47 results about "Dismutase" patented technology

The application discloses a superoxide dismutase biological anti-stress nutrient fertilizer based on plant extraction and a production method thereof. The fertilizer contains, in percentage by mass, 1.0-10.0% of superoxide dismutase, 1.0-5.0% of a plant anti-stress inducing factor complex, 0.1-2.0% of organic chelated trace elements, 5.0-15.0% of a phosphorus-potassium enhancing component, 0.5-5.0% of a natural protective agent, 3.0-10.0% of a penetrating agent, 0.5-15.0% of a microbial metabolic synergist, 0.1-5.0% of a pH buffer component, 0.01-0.5% of a plant-derived preservative, and the balance of water. The superoxide dismutase is preferably derived from corn germ or spinach extract. The production method comprises the steps of enzyme activity component preparation, water phase base preparation, anti-stress function phase preparation, step-by-step mixing and primary stabilization, final product integration and secondary stabilization, and the like. Through scientific compounding and synergy of various biological active components, the application significantly enhances the resistance of crops to drought, saline-alkali, high and low temperature and the like, and promotes growth and quality improvement.

This disclosure relates to compositions and methods using genetically modified bacteria that express superoxide dismutase (SOD) and catalase enzymes to reduce oxidative stress in the skin, and can be used for various skin conditions. In some embodiments of this disclosure, the genetically modified bacteria are Gram-positive bacteria, for example, of the genus Corynebacterium.

This invention relates to the field of bioengineering technology and provides an application of the maize heat shock transcription factor gene ZmHSF18, wherein the nucleotide sequence of the maize heat shock transcription factor gene ZmHSF18 is shown in SEQ ID NO:1. Experiments have demonstrated that overexpression of the maize heat shock transcription factor gene ZmHSF18 in plants can significantly improve seed germination rate and frozen survival rate at the mature stage under low-temperature stress, reduce malondialdehyde accumulation, and increase superoxide dismutase activity. Therefore, this maize heat shock transcription factor gene ZmHSF18 can be used to improve plant cold tolerance and create transgenic plants with enhanced cold tolerance, possessing important application value in plant genetic engineering and stress resistance breeding.

This invention discloses the application of the blueberry VcERF70 gene in improving cadmium tolerance in plants, belonging to the field of plant genetic engineering technology. The amino acid sequence of the VcERF70 protein is shown in SEQ ID NO.2, and the nucleotide sequence of its encoding gene is shown in SEQ ID NO.1. This invention constructs a plant overexpression vector containing the VcERF70 gene and genetically transforms it into blueberries. The resulting transgenic plants exhibit significantly reduced malondialdehyde and hydrogen peroxide content under cadmium stress, and significantly increased superoxide dismutase, catalase, peroxidase activities, and glutathione content. Experiments demonstrate that overexpression of the VcERF70 gene can effectively enhance the antioxidant defense capacity of blueberries, reduce cadmium stress-induced oxidative damage, and thus improve the blueberry's tolerance to cadmium. This invention provides a new gene resource and technical pathway for blueberry stress-resistant breeding.

The application relates to a composition for treating reflux esophagitis and application thereof. The composition comprises the following components in parts by weight: 2-8 parts of scutellaria baicalensis, 3-7 parts of licorice, 5-10 parts of beeswax and 80-120 parts of vegetable oil. The composition for treating reflux esophagitis contains scutellaria baicalensis, licorice, beeswax and vegetable oil, and through mutual cooperation among the components, the drug prepared from the composition can significantly increase the plasma motilin level of a patient, further increase the pressure of the lower esophageal sphincter, promote gastric emptying and prevent acid reflux, promote the recovery of reflux esophagitis, the esophagus of the patient is obviously improved, and no adverse reaction is found; meanwhile, the content of lipid peroxide can be obviously reduced, the activities of superoxide dismutase and glutathione peroxidase are increased, the esophageal mucosa is protected and treated, the pathological changes of the esophagus are obviously improved, and the treatment effect is excellent.

This invention belongs to the field of ecological environment monitoring technology and relates to a multi-level bioindicator method for the effective phytotoxicity of heavy metal-like substances / heavy metals. The method includes the following steps: S1. Obtaining plant samples affected by the soil or water body to be tested; S2. Measuring the activity of superoxide dismutase (SOD), gibberellin (GA), and chlorophyll content in the plant samples; S3. Based on the measured results of SOD activity, plant hormone content, and chlorophyll content, and their changes over time, classifying and assessing the effective phytotoxicity effects of heavy metal-like substances or heavy metals. This invention is the first to propose a unified three-level bioindicator method (SOD activity, GA hormone, and chlorophyll) that can effectively reflect soil pollution levels for typical heavy metal-like substances / heavy metals present in the actual environment, enabling a simpler assessment of the degree of heavy metal pollution in actual soil.

This application belongs to the field of microbial technology, and mainly relates to a strain of *Pediococcus lactis* BPA03 and its applications. *Pediococcus lactis* BPA03 is classified and named *Pediococcus lactis*. Pediococcus acidilactici This *Pediococcus lactis* strain BPA03 was deposited on September 28, 2025, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 36109. It inhibits skin melanin deposition and improves UV photoaging, suppresses the gene expression levels of immune factors such as IL-1β, IL-6, IL-8, and TNF-α, improves the activities of superoxide dismutase, catalase, and glutathione peroxidase, inhibits vinorelbine-induced neutropenia, and improves the inhibition of immune factor expression induced by vinorelbine, thus exhibiting comprehensive anti-aging effects.

This invention relates to the field of plant disease biological control technology, and particularly to a Fusarium oxysporum cell wall extract, its preparation method, and its application. The extract is derived from the polysaccharide fraction of the cell wall of Fusarium oxysporum spores or hyphae, and contains at least one pathogen-associated molecular pattern (PAMP) component. This component is a non-protein, thermally stable, species-nonspecific elicitor capable of forming an aqueous dispersion system. The extract of this invention can be recognized by plant PRRs and activate PTI immunity. Rehmannia glutinosa tubers treated with the extract showed a significantly increased germination rate after inoculation with Fusarium oxysporum. It also induces superoxide anion production, upregulates the activities of superoxide dismutase, peroxidase, and catalase, and enriches differentially expressed genes in the MAPK signaling pathway, jasmonic acid metabolism pathway, and phenylpropane biosynthesis pathway. Furthermore, the extract can directly inhibit Fusarium oxysporum colony expansion, conidia production, and mycelial growth. Extracts derived from spores or hyphae show no significant difference in inducing disease resistance, and the raw materials are flexible and easy to industrialize. This extract is derived from the cell wall of the pathogen itself, making it environmentally friendly and free from the risk of chemical pesticide residues. It is suitable for the green control of root rot disease in Rehmannia glutinosa.

The application discloses an application of near-infrared controllable Fe, Zn doped tea polyphenol based carbon dot nanometer enzyme in fruit preservation, and belongs to the technical field of agricultural product preservation.The application takes tea polyphenol as a precursor, and prepares Fe, Zn doped carbon dots (Fe, Zn@GTP) nanometer enzyme with excellent antioxidant and antibacterial activity through a microwave assisted method.The Fe, Zn@GTP nanometer enzyme exhibits near-infrared light enhanced peroxidase, superoxide dismutase and catalase catalytic activity and photo-thermal performance.The Fe, Zn@GTP has antibacterial effects on escherichia coli, staphylococcus aureus, methicillin-resistant staphylococcus aureus, ampicillin-resistant escherichia coli and penicillium expansum, and the inhibition rate of the bacterial biofilm treated by the Fe, Zn@GTP is more than 90%.In the preservation experiment, the PVA / Fe, Zn@GTP film can effectively prolong the preservation period of fruits, significantly reduce the occurrence rate of fruit mold and browning, and the deterioration degree of quality indexes.

The present application provides a composition with satiety and weight control and anti-aging, and a preparation method and application thereof. The composition comprises the following raw materials by weight: polysaccharide 11-13 parts, fiber powder 20-25 parts, highland barley powder 12-18 parts, compound powder 18-23 parts, pectin 4-8 parts, ganoderma lucidum powder 6-9 parts, and compound protein 8-12 parts, and relates to the technical field of satiety and weight control and anti-aging compositions. The composition can form an effect of hindering nutrient absorption or prolonging gastric emptying, increase the volume of intestinal contents to achieve satiety, thereby achieving the functions of well controlling weight gain and reducing body weight. In addition, the composition can activate the body's antioxidant enzyme system, improve the activity of superoxide dismutase (SOD), and reduce the content of malondialdehyde (MDA), thereby delaying cell aging and achieving the anti-aging effect.

The application provides a temperature-sensitive antibacterial and antioxidant gel, and a recombinant fusion protein sequence with antibacterial and antioxidant activity used in the gel is recombined by 1 human-derived superoxide dismutase (hSOD1), 1 rigid dipeptide and 1 endolysin (TSPpgh) sequence, wherein the human-derived superoxide dismutase (hSOD1) sequence is connected with the endolysin (TSPpgh) sequence through the rigid dipeptide (LP) to facilitate the respective protein activity, then the recombined fusion protein is used as antibacterial and antioxidant raw material, mixed with a temperature-sensitive chitosan hydrogel base, and finally prepared into the temperature-sensitive antibacterial and antioxidant hydrogel. The composite temperature-sensitive hydrogel has multiple effects of antioxidant, antibacterial, long-acting moisturizing and physical isolation, and can continuously and stably exert the multiple biological activities thereof.

Antibody or binding fragment thereof, fusion protein, or pharmaceutical composition directed against misfolded SOD1 epitope, or nucleic acid encoding the antibody or binding fragment thereof, fusion protein, or pharmaceutical composition, are described. Also provided are methods for using and manufacturing such antibody or binding fragment thereof, fusion protein, nucleic acid, or pharmaceutical composition, as well as methods of their use for treating a disorder in a human subject in need thereof.

The application discloses a kind of synthetic functional microbial inoculants and its application in salt-tolerant growth promotion of wheat, adopt hydrophobic gordon's bacteria ( Gordonia hydrophobica ), odyssey lysine bacillus ( Lysinibacillus odysseyi ), sea bed motility microorganism ( Planomicrobium okeanokoites ), north China broussonetia yellow color bacterium ( Luteimonas huabeiensis ), epidermis brevibacterium ( Brevibacterium epidermidis ) five strains of bacteria, microbial inoculant has no antagonism between strains, salt-tolerant, and other significant characteristics such as viable count, can significantly promote spring wheat germination rate, seedling length and root length, and has significant promoting effect on seedling length and root length of winter wheat.Under salt stress conditions, the microbial inoculant can significantly improve the activities of catalase, peroxidase and superoxide dismutase of wheat, and the total chlorophyll content;after treatment with the microbial inoculant, the contents of malondialdehyde and proline of wheat are significantly reduced.The microbial inoculant plays a promoting and protecting role in the process of resisting salt stress of spring wheat and winter wheat, and has an impact on the development and sustainable development of arid region agriculture.

This invention discloses a bio-enzyme micronutrient water-soluble fertilizer capable of degrading pesticide and fertilizer residues. The water-soluble fertilizer comprises: basic nutrient components including boron, zinc, and manganese-zinc; bioactive components including superoxide dismutase (SOD) and a complex enzyme system; and a carrier synergist component including fulvic acid and amino acids. The concentrations of boron, zinc, and manganese-zinc in the water-soluble fertilizer are 25 g / L, 50 g / L, and 25 g / L, respectively. Compared with conventional single-element micronutrient fertilizers and ordinary water-soluble fertilizers, this fertilizer, employing amino acid chelation technology and a multi-enzyme synergistic system, effectively solves problems such as poor nutrient absorption, low utilization rate, and element antagonism in traditional fertilizers. It can comprehensively activate crop physiological metabolism, improve photosynthetic efficiency and nutrient transport capacity, and achieve stable and increased yields.

The application provides a preparation method and application of hollow cerium dioxide nanoparticles, and the preparation method comprises the following steps: (1) preparing nanosilica particles by a solvothermal method to obtain a powder; (2) generating a cerium dioxide shell layer on the surface of the nanosilica particle template by the solvothermal method to obtain a powder; (3) calcining the obtained powder; and (4) etching the core-shell structure nanoparticles by alkali to obtain the hollow cerium dioxide nanoparticles. The nanoscale hollow cerium dioxide nanoparticles are synthesized through a simple reaction, the size and morphology are uniform, the synthesis steps are simple, and the hollow cerium dioxide nanoparticles are suitable for batch production. Through experiments, the hollow cerium dioxide nanoparticles have peroxidase and superoxide dismutase enzyme-like activities, so that the hollow cerium dioxide nanoparticles have good scavenging capacity for active oxygen and are suitable for application in biomedical research.

Aspects of this disclosure provide compounds, compositions, and methods for regulating the expression or activity of superoxide dismutase 1 (SOD1). In some aspects, the compounds, compositions, and methods of this disclosure can be used to reduce the expression of SOD1 mRNA in cells or animals. In some aspects, the compounds, compositions, and methods of this disclosure can be used to reduce the expression of SOD1 protein in cells or animals. In certain embodiments, the animal has a CNS-related disease, disorder, or condition.

This invention discloses a marine-derived superoxide dismutase (MhSOD), its preparation method, and its applications. The invention provides a superoxide dismutase MhSOD derived from the hydrothermal bacterium *Marinithermus hydrothermalis*, and establishes an efficient preparation method for MhSOD. Through systematic optimization of expression conditions, the optimal expression conditions were determined: TB medium, a final IPTG concentration of 0.7 mM, and an induction temperature of 30°C. Under these conditions, MhSOD is efficiently expressed in a soluble form. After one-step purification by nickel column affinity chromatography, a single target band free of contaminating proteins is obtained, indicating that this purification process can effectively enrich the target protein.

This invention provides a method and application for in-situ construction of barium titanate piezoelectric nanozymes based on mesoporous silica. Mesoporous silica (MSNs) is used as a carrier, titanium acetylacetonate as the titanium source, barium chloride as the barium source, and ferric chloride hexahydrate as the iron source. After stirring for 24 h and then gradient calcination for 6 h, iron-doped barium titanate piezoelectric nanozymes grown in situ within the pores of MSNs are synthesized. The prepared material exhibits piezoelectric catalytic activity, capable of converting endogenous H2O in tumor cells into hydroxyl radicals (•OH) and O2 into superoxide anions (•O2) under ultrasonic stimulation. ‑ It also possesses peroxidase-like (POD) and superoxide dismutase-like (SOD) activities, and can utilize SOD-like activity to convert •O2 ‑ The process involves converting the generated H2O2 into H2O2, alleviating the H2O2 deficiency within the tumor microenvironment. Then, utilizing POD-like activity, the generated H2O2 and endogenous tumor-derived H2O2 are converted into •OH, achieving a piezoelectric catalysis and enzyme cascade reaction to further increase the •OH level, efficiently killing tumor cells and inducing tumor cell death. This strategy allows for the in-situ development of iron-doped barium titanate piezoelectric nanozymes that simultaneously achieve sonodynamic catalytic therapeutic performance and enzyme catalytic therapeutic performance within the channels of MSNs.

The application discloses a DNA sulfur-modified bacillus subtilis and application thereof in alleviating alcoholic diseases, and relates to the field of biochemistry.The application provides a DNA sulfur-modified bacillus subtilis, which is named as Bacillus subtilis E10-1 and has a preservation number of CCTCC NO: M20253036.It is verified through animal experiments that the DNA sulfur-modified bacillus subtilis E10-1 is more resistant to alcohol than a strain in which sulfur modification is knocked out, can reduce the levels of indexes such as glutathione pyrotransaminase, glutathione transaminase and urea nitrogen in serum of a host after drinking alcohol, and the level of interferon gamma, can increase the levels of total protein, superoxide dismutase and glutathione peroxidase, can alleviate oxidative stress damage caused by alcohol, can reduce inflammatory reactions of various organ tissues, can maintain normal physiological functions of cells, can repair intestinal barriers, and can maintain intestinal flora homeostasis, so that alcoholic related diseases can be prevented and / or treated.

The present application relates to the field of bioengineering, and particularly relates to a mutant of anti-aging protein and a raw material preparation method and application thereof. The present application provides a mutant of superoxide dismutase, wherein the 46th position of the superoxide dismutase is mutated from L to Y; and / or the 52nd position of the superoxide dismutase is mutated from L to V; and / or the 53rd position of the superoxide dismutase is mutated from S to E; and / or the 116th position of the superoxide dismutase is mutated from Q to K; and / or the 157th position of the superoxide dismutase is mutated from S to Q; and / or the 158th position of the superoxide dismutase is mutated from L to D. The present application firstly performs heat treatment (50 DEG C) screening on an SOD enzyme library, and then focuses on a mesophilic bacteria-derived SOD protein, wherein HaSOD is used as a template, and after multiple rounds of iterative mutation, a mutant with high heat resistance, good stability and good enzyme activity is obtained.

This invention discloses a strain of *Bosea vestrisii* and its application in promoting plant growth and improving salt tolerance. This strain is deposited at the China Center for Type Culture Collection (CCTCC) under accession number CCTCC M 2026307. *Bosea vestrisii* exhibits strong salt tolerance and possesses multiple growth-promoting functions, including secreting indoleacetic acid (IAA), synthesizing extracellular polysaccharides, and biological nitrogen fixation. Experiments show that applying this strain to wheat roots significantly increases plant height, root length, and biomass under salt stress, and enhances the activity of antioxidant enzymes (such as catalase, peroxidase, and superoxide dismutase) in the plant, effectively alleviating oxidative damage caused by salt stress. This invention provides a highly efficient and environmentally friendly microbial resource for improving saline-alkali soils, enhancing crop stress tolerance, and achieving sustainable agriculture.

The application discloses application of soybean peptides in fruit preservation. The application discloses application of soybean peptides in fruit preservation, expands the application range of the soybean peptides, and can significantly maintain the hardness, ethylene content, titratable acid content and soluble sugar content of apple fruits, maintain the superoxide dismutase activity of the fruits, reduce browning of the fruits, and enable long-term preservation of the apples and is safe and reliable.

The present disclosure relates to methods of treating a range of diseases or conditions. The methods involve administration of a superoxide dismutase mimetic.

The application discloses a kind of composite enzyme preparation and preparation method thereof, it is related to enzyme preparation technical field.The composite enzyme preparation of the application at least includes the following weight parts of component: xylanase 10-30 parts, modified cellulase 15-35 parts, glucanase 8-20 parts, protease 5-15 parts, amylase 5-18 parts, pectinase 3-12 parts, phytase 5-25 parts, modified superoxide dismutase composite microsphere 15-40 parts.The composite enzyme preparation provided in the application is added in the daily ration of ruminant, can improve the apparent digestibility of ruminant to nutrient and growth performance, while more safe quality better meat quality can be obtained, improve meat taste, obtain higher economic value.

This invention discloses a human-derived *Lactobacillus TG035* for sake brewing and its application in antioxidant and acetaldehyde dehydrogenase-producing products, belonging to the field of microbial technology. The strain TG035 has the preservation number CCTCC NO: M 20252662, and its 16S rDNA sequence is shown in SEQ ID NO.1. This strain possesses the dual function of producing acetaldehyde dehydrogenase and superoxide dismutase.

This invention discloses a cryopreservation diluent containing fucoxanthin and its applications. Adding fucoxant to the cryopreservation diluent enhances the antioxidant capacity of frozen-thawed semen, reduces oxidative damage to goat sperm from reactive oxygen species during freezing-thawing, significantly improves sperm viability, motility, plasma membrane integrity, and total antioxidant capacity after freezing-thawing, enhances catalase and total superoxide dismutase activity, and reduces reactive oxygen species and malondialdehyde content. The effect is best when the fucoxant concentration is 2 μM during cryopreservation. The cryopreservation diluent of this invention is simple to prepare and operate, significantly improves the cryopreservation effect of semen, and has important practical application value.

This invention relates to the application of cobalt phosphide in the preparation of anti-inflammatory drugs, specifically in the field of pharmaceutical formulation. The cobalt phosphide possesses enzyme-like catalytic activities similar to peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD), as well as the ability to scavenge hydroxyl radicals (·OH), making it a broad-spectrum ROS scavenger capable of alleviating inflammation-related diseases. Furthermore, due to its excellent photothermal properties, it can achieve antibacterial effects at inflamed wound sites, and its enhanced catalytic activity through thermal effects and the promotion of tissue wound healing through oxygen generation provide a new direction for developing novel nanomedicines with high ROS scavenging capabilities for the treatment of inflammation, particularly psoriasis.

This invention discloses a composite hydrogel with pH-responsive dual-enzyme activity switching properties, its preparation method, and its applications, belonging to the field of wound healing materials technology. The composite hydrogel comprises a nanozyme and a hydrogel. The nanozyme is a gold-platinum nanozyme, which is porous and dendritic, exhibiting different enzyme-like activities under different pH conditions. In an acidic environment, it exhibits oxidase-like and peroxidase-like activities, while in a neutral environment, it exhibits superoxide dismutase-like and catalase-like activities. This invention can exhibit reversibly switchable multi-enzyme mimicry activities under different pH conditions, thereby achieving dynamic synergistic regulation of antibacterial and antioxidant effects, overcoming the problem of difficulty in simultaneously achieving both in existing technologies. Furthermore, this invention has good biocompatibility, adhesion, and sustained-release properties, enabling adaptive functional regulation at different stages of wound healing, significantly promoting chronic wound healing and tissue repair, and showing promising clinical application prospects.