357 results about "Dismutase" patented technology

The invention provides a nano transdermal delivery system based on superoxide dismutase, the nano transdermal delivery system is of a capsule composite structure of a small molecule active component-superoxide dismutase-polymer shell layer, the small molecule active component is loaded in superoxide dismutase, the surface of the superoxide dismutase is coated by the polymer shell layer, and the superoxide dismutase is loaded in the capsule composite structure. The diameter of the superoxide dismutase-based active ingredient transdermal delivery system is 20-100 nm, and the thickness of the polymer shell layer is 7.5-52.5 nm. In the delivery system, the polymer shell layer provides a protection effect for the superoxide dismutase and the micromolecular functional substances with the anti-oxidation effect, the capsule composite structure is in a nanogel form, and the nanogel not only promotes the penetration depth of the capsule, but also reduces the irritation effect of the capsule on the skin. The raw materials are biologically friendly, the preparation method is simple, the yield is high, and industrial large-scale production can be realized.

The invention provides a preparation method of medicinal liquor and application of the medicinal liquor in preparation of antioxidant and anti-aging products, and relates to the technical field of medicinal preparations. The preparation method comprises the following steps: (1) crushing; (2) extracting; (3) standing and separating; and (4) freezing and filtering to obtain the medicinal liquor. The medicinal liquor is obtained by extracting the traditional Chinese medicine components through the steps of crushing in batches, mixing and extracting, standing, freezing and separating the supernate and the suspension in different separation modes, and the medicinal liquor can better improve the space exploration capability of a mouse aged due to D-galactose, can reduce malondialdehyde MDA, and can improve the immunity of the mouse. The activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) is improved, and the obvious anti-oxidation and anti-aging effects are achieved.

The invention discloses a tumor cholesterol metabolism regulation microneedle patch and a preparation method, the microneedle patch comprises a needle tip and a backing which are connected, the needle tip comprises a needle tip body and nanoparticles loaded on the needle tip body, the nanoparticle is a manganese ion-doped organic metal framework, the outer layer of the manganese ion-doped organic metal framework is modified with a targeting agent, cholesterol oxidase and superoxide dismutase are carried on the manganese ion-doped organic metal framework, the targeting agent can target a CD44 receptor, and the organic metal framework can carry drugs. The targeted drug can enter tumor cells in a targeted mode, superoxide dismutase catalyzes superoxide anions overexpressed in the tumor cells to generate H2O2 and O2, O2 needed by catalysis is provided for cholesterol oxidase, cholesterol at the tumor site is consumed by the cholesterol oxidase, accumulation of 7-DHC is reduced, meanwhile H2O2 can be generated, and the cholesterol oxidase can be used for catalyzing the tumor site. H2O2 is catalyzed by manganese ions through a Fenton-like reaction to generate OH to induce tumor cells to generate ferroptosis, so that ferroptosis-immune synergistic treatment is realized.

The invention discloses bacillus subtilis ZX-5 for promoting growth of gleditsia sinensis under salt stress and application of the bacillus subtilis ZX-5, and relates to the technical field of microorganisms. The invention discloses bacillus subtilis ZX-5 for promoting growth of gleditsia sinensis under salt stress, and the bacillus subtilis ZX-5 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.31648. The result of the embodiment shows that the bacillus subtilis ZX-5 promotes the ground diameter growth rate and seedling height growth rate of the gleditsia sinensis to be improved under salt stress, and promotes the total root length and root tip number of the gleditsia sinensis root system to be increased; the surface area of the gleditsia sinensis root is increased under the stress of 150mM salt. The activities of Chinese honey locust superoxide dismutase, peroxidase, catalase and ascorbic acid peroxidase are promoted to be enhanced under the stress of salt; the contents of ascorbic acid and reduced glutathione in the gleditsia sinensis are promoted to be increased under the stress of salt; the content increase of soluble protein, soluble sugar and free proline in the gleditsia sinensis is promoted.

The invention provides an antioxidant enzyme-containing composition and a preparation method thereof, and relates to the technical field of enteritis treatment. The preparation method comprises the following steps: S1, collecting various component materials for preparing the antioxidant enzyme-containing composition: collecting various raw materials for preparing glucan, fructo-oligosaccharide, galactooligosaccharide, superoxide dismutase, vitamin C, bunge cherry seed powder, cassia seed powder, turmeric, sorbitol, hydroxypropyl methyl cellulose, silicon dioxide and magnesium stearate; according to the invention, a matched powder bagging machine is adopted to realize full-automatic unattended operation to automatically complete the large bag filling and sealing step in the steps S4-S10, vacuumizing is performed firstly and then sealing is performed, and the storage space is compressed by vacuumizing, so that the condition that the bag is expanded and broken by internal air pressure in the transfer and storage process is not easy to cause; in the bag sealing process, the phenomena of high hot melting efficiency and high sealing speed are achieved by adopting a bag strip guiding and rolling hot melting mode.

The invention provides a coordination nano assembly of chiral cyclopeptide and manganese ions as well as a preparation method and application of the coordination nano assembly. According to the method, a manganese superoxide dismutase protein structural domain and a unique tumor microenvironment of melanoma high tyrosinase are combined, and polypeptide sequences L-YD-hL-DD-h, L-YL-HL-DL-H and D-yD-hD-dD-h with different chirality are designed for the first time; the chirality of the polypeptide is regulated and controlled, the in-vivo circulation stability and the cell entry efficiency of the nano assembly are improved, the coordination self-assembly of manganese and different chiral cyclopeptides is realized, and the in-situ oxidation of an in-vivo tumor microenvironment is simulated for photo-thermal therapy and cell membrane coating. The method disclosed by the invention is simple, mild in experimental condition and easy to operate, the prepared cell membrane coated nano assembly not only has long circulation stability and high cell entry efficiency, but also can realize mild photo-thermal therapy through in-situ oxidation, releases manganese ions to activate a CGAS-STING pathway for immunotherapy of tumors, and has a wide application prospect. The potential application value is realized in the field of combined treatment of tumors.

The invention belongs to the technical field of catalytic materials, and particularly relates to high-entropy alloy nanoparticles used as a biocatalyst and application of the high-entropy alloy nanoparticles. The PtFeCuCoNi high-entropy alloy nano-particles with pH adaptability are developed through a one-step solvothermal synthesis method, it is found that the PtFeCuCoNi high-entropy alloy nano-particles can serve as potent oxidase, can generate reactive oxygen species (ROS) and effectively kill bacteria such as MRSA under the acidic condition, and can play the functions of superoxide dismutase and catalase under the neutral condition, so that the PtFeCuCoNi high-entropy alloy nano-particles with the pH adaptability can be used for effectively killing bacteria such as MRSA. Active oxygen in cells is efficiently removed, oxidative stress is relieved, and the cell protection effect is achieved.

The invention discloses a method for evaluating and screening salt tolerance of triticale germplasm resources in a germination stage, and belongs to the technical field of evaluation and screening of plant salt tolerance, salt stress concentration screening: triticale seeds are treated by NaCl solutions with different concentrations, indexes such as survival rate, plant height and overground part fresh weight are measured, the NaCl solution with the concentration of 200 mmol. L <-1 > is determined as the salt tolerance evaluation appropriate concentration, and the salt stress concentration is determined as the salt tolerance evaluation appropriate concentration. Salt tolerance evaluation test: carrying out salt stress treatment by adopting a 200mmol. L <-1 > NaCl solution, determining phenotypic indexes such as survival rate, plant height, fresh weight of overground part, root length and chlorophyll content, and physiological and biochemical indexes such as catalase, peroxidase and superoxide dismutase activity and malondialdehyde content, and carrying out principal component analysis, membership function analysis and clustering analysis to obtain a salt tolerance evaluation result. Calculating a salt tolerance comprehensive evaluation value, and screening salt tolerance triticale germplasm resources.

The invention discloses marasmius albus DCY6478 as well as a culture medium and application thereof, and belongs to the technical field of microorganisms. The invention discloses a Marasmilla sp. DCY6478 strain, and the preservation number of the Marasmilla sp. DCY6478 strain is GDMCC (China General Microbiological Culture Collection Center) No.65883. The Marasmilla sp. Experimental verification results show that the culture medium formula most suitable for growth of the white marasmius is prepared from the following components in percentage by weight: 40% of apple sawdust, 36% of corncob, 14% of cottonseed hull, 1% of lime and 1% of gypsum; the fermentation liquor is verified to have an inhibition effect on a plurality of pathogenic bacteria; the DPPH free radical scavenging capacity of the fermented hyphae is verified, the superoxide dismutase activity and catalase activity are both higher than those of sporocarp, and the fermented hyphae can be used as a natural antioxidant source; it is verified that fermented hyphae or sporocarp of the strain have the capacity of secreting amino acid and polysaccharide and producing crude protein, and a new raw material selection is provided for the industries of food, medicine and the like.

Provided is a superoxide dismutase-based nanoscale transdermal delivery system, which is a capsule composite structure consisting of a small molecule active ingredient, superoxide dismutase, and a polymer coating. The small molecule active ingredient is loaded in the superoxide dismutase, and the surface of the superoxide dismutase is coated with the polymer coating. The superoxide dismutase-based active ingredient transdermal delivery system has a diameter of 20-100 nm, and the polymer coating has a thickness of 7.5-52.5 nm. In the delivery system, the polymer coating protects the superoxide dismutase and the small molecule functional substance having an antioxidant effect, and the capsule composite structure is in the form of nanogel. The nanogel not only promotes the penetration depth of the delivery system, but also mitigates the stimulation of the delivery system to the skin. The delivery system features biofriendly starting materials, simple preparation method, and high yield, and thus can be produced in large scale industrially.

The invention belongs to the technical field of enzyme engineering, and particularly relates to a codon optimized superoxide dismutase AfSOD gene and application thereof in degradation of aflatoxin B1 and zearalenone. Under the conditions that the pH is 7, the temperature is 40 DEG C and the enzyme addition amount is 10 micrograms, the degradation rate of the AFB1 reaches 99.08% within 24 hours, and the degradation rate is kept to be 80% or above within 40-80 DEG C; under the conditions that the pH is 8, the temperature is 60 DEG C and the enzyme addition amount is 20 micrograms, the degradation rate of ZEN reaches 85.1% in 24 hours, and the degradation rate is kept to be 80% or above within 50-80 DEG C; the result shows that AfSOD is a toxin degrading enzyme which can tolerate a certain degree of high temperature; the degradation rates of AFB1 and ZEN in polluted peanut powder or corn powder are 73.7% and 88.2% respectively; the invention provides a new candidate for detoxification of AFB1 and ZEN in food or feed, and has a good application prospect.

The invention relates to a modified superoxide dismutase pH response type nano-liposome and a synergistic anti-photoaging after-sun repair composition capable of achieving accurate targeting. The modified superoxide dismutase pH response type nano-liposome is obtained by modifying a superoxide dismutase nano-liposome with hyaluronic acid, and the modified superoxide dismutase pH response type nano-liposome has the advantages that the modified superoxide dismutase pH response type nano-liposome can be accurately targeted by the aid of the synergistic anti-photoaging after-sun repair composition; the superoxide dismutase nano-liposome is of a core-shell structure, a core layer is superoxide dismutase, a shell layer is a ternary composite liposome, and the ternary composite liposome is formed by compounding HSPC, DSPC and cholesterol. By constructing a pH response type nano delivery system, the pH drop characteristic of an ultraviolet damage area can be accurately recognized, controlled release of SOD at a target site is triggered, O2 <-> and H2O2 are synchronously removed, an oxidative stress chain reaction is blocked, and in cooperation with the inhibition of tetrahydrocurcumin on a tyrosinase signal channel and the anti-inflammatory repair effect of the aloe extract, the anti-inflammatory repair effect of the aloe extract on the tyrosinase signal channel is achieved. A three-dimensional action network of targeted removal-activity regeneration-barrier reconstruction is formed, and the photoaging prevention and treatment effect is improved.

The present invention relates to intracellular copper zinc superoxide dismutase inhibitors for protecting a subject from damage caused by radiation outbreak. The invention also relates to kits comprising SOD1 inhibitors and radiosensitizers for cancer cells, to the in vitro use of SOD1 inhibitors for protecting non-cancer cells from ionizing radiation, and to methods, compositions and uses related thereto.

The present application discloses a filamentous fungal mutant strain with high-efficient expression of superoxide dismutase and its application. The filamentous fungal mutant strain of the present application is formed by gene knockout of phytochrome genes PaPhy1 and / or PaPhy2 in the wild-type filamentous fungus Podospora anserina. The filamentous fungal mutant strain of the present application is a mutant strain with gene knockout of phytochrome gene PaPhy1, a mutant strain with gene knockout of phytochrome gene PaPhy2, or a mutant strain with gene knockout of phytochrome genes PaPhy1 and PaPhy2. The present application first discovers that the filamentous fungus Podospora anserina with gene knockout of phytochrome genes PaPhy1 and / or PaPhy2 can express superoxide dismutase and peroxidase more efficiently, which is of great significance for the high-efficient expression and preparation of superoxide dismutase and peroxidase.

The invention relates to the technical field of biology, and discloses an efficient extraction process of SOD in silphium perfoliatum, which comprises the following steps: mixing a silphium perfoliatum superoxide dismutase crude extract, polyethylene glycol and a first salt, and adjusting the pH value to form an aqueous two-phase system to enrich SOD; adding polysaccharide hydrolase and protease into the system to carry out in-situ enzymolysis so as to degrade macromolecular impurities; after the enzymolysis is finished, adding a second salt for competitive distribution, and selectively removing the added functional enzyme to a salt phase; and finally, separating and collecting a supernatant phase rich in superoxide dismutase. By integrating aqueous two-phase extraction, in-situ enzymolysis and competitive distribution into a single reaction system, a tedious intermediate separation unit in a traditional process is replaced, and by continuous and targeted removal of endogenous macromolecular impurities and exogenous functional enzymes, the purity and specific activity of a final product are remarkably improved, so that the method is suitable for industrial production. And the biological activity of SOD is effectively protected by the mild separation conditions in the whole process.

The invention discloses application of polydatin in preparation of a medicine for relieving hepatotoxicity caused by zearalenone, and belongs to the technical field of biological medicine. The invention reveals that polydatin inhibits hepatotoxicity caused by zearalenone through targeted xanthine oxidase for the first time, so that the toxicity of zearalenone to an organism is relieved. Polydatin can be directly combined with xanthine oxidase to inhibit the catalytic activity of xanthine oxidase, so that the oxidative stress injury of the liver after ZEA exposure is reduced, meanwhile, the content of malondialdehyde (MDA) in the liver can be remarkably reduced, the activity of superoxide dismutase (SOD) is improved, the liver oxidation-antioxidant balance is recovered, and liver cells are protected from oxidative injury of ZEA. The invention provides a new scientific basis and development direction for application of polydatin in the fields of mycotoxin detoxification, oxidation resistance, liver protection and the like.

The invention provides two-dimensional bimetal organic framework nano-enzyme hydrogel as well as a preparation method and application thereof, and belongs to the technical field of biological materials. According to the preparation method, a FeCuMOF bimetal organic framework is adopted as a nano-enzyme, tannic acid and lipoic acid are used for constructing a composite hydrogel system with antibacterial and antioxidant capacities, and the composite hydrogel is combined with FeCuMOF to form the two-dimensional bimetal organic framework nano-enzyme hydrogel. The two-dimensional bimetal organic framework nano-enzyme hydrogel disclosed by the invention has antibacterial and anti-biofilm properties, and can be used for removing various ROS (Reactive Oxygen Species) by simulating superoxide dismutase and the capability of removing ABTS free radicals thereof. In addition, proinflammatory cytokines can be reduced, and anti-inflammatory cytokines can be increased, so that periodontitis inflammation is relieved, and tissue regeneration is accelerated. The two-dimensional bimetal organic framework nano-enzyme hydrogel disclosed by the invention is used as a multifunctional nano-enzyme hydrogel slow-release platform, and an ideal strategy is provided for treating periodontitis.

The invention relates to the technical field of agriculture, and discloses a synergistic technology for repairing soil and promoting plant stem cells by using modified amino active groups. Comprising the following steps: modifying an amino acid compound, namely modifying active groups in amino acid and fulvic acid, so that the active groups have a synergistic effect with cellulase, hemicellulase, urease, phosphatase, chitinase, beta-glucanase, superoxide dismutase, peroxidase and catalase; through the strong chelation of the modified amino active groups and heavy metal ions in soil, the bio-availability and mobility of heavy metals can be effectively reduced, for example, the chelation removal rate of cadmium, lead and other heavy metals can reach 40% or above, the heavy metal contaminated soil is repaired, the soil structure is improved, the content of organic matters in the soil is increased, and the pH value of the soil is adjusted; the acidified or alkalized soil is gradually restored to a pH range suitable for plant growth, and the water and fertilizer retention capability of the soil is improved.

Complexes of polyoxometalate (POM) cluster anions and metal oxide nanocrystal (NC) cores (POM-NC) with tunable NC surface protonation are disclosed. These protonated POM-NC complexes enable precise control over catalytic properties, enhancing catalytic activity under near-neutral pH conditions while improving stability and solubility across various applications. The protonated POM- metal oxide complexes facilitate the activation of airborne O2 under ambient conditions and the production of reactive oxygen species (ROS), including hydroxyl radicals, superoxide, and peroxides. In certain catalytic processes, these complexes mimic enzymatic functions, such as oxidase, peroxidase, catalase, and superoxide dismutase activity, making them valuable for various biological, therapeutic, chemical and environmental applications such as applications related to oxidative catalysis and ROS scavenging.

The invention discloses a preparation method and application of a multifunctional layered nanosheet. The biomimetic hydrogel is prepared through a hydrothermal method, folic acid (FA) is modified on the surface of the biomimetic hydrogel, and the biomimetic hydrogel has the capacity of targeted recognition of pro-inflammatory phenotype macrophages, has the biomimetic activity of multiple enzymes such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) and can effectively remove ROS; meanwhile, a succinate dehydrogenase (SDH) inhibitor dimethylmalonic acid (DMM) is loaded in an intercalation mode of the nano material, SDH activity is inhibited in a slow release mode, ROS outbreak caused by mitochondrial reverse electron transfer (RET) is blocked from the source, then a mitochondrial autophagy pathway mediated by BNIP3-LC3B is started, and dynamic and steady state recovery of mitochondria is achieved. The system finally realizes macrophage mitochondrial function reconstruction, oxidation environment improvement and transfer of healthy mitochondria to osteoblasts, so that the osteogenic differentiation capability is recovered, and the effect of treating osteoporosis is achieved.

The invention discloses a compound enzyme preparation and a preparation method thereof, and relates to the technical field of enzyme preparations. The compound enzyme preparation is at least prepared from the following components in parts by weight: 10 to 30 parts of xylanase, 15 to 35 parts of modified cellulase, 8 to 20 parts of glucanase, 5 to 15 parts of protease, 5 to 18 parts of amylase, 3 to 12 parts of pectinase, 5 to 25 parts of phytase and 15 to 40 parts of modified superoxide dismutase compound microspheres. The compound enzyme preparation provided by the invention is added into daily ration of ruminants, so that the apparent digestibility and growth performance of the ruminants on nutrients can be improved, meanwhile, safer and better meat quality can be obtained, the meat taste is improved, and higher economic value is obtained.

This invention relates to the field of microbial technology, specifically to a strain of *Lactobacillus rhamnosus* and its uses. The preservation number of the *Lactobacillus rhamnosus* strain is CGMCC No. 25682. The *Lactobacillus rhamnosus* strain of this invention exhibits excellent free radical scavenging, oxidative stress response, antioxidant, and anti-aging capabilities. It can increase the levels of superoxide dismutase, glutathione peroxidase, and glutathione, decrease malondialdehyde levels, and improve the scavenging rate of hydroxyl radicals and DPPH radicals. It can produce DPP-4 and α-glucosidase inhibitors and stimulate NCI-H716 cells to produce GLP-1, demonstrating potential for blood glucose regulation. The *Lactobacillus rhamnosus* strain of this invention can also be used to ferment and produce fermented dairy products, fermented oat products, and other fermentation products.

The invention discloses an engineering strain for soluble expression of human Cu / Zn superoxide dismutase as well as a construction method and application of the engineering strain, and relates to the technical field of biology. The construction method of the engineering strain comprises the following steps: cloning a coding gene of the human Cu / Zn superoxide dismutase into an expression vector to construct a recombinant plasmid; transforming the recombinant plasmid into escherichia coli competent cells to construct the engineering strain; the nucleotide sequence of the coding gene is as shown in SEQ ID NO. 2. The invention provides a new engineering strain for improving the expression abundance of the human Cu / Zn superoxide dismutase in escherichia coli and the proportion of a soluble expression part, and has important industrial application value.

The invention belongs to the technical field of preparation of peptides, and particularly relates to camel placenta hydrolysate as well as a preparation method and application thereof. According to the preparation method of the camel placenta hydrolysate and the preparation method of the camel placenta hydrolysate provided by the invention, the camel placenta hydrolysate has remarkable scavenging activity on DPPH, ABTS, hydroxyl free radicals and iron ions, so that the antioxidant activity is exerted; in a fruit fly acute oxidative damage model, the camel placenta hydrolysate can prolong the survival time of fruit flies in an oxidative stress environment; in a normal fruit fly model, the camel placenta hydrolysate can prolong the life of normal aging fruit flies, improve the action ability of the aging fruit flies and play an anti-aging role by improving the activity of in-vivo antioxidant enzymes such as total superoxide dismutase, copper-zinc superoxide dismutase and catalase and reducing the malondialdehyde level on the premise of not reducing the food intake of the fruit flies. The camel placenta hydrolysate can be used as an ideal auxiliary antioxidant and anti-aging medicine and health food raw material.

Provided herein are compositions, systems, kits, and methods for treating a patient with a thromboembolism by administering nanoconjugates comprising nanoparticles encapsulating and / or conjugated to: i) tissue-type plasminogen activator (tPA), and ii) at least one antioxidant enzyme selected from the group consisting of: superoxide dismutase, glutathione peroxidase, glutathione reductase, and a catalase.

The invention is suitable for the technical field of pesticide residue detection, and provides a method for distinguishing and detecting pesticide based on copper-platinum bimetallic nano-enzyme. According to the method, a colorimetric sensing array with three sensing channels is constructed by synthesizing a copper-platinum bimetallic nano-enzyme (CuPtNPs) with oxidase-like activity, laccase-like activity and superoxide dismutase-like activity. A fingerprint spectrum is obtained based on specific effects of different pesticides and enzyme-like activities, and qualitative distinguishing and quantitative detection of various pesticides are realized by combining a machine algorithm. According to the method, nine pesticide residues can be measured at a time, six of the nine pesticide residues can be quantitatively analyzed, and the problem that a traditional method only can be used for single detection is solved. A strategy of constructing three channels based on a single nano-enzyme receptor CuPtNPs has the remarkable advantages of simple synthesis process, convenient operation flow and low detection cost, is good in applicability in actual agricultural product samples such as Chinese cabbages and apples, and provides an efficient solution for high-throughput detection of pesticide residues.

The invention discloses preparation of an antibacterial copper-based nano-enzyme fiber dressing capable of promoting wound healing, and belongs to the technical field of medical use. According to the method, 5, 6-dimethylbenzimidazole and copper salt are used as raw materials, the copper-based nano-enzyme is synthesized through a hot bath reaction method of a solvent, reactants are uniformly dispersed in a solution, and the defects that traditional nano-enzyme element components are not uniform, and active sites are low in density are overcome. The prepared copper-based nano enzyme has a unique metal active center, and has oxidase-like activity, catalase-like activity and superoxide dismutase-like activity. According to the copper-based nano-enzyme Cu-NPs II, the activity of oxidase can be achieved without hydrogen peroxide, active oxygen can be generated to achieve the bactericidal effect, when excessive ROS is generated, superoxide dismutase-like activity of the copper-based nano-enzyme Cu-NPs II converts the excessive ROS into H2O2, and due to the fact that the copper-based nano-enzyme Cu-NPs II has catalase-like activity and can decompose H2O2 into water and oxygen, the copper-based nano-enzyme Cu-NPs II has good microenvironment adjusting capacity.

The invention relates to the technical field of biological medicine, and discloses a RuCDzyme nano-enzyme preparation method, which comprises: S1, dissolving carbon quantum dots (CDs) and ruthenium metal salt in a solvent A to obtain a mixed solution; s2, adjusting the pH value of the mixed solution to be alkaline, adding a sodium borohydride solution into the mixed solution, and stirring to react for 3-6 hours; after the reaction is finished, dialyzing reaction liquid to remove unreacted reactants and the solvent A, and performing freeze-drying treatment on the dialyzed reaction liquid to obtain the RuCDzyme nano-enzyme. The invention also discloses a preparation method of the TPP-coated RuCDzyme nano-enzyme, which comprises the following steps: adding EDC and NHS into a TPP-COOH solution, stirring and reacting for 30-60 minutes at 25-30 DEG C, then adding the RuCDzyme nano-enzyme into a reaction system, continuing stirring and reacting for 16-24 hours, dialyzing a reaction solution after the reaction is finished, removing unreacted reactants in the reaction solution, and freeze-drying the dialyzed reaction solution to obtain the TPP-coated RuCDzyme nano-enzyme. The RuCDzyme nano-enzyme and the TPP coated RuCDzyme nano-enzyme prepared by the invention have similar superoxide dismutase activity, similar catalase activity and similar glutathione peroxidase activity, and can efficiently remove active oxygen to achieve the effect of preventing, relieving or / and treating renal injury.

The application discloses a superoxide dismutase biological anti-stress nutrient fertilizer based on plant extraction and a production method thereof. The fertilizer contains, in percentage by mass, 1.0-10.0% of superoxide dismutase, 1.0-5.0% of a plant anti-stress inducing factor complex, 0.1-2.0% of organic chelated trace elements, 5.0-15.0% of a phosphorus-potassium enhancing component, 0.5-5.0% of a natural protective agent, 3.0-10.0% of a penetrating agent, 0.5-15.0% of a microbial metabolic synergist, 0.1-5.0% of a pH buffer component, 0.01-0.5% of a plant-derived preservative, and the balance of water. The superoxide dismutase is preferably derived from corn germ or spinach extract. The production method comprises the steps of enzyme activity component preparation, water phase base preparation, anti-stress function phase preparation, step-by-step mixing and primary stabilization, final product integration and secondary stabilization, and the like. Through scientific compounding and synergy of various biological active components, the application significantly enhances the resistance of crops to drought, saline-alkali, high and low temperature and the like, and promotes growth and quality improvement.

The invention relates to the technical field of microorganisms, and discloses a talaromyces funiculi LD-YGH31 and an application of the talaromyces funiculi LD-YGH31 in salt tolerance. The strain is separated from the root system of halophyte, and the preservation number of the strain is CCTCC NO: M 20251815. After the strain is cultured, the strain and plants are co-cultured, and the growth performance and stress resistance of the plants under salt stress can be remarkably improved. Experiments prove that when the strain and tomato seedlings are co-cultured, under the stress condition of 200 mM NaCl salt, the fresh weight and plant height of the tomato seedlings can be remarkably improved, the activity of superoxide dismutase and peroxidase is improved, the content of malondialdehyde and the relative conductivity are reduced, and the K < + > / Na < + > steady state in plant roots and leaves is maintained. The strain is novel, the application method is simple and easy to implement, and the strain can be developed into a microbial fertilizer for saline-alkali soil crop planting and has remarkable agricultural application value.