128 results about "Phytase" patented technology

The invention discloses a preparation method of a low-protein biological fermentation feed for crayfish, and belongs to the technical field of aquaculture feeds, and the preparation method comprises the following steps: high-temperature fermentation: uniformly mixing soybean meal, rapeseed meal, cottonseed meal and a mixed bacteria solution containing bacillus subtilis, lactobacillus plantarum, saccharomycetes and EM bacteria, and performing high-temperature fermentation to obtain a fermentation product; and performing low-temperature fermentation: adding shrimp shell meal, corn flour, rice bran, cellulase and phytase into the fermentation product, performing uniform stirring to obtain a mixture, and performing low-temperature fermentation on the mixture to obtain the product. According to the low-protein biological fermentation feed disclosed by the invention, by-products such as shrimp shell meal, rice bran and rapeseed meal are used as raw materials of the biological fermentation feed, so that the cost of the feed can be reduced; and the aims of enhancing the nutritional quality, improving the immunity and oxidation resistance of the organism and improving the environment of a culture water body can be fulfilled for culturing the crayfish with the specification of about 12 grams per crayfish.

The invention discloses a neutral phytase mutant as well as a gene and application thereof, and belongs to the technical field of enzyme engineering. The invention aims to solve the technical problems that the existing neutral phytase is poor in thermal stability, poor in acidic environment stability, narrow in pH application range, low in low-temperature catalytic efficiency and the like. According to the technical scheme, the phytase preparation PHY06 with high stability and the gene and application of the phytase preparation PHY06 are provided. The method comprises the following steps: carrying out T329G, D333V, T353F and A364F mutation on a PHY03 sequence of which the amino acid sequence is shown as SEQ ID NO.1, so as to obtain a mutant PHY06;

The invention relates to the technical field of microbial fermentation, and particularly discloses a microbial fermentation process for food processing. The composition used in the process comprises lactobacillus plantarum, aspergillus oryzae and a compound enzyme preparation containing neutral protease, cellulase and phytase. The preparation method comprises the following steps: firstly, pretreating the raw materials, then compounding lactobacillus plantarum and aspergillus oryzae into a fermentation starter according to a specific proportion, fermenting in a liquid environment, and implementing dynamic stepped oxygen control; adding a complex enzyme preparation in a hypha growth period; then transferring into solid matrix conversion to construct a porous structure; and finally, carrying out two-stage temperature-controlled vacuum drying to obtain the product. The composition disclosed by the invention can be used for fermenting plant-based raw materials such as beans and grains, and has the advantage that the availability of a substrate can be improved through the synergistic interaction of bacteria and enzymes; in addition, according to the process, through stepped oxygen control, the double-bacterium respiratory rhythm can be precisely adapted, and the problem of metabolism mutual exclusion in a constant oxygen mode is thoroughly solved.

The invention discloses a method for preparing live pig feed through mixed solid-state fermentation of feed rape and rapeseed meal. The method specifically comprises the following steps: harvesting feed rape from a full-bloom stage to a final bloom stage, adding rapeseed dregs, and uniformly mixing to obtain a fermentation substrate with the water content of 55-65%; spraying the compound enzyme bacterium preparation on the fermentation substrate, uniformly stirring, finally compacting by using a packer, sealing, packing and storing, and fermenting for 45 days; the compound enzyme bacterial preparation comprises a bacterial agent and an enzyme agent; the fungicide comprises lactobacillus plantarum, bacillus subtilis, saccharomyces cerevisiae and aspergillus niger; the enzyme agent is prepared from phytase, cellulase, hemicellulase, pectinase, tannase, xylanase and alpha-galactosidase. The feed rape and the rapeseed meal are subjected to mixed solid fermentation, so that the content of phytic acid, glucosinolate and crude fiber is greatly reduced, the content of crude protein is increased, and the nutritional quality of the feed is remarkably improved. The fermented feed is used for replacing part of corn, soybean meal and wheat bran, and the use amount of concentrated feed in live pig breeding is reduced.

The invention provides a leavening agent and a preparation method thereof, and belongs to the technical field of leavening agents. According to the scheme, an efficient fermentation system is constructed through the synergistic effect of bacillus amyloliquefaciens, bacillus licheniformis, lactobacillus plantarum and saccharomyces cerevisiae, in the initial stage, the bacillus amyloliquefaciens secretes amylase and phytase degradation substrates, and conditions are created for the saccharomyces cerevisiae to metabolize monosaccharide to generate flavor substances and vitamins; in the middle stage, bacillus licheniformis decomposes protein to release amino acid, lactobacillus plantarum regulates pH through lactic acid metabolism to inhibit infectious microbes, and a multi-layer encapsulated compound enzyme preparation is introduced to synergistically degrade a complex substrate; in the later stage, fructooligosaccharide in the prebiotics-buffer stabilizer promotes proliferation of probiotics, mannitol and chitosan protect the activity of thalli and the function of metabolic enzymes, and the fermentation environment is stabilized; according to the whole system, the functionality, the environmental adaptability and the storage stability of the leavening agent are improved through flora synergistic metabolism, dynamic fermentation condition control and the action of the multifunctional additive, and the diversified requirements in industrial production are met.

The invention belongs to the technical field of food, and provides a special enzyme composition for infant soybean milk powder and soybean milk powder thereof. A three-stage directional enzymolysis technology is adopted, the three-stage directional enzymolysis technology comprises glutamine transaminase deamidation pretreatment, bacillus subtilis protease-trypsin-papain first-stage hydrolysis and aminopeptidase-phytase-beta-glucosidase second-stage hydrolysis, and conductivity control, carbon dioxide ventilation in-situ complexation and spray drying processes are combined. The free amino nitrogen of the product is increased by more than or equal to 20%, the proportion of 200-1500Da peptide fragments is more than or equal to 70%, the median particle size is 50-180 microns, the water activity is less than or equal to 0.30, the instant dissolving time is less than or equal to 30 seconds at 25 DEG C under the water hardness of 15-200ppm, the settling rate is less than or equal to 3% after standing for 30 minutes, and the zeta potential is-10 to-25mV. The palatability contradiction between deep hydrolysis and bitterness inhibition of the bean-based infant formula powder, the brewing contradiction between high-calcium reinforcement and instant stability and the process controllability contradiction between three-stage enzymolysis and ionic strength accumulation are effectively solved, and the bean-based infant formula powder has wide application value in the fields of bean-based infant formula food and vegetable protein nutritional products.

The present compositions and methods relate to thermostabilization of phytase with phosphate and increasing the recovery of phytase during the production of heat-treated food or animal feed pellets that contain phytase.

The invention discloses a phytase mutant AppA as well as a coding gene, a preparation method and application thereof. The phytase mutant AppA is obtained by mutating amino acids at the 137th site, the 185th site and the 255th site of wild type phytase AppA from asparagine, aspartic acid and tyrosine into valine, asparagine and aspartic acid respectively; the amino acid sequence and the nucleotide sequence of the gene are SEQ ID NO.1 and SEQ ID NO.2 respectively. The catalytic efficiency of the mutant enzyme AppA provided by the invention on sodium phytate is improved by 2.5 times compared with that of a wild enzyme AppA; under the condition of 70 DEG C, the half-life period of the mutant enzyme AppA is 3.4 times that of the wild enzyme AppA. The mutant enzyme AppA has the characteristic of high enzyme activity under a high-temperature condition, which indicates that the mutant enzyme AppA has an important application prospect in industrial production of a feed in which phytic acid needs to be degraded under the high-temperature condition.

The present application belongs to the technical field of fermented feed, and particularly relates to a fermented feed taking rice bran as main material and a preparation method thereof. High-quality protein and other nutritional components in rice bran are usually combined with substances such as phytic acid in rice bran, and are not easy to be digested and absorbed by the gastrointestinal tract of animals. In view of the above problems, the present application provides a fermented feed taking rice bran as main material, which comprises fermentation raw materials, fermentation seed liquid, phytase and acid protease. Research shows that, for the degradation of phytate in rice bran, in addition to the phytase in the fermented feed playing a role, the type and combination of strains in the fermented feed also promote the degradation of phytic acid (salt) in rice bran. The bacillus licheniformis, lactobacillus plantarum and saccharomyces cerevisiae preferred by the present application can further improve the degradation rate of phytic acid (salt) and protein in rice bran, improve the release amount of inorganic phosphorus and polypeptide in the fermented feed, and then improve the utilization rate of nutritional components in rice bran.

The invention belongs to the technical field of agricultural animal husbandry application, and particularly relates to a feed additive for reducing backfat thickness of fattening Min pigs, a preparation method and application. The feed additive for reducing the backfat thickness of the fattening Min pigs comprises the following components in percentage by weight: 0.25 to 1.0 percent of hyodeoxycholic acid, 21.0 to 31.7 percent of citric acid, 0.7 to 3.2 percent of neutral phytase, 0.1 to 1.4 percent of astragalus membranaceus, 0.3 to 1.4 percent of saccharin sodium salt, 1.0 to 8.2 percent of rice hull powder and 54.0 to 64.7 percent of zeolite powder. The preparation method of the feed additive for reducing the backfat thickness of the fattening Min pigs comprises the following steps: crushing hyodeoxycholic acid, citric acid, neutral phytase, astragalus membranaceus, saccharin sodium salt, rice hull powder and zeolite powder into 80-mesh particles; the preparation method comprises the following steps: weighing the raw material components according to the ratio, stirring in a stainless steel mixer with the mixing uniformity of less than or equal to 5% for 5-10 minutes, and quantitatively packaging to obtain the finished product. The additive in the feed from the late stage of Min pig fattening to slaughtering can effectively reduce the backfat thickness of sows during slaughtering, improve the expression level of related genes of muscle fibers, and further improve the meat quality of Min pigs.

This document describes poultry diets containing phytase polypeptides or fragments thereof, in which the diet contains no or practically no inorganic phosphate.

The invention relates to the technical field of enzyme engineering, and particularly discloses a phytase mutant and application thereof. The mutant T273K with improved heat resistance is obtained through rational design and site-directed mutagenesis screening on the basis of aspergillus tubingensis phytase. The thermal stability and catalytic ability of the mutant are obviously improved compared with those before mutation; after heat preservation is carried out for 90 min at the temperature of 60 DEG C, the relative enzyme activity is 69.4%, and compared with WT, the relative enzyme activity is improved by 20.4%; the specific activity is improved by about 77.78% compared with that of WT, and the catalytic efficiency kcat / Km is improved by 50.56% compared with that of WT; and the enzyme activity retention rate reaches 33.8% under the condition of 10% salinity. The phytase mutant T273K has the comprehensive characteristics of salt resistance, heat resistance and the like, the phytase mutant T273K can better exert the phytic acid enzymolysis effect in the food processing process, turbidity generated by phytic acid in liquid food such as soy sauce, vinegar, cooking wine and the like can be remarkably reduced, so that the product quality is improved, and the phytase mutant T273K has a wide application prospect.

The invention relates to the technical field of soybean meal processing, and discloses a soybean meal raw material treatment method, soybean meal and animal feed, the method comprises the following steps: (1) mixing a soybean meal raw material with a pretreatment reagent, and respectively carrying out first extraction, second extraction and solid-liquid separation to obtain a first solid phase; the pretreatment reagent comprises organic alcohol, citric acid, fructo-oligosaccharide and water; the temperature of the second extraction is 15-45 DEG C higher than that of the first extraction; (2) mixing the first solid phase with an enzyme preparation, performing enzymolysis, and performing solid-liquid separation on a product after enzymolysis to obtain a second solid phase; the enzyme preparation comprises phytase, cellulase and non-starch polysaccharide enzyme. By adopting the method disclosed by the invention, the content of antinutritional factors in the soybean meal raw material can be fully reduced, the protein content of the product is increased, the efficient utilization and low immunity characteristics of soybean protein are considered, and the enzymolysis soybean meal product with high protein, low antigen, low small peptide, low bad oligosaccharide, strong stability and good palatability can be obtained without excessive enzymolysis.

The invention provides a shredded coconut stuffing fermented pig feed and a preparation method thereof. The feed comprises 40-80 parts of shredded coconut stuffing, 13-20 parts of corn flour, 10-20 parts of soybean meal, 5-10 parts of fish meal, 1-3 parts of a fermentation accelerant and 0.5-2 parts of a compound functional microbial agent. The fermentation accelerant is composed of corn cob residues and glutamic acid residues according to the mass ratio of (2-4): 1, and the compound functional fungicide contains lactobacillus plantarum, aspergillus niger, saccharomyces cerevisiae, bacillus subtilis and clostridium butyricum. The preparation method comprises the following steps: drying and sieving the shredded coconut stuffing at 60-70 DEG C, mixing the shredded coconut stuffing with beta-glucanase, phytase and cellulase in a ratio of 1: (0.5-1), performing enzymolysis for 2-4 hours at 45-55 DEG C, performing sterilization and cooling, adding the fermentation accelerant, inoculating the compound functional microbial inoculum, performing aerobic propagation for 8-10 hours at 28-32 DEG C, performing anaerobic fermentation for 48-72 hours at 35-40 DEG C and humidity of 75-85%, and finally mixing the shredded coconut stuffing with the corn flour, the soybean meal and the fish meal to obtain the feed. The feed can be used for pig breeding.

Provided is a parent phytase variant, which relates to the technical field of protein engineering. The variant, relative to the parent phytase thereof, has one or more amino acid substitutions at positions corresponding to positions 295, 349, and 374 of SEQ ID NO: 1. Compared to the parent phytase, the variant has increased thermal stability.

The invention provides a preparation method and application of biochar immobilized phytase, and relates to the technical field of biological enzyme immobilization. The method comprises the following steps: firing collected pineapple leaves into biochar for later use; adding pectin into water to obtain a pectin aqueous solution; adding the charcoal component into the pectin aqueous solution, uniformly mixing, filtering, washing and drying to obtain pectin charcoal; adding a cross-linking agent into the pectin biochar for cross-linking, then stirring and mixing with a phytase solution, filtering, and freeze-drying to obtain the biochar immobilized phytase. The biochar immobilized phytase can effectively overcome the defects that exogenous phytase is prone to inactivation and loss and difficult to store in soil, the phosphorus dissolving effect of phytase is enhanced, meanwhile, the biological activity and richness of indigenous phosphate-solubilizing bacteria are increased, soil phosphorus circulation is accelerated, soil phosphorus pool mineralization is promoted in the biochemical aspect, and the phosphorus-solubilizing effect is improved. The soil conditioner is a more environment-friendly and efficient soil conditioner and phosphate fertilizer synergist.

This invention discloses a fermentation method for improving the flavor and stability of old dough, belonging to the field of food technology. The method includes: mixing activated *Candida cruzi*, *Lactobacillus brevis*, *Hansenula polymorpha*, *Yarrowia lipolytica*, and *Saccharomyces cerevisiae* in a specific ratio to obtain a compound bacterial solution; and fermenting coarse grains with this compound bacterial solution to obtain coarse grain steamed buns. This invention employs the synergistic fermentation of five specific bacterial strains. *Lactobacillus brevis* and *Candida cruzi* synergistically optimize the rheological properties of the dough, *Hansenula polymorpha* and *Saccharomyces cerevisiae* synergistically produce rich aroma compounds, and *Yarrowia lipolytica* secretes phytase and cellolytic enzymes to lower the glycemic index. The coarse grain steamed buns prepared by this invention contain 55-65 flavor compounds, and the glycemic index is estimated to be reduced to 48-56, achieving a stable and controllable fermentation process, a low-GI product, and a rich flavor.

The invention discloses a bio-enzyme water-soluble fertilizer and a preparation method thereof, and belongs to the technical field of fertilizers, the bio-enzyme water-soluble fertilizer comprises the following raw materials by weight: 30-50 parts of urea, 15-20 parts of potassium dihydrogen phosphate, 2-5 parts of ferrous sulfate heptahydrate, 0.5-2 parts of immobilized phytase, 0.1-0.3 part of water-soluble vitamin, 3-10 parts of amino-oligosaccharin, and 8-15 parts of humic acid. The bio-enzyme water-soluble fertilizer provided by the invention has the advantages of good solubility, comprehensive fertility, small dosage and high fertilizer utilization rate, water and fertilizer are used together, a four-effect-in-one system of nutrition, activation, stimulation and improvement can be formed, and the bio-enzyme water-soluble fertilizer shows remarkable effects in the aspects of increasing the fertilizer utilization rate, enhancing crop resistance and improving soil health.

The invention discloses a stylosanthes guianensis purple acid phytase gene SgPAPhya and a stylosanthes guianensis purple acid phytase gene SgPAPhyb capable of enhancing the aluminum toxicity stress resistance of a plant and application of the stylosanthes guianensis purple acid phytase gene SgPAPhyb, and the aluminum toxicity stress resistance of the plant can be remarkably improved through a transgenic plant in which the SgPAPhya gene and the SgPAPhyb gene are excessively expressed; the SgPAPhya gene and the SgPAPhyb gene play an important role in improving the aluminum toxicity stress resistance of the plants, can be used for improving crops through a transgenic technology and improving the aluminum toxicity stress tolerance of the plants, and have important guiding significance for subsequent cultivation of aluminum toxicity stress resistant crop new germplasm suitable for being planted in acid soil.

This invention discloses a light-aroma baijiu made from mung beans and its fermentation control method, belonging to the field of baijiu brewing technology. The invention uses 80-85% red sorghum and 15-20% mung beans as raw materials. After dehulling and crushing, the mung beans are liquefied with low-temperature α-amylase (30-60 U / g) and mixed with sorghum. The mixture is then treated with phytase (10-20 U / g), moistened at high temperature, and steamed to gelatinize. After cooling, light-aroma low-temperature koji and ester-producing yeast are added to strengthen the koji. The mixture undergoes a two-stage variable-temperature fermentation in earthen vats: the first 7-10 days at 18-22℃, and the subsequent 18-21 days gradually increasing the temperature to 25-28℃, with a peak temperature ≤32℃ throughout the process, for a period of 28 days. The fermented mash is mixed with rice husks, loaded into stills, and distilled over a low flame. The distillate temperature is ≤25℃. 5-8% of the first and last sips are discarded, and the 65% vol middle section of the liquor is collected and aged in earthenware jars for more than 6 months. This invention constructs a three-stage fusel oil regulation system: "low-temperature liquefaction to control precursors, variable-temperature fermentation to inhibit formation, and slow-fire distillation to retain." The finished wine has a fusel oil content of ≤0.8g / L, a natural mung bean aroma, and a high level of comfort after drinking.

This invention relates to the field of poultry farming technology and discloses a method for reducing antibiotic use in chicken farming. This invention solves the problems of poor hygiene in the farming environment, high chicken disease rates, and the need for excessive antibiotic feeding. The method employs a small, frequent feeding approach, adding astragalus polysaccharide, phytase, fish meal, and meat and bone meal to the feed to enhance the chicken's immune function and reduce disease occurrence during the rearing process. Simultaneously, during feeding, the bottom plate is lowered, and an electric telescopic rod A and scraper are used to clean the manure on the baffle. Water is sprayed and air is blown onto the wire mesh frame during movement to remove impurities. When the feed reaches the bottom, a connecting pipe is used to change the water flow direction to clean the scraper. During ventilation, a reciprocating cleaning brush is used to remove down adhering to the mesh, preventing pathogens carried by the down from causing disease. Ultimately, this method ensures hygiene in the farming environment and reduces antibiotic use.

This invention belongs to the field of microbial technology, specifically relating to *Lactobacillus paracasei* YYS-EN2, which produces cellulase, pectinase, tanninase, and phytase, and its applications. This *Lactobacillus paracasei* YYS-EN2 is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 28531. The *Lactobacillus paracasei* YYS-EN2 provided by this invention possesses the ability to produce cellulase, pectinase, tanninase, and phytase, and can degrade plant cellulose and pectin, decompose tannins and phytic acid. When applied to fermented products, it can reduce bitterness and improve product clarity. It can be widely used in the production of fermented products, such as fermented plant-derived nutritional powders and fermented plant-derived polypeptide powders, and can also be used in probiotic products and functional foods. It colonizes the human intestinal tract, improving gut microbiota structure and promoting digestion and absorption.

The invention relates to a compound feed additive used in the laying period of breeding hens and a preparation method thereof, and belongs to the technical field of breeding hens breeding and hatching. The invention discloses a compound feed additive. Comprising the following components in parts by weight: 15 to 25 parts of clostridium butyricum, 25 to 35 parts of bacillus subtilis, 12 to 18 parts of bacillus licheniformis, 5 to 15 parts of vitamin B family, 2 to 4 parts of choline dihydrogen citrate, 2 to 5 parts of herba epimedii extract, 0.2 to 0.4 part of nano L-selenomethionine, 4 to 6 parts of bile acid, 5 to 8 parts of yeast cell wall polysaccharide, 2 to 5 parts of organic zinc, 5 to 15 parts of trace elements and 1 to 3 parts of phytase. Through scientific proportioning and synergistic effect of multiple functional components, the feed improves intestinal health of breeding hens, improves reproductive performance, enhances immunity, promotes digestion and absorption of the breeding hens to nutrient substances in the feed, improves feed conversion rate and reduces feed-egg ratio.

The invention belongs to the technical field of gene engineering and enzyme engineering, and particularly relates to a high-temperature-resistant phytase mutant as well as a preparation method and application thereof. The 19 , 104 and 305 genes of a phytase gene appA are modified to obtain a plurality of high-temperature-resistant phytase mutants, the heat resistance of the three mutants G19P / G104P / D305R is remarkably superior to that of wild type and traditional phytase, experimental results show that after the phytase mutants are respectively treated for 20 minutes at the high temperature of 80 DEG C, 90 DEG C and 100 DEG C, the residual rates of enzyme activity are respectively 94.6%, 91.8% and 66.5%, and the residual rates of enzyme activity are respectively 94.6%, 91.8% and 66.5%. The phytase can completely tolerate granulation at the high temperature of 80-95 DEG C in the feed industry, and the industrial pain point of insufficient enzyme activity after traditional phytase granulation is greatly solved.

This invention discloses a high-efficiency protein expression system, its construction method, and its applications. The system includes a heterozygous promoter activator and a heterozygous promoter. The heterozygous promoter activator consists of the DNA-binding protein TetR, a linker peptide, and the transcription activator Mit1-Δ60-96. The heterozygous promoter consists of the protein-binding sequence TetO, a spacer sequence, and a basal promoter. The linker peptide is (GGGGS)×2, the spacer sequence is TCGCGCGC, and the basal promoter is P. AOX1 The core promoter of this invention. In the absence of a heterozygous promoter activator, the target protein gene initiated by the heterozygous promoter is expressed very weakly; in the presence of a heterozygous promoter activator, the target protein gene is expressed efficiently. This invention's system has good applicability and scalability, and the expression intensity of various target proteins, including xylanase, phytase, and glucosidase, is significantly higher than that of commonly used P. AOX1 Promoter.

The application discloses a feed composition and a preparation method and application thereof, and relates to the technical field of feed additives.The feed composition comprises the following components in parts by weight: 4-6 parts of protease, 1-3 parts of phytase, 5-8 parts of bacillus subtilis, 5-8 parts of bacillus coagulans, 40-60 parts of oil, and 2-4 parts of emulsifier.The specific bacteria and enzymes are used for synergistic effect in the feed composition, so that the prepared feed can not only eliminate the muddy smell of farmed fish, but also reduce the abdominal fat rate of grass carp and improve the tightness of fish meat.

Engineered robust high Tm-phytase clade polypeptides and fragments thereof are described herein. Also described are methods of making such engineered robust high Tm-phytase clade and fragments thereof and use thereof in enhancing animal performance.

The invention discloses a special feed for breeding laying hens and a preparation method thereof, and belongs to the technical field of livestock and poultry feeds. The special feed for breeding the laying hens consists of a basal feed and a core functional compound. The core function compound innovatively comprises an organic trace element compound composed of ferric glycine, zinc methionine and selenium yeast, an antioxidant and absorption enhancer composed of astaxanthin and medium chain triglyceride (MCT), and an intestinal health and calcium and phosphorus metabolism regulator composed of sodium butyrate, beta-glucan and phytase. The key point of the preparation method is that MCT is adopted to pre-mix and coat the core functional compound components. Through synergism of all the components, the egg laying performance, egg quality, oxidation resistance and bone health of laying hens are effectively improved, and safe, efficient and environment-friendly laying hen breeding is achieved.

The invention discloses a Chinese style functional pastry and a preparation method thereof. Firstly, white kidney beans are induced to germinate, endogenous phytase is activated to reduce the content of resistance factors such as phytic acid and lectin, and the activity of an alpha-amylase inhibitor is protected by spraying a soybean trypsin inhibitor. And then mixing the germinated white kidney beans with the aqueous extracts of astragalus membranaceus and poria cocos, inoculating saccharomycetes and lactobacillus plantarum for fermentation to generate flavor substances and active components, and consuming small molecular sugar to maintain the low GI characteristic. Finally, puerarin powder and the like are added into the white kidney bean paste and the Chinese yam paste to prepare dough, the dough wraps the functional stuffing prepared from the chia seed powder, the Chinese wolfberry fruit powder and the red date and longan paste, and the finished product is obtained through forming, steaming and rose dew incense. The method effectively solves the contradiction between alpha-amylase inhibitor loss and anti-nutritional factor degradation in germination, improves the bioavailability, flavor level and intestinal health regulation function of the product, and realizes the synergistic interaction of nutrition enrichment, blood sugar management and multiple nourishing regulation.

The invention relates to the technical field of livestock and poultry breeding feed, and discloses a breeding mixed feed additive formula and a feeding method. The additive is prepared from core nutrient components (calcium sulfate, methionine and lysine), immunity enhancing components (astragalus polysaccharide, eucommia ulmoides extract and probiotic compound preparation), digestion promoting components (amylase, phytase and fructo-oligosaccharide) and flavor improving components (pericarp residue compound) according to a specific weight part ratio. The feeding method combines physiological characteristics of 1-4 week old chicks, 5-16 week old bred chickens and laying hens / broiler chickens in the fattening stage, and the mixing proportion, the feeding amount and the feeding frequency are adjusted in a targeted mode. The technical defects that an existing feed additive is single in nutrition, poor in immunity enhancement effect, insufficient in meat quality improvement and unscientific in feeding are overcome, balanced nutrition supply, immunity enhancement, chicken flavor optimization and feed utilization rate improvement of chickens are achieved, and the chicken raising industry is promoted to develop towards the efficient, high-quality and green direction.