71 results about "Phytase" patented technology

The invention provides a leavening agent and a preparation method thereof, and belongs to the technical field of leavening agents. According to the scheme, an efficient fermentation system is constructed through the synergistic effect of bacillus amyloliquefaciens, bacillus licheniformis, lactobacillus plantarum and saccharomyces cerevisiae, in the initial stage, the bacillus amyloliquefaciens secretes amylase and phytase degradation substrates, and conditions are created for the saccharomyces cerevisiae to metabolize monosaccharide to generate flavor substances and vitamins; in the middle stage, bacillus licheniformis decomposes protein to release amino acid, lactobacillus plantarum regulates pH through lactic acid metabolism to inhibit infectious microbes, and a multi-layer encapsulated compound enzyme preparation is introduced to synergistically degrade a complex substrate; in the later stage, fructooligosaccharide in the prebiotics-buffer stabilizer promotes proliferation of probiotics, mannitol and chitosan protect the activity of thalli and the function of metabolic enzymes, and the fermentation environment is stabilized; according to the whole system, the functionality, the environmental adaptability and the storage stability of the leavening agent are improved through flora synergistic metabolism, dynamic fermentation condition control and the action of the multifunctional additive, and the diversified requirements in industrial production are met.

The invention belongs to the technical field of food, and provides a special enzyme composition for infant soybean milk powder and soybean milk powder thereof. A three-stage directional enzymolysis technology is adopted, the three-stage directional enzymolysis technology comprises glutamine transaminase deamidation pretreatment, bacillus subtilis protease-trypsin-papain first-stage hydrolysis and aminopeptidase-phytase-beta-glucosidase second-stage hydrolysis, and conductivity control, carbon dioxide ventilation in-situ complexation and spray drying processes are combined. The free amino nitrogen of the product is increased by more than or equal to 20%, the proportion of 200-1500Da peptide fragments is more than or equal to 70%, the median particle size is 50-180 microns, the water activity is less than or equal to 0.30, the instant dissolving time is less than or equal to 30 seconds at 25 DEG C under the water hardness of 15-200ppm, the settling rate is less than or equal to 3% after standing for 30 minutes, and the zeta potential is-10 to-25mV. The palatability contradiction between deep hydrolysis and bitterness inhibition of the bean-based infant formula powder, the brewing contradiction between high-calcium reinforcement and instant stability and the process controllability contradiction between three-stage enzymolysis and ionic strength accumulation are effectively solved, and the bean-based infant formula powder has wide application value in the fields of bean-based infant formula food and vegetable protein nutritional products.

The present compositions and methods relate to thermostabilization of phytase with phosphate and increasing the recovery of phytase during the production of heat-treated food or animal feed pellets that contain phytase.

The invention discloses a phytase mutant AppA as well as a coding gene, a preparation method and application thereof. The phytase mutant AppA is obtained by mutating amino acids at the 137th site, the 185th site and the 255th site of wild type phytase AppA from asparagine, aspartic acid and tyrosine into valine, asparagine and aspartic acid respectively; the amino acid sequence and the nucleotide sequence of the gene are SEQ ID NO.1 and SEQ ID NO.2 respectively. The catalytic efficiency of the mutant enzyme AppA provided by the invention on sodium phytate is improved by 2.5 times compared with that of a wild enzyme AppA; under the condition of 70 DEG C, the half-life period of the mutant enzyme AppA is 3.4 times that of the wild enzyme AppA. The mutant enzyme AppA has the characteristic of high enzyme activity under a high-temperature condition, which indicates that the mutant enzyme AppA has an important application prospect in industrial production of a feed in which phytic acid needs to be degraded under the high-temperature condition.

The invention belongs to the technical field of agricultural animal husbandry application, and particularly relates to a feed additive for reducing backfat thickness of fattening Min pigs, a preparation method and application. The feed additive for reducing the backfat thickness of the fattening Min pigs comprises the following components in percentage by weight: 0.25 to 1.0 percent of hyodeoxycholic acid, 21.0 to 31.7 percent of citric acid, 0.7 to 3.2 percent of neutral phytase, 0.1 to 1.4 percent of astragalus membranaceus, 0.3 to 1.4 percent of saccharin sodium salt, 1.0 to 8.2 percent of rice hull powder and 54.0 to 64.7 percent of zeolite powder. The preparation method of the feed additive for reducing the backfat thickness of the fattening Min pigs comprises the following steps: crushing hyodeoxycholic acid, citric acid, neutral phytase, astragalus membranaceus, saccharin sodium salt, rice hull powder and zeolite powder into 80-mesh particles; the preparation method comprises the following steps: weighing the raw material components according to the ratio, stirring in a stainless steel mixer with the mixing uniformity of less than or equal to 5% for 5-10 minutes, and quantitatively packaging to obtain the finished product. The additive in the feed from the late stage of Min pig fattening to slaughtering can effectively reduce the backfat thickness of sows during slaughtering, improve the expression level of related genes of muscle fibers, and further improve the meat quality of Min pigs.

This document describes poultry diets containing phytase polypeptides or fragments thereof, in which the diet contains no or practically no inorganic phosphate.

The invention relates to the technical field of soybean meal processing, and discloses a soybean meal raw material treatment method, soybean meal and animal feed, the method comprises the following steps: (1) mixing a soybean meal raw material with a pretreatment reagent, and respectively carrying out first extraction, second extraction and solid-liquid separation to obtain a first solid phase; the pretreatment reagent comprises organic alcohol, citric acid, fructo-oligosaccharide and water; the temperature of the second extraction is 15-45 DEG C higher than that of the first extraction; (2) mixing the first solid phase with an enzyme preparation, performing enzymolysis, and performing solid-liquid separation on a product after enzymolysis to obtain a second solid phase; the enzyme preparation comprises phytase, cellulase and non-starch polysaccharide enzyme. By adopting the method disclosed by the invention, the content of antinutritional factors in the soybean meal raw material can be fully reduced, the protein content of the product is increased, the efficient utilization and low immunity characteristics of soybean protein are considered, and the enzymolysis soybean meal product with high protein, low antigen, low small peptide, low bad oligosaccharide, strong stability and good palatability can be obtained without excessive enzymolysis.

Provided is a parent phytase variant, which relates to the technical field of protein engineering. The variant, relative to the parent phytase thereof, has one or more amino acid substitutions at positions corresponding to positions 295, 349, and 374 of SEQ ID NO: 1. Compared to the parent phytase, the variant has increased thermal stability.

The invention provides a preparation method and application of biochar immobilized phytase, and relates to the technical field of biological enzyme immobilization. The method comprises the following steps: firing collected pineapple leaves into biochar for later use; adding pectin into water to obtain a pectin aqueous solution; adding the charcoal component into the pectin aqueous solution, uniformly mixing, filtering, washing and drying to obtain pectin charcoal; adding a cross-linking agent into the pectin biochar for cross-linking, then stirring and mixing with a phytase solution, filtering, and freeze-drying to obtain the biochar immobilized phytase. The biochar immobilized phytase can effectively overcome the defects that exogenous phytase is prone to inactivation and loss and difficult to store in soil, the phosphorus dissolving effect of phytase is enhanced, meanwhile, the biological activity and richness of indigenous phosphate-solubilizing bacteria are increased, soil phosphorus circulation is accelerated, soil phosphorus pool mineralization is promoted in the biochemical aspect, and the phosphorus-solubilizing effect is improved. The soil conditioner is a more environment-friendly and efficient soil conditioner and phosphate fertilizer synergist.

This invention discloses a fermentation method for improving the flavor and stability of old dough, belonging to the field of food technology. The method includes: mixing activated *Candida cruzi*, *Lactobacillus brevis*, *Hansenula polymorpha*, *Yarrowia lipolytica*, and *Saccharomyces cerevisiae* in a specific ratio to obtain a compound bacterial solution; and fermenting coarse grains with this compound bacterial solution to obtain coarse grain steamed buns. This invention employs the synergistic fermentation of five specific bacterial strains. *Lactobacillus brevis* and *Candida cruzi* synergistically optimize the rheological properties of the dough, *Hansenula polymorpha* and *Saccharomyces cerevisiae* synergistically produce rich aroma compounds, and *Yarrowia lipolytica* secretes phytase and cellolytic enzymes to lower the glycemic index. The coarse grain steamed buns prepared by this invention contain 55-65 flavor compounds, and the glycemic index is estimated to be reduced to 48-56, achieving a stable and controllable fermentation process, a low-GI product, and a rich flavor.

This invention discloses a light-aroma baijiu made from mung beans and its fermentation control method, belonging to the field of baijiu brewing technology. The invention uses 80-85% red sorghum and 15-20% mung beans as raw materials. After dehulling and crushing, the mung beans are liquefied with low-temperature α-amylase (30-60 U / g) and mixed with sorghum. The mixture is then treated with phytase (10-20 U / g), moistened at high temperature, and steamed to gelatinize. After cooling, light-aroma low-temperature koji and ester-producing yeast are added to strengthen the koji. The mixture undergoes a two-stage variable-temperature fermentation in earthen vats: the first 7-10 days at 18-22℃, and the subsequent 18-21 days gradually increasing the temperature to 25-28℃, with a peak temperature ≤32℃ throughout the process, for a period of 28 days. The fermented mash is mixed with rice husks, loaded into stills, and distilled over a low flame. The distillate temperature is ≤25℃. 5-8% of the first and last sips are discarded, and the 65% vol middle section of the liquor is collected and aged in earthenware jars for more than 6 months. This invention constructs a three-stage fusel oil regulation system: "low-temperature liquefaction to control precursors, variable-temperature fermentation to inhibit formation, and slow-fire distillation to retain." The finished wine has a fusel oil content of ≤0.8g / L, a natural mung bean aroma, and a high level of comfort after drinking.

The invention belongs to the technical field of gene engineering and enzyme engineering, and particularly relates to a high-temperature-resistant phytase mutant as well as a preparation method and application thereof. The 19 , 104 and 305 genes of a phytase gene appA are modified to obtain a plurality of high-temperature-resistant phytase mutants, the heat resistance of the three mutants G19P / G104P / D305R is remarkably superior to that of wild type and traditional phytase, experimental results show that after the phytase mutants are respectively treated for 20 minutes at the high temperature of 80 DEG C, 90 DEG C and 100 DEG C, the residual rates of enzyme activity are respectively 94.6%, 91.8% and 66.5%, and the residual rates of enzyme activity are respectively 94.6%, 91.8% and 66.5%. The phytase can completely tolerate granulation at the high temperature of 80-95 DEG C in the feed industry, and the industrial pain point of insufficient enzyme activity after traditional phytase granulation is greatly solved.

This invention discloses a high-efficiency protein expression system, its construction method, and its applications. The system includes a heterozygous promoter activator and a heterozygous promoter. The heterozygous promoter activator consists of the DNA-binding protein TetR, a linker peptide, and the transcription activator Mit1-Δ60-96. The heterozygous promoter consists of the protein-binding sequence TetO, a spacer sequence, and a basal promoter. The linker peptide is (GGGGS)×2, the spacer sequence is TCGCGCGC, and the basal promoter is P. AOX1 The core promoter of this invention. In the absence of a heterozygous promoter activator, the target protein gene initiated by the heterozygous promoter is expressed very weakly; in the presence of a heterozygous promoter activator, the target protein gene is expressed efficiently. This invention's system has good applicability and scalability, and the expression intensity of various target proteins, including xylanase, phytase, and glucosidase, is significantly higher than that of commonly used P. AOX1 Promoter.

The invention discloses a special feed for breeding laying hens and a preparation method thereof, and belongs to the technical field of livestock and poultry feeds. The special feed for breeding the laying hens consists of a basal feed and a core functional compound. The core function compound innovatively comprises an organic trace element compound composed of ferric glycine, zinc methionine and selenium yeast, an antioxidant and absorption enhancer composed of astaxanthin and medium chain triglyceride (MCT), and an intestinal health and calcium and phosphorus metabolism regulator composed of sodium butyrate, beta-glucan and phytase. The key point of the preparation method is that MCT is adopted to pre-mix and coat the core functional compound components. Through synergism of all the components, the egg laying performance, egg quality, oxidation resistance and bone health of laying hens are effectively improved, and safe, efficient and environment-friendly laying hen breeding is achieved.

The invention discloses a Chinese style functional pastry and a preparation method thereof. Firstly, white kidney beans are induced to germinate, endogenous phytase is activated to reduce the content of resistance factors such as phytic acid and lectin, and the activity of an alpha-amylase inhibitor is protected by spraying a soybean trypsin inhibitor. And then mixing the germinated white kidney beans with the aqueous extracts of astragalus membranaceus and poria cocos, inoculating saccharomycetes and lactobacillus plantarum for fermentation to generate flavor substances and active components, and consuming small molecular sugar to maintain the low GI characteristic. Finally, puerarin powder and the like are added into the white kidney bean paste and the Chinese yam paste to prepare dough, the dough wraps the functional stuffing prepared from the chia seed powder, the Chinese wolfberry fruit powder and the red date and longan paste, and the finished product is obtained through forming, steaming and rose dew incense. The method effectively solves the contradiction between alpha-amylase inhibitor loss and anti-nutritional factor degradation in germination, improves the bioavailability, flavor level and intestinal health regulation function of the product, and realizes the synergistic interaction of nutrition enrichment, blood sugar management and multiple nourishing regulation.

The invention relates to the technical field of livestock and poultry breeding feed, and discloses a breeding mixed feed additive formula and a feeding method. The additive is prepared from core nutrient components (calcium sulfate, methionine and lysine), immunity enhancing components (astragalus polysaccharide, eucommia ulmoides extract and probiotic compound preparation), digestion promoting components (amylase, phytase and fructo-oligosaccharide) and flavor improving components (pericarp residue compound) according to a specific weight part ratio. The feeding method combines physiological characteristics of 1-4 week old chicks, 5-16 week old bred chickens and laying hens / broiler chickens in the fattening stage, and the mixing proportion, the feeding amount and the feeding frequency are adjusted in a targeted mode. The technical defects that an existing feed additive is single in nutrition, poor in immunity enhancement effect, insufficient in meat quality improvement and unscientific in feeding are overcome, balanced nutrition supply, immunity enhancement, chicken flavor optimization and feed utilization rate improvement of chickens are achieved, and the chicken raising industry is promoted to develop towards the efficient, high-quality and green direction.

The application discloses a silage fermentation accelerator based on enzyme and bacteria cooperation and a preparation method thereof, and belongs to the technical field of feed preparation, and comprises the following steps: S1: taking sterilized selenium-rich wheat bran and sterilized yeast hydrolysate as carriers, mixing cellulase, xylanase, ferulic acid esterase, phytase and tannase to prepare a composite carrier enzyme; mixing composite bacteria powder and selenium-rich yeast powder to prepare an active mixture; mixing xylo-oligosaccharide, betaine and vitamin E to prepare active material to be embedded; S2: taking chitosan and sodium alginate as a skeleton, mixing N-isopropyl acrylamide, ammonium persulfate and N,N'-methylene bisacrylamide, and in-situ polymerizing to construct a temperature-sensitive hydrogel, and then performing dialysis, freeze-drying and grinding to prepare a temperature-sensitive embedding liquid; S3: rotating and shearing the active material to be embedded and the temperature-sensitive embedding liquid to prepare embedding suspension liquid to be sprayed, and then performing continuous spray embedding to prepare temperature-changing microcapsule particles, namely the silage fermentation accelerator based on enzyme and bacteria cooperation; and the application promotes silage fermentation.

This invention discloses a high-efficiency protein expression system, its construction method, and its applications. The system includes a heterozygous promoter activator and a heterozygous promoter. The heterozygous promoter activator consists of the DNA-binding protein TetR, a linker peptide, and the transcription activator Mit1-Δ60-96. The heterozygous promoter consists of the protein-binding sequence TetO, a spacer sequence, and a basal promoter. The linker peptide is (GGGGS)×2, the spacer sequence is TCGCGCGC, and the basal promoter is P. AOX1 The core promoter of this invention. In the absence of a heterozygous promoter activator, the target protein gene initiated by the heterozygous promoter is expressed very weakly; in the presence of a heterozygous promoter activator, the target protein gene is expressed efficiently. This invention's system has good applicability and scalability, and the expression intensity of various target proteins, including xylanase, phytase, and glucosidase, is significantly higher than that of commonly used P. AOX1 Promoter.

The invention relates to the technical field of food processing, and discloses a preparation process of fruit and vegetable extract compound fermented bean curd, which comprises the following steps: preparing a white blank inoculated with mucor sufu spores, and fermenting to obtain a blank; placing the blank in a vacuum environment, and dipping the blank in an enzymolysis modified fruit and vegetable penetrating fluid containing low-methoxyl pectin and phytase; the penetrating blank is subjected to sectional type vacuum breaking and re-pressing operation, the environment pressure is recovered to normal pressure in stages, penetrating fluid entering pores is subjected to in-situ gelation curing, and a cured blank is obtained; and salting the cured blank, bottling and carrying out later fermentation. The phytase and the low-methoxyl pectin are introduced into the penetrating fluid, the endogenous phytate of the blank is hydrolyzed by the phytase to release calcium ions, and the pectin is induced to be gelatinized and solidified in situ in the pores of the blank, so that liquid seepage caused by osmotic pressure difference in the subsequent processing process is blocked, and the retention rate of fruit and vegetable components in the finished product is increased.

The utility model discloses a system for preparing a tenebrio molitor feed formula based on fermented kitchen waste solid residues. The system comprises a waste sorting machine, a three-phase centrifugal machine, a stirring container and a special fermentation bag. The garbage sorting machine is connected with the three-phase centrifugal machine through a homogenizing device, the three-phase centrifugal machine is connected with the stirring container through a conveying device, and the special fermentation bag is arranged at the output end of the stirring container. Impurities of the kitchen waste can be removed firstly, then the sorted kitchen waste is fully homogenized and then heated, solid residues of the kitchen waste are obtained after centrifugal separation, then mixed strains such as saccharomycetes, lactic acid bacteria, bacillus subtilis and faecalis coccus are added into the solid residues of the kitchen waste, and the solid residues of the kitchen waste are mixed uniformly. In addition, according to the tenebrio molitor feed prepared by adding biological enzyme preparations such as cellulase, phytase, xylanase, protease and the like, the quality of kitchen three-phase solid residues can be guaranteed for more than half a year by controlling fermentation conditions, so that the fresh-keeping effect is improved.

The invention provides a high-stability D-biotin feed additive as well as a preparation method and application of the high-stability D-biotin feed additive. The high-stability D-biotin feed additive is prepared from the following raw materials: 1 to 3 parts of vitamin E, 1.5 to 3.0 parts of vitamin C, 2 to 4 parts of L-arginine, 0.5 to 1.2 parts of glutathione, 1.0 to 2.5 parts of zinc methionine, 0.1 to 0.5 part of selenium yeast, 0.5 to 1.5 parts of high-temperature-resistant phytase, 15 to 25 parts of a mesoporous silica-montmorillonite composite carrier, 8 to 15 parts of pH response type polymer microspheres and 5 to 10 parts of modified starch. The pH response type polymer microspheres are targeted microspheres loaded with D-biotin. The utilization rate and nutrition supply efficiency of active ingredients in the additive are improved, and finally the feeding effect of the feed is improved.

Provided are a phytase mutant and a coding DNA molecule thereof, a vector, and a host cell. The phytase mutant comprises an amino acid sequence having at least 90% identity with SEQ ID NO: 3, and compared with SEQ ID NO: 3, and contains an amino acid substitution at at least one position selected from the group consisting of 36, 126, 211, 253, 258, and 266. The heat resistance of the mutant is significantly improved, thus facilitating the wide application of phytase in feed.

This invention belongs to the technical field of laying hen feed additives, specifically relating to a selenium-enriched, high-DHA, low-cholesterol functional egg feed additive and its preparation method. The additive is prepared by compounding selenized ginseng polysaccharide, Schizochytrium algae powder, rosemary extract, natural vitamin E, EGCG green tea extract, phytosterol micron powder, sodium butyrate, β-glucan, phytase, modified attapulgite clay, and maltodextrin in specific weight parts, followed by low-temperature premixing activation, antioxidant encapsulation treatment, low-temperature compound granulation, sieving, and packaging processes. This invention, through the synergistic effect of its components, enables eggs to reach stable functional indicators within 14 days of feeding. The yolk selenium content reaches 32.1~38.2 μg / 100g, DHA content reaches 210.5~261.7 mg / 100g, and cholesterol reduction rate reaches 16.7%~22.3%. Simultaneously, eggshell strength increases by 12.1%~18.5%, egg production rate remains at 95.1%~96.8%, and egg breakage rate decreases by 59.0%~72.0%. The product exhibits good stability, requires low dosage, and is easy to use, making it perfectly suited for large-scale egg-laying hen farming scenarios and possessing outstanding economic and health value.

The invention discloses application of biological malic acid and a mixture thereof in improving the production performance of laying hens. The laying hens are hyline brown laying hens; the mixture comprises biological malic acid and a carrier in a mass ratio of (1-2): (2-1), and the carrier comprises montmorillonite and zeolite powder; the feed is prepared from the mixture and basic feed, the mass ratio of the mixture to the basic feed is 1.2: 98.8, and the basic feed comprises the following components in parts by mass: 62.32 parts of corn, 25.68 parts of 43% soybean meal, 9.18 parts of mountain flour, 0.18 part of DL-methionine, 0.90 part of calcium hydrophosphate, 0.30 part of table salt, 0.02 part of phytase and 0.12 part of premix; the feed is used for feeding laying hens when the laying hens are in an egg-laying peak period. The production performance, egg quality and body health of laying hens can be improved by using biological malic acid.

The application relates to the technical field of genetic engineering and protein engineering, and particularly provides a high-temperature-resistant neutral phytase mutant and application thereof. The heat resistance of the mutant is significantly enhanced. The phytase mutant containing a V405P single-point mutation has the strongest heat resistance, and the enzyme activity residual rate is as high as 88.68% after being treated at 90 DEG C for 5 min, unexpected technical effects are achieved. The mutant can be widely applied to aquatic feed.

The application relates to a production method and application of combined fermentation of bupleurum and lonicera japonica residues by bacillus velezensis and pediococcus acidilactici, and belongs to the technical field of biotechnology. The bupleurum and lonicera japonica residues are treated by adopting a process of enzymolysis first and then fermentation, wherein the composite enzyme comprises cellulase, beta-glucanase, xylanase, beta-mannanase and phytase; and the fermentation bacteria comprise bacillus velezensis and pediococcus acidilactici. The fermented bupleurum and lonicera japonica residues provided by the application have a high release amount of active ingredients (chlorogenic acid, forsythoside and baicalin), and the addition ratio in broiler chickens is 2.0%-4.0%. The application effect includes improving growth performance, improving the levels of anti-inflammatory factor IL-10 and serum immunoglobulin IgM of the body, improving the muscle quality and flavor characteristics of the breast muscle, and improving the contents of inosinic acid, intramuscular protein, flavor amino acid and total amino acid. The product can not only improve the yield and quality of livestock products, but also relieve the pressure on the environment caused by solid waste residues, and has a good market prospect.

This invention relates to a method for improving the intestinal tolerance of wheat bran dietary fiber based on synergistic modification by microorganisms and enzymes. The method includes ultra-fine grinding and high-pressure moist heat activation of wheat bran; multi-enzyme synergistic microbial stepwise treatment, first using high-temperature amylase and neutral protease to remove interfering components such as starch and protein, then using laccase to degrade lignin, resulting in a looser structure; subsequently, phytase efficiently degrades phytic acid, Aspergillus nidulan degrades the side chains of arabinogalactan, and snail enzyme and ferulic acid esterase work synergistically to convert insoluble arabinoxylan into soluble dietary fiber and release phenolic antioxidants. This method increases the soluble dietary fiber content of wheat bran by 2 times, reduces phytic acid to 10 mg / g, increases free phenolic acid content by 40%, achieves a DPPH free radical scavenging rate of 60%, significantly enhances antioxidant activity, and significantly reduces IgG levels in mouse models compared to those consuming unmodified wheat bran, thus improving intestinal tolerance and probiotic function; the product can be used as a high-quality functional food ingredient.

The application discloses two purple acid phytase genes of alfalfa which can enhance the aluminum toxicity stress tolerance of plants SgPAPhy_a and SgPAPhy_b and application thereof, and transgenic plants of the genes SgPAPhy_a and SgPAPhy_b can significantly improve the aluminum toxicity stress tolerance of plants; SgPAPhy_a The genes and SgPAPhy_b have important effects on improving the aluminum toxicity stress tolerance of plants, can be used for improving crops through transgenic technology, and improve the aluminum toxicity stress tolerance of plants, and have important guiding significance for subsequent cultivation of new germplasm of aluminum toxicity stress tolerant crops suitable for planting in acid soil.

The invention discloses a feeding method for breeding sows with different birth times, a feed additive for breeding sows with low birth times and biological feed of the feed additive, and relates to the technical field of livestock breeding feed. The invention provides different feeding matching schemes for the reproductive sows with different birth times: low-birth-time high-crude fiber daily ration + NCG and high-birth-time low-crude fiber daily ration; the feed additive for the low-fetal-rate reproductive sows is prepared from the following components in parts by weight: 0.1-1.0 part of N-carbamylglutamic acid, 10-150 parts of soybean hulls and 10-100 parts of beet pulp. The biological feed comprises the additive and a basal feed, and is also added with specific doses of vitamins, minerals, enzyme preparations (phytase, beta-glucanase and the like) and bacillus licheniformis, the invention systematically reveals the differentiated influence of the daily ration fiber level and NCG on the reproductive performance of low-fetal-count (1-5-fetal-count) and high-fetal-count (6-8-fetal-count) sows for the first time; a traditional one-step feeding mode is broken through, and a personalized nutrition scheme is provided.

The invention provides a fish meal substitute as well as a preparation method and application thereof. The preparation method comprises the following steps: S1) mixing 0.1-3 parts by weight of a fermentation inoculant, 0.1-4 parts by weight of a compound enzyme preparation and water to obtain a seed solution; mixing the raw materials to obtain a raw material mixture; s2) mixing the seed solution and the raw material mixture, and performing fermentation treatment to obtain the fish meal substitute, wherein the fermentation inoculant comprises saccharomycetes and / or bacillus; the compound enzyme preparation comprises protease and phytase; the raw materials comprise one or more of yeast protein, vegetable protein, mineral elements or amino acid. The fish meal substitute can solve the problem of insufficient nutrition of fish meal substitutes in the prior art and is suitable for the field of feed preparation.