifunctional nerve conduit is developed to repair nerve injury, a better solution is provided for nerve repair, and the fabric enhanced electric activity nerve conduit has important practical guiding significance in application of future clinical nerve injury repair.

The invention constructs a universal visual virus molecularly imprinted sensor modified with an anti-protein coating, the sensor uses magnetic Fe3O4 as an imprinting carrier, HBV as a template and dopamine as a functional monomer for imprinting to obtain a molecularly imprinted polymer with a polydopamine coating (PDA), and before the template is removed, the molecular imprinting polymer with the polydopamine coating (PDA) is separated from the template, so that the molecular imprinting polymer with the polydopamine coating (PDA) is obtained. The surface of the PDA layer is covered with a slightly cross-linked PMEO2MA layer through a water precipitation polymerization method, and the polymer can effectively reduce non-specific protein adsorption; and finally, exposing an imprinting cavity of the PDA layer by eluting the template molecules. On the other hand, potassium permanganate (KMnO4) is used as a signal output molecule, and when no target object exists, the strong reducibility of PDA can enable KMnO4 entering the imprinting cavity to be subjected to a color fading reaction. Based on the principle, high-specificity colorimetric visual detection on the HBV is realized. The sensor can be combined with a smart phone through naked eyes to carry out visual semi-quantitative analysis and carry out quantitative detection through ultraviolet rays, and the universality of the sensor can be confirmed through a template replacement experiment. And meanwhile, the sensor shows an excellent cost advantage. The diagnosis which is high in sensitivity, low in cost and capable of achieving rapid visualization shows huge potential in practical application, and particularly has important application value in infectious disease screening and monitoring work in areas with limited resources.

The invention discloses a dopamine sensor working electrode based on an MOF material and a preparation method thereof, and belongs to the technical field of electrochemical sensing. The working electrode is composed of a pure MOF Cu-HHTP modified glassy carbon electrode and a Nafion film covering the surface of the glassy carbon electrode, and the preparation method of the working electrode comprises the following steps: synthesizing Cu-HHTP (Cu < 2 + > and a hexahydroxy triphenyl ligand are coordinated to form a two-dimensional honeycomb layer) through a solvothermal method, dispensing Cu-HHTP dispersion liquid on the surface of the glassy carbon electrode, and then covering the glassy carbon electrode with the Nafion film to form the combined electrode. The dopamine sensor prepared by the preparation method disclosed by the invention has high sensitivity, high anti-interference capability and long-term stability. The preparation process is simple, complex conductive additives are not needed, and the method is suitable for rapid and accurate detection of dopamine in complex biological samples.

The invention discloses a Parkinson's disease animal model, a construction method and application, and relates to the field of animal genetic engineering and disease model construction. According to the model, seven mutation sites related to the human familial Parkinson's disease are introduced behind an initiation codon of a fourth exon of an endogenous SNCA gene, and the mutation sites are A18T, A29S, A30P, E46K, H50Q, G51D and A53T and have genotypes related to the human familial Parkinson's disease. The pig model shows nigra dopaminergic neuron reduction and cortical neuron reduction in the newborn period, and the core pathological process of the human Parkinson's disease can be systematically reproduced. The model can be widely applied to Parkinson's disease pathogenesis research, drug screening and cell therapy effect evaluation, and has important scientific research and preclinical application values.

According to the scheme, the expanded polytetrafluoroethylene artificial blood vessel with the anticoagulant coating and preparation and application are provided, firstly, groups such as carboxyl and amino are formed on the inner surface of the expanded polytetrafluoroethylene artificial blood vessel through co-auto-polymerization reaction of L-dopamine and polyethyleneimine in a Tris aqueous alkali, then epoxy chloropropane serves as a cross-linking agent, and the expanded polytetrafluoroethylene artificial blood vessel with the anticoagulant coating is prepared. Trihydroxymethyl methylamino propanesulfonic acid is cross-linked to the inner surface of the membrane, carboxyl and sulfonic acid groups on the surface of the membrane endow the expanded polytetrafluoroethylene artificial blood vessel with anticoagulation performance, meanwhile, the hydrophobic performance of the outer surface is reserved, anticoagulation finishing is conducted on the inner wall of the expanded polytetrafluoroethylene artificial blood vessel, and the hydrophobic performance of the outer wall of the blood vessel is reserved; the expanded polytetrafluoroethylene artificial blood vessel has an anticoagulation function and does not influence the growth of fiber cells and fiber connective tissues on the surface of the blood vessel.

The invention relates to an electrochemical sensor for monitoring beta cell insulin secretion in real time and a preparation method and application thereof, the sensor is an electrochemical chip modified by a multi-dimensional nano material, and the multi-dimensional nano material on the electrochemical chip is used for capturing dopamine released by beta cells; and determining the release quantity of dopamine according to the electrochemical signal. According to the method, the insulin secretion condition of the beta cells is monitored in real time by taking dopamine as a tracer agent, and the exocytosis process of the beta cells can be researched under natural and normal conditions. According to the invention, only a common Faraday cage and a basic electrochemical workstation are needed for detection, one or more beta cells can be detected, the operation is simple, and the research efficiency of insulin secretion is greatly improved. In addition, the medicine for regulating beta cell insulin secretion can be efficiently screened, and a foundation is laid for research of diabetes treatment medicine.

Provided are compounds of Formula I and Formula II, described herein, processes for their preparation, their use as pharmaceuticals, and pharmaceutical compositions comprising them and intermediates used in their preparation. Compounds of Formula I and Formula II are useful, for instance, in modulating dopamine and serotonin neurotransmission and treating disorders that may benefit from the same, such as schizophrenia and depression.

The invention relates to the technical field of water treatment filter materials, in particular to an intelligent clean gradient composite water purification filter element and a preparation method thereof. The filter element adopts an integrated design of'natural gradient base material + ZIF-67 function enhancement + fluorescence-ultrasonic intelligent regeneration ': modified vegetable sponge is used as a porous support layer, a silica gel / ZIF-67 composite gradient layer is constructed on the porous support layer to realize adsorption and antibacterial dual functions, and dopamine-aluminum ions are used for composite surface modification to enhance hydrophilicity and interlayer binding force; a matched automatic system senses pollution in real time through the fluorescence monitoring module and triggers the ultrasonic regeneration module to complete self-cleaning, and the flux recovery rate is larger than or equal to 97.5%; the interception rate of the filtering element to anabaena under the operation pressure of 0.1-0.3 MPa is larger than or equal to 99.2%, the filtering flux is increased by 6-8 times compared with that of a commercial element, the filtering element can tolerate complex water quality in the coexistence of drugs and personal care products, natural vegetable sponge serves as a base material, and the filtering element has the advantages of biocompatibility and low cost and is suitable for drinking water purification and eutrophication water body treatment.

The invention belongs to the technical field of microbial fuel cells, and particularly relates to a microbial fuel cell, a carbon-based anode and a preparation method of the carbon-based anode. Preparing a polyaniline / dopamine modifier from an aniline monomer and dopamine hydrochloride, and modifying the polyaniline / dopamine modifier on a carbon felt electrode to form a PANI-DA / CF anode; the preparation method comprises the following steps: preparing flower-like Fe2O3 nanoparticles by adopting a hydrothermal method, and electrochemically depositing the flower-like Fe2O3 nanoparticles on a PANI-DA / CF electrode to prepare a Fe2O3 / PANI-DA / CF anode; according to the microbial fuel cell disclosed by the invention, the maximum power density of the phenol wastewater anolyte reaches 4594 + / -97 mW / m < 2 > and is improved by 83.1% compared with that of a carbon felt anode MFC (Microbial Fuel Cell); after operation for 96 hours, the phenol degradation rate reaches 99.4%; and the electricity generation performance is still kept stable after three months of operation. The Fe2O3 / PANI-DA / CF anode provided by the invention improves the power density output of the microbial fuel cell and the wastewater treatment efficiency.

The invention relates to the technical field of environmental health risk evaluation, in particular to a method for evaluating the risk of Parkinson's disease induced by environmental pollutants. The method for evaluating the risk of inducing the Parkinson's disease by the environmental pollutants comprises the following steps: carrying out a subacute exposure experiment by taking caenorhabditis elegans as a model organism; observing the transport capacity of the caenorhabditis elegans, observing and counting the abnormal movement ability of the caenorhabditis elegans, and determining the lipofuscin level of the caenorhabditis elegans, the damage degree of dopaminergic neurons, the dopamine level and the expression and aggregation conditions of alpha-synuclein protein, so as to evaluate the risk of the Parkinson's disease induced by environmental pollutants; the environmental pollutant is 6: 2 chlorinated polyfluoroalkyl ether sulfonate. According to the method, the PD toxicity evaluation model is generated by constructing a behavior characteristic and molecular characteristic scheme and combining multi-strain nematode data, and the problem that in the prior art, the risk of inducing PD by environmental pollutants is difficult to comprehensively reveal due to the fact that a single dimension is concerned is solved.

The invention provides a woven nerve conduit with piezoelectric performance and a preparation method and application thereof, and belongs to the field of nerve conduits.The preparation method comprises the steps that degummed silk is soaked in a dopamine hydrochloride solution, sodium hydroxide is added to adjust the pH value, and stirring is conducted to obtain polydopamine modified degummed silk; dispersing the polydopamine modified degummed silk, EDOT and FeCl3. 6H2O in an ethanol solution, stirring, centrifuging, washing and drying to obtain PEDOT modified degummed silk; the degummed silk modified by PEDOT is used as weaving yarn, and a nerve conduit skeleton is woven through a three-dimensional weaving technology; preparing a glycine solution, crystallizing and grinding to obtain beta-type glycine; pLLA particles are dispersed in hexafluoroisopropanol to be dissolved, beta-type glycine is added to obtain a PLLA-coated Gly solution, a PLLA-coated Gly fiber membrane is prepared on the surface of a nerve conduit skeleton through electrostatic spinning, and the woven nerve conduit with the piezoelectric property is obtained. The novel nerve conduit is developed by combining the three-dimensional weaving technology and the piezoelectric material, and a better solution is provided for nerve restoration.

The invention provides a cation exchange membrane chromatography medium and a preparation method and application thereof, the cation exchange membrane chromatography medium comprises a homo-porous membrane, polydopamine is loaded on two side surfaces and in a pore structure of the homo-porous membrane, and a cation exchange functional ligand is grafted on the polydopamine; a homogeneous pore membrane is selected as a matrix membrane, polydopamine is loaded on the surface and in an internal pore structure of the homogeneous pore membrane, and then a cation exchange functional ligand is further grafted, so that the surface of a chromatography medium of the obtained cation exchange membrane has relatively strong negative charges, and biomolecules with positive charges can be effectively adsorbed, thereby being separated from biomolecules with negative charges; meanwhile, the membrane is relatively low in transmembrane pressure and relatively high in separation degree, also has the characteristics of high permeability and high selectivity, and is suitable for being applied to separation and purification of biological drug macromolecules.

The invention is applicable to the technical field of hydrogel, and provides dynamic cross-linked hydrogel and a preparation method and application thereof, and the preparation method comprises the following steps: cross-linking oxidized dextran and carboxymethyl chitosan containing phenylboronic acid modification groups; glucanase is added before the hydrogel is formed, and calcium peroxide nanoparticles coated with polydopamine are introduced; wherein the mass concentration of the oxidized dextran is 2-10% w / v, the mass concentration of the carboxymethyl chitosan containing the phenylboronic acid modified group is 2% w / v, the concentration of the glucanase is 10-100 U, and the concentration of the calcium peroxide nanoparticles coated with the polydopamine is 0.1-1% w / v. The hydrogel disclosed by the invention shows good cell compatibility, angiogenesis promoting capability and bioactivity for promoting dental pulp regeneration in multiple in-vitro and in-vivo experiments, is suitable for tissue engineering fields such as dental pulp injury repair and the like, and effectively solves the technical problem that conventional hydrogel is insufficient in cell survival support in three-dimensional culture and the like.

The invention discloses a polydopamine modified metatitanic acid type lithium ion sieve and a preparation method thereof, and belongs to the technical field of lithium ion sieves, the preparation method comprises the following steps: grinding and uniformly mixing lithium acetate dihydrate and titanium dioxide in an organic solvent, and calcining the mixture at 650-800 DEG C for 3-5 hours to prepare a Li2TiO3 precursor; the preparation method comprises the following steps: putting dopamine hydrochloride, aminated crown ether and a Li2TiO3 precursor into a tris (hydroxymethyl) aminomethane solution with the pH value of 8-9, carrying out a constant-temperature oscillation reaction, carrying out suction filtration, and drying at low temperature, so as to obtain the polydopamine modified metatitanic acid type lithium ion sieve. According to the lithium ion sieve, the adsorption effect on lithium ions can be improved, the lithium extraction efficiency is improved, and the problem of low adsorption capacity of a lithium ion sieve in the prior art is solved.