344 results about "Dismutase" patented technology

The invention discloses a platinum nano-material with the activity of four mimic enzymes and a preparation method thereof. The preparation method comprises the following steps: using citrate ions as a modifier, reducing chloroplatinic acid by utilizing sodium borohydride, and preparing the platinum nano-material modified by citric acid. The platinum nano-material is simply and quickly prepared, the particle size is small, and the dispersibility and stability of a water solution are high. The obtained platinum nano-material has the activity of four mimic enzymes at the same time, and comprises peroxidase, oxidase, catalase and superoxide dismutase. The activity of the four mimic enzymes of the platinum nano-material can be adjusted and controlled through the concentration of the citrate ions used in the preparation process.

The invention provides a SOD gene from animal and a method for cloning and expressing the SOD gene in colibacillus and yeast cells, wherein a nucleotide sequence of superoxide dismutase is shown as SEQ NO.1; an amino acid sequence of the superoxide dismutase is shown as SEQ NO.2; carriers of nucleotide molecules are colibacillus plasmids or yeast plasmids; cells of the nucleotide molecules are formed by carrier conversion; and cells of the nucleotide molecules of the superoxide dismutase contain colibacillus containing the nucleotide molecules or converted by the carriers or pichia yeast containing the nucleotide molecules or converted by the carriers. The invention can prepare recombinant production strains which can efficiently express and secrete Cu/Zn-SOD, realizes the production industrialization of the Cu/Zn-SOD, and achieves good pH stability, favorable thermal stability and Cu/Zn-SOD products with anti-protease hydrolyzation capacity.

Methods and pharmaceuticals for inhibiting or preventing metastasis formation in animals, including humans, having primary tumors, through the administration of phosphorothioates including their thiol and disulfide metabolites are disclosed. These compounds stimulate angiostatin levels, inhibit matrix metalloproteinases (MMPs), and stimulate manganese superoxidase dismutase (MnSOD). Phosphorothioates, of which amifostine is an example, can be administered as a combination therapy with traditional cancer therapies, including chemotherapy, radiotherapy, surgery, immunotherapy, hormone therapy and gene-therapy. Inhibition or prevention of metastasis by phosphorothioates is independent of tumor type, including adenocarcinomas and sarcomas.

The invention relates to a termite pheromone attractant and a termite trap-killing agent using the attractant, and belongs to the technical field of termite trap-killing agents. The termite pheromone attractant comprises the following components: hydroquinone, arbutin, oleic acid, glucose, cyclohexanhexol, mannose, 2,6-di-tert-butyl paracresol, amino acid, a biological active enzyme, salt, codlemone, gloeophyllum trabeum, ethanol, 2-phenoxyethanol, n-hexane, dismutase, poria cocos, coriolus versicolor, tall gastrodia tuber, edible fungus, lucid ganoderma, bee honey, maltose, fermented sugarcane powder, fermented straws and acetone. The termite pheromone attractant disclosed by the invention synchronously has the functions of a termite phagostimulating pheromone and a termite trace pheromone by adding a chemical pheromone into a termite trap-killing agent, and can be used for significantly increasing the eating amount of termites on the trap-killing agent and improving the trap-killing effects of the termite trap-killing agent; and meanwhile, the termite pheromone attractant has extreme sensitivity and high specificity.

The invention discloses a method detecting activity of plant superoxide dismutase, catalase and peroxidase, which comprises the steps of grinding a sample by using a liquid nitrogen method, extracting three enzymes simultaneously by adopting one buffer solution, detecting enzyme activity and the like. In the method, SOD, CAT and POD in plants can be simultaneously extracted by adopting one buffer solution, so that the method fulfills the aim of simply and quickly detecting the enzyme activity of the SOD, CAT and POD, simplifies operation, saves time and labor, reduces the using amount of samples and reagent and the like, and contributes to the realization of batch operation, and the detection result is similar to that of the conventional method. Furthermore, the sample is ground by adopting a liquid nitrogen grinding method before enzyme solution is extracted, so that the method has the advantages of high grinding speed, good cell crushing effect and high extraction efficiency.

The invention discloses a process for adding superoxide dismutase (SOD) in fruit wine making. By adding the SOD into various of fruit wine, free radicals in body can be cleared once. The process comprises the following steps: (1) culturing yeast strains at 30+/-1 DEG C for 24-30 hours, and inoculating 0.1-1% of the vigorous strains growing for a logarithmic phase into a fermentation vat; (2) strictly dividing the fermentation into primary fermentation of 4-7 days and secondary fermentation of 15-25 days before entering an ageing phase, wherein the sugar control standard in fermentation is 14%; (3) strictly testing the active unit of the SOD not lower than 6,000u/L by a pyrogallol auto-oxidation macro-method in a blending and self-inspection process; and (4) adding the SOD into the fruit wine. By adopting the process disclosed by the invention, the prepared fruit wine can clear redundant free radicals so that the free radicals reach a dynamic balance in body and the diseases caused by cellular aging are prevented (slowed down).

The invention discloses a method for improving the fermentation productivity of carotenoid, which is realized by the following steps: rhodotorula mucilaginosa is used as fermentation strains of the carotenoid, and the rhodotorula mucilaginosa is inoculated into a culture medium according to the inoculum size of 2%-10%; a ferrous ion solvent is added into the culture medium, wherein the volume of addition of the ferrous ion solvent is that the molarity relative to the culture medium is between 0.05-0.5mmol/L, and the fermentation temperature is kept at 27-33 DEG C; after the fermentation lasts for 32-40 hours, the temperature is lowered to 20-25 DEG C for continuous fermentation; the fermentation lasts for 24-46 hours. By adding ferrous ions, the capability of beta-carotene for converting into the high antioxygen carotenoid is greatly improved, the insufficience of quantity of beta-carotene dioxygen dismutase and beta-carotene hydrogenase is made up; the beta-carotene is converted into the high antioxygen carotenoid; by the method, the fermentation yield is improved by 30-34%.

The invention provides a Fe3O4 nanozyme with improved catalytic activity, and a preparation method and application thereof. By doping metal elements in Fe3O4 nanozyme, the dismutase activity of peroxidase, catalase and/or superoxide can be improved; the metal element doped Fe3O4 nanozyme can be prepared in large quantities, has low cost, good stability at room temperature, good safety, and good economic value and application prospect.

The invention discloses a cultivation method for a fruit tree bearing fruit rich in superoxide dismutase. The cultivation method is a planting method which employs one or more microbial preparations to produce fruit rich in copper-zinc superoxide dismutase (Cu/Zn-SOD). Compared with the prior art, the cultivation method provided by the invention has the advantage that the content of SOD is greatly increased. By eating the fruit, the health conditions of people can be greatly improved; elimination of sub-health can be facilitated; immunity can be enhanced; excess superoxide anion free radicals in human bodies can be scavenged; and occurrence of a plurality of diseases can be prevented.

The invention provides a coproduction process for extracting various bioactivators from pig placenta, which comprises the following steps: taking the pig placenta (containing farrowing blood) as a raw material, utilizing components rich in the raw material such as immune globulin (Ig), immunocompetent peptide, superoxide dismutase (SOD), lipopolysaccharide, placental protein and the like, and applying modern biochemistry technology and biological integration technology such as salting in, salting out, chromatography, ultra-filtration and the like according to different structures, molecular weights, isoelectric points, physical and chemical properties, biological activities of the components to make the coproduction process suitable for industrial production. Therefore, in the same flow of the production process, the effective components in the pig placenta with market development value can be produced, namely five products of the immune globulin, the immunocompetent peptide, the superoxide dismutase, the lipopolysaccharide and the placental protein. The process combines the conventional and modern processes, produces various products through coproduction, and not only makes full use of placenta resources to change the waste into the treasure, does not discharge waste liquid and prevents the environment, but also can greatly reduce the production cost and improve the economic benefit.

The invention provides a method for cultivating wheat seedlings by using nitrogen stress nutrient solution. The method comprises the following steps of: repeatedly soaking wheat seeds in nitrogen stress solution until the wheat seeds sprout and grow to the height of between 5 and 20cm; and accumulating active oxygen in cells of the wheat seedlings by nitrogen stress to increase the quantity and activity of protective enzyme and enrich a polyphenolic substance. In order to prevent excessive active oxygen from damaging the cells, a method for alternating repeated and short-term nitrogen stress and nutrient solution cultivation is adopted to make the active oxygen slowly generated. By the method, more superoxide dismutase, catalase, peroxidase and the polyphenolic substance can be obtained, the yield of the wheat seedlings is improved, and new species of wheat seedlings rich in antioxidants are produced.

The invention discloses an anti-oxidation active extract of citrus peels as well as an extraction process and application thereof. The active extract of the citrus peels mainly contains a citrus flavonoid substance and pectic polysaccharide. The extraction process of the active extract of the citrus peels comprises the steps of preprocessing raw materials, refluxing and extracting by a dipping method, decompressing and concentrating and drying in vacuum. The invention takes the in-vitro oxidation resistance of the extract to combine with the extraction yield as indexes, adopts a response surface method to optimize the extraction process, has reasonable technological conditions and good effect stability and is suitable for industrialized production. The active extract of the citrus peels, which is obtained by extraction, has an obvious effect to a model mouse with senility caused by D-galactose and can lower the content of the MDA (malondialdehyde) of the plasma, the liver and the brain obviously; the activity of the SOD (superoxide dismutase) of the plasma, the liver and the brain is obviously enhanced; the activity of the GSH-Px (glutathione peroxidase) of the plasma and the liver is obviously enhanced; and the invention can be used for preparing an anti-oxidation medicine, an anti-oxidation food or an anti-oxidation health-care product.

A processed food excellent for activating superoxidase dismutase is disclosed. The food contains 30 to 70% by weight of barley, wheat, rye or oats; 1 to 50% by weight of water soluble dietary fibers; 1 to 10% by weight of oligosaccharides; 0.5 to 5% by weight of powdered lactic acid bacteria and 5 to 15% by weight of powdered green tea. A method of making the food and a method for activating superoxide dismutase using the food is also disclosed.

Provided are novel human copper-zinc superoxide dismutase, also known as superoxide dismutase 1 or SOD1, specific antibodies as well as fragments, derivatives and variants thereof as well as methods related thereto. Assays, kits, and solid supports related to antibodies specific for SOD1 are also disclosed. The antibody, immunoglobulin chain(s), as well as binding fragments, derivatives and variants thereof can be used in pharmaceutical and diagnostic compositions for SOD1 targeted immunotherapy and diagnosis, respectively.

The invention belongs to the field of medicament synthesis, and relates to Danshensu derivatives with a structure in the following formula, a synthesis method thereof, and application thereof in pharmacy. The synthesis method adopts a chiral synthesis method to synthesize the Danshensu derivatives with stable structures in high yield, in particular derivatives of dextrorotatory Danshensu with high optical purity. Through experiments of animal models with hypoxic myocardial cells, results show that the Danshensu derivatives can reduce LDH leakage of the hypoxic myocardial cells, improve the activity of hyperoxide dismutase in the cells, inhibit generation of a product of lipid peroxidation, promote the expression of anti-apoptotic gene bcl-2 mRNA and inhibit the expression of pro-apoptosisgene bax mRNA and apoptotic gene caspase-3mRNA; and the results prove that the Danshensu derivatives have an effect of protecting hypoxic cardiac muscle and can be used for preparing medicaments for treating ischemic heart disease.

The invention discloses application of remedied domestic waste composts in adjusting the activities of antioxidant enzymes of lawn plants; wherein, the remedied waste composts refer to compost matrixes obtained by eluting the composts with water based on the weight ratio of 1:1-3; the activities of the antioxidant enzymes comprise superoxide dismutase, peroxide dismutase and catalase. The technical effects of the invention show that the method of planting lawn plants on the remedied waste composts not only can enhance the activities of the protective enzyme systems of the lawn plants but also can improve the capabilities of the lawn plants in resisting unfavorable growth environment obviously, therefore, the technology not only finds a way for scientifically and effectively planting environment-friendly lawns on the matrixes of the eluted compost, but also can achieve the purpose of reducing the environment pollution caused by the domestic waste composts and provide technical support for applying the remedied waste composts to the lawn planting system.

The invention discloses preparation and application of an Mxene-Mn3(PO4)2 composite electrode material. The preparation is characterized in that two-dimensional Ti3C2 nanosheets are synthesized from aTi3AlC2 ceramic material as a raw material, and ATP (adenosine triphosphate) is used as a template to induce synthesis of Mn3(PO4)2 nanoparticles. The Ti3C2/Mn3(PO4)2 nanocomposite synthesized with the method integrates good conductivity of Ti3C2 and biological mimetic enzyme activity of Mn3(PO4)2, and the composite material has excellent conductivity and catalytic performance by complementary advantages of the two raw materials, and is the excellent electrode material for preparing superoxide dismutase. The composite material can be used for constructing biosensors and has excellent application prospects in the fields of medical diagnosis, medical research and the like.

The present invention discloses a blueberry enzyme and a preparation method thereof, which belongs to the field of enzyme preparation. The preparation method comprises: (1) blueberries are washed and cleaned, hot air dried at a temperature of 50-60 DEG C, and reserved for the next step; (2) black sugar is disinfected with ultraviolet for 5-10s; (3) the obtained blueberries in the step 1 are put into a sterile reactor, the obtained black sugar in the step 2 is added into the blueberries at a 17.5-18.5% mass of the blueberries, and a stabilizer is added into the blueberries with a mass being 0.5-1.4% that of the blueberries to obtain a mixture; (4) yeasts are added into the mixture obtained in the step 3; and (5) the mixture is sealed and fermented. The addition amount of sugar in the blueberry enzyme is low, which avoids a large amount of sugar content during drinking the enzyme. The blueberry enzyme is short in reaction time, anthocyanins and superoxide dismutase ingredients are retained, and total phenolic content is high.

The invention discloses a technology for extracting proteins and superoxide dismutase (SOD) from horseradish tree leaves. The technology comprises the following steps: smashing and pulping fresh horseradish tree leaves, and performing digestion, pressure filtering, isoelectric precipitation and centrifugation to obtain precipitates and supernate; performing modification protection, thermal denaturation reaction and freeze drying on the precipitates to obtain SOD freeze-dry powder; performing isoelectric precipitation, centrifugation and spray drying on the supernage to obtain leaf protein powder. By the adoption of the technical methods such as normal-temperature digestion, isoelectric precipitation, centrifugal separation, the original functional characteristics of the proteins and the activity of enzyme are retained to the maximum extent, and the yield of the proteins and that of active enzyme are greatly increased; the obtained leaf protein powder is bright in color, high in protein content and stable in quality; the obtained SOD is high in activity, and the practical value and the economical benefit are higher. The technology is easy to operate, low in production cost, safe, reliable and suitable for industrial production.

The invention relates to a process for producing wines, in particular to a method for producing a dismutase loquat wine. The method for producing the dismutase loquat wine is characterized by comprising the following steps: A) selecting fresh and ripe fruits, sterilizing the peel by utilizing a 1/5000 potassium hypermanganate solution, washing with clean water, artificially removing the kernels, juicing by utilizing a juicer, and transferring the juice into a fermentation jar; B) forming sufficient oxygen space because the juice accounts for about 1/3-1/2 of the volume of the jar after being transferred into the jar, adding a 3-11% white sugar solution dissolved in advance to be uniformly stirred, and then, inoculating a fresh Sacch Cervisiae culture solution, wherein the inoculation amount is 0.01-1%; storing the mixture for not more than 24h, and culturing for 96-168h at 33+/-3 DEG C so that the mixture is abundantly multiplied under the aerobic condition; and when the amount of live bacteria reaches more than 100 millions/ml, fermenting after executing. The dismutase can be matched with the loquat wine to produce the dismutase loquat wine which has good flavor, is beneficial to the human body and has the advantages of fragment wine flavor and sour, sweet and delicious taste.

The invention provides an antioxidative animal bifidobacterium strain with the preservation number is CCTCC (China Center For Type Culture Collection) NO:M2013282. The animal bifidobacterium can be used for preparing pharmaceutics, food or health-care products. With the animal bifidobacterium, the enzymatic activity of superoxide dismutase, glutathione peroxidase and catalase in an organism can be obviously improved, the level of malondialdehyde in the organism can be lowered, so that the antioxidation capability of the organism can be effectively improved, the immunity of the organism can be enhanced, and the antioxidative animal bifidobacterium can be widely applied in pharmaceutics, food or health-care products.

The invention provides a preparation method of an antioxidant peptide component and applications of the antioxidant peptide component in oral liquid preparations. The process conditions for preparinga forest frog oil antioxidant peptide component by pepsin/trypsin two-step hydrolysis mode are as follows: pepsin content is 2.0%, pepsin enzymolysis time is 3.5 h, trypsin content is 2.0%, and trypsin enzymolysis time is 3.0 h. Under the conditions, the forest frog oil antioxidant peptide component can remove 75.57% of hydroxyl free radicals, and has a yield of 63.45%. An ultrafiltration system intercepts the peptide component with the molecular weight of 1-3kDa so as to obtain the forest frog oil antioxidant peptide component IV with the highest activity. The peptide component has remarkableantioxidant effect in ethanol-induced oxidative damage model mouse body, can reduce the content of malondialdehyde and protein carbonyl, can increase the activity of total superoxide dismutase and the content of glutathione, and can be used for preparing a forest frog oil antioxidant peptide component oral liquid.