67 results about "Gene conversion" patented technology

The invention discloses an application of a TaAP2-4 gene in regulating and controlling starch content of wheat grains, which is used for enhancing the expression quantity of the TaAP2-4 gene to improve the starch content of the wheat grains and weakening the expression quantity of the TaAP2-4 gene to reduce the starch content of the wheat grains. The TaAP2-4 gene is located on a chromosome 4 of a wheat genome, and three copies, namely TaAP2-4-A, TaAP2-4-B and TaAP2-4-D, are formed on the chromosome 4. According to the invention, a Crispr / Cas9 gene editing vector for knocking out the TaAP2-4 gene is constructed by adopting a reverse genetics method, the TaAP2-4 gene is successfully knocked out in a wheat material by adopting an agrobacterium-mediated method, and the starch content of a knocked-out mutant is reduced, so that the condition that the TaAP2-4 gene plays a positive regulation role in a wheat grain starch synthesis process is known. In practical application, directional knockout mutation of the genome level TaAP2-4 gene can be achieved through a gene transformation process, the gene knockout mutation method can be used for verifying functions of the TaAP2-4 gene, and materials are provided for mining other starch synthesis related genes.

This invention discloses the application of the wheat gene TaPSS2 in improving plant drought resistance, belonging to the field of functional genomics. The nucleotide sequence of the wheat gene TaPSS2 is shown in SEQ ID NO: 2. Transforming this gene into the model plant Arabidopsis thaliana can significantly improve the drought resistance of Arabidopsis. If the wheat gene TaPSS2 is transformed into wheat, rice, maize, cauliflower, and other plants, it is possible to obtain new germplasm with drought-resistant characteristics, which is of great significance for the breeding of superior crop varieties and their widespread application in production.

The invention belongs to the technical field of biology, and relates to application of a ZmCASPL11 gene in improving heat resistance of plants. The ZmCASPL11 gene encodes a protein of a membrane protein (CASPL) family related to a Kjeldahl band structure, the gene expression level of the ZmCASPL11 gene under high-temperature stress is remarkably increased, and a mutant zmcaspl11 of the gene shows a high-temperature sensitive phenotype. The ZmCASPL11 gene disclosed by the invention is transformed into arabidopsis thaliana and is subjected to functional verification, and compared with wild type arabidopsis thaliana, the tolerance of an arabidopsis thaliana plant over-expressed with the ZmCASPL11 gene to heat stress is remarkably enhanced. According to the invention, more theoretical bases are provided for corn heat resistance research, and gene resources and biotechnological means are provided for subsequent cultivation and germplasm resource innovation of new heat-resistant corn strains.

This invention discloses the application of the SPL23 gene in regulating the number of tassel branches in maize, including: applying a miR529-insensitive form of the SPL23 gene to regulate the number of tassel branches in maize. The method for increasing the number of tassel branches in maize is characterized by: constructing a vector containing a miR529-insensitive form of the SPL23 gene; and transforming the constructed miR529-insensitive form of the SPL23 gene into maize to obtain maize plants with an increased number of tassel branches. This invention, by constructing miR529-insensitive SPL23 plants, enables the SPL23 gene to resist miR529 degradation and thus undergo overexpression, significantly promoting tassel development and increasing the number of tassel branches, providing an effective approach for the genetic improvement of maize varieties with fewer male tassels.

Provided herein is a transgenic chicken comprising a genome having an endogenous immunoglobulin heavy chain gene in which the IgY CH1 coding sequence is functionally deleted. In certain embodiments, in B cells of the chicken the endogenous immunoglobulin heavy chain gene comprises: (a) a functional immunoglobulin heavy chain gene comprising, in operable linkage, a nucleic acid encoding an antibody heavy chain variable domain and a constant region coding sequence in which the IgY CH1 coding sequence is functionally deleted; and (b) a plurality of pseudogenes that are operably linked to said functional immunoglobulin heavy chain gene and that donate, by gene conversion, nucleotide sequence to the nucleic acid encoding the variable domain of (a), wherein the pseudogenes are upstream of the nucleic acid encoding an antibody heavy chain variable domain of (a).

This disclosure provides, among other things, a transgenic animal that uses gene conversion for antibody diversification, comprising B cells in which the endogenous immunoglobulin heavy chain locus comprises: (a) a functional immunoglobulin heavy chain gene comprising a nucleic acid encoding an autonomous heavy chain (AHC) variable domain; and (b) a plurality of pseudogenes that are operably linked to said functional immunoglobulin heavy chain gene and that donate, by gene conversion, nucleotide sequence to the nucleic acid encoding the AHC variable domain of (a), wherein the pseudogenes are upstream or downstream of the functional immunoglobulin heavy chain gene.

This disclosure provides, among other things, a transgenic chicken. In some embodiments, the transgenic chicken comprises B cells in which the endogenous immunoglobulin heavy chain locus comprises: (a) a functional immunoglobulin heavy chain gene comprising a nucleic acid encoding a heavy chain variable domain in which the CDR3 is in the range of 30-60 amino acids in length and comprises at least 2 cysteine residues; and (b) a plurality of pseudogenes that are operably linked to said functional immunoglobulin heavy chain gene and that donate, by gene conversion, nucleotide sequence to the nucleic acid encoding the heavy chain variable domain of (a), wherein the pseudogenes are upstream or downstream of the functional immunoglobulin heavy chain gene.

This invention belongs to the field of biotechnology and relates to a... ZmCASPL11 The application of genes in improving plant heat tolerance. ZmCASPL11 The gene encodes a protein belonging to the Casparian band structure family of membrane proteins (CASPL), whose expression level is significantly upregulated under high temperature stress. Mutants of this gene... ZmCASPL11 It exhibits a high-temperature sensitive phenotype. The present invention describes... ZmCASPL11 Genes were transformed into Arabidopsis thaliana and their function was validated. Compared with wild-type Arabidopsis thaliana, overexpression of [the gene] showed [significant improvement]. ZmCASPL11 The gene significantly enhances the tolerance of Arabidopsis thaliana plants to heat stress. This invention provides more theoretical basis for research on heat resistance in maize, and offers genetic resources and biotechnological means for the subsequent breeding of new heat-resistant maize lines and the innovation of germplasm resources.

The invention discloses a Trichoderma reesei engineering strain for producing trans-aconitic acid as well as a construction method and application of the Trichoderma reesei engineering strain, and relates to the technical field of biological engineering. The construction method comprises the following steps: transforming a coding gene of mitochondrial tricarboxylic acid transport protein and a coding gene of aconitic acid isomerase into a trichoderma reesei host bacterium, and carrying out gene recombination construction to obtain the trichoderma reesei engineering strain, the amino acid sequence of the mitochondrial tricarboxylic acid transporter is as shown in SEQ ID NO. 1; the amino acid sequence of the aconitic acid isomerase is as shown in SEQ ID NO. 2 or SEQ ID NO. 3. A new production strain is provided for microbial fermentation production of trans-aconitic acid, a large amount of trans-aconitic acid can be produced through direct fermentation by using common carbon sources such as glucose or cellulose, and the highest yield can reach 26.5 g / L.

This disclosure provides, among other things, strategies for minimizing antibody diversification in a transgenic animal that uses gene conversion for antibody diversification. In some embodiments, the animal may comprise a genome comprising an endogenous immunoglobulin light chain locus comprising: (a) a functional immunoglobulin light chain gene comprising a nucleic acid encoding a light chain variable region; and (b) a plurality of pseudogenes that are operably linked to the functional immunoglobulin light chain gene and that donate, by gene conversion, nucleotide sequence to the nucleic acid encoding a light chain variable region, wherein the pseudogenes are upstream or downstream of the functional immunoglobulin light chain gene and encode the same amino acid sequence as the light chain variable region of the functional immunoglobulin light chain gene of (a). In other embodiments, the locus may have a tandem array of coding sequences for the light chain.

The invention discloses a capsicum annuum cell cycle gene CaTAM and application thereof. The DNA sequence of the capsicum annuum cell cycle gene CaTAM is as shown in SEQ ID No.1. The gene is transformed into Columbia type arabidopsis thaliana through an agrobacterium flower immersion transformation method, a CaTAM heterologous expression arabidopsis thaliana strain is obtained, and it can be known that the heterologous expression of CaTAM can cause early bolting time of arabidopsis thaliana, increase of the number of leaves, decrease of the length and width of the leaves and increase of the root length. The expression of CaTAM of capsicum is silenced through a VIGS technology, a plant with reduced CaTAM expression is obtained, and it can be known that due to silencing of CaTAM, part of pollen cells exit meiosis in advance, secondary meiosis is not carried out, and large pollen grains are formed. It shows that CaTAM plays an important regulation and control role in meiosis, bolting time regulation and control, leaf growth, primary root development and the like, and the gene can be applied to breeding of solanaceae vegetables and other horticultural plants and has a good application prospect.

The application discloses a Phyllostachys edulis PeBBX19 gene, a coding protein and application thereof, and belongs to the technical field of plant genetic engineering.The nucleotide sequence of the Phyllostachys edulis PeBBX19 gene disclosed by the application is shown as SEQ ID NO.1, and the amino acid sequence of the coding protein is shown as SEQ ID NO.2.The application constructs an overexpression vector of the Phyllostachys edulis PeBBX19 gene, transforms the constructed overexpression vector of the Phyllostachys edulis PeBBX19 gene into Arabidopsis, and cultivates, screens and obtains a transgenic Arabidopsis strain with delayed flowering time.The application discloses the function of the PeBBX19 gene for the first time, the transgenic Arabidopsis plant has delayed flowering time after the PeBBX19 gene is transformed into the Arabidopsis plant, obviously shows a late flowering phenotype, and the number of rosette leaves is increased; and the expression amount of a downstream regulation gene FT is significantly reduced.

This disclosure provides, among other things, a transgenic chicken. In some embodiments, the transgenic chicken comprises B cells in which the endogenous immunoglobulin heavy chain locus comprises: (a) a functional immunoglobulin heavy chain gene comprising a nucleic acid encoding a heavy chain variable domain in which the CDR3 is in the range of 30-60 amino acids in length and comprises at least 2 cysteine residues; and (b) a plurality of pseudogenes that are operably linked to said functional immunoglobulin heavy chain gene and that donate, by gene conversion, nucleotide sequence to the nucleic acid encoding the heavy chain variable domain of (a), wherein the pseudogenes are upstream or downstream of the functional immunoglobulin heavy chain gene.

The invention discloses a ceramic cultural and creative product design and generation method. The method comprises the following steps: culture gene extraction: extracting dominant culture genes and recessive culture genes from target cultural heritage; user demand analysis: carrying out demand investigation on a target user based on a rooting theory, constructing a user demand emotion profile through an emotion calculation technology, and obtaining quantization intensity of user emotion dimensions; the material character elements are converted into external characterization of a product through a symbol conversion method, space narrative genes are converted into interactive logic or experience processes of the product, quantitative mental concepts and user demand emotion profiles are subjected to matching analysis, and emotion resonance design is conducted through an emotion migration method; and product generation and resource management: generating a ceramic cultural and creative product design scheme based on the translated design elements, and monitoring and evaluating the resource pushing condition in the design process.

The invention discloses a promoter PSCBV-FN39 and application of the promoter PSCBV-FN39. The nucleotide sequence of the promoter PSCBV-FN39 is as shown in SEQ ID NO: 1. The promoter disclosed by the invention is a rhizome tissue high-expression promoter, can be applied to gene transformation of various crops, preparation of transgenic plants and breeding of the transgenic plants, and has an important value for promoting acquisition of plant strains with high quality, high yield, high disease resistance, high stress resistance and other special characters; the development of a modern genetic breeding technology can be effectively promoted.

The application provides a Chinese cabbage root knot disease related gene BrSWEET14a, a construction method and application, belongs to the technical field of plant genetic engineering, and particularly relates to the following technical scheme. The CDS sequence of the Chinese cabbage BrSWEET14a is shown as SEQ ID No. 1. The gene is transformed into Col-type Arabidopsis thaliana through an Agrobacterium flower dipping transformation method, and a BrSWEET14a heterologous expression Arabidopsis thaliana strain is obtained. It is found that the T-DNA insertion mutant of the BrSWEET14a homologous gene Atsweet14 is more resistant to root knot disease than the Col-type, and the heterologous expression of the Chinese cabbage BrSWEET14a leads to the decrease of the disease resistance of Arabidopsis thaliana to the root knot fungus. It can be seen that the Chinese cabbage BrSWEET14a is closely related to the root knot disease, and the gene and the homologous genes of the gene in other cruciferous plants can be applied to the breeding of cruciferous plants and other horticultural plants, and have a good application prospect.

The invention provides application of a TaPAL8 gene in positive regulation of phenolic acid content of wheat, and belongs to the technical field of gene engineering. Wide targeted metabolome detection is carried out on wheat whole growth period materials by utilizing an HPLC-MS / MS technology, specific accumulation of various phenolic acids in stalks is highly related to expression of multiple phenylpropane pathway genes, one gene is annotated as phenylalanine ammonialyase, the gene number is TraesCS6D01G212200, and the gene is named as TaPAL8. According to the embodiment of the invention, the gene TaPAL8 is transformed into a wheat variety Fielder to obtain a transformed plant with overexpression TaPAL8, and in the overexpression mutant, the content of various acyl modified quinic acid substances in stalks and grouted grains is remarkably increased.

The invention relates to an application of a corn ZmXTH27 gene in improving the cold resistance of plants. The ZmXTH27 gene encodes a protein of a xyloglucan endotransglycosidase / hydrolase (XTH) family, the gene expression level of the ZmXTH27 gene is remarkably increased under low-temperature stress, and a mutant zmxth27 of the gene shows a low-temperature sensitive phenotype. The ZmXTH27 gene is transformed into arabidopsis thaliana and is subjected to functional verification, and compared with wild type arabidopsis thaliana, the tolerance of an arabidopsis thaliana plant over-expressed with the ZmXTH27 gene to cold stress is remarkably enhanced. According to the invention, more theoretical bases are provided for corn cold resistance research, and gene resources and biotechnological means are provided for subsequent cultivation and germplasm resource innovation of new cold-resistant corn strains.

The invention provides a tongue squamous cell carcinoma assessment method based on targeted sequencing and clinical characteristic parameters, and relates to the technical field of tongue squamous cell carcinoma assessment computer processing programs, the method comprises the following steps: S01, obtaining a tumor tissue sample of a tongue squamous cell carcinoma patient, and detecting gene mutation data through a targeted sequencing technology; s02, screening mutant genes significantly related to clinical pathological characteristics of the patient to obtain first N genes with the highest mutation frequency; s03, constructing a prognosis risk scoring model, converting the first N genes with the highest mutation frequency into a binary mutation state, then taking the binary mutation state as a variable, analyzing the training model through multivariable Cox proportional risk regression, and calculating the risk score of the patient; and S04, judging a principle according to a risk median. According to the invention, an individualized risk assessment tool is provided for clinic, and a doctor is assisted to formulate a more reasonable treatment and follow-up visit scheme, so that the prognosis and survival outcome of a patient are effectively improved.

The invention belongs to the technical field of plant breeding, and particularly relates to halophyte sea barley HmSTG2 protein or a coding gene thereof and application of the halophyte sea barley HmSTG2 protein in regulation and control of plant salt tolerance. The encoding protein of the HmSTG2 gene is positioned in vacuole, and plays an important role in maintaining low Na < + > and high K < + > concentrations of the overground part of a plant so as to resist external high salt stress, and the gene can be transformed into the plant, so that the salt tolerance of the transgenic plant can be effectively improved.

The present invention provides a fluorescent protein screening marker staygold‑DUF350 r The invention relates to a gene and its derivative products and applications, which belong to the field of genetic engineering technology. staygold‑DUF350 r fluorescent protein gene Staygold and genes DUF350 The codon for L-valine in the nucleotide sequence of is replaced with the second rare codon GTC, and the resulting fluorescent protein gene after codon replacement is Staygold The gene DUF350 after codon replacement is connected with a flexible protein peptide. staygold‑DUF350 r When the gene is transformed into Escherichia coli, the fluorescence intensity of the expression is positively correlated with the L-valine concentration, which can significantly improve the screening efficiency of L-valine high-yielding strains. staygold‑DUF350 r Genes can effectively save screening time and costs, and also improve screening efficiency.

The invention discloses a corn HPPD mutant and application of the corn HPPD mutant in improvement of plant herbicide tolerance. The invention specifically discloses a mutant protein, and the amino acid sequence of the mutant protein comprises mutation at at least one of the following positions relative to the amino acid sequence as shown in SEQ ID NO: 1: 18th, 20th and 26th sites. The invention also discloses an application of the mutant protein in improving the tolerance of plants to HPPD inhibitor herbicides (such as mesotrione). Experiments show that when the coding gene of the mutant protein is used for transforming rice, the mesotrione tolerance of a transgenic plant can be remarkably improved, and the mesotrione tolerance is improved by four times compared with that of a wild plant. Good gene resources are provided for herbicide-resistant crop breeding, HPPD inhibitor herbicide resistance genes are enriched, a foundation is laid for cultivating mesotrione-resistant new germplasm, and potential market value and wide application prospects are achieved in the agricultural field.

The invention provides a gene, a biological material and a method for regulating and controlling histidine content in crops, and belongs to the technical field of gene engineering. The invention provides a TaATL15 gene for regulating and controlling the histidine content in crops, and the amino acid sequence of protein coded by the TaATL15 gene is shown as SEQ ID No.3. The TaATL15 gene is transformed into wheat through a genetic transformation method by utilizing the TaATL15 gene, so that a TaATL15 overexpression transgenic plant is obtained, and the histidine content in the crops is regulated and controlled. And the contents of histidine, tyrosine and levodopa in the TaATL15 overexpression transgenic plant are obviously increased.

The invention discloses an application of a cotton GbCG1 gene in improvement of plant genetic transformation efficiency. Relates to the technical field of transgenosis and provides specific application. According to the invention, a somatic embryo regeneration candidate gene GbCG1 is screened out. The gene is only expressed in sea island cotton, overexpression of GbCG1 in upland cotton can significantly improve the callus differentiation efficiency and shorten the somatic embryo generation period, only 4-5 months are needed from transformation to seedling emergence, the time is 3-5 months faster than that of a control group, and the transformation efficiency is 3.5 times that of the control group. The invention discloses application of a GbCG1 gene in promoting somatic embryogenesis of cotton and improving the gene transformation efficiency, and the callus differentiation efficiency can be remarkably improved by overexpressing the GbCG1 gene. And valuable gene resources are provided for genetic breeding. The method can effectively solve the problem of cotton transformation genotype limitation, and broadens the variety types of cotton genetic transformation receptors.

The application relates to the field of plant genetic engineering, and specifically discloses a high-efficiency gene transformation system based on the stem tip meristem of a yellowing seedling of Zhongmian 113. The core components of the system include L1 medium suitable for Zhongmian 113, Agrobacterium GV3101 carrying a target gene, a special resuspension solution containing acetosyringone and Silwet-L77, and special primers for overexpression and gene editing; and the preparation method is as follows: seed disinfection and germination are performed to obtain a yellowing seedling, the stem tip is cut in an X shape to expose the meristem, Agrobacterium infection is performed, and then dark culture and light culture are performed, and after transplanting and cultivation to the true leaf stage, positive plants are screened and identified through PCR; the system can be used for gene transformation of Zhongmian 113 and improvement of high yield, high quality, disease resistance and the like, and has the advantages of strong adaptability, high transformation efficiency and short cycle; in addition, the preparation method of the application does not need callus regeneration, is simple and convenient to operate, has a standardized process, is good in repeatability and stability, and can quickly realize transgenic seed acquisition.

The invention discloses an agrobacterium tumefaciens-mediated fig fruit transient gene transformation method, which is characterized in that a carpopodium of a fig fruit is immersed in an agrobacterium tumefaciens infection solution carrying a target gene for dip dyeing, so that the infection efficiency and the positive rate of a transgenic fruit are remarkably improved. According to the method, a carpopodium infiltration type infection system is adopted, gene delivery carried by agrobacterium is achieved through the active absorption effect of a plant vascular system, and the infection efficiency is guaranteed. Compared with a traditional fig fruit injection method, the system avoids tissue browning caused by mechanical damage, the experiment is carried out in a tissue culture room, the operation is simple, the test period is short, and the method is suitable for efficiently and rapidly verifying gene functions in fig fruits.

This disclosure provides, among other things, strategies for minimizing antibody diversification in a transgenic animal that uses gene conversion for antibody diversification. In some embodiments, the animal may comprise a genome comprising an endogenous immunoglobulin light chain locus comprising: (a) a functional immunoglobulin light chain gene comprising a nucleic acid encoding a light chain variable region; and (b) a plurality of pseudogenes that are operably linked to the functional immunoglobulin light chain gene and that donate, by gene conversion, nucleotide sequence to the nucleic acid encoding a light chain variable region, wherein the pseudogenes are upstream or downstream of the functional immunoglobulin light chain gene and encode the same amino acid sequence as the light chain variable region of the functional immunoglobulin light chain gene of (a). In other embodiments, the locus may have a tandem array of coding sequences for the light chain.

This invention discloses the application of the jujube ZjUVI4 gene in rice grain shape improvement, belonging to the field of plant genetic engineering technology. The invention cloned the coding region sequence of ZjUVI4 from the jujube variety 'Dongzao' using PCR. The full-length sequence is 774 bp, encoding 257 amino acids. The nucleotide sequence of the coding region of the ZjUVI4 gene is shown in SEQ ID NO:1. Then, an overexpression vector was constructed, and transgenic experiments were conducted using the rice variety 'Zhonghua 11' as the transformation recipient. The ZjUVI4 gene was heterologously overexpressed in rice, and its biological function in the positive regulation of rice grain shape was investigated. This invention reveals the biological function of the jujube ZjUVI4 gene in positively regulating seed size. By transforming the ZjUVI4 gene into rice, the transgenic rice lines showed a significant increase in grain length and thousand-grain weight, while grain width remained largely unchanged, providing a new gene resource for rice grain shape improvement and high-yield, high-quality breeding.