63 results about "Tissue specific" patented technology

The present invention relates to a novel lipid compound for tissue-specific delivery, and a lipid nano-particle (LNP) composition comprising the same, the lipid nano-particle comprising a modified lipid compound as a component, according to the present invention, internal active substances are selectively delivered into cells of specific tissues such as lymph nodes, spleen, retina, cancer, brain, liver and the like in vivo, thereby preventing side effects and safely exhibiting a desired level of effect. The tissue-specific non-viral LNP delivery vectors can be effectively used for prevention of infectious diseases and treatment of rare and refractory (hereditary) diseases (diseases which are effectively and selectively delivered to in-vivo targeted sites, such as macular degeneration, diabetic retinal degeneration, hereditary retinal degeneration, cancer, cerebral diseases, liver diseases and the like).

The invention discloses a recombinant adeno-associated virus vector for targeted delivery of VEGF-C (vascular endothelial growth factor-C) and application of the recombinant adeno-associated virus vector in promoting endocardial differentiation to treat coronary heart disease. The rAAV vector comprises transgene for coding VEGF-C protein or VEGF-C protein functional variant and a tissue-specific promoter operably connected to the transgene, wherein the tissue specific promoter is capable of driving the transgene to be specifically expressed in the heart endothelial cells. According to the treatment method developed by the invention, specific expression of the Vegfc gene in the heart is driven by using an ICAM2 promoter with targeting to endothelial cells, and a remarkable curative effect is shown in a myocardial infarction model mouse. The therapeutic scheme provides a new effective strategy for intervention and treatment of myocardial infarction and chronic heart failure, and has the advantages of high safety, long gene expression duration, extremely low immune response to a host and the like.

Particulated and reconstituted tissues comprising small, densely packed tissue microparticles encapsulated in a tissue specific promoting gel packed at a percolation threshold that can be transplanted into damaged tissue thereby facilitating regeneration following trauma to the tissue. The engineered microparticle construct for tissue replacement and repair, as taught herein, provides numerous benefits including (1) encouraging a regenerative response in damaged tissue regions, (2) mimicking the structural support of native tissue, (3) establishing an environment that promotes attachment, migration, and differentiation of infiltrating stem cells, and (4) providing a source of growth factors and other anti-catabolic growth factors and cytokines. Tissue specific microparticles packed together at, or past, their percolation threshold will provide the necessary mechanical environment and to best recapitulate and integrate with native tissue. The packing of microparticles, derived from the ECM of native tissue to a concentration past the percolation point will yield both the necessary biochemical and biomechanical properties necessary for reconstituting a specific tissue.

The present invention relates to: an AAV-based PDE6B viral vector for treating retinitis pigmentosa, the AAV-based PDE6B viral vector containing a tissue-specifically expressed PDE6A promoter; and a use of thereof, and provides a gene therapy for treating retinitis pigmentosa caused by PDE6B gene deficiency. The in vivo therapeutic efficacy of seven types of AAV5-PDE6B vectors was confirmed using an AAV by using a PDE6A promoter that is tissue-specifically expressed in photoreceptor rod cells that develop retinitis pigmentosa. An AAV5-PDE6A-450-PDE6B vector was selected as a candidate due to exhibiting strong tissue-specific expression in photoreceptor rod cells under even off-target conditions, unlike the gene expression characteristics of an AAV5-CMV-PDE6B vector, and was tested so as to be usable in the development of a gene therapeutic agent for treating PDE6B-deficient retinitis pigmentosa patients. Therefore, the present invention, related to AAV5-PDE6B for retinitis pigmentosa treatment and containing a tissue-specifically expressed PDE6A promoter, provides retinitis pigmentosa patients with an important treatment option having improved safety, and can be expected to have fundamental therapeutic effects compared to conventional treatments.

The present invention is directed to a personalized in vitro immune response simulation system, including: a plurality of containers, each container including a culture of tissue-specific cells and endothelial cells in fluid contact with the tissue-specific cells; a liquid medium comprising at least about 25% serum or serum replacement (SR); immune cells suspended in the liquid medium; and a linkage system establishing fluidic coupling between the plurality of containers, allowing the liquid medium to flow through the linkage system, wherein the system is an isogenic system. The present invention further provides methods for preparation of such a system, and for uses thereof.

The invention relates to the technical field of medical image processing, and discloses a cardiovascular plaque component unmarked recognition system and method, and the system comprises a data collection unit, a time domain and frequency domain combined feature extraction algorithm module, a data processing unit, a data transmission unit, a three-dimensional imaging unit and a background processing unit. The time domain and frequency domain combined feature extraction algorithm module maps original measurement pulse sound pressure data to a Hilbert function space to form multi-dimensional feature representation, a tissue specificity functional constraint model is constructed, and multi-scale feature integration is achieved through geodesic distance calculation; the data processing unit generates three kinds of tissue simulation data based on a dual-wavelength optical absorption spectrum principle; the three-dimensional imaging unit obtains different phase data in a two-dimensional rotation acquisition mode, a three-dimensional image is generated through a multi-wavelength optical component decomposition imaging algorithm, a contrast agent or a marker does not need to be used, and accurate distinguishing of a lipid core, a calcification area and a basic tissue is achieved.

The present invention provides constructs comprising a transgene operably linked to a novel tissue-specific maize promoter selected from ris2, Ihcb10, nas2, aa2m, and expb14. Methods of using the constructs to drive tissue-specific transgene expression in a maize plant are provided. Maize plants comprising the constructs or made by the methods are also provided.

Described herein are recombinant viral genomes comprising a nucleotide sequence encoding a telomerase reverse transcriptase (TERT) operably linked to a tissue-specific and / or organ-specific promoter. Aspects described herein may be used in the treatment of conditions associated with shortened telomere length, such as pulmonary fibrosis, myocardial infarction and conditions associated therewith.

Tissue-specific progenitor cells representing the endoderm, ectoderm, and mesoderm lineages are generated from pluripotent sources. Progenitor cell generation is accomplished by contacting pluripotent stem cells with tissue-specific exosomes, microRNAs, proteins, and peptides, obtained by stressing the tissue under in vitro conditions. In vitro-generated tissue organoids are used as a source of "differentiation factors" for generating individualized progenitor cells. For example, to generate lung progenitor cells, allogeneic stem cells are implanted into decellularized cadaveric lung tissue to form lung structures, which are then exposed to various cellular stressors and conditioned media to differentiate the pluripotent stem cells into progenitor cells. Extracellular matrix can be obtained from decellularized structures seeded with regenerating cells and used to generate organoids, which are then subjected to stress to produce differentiation factors.

The present disclosure relates to a method of isolating extracellular vesicles directly from human tissues. The invention further relates to a method of identifying disease and tissue specific membrane proteins on extracellular vesicles by membrane isolation and proteomic analysis. The invention further relates to methods of diagnosing diseases by capturing extracellular vesicles by the use of disease specific membrane proteins from body fluids, and detecting or analyzing molecular signatures (proteome, DNA, and RNA) on captured extracellular vesicles. Moreover, the present invention relates to kits, apparatus and software required for implementing aforementioned methods.

The present disclosure provides, among other things, methods and compositions in which a nucleic acid sequence includes a mammalian scaffold matrix attachment region (S / MAR) operably linked with a coding sequence. The present disclosure provides S / MARs useful in expression vectors (e.g., for use in gene therapy approaches) to achieve a particular, improved, or target level, duration, and / or tissue specificity of expression of an operably linked coding sequence.

The present application relates to the technical field of health care informatics, in particular to a system for predicting depression of teenagers, comprising an acoustic characteristic representation module, including a co-integrated ultrasonic transducer array and electroencephalogram electrode, an electrode substrate embedded with an acoustic impedance sensor, and a temperature-sensitive acoustic coupling adhesive layer on the surface of the electrode substrate; a multi-layer medium acoustic modeling module: used for establishing an accurate head tissue acoustic transmission model according to individual anatomical structure characteristics, for quantifying the frequency dispersion effect generated when the electroencephalogram signal propagates in different tissue layers, etc.; solving the problem that in the prior art, due to the change of the myelin sheath thickness of teenagers, the tissue-specific dispersion has a systematic influence on the functional connection network, resulting in that the depression-related network abnormalities and the development-related transmission changes cannot be distinguished.

Provided are a tissue tracing method for cell-free DNA, a tissue lesion risk notification method, a cell-free DNA-based cancer risk indication method, a cell-free DNA-based gestational disease risk indication method, a cell-free DNA-based receptor tolerance risk notification method, a disease treatment effect evaluation or prognosis method and apparatus, an electronic device, a computer-readable storage medium, a computer program product and a computer program. The tissue tracing method for cell-free DNA comprises: acquiring a tissue-specific expression gene set of a tissue; acquiring first distribution information of cell-free DNA derived from a sample on specific regions of genes in the tissue-specific expression gene set; and, on the basis of the first distribution information, acquiring a tissue contribution index of the tissue in the cell-free DNA, so as to trace the tissue of the cell-free DNA, wherein the specific regions comprise transcription start site (TSS) regions.

The invention relates to the technical field of medical care informatics, in particular to a teenager depression prediction system. Comprising an acoustic characteristic characterization module, the acoustic characteristic characterization module comprises an ultrasonic transducer array and electroencephalogram electrode co-integration, an acoustic impedance sensor is embedded in an electrode substrate, and a temperature-sensitive acoustic coupling adhesive layer is arranged on the surface of the electrode substrate; the multi-layer medium acoustic modeling module is used for establishing an accurate skull tissue acoustic transmission model according to the characteristics of an individual anatomical structure, and is used for quantifying the frequency dispersion effect and the like generated when the electroencephalogram signals are transmitted in different tissue layers; the problem that depression-related network abnormity and development-related transmission change cannot be distinguished due to systematic influence of tissue-specific dispersion on a functional connection network due to teenager myelin sheath thickness change in the prior art is solved.

A method of identifying a biomarker present in a defined category of tissue in an organism at an elevated level when compared to a level in at least one different category of tissue, wherein the biomarker is detectable on extracellular vesicles secreted from cells of the defined category of tissue. The method comprises the steps of: (i) identifying a candidate biomarker; (ii) providing a binding agent capable of specifically binding to the candidate biomarker identified in step i. (iii) detecting the presence of a candidate biomarker on extracellular vesicles obtained from a sample of defined categories of tissue by binding a binding agent to the candidate biomarker in the sample, where the candidate biomarker is not present on the extracellular vesicles obtained from the sample of defined categories of tissue, compared to the extracellular vesicles from at least one different category of tissue of the organism, extracellular vesicles from defined categories of tissue preferentially bind to a binding agent; and (iv) selecting the candidate biomarker as a biomarker present at an elevated level in the defined category of tissue. Also provided are methods of enriching a biological sample comprising a mixture of extracellular vesicles to generate an enriched fraction of extracellular vesicles from defined categories of tissue. Also provided are methods of analyzing the contents of extracellular vesicles in an enriched fraction of extracellular vesicles from defined categories of tissue.

The invention discloses a TabNet-based interpretable multi-tissue DNA methylation age prediction method and system, and relates to the technical field of bioinformatics and computer crossing, and the method comprises the following steps: obtaining DNA methylation data of a to-be-detected sample, and preprocessing the data; a methylation beta value of a feature set composed of 2035 preset CpG sites is extracted from the preprocessed data to serve as an input feature; the input features are loaded to an age prediction model based on a TabNet framework obtained through a specific training process for calculation, the predicted DNA methylation age of the to-be-detected sample is output, and through screening of a simple preset CpG site with multi-tissue stability, tissue specific signals are accurately captured in cooperation with a TabNet dynamic attention mechanism, so that the DNA methylation age of the to-be-detected sample is predicted. The black box dilemma of other deep learning models is effectively solved, and meanwhile, the technical problems of insufficient prediction precision, weak generalization ability in extreme age groups, poor robustness in practical application and the like of a traditional machine learning model are solved.

The invention relates to the technical field of biomedical detection, and discloses a system for accurately detecting background signal subtraction of free RNA (Ribonucleic Acid) for early diagnosis of lung cancer. Comprising a sample collection module used for obtaining whole blood of a detected sample; a base signal determination module for determining a base signal level based on the whole blood; the measured value recording module is used for recording the measured value of the differentiated cancer tissue specific RNA transcript in the plasma; the machine learning subtraction module is used for carrying out signal subtraction processing on the basic signal level and the measured value through a random forest machine learning method; and the artificial selection module is used for carrying out qualitative selection on the cancerous genome region after the signal deduction processing, and realizing background signal deduction of free RNA in the non-small cell lung cancer pathological sample and accurate verification of the cancerous genome region in combination with a selection result corresponding to the qualitative selection so as to assist in early diagnosis of lung cancer. Background signals are efficiently reduced, and tissue-specific RNA targets are accurately screened.

The invention discloses a pancreatic tissue specific expression recombinant adeno-associated virus vector and application thereof, and belongs to the technical field of biological medicine. Comprising the following steps: S1, cloning a pancreatic specific promoter: jointly using Ins2 and Pdx1 promoters to prepare a Pdx-1 / Ins2 promoter; performing serotype and capsid mutation optimization; s2, insertion of an exogenous gene expression cassette: cloning a target gene to the downstream of a Pdx-1 / Ins2 promoter to construct a'promoter-exogenous gene-polyadenylic acid signal 'expression unit; s3, regulation and control element integration: inserting an HS4 insulator sequence into two sides of an expression box, and adopting Gibson assembly to realize seamless connection; constructing an SIN structure: deleting a U5 region in ITR through site-specific mutagenesis or enzyme digestion, and verifying that no residual promoter activity exists; s4, virus packaging and purification: packaging rAAV particles by adopting an HEK293 cell three-plasmid co-transfection system; the three plasmids comprise a carrier plasmid, an auxiliary plasmid and a packaging plasmid; and determining the purity after purification.

The present application relates to the technical field of extracellular vesicle sorting, and particularly relates to a epididymal body specific sorting marker, and an epididymal body sorting kit and method. The present application finds that CD52 can be used as a specific marker for sorting epididymal bodies. The present application is the first time to directly separate epididymal bodies from human seminal plasma, the sample is easy to obtain, and the operation process is non-invasive, safe, and the separation path is simple and easy to operate. In addition, it provides a method for the separation of other tissue-specific EV subpopulations. The present application can contribute to further explore the effect of epididymal bodies on sperm function and improve sperm function, and epididymal bodies as a marker have great potential for clinical diagnosis and treatment.

This invention discloses a controllable gene expression system based on homology-directed repair and its application, belonging to the field of gene therapy technology. The system is delivered via separate first and second vectors and includes: a targeting unit containing homologous arm sequences flanking a target gene locus exhibiting pathological state-dependent expression characteristics; an editing unit containing a CRISPR / Cas gene editing tool; and an effector unit containing a repair template composed of a foreign coding gene held by the homologous arm sequences. After the editing unit breaks at the target gene locus, the repair template integrates into the genome through homology-directed repair, allowing the expression of the foreign gene to be controlled by the regulatory elements of the endogenous target gene. This invention achieves intelligent dynamic regulation of the specific activation of exogenous therapeutic genes under pathological conditions and silencing them under normal conditions. Furthermore, through the division of labor between the two vectors and the synergy with tissue-specific promoters, it greatly improves the accuracy and safety of gene editing.

This invention relates to a method and system for colorectal cancer MRI image segmentation based on multi-dimensional feature fusion, belonging to the field of medical image processing technology. The invention first collects multi-source data from colorectal cancer patients and preprocesses the data, constructing a multi-scale feature extraction module to obtain image feature data. Then, through a multi-dimensional feature fusion module, it integrates seven dimensions of features: texture, shape, grayscale, modal correlation, lifestyle, functional metabolism, and tissue specificity, combining a spatial-channel attention mechanism to achieve feature selection and weight allocation. Finally, it decodes features using a U-Net network and optimizes the segmentation boundary using a fully connected conditional random field (CRF) with an adaptive potential function. This invention improves the accuracy and robustness of colorectal cancer MRI image segmentation through multi-modal data integration, multi-scale feature extraction, multi-dimensional feature fusion, attention mechanism empowerment, and the combination of U-Net network decoding and CRF post-processing.

The invention belongs to the technical field of biomedical hydrogel, and particularly relates to a wet adhesion tissue specific hydrogel patch and a preparation method thereof. The wet adhesion tissue specific hydrogel patch is prepared from methacrylated silk fibroin, a methacrylated acellular matrix, phenyl (2, 4, 6-trimethylbenzoyl) lithium phosphate and tannic acid, and the wet adhesion tissue specific hydrogel patch is prepared from the methacrylated silk fibroin, the methacrylated acellular matrix, the phenyl (2, 4, 6-trimethylbenzoyl) lithium phosphate and the tannic acid. The preparation method mainly comprises the steps of hydrogel solution preparation, illumination crosslinking and tannic acid soaking. The preparation method can be used for flexibly preparing a double-sided or single-sided adhesive patch. The hydrogel patch disclosed by the invention has excellent wet adhesion performance, tissue specificity, biocompatibility and mechanical property, and is remarkable in hemostatic effect; the hydrogel patch can avoid secondary injury and foreign matter inflammation risks caused by traditional suture, realizes rapid closing of wet wound surfaces, efficient hemostasis and tissue repair, is suitable for scenes such as surgical operations and wound first aid, and has important clinical application value.

The present disclosure relates to a tissue-specific promoter system for expressing microRNA (miRNA) for RNA interference-based methods of gene therapy. In these systems, the miRNA will inhibit gene expression or replace natural miRNA expression using microRNA.

Provided are an artificial intelligence-based early cancer diagnosis method and device using a method of inputting information on a cell-free DNA distribution of a tissue-specific regulatory region to an artificial intelligence model learned to early diagnose cancer and analyzing the information, and an information providing device and a storage medium.SOLUTION: A method for providing information for early cancer diagnosis based on artificial intelligence includes extracting a nucleic acid from a biological sample to obtain sequence information, arranging the obtained sequence information in a reference chromosome sequence database, selecting a nucleic acid fragment of a regulatory region based on the arranged sequence information, generating the selected nucleic acid fragment as image data, and inputting the generated image data to an artificial intelligence model learned to distinguish a normal image and a cancer image, analyzing the image data, and comparing the image data with a reference value to determine the presence or absence of cancer.SELECTED DRAWING: Figure 1

Provided in the present invention is a tissue-specific RcSDH2-3 gene promoter from Ricinus communis, which can drive gene expression in seeds and cotyledon-stage seedlings of plants, or in roots, stems, leaves, flowers, seeds and cotyledon-stage seedlings of plants.

Abstract Antibody drug conjugates (ADC’s) that bind to 191P4D12 protein and variants thereof are described herein. 191P4D12 exhibits tissue specific expression in normal adult tissue, and is aberrantly expressed in the cancers listed in Table I. Consequently, the ADC’s of the invention provide a therapeutic composition for the treatment of cancer. Abstract 20 26 20 50 95 30 J un 2 02 6 A b s t r a c t 2 0 2 6 2 0 2 6 2 0 5 0 9 5 3 0 J u n

By means of co-expression interference on expression of retinoic acid-induced gene I (RIG-1), virus transfection in transfection cells and expression of recombinant virus payload are enhanced. In some embodiments, the co-expression is driven by a tissue-specific promoter.

Provided are nucleic acids comprising an engineered tissue-specific exon / intron cassette that comprise a first nucleotide sequence comprising at least one exon specifically expressed in a dorsal root ganglion (DRG) neuron, optionally comprising an engineered start codon, an engineered non-sense mutation or an engineered an out-of-frame indel, and optionally comprising a second nucleotide sequence, wherein the first sequence and the second sequence are operably linked to a promoter and / or optionally other regulatory elements such that the first and second sequences are expressed in a cell comprising the nucleic acid; vectors comprising the nucleic acids; cells comprising the nucleic acids; and methods for their use in modulating the expression of the nucleic acid of interest.

This invention discloses a tissue-specific promoter for the LjC3H1 gene in honeysuckle, relating to the field of biotechnology. The nucleotide sequence of the specific promoter is shown in SEQ ID NO:1. This invention also provides an expression cassette containing the LjC3H1 gene tissue-specific promoter, a recombinant vector, and a host cell. It has the function of driving or regulating the expression of the LjC3H1 gene to further increase chlorogenic acid production.