2123 results about "Phytase" patented technology

The present invention belongs to the field of poultry feed technology, specifically to a meat large chicken feed and method for preparing the same. The meat large chicken feed is composed of the following components by weight parts: 30-80 parts of corn, 0-30 parts of wheat, 5-40 parts of bean pulp, 0-10 parts of peanut meal, 0-10 parts of cotton meal, 2-8 parts of corn protein powder, 0.5-5.0 parts of lipid, 1.0-6.0 parts of hypo-powder, 0.5-2.0 parts of mountain flour, 0.5-2.0 parts of calcium hydrogen orthophosphate, 0.2-0.4 parts of salt, 0.1-1.0 parts of complex vitamin; 0.1-1.0 parts of complex trace elements, 0.05-0.5 parts of micro-ecological formulation, 0.01-0.2 parts of Chinese herbal medicines additives, and 0.01-0.2 parts of phytase. The inventive meat large chicken feed has high digestibility, nutrient balance, low harmful components, and high security, so that the resistance of broiler chickens is increased and the pollution to the environment is significantly reduced.

The present disclosure relates to an aqueous process for the preparation of a protein isolate and a hydrolyzed protein concentrate from an oilseed meal, optionally comprising:

• • mixing an oilseed meal with an aqueous solvent to form a slurry;

optionally treating the slurry with phytase y;

separating the slurry with a solid/liquid separation to form:

• a liquid phase, comprising the aqueous solvent, soluble protein and oil; and
• a solid phase comprising insoluble protein;

separating the liquid phase to form:

• an oil phase; and
• an aqueous protein phase;

subjecting the aqueous protein phase to membrane filtration to obtain a protein solution; and drying the protein solution to obtain the protein isolate

subjecting the insoluble protein to enzymatic hydrolysis, and

subjecting the hydrolyzed protein to membrane filtration to obtain an amino acid and peptide solution; and drying the amino acid and peptide solution to obtain the hydrolyzed protein concentrate.

The invention relates to a layer foodstuff used for producing health care eggs, consisting of the following components in weight portion: 120 to 160 portions of calcium hydrogen phosphate, 250 to 350 portions of mountain flour, 50 to 60 portions of common salt, 20 to 40 portions of saleratus, 15 to 20 portions of choline, 35 to 40 portions of complex trace elements, 8 to 10 portions of complex vitamins, 10 to 15 portions of lysine, 25 to 30 portions of methionine, 220 to 400 portions of pine needle meal, 1 to 3 portions of bacillus subtilis, 8 to 10 portions of copper sulfate, 2 to 5 portions of phytase, 1 to 5 portions of yeast selenium, 3 to 6 portions of vitamin E, and 250 to 400 portions of Chinese traditional herbal medicine preparation. The invention adds nutritive additives etc. into the foodstuff; therefore the egg laid by the layer contains such beneficial elements as selenium, vitamin E, and is rich in Omega-3 polyunsaturated fatty acid and low in cholesterol.

The invention belongs to the field of feeds, and particularly relates to a six percent-premix for lactating sows and a wheat-type daily ration prepared from the six percent-premix. The premix comprises multivitamins, composite trace elements, DL-methionine, lysine, threonine, L-arginine, valine, composite electrolyte, compound enzyme, 5000 IU of phytase, functional additives, feed attractants, calcium hydrogen phosphate, stone powder, salt, choline, antioxidants and defatted rice bran. On the whole, the daily ration for the lactating sows is capable of protecting the development of mammary glands of the sows, ensuring plentiful milk with high quality in the lactation period of the sows and increasing the survival rate of suckling piglets, and enables the piglets to have higher weaning weight.

The present invention provides for the expression of phytase in transgenic plants or plant organs and methods for the production of such plants. DNA expression constructs are provided for the transformation of plants with a gene encoding phytase under the control of regulatory sequences which are capable of directing the expression of phytase. These regulatory sequences include sequences capable of directing transcription in plants, either constitutively, or stage and/or tissue specific, depending on the use of the plant or parts thereof. The transgenic plants and plant organs provided by the present invention may be applied to a variety of industrial processes either directly, e.g. in animal feeds or alternatively, the expressed phytase may be extracted and if desired, purified before application.

The invention belongs to the technology field of grain processing, specifically a method for producing active germinated brown rice and products thereof. The method comprises the following steps: screening brown rice raw material to remove impurity, washing, sterilizing, soaking in a nutrient fluid supplemented with calcium lactate, gibberellin, phytase, cellulase and vitamin C for 10-36 hours at 10-40 DEG C, draining, incubating for 10-36 hours at 10-40 DEG C under relative humidity of 50-90% to allow germination, drying with hot air of 40-55 DEG C for 0.5-3 hours until the sprout grows about 0.5-1.2 mm in height, drying with 2-10 w/g microwave for 2-10 minutes until the water content of germinated brown rice is below 15%. Compared with the prior art, the germinated brown rice is nutritious, retains almost all nutrients of brown rice and has high nutritional value.

The invention discloses probiotic type premix feed for laying hens, which is prepared from vitamin compound premix feed for the laying hens in laying period, a bacillus and lactobacilli compound preparation, xylan and mannan complex enzyme, phytase, methionine, lycine, choline chloride, common salt, calcium hydrophosphate, stone dust, fish meal, corn gluten meal, sodium bicarbonate, trace element compound premix feed for the laying hens in the laying period, ethoxyquinoline, bentonite, and rice chaff by mixing in proportion. Compared with the common compound feed for the laying hens, the compound feed prepared by the premix feed reduces discharge of harmful substances such as nitrogen and phosphorus in chicken manure by more than 20 percent, reduces the concentration of harmful gases such as ammonia in a hen house by more than 40 percent, improves the feed utilization rate by 5 to 10 percent and the laying rate by 3 to 5 percent, averagely prolongs the laying period by 25 days, and reduces feeding cost by 5 to 8 percent and the death-cull rate of the laying hens.

The invention discloses a Chinese medicinal herb additive for egg chickens and application. The Chinese medicinal herb additive comprises membranous milkvetch root, eucommia bark, honeysuckle, jiuyue, Weeping Forsythiae Capsule, szechwon tangshen root, sanguisorba, hawthorn, Indian Buead, officinal magnolia bark, Chinese pulsatilla root, giant knotweed rhizome, ricepaperplant pith, largehead atractylodes rhizome, tangerine peel, malt, indigowoad root, swordlike atractylodes rhizome, indigowoad leaf, three immortals, liquorice, cyrtomium fortune, nutgrass galingale rhizome, chatoyancy, calamus and pinellia tuber, and mixture of the compositions is added with calcium bicarbonate, phytase, vitamin E and methionine. The Chinese medicinal herb additive effectively promotes absorption of nutrient, can prevent and control diseases, strengthen quality of eggshells, reduce the broken egg rate and soft-shell eggs, abnormal eggs and sand shell eggs, can effectively prevent bird flu, prevent and control diseases such as laxness, feather pecking, hair shedding, anus pecking, salpingitis, respiratory disease and the like, can achieve good effect with a dosage of about 4 percent, cannot damage physiological functions of the egg chickens, and can prolong egg laying peak season, improve color of the eggshells and the laying rate by 8 to 20 percent, and increase weight of eggs.

The invention discloses freshwater fish feed and a preparation method thereof. The feed comprises the following materials in percentage by weight: 5%-17% of import fish powder, 8%-22% of flour, 3%-16% of potato starch, 3%-18% of beer yeast, 5%-15% of bean pulps, 5%-15% of peanut pulps, 5%-10% of rape seed cakes, 2%-8% of corn powder, 3%-9% of cooked egg protein, 5%-8% of dried meat floss powder, 1%-2% of liquid phytase, 1%-3% of non-starch polysaccharase, 0.5%-1.9% of glutamine, 1%-2% of microecological preparation and 1%-3% of immune polysaccharides. The preparation process comprises a crushing step, a mixing step, a pelletizing step and a spraying step. The freshwater fish feed has the beneficial effects of being capable of well satisfying the nutrition needs of freshwater fish, improving the immunity of the freshwater fish, prompting intestinal absorption and improving the production performance of the freshwater fish.

The invention discloses a laying hen composite premix, and belongs to the technical field of feed composition. A composite enzyme preparation containing beta-mannase, beta-glucanase, acidic proteinase, phytase, pectinase, and amylase is adopted to eliminate the anti-nutrient factors in soybean meal. Yeast cell wall type mycotoxin absorbent is used to effectively eliminate the hazardous factors that may affect the intestine health and are caused by using a large amount of corns. A stomach fortifying acid is used to effectively adjust the pH value of intestines. The used wood chips in the premix are soft and high in fiber content, do not hurt the intestines, do not pollute the environment, can effectively strengthen the digesting and absorbing abilities of laying hens, and have a stronger bearing and water-absorbing performance. The materials mentioned above are systematically and scientifically used to guarantee that the intestines of the laying hens be in the best state. The premix can improve the immunity and disease resistant performance of laying hens, and makes the laying hens exert the maximal laying ability. Moreover, the water content of manure is reduced, and the economic profit of farms that use the premix is prominently improved.

The present invention provides one kind of improved high specific activity phytase and its coding gene, and the improved phytase has even higher heat stability. The present invention also provides recombinant yeast cell containing the phytase gene of the present invention. The recombinant yeast of the present invention has phytase expressing amount up to 6.5E6 U in each ml of fermented liquid. The phytase of the present invention may be used widely in feed and food industry.

The invention relates to a compound enzyme preparation for fermentation of pu'er tea, which is formed by compounding 10% to 20% of cellulase, 10% to 20% of pectinase, 5% to 15% of polyphenol oxidase, 5% to 10% of glucose oxidase, 5% to 10% glucoamylase, 5% to 10% xylanase, 5% to 10% of beta-glucanase, 5% to 10% of beta-mannase, 5% to 10% of tannase, 5% to 10% of acidic protease, 5% to 10% of lipase, 1% to 5% of alpha-amylase and 1% to 5% of phytase. By the aid of coordination of the enzyme system, polysaccharide and other substances in tea including cellulose and hemicellulose can be effectively decomposed, so that various physiochemical substances in cells can be dissolved. Meanwhile, oxidation and condensation of tea polyphenol, decomposition of protein, amino acid and carbonhydrate, a series of reactions of various products including polymerization and condensation and the like are accelerated and promoted.

The invention relates to a marine-fish environmental protective compound feed, consisting of the following materials in weight ratio: 13 to 20 portions of domestic fish powder, 8 to 15 portions of biological fish meal ABP600, 8 to 12 portions of soybean meal, 10 to 18 portions of peanut bran, 3 to 10 portions of corn, 3 to 7 portions of cotton seed meal, 3 to 7 portions of rapeseeds meal, 3 to 5 portions of meat and bone meal, 3 to 5 portionsof squid bowels powder, 20 to 30 portions of high gluten flour, 1.0 to 3.0 portions of blood meal, 1.0 to 2.0 portions of soybean lecithin, 2.0 to 3.0 portions of marine-fish oil, 0.3 to 0.6 portions of choline chloride (50%), 0.05 to 0.1 portions of Vitamin C phosphate (35%), 0.15 to 1.5 portions of calcium biphosphate, 0.2 to 0.5 portions of compound multiplex vitamin, 0.5 to 1.5 portions of compound mineral salt, 0.1 to 0.3 portions of feed attractant, 0.1 to 0.3 portions of micro-bio-preparation, 0.03 to 0.08 portions of A3Alpha peptidoglycan, 0.01 to 0.04 portions of phytase, and 0.04 to 0.08 portions of non-starch polysaccharase. The compound foodstuff can meet the nutrition demands of such high density cultivated marine fishes such as weever, large yellow croaker, American red fish, sparidae fishes, and trachinotus ovatus and rudd etc, and the cultivated objects can rapidly grow and has high foodstuff conversion rate; the compound foodstuff can intensify the immunity and anti-stress ability of fishes and endow them with good vitality, vivid body color and high survival rate; compared with marine fished cultivated by other foodstuffs, the excretion rates of nitrogen and phosphorous of the marine fishes cultivated by the compound foodstuffs is lowered by 10 to 15 percent, and cause smaller contamination to the cultivation water body.

The invention discloses a nutritional compound enzyme used for crops, and the application thereof. The nutritional compound enzyme comprises proteases, phytase, amylases, cellulase enzymes and pectinase, and the like, is a compound formula of a plurality of enzyme preparations, can replace a plant growth promoter and improve soil texture, can provide nutritional substances, such as nitrogen sources and phosphorus and the like, for the counterbalanced growth of crops, lead the crops to become strong and absorb sufficient nutrient at the later growth stage, can improve the resistance against diseases of the crops, is beneficial to the growth and maturation of the crops and improves the output of the crops; the nutritional compound enzyme can convert cellulose into a part of oligosaccharides for improving the immunity of the crops and increasing the chlorophyll content of functional leaves of the crops, thereby improving the quality and output of the crops. The nutritional compound enzyme brings significant economic benefits for peasants.

A process for preparing the edible fibre powder from wheat bran by multi-enzyme step treating method includes such steps as treating wheat bran sequentially by endogenous phytase, amylase, proteinase and lipase to obtain high-purity edible fibres, decoloring by sodium hypochlorite and hydrogen peroxide, squeezing, laying aside and superfine pulverizing.

The invention belongs to the animal sitology technical field, in particular to big pig premix and an application method thereof. The premix mainly comprises the following components: iron element, copper element, zinc element, manganese element, iodine element, cobalt element, selenium element, composite vitamine premix, DL-methionine, lysine, threonine, cholinehydrochloride, calcium hydrophosphate, rock flour, salt, carrier and phytase. The big pig premix and conventional basic ration are used to prepare big pig compound feed to feed big pigs with conventional process, and the content of the big pig premix in the big pig compound feed is 4% according to the weight percentage. The big pig premix provided by the invention is simple and safe to use; after feeding big pigs, the fodder-meat rate can be reduced, the big pig growth speed and lean meat percentage are increased and the meat quality safety can be improved.

The invention discloses a feed special for a laying period of a laying hen of a local variety, belonging to the technical field of chicken feed preparation. The feed comprises the following raw materials: 60-65 percent of corns, 2-10 percent of wheat bran, 18-25 percent of bean pulp, 0-1.0 percent of vegetable oil, 4-9 percent of mountain flour, 0.5-1.5 percent of calcium hydrophosphate, 0.2-0.5 percent of table salt, 0.01-0.05 percent of 65 percent lysine, 0.05-0.20 percent of methionine, 0.1-0.15 percent of 50 percent choline chloride, 0.01-0.02 percent of phytase, and 0.5-1 percent of premix. The feed is mainly used specific to the laying period of the laying hen of the local variety; the feed is fed directly in a powder form, so that the feed conversion rate can be effectively improved, the laying peak time can be prolonged, the egg quality can be improved, and the egg broken ratio can be reduced by 3-5 percent.

The invention discloses a preparation method of a broiler feed. The broiler feed belongs to the field of feeds. The broiler feed provided by the invention is prepared from high-quality white corn, bean cake, rape seed cake, cottonseed cake, barley, broomcorn, alfalfa meal, fish meal, mountain flour, plant oil, edible salt, traditional Chinese medicine additive agents, sodium bicarbonate, trace elements, allicin, phytase and vitamin C through the technology of crushing, mixing, granulating, drying and the like. The feed is rich in nutrition, has good mouthfeel, can meet the nutrition need of broilers in a growing period after being taken by the broilers so as to enable broilers to guide qi downward, be good in digestion and be clam, also has the effects of accelerating broilers to grow, and improving the chicken flavor and daily gain, is high in disease resistant capability, and is high in safety.

The invention relates to a general-purpose high-efficiency bulking compound feed for a culter alburnus and a pelteobagrus fulvidraco. The compound feed is prepared from the following raw materials in portions by weight: 13-35 portions of imported steam fish meal, 10-25 portions of imported direct-heat fish meal, 3-5 portions of maggot powder, 2-8 portions of corn protein powder, 5-10 portions of soybean meal, 2-8 portions of fermented soybean, 2-4 portions of soybean meal, 3-8 portions of cottonseed protein, 2-5 portions of peanut meal, 2-5 portions of colza meal, 3-8 portions of meat/bone meal, 2-5 portions of sleeve-fish visceral meal, 23-33 portions of high protein flour, 1.0-3.0 portions of soya bean lecithin, 2.0-5.0 portions of sea fish oil, 0.4-1.0 portion of choline chloride (50%), 0.1-0.3 portion of vitamin C-phosphate (35%), 0.5-1.5 portions of calcium biphosphate, 0.2-0.5 portion of compound multi-vitamin, 0.5-1.5 portions of composite mineral salt, 0.04-0.2 portion of lutein (20%), 0.1-0.3 portion of phagostimulant, 0.1-0.3 portion of micro-ecological preparation, 0.1-0.3 portion of beta-1,3 glucan, 0.01-0.04 portion of phytase and 0.04-0.08 portion of non-starch polyase. The feed can meet all the nutritional requirements of culturing the culter alburnus and the pelteobagrus fulvidraco, so that fish bodies can grow quickly, high feed conversion rate can be achieved, and the coefficient of the feed ranges from 1.2 to 1.4. By using the feed, the immunity performance and the anti-stimulation capability of the culter alburnus and the pelteobagrus fulvidraco can be obviously enhanced, the energy of the fish bodies can be ensured, and the survival rate is improved by 5-10%.

Methods and compositions are provided for increasing responsiveness to therapeutic metalloproteases including increasing and/or maximizing responsiveness and preventing botulinum and tetanus toxin resistance due to a functional deficiency of zinc. Also provided are methods for zinc replacement or supplement in lacking individuals comprising the administration of a zinc supplement for a loading period and/or administration of a phytase supplement together with the zinc supplement. Also provided are methods for standardization of botulinum toxin potency assays that provide for greater certainty and margins of safety in the use of products from different manufacturers.