382 results about "Novel gene" patented technology

The invention discloses application of TaMYB-7B and a coding gene thereof in regulation and control of wheat tiller number, and belongs to the technical field of gene engineering. The invention finds for the first time that the TaMYB-7B gene is a novel gene capable of simultaneously regulating and controlling the tillering number and grain traits of wheat. The TaMYB-7B gene is interfered, knocked out, silenced or mutated, so that the tiller number of a plant can be increased, the grain length is increased, the hundred-grain weight is increased, finally, the yield of a single wheat plant can be increased, and a foundation is laid for wheat yield improvement and character improvement.

The invention belongs to the technical field of plant breeding and biology, and particularly relates to how to reduce the cadmium content of rice grains through a gene OsCT1 and application of the gene OsCT1 to creation of new germplasm of low-cadmium-accumulation rice. The invention discloses a nucleotide sequence of a gene OsCT1 coding region, a coded protein sequence, a CRISPR-Cas9 knockout target site nucleotide sequence, a nucleotide sequence of a knockout mutant and a protein sequence of the knockout mutant, as shown in an ST.26 standard sequence nucleotide or amino acid sequence table. After the gene OsCT1 is knocked out by utilizing a CRISPR-Cas9 technology, the cadmium content of grains of the OsCT1-knocked-out transgenic plant is obviously lower than that of a wild type variety. In addition, in the seedling stage, the plant height and the root length of the OsCT1-knocked-out transgenic plant are remarkably larger than those of a wild type variety; after the OsCT1 gene is subjected to overexpression, the grain cadmium content of an overexpressed transgenic plant is slightly higher than that of a wild type variety. In addition, in the seedling stage, the plant height and the root length of the overexpressed OsCT1 transgenic plant are obviously smaller than those of a wild type variety. According to the invention, the OsCT1 gene can be utilized to create a novel rice germplasm with low cadmium content in grains.

The invention discloses a rice salt stress resistant gene OsHAK11 coding protein and application thereof, and belongs to the field of plant genetic engineering. According to the method, a rice OsHAK11 gene (the nucleotide sequence is shown as SEQ ID NO.1) is knocked out through a CRISPR / Cas9 gene editing technology, and a mutant with significantly enhanced salt tolerance is obtained. The gene editing vector pEGCas9Pubi-B-OsHAK11 contains sgRNA of a region as shown in a target SEQ ID NO.4, a rice receptor material is transformed through agrobacterium tumefaciens mediation, and a plant with the OsHAK11 gene subjected to frame shift mutation is obtained through screening. A salt stress experiment shows that the survival rate of the mutant oshak11 is obviously higher than that of a wild type. The invention provides a new gene resource and an efficient technical means for salt-tolerant breeding of rice.

The invention discloses a method for screening and identifying a cucumber aphid-resistant gene as well as a screened gene and application thereof, and provides a brand-new method for screening and identifying the cucumber aphid-resistant gene, which is used for screening the cucumber aphid-resistant gene based on four omics technologies. The four types of omics comprise transcriptome, proteome, methylation group and metabolome before and after inoculation of aphids on cucumbers, and the cucumber aphid resistance regulation gene is screened through combined analysis, so that a new gene resource is provided for improving the aphid resistance of the cucumbers, and a new method and a new idea are provided for designing molecular genetic breeding. The CsPGD2 gene obtained through screening is an aphid resistance gene, the dynamic change of a metabolic substance 5-ribulose phosphate in a metabolome is consistent with that of a transcriptome gene CsPGD2, the aphid resistance function of the gene CsPGD2 is verified through VIGS, and the result proves that the gene CsPGD2 is an important regulation factor for the aphid resistance of cucumbers. The invention provides a new gene resource for improving the aphid resistance of cucumbers, and has a good application prospect.

The present disclosure relates to tobacco plants, plant parts, seeds, compositions and methods related to modulating expression of novel genes in tobacco to modulate alkaloid levels.

The invention belongs to the technical field of molecular biology breeding, and particularly relates to application of a GhCASPL1F1 gene in cultivation of high-temperature-resistant cotton germplasm, and an amino acid sequence of a protein coded by the GhCASPL1F1 gene is shown as SEQ ID NO.3. The invention also relates to application of the GhCASPL1F1 gene in cultivation of high-temperature-resistant cotton germplasm. The invention provides a novel gene GhCASPL1F1 for negative regulation of high-temperature resistance of cotton, and by negative regulation of expression of the gene, adaptability of plants to high temperature and high-temperature stress tolerance can be improved, male fertility in a high-temperature environment can be maintained and improved, and high-temperature resistance of the plants can be improved. The method has important theoretical value and practical significance for improving the high-temperature resistance of commercial crops and improving the yield and quality of the crops in a high-temperature frequent environment, has good economic benefits, and has wide application prospects in cultivation of new high-temperature-resistant varieties of cotton.

The invention discloses a gene RBOHB and application of the gene RBOHB in regulating and controlling waterlogging stress to induce cucumber adventitious root formation, a novel gene RBOHB for inducing cucumber adventitious root formation is identified, the adventitious root formation capability of an edited mutant of the gene RBOHB is weakened, the extension of the adventitious root is obviously inhibited, and the adventitious root formation capability of an overexpressed mutant is enhanced. The gene RBOHB disclosed by the invention is used for regulating and controlling the waterlogging tolerance of cucumbers, and has important application value in screening and improving waterlogging-tolerant germplasm resources of the cucumbers and breeding waterlogging-tolerant varieties of the cucumbers. The cucumber is subjected to transgenic genetic transformation through a gene editing technology and a gene overexpression technology, the cucumber waterlogging tolerance regulation gene RBOHB and application of the cucumber waterlogging tolerance regulation gene RBOHB in waterlogging tolerance cucumber varieties are provided, and the cucumber waterlogging tolerance regulation gene RBOHB has important application value in the field of breeding of resistant cucumbers.

The invention provides an application of a Brassica napus gene BnSKIP31 in prevention and control of sclerotiniose, which is an application in obtaining a disease-resistant enhanced rape material by creating a gene to edit rape. According to the invention, CRISPR gene knockout rape of BnSKIP31 is constructed, the negative regulation function of the gene on sclerotiniose resistance is disclosed for the first time, and application of the BnSKIP31 gene to acquisition of sclerotiniose rape materials with increased sclerotiniose resistance by creating CRISPR gene knockout rape is provided. The BnSKIP31 gene provided by the invention is a new gene resource suitable for creating and breeding new sclerotiniose-resistant rape materials and new varieties, and the created rape germplasm has lasting disease resistance.

The invention discloses an application of a multidrug-resistant efflux transporter gene OsMRET1 in regulation and control of chalkiness traits of rice. A nucleotide sequence of a coding region of the gene is shown as SEQ ID No.1, and a coded amino acid sequence is shown as SEQ ID No.2. The invention further discloses an application of the multidrug-resistant efflux transporter gene OsMRET1 in regulation and control of chalkiness traits of rice. It is found for the first time that the gene OsMRET1 can regulate and control formation of chalkiness, an OsMRET1 gene knockout mutant is created through a gene editing technology, an OsMRET1 gene overexpression strain is obtained through a transgenic technology, it is verified that the OsMRET1 gene is a new gene for regulating and controlling rice chalkiness, and overexpression of the gene can reduce rice chalkiness and improve rice chalkiness. And a new gene resource and a technical route are provided for rice quality improvement.

Genome sequencing identified a new gene associated with a cardiac arrhythmic disease, TAXI -binding protein 3. From cell line models and a conditional knock-out mouse models of the gene deletion, it was found that a specific molecule, transient receptor potential vanilloid 4 (TRPV4), is up-regulated and is associated with calcium mediated arrhythmic depolarizations.

The invention discloses application and a method of wheat demethylase TaALKBH23 in regulation and control of drought stress resistance of plants, a coding sequence of the demethylase TaALKBH23 is shown as SEQ ID NO: 1, and an amino acid sequence coded by the demethylase TaALKBH23 is shown as SEQ ID NO: 2. The invention provides demethylase TaALKBH23 and a function and application of a coding gene of the demethylase TaALKBH23 in regulation and control of plant drought resistance, and it is found through construction of a TaALKBH23 wheat genetic transformation material that the gene negatively regulates and controls wheat drought resistance. It is found that TaALKBH23 plays an important role in the process of regulating and controlling drought stress resistance of plants, and a new gene resource is provided for improving drought resistance of crops.

Provided is a gene ZmFIE2 for regulating maize kernel development, an encoded protein thereof, an InDel molecular marker, and a use thereof, aiming to solve the current technical problems of low maize haploid induction rate, long breeding cycle, and low maize yield. An InDel molecular marker for detecting ZmFIE2 mutants was screened out, and a primer for InDel molecular marker was designed, the InDel molecular marker and the primer thereof can be applied to the breeding of maize kernel size varieties and maize haploid or diploid lines. From the epigenetic level, it was found that ZmFIE2 regulates the development of maize embryo and endosperm, affects the accumulation of kernel storage materials, and affects the cell cycle process of endosperm cells, which has great value in improving maize yield. By using multiple created ZmFIE2 allele mutants, research has found that the loss of ZmFIE2 function can directly determine the division of endosperm cells and apomixis, which will provide new gene resources and new ways to achieve high-frequency haploid induction and heterosis fixation in maize.

The invention provides an application of a phosphatidylinositol monophosphate 5-kinase gene BnPIP5K9 of rape in regulating and controlling the oil content of the rape. The genetic basis of the oil content of rape seeds is analyzed on the basis of multiple omics, and it is found that the expression quantity of the phosphatidylinositol monophosphate 5-kinase gene BnPIP5K9 in rape is significantly positively correlated with the oil content; the gene BnPIP5K9 is subjected to gene overexpression and knockout to create a mutant for functional verification by further utilizing a tobacco mosaic virus double-35S promoter and a CRISPR / Cas9 gene editing technology, the result shows that the oil content of rape seeds can be remarkably increased by 2.16-2.77% by overexpression of the gene, in addition, rape germplasm resources with gene BnPIP5K9 function deficiency can be obtained through the CRISPR / Cas9 technology, and the rape seed quality is improved. A new theory and a new gene resource are provided for high-oil breeding and oil improvement of the rape, and the method has extremely high application value and potential.

The invention relates to the field of biological medicine and aquatic products, and discloses a grass carp IFNf interferon and truncated protein and application thereof in virus resistance or bacterium resistance. The applicant discovers the new subtype IFNf of the grass carp interferon for the first time, the protein coded by the new gene has an efficient antiviral effect, and the derived peptide AMP-G2 of the grass carp interferon has broad-spectrum antibacterial activity. The sensitivity of the grass carp interferon IFNf to grass carp reovirus (GCRV) infection is higher than that of other interferon, and the grass carp interferon IFNf shows obvious antiviral activity on the cellular level and can be used for preparing antiviral drugs. The interferon-derived peptide provided by the invention is a host natural peptide, is small in molecular weight and simple to synthesize, has a remarkable bactericidal effect on various bacteria and fungi, and can be used for preparing broad-spectrum antibacterial agents or antibacterial drugs.

The invention discloses a sugarcane ScPP2C49 gene and application thereof, and relates to the technical field of plant biology, the nucleotide sequence of the ScPP2C49 gene is as shown in SEQ ID No.1, and the amino acid sequence of protein coded by the ScPP2C49 gene is as shown in SEQ ID No.2. The gene is induced to express by drought and abscisic acid, and the encoded protein of the gene is positioned in a cell nucleus. Functional studies show that after overexpression of the ScPP2C49 gene in the plant, the drought resistance of the plant is negatively regulated through various mechanisms of inhibiting an abscisic acid signal channel, hindering stomatal closure under drought stress, weakening active oxygen scavenging ability, reducing photosynthetic efficiency, inhibiting root growth and the like. The invention also provides a recombinant overexpression vector containing the gene and a method for obtaining a transgenic plant with reduced drought resistance by using the vector through an agrobacterium-mediated method. According to the invention, a new gene resource is provided for deep analysis of a plant drought-resistant molecular mechanism, and an important target gene is provided for cultivation of high-stress-resistance crop varieties through a reverse genetics means (such as gene knockout).

The invention discloses an application of an OsPAHX5 gene and a protein coded by the OsPAHX5 gene in regulation and control of rice grain length, and belongs to the technical field of plant breeding and biology. The invention discloses a nucleotide sequence of a coding region of an OsPAHX5 gene, a coded amino acid sequence, a nucleotide sequence of a CRISPR-Cas9 knockout target site, a nucleotide sequence of a knockout mutant and an amino acid sequence of the knockout mutant, which are shown in an ST.26 standard sequence nucleotide or amino acid sequence table. The function of the OsPAHX5 gene is researched by utilizing molecular biology and a plant transgenic technology, and it is found that after the OsPAHX5 gene is knocked out by utilizing a CRISPR-Cas9 technology, the grain length of a knocked-out transgenic plant is remarkably larger than that of a wild type variety; after the OsPAHX5 gene is subjected to overexpression by using a transgenic overexpression technology, the grain length of an overexpressed transgenic plant is not obviously different from that of a wild type variety. According to the invention, the OsPAHX5 gene can be utilized to create a rice variety with larger grain length.

The invention discloses a novel gene editing carrier system and application thereof in cultivation of beany-flavor-free soybeans, and belongs to the technical field of gene engineering. According to the invention, by constructing a single transcript pUBQ3: Bar-P2A-DsRED2, the efficient knockout of five key genes, namely GmLOX1, GmLOX2, GmLOX3, GmFAD2-1A and GmFAD2-1B, in the soybean is successfully realized, the single transcript pUBQ3: Bar-P2A-DsRED2 adopts a soybean endogenous strong promoter pGmUBQ3 which is connected in series with a resistance gene Bar and a fluorescent gene expression cassette DsRED2, and the Bar is separated from the DsRED2 through P2A. The method can significantly reduce the beany flavor of soybeans, and has good application prospects in soybean quality improvement and breeding.

Provided herein are methods of expressing syntaxin-binding protein 1 (STXBP1) in a neuron. Also provided herein is a modified adeno-associated virus (AAV) encoding a STXBP1 under the control of a promoter operable in a brain cell or neuronal cell.

The invention provides a gene function prediction method and system based on multi-label active learning, and the method comprises the steps: selecting gene-function pairs forming an initial set from an unlabeled data set according to an initial information score quantified by the uncertainty of gene function prediction, label correlation and label distribution imbalance; according to a selection standard of feature diversity, label space diversity and sample-label pair interaction diversity, a standby set is selected in combination with the initial information score; the gene function prediction efficiency and accuracy are remarkably improved, reliable technical support is provided for large-scale gene function research, and the method is particularly suitable for complex function prediction scenes such as research fields of new gene function discovery, disease-related gene function annotation and the like and has important practical application value.

The present invention discloses the application of TMPRSS9 gene in the diagnosis and treatment of venous thromboembolism, and belongs to the field of medical diagnosis and treatment technology. The present invention verifies the association between missense mutations of TMPRSS9 gene and thrombophilia in human population, in vivo and in vitro, and draws the following conclusions: nonsense mutations and missense mutations at the sites of c.334C>T, c.692C>T, c.917G>A, and c.1709C>T of TMPRSS9 gene are representative in the effect of TMPRSS9 gene mutation on the occurrence of venous thrombosis. The discovery of the above-mentioned pathogenic gene mutations can prevent and treat thrombophilia at an early stage, which is of great significance for the diagnosis, treatment and management of thrombophilia. At the same time, the inclusion of the new TMPRSS9 gene enriches the genetic variation spectrum of venous thromboembolic diseases, which is of guiding significance for the screening of diseases.

Provided are plants, cells, tissues, and germplasm thereof comprising genes and marker alleles associated with increased resistance to gray leaf spot disease. Also provided are breeding methods and methods of identifying and selecting plants having alleles associated with increased resistance to gray leaf spot. Provided are methods to identify novel genes that encode proteins providing plant resistance to gray leaf spot and uses thereof. The disclosed genes and marker alleles are useful in the production of gray leaf spot disease resistant plants through breeding, transgenic modification, or genome editing.

The present invention provides novel gene therapy compositions for use in treatment of Duchenne Muscular Dystrophy (DMD).

Provided are plants, cells, tissues, and germplasm thereof comprising genes and marker alleles associated with increased resistance to gray leaf spot disease. Also provided are breeding methods and methods of identifying and selecting plants having alleles associated with increased resistance to gray leaf spot. Provided are methods to identify novel genes that encode proteins providing plant resistance to gray leaf spot and uses thereof. The disclosed genes and marker alleles are useful in the production of gray leaf spot disease resistant plants through breeding, transgenic modification, or genome editing.

To provide a method for identifying a gene encoding an apiose transferase for converting apigenin into apiin and a gene encoding a glucose transferase, and consistently producing apiin from apigenin by using the apiose transferase and the glucose transferase encoded by the gene.SOLUTION: Provided is an apiose transferase including a specific amino acid sequence. Further, provided is a method for producing apiin from apigenin 7-O glucoside which includes a step of bringing the apiose transferase, apigenin 7-O glucoside and UDP-apiose in contact each other.SELECTED DRAWING: None

The invention belongs to the technical field of autoimmune skin disease immunotherapy, and provides a three-target novel chimeric autoantibody receptor structure which comprises three chimeric receptors through two connecting peptides, specifically, a first chimeric autoantibody receptor comprises a Dsg1 extracellular structural domain; the second chimeric autoantibody receptor comprises a Dsg3 extracellular structural domain; the third chimeric antigen receptor comprises an extracellular domain capable of targeting a B cell associated antigen; wherein the Dsg1 extracellular structural domain is an extracellular structural domain of desmosome core glycoprotein 1; the Dsg3 extracellular structural domain is the extracellular structural domain of desmosome core glycoprotein 3. In-vitro experiments and animal experiments verify that the novel three-target CAAR-T cells can significantly increase the release of cytokines, enhance the targeted killing activity of the cytokines, and show better multiplication capacity; a novel cellular immunotherapy technology is provided for treating autoimmune skin diseases, and the application of CAAR-T in the field of autoimmune disease treatment can be expanded.

The invention discloses a novel gene OsWRKY24 influencing cold resistance of rice and an identification method thereof. The novel gene OsWRKY24 is prepared by comparative transcriptome research screening carried out by near-isogenic lines Towada and ZL31 in a rice seedling stage under cold stress. A rice seedling stage cold tolerance related gene OsWRKY24 is screened by utilizing a transcriptome re-sequencing method, the function of the gene is verified by constructing a transgenic knockout material, and meanwhile, a gene sequence, an amino acid sequence, a promoter sequence, expression characteristics and a phylogenetic tree are analyzed; theoretical basis and gene resources are provided for cold-resistant improvement and breeding of rice; a novel rice cold sensitive gene OsWRKY24 is identified, and the cold tolerance of rice can be improved by gene mutation.

The invention provides a function and application of a transcription factor NAC42. The invention discloses a novel gene NAC42 which is closely related to regulation and control of phytoalexin, volatile monoterpenes and sesquiterpenes of plants (so as to regulate and control insect resistance). The invention provides a new way for improving the insect resistance of plants.

In order to control plant diseases caused by Xanthomonas bacteria, a novel bacteriophage exhibiting bacteriolytic activity specific to the same bacteria is isolated, and a plant disease control composition containing the same as an active ingredient is developed and provided. Provided are a lysozyme containing a bacteriophage having a novel genomic DNA sequence and exhibiting a lysolytic activity specific to Xanthomonas bacteria, and a plant disease control composition containing the lysozyme as an active ingredient.

The application discloses application of a rice disease resistance related gene Os04g55720 in bacterial blight breeding and relates to the technical field of modern biotechnology and genetic engineering technology.The rice disease resistance related gene Os04g55720 has a nucleotide sequence as shown in SEQ ID NO.1, is composed of 2821 nucleotides, has a coding region sequence as shown in SEQ ID NO.2, and has a sequence of 2148 nucleotides; the gene encodes a protein with 613 amino acids, and has an amino acid sequence as shown in SEQ ID NO.3.The application proves that overexpression of Os04g55720 enhances the resistance of rice to bacterial blight by means of molecular biology and biochemical technology, and shows that the rice protein OsPGDH-3 can effectively control the incidence of rice bacterial blight at a relatively low level.The application provides a new idea, strategy and gene resource for green prevention and control of rice bacterial blight.

The application discloses application of a corn gene in improving corn kernel protein content, and relates to the technical field of biology. ZmAAP16 A new gene capable of improving corn kernel content is mined by the application. ZmAAP16 Through transgenic method, function of the gene is analyzed and identified in depth, it is found that the gene can significantly improve the corn kernel protein content by overexpression in plants, and the role of the gene in improving the corn kernel protein content is determined. The application has important significance for cultivation of high-protein ensiled corn.