425 results about "Dismutase" patented technology

The invention discloses a compound enzyme preparation for improving the absorption and utilization rate of a fertilizer and a preparation method thereof, and belongs to the technical field of enzyme preparations. The compound enzyme preparation is prepared from the following components in parts by weight: 100 parts of purified water, 6 to 8 parts of modified cellulase, 10 to 12 parts of immobilized protease, 10 to 12 parts of phosphatase, 10 to 14 parts of urease, 8 to 10 parts of nitrate reductase, 10 to 12 parts of pectinase, 8 to 10 parts of chitinase, 6 to 8 parts of xylanase, 10 to 12 parts of beta-glucosidase, 10 to 12 parts of superoxide dismutase and 4.5 to 6 parts of a microbial agent, the modified cellulase is obtained by chemically modifying cellulase; the immobilized protease is prepared by the following steps: adsorbing activated and coupled modified polylactic acid and a protease solution, crosslinking with glutaraldehyde, filtering, washing and drying. The compound enzyme preparation improves the fertilizer absorption and utilization rate of plants.

The invention relates to a composite hydrogel dressing for promoting wound healing as well as preparation and application thereof, and belongs to the technical field of biological medicines. The hydrogel disclosed by the invention is prepared by mixing gelatin, oxidized dextran and metal ion modified metal-organic framework compounds (MOF-Ag and MOF-Cu). In the mixed solution, amino groups in the gelatin and aldehyde groups of the oxidized dextran are subjected to a Schiff base cross-linking reaction, and the composite hydrogel is formed after standing. The loaded MOF-Ag has antibacterial and anti-biofilm effects on staphylococcus aureus and escherichia coli, and the loaded MOF-Cu has similar superoxide dismutase activity and similar glutathione peroxidase activity, so that excessive active oxygen can be removed, and inflammatory response can be relieved. The obtained hydrogel has good biocompatibility, proper mechanical properties and biological adhesion, stable rheological properties, excellent antibacterial and anti-inflammatory functions and a certain hemostatic function.

The invention provides a nano transdermal delivery system based on superoxide dismutase, the nano transdermal delivery system is of a capsule composite structure of a small molecule active component-superoxide dismutase-polymer shell layer, the small molecule active component is loaded in superoxide dismutase, the surface of the superoxide dismutase is coated by the polymer shell layer, and the superoxide dismutase is loaded in the capsule composite structure. The diameter of the superoxide dismutase-based active ingredient transdermal delivery system is 20-100 nm, and the thickness of the polymer shell layer is 7.5-52.5 nm. In the delivery system, the polymer shell layer provides a protection effect for the superoxide dismutase and the micromolecular functional substances with the anti-oxidation effect, the capsule composite structure is in a nanogel form, and the nanogel not only promotes the penetration depth of the capsule, but also reduces the irritation effect of the capsule on the skin. The raw materials are biologically friendly, the preparation method is simple, the yield is high, and industrial large-scale production can be realized.

The invention provides a preparation method of medicinal liquor and application of the medicinal liquor in preparation of antioxidant and anti-aging products, and relates to the technical field of medicinal preparations. The preparation method comprises the following steps: (1) crushing; (2) extracting; (3) standing and separating; and (4) freezing and filtering to obtain the medicinal liquor. The medicinal liquor is obtained by extracting the traditional Chinese medicine components through the steps of crushing in batches, mixing and extracting, standing, freezing and separating the supernate and the suspension in different separation modes, and the medicinal liquor can better improve the space exploration capability of a mouse aged due to D-galactose, can reduce malondialdehyde MDA, and can improve the immunity of the mouse. The activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) is improved, and the obvious anti-oxidation and anti-aging effects are achieved.

The invention discloses a tumor cholesterol metabolism regulation microneedle patch and a preparation method, the microneedle patch comprises a needle tip and a backing which are connected, the needle tip comprises a needle tip body and nanoparticles loaded on the needle tip body, the nanoparticle is a manganese ion-doped organic metal framework, the outer layer of the manganese ion-doped organic metal framework is modified with a targeting agent, cholesterol oxidase and superoxide dismutase are carried on the manganese ion-doped organic metal framework, the targeting agent can target a CD44 receptor, and the organic metal framework can carry drugs. The targeted drug can enter tumor cells in a targeted mode, superoxide dismutase catalyzes superoxide anions overexpressed in the tumor cells to generate H2O2 and O2, O2 needed by catalysis is provided for cholesterol oxidase, cholesterol at the tumor site is consumed by the cholesterol oxidase, accumulation of 7-DHC is reduced, meanwhile H2O2 can be generated, and the cholesterol oxidase can be used for catalyzing the tumor site. H2O2 is catalyzed by manganese ions through a Fenton-like reaction to generate OH to induce tumor cells to generate ferroptosis, so that ferroptosis-immune synergistic treatment is realized.

The invention relates to the field of biomedical materials, and particularly discloses a manganese dioxide nano-enzyme for treating inflammatory bowel disease as well as a preparation method and application of the manganese dioxide nano-enzyme. The preparation method of the manganese dioxide nano-enzyme comprises the following steps: uniformly mixing an organic solution containing manganese nitrate hexahydrate with a montmorillonite aqueous solution to obtain a mixed reaction solution; the pH value of the mixed reaction solution is adjusted to be under the alkaline condition through alkali liquor, a synthetic reaction is carried out, then centrifugation and washing are carried out, the montmorillonite-loaded manganese dioxide nano-enzyme is obtained, and the manganese dioxide nano-enzyme is of a lamellar structure with the length being 20-400 nm. The manganese dioxide nano enzyme has excellent activity similar to superoxide dismutase, catalase and glutathione peroxidase; meanwhile, the compound also has the capability of removing various free radicals such as DPPH and ABTS, can be used for treating the inflammatory bowel disease, and has a wide application prospect.

The present invention relates to magnetic, catalytically active, and radiosensitizing nanoparticles (NanoXcats) and the method for their production. A method is disclosed for the production of functionalized greigite nanoparticles with a narrowband size distribution (10-30 nm) for use in multimodal cancer therapy. NanoXcats are superparamagnetic Fe3S4 nanoparticles whose surfaces are equipped with polar functional groups. The surface structures of NanoXcats are suitable for conjugation with, or for covalent or electrostatic binding of, active substances, which may be cancer drugs, cell growth inhibitors, or NO donors. Due to the EPR effect, NanoXcats are preferentially internalized by tumor cells. The targeted accumulation of NanoXcats in tumor tissue is achieved by an external inhomogeneous magnetic field. NanoXcats exhibit enhanced catalytic activity in tumor cells.The catalytic activity of intracellular NanoXcats can be triggered, controlled, and enhanced by low-dose X-rays. Surface-bound drugs are additionally activated in tumor cells by the catalytic activity of NanoXcats. NanoXcats act as superoxide dismutase, nitric oxide synthase, and Fenton catalyst. They catalyze, for example, reactions of the superoxide anion radical to hydrogen peroxide, of L-arginine to NO, and of hydrogen peroxide to the hydroxyl radical.

The invention discloses bacillus subtilis ZX-5 for promoting growth of gleditsia sinensis under salt stress and application of the bacillus subtilis ZX-5, and relates to the technical field of microorganisms. The invention discloses bacillus subtilis ZX-5 for promoting growth of gleditsia sinensis under salt stress, and the bacillus subtilis ZX-5 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.31648. The result of the embodiment shows that the bacillus subtilis ZX-5 promotes the ground diameter growth rate and seedling height growth rate of the gleditsia sinensis to be improved under salt stress, and promotes the total root length and root tip number of the gleditsia sinensis root system to be increased; the surface area of the gleditsia sinensis root is increased under the stress of 150mM salt. The activities of Chinese honey locust superoxide dismutase, peroxidase, catalase and ascorbic acid peroxidase are promoted to be enhanced under the stress of salt; the contents of ascorbic acid and reduced glutathione in the gleditsia sinensis are promoted to be increased under the stress of salt; the content increase of soluble protein, soluble sugar and free proline in the gleditsia sinensis is promoted.

The invention discloses parabacteroides faecium capable of relieving senescence and application of the parabacteroides faecium, and belongs to the technical field of microorganisms and the technical field of medicines. Animal experiments prove that Parabacteroides vervae CCFM1428 has the effects of improving the weakness index of a mouse, increasing peroxide dismutase in the liver of the mouse, reducing malonaldehyde, increasing the level of glutathione peroxidase (GSH-px), increasing the level of catalase, reducing an inflammatory factor IL-6, reducing an inflammatory factor TNF-alpha and increasing the level of acetylcholine in the brain of the mouse, and has the effects of improving the weakness index of the mouse, increasing the liver peroxide dismutase of the mouse, reducing malonaldehyde, increasing the level of glutathione peroxidase (GSH-px) and the level of catalase. The gripping force of the mouse is improved. The strain disclosed by the invention has a huge application prospect in preparation of products for relieving senescence.

The invention provides an antioxidant enzyme-containing composition and a preparation method thereof, and relates to the technical field of enteritis treatment. The preparation method comprises the following steps: S1, collecting various component materials for preparing the antioxidant enzyme-containing composition: collecting various raw materials for preparing glucan, fructo-oligosaccharide, galactooligosaccharide, superoxide dismutase, vitamin C, bunge cherry seed powder, cassia seed powder, turmeric, sorbitol, hydroxypropyl methyl cellulose, silicon dioxide and magnesium stearate; according to the invention, a matched powder bagging machine is adopted to realize full-automatic unattended operation to automatically complete the large bag filling and sealing step in the steps S4-S10, vacuumizing is performed firstly and then sealing is performed, and the storage space is compressed by vacuumizing, so that the condition that the bag is expanded and broken by internal air pressure in the transfer and storage process is not easy to cause; in the bag sealing process, the phenomena of high hot melting efficiency and high sealing speed are achieved by adopting a bag strip guiding and rolling hot melting mode.

The invention provides a coordination nano assembly of chiral cyclopeptide and manganese ions as well as a preparation method and application of the coordination nano assembly. According to the method, a manganese superoxide dismutase protein structural domain and a unique tumor microenvironment of melanoma high tyrosinase are combined, and polypeptide sequences L-YD-hL-DD-h, L-YL-HL-DL-H and D-yD-hD-dD-h with different chirality are designed for the first time; the chirality of the polypeptide is regulated and controlled, the in-vivo circulation stability and the cell entry efficiency of the nano assembly are improved, the coordination self-assembly of manganese and different chiral cyclopeptides is realized, and the in-situ oxidation of an in-vivo tumor microenvironment is simulated for photo-thermal therapy and cell membrane coating. The method disclosed by the invention is simple, mild in experimental condition and easy to operate, the prepared cell membrane coated nano assembly not only has long circulation stability and high cell entry efficiency, but also can realize mild photo-thermal therapy through in-situ oxidation, releases manganese ions to activate a CGAS-STING pathway for immunotherapy of tumors, and has a wide application prospect. The potential application value is realized in the field of combined treatment of tumors.

The invention discloses application of an OsLOX1 gene in regulation and control of drought resistance and seed vigor of rice, and relates to the technical field of plant genetic engineering. According to the invention, the drought stress tolerance of the mutation line with the OsLOX1 knocked out through CRISPR / Cas9 is lower than that of a wild type line, and along with the increase of the content of hydrogen peroxide (H2O2) and malondialdehyde (MDA) and the reduction of the expression quantity of antioxidant enzyme related genes. In addition, it is found that compared with a wild type, the service life of rice seeds is shortened by knocking out OsLOX1, meanwhile, the content of H2O2 and MDA is increased, and the activity of antioxidant enzyme superoxide dismutase (SOD) and catalase (CAT) is reduced; the method can be used for breeding storage-resistant and drought-resistant rice varieties; important gene resources are provided for cultivating drought-resistant rice varieties and prolonging the service life of seeds.

The invention belongs to the technical field of catalytic materials, and particularly relates to high-entropy alloy nanoparticles used as a biocatalyst and application of the high-entropy alloy nanoparticles. The PtFeCuCoNi high-entropy alloy nano-particles with pH adaptability are developed through a one-step solvothermal synthesis method, it is found that the PtFeCuCoNi high-entropy alloy nano-particles can serve as potent oxidase, can generate reactive oxygen species (ROS) and effectively kill bacteria such as MRSA under the acidic condition, and can play the functions of superoxide dismutase and catalase under the neutral condition, so that the PtFeCuCoNi high-entropy alloy nano-particles with the pH adaptability can be used for effectively killing bacteria such as MRSA. Active oxygen in cells is efficiently removed, oxidative stress is relieved, and the cell protection effect is achieved.

The invention belongs to the technical field of microorganisms, and particularly relates to a lactobacillus plantarum (Lactobacillus plantarum) and an application of the lactobacillus plantarum. The preservation number of the lactobacillus plantarum is CGMCC (China General Microbiological Culture Collection Center) NO.24756. The lactobacillus plantarum has good DPPH (1, 1-diphenyl-2-picrylhydrazyl) scavenging capacity, hydroxyl radical scavenging capacity and reducing capacity, and can remarkably reduce the level of malondialdehyde (MDA) as an oxidative stress marker in serum and liver of an oxidative stress body, the pathological damage level of liver tissue and the level of inflammatory factors in serum; the level of an antioxidant substance glutathione (GSH) in serum and liver of an oxidative stress body and the activity of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) in the serum and liver can be remarkably improved. In addition, fire extinguishing bacteria of the lactobacillus plantarum have more excellent antioxidant activity than viable bacteria.

The invention provides a composition with a function of stabilizing skin enzyme activity as well as a preparation method and application of the composition, and the composition is prepared from the following components in parts by mass: 0.001 to 0.01 part of superoxide dismutase, 0.001 to 0.05 part of nicotinamide adenine dinucleotide, 0.11 to 12 parts of a polypeptide compound a and 0.005 to 0.5 part of a polypeptide compound b. The invention also provides a preparation method of the composition and application of the composition in preparation of skin care products for improving skin enzyme activity, repairing skin and resisting aging. The composition can be prepared into emulsion, solution, paste, gel or spray. The composition can effectively improve the enzyme activity of skin, improve the texture and appearance of the skin and repair the skin, and is beneficial to delaying the aging of the skin at the same time.

The invention discloses a method for evaluating and screening salt tolerance of triticale germplasm resources in a germination stage, and belongs to the technical field of evaluation and screening of plant salt tolerance, salt stress concentration screening: triticale seeds are treated by NaCl solutions with different concentrations, indexes such as survival rate, plant height and overground part fresh weight are measured, the NaCl solution with the concentration of 200 mmol. L <-1 > is determined as the salt tolerance evaluation appropriate concentration, and the salt stress concentration is determined as the salt tolerance evaluation appropriate concentration. Salt tolerance evaluation test: carrying out salt stress treatment by adopting a 200mmol. L <-1 > NaCl solution, determining phenotypic indexes such as survival rate, plant height, fresh weight of overground part, root length and chlorophyll content, and physiological and biochemical indexes such as catalase, peroxidase and superoxide dismutase activity and malondialdehyde content, and carrying out principal component analysis, membership function analysis and clustering analysis to obtain a salt tolerance evaluation result. Calculating a salt tolerance comprehensive evaluation value, and screening salt tolerance triticale germplasm resources.

The invention discloses marasmius albus DCY6478 as well as a culture medium and application thereof, and belongs to the technical field of microorganisms. The invention discloses a Marasmilla sp. DCY6478 strain, and the preservation number of the Marasmilla sp. DCY6478 strain is GDMCC (China General Microbiological Culture Collection Center) No.65883. The Marasmilla sp. Experimental verification results show that the culture medium formula most suitable for growth of the white marasmius is prepared from the following components in percentage by weight: 40% of apple sawdust, 36% of corncob, 14% of cottonseed hull, 1% of lime and 1% of gypsum; the fermentation liquor is verified to have an inhibition effect on a plurality of pathogenic bacteria; the DPPH free radical scavenging capacity of the fermented hyphae is verified, the superoxide dismutase activity and catalase activity are both higher than those of sporocarp, and the fermented hyphae can be used as a natural antioxidant source; it is verified that fermented hyphae or sporocarp of the strain have the capacity of secreting amino acid and polysaccharide and producing crude protein, and a new raw material selection is provided for the industries of food, medicine and the like.

Provided is a superoxide dismutase-based nanoscale transdermal delivery system, which is a capsule composite structure consisting of a small molecule active ingredient, superoxide dismutase, and a polymer coating. The small molecule active ingredient is loaded in the superoxide dismutase, and the surface of the superoxide dismutase is coated with the polymer coating. The superoxide dismutase-based active ingredient transdermal delivery system has a diameter of 20-100 nm, and the polymer coating has a thickness of 7.5-52.5 nm. In the delivery system, the polymer coating protects the superoxide dismutase and the small molecule functional substance having an antioxidant effect, and the capsule composite structure is in the form of nanogel. The nanogel not only promotes the penetration depth of the delivery system, but also mitigates the stimulation of the delivery system to the skin. The delivery system features biofriendly starting materials, simple preparation method, and high yield, and thus can be produced in large scale industrially.

The invention discloses a method for preparing recombinant human copper-zinc superoxide dismutase. The method comprises the following steps: (a) designing and synthesizing a gene sequence for coding the recombinant human copper-zinc superoxide dismutase; (b) cloning the gene sequence into an expression vector to construct a recombinant expression vector; (c) transforming the recombinant expression vector into an expression host cell to obtain a recombinant strain; (d) inducing the expression host cell to express the recombinant human copper-zinc superoxide dismutase under proper conditions; and (e) collecting an expression product, and carrying out multi-step purification to obtain the high-purity recombinant human copper-zinc superoxide dismutase. According to the superoxide dismutase provided by the invention, the expression quantity and activity of recombinant protein are remarkably improved, the purification process is simplified, the production cost is reduced, and meanwhile, the high-quality standard of a product is ensured.

The invention belongs to the technical field of aquaculture, and particularly relates to application of resveratrol in culture of penaeus vannamei boone, which is characterized in that the application dosage and mode of the resveratrol are that the penaeus vannamei boone is fed according to the weight proportion of 0.033-0.067 per thousand of the total weight of the penaeus vannamei boone everyday. The resveratrol is used for improving the immune function of penaeus vannamei boone, improving the survival rate, increasing the body weight growth rate and counting immune cells. The resveratrol is also used for improving the anti-oxidative stress capability of the South America white, including improving the superoxide dismutase activity of the shrimp body, and / or improving the shrimp hepatopancreas lysozyme activity of the shrimp body, and / or improving the total anti-oxidative capability of the shrimp body. The resveratrol can enhance the immune epidemic prevention level of the penaeus vannamei population on stress sources such as vibrio infection, reduce the oxidative stress risk of the penaeus vannamei population and avoid the expansion of breeding loss caused by group stress.

The invention belongs to the technical field of enzyme engineering, and particularly relates to a codon optimized superoxide dismutase AfSOD gene and application thereof in degradation of aflatoxin B1 and zearalenone. Under the conditions that the pH is 7, the temperature is 40 DEG C and the enzyme addition amount is 10 micrograms, the degradation rate of the AFB1 reaches 99.08% within 24 hours, and the degradation rate is kept to be 80% or above within 40-80 DEG C; under the conditions that the pH is 8, the temperature is 60 DEG C and the enzyme addition amount is 20 micrograms, the degradation rate of ZEN reaches 85.1% in 24 hours, and the degradation rate is kept to be 80% or above within 50-80 DEG C; the result shows that AfSOD is a toxin degrading enzyme which can tolerate a certain degree of high temperature; the degradation rates of AFB1 and ZEN in polluted peanut powder or corn powder are 73.7% and 88.2% respectively; the invention provides a new candidate for detoxification of AFB1 and ZEN in food or feed, and has a good application prospect.

The invention relates to the technical field of biological medicine. The invention aims to provide an application of Mn3O4 nanoparticles in relieving intervertebral disc degeneration. The manganous-manganic oxide nanoparticles Mn3O4NPs are added into an interventional drug for treating intervertebral disc degeneration as a nano-enzyme. According to the invention, Mn3O4 nanoparticles are used as a nano active catalytic enzyme, have multienzyme catalytic activity similar to superoxide dismutase, catalase and glutathione peroxidase, can effectively remove reactive oxygen species (ROS) and regulate ECM metabolism, significantly reduce cell apoptosis, delay cell senescence, inhibit inflammation and enhance autophagy, and can be used for preparing a medicine for treating hyperplasia. Therefore, annulus fibrosus AF repair in intervertebral disc degeneration treatment is promoted. The Mn3O4 nano particles have good biocompatibility, metabolites of the Mn3O4 nano particles can be used for other physiological processes, and no toxic or side effect is generated in in-vivo circulation. Meanwhile, the enzyme is stable in low-oxygen and low-pH adverse microenvironment of the intervertebral disc, and has in-vivo stability which is not possessed by other active catalytic enzymes.

The invention belongs to the technical field of molecular biology, and particularly relates to application of an OsSCL25 gene in regulating and controlling salt stress resistance of rice. Under the stress of salt, the survival rate, chlorophyll, fresh weight and dry weight of strain seedlings of the overexpressed OsSCL25 gene are higher than those of a wild type, the content of superoxide dismutase, peroxidase and malondialdehyde is lower than that of the wild type, and the content of proline is remarkably higher than that of the wild type and a mutant. The soluble sugar content of the wild type, the soluble sugar content of the OsSCL25 mutant and the soluble sugar content of the overexpression system are not obviously different under normal treatment, and the soluble sugar content of the overexpression system is obviously higher than that of the wild type and the soluble sugar content of the gene mutant after salt stress, so that the OsSCL25 gene participates in rice salt tolerance regulation. After the gene is over-expressed, the salt tolerance of the rice is obviously improved, so that a rice material suitable for saline-alkali soil planting improvement can be cultivated by utilizing the OsSCL25 through a biotechnology, and a new thought is provided for improving the planting rate of the rice in the saline-alkali soil.

The invention relates to a modified superoxide dismutase pH response type nano-liposome and a synergistic anti-photoaging after-sun repair composition capable of achieving accurate targeting. The modified superoxide dismutase pH response type nano-liposome is obtained by modifying a superoxide dismutase nano-liposome with hyaluronic acid, and the modified superoxide dismutase pH response type nano-liposome has the advantages that the modified superoxide dismutase pH response type nano-liposome can be accurately targeted by the aid of the synergistic anti-photoaging after-sun repair composition; the superoxide dismutase nano-liposome is of a core-shell structure, a core layer is superoxide dismutase, a shell layer is a ternary composite liposome, and the ternary composite liposome is formed by compounding HSPC, DSPC and cholesterol. By constructing a pH response type nano delivery system, the pH drop characteristic of an ultraviolet damage area can be accurately recognized, controlled release of SOD at a target site is triggered, O2 <-> and H2O2 are synchronously removed, an oxidative stress chain reaction is blocked, and in cooperation with the inhibition of tetrahydrocurcumin on a tyrosinase signal channel and the anti-inflammatory repair effect of the aloe extract, the anti-inflammatory repair effect of the aloe extract on the tyrosinase signal channel is achieved. A three-dimensional action network of targeted removal-activity regeneration-barrier reconstruction is formed, and the photoaging prevention and treatment effect is improved.

The present invention relates to intracellular copper zinc superoxide dismutase inhibitors for protecting a subject from damage caused by radiation outbreak. The invention also relates to kits comprising SOD1 inhibitors and radiosensitizers for cancer cells, to the in vitro use of SOD1 inhibitors for protecting non-cancer cells from ionizing radiation, and to methods, compositions and uses related thereto.

The invention provides a blood purification adsorbent and a preparation method thereof, the blood purification adsorbent takes macroporous resin containing an epoxy group as a carrier, the epoxy group on the carrier reacts with polyethyleneimine, the polyethyleneimine is immobilized on the carrier, the polyethyleneimine is taken as a spacer arm, and the epoxy group on the carrier reacts with the polyethyleneimine to prepare the blood purification adsorbent. Superoxide dismutase and lipoic acid are connected to the spacer arm through a dehydration condensation reaction, so that the superoxide dismutase and the lipoic acid are immobilized on the carrier. The blood purification adsorbent can remove superoxide, free radicals and inflammatory factors at the same time, and can effectively improve oxidative stress and microinflammation states.

The invention provides collagen modified superoxide dismutase and a preparation method and application thereof, the preparation method comprises the following steps: dissolving collagen in water, sequentially adding superoxide dismutase, EDC and NHC into the water, and stirring in a water bath heat preservation state to obtain a flowable gel sample; diluting the obtained gel sample with water, centrifuging, and collecting a lower-layer viscous liquid; and adding the centrifuged viscous liquid into a PBS (phosphate buffer solution), and dialyzing to obtain the collagen modified superoxide dismutase. Trehalose and mannitol are added into the collagen modified superoxide dismutase, the mixture is stirred and mixed evenly, the mixture is poured into a mold to be freeze-dried and extruded to be flat, and the repairing patch is obtained. The crosslinking modified superoxide dismutase obtained in the invention has good stability, and the obtained repair patch is convenient to use, can promote wound healing, and has wide application prospects and practical significance.

The present application discloses a filamentous fungal mutant strain with high-efficient expression of superoxide dismutase and its application. The filamentous fungal mutant strain of the present application is formed by gene knockout of phytochrome genes PaPhy1 and / or PaPhy2 in the wild-type filamentous fungus Podospora anserina. The filamentous fungal mutant strain of the present application is a mutant strain with gene knockout of phytochrome gene PaPhy1, a mutant strain with gene knockout of phytochrome gene PaPhy2, or a mutant strain with gene knockout of phytochrome genes PaPhy1 and PaPhy2. The present application first discovers that the filamentous fungus Podospora anserina with gene knockout of phytochrome genes PaPhy1 and / or PaPhy2 can express superoxide dismutase and peroxidase more efficiently, which is of great significance for the high-efficient expression and preparation of superoxide dismutase and peroxidase.

The invention relates to the technical field of biology, and discloses an efficient extraction process of SOD in silphium perfoliatum, which comprises the following steps: mixing a silphium perfoliatum superoxide dismutase crude extract, polyethylene glycol and a first salt, and adjusting the pH value to form an aqueous two-phase system to enrich SOD; adding polysaccharide hydrolase and protease into the system to carry out in-situ enzymolysis so as to degrade macromolecular impurities; after the enzymolysis is finished, adding a second salt for competitive distribution, and selectively removing the added functional enzyme to a salt phase; and finally, separating and collecting a supernatant phase rich in superoxide dismutase. By integrating aqueous two-phase extraction, in-situ enzymolysis and competitive distribution into a single reaction system, a tedious intermediate separation unit in a traditional process is replaced, and by continuous and targeted removal of endogenous macromolecular impurities and exogenous functional enzymes, the purity and specific activity of a final product are remarkably improved, so that the method is suitable for industrial production. And the biological activity of SOD is effectively protected by the mild separation conditions in the whole process.

The invention discloses a synthetic functional bacterial agent and application of the synthetic functional bacterial agent in salt tolerance and growth promotion of wheat, the synthetic functional bacterial agent is prepared from five strains, namely, Gordonia hydrophora, Lysinibacillus oldseyi, planomicrobium okeanokites, Luteimonas huaabiensis and Brevibacterium epidermidis, and the bacterial agent has the remarkable characteristics of no antagonism among the strains, salt and alkali tolerance, high viable count and the like, and can be applied to salt tolerance and growth promotion of wheat. The germination rate, the seedling length and the root length of the spring wheat can be remarkably increased, and the seedling length and the root length of the winter wheat are also remarkably promoted. Under the condition of salt stress, the microbial inoculum can remarkably improve the activity of catalase, peroxidase and superoxide dismutase of wheat and the total content of chlorophyll, after microbial inoculum treatment, the content of malondialdehyde and proline of wheat is remarkably reduced, and the microbial inoculum plays a role in promoting and protecting spring wheat and winter wheat in the process of resisting saline-alkali stress and has a good application prospect. The method has an influence effect on agricultural development and sustainable development in the arid region.