141 results about "Dismutase" patented technology

This invention relates to the field of microbial technology, specifically to a strain of *Lactobacillus rhamnosus* and its uses. The preservation number of the *Lactobacillus rhamnosus* strain is CGMCC No. 25682. The *Lactobacillus rhamnosus* strain of this invention exhibits excellent free radical scavenging, oxidative stress response, antioxidant, and anti-aging capabilities. It can increase the levels of superoxide dismutase, glutathione peroxidase, and glutathione, decrease malondialdehyde levels, and improve the scavenging rate of hydroxyl radicals and DPPH radicals. It can produce DPP-4 and α-glucosidase inhibitors and stimulate NCI-H716 cells to produce GLP-1, demonstrating potential for blood glucose regulation. The *Lactobacillus rhamnosus* strain of this invention can also be used to ferment and produce fermented dairy products, fermented oat products, and other fermentation products.

The application discloses a superoxide dismutase biological anti-stress nutrient fertilizer based on plant extraction and a production method thereof. The fertilizer contains, in percentage by mass, 1.0-10.0% of superoxide dismutase, 1.0-5.0% of a plant anti-stress inducing factor complex, 0.1-2.0% of organic chelated trace elements, 5.0-15.0% of a phosphorus-potassium enhancing component, 0.5-5.0% of a natural protective agent, 3.0-10.0% of a penetrating agent, 0.5-15.0% of a microbial metabolic synergist, 0.1-5.0% of a pH buffer component, 0.01-0.5% of a plant-derived preservative, and the balance of water. The superoxide dismutase is preferably derived from corn germ or spinach extract. The production method comprises the steps of enzyme activity component preparation, water phase base preparation, anti-stress function phase preparation, step-by-step mixing and primary stabilization, final product integration and secondary stabilization, and the like. Through scientific compounding and synergy of various biological active components, the application significantly enhances the resistance of crops to drought, saline-alkali, high and low temperature and the like, and promotes growth and quality improvement.

This disclosure relates to compositions and methods using genetically modified bacteria that express superoxide dismutase (SOD) and catalase enzymes to reduce oxidative stress in the skin, and can be used for various skin conditions. In some embodiments of this disclosure, the genetically modified bacteria are Gram-positive bacteria, for example, of the genus Corynebacterium.

The invention relates to the technical field of nano-materials, and discloses a heterogeneous diatomic nano-enzyme as well as a preparation method and application thereof. The invention provides a space confinement regulation and control strategy which is used for constructing a heterodiatomic catalytic center on a nitrogen-doped carbon skeleton and preparing nano-enzyme with structural stability and synergistic catalytic performance. According to the method, a confinement framework formed by self-assembly of first metal ions such as cobalt ions and second metal ions such as zinc ions and organic ligands is used as a template, third metal ions such as iron ions are introduced, and then carbonization heat treatment is performed in a protective gas atmosphere, so that bimetal sites with stable bridging configuration are formed in situ. The obtained nano-enzyme has the catalytic activity of various antioxidant enzymes, including catalase and remarkable superoxide dismutase activity, and has more excellent free radical scavenging capacity compared with a single-metal or non-bridging system.

The application provides application of a calcium sensing protein ShCBL1 gene and belongs to the technical field of biology.The application overexpresses or superexpresses the ShCBL1 gene in sugarcane or rice, so that the content of proline and superoxide dismutase in the sugarcane or rice is increased, the content of malondialdehyde is reduced, and the cold resistance is improved.Researches show that when the ShCBL1 is superexpressed or overexpressed in plants, the cold stress related physiological indexes such as proline, superoxide dismutase (SOD) and malondialdehyde (MDA) can be significantly affected, so that the cold resistance of the rice and the sugarcane is enhanced.ShCBL1 as a positive regulator of cold resistance regulation can be used for molecular breeding of cold resistance varieties.

Provided is a test method with which mild cognitive impairment (MCI) is detectable and a measurement reagent for use in the test method. A test method for MCI according to the present invention includes a step of measuring the activity of superoxide dismutase (SOD) in a biological sample of a subject.

The present invention refers to a composition comprising Cutibacterium acnes (C. acnes) bacterial strains, wherein said strains are characterized by being modified to secrete a heterologous protein by insertion of an expression construct comprising at least one or more transcription promoter sequences, one or more ORFs (Open Reading Frames) encoding the heterologous protein, and one or more transcription termination sequences; wherein the heterologous protein is characterized by comprising at the N-terminus of the protein sequence, a secretion signal which is processed by C. acnes to secrete the protein, and wherein the heterologous protein is the superoxide dismutase enzyme.

The invention relates to the field of cosmetic compositions, in particular to a cosmetic composition containing triple polishing enzyme and a preparation method of the cosmetic composition. The triple polishing enzyme comprises protease, protease K and superoxide dismutase, the weight part ratio of the protease to the protease K to the superoxide dismutase is 1: (0.5-2): (0.5-2), the total mass percent of the triple polishing enzyme in the composition is 0.0002%-0.001%, and the invention further discloses a preparation method of the cosmetic composition. A triple polishing enzyme synergistic system is formed through scientific compounding, the integrated skin care effect under low concentration is achieved, and the defects of a traditional chemical stripping agent and the shortages of a single enzyme preparation are overcome; meanwhile, by optimizing the proportion of formula components, the composition forms a liquid crystal structure, so that long-acting dual moisturizing of internal water locking and external water sealing is realized, triple polishing enzyme can be wrapped and protected, and the problems that the composition is easy to oxidize and inactivate and low in transdermal efficiency are solved.

The invention discloses application of pseudopenicillin variotii in preparation of a resistance inducer for preventing and treating plant diseases, and belongs to the technical field of plant protection. The invention provides an application of Panax variotii in prevention and treatment of plant diseases, the application concentration of the Panax variotii is 0.1 microgram / mL-1 microgram / mL, a plant resistance inducer containing the Panax variotii can be sprayed on plant leaves or fruit surfaces and has a remarkable prevention effect on anthrax leaf blight, and the higher the concentration is, the better the prevention effect is; the pseudopenicillin variotii can promote synthesis or activity improvement of catalase, peroxidase, polyphenol oxidase, glutathione S-transferase and superoxide dismutase in apple bodies, and also can remarkably induce up-regulation expression of disease-resistant related genes such as MdPR2 and MdPR5, so that the disease resistance, the antioxidant level and the plant immunity of apples are enhanced; a novel and efficient plant resistance inducer application scheme is provided for apple disease control and physiological regulation.

This invention relates to the field of bioengineering technology and provides an application of the maize heat shock transcription factor gene ZmHSF18, wherein the nucleotide sequence of the maize heat shock transcription factor gene ZmHSF18 is shown in SEQ ID NO:1. Experiments have demonstrated that overexpression of the maize heat shock transcription factor gene ZmHSF18 in plants can significantly improve seed germination rate and frozen survival rate at the mature stage under low-temperature stress, reduce malondialdehyde accumulation, and increase superoxide dismutase activity. Therefore, this maize heat shock transcription factor gene ZmHSF18 can be used to improve plant cold tolerance and create transgenic plants with enhanced cold tolerance, possessing important application value in plant genetic engineering and stress resistance breeding.

The invention provides superoxide dismutase, a preparation method thereof and application of the superoxide dismutase in screening and killing active substances of ampullaria gigas, and belongs to the technical field of biology, and the sequence of the superoxide dismutase is shown as SEQ ID No.1. A compound capable of killing the ampullaria gigas can be rapidly screened out aiming at an SOD target spot of the ampullaria gigas, and the compound can be used for killing the ampullaria gigas. Compared with traditional bred ampullaria gigas screening, the screening method is high in speed and low in cost.

This invention discloses the application of the blueberry VcERF70 gene in improving cadmium tolerance in plants, belonging to the field of plant genetic engineering technology. The amino acid sequence of the VcERF70 protein is shown in SEQ ID NO.2, and the nucleotide sequence of its encoding gene is shown in SEQ ID NO.1. This invention constructs a plant overexpression vector containing the VcERF70 gene and genetically transforms it into blueberries. The resulting transgenic plants exhibit significantly reduced malondialdehyde and hydrogen peroxide content under cadmium stress, and significantly increased superoxide dismutase, catalase, peroxidase activities, and glutathione content. Experiments demonstrate that overexpression of the VcERF70 gene can effectively enhance the antioxidant defense capacity of blueberries, reduce cadmium stress-induced oxidative damage, and thus improve the blueberry's tolerance to cadmium. This invention provides a new gene resource and technical pathway for blueberry stress-resistant breeding.

The application relates to a composition for treating reflux esophagitis and application thereof. The composition comprises the following components in parts by weight: 2-8 parts of scutellaria baicalensis, 3-7 parts of licorice, 5-10 parts of beeswax and 80-120 parts of vegetable oil. The composition for treating reflux esophagitis contains scutellaria baicalensis, licorice, beeswax and vegetable oil, and through mutual cooperation among the components, the drug prepared from the composition can significantly increase the plasma motilin level of a patient, further increase the pressure of the lower esophageal sphincter, promote gastric emptying and prevent acid reflux, promote the recovery of reflux esophagitis, the esophagus of the patient is obviously improved, and no adverse reaction is found; meanwhile, the content of lipid peroxide can be obviously reduced, the activities of superoxide dismutase and glutathione peroxidase are increased, the esophageal mucosa is protected and treated, the pathological changes of the esophagus are obviously improved, and the treatment effect is excellent.

The invention belongs to the technical field of protein engineering, particularly relates to a protein tag with superoxide dismutase activity and application, and provides a protein tag which comprises polypeptide or protein with superoxide dismutase activity. On the second aspect, a fusion protein is provided and comprises the protein tag described in the first aspect and at least one target polypeptide or protein, the target polypeptide or protein is not superoxide dismutase, and the target polypeptide or protein is not superoxide dismutase only by using the protein tag disclosed by the invention. According to the present invention, the technical effects of target polypeptide or protein expression quantity detection (SOD enzyme activity detection), target polypeptide or protein protection from oxidative damage, target polypeptide or protein stability improvement and target polypeptide or protein solubility improvement can be simultaneously achieved without adding any additional step compared with the fusion protein expression operation in the prior art; the operation is convenient.

This invention belongs to the field of ecological environment monitoring technology and relates to a multi-level bioindicator method for the effective phytotoxicity of heavy metal-like substances / heavy metals. The method includes the following steps: S1. Obtaining plant samples affected by the soil or water body to be tested; S2. Measuring the activity of superoxide dismutase (SOD), gibberellin (GA), and chlorophyll content in the plant samples; S3. Based on the measured results of SOD activity, plant hormone content, and chlorophyll content, and their changes over time, classifying and assessing the effective phytotoxicity effects of heavy metal-like substances or heavy metals. This invention is the first to propose a unified three-level bioindicator method (SOD activity, GA hormone, and chlorophyll) that can effectively reflect soil pollution levels for typical heavy metal-like substances / heavy metals present in the actual environment, enabling a simpler assessment of the degree of heavy metal pollution in actual soil.

This application belongs to the field of microbial technology, and mainly relates to a strain of *Pediococcus lactis* BPA03 and its applications. *Pediococcus lactis* BPA03 is classified and named *Pediococcus lactis*. Pediococcus acidilactici This *Pediococcus lactis* strain BPA03 was deposited on September 28, 2025, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 36109. It inhibits skin melanin deposition and improves UV photoaging, suppresses the gene expression levels of immune factors such as IL-1β, IL-6, IL-8, and TNF-α, improves the activities of superoxide dismutase, catalase, and glutathione peroxidase, inhibits vinorelbine-induced neutropenia, and improves the inhibition of immune factor expression induced by vinorelbine, thus exhibiting comprehensive anti-aging effects.

The present disclosure relates to methods of treating a range of diseases or conditions. The methods involve administration of a superoxide dismutase mimetic.

This invention provides a composition for alleviating intestinal oxidative stress damage and its application, the composition comprising 4'-galactosyllactose (4'-GL) and glycomacropeptide (GMP). This invention found that, compared with the individual concentrations of 4'-GL and GMP, the composition of 4'-GL and GMP more significantly promotes the release of superoxide dismutase and catalase, while also more significantly reducing the release of malondialdehyde, a marker of oxidative stress. The synergistic effect between the two allows 4'-GL and GMP to better alleviate intestinal damage in Drosophila. Simultaneously, the composition can improve the survival rate and climbing index of H2O2-induced Drosophila, prolong the intestinal length of diseased Drosophila, improve the integrity of the intestinal barrier, and induce a recovery trend in the Drosophila. This demonstrates that the composition has a mitigating effect on intestinal oxidative stress damage.

This invention relates to the field of plant disease biological control technology, and particularly to a Fusarium oxysporum cell wall extract, its preparation method, and its application. The extract is derived from the polysaccharide fraction of the cell wall of Fusarium oxysporum spores or hyphae, and contains at least one pathogen-associated molecular pattern (PAMP) component. This component is a non-protein, thermally stable, species-nonspecific elicitor capable of forming an aqueous dispersion system. The extract of this invention can be recognized by plant PRRs and activate PTI immunity. Rehmannia glutinosa tubers treated with the extract showed a significantly increased germination rate after inoculation with Fusarium oxysporum. It also induces superoxide anion production, upregulates the activities of superoxide dismutase, peroxidase, and catalase, and enriches differentially expressed genes in the MAPK signaling pathway, jasmonic acid metabolism pathway, and phenylpropane biosynthesis pathway. Furthermore, the extract can directly inhibit Fusarium oxysporum colony expansion, conidia production, and mycelial growth. Extracts derived from spores or hyphae show no significant difference in inducing disease resistance, and the raw materials are flexible and easy to industrialize. This extract is derived from the cell wall of the pathogen itself, making it environmentally friendly and free from the risk of chemical pesticide residues. It is suitable for the green control of root rot disease in Rehmannia glutinosa.

The application discloses an application of near-infrared controllable Fe, Zn doped tea polyphenol based carbon dot nanometer enzyme in fruit preservation, and belongs to the technical field of agricultural product preservation.The application takes tea polyphenol as a precursor, and prepares Fe, Zn doped carbon dots (Fe, Zn@GTP) nanometer enzyme with excellent antioxidant and antibacterial activity through a microwave assisted method.The Fe, Zn@GTP nanometer enzyme exhibits near-infrared light enhanced peroxidase, superoxide dismutase and catalase catalytic activity and photo-thermal performance.The Fe, Zn@GTP has antibacterial effects on escherichia coli, staphylococcus aureus, methicillin-resistant staphylococcus aureus, ampicillin-resistant escherichia coli and penicillium expansum, and the inhibition rate of the bacterial biofilm treated by the Fe, Zn@GTP is more than 90%.In the preservation experiment, the PVA / Fe, Zn@GTP film can effectively prolong the preservation period of fruits, significantly reduce the occurrence rate of fruit mold and browning, and the deterioration degree of quality indexes.

This invention relates to a microbial composition for preventing and controlling root rot in tea trees and its application, belonging to the field of microbial and organic fertilizer technology. The composition comprises the following components: Component 1, Bacillus kaempferi and Trichoderma yunnanensis; Component 2, humic acid, Chrysanthemum indicum extract, amino acids, brown algae oligosaccharides, complex vitamins, and trace elements; wherein, Bacillus kaempferi is N4471 (…). Bacillus cabrialesii The accession number is CCTCC NO: M20221660, and the Yunnan Trichoderma is YNK-FG0001 ( Trichoderma yunnanense The microbial composition, with accession number CCTCC NO: M 2025645, significantly improved soil water stability aggregates, pH, and cation exchange capacity when applied to tea plant cultivation. It also enhanced root vitality and the activity of antioxidant enzymes [superoxide dismutase (SOD) and peroxidase (POD)], thereby reducing the growth of Fusarium rot (…). Fusarium solani ), Pythium ( Pythium spinosum ) and teosinte ( Rosellinia arched The disease index of tea tree root rot caused by infection was used to ultimately achieve effective control of tea tree root rot.

The invention discloses a preparation method of high-stability superoxide dismutase (SOD), and belongs to the technical field of bioengineering and enzyme engineering. The method comprises the following steps: chemically modifying purified SOD (superoxide dismutase) under the conditions of low temperature and neutral pH (potential of hydrogen) by taking monomethoxy polyethylene glycol succinimido active ester as a modifier; carrying out ultrafiltration preliminary purification on the reaction liquid, then carrying out deep purification through ion exchange chromatography, and separating to obtain a high-purity modified product; and finally, sterilizing, filtering and freeze-drying to obtain the freeze-dried powder. According to the method, by optimizing a modification and purification process, the thermal stability and protease degradation resistance of SOD are remarkably improved, and meanwhile, most enzyme activity of SOD is effectively reserved. The prepared PEG-SOD product is high in purity and good in stability, and is suitable for preparing anti-inflammatory, anti-oxidation or anti-aging medicines and functional foods.

The present application provides a composition with satiety and weight control and anti-aging, and a preparation method and application thereof. The composition comprises the following raw materials by weight: polysaccharide 11-13 parts, fiber powder 20-25 parts, highland barley powder 12-18 parts, compound powder 18-23 parts, pectin 4-8 parts, ganoderma lucidum powder 6-9 parts, and compound protein 8-12 parts, and relates to the technical field of satiety and weight control and anti-aging compositions. The composition can form an effect of hindering nutrient absorption or prolonging gastric emptying, increase the volume of intestinal contents to achieve satiety, thereby achieving the functions of well controlling weight gain and reducing body weight. In addition, the composition can activate the body's antioxidant enzyme system, improve the activity of superoxide dismutase (SOD), and reduce the content of malondialdehyde (MDA), thereby delaying cell aging and achieving the anti-aging effect.

The invention belongs to the field of biology, and discloses a tissue formula preserving fluid containing antimycin A. The tissue formula preserving fluid comprises a basic culture medium, the basal culture medium contains superoxide dismutase, catalase and antimycin A. On the basis of the superoxide dismutase and the catalase, the preservation solution is matched with the antimycin A, so that the activity of tissue cells can be remarkably improved; in the research process, we have the following specificity that: 1, when the respiratory chain inhibitor drug is applied to the formula of the invention, the improvement degree of the cell viability of only antimycin A is the highest; 2, the function of antimycin A must be exerted based on the use of superoxide dismutase and catalase; meanwhile, the invention further provides a tissue preservation method.

The present invention relates to superoxide dismutase and uses thereof for preventing or treating diabetic retinopathy or uveitis. The superoxide dismutase according to the present invention can be effectively used in the prevention or treatment of diabetic retinopathy or uveitis, particularly through oral administration.

The invention belongs to the field of medicines, and relates to a scutellarin aglycone derivative as well as a preparation method and application thereof. The scutellarin aglycone derivative has a structure as shown in a formula I. The preparation method comprises the following steps: reacting scutellarin (a compound II) with methanol to obtain a compound III, then reacting with bornyl chloroacetate (a compound IV) to obtain a compound V, and finally carrying out an alkaline hydrolysis reaction to obtain the compound I, the scutellarin aglycone derivative has the effect of preventing or treating cardiovascular and cerebrovascular related diseases, can improve the activity of superoxide dismutase (SOD), inhibit the production of lactic dehydrogenase (LDH) and reduce the malondialdehyde (MDA) level superior to that of scutellarin, has the inhibiting effect on inflammatory factors TNF-alpha, IL-6 and IL-1beta in cell supernatant superior to that of scutellarin, and can be used for preparing a medicine for treating cardiovascular and cerebrovascular related diseases, such as cardiovascular and cerebrovascular related diseases, cardiovascular and cerebrovascular related diseases, cardiovascular and cerebrovascular related diseases, cardiovascular and cerebrovascular related diseases, cardiovascular and cerebrovascular related diseases, cardiovascular and cerebrovascular related diseases and cardiovascular and cerebrovascular related diseases. The invention has the potential of preparing or preventing cardiovascular and cerebrovascular related diseases.

The invention relates to a rhodium nano-enzyme compound and application thereof in preparation of drugs for preventing, relieving and / or treating drug-induced liver injury. The rhodium nano-enzyme compound comprises an extracellular vesicle simulant and rhodium nano-enzyme located in the extracellular vesicle simulant. The surface of the rhodium nano-enzyme is coated with the extracellular vesicle simulant, the extracellular vesicle simulant has good biocompatibility and low immunogenicity and can promote tissue repair and regeneration, and the extracellular vesicle simulant can be removed in a non-specific mode and then is enriched in the liver in a targeted mode, so that the rhodium nano-enzyme is enriched in the liver together with the extracellular vesicle simulant. The rhodium nano-enzyme has excellent simulation activity of superoxide dismutase (SOD) and catalase (CAT), can efficiently remove various active oxygen species, and can inhibit inflammatory reaction caused by APAP, so that the rhodium nano-enzyme has a targeted treatment effect on drug-induced liver injury.

Disclosed is an antioxidant formulation having enhanced absorption and antioxidant activity to address the challenges encountered in utilizing antioxidant oral food supplements. Said antioxidant formulation comprising S-acetylglutathione (SAG), superoxide dismutase (SOD), and maltodextrin. Preferably, an antioxidant formulation as an oral food supplement comprising 100 - 1000 mg SAG, 50 - 300 IU SOD, and 10 - 100 mg maltodextrin.

The application provides a temperature-sensitive antibacterial and antioxidant gel, and a recombinant fusion protein sequence with antibacterial and antioxidant activity used in the gel is recombined by 1 human-derived superoxide dismutase (hSOD1), 1 rigid dipeptide and 1 endolysin (TSPpgh) sequence, wherein the human-derived superoxide dismutase (hSOD1) sequence is connected with the endolysin (TSPpgh) sequence through the rigid dipeptide (LP) to facilitate the respective protein activity, then the recombined fusion protein is used as antibacterial and antioxidant raw material, mixed with a temperature-sensitive chitosan hydrogel base, and finally prepared into the temperature-sensitive antibacterial and antioxidant hydrogel. The composite temperature-sensitive hydrogel has multiple effects of antioxidant, antibacterial, long-acting moisturizing and physical isolation, and can continuously and stably exert the multiple biological activities thereof.

The invention provides rhodopseudomonas palustris PSB-S, a biocontrol microbial agent thereof and application of the rhodopseudomonas palustris PSB-S in prevention and treatment of kiwifruit canker, and belongs to the technical field of biopesticides. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on November 17, 2025, and the preservation number is CGMCC No.36662. The invention further discloses a preparation method of the strain. According to the method, the biocontrol inoculant is obtained by culturing the rhodopseudomonas palustris PSB-S, and the kiwifruit canker is prevented and controlled by spraying the whole plant. The application of the biocontrol inoculant can induce the increase of the activity of defensive enzymes of kiwi fruit plants, and the defensive enzymes comprise at least one of peroxidase (POD), superoxide dismutase (SOD), phenylalanine ammonialyase (PAL) and catalase (CAT). By applying the biocontrol inoculant, the expression of immune response genes NPR1 and FLS2 of kiwi fruit plants can be up-regulated, and the biocontrol inoculant is environment-friendly and has no acute toxic harm. The invention is expected to provide a direction for prevention and control of kiwifruit canker and sustainable development of agriculture in China.