164 results about "Phytase" patented technology

The invention relates to animal feeds and feed premixes containing synergistically effective amounts of betaine hydrochloride and a phytase.

The invention relates to the technical field of ecological agriculture, and discloses a phytase fungicide fertilizer as well as a production method and application thereof. 1-10 parts of a phytase high expression strain; 1-8 parts of a phytase solution; 1.7 to 9 parts of an embedding material; 3-8 parts of pH buffer particles; 10-30 parts of a micro-carbon carrier; 0.02 to 0.10 part of an indoleacetic acid microcapsule; and 30-60 parts of humus powder. The invention further provides a production method of the phytase fungicide fertilizer. The method comprises the following steps: S1, performing liquid fermentation on a phytase high-expression strain; s2, mixing the thalli with an enzyme solution; s3, adding pH buffer particles into the embedded particles; s4, drying the granules in a spray drying manner; and S5, mixing the dried product with humus powder, and granulating. By constructing a microbial inoculum system containing a phytase high-expression strain, efficient activation and conversion of organic phosphorus resources are realized, and the release efficiency of the fertilizer to phosphorus resources is improved.

The invention belongs to the technical field of green prevention and control of agricultural pests, and particularly relates to application of a phytase gene set of orius guttatus in the aspect of RNAi-mediated pest prevention and control. The gene set comprises a gene RP382, a gene RP382L1, a gene RP382L2 and a gene RP382L3, amino acid sequences of proteins coded by the gene RP382, the gene RP382L1, the gene RP382L2 and the gene RP382L3 are sequentially shown as SEQ ID NO: 5-8, and nucleotide sequences of the gene RP382, the gene RP382L1, the gene RP382L2 and the gene RP382L3 are sequentially shown as SEQ ID NO: 1-4. According to the invention, through systematic analysis, four phytase gene family members are identified in the Eriomycorus lividus for the first time, and the phytic acid metabolism capability of pests is destroyed by interfering the expression of the genes, so that the death rate of the pest Eriomycorus lividus is obviously increased to 50%.

The invention relates to an enzyme and bacterium combined feed additive for pre-digestion of feed raw materials as well as preparation and application of the enzyme and bacterium combined feed additive. The feed additive comprises: (a) an enzyme preparation, which is composed of protease, cellulase, xylanase, mannase, phytase, pectinase and keratinase, and has a total enzyme activity of 42500-45000 U / g; the probiotics are composed of bacillus subtilis, lactobacillus plantarum and saccharomyces cerevisiae, and the viable count is larger than or equal to 107 CFU / g; and (c) the mass ratio of enzyme to bacteria is (1-2): 1. According to the additive, the enzyme preparation and the probiotics are jointly used, and the raw materials are effectively pretreated, so that the nutritional value and the digestibility of the feed are improved. The feed additive disclosed by the invention comprises a specific enzyme preparation and probiotic combination, and predigestion treatment on the feed raw materials is realized through an optimized preparation process and fermentation process, so that the nutritional value and the utilization value of the feed raw materials are improved. The additive is simple in preparation method, low in cost and environment-friendly, and has a wide application prospect.

The invention discloses a repairing agent for repairing a heavy metal polluted solution by strengthening enzymatic phosphate precipitation under an acid rain condition, the repairing agent comprises sodium alginate, phytase and sodium glycerophosphate, and the mass ratio of the sodium alginate to the phytase to the sodium glycerophosphate is (1-3): (2-5): 50. The invention further discloses a method for repairing a heavy metal polluted solution by adopting the repairing agent. The repairing agent disclosed by the invention has the characteristic of higher enzyme activity when being applied under an acid rain condition, phosphate ions required by phosphate precipitation can be formed, and the repairing agent is remarkably beneficial to strengthening the stability of the precipitation; the heavy metal ions are adsorbed through functional groups carried on the surface of sodium alginate, removal of the heavy metal ions in the solution is accelerated, the risk of migration and diffusion of the heavy metal ions can be greatly reduced, and favorable conditions are created for improving the curing efficiency.

The invention discloses a preparation method of a low-protein biological fermentation feed for crayfish, and belongs to the technical field of aquaculture feeds, and the preparation method comprises the following steps: high-temperature fermentation: uniformly mixing soybean meal, rapeseed meal, cottonseed meal and a mixed bacteria solution containing bacillus subtilis, lactobacillus plantarum, saccharomycetes and EM bacteria, and performing high-temperature fermentation to obtain a fermentation product; and performing low-temperature fermentation: adding shrimp shell meal, corn flour, rice bran, cellulase and phytase into the fermentation product, performing uniform stirring to obtain a mixture, and performing low-temperature fermentation on the mixture to obtain the product. According to the low-protein biological fermentation feed disclosed by the invention, by-products such as shrimp shell meal, rice bran and rapeseed meal are used as raw materials of the biological fermentation feed, so that the cost of the feed can be reduced; and the aims of enhancing the nutritional quality, improving the immunity and oxidation resistance of the organism and improving the environment of a culture water body can be fulfilled for culturing the crayfish with the specification of about 12 grams per crayfish.

The invention discloses a neutral phytase mutant as well as a gene and application thereof, and belongs to the technical field of enzyme engineering. The invention aims to solve the technical problems that the existing neutral phytase is poor in thermal stability, poor in acidic environment stability, narrow in pH application range, low in low-temperature catalytic efficiency and the like. According to the technical scheme, the phytase preparation PHY06 with high stability and the gene and application of the phytase preparation PHY06 are provided. The method comprises the following steps: carrying out T329G, D333V, T353F and A364F mutation on a PHY03 sequence of which the amino acid sequence is shown as SEQ ID NO.1, so as to obtain a mutant PHY06;

The invention relates to the technical field of microbial fermentation, and particularly discloses a microbial fermentation process for food processing. The composition used in the process comprises lactobacillus plantarum, aspergillus oryzae and a compound enzyme preparation containing neutral protease, cellulase and phytase. The preparation method comprises the following steps: firstly, pretreating the raw materials, then compounding lactobacillus plantarum and aspergillus oryzae into a fermentation starter according to a specific proportion, fermenting in a liquid environment, and implementing dynamic stepped oxygen control; adding a complex enzyme preparation in a hypha growth period; then transferring into solid matrix conversion to construct a porous structure; and finally, carrying out two-stage temperature-controlled vacuum drying to obtain the product. The composition disclosed by the invention can be used for fermenting plant-based raw materials such as beans and grains, and has the advantage that the availability of a substrate can be improved through the synergistic interaction of bacteria and enzymes; in addition, according to the process, through stepped oxygen control, the double-bacterium respiratory rhythm can be precisely adapted, and the problem of metabolism mutual exclusion in a constant oxygen mode is thoroughly solved.

The present invention relates to the technical field of bacterial enzyme fermentation, and provides a bacterial enzyme synergistic fermentation method for removing antinutritional factors in soybean meal, the method comprises the following steps: S1, soybean meal pretreatment: soybean meal, water and an auxiliary agent are mixed and then subjected to steam explosion treatment, and the auxiliary agent comprises citric acid, a hydrogen peroxide solution and polyphenol; s2, bacterial enzyme fermentation: mixing the pretreated soybean meal with molasses and water to obtain a raw material, adding a complex microbial inoculant and a complex enzyme, uniformly stirring, and fermenting; the complex microbial inoculants comprise saccharomycetes, bacillus subtilis, lactobacillus rhamnosus and pediococcus pentosaceus; the compound enzyme comprises acid protease, pectinase, phytase, glucanase, alpha-galactosidase and keratinase; and S3, after fermentation is finished, drying the materials to obtain the fermented soybean meal. According to the fermentation method, the glycinin degradation rate can reach 79% or above, the acid soluble protein content is increased to 26% or above, the anti-nutritional factor content is remarkably reduced, and the nutritional value is greatly increased.

The invention discloses a method for preparing live pig feed through mixed solid-state fermentation of feed rape and rapeseed meal. The method specifically comprises the following steps: harvesting feed rape from a full-bloom stage to a final bloom stage, adding rapeseed dregs, and uniformly mixing to obtain a fermentation substrate with the water content of 55-65%; spraying the compound enzyme bacterium preparation on the fermentation substrate, uniformly stirring, finally compacting by using a packer, sealing, packing and storing, and fermenting for 45 days; the compound enzyme bacterial preparation comprises a bacterial agent and an enzyme agent; the fungicide comprises lactobacillus plantarum, bacillus subtilis, saccharomyces cerevisiae and aspergillus niger; the enzyme agent is prepared from phytase, cellulase, hemicellulase, pectinase, tannase, xylanase and alpha-galactosidase. The feed rape and the rapeseed meal are subjected to mixed solid fermentation, so that the content of phytic acid, glucosinolate and crude fiber is greatly reduced, the content of crude protein is increased, and the nutritional quality of the feed is remarkably improved. The fermented feed is used for replacing part of corn, soybean meal and wheat bran, and the use amount of concentrated feed in live pig breeding is reduced.

The invention discloses a compound enzyme preparation and a preparation method thereof, and relates to the technical field of enzyme preparations. The compound enzyme preparation is at least prepared from the following components in parts by weight: 10 to 30 parts of xylanase, 15 to 35 parts of modified cellulase, 8 to 20 parts of glucanase, 5 to 15 parts of protease, 5 to 18 parts of amylase, 3 to 12 parts of pectinase, 5 to 25 parts of phytase and 15 to 40 parts of modified superoxide dismutase compound microspheres. The compound enzyme preparation provided by the invention is added into daily ration of ruminants, so that the apparent digestibility and growth performance of the ruminants on nutrients can be improved, meanwhile, safer and better meat quality can be obtained, the meat taste is improved, and higher economic value is obtained.

The invention provides a leavening agent and a preparation method thereof, and belongs to the technical field of leavening agents. According to the scheme, an efficient fermentation system is constructed through the synergistic effect of bacillus amyloliquefaciens, bacillus licheniformis, lactobacillus plantarum and saccharomyces cerevisiae, in the initial stage, the bacillus amyloliquefaciens secretes amylase and phytase degradation substrates, and conditions are created for the saccharomyces cerevisiae to metabolize monosaccharide to generate flavor substances and vitamins; in the middle stage, bacillus licheniformis decomposes protein to release amino acid, lactobacillus plantarum regulates pH through lactic acid metabolism to inhibit infectious microbes, and a multi-layer encapsulated compound enzyme preparation is introduced to synergistically degrade a complex substrate; in the later stage, fructooligosaccharide in the prebiotics-buffer stabilizer promotes proliferation of probiotics, mannitol and chitosan protect the activity of thalli and the function of metabolic enzymes, and the fermentation environment is stabilized; according to the whole system, the functionality, the environmental adaptability and the storage stability of the leavening agent are improved through flora synergistic metabolism, dynamic fermentation condition control and the action of the multifunctional additive, and the diversified requirements in industrial production are met.

The invention belongs to the technical field of food, and provides a special enzyme composition for infant soybean milk powder and soybean milk powder thereof. A three-stage directional enzymolysis technology is adopted, the three-stage directional enzymolysis technology comprises glutamine transaminase deamidation pretreatment, bacillus subtilis protease-trypsin-papain first-stage hydrolysis and aminopeptidase-phytase-beta-glucosidase second-stage hydrolysis, and conductivity control, carbon dioxide ventilation in-situ complexation and spray drying processes are combined. The free amino nitrogen of the product is increased by more than or equal to 20%, the proportion of 200-1500Da peptide fragments is more than or equal to 70%, the median particle size is 50-180 microns, the water activity is less than or equal to 0.30, the instant dissolving time is less than or equal to 30 seconds at 25 DEG C under the water hardness of 15-200ppm, the settling rate is less than or equal to 3% after standing for 30 minutes, and the zeta potential is-10 to-25mV. The palatability contradiction between deep hydrolysis and bitterness inhibition of the bean-based infant formula powder, the brewing contradiction between high-calcium reinforcement and instant stability and the process controllability contradiction between three-stage enzymolysis and ionic strength accumulation are effectively solved, and the bean-based infant formula powder has wide application value in the fields of bean-based infant formula food and vegetable protein nutritional products.

Certain one or more embodiments of the present disclosure are directed, inter alia, to recombinant (genetically modified) filamentous fungal cells (strains) that produce proteins of interest, methods and compositions for designing and constructing modified filamentous fungal cells that produce proteins of interest, for endogenous and / or heterologous proteins of interest (in modified filamentous fungal cells). The present invention relates to methods and compositions for expression / production / secretion / recovery and the like of recombinant filamentous fungi (e.g., phytase, lipase, glucoamylase, phospholipase, esterase, cellulase, hemicellulase, xylanase and the like), methods and compositions for the production of proteins of interest in recombinant filamentous fungi, and methods and compositions for the production of proteins of interest in recombinant filamentous fungi. Wherein the proteins produced and secreted into and / or recovered from the bouillon have a homogeneous and consistent N-linked glycosylation pattern, and / or a (unwanted) reduction in saccharification of one or more proteins of interest, and the like.

The present compositions and methods relate to thermostabilization of phytase with phosphate and increasing the recovery of phytase during the production of heat-treated food or animal feed pellets that contain phytase.

Engineered robust high Tm-phytase clade polypeptides and fragments thereof are described herein. Also described are methods of making such engineered robust high Tm-phytase clade and fragments thereof and use thereof in enhancing animal performance.

The invention discloses a phytase mutant AppA as well as a coding gene, a preparation method and application thereof. The phytase mutant AppA is obtained by mutating amino acids at the 137th site, the 185th site and the 255th site of wild type phytase AppA from asparagine, aspartic acid and tyrosine into valine, asparagine and aspartic acid respectively; the amino acid sequence and the nucleotide sequence of the gene are SEQ ID NO.1 and SEQ ID NO.2 respectively. The catalytic efficiency of the mutant enzyme AppA provided by the invention on sodium phytate is improved by 2.5 times compared with that of a wild enzyme AppA; under the condition of 70 DEG C, the half-life period of the mutant enzyme AppA is 3.4 times that of the wild enzyme AppA. The mutant enzyme AppA has the characteristic of high enzyme activity under a high-temperature condition, which indicates that the mutant enzyme AppA has an important application prospect in industrial production of a feed in which phytic acid needs to be degraded under the high-temperature condition.

The present application belongs to the technical field of fermented feed, and particularly relates to a fermented feed taking rice bran as main material and a preparation method thereof. High-quality protein and other nutritional components in rice bran are usually combined with substances such as phytic acid in rice bran, and are not easy to be digested and absorbed by the gastrointestinal tract of animals. In view of the above problems, the present application provides a fermented feed taking rice bran as main material, which comprises fermentation raw materials, fermentation seed liquid, phytase and acid protease. Research shows that, for the degradation of phytate in rice bran, in addition to the phytase in the fermented feed playing a role, the type and combination of strains in the fermented feed also promote the degradation of phytic acid (salt) in rice bran. The bacillus licheniformis, lactobacillus plantarum and saccharomyces cerevisiae preferred by the present application can further improve the degradation rate of phytic acid (salt) and protein in rice bran, improve the release amount of inorganic phosphorus and polypeptide in the fermented feed, and then improve the utilization rate of nutritional components in rice bran.

The invention belongs to the technical field of agricultural animal husbandry application, and particularly relates to a feed additive for reducing backfat thickness of fattening Min pigs, a preparation method and application. The feed additive for reducing the backfat thickness of the fattening Min pigs comprises the following components in percentage by weight: 0.25 to 1.0 percent of hyodeoxycholic acid, 21.0 to 31.7 percent of citric acid, 0.7 to 3.2 percent of neutral phytase, 0.1 to 1.4 percent of astragalus membranaceus, 0.3 to 1.4 percent of saccharin sodium salt, 1.0 to 8.2 percent of rice hull powder and 54.0 to 64.7 percent of zeolite powder. The preparation method of the feed additive for reducing the backfat thickness of the fattening Min pigs comprises the following steps: crushing hyodeoxycholic acid, citric acid, neutral phytase, astragalus membranaceus, saccharin sodium salt, rice hull powder and zeolite powder into 80-mesh particles; the preparation method comprises the following steps: weighing the raw material components according to the ratio, stirring in a stainless steel mixer with the mixing uniformity of less than or equal to 5% for 5-10 minutes, and quantitatively packaging to obtain the finished product. The additive in the feed from the late stage of Min pig fattening to slaughtering can effectively reduce the backfat thickness of sows during slaughtering, improve the expression level of related genes of muscle fibers, and further improve the meat quality of Min pigs.

The invention discloses a pig breeding feed composition based on bacterium-enzyme synergy. The pig breeding feed composition comprises a piglet feed, a growing pig feed and a fattening pig feed, the piglet feed, the growing pig feed and the fattening pig feed at least comprise bacillus coagulans, clostridium butyricum, phytase, amylase, trypsin and NSP enzyme, and the bacillus coagulans can produce lactic acid and bacteriostats to enhance the intestinal barrier function of bred pigs; the clostridium butyricum can produce butyric acid and can regulate the balance of intestinal flora; phytase can decompose phytic acid and release mineral substances such as phosphorus, calcium and zinc; amylase can hydrolyze starch into soluble sugar, so that the energy utilization rate is increased; trypsin is used for degrading protein into small peptide and amino acid, is adaptive to the high-protein requirement of bred pigs, and can degrade cell wall polysaccharide of plant feed, reduce chyme viscosity and release embedded nutrients together with NSP enzyme; the invention also discloses an application of the breeding feed composition for pigs in breeding pig products.

This document describes poultry diets containing phytase polypeptides or fragments thereof, in which the diet contains no or practically no inorganic phosphate.

The invention provides a sugarcane fermentation product, a food quality guarantee product, a preparation method and application, and particularly belongs to the technical field of food preservation. The preparation method of the sugarcane leavening comprises the following steps: crushing sugarcane, and performing first sterilization to obtain sterile sugarcane pulp; inoculating the compound bacteria into the sterile sugarcane pulp for fermentation; the compound bacteria comprise lactic acid bacteria, bifidobacterium bifidum and propionibacterium; a degerming compound enzyme is supplemented in the fermentation process; the compound enzyme comprises xylanase, sucrase, phytase and aminopeptidase; and after fermentation, carrying out secondary sterilization, filtering, and discarding filter residues to obtain the sugarcane fermentation product. The sugarcane leavening disclosed by the invention is prepared from natural raw materials, has good anti-corrosion and anti-oxidation effects, and can prolong the shelf life of food.

The invention relates to the technical field of enzyme engineering, and particularly discloses a phytase mutant and application thereof. The mutant T273K with improved heat resistance is obtained through rational design and site-directed mutagenesis screening on the basis of aspergillus tubingensis phytase. The thermal stability and catalytic ability of the mutant are obviously improved compared with those before mutation; after heat preservation is carried out for 90 min at the temperature of 60 DEG C, the relative enzyme activity is 69.4%, and compared with WT, the relative enzyme activity is improved by 20.4%; the specific activity is improved by about 77.78% compared with that of WT, and the catalytic efficiency kcat / Km is improved by 50.56% compared with that of WT; and the enzyme activity retention rate reaches 33.8% under the condition of 10% salinity. The phytase mutant T273K has the comprehensive characteristics of salt resistance, heat resistance and the like, the phytase mutant T273K can better exert the phytic acid enzymolysis effect in the food processing process, turbidity generated by phytic acid in liquid food such as soy sauce, vinegar, cooking wine and the like can be remarkably reduced, so that the product quality is improved, and the phytase mutant T273K has a wide application prospect.

The invention relates to the technical field of soybean meal processing, and discloses a soybean meal raw material treatment method, soybean meal and animal feed, the method comprises the following steps: (1) mixing a soybean meal raw material with a pretreatment reagent, and respectively carrying out first extraction, second extraction and solid-liquid separation to obtain a first solid phase; the pretreatment reagent comprises organic alcohol, citric acid, fructo-oligosaccharide and water; the temperature of the second extraction is 15-45 DEG C higher than that of the first extraction; (2) mixing the first solid phase with an enzyme preparation, performing enzymolysis, and performing solid-liquid separation on a product after enzymolysis to obtain a second solid phase; the enzyme preparation comprises phytase, cellulase and non-starch polysaccharide enzyme. By adopting the method disclosed by the invention, the content of antinutritional factors in the soybean meal raw material can be fully reduced, the protein content of the product is increased, the efficient utilization and low immunity characteristics of soybean protein are considered, and the enzymolysis soybean meal product with high protein, low antigen, low small peptide, low bad oligosaccharide, strong stability and good palatability can be obtained without excessive enzymolysis.

The invention provides a shredded coconut stuffing fermented pig feed and a preparation method thereof. The feed comprises 40-80 parts of shredded coconut stuffing, 13-20 parts of corn flour, 10-20 parts of soybean meal, 5-10 parts of fish meal, 1-3 parts of a fermentation accelerant and 0.5-2 parts of a compound functional microbial agent. The fermentation accelerant is composed of corn cob residues and glutamic acid residues according to the mass ratio of (2-4): 1, and the compound functional fungicide contains lactobacillus plantarum, aspergillus niger, saccharomyces cerevisiae, bacillus subtilis and clostridium butyricum. The preparation method comprises the following steps: drying and sieving the shredded coconut stuffing at 60-70 DEG C, mixing the shredded coconut stuffing with beta-glucanase, phytase and cellulase in a ratio of 1: (0.5-1), performing enzymolysis for 2-4 hours at 45-55 DEG C, performing sterilization and cooling, adding the fermentation accelerant, inoculating the compound functional microbial inoculum, performing aerobic propagation for 8-10 hours at 28-32 DEG C, performing anaerobic fermentation for 48-72 hours at 35-40 DEG C and humidity of 75-85%, and finally mixing the shredded coconut stuffing with the corn flour, the soybean meal and the fish meal to obtain the feed. The feed can be used for pig breeding.

Provided is a parent phytase variant, which relates to the technical field of protein engineering. The variant, relative to the parent phytase thereof, has one or more amino acid substitutions at positions corresponding to positions 295, 349, and 374 of SEQ ID NO: 1. Compared to the parent phytase, the variant has increased thermal stability.

The invention provides a preparation method and application of biochar immobilized phytase, and relates to the technical field of biological enzyme immobilization. The method comprises the following steps: firing collected pineapple leaves into biochar for later use; adding pectin into water to obtain a pectin aqueous solution; adding the charcoal component into the pectin aqueous solution, uniformly mixing, filtering, washing and drying to obtain pectin charcoal; adding a cross-linking agent into the pectin biochar for cross-linking, then stirring and mixing with a phytase solution, filtering, and freeze-drying to obtain the biochar immobilized phytase. The biochar immobilized phytase can effectively overcome the defects that exogenous phytase is prone to inactivation and loss and difficult to store in soil, the phosphorus dissolving effect of phytase is enhanced, meanwhile, the biological activity and richness of indigenous phosphate-solubilizing bacteria are increased, soil phosphorus circulation is accelerated, soil phosphorus pool mineralization is promoted in the biochemical aspect, and the phosphorus-solubilizing effect is improved. The soil conditioner is a more environment-friendly and efficient soil conditioner and phosphate fertilizer synergist.

This invention discloses a fermentation method for improving the flavor and stability of old dough, belonging to the field of food technology. The method includes: mixing activated *Candida cruzi*, *Lactobacillus brevis*, *Hansenula polymorpha*, *Yarrowia lipolytica*, and *Saccharomyces cerevisiae* in a specific ratio to obtain a compound bacterial solution; and fermenting coarse grains with this compound bacterial solution to obtain coarse grain steamed buns. This invention employs the synergistic fermentation of five specific bacterial strains. *Lactobacillus brevis* and *Candida cruzi* synergistically optimize the rheological properties of the dough, *Hansenula polymorpha* and *Saccharomyces cerevisiae* synergistically produce rich aroma compounds, and *Yarrowia lipolytica* secretes phytase and cellolytic enzymes to lower the glycemic index. The coarse grain steamed buns prepared by this invention contain 55-65 flavor compounds, and the glycemic index is estimated to be reduced to 48-56, achieving a stable and controllable fermentation process, a low-GI product, and a rich flavor.

The invention discloses a bio-enzyme water-soluble fertilizer and a preparation method thereof, and belongs to the technical field of fertilizers, the bio-enzyme water-soluble fertilizer comprises the following raw materials by weight: 30-50 parts of urea, 15-20 parts of potassium dihydrogen phosphate, 2-5 parts of ferrous sulfate heptahydrate, 0.5-2 parts of immobilized phytase, 0.1-0.3 part of water-soluble vitamin, 3-10 parts of amino-oligosaccharin, and 8-15 parts of humic acid. The bio-enzyme water-soluble fertilizer provided by the invention has the advantages of good solubility, comprehensive fertility, small dosage and high fertilizer utilization rate, water and fertilizer are used together, a four-effect-in-one system of nutrition, activation, stimulation and improvement can be formed, and the bio-enzyme water-soluble fertilizer shows remarkable effects in the aspects of increasing the fertilizer utilization rate, enhancing crop resistance and improving soil health.

The invention discloses a stylosanthes guianensis purple acid phytase gene SgPAPhya and a stylosanthes guianensis purple acid phytase gene SgPAPhyb capable of enhancing the aluminum toxicity stress resistance of a plant and application of the stylosanthes guianensis purple acid phytase gene SgPAPhyb, and the aluminum toxicity stress resistance of the plant can be remarkably improved through a transgenic plant in which the SgPAPhya gene and the SgPAPhyb gene are excessively expressed; the SgPAPhya gene and the SgPAPhyb gene play an important role in improving the aluminum toxicity stress resistance of the plants, can be used for improving crops through a transgenic technology and improving the aluminum toxicity stress tolerance of the plants, and have important guiding significance for subsequent cultivation of aluminum toxicity stress resistant crop new germplasm suitable for being planted in acid soil.