152 results about "Novel gene" patented technology

The invention discloses application of TaMYB-7B and a coding gene thereof in regulation and control of wheat tiller number, and belongs to the technical field of gene engineering. The invention finds for the first time that the TaMYB-7B gene is a novel gene capable of simultaneously regulating and controlling the tillering number and grain traits of wheat. The TaMYB-7B gene is interfered, knocked out, silenced or mutated, so that the tiller number of a plant can be increased, the grain length is increased, the hundred-grain weight is increased, finally, the yield of a single wheat plant can be increased, and a foundation is laid for wheat yield improvement and character improvement.

The invention discloses application and a method of wheat demethylase TaALKBH23 in regulation and control of drought stress resistance of plants, a coding sequence of the demethylase TaALKBH23 is shown as SEQ ID NO: 1, and an amino acid sequence coded by the demethylase TaALKBH23 is shown as SEQ ID NO: 2. The invention provides demethylase TaALKBH23 and a function and application of a coding gene of the demethylase TaALKBH23 in regulation and control of plant drought resistance, and it is found through construction of a TaALKBH23 wheat genetic transformation material that the gene negatively regulates and controls wheat drought resistance. It is found that TaALKBH23 plays an important role in the process of regulating and controlling drought stress resistance of plants, and a new gene resource is provided for improving drought resistance of crops.

The invention discloses a sugarcane ScPP2C49 gene and application thereof, and relates to the technical field of plant biology, the nucleotide sequence of the ScPP2C49 gene is as shown in SEQ ID No.1, and the amino acid sequence of protein coded by the ScPP2C49 gene is as shown in SEQ ID No.2. The gene is induced to express by drought and abscisic acid, and the encoded protein of the gene is positioned in a cell nucleus. Functional studies show that after overexpression of the ScPP2C49 gene in the plant, the drought resistance of the plant is negatively regulated through various mechanisms of inhibiting an abscisic acid signal channel, hindering stomatal closure under drought stress, weakening active oxygen scavenging ability, reducing photosynthetic efficiency, inhibiting root growth and the like. The invention also provides a recombinant overexpression vector containing the gene and a method for obtaining a transgenic plant with reduced drought resistance by using the vector through an agrobacterium-mediated method. According to the invention, a new gene resource is provided for deep analysis of a plant drought-resistant molecular mechanism, and an important target gene is provided for cultivation of high-stress-resistance crop varieties through a reverse genetics means (such as gene knockout).

In order to control plant diseases caused by Xanthomonas bacteria, a novel bacteriophage exhibiting bacteriolytic activity specific to the same bacteria is isolated, and a plant disease control composition containing the same as an active ingredient is developed and provided. Provided are a lysozyme containing a bacteriophage having a novel genomic DNA sequence and exhibiting a lysolytic activity specific to Xanthomonas bacteria, and a plant disease control composition containing the lysozyme as an active ingredient.

The application discloses application of a rice disease resistance related gene Os04g55720 in bacterial blight breeding and relates to the technical field of modern biotechnology and genetic engineering technology.The rice disease resistance related gene Os04g55720 has a nucleotide sequence as shown in SEQ ID NO.1, is composed of 2821 nucleotides, has a coding region sequence as shown in SEQ ID NO.2, and has a sequence of 2148 nucleotides; the gene encodes a protein with 613 amino acids, and has an amino acid sequence as shown in SEQ ID NO.3.The application proves that overexpression of Os04g55720 enhances the resistance of rice to bacterial blight by means of molecular biology and biochemical technology, and shows that the rice protein OsPGDH-3 can effectively control the incidence of rice bacterial blight at a relatively low level.The application provides a new idea, strategy and gene resource for green prevention and control of rice bacterial blight.

The application discloses application of a corn gene in improving corn kernel protein content, and relates to the technical field of biology. ZmAAP16 A new gene capable of improving corn kernel content is mined by the application. ZmAAP16 Through transgenic method, function of the gene is analyzed and identified in depth, it is found that the gene can significantly improve the corn kernel protein content by overexpression in plants, and the role of the gene in improving the corn kernel protein content is determined. The application has important significance for cultivation of high-protein ensiled corn.

The application discloses application of a PHB9 gene in regulating rice leaf morphology and belongs to the technical field of genetic engineering.The CDS sequence of the PHB9 gene is shown as SEQ ID NO.2.It is found through experiments that overexpression of the PHB9 gene increases leaf length and leaf width, and the PHB9 gene has a significant effect on regulating rice leaf morphology.Further, changes in vascular systems, mitochondria and chloroplast structures in the internal structure of the leaf are analyzed, and it is shown that the vascular systems, mitochondria and chloroplast structures change when the leaf morphology is regulated, and the morphological changes in the internal structure of the leaf affect the changes in the leaf phenotype, and have a significant influence on improving the photosynthetic rate of the leaf.It is also found that overexpression of the PHB9 gene reduces the sensitivity of rice seeds to ABA, promotes seed germination and root growth.The application provides a new gene for breeding of leaf morphology building, root development and seed germination.

Provided is an invention based, in part, on novel gene constructs that encode a microdystrophin protein for use in gene therapy. The microdystrophin gene constructs and expression cassettes were engineered for improved therapy with respect to efficacy, potency and safety to the subject when expressed by a viral vector in muscle cells and / or CNS cells.

This invention relates to the field of biotechnology, specifically to the application of the ZmSKI3 and ZmSKI2 genes in regulating maize kernel development. This invention is the first to identify the ZmSKI3 and ZmSKI2 genes in maize and confirms that both genes control maize kernel development. Specifically, inhibiting the expression of ZmSKI3 and ZmSKI2 genes can delay maize kernel development. Therefore, this invention provides two key regulatory factors affecting maize kernel development, offering important molecular targets for improving maize kernel yield. Furthermore, this invention provides a novel gene resource for regulating maize kernel development; future manipulation of these genes can yield new germplasm with improved kernel development, enabling widespread application in production.

The application belongs to the technical field of biology, and discloses application of CmPHL1 transcription factor in inhibition of citrus leaf oil cell formation and regulation of volatile substances. In view of the problem of insufficient research on existing citrus oil cell formation and essential oil synthesis, a pGBI-CmPHL1 overexpression vector is constructed, and positive strains are obtained by means of Agrobacterium-mediated transformation of citrus. Experiments prove that overexpression of CmPHL1 can significantly reduce the number of oil cells and the content of monoterpenes, sesquiterpenes and other volatile substances. CmPHL1 targets the molecular regulation network of oil cell development, accurately regulates and is heritable, provides a new gene target for citrus quality improvement and breeding, is in line with the direction of green agriculture, and has important economic and ecological benefits.

The invention discloses application of a TaPP2CL-A gene in regulation and control of wheat tiller number, and belongs to the technical field of plant antiviral gene engineering. The invention discloses a novel gene TaPP2CL-A for regulating and controlling the tiller number of wheat. Wheat genetic transformation experiments and further phenotypic analysis find that compared with wild Fielder, the tiller number of a TaPP2CL-A overexpressed plant is reduced, and finally the yield of a single plant is reduced; the tiller number of a TaPP2CL-A silent plant is increased, so that the yield of a single plant is increased. The TaPP2CL-A gene provided by the invention can be used in the field of transgenic breeding, and can lay a theoretical foundation for wheat yield increase and character improvement.

The invention discloses application of a LOXL2 gene in resisting avian leukosis virus infection, and belongs to the technical field of biology. The nucleotide sequence of the LOXL2 gene is as shown in SEQ ID NO. 1. The invention provides a new gene target LOXL2 related to avian leukosis virus infection resistance, and proves the effect of the LOXL2 gene in regulating and controlling avian leukosis virus replication. Replication of the avian leukemia virus can be promoted by inhibiting expression of the LOXL2 gene in a host cell, and replication of the avian leukemia virus can be inhibited by overexpressing the LOXL2 gene in the host cell. According to the invention, favorable theoretical guidance and technical support are provided for the application of the LOXL2 gene as a target in the aspects of development of drugs for treating avian leukemia, breeding of transgenic animals for resisting avian leukemia, construction of avian leukemia virus infection models and the like, and the LOXL2 gene has wide application prospects and extremely high market value.

The present application belongs to the technical field of plant genetic engineering, and particularly relates to a Phyllostachys edulis PeMASR1 gene, biological material and application thereof. The PeMASR1 gene provided by the present application is a new gene discovered in the study of cold tolerance of Phyllostachys edulis leaves, the nucleotide sequence of which is shown as SEQ ID NO:1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO:2. Through gene cloning, expression and comparative analysis, the phylogenetic position of the Phyllostachys edulis PeMASR1 gene and the important role thereof in the research of plant response to abiotic stress mechanism and photosynthesis are revealed. Overexpression in Columbia-type Arabidopsis plants can make the rosette leaves of the plants early senesce; and the plants show a more stress-tolerant phenotype than wild-type Arabidopsis in response to cold stress, drought stress and salt stress and other abiotic stresses.

The application provides a novel gene for regulating plant insect resistance and application thereof. Based on large-scale research screening and experimental work, a novel gene MYB22 related to regulating plant insect resistance is cloned, and the loss of function of the gene MYB22 leads to a great decrease in the insect resistance of plants, which indicates that the gene MYB22 is a gene with insect resistance function. The application provides a novel way for improving the insect resistance of plants.

The application provides application of Brassica napus gene BnDTX19 in sclerotinia prevention and control, and is obtained by creating transgenic rapeseed to change the disease resistance of rapeseed material. The application constructs the super-expression and RNAi transgenic rapeseed of BnDTX19, firstly discloses the positive regulation function of the gene on sclerotinia resistance, provides the application of the BnDTX19 gene in obtaining high-sclerotinia-resistant rapeseed material by creating super-expression rapeseed, and the application of the BnDTX19-RNAi rapeseed in obtaining sclerotinia-resistant rapeseed material with weakened sclerotinia resistance. The BnDTX19 gene product provided by the application has the function of transporting the key pathogenic factor oxalic acid of sclerotinia to promote disease resistance, and is a new gene resource suitable for creating and breeding new sclerotinia-resistant rapeseed materials and new varieties.

This invention discloses a method and application for improving wheat drought stress resistance based on the transcription factor TaHY5, relating to the fields of plant genetic engineering, wheat genetic breeding, and crop stress resistance improvement. This invention involves constructing a TaHY5 overexpression vector or a CRISPR / Cas9 editing vector, and transforming wheat immature embryos / callus tissue using Agrobacterium-mediated transformation or gene gun methods to obtain transgenic and gene-edited materials. After drought stress treatment, indicators such as biomass, malondialdehyde (MDA) content, and electrolyte permeability are measured to comprehensively evaluate drought resistance. Results show that TaHY5 overexpression significantly increases wheat biomass, APX, CAT and other antioxidant enzyme activities, as well as soluble protein and GSH content under drought stress, while reducing MDA accumulation and electrolyte permeability, and enhancing photosynthetic maintenance, reactive oxygen species scavenging, osmotic regulation, and cell membrane stability. This invention is the first to demonstrate that TaHY5 can synergistically improve wheat drought resistance, providing a simple and comprehensive improvement scheme, and offering new gene resources and practical technical pathways for wheat drought-resistant breeding.

The invention relates to application of DNA polymerase in long-fragment precise gene editing and construction in a plurality of human-derived cell lines. The invention belongs to the technical field of biological medicine. A novel gene editing system MMEJ-Pol theta is created by applying DNA polymerase Pol theta and a key functional structure domain, the accuracy, safety and editing efficiency of long-fragment gene editing in a eukaryotic genome are remarkably improved through a micro homologous end linking method, and an effective tool is provided for transformation application of the gene editing system in gene therapy and cell therapy.

The application provides a ZNF467 gene mutant and application thereof, and the mutant sequence is shown as SEQ ID NO. 1. The application discloses a novel 216-292 amino acid truncated mutant of a gene ZNF467 which is specifically enriched in human hematopoietic stem cells. Overexpression of the mutant can significantly improve the homing and transplantation efficiency of the human hematopoietic stem cells, and has great significance for the clinical treatment of diseases related to hematopoietic stem cell transplantation.

Compositions and methods for conferring nematicidal activity upon bacteria, plants, plant cells, tissues, and seeds are provided. Methods for killing or controlling populations of nematode pests, such as cyst nematode species, for example, *Heterodera glycines* (soybean cyst nematode), root-knot nematodes, kidney-shaped nematodes, short-bodied nematode species, or lanceolate nematode populations, are also provided. These methods further include contacting the nematode pest with a pest-killing amount of a polypeptide containing a nematicidal toxin. Further methods include increasing plant yield by expressing genes of the embodiments in plants.

The invention discloses a group of glycosyl transferase genes and application of the glycosyl transferase genes in preparation of mogroside, the glycosyl transferase genes are two new glycosyl transferase genes obtained by mining in a siraitia grosvenorii genome for the first time, are respectively named as UGT153033 and UGT30033, and have nucleotide sequences as shown in SEQ ID NOs: 1 and 2; meanwhile, the invention discloses glycosyl transferase coded by the glycosyl transferase gene and a mutant of the glycosyl transferase, wherein the glycosyl transferase and the mutant are respectively shown as SEQ ID NOs: 3-11; meanwhile, the invention also discloses a nucleic acid construct or an expression vector containing the gene or expressing the glycosyl transferase, and a host cell transformed by the nucleic acid construct or the expression vector. A new gene is provided for artificially synthesizing mogroside monomers, and the defects that the number of genes related to existing mogroside biosynthesis is small, and the molecular synthesis mechanism of most mogroside compounds is not clear yet are overcome.

The application discloses application of a LOXL2 gene in resisting avian leukemia virus infection and belongs to the technical field of biotechnology.The nucleotide sequence of the LOXL2 gene is shown as SEQ ID NO.1.The application provides a new gene target point LOXL2 related to resisting avian leukemia virus infection, and proves the role of the LOXL2 gene in regulating avian leukemia virus replication.Inhibition of LOXL2 gene expression in host cells can promote avian leukemia virus replication, and overexpression of the LOXL2 gene in host cells can inhibit avian leukemia virus replication.The application provides favorable theoretical guidance and technical support for application of the LOXL2 gene as a target point in developing drugs for treating avian leukemia, breeding transgenic animals resisting avian leukemia, and constructing an avian leukemia virus infection model, and has a wide application prospect and extremely high market value.

Plants, cells, tissues, and germplasms comprising genes and labeled alleles associated with increased resistance to gray leaf spot are provided. Also provided are methods of breeding plants having alleles associated with increased resistance to gray leaf spot and methods of identifying and selecting plants having alleles associated with increased resistance to gray leaf spot. Methods for identifying novel genes encoding proteins that provide resistance to gray leaf spot in plants and uses thereof are provided. The genes and marker alleles of the present disclosure can be used to produce gray leaf spot resistant plants by breeding, transgenic modification or genome editing.

This invention provides a gene prediction and identification method, apparatus, device, and storage medium, belonging to the field of gene annotation and prediction. The method includes: acquiring the gene text to be annotated and a set of promoter-terminator pairs; preprocessing the gene text to generate a raw genome sequence; searching the raw genome sequence for base sequences that fuzzy search matches the set of promoter-terminator pairs to generate a base sequence to be aligned; and comparing the base sequence to be aligned with base sequences in a bioinformatics database based on the BLAST gene alignment method to generate gene prediction and identification results. This invention, by separating base sequences from promoter-terminator pairs and then performing BLAST gene sequence alignment, achieves coarse gene localization followed by BLAST gene sequence alignment, providing a method for predicting new genes, improving alignment efficiency and comprehensiveness, as well as the accuracy and comprehensiveness of gene prediction results.

The present invention relates to a novel gene therapeutic agent capable of inhibiting FABP4 and / or FABP5, and relates to a nucleic acid construct capable of inhibiting target gene(s), FABP4 and / or FABP5, and use thereof for treating obesity or obesity-derived metabolic diseases. The nucleic acid construct according to the present invention effectively inhibits the expression of FABP4 and / or FABP5 by targeting adipocytes, thereby achieving the effects of reducing body weight, reducing inflammatory cytokines in adipose tissue, reducing lipids, and ameliorating insulin resistance and glucose tolerance, and thus can be used in the development of therapeutic agents for obesity or obesity-derived metabolic diseases.

A composition formulated for intrathecal delivery of a recombinant adeno-associated virus (rAAV) vector comprising an AAVhu68 capsid carrying a human galactosylceramidase (GALC) gene for administration to Krabbe patients is provided. Also provided are novel gene sequences and uses thereof. 20 26 20 50 62 29 J un 2 02 6 A B S T R A C T 2 0 2 6 2 0 5 0 6 2 2 9 J u n 2 0 2 6

This invention describes a novel gene regulatory sequence containing the promoter and enhancer sequences of the human cardiac troponin T gene (TNNT2) that selectively induces expression in cardiomyocytes. This novel TNNT2 promoter / enhancer composition can be used to induce adeno-associated virus gene expression, construct cell-type-specific expression vectors, or perform cardiac-specific transgenesis. The use of this novel promoter / enhancer composition is demonstrated by the expression of mAKAP shRNA and a mAKAP-derived anchor-disrupting peptide useful for the treatment of heart failure.

The present invention relates to methods for predicting the clinical outcome or the response of a patient suffering from cancer to an anti-cancer therapy based on novel gene signatures.

The invention discloses a rice chalky related gene Chalk9 and application thereof. The gene is any one of the following DNA molecules: 1) a DNA sequence as shown in SEQ ID NO.1; 2) polynucleotide for coding the protein sequence of SEQ ID NO.2; and 3) a DNA sequence which has more than 90% of homology with the DNA sequence as shown in SEQ ID NO.1 and is used for coding protein with the same function. A molecular marker v5 for identifying different alleles on a Chalk9 gene locus in rice is located at the position of a Chalk9 gene promoter-1331bp, and the nucleotide sequence of the molecular marker v5 is ATTATGCTTATAAGACAAAGCTTAAATTTTGAATTTTTTAATCTTTT. The molecular marker v5 can be used for identifying different alleles on the Chalk9 gene locus in rice, and the molecular marker v5 can be used for identifying different alleles on the Chalk9 gene locus in rice. The invention discloses a new application of a chalkiness control new gene Chalk9 in reducing chalkiness of rice, the gene has important application potential in cultivating high-yield and high-quality new varieties, and a new gene resource is provided for cultivating new high-yield and high-quality breakthrough crop new varieties.

The present disclosure identifies new genes which have the potential to increase broad acre yield in crops. This disclosure is based upon our fundamental knowledge of light signal transduction and our understanding of the roles these genes play in regulating plant growth and development in response to light. Transgenic plants with gain- or loss-of-function of one of these genes, or in combination, are expected to show significant improvements in broad acre yield and stress tolerance.