330 results about "Root tip" patented technology

A root canal is sequentially cleaned in sections from the crown to the apex by dividing it into three sections including an operative coronal portion, an operative middle portion and an apical portion. An operative phase corresponds with each section and specific instrumentation is used in each phase. After an opening is formed into the tooth to provide access into the root canal, the opening is preferably rectified to remove obstructions so that the anatomical root canal can be filly accessed. The pulp material is then sequentially removed from the portion of the root canal above the apical portion of the root canal with a set of instruments. The apical portion is then preferably cleaned with an irrigator or alternatively with another set of instruments. An optional set of instruments can also be used to improve the access into the apical portion such that irrigants can be delivered to the apical portion. Cleaning of the pulp material from the root canal also shapes the root canal to ease filling of the root canal with a filling material. The root canal preparation and shaping are completed while maintaining the original anatomy of the root canal. Each instrument comprises a handle connected to a file. Each file has an abrading portion and terminates at a tip. The files used to clean the portion of the root canal above the apical portion have properties which enable the abrading portion of the file to conform to the configuration of the root canal while simultaneously moving the file in a cleaning motion.

A root canal is sequentially cleaned in sections from the crown to the apex by dividing it into three sections including an operative coronal portion, an operative middle portion and an apical portion. An operative phase corresponds with each section and specific instrumentation is used in each phase. After an opening is formed into the tooth to provide access into the root canal, the opening is preferably rectified to remove obstructions so that the anatomical root canal can be fully accessed. The pulp material is then sequentially removed from the portion of the root canal above the apical portion of the root canal with a set of instruments and then from the apical portion with another set of instruments. An optional set of instruments can also be used to improve the access into the apical portion such that irrigants can be delivered to the apical portion. Cleaning of the pulp material from the root canal also shapes the root canal to ease filling of the root canal with a filling material. The root canal preparation and shaping are completed while maintaining the original anatomy of the root canal. Each instrument comprises a handle connected to a file. Each file has an abrading portion and terminates at a tip. The files used to clean the portion of the root canal above the apical portion have properties which enable the abrading portion of the file to conform to the configuration of the root canal while simultaneously moving the file in a cleaning motion.

A compact, easily transportable dental system to permit root and root tip extractions from human and animal patients including those who are unable to visit an oral surgeon or veterinarian at a traditional office. An extraction bur having a lifting ball is embedded within and stands upwardly from the root or root tip to be extracted. The extraction bur is detachably connected to and rotated into the root / tip by a bur implanting device. A bur ejector is slidable through the bur implanting device to cause the extraction bur to be released therefrom once the bur has been implanted in the patient's root / tip. A universal fulcrum tray covers the root / tip and surrounds the patient's adjacent teeth during the extraction process. The upstanding extraction bur and the lifting ball thereof project through one of a pair of slots formed in the universal fulcrum tray. An extracting instrument having a pivot surface rotates over the universal fulcrum tray to apply a lifting force to the lifting ball of the extraction bur to pull the bur upwardly and the root / tip outwardly from its root socket.

A root canal is sequentially cleaned in sections from the crown to the apex by dividing it into three sections including an operative coronal portion, an operative middle portion and an apical portion. The pulp material is then sequentially removed from the portion of the root canal above the apical portion of the root canal with a set of instruments having stainless steel files. The apical portion is then cleaned with another set of instruments that have nickel / titanium files. An optional set of instruments can also be used to improve the access into the apical portion such that irrigants can be delivered to the apical portion.

The invention discloses root tip detoxification and rapid propagation technology for purple potatoes and belongs to the technical field of plant detoxification and tissue culture rapid propagation. The method comprises the following steps of: (1) preparing a culture medium; (2) performing root tip detoxification and rapid propagation; and (3) performing multi-reverse transcription-polymerase chain reaction (RT-PCR) synchronous and rapid detection on complex virus infection of a purple potato test tube plantlet or a test tube mini-tuber and the like. In the technology, each level of culture medium is adjusted according to the characteristic of the purple potato and conventional stem tip detoxification is changed into root tip detoxification so as to simplify operation process, avoid high pollution rate and improve working efficiency. Moreover, the technology has the advantages of good inoculant differentiation, high test tube plantlet propagation speed, complete detoxification, high mini-tuber growth vigor, monthly mini-tuber reproduction rate of 4 to 6 times and mini-tuber rooting rate and transplanting survival rate of over 98 percent. The technology can be popularized in regions which are suitable for the growth of purple potatoes.

The invention belongs to the technical field of plant cultivation, and relates to a plant cultivation method, in particular to a cultivation method of broccoli. According to the greenhouse broccoli high-yield and high-efficiency cultivation technology, the disease resistant, medium-maturing and late-maturing broccoli with the long picking time is selected, seeds are sown, germination acceleration and poikilothermic treatment are carried out on the seeds, main root tips are cut off, growth of lateral roots are facilitated, and the seeds are cultivated in an open-mouthed rectangular container; meanwhile, by means of a nutrient solution, the seedling stage quality is good, the leaf color is dark green, the sound seedling index reaches more than 99%, planting is carried out when the seedling age is about 30 days, the seedling slowing time is short, the growth is fast, the ultimate capacity is high, the quality is good, the temperature inside a greenhouse can be adjusted by opening or closing large and small greenhouse thin films from the initial budding stage to the harvesting process of the broccoli particularly in autumn and winter which are low-temperature seasons, higher economic benefits can be obtained after the broccoli appears on the market before or after a festival, meanwhile, the quality of the broccoli is improved, the yield per mu can reach more than a ten thousand yuan, and the standardized pollution-free greenhouse cultivation is achieved.

The invention provides a method for making a hyacinth root tip chromosome slice, which comprises the following steps: carrying out the low-temperature preprocessing by adopting a 1g / L colchicin solution; fixing by a Carnoy fixing solution; dissociating for 10 minutes by utilizing 1mol / L hydrochloric acid in a 60 DEG C water bath and rinsing by utilizing distilled water which is added with a small amount of NaOH; cutting the root tip into two parts along the longitudinal direction; dyeing for 10 minutes by utilizing a carbor fuchsin dyeing agent; rinsing by utilizing the distilled water after dyeing; making the slice by utilizing a tabletting method; and carrying out the microscopic examination and microphotograph under an Motic microscope. The method for making the slice provided by the invention has the advantages of simpleness and convenience in operation, uniform chromosome dispersion and shallow background; the number of chromosomes is easy to count; and the chromosome number accuracy which is obtained by adopting the slice making method provided by the invention is high.

The invention discloses a method for rapid identification of Gerbera jamesonii ploidy by using flow cytometry. The method comprises steps of: preparing a unicell fluid suspension by using an extraction cracking buffer and a dyeing buffer; determining DNA fluorescence intensity of a sample by using a CyFlow Cube type of flow cytometer produced by German partec company and carrying out ploidy analysis; and comparing the abscissa positions of the sample to be measured and the contrast DNA content fluorescence intensity separation peak to identify the ploidy of the sample to be measured. A flow cytometer ploidy identification result is consistent with root tip chromosome counting ploidy. According to the method, ploidy of batch of Gerbera jamesonii macrospore regeneration plant can be screened out rapidly, and efficiency of DH colony construction and heredity atlas drafting is increased substantially, so as to accelerate a breeding process. The invention has advantages of simple sample preparation, rapid detection and stable high detection rate.

Improved compositions comprising a mixture of gutta-percha and a hydraulic material containing calcium silicate, calcium aluminate, calcium phosphate, and calcium sulfate compounds are provided. Preferably, the composition includes hydroxylapatite mineral. The composition is particularly suitable for obturating and sealing dental root canals. The composition should provide a stable barrier to bacterial and fluid leakage in the root canal system of a tooth. In addition, the composition should help promote the growth of new bone and tissue surrounding the root tip area.

The invention relates to a cultivating method of vegetable, in particular to a method of cultivating early-maturing common head cabbage strong seedlings, and belongs to the technical field of vegetable cultivation. In a seedling growing phase, a potted tray is utilized to be combined with a greenhouse, nutrition, temperature, water and light environment conditions which are needed by common head cabbage seedling growth are better provided, harmful influences on the seedling growing process caused by severe changes of environment conditions are avoided, and normal growth of the common head cabbage seedlings is guaranteed. By germinating seeds, cutting main root tips, managing and cultivating strong seedlings scientifically, the common head cabbage seedlings after seeding are short in main roots, multiple in fibrous roots, fast in survival after transplanting and field planting, good in growing trends and strong in disease resistance, and yield is improved by about 15%.

A root growth barrier and method for use with transplantable plants is provided. The barrier is a bilayer, comprising an inner layer of a root-tip-trapping material bonded to an outer layer of a root-impenetrable material. The inner layer faces the roots of a plant and serves to trap root tips to promote root branching. When a black or dark-colored inner layer is bonded to a white or light colored reflective outer layer, soil temperatures are lower and water retention is promoted. Such a barrier may be used as a freestanding container for plants or be manufactured into preformed rolls for use in plant pots and containers.

The invention relates to a Chinese yew aeroponic method. The Chinese yew aeroponic method comprises the steps of erecting a cultivation rack with a trapezoid cross section and laying planting plates on two side oblique planes of the cultivation rack; opening a spraying device and using electricity functional water and clear water to perform preliminary disinfection and cleaning on the inside and outside of the cultivation rack before field planting; selecting rapid propagating Chinese yew seedlings growing well, free of diseases and insect pests and provided with developed roots, arranging and trimming the root tip positions of root systems, planting the roots of the rapid propagating Chinese yew seedlings into planting holes of the cultivation rack, and using sponge blocks to plant Chinese yews into the planting holes; directly spraying a nutrient solution and water to the roots of the Chinese yews in an aeroponic mode to perform cultivation after field planting; shearing root hair after the root hair grow to meet enough length requirement. By means of the Chinese yew aeroponic method, the root hair is not planted in soil, is convenient to shear and can be repeatedly bred and sheared.

The invention relates to a gardening facility, in particular to a water-saving and fertility-maintaining type tree planting flower bed special for a roof. The tree planting flower bed consists of a flower bed main body, a planting cavity, a mud isolation pad, a stainless wire net, a water inlet valve, a drainage valve, an air bag, an air filled pipe, a sealing cover, a water storage cavity, a beam, a drainage pipe, an observation window and a water inlet pipe, wherein the internal space of the flower bed main body is divided into the upper planting cavity and the lower water storage cavity by an isolation belt which is formed by the beam, the stainless wire net and the mud isolation pad; the side wall of the water storage cavity is provided with the water inlet pipe, the drainage pipe and the air filled pipe; and the outer end of the air filled pipe is closed by the sealing cover, and the inner end of the air filled pipe is communicated with the strip-shaped air bag which is positioned in the water storage cavity. In the tree planting flower bed, the root of a tree can be watered from bottom to top, so water firstly reaches a water absorbing peak section deep in soil with the densest root tips, and the utilization ratio of the water is greatly improved; meanwhile, the tree planting flower bed has the advantages of fertility maintenance and is convenient to manage.

A wireless dental apex locator (10) for use in determining the location of the apex of a patient's root, includes an electronic module (13) having a battery power source (13a), an impedance analyzer circuit (13b) and a radio frequency transmitter (13c); a grounding module (12) having a clip (15) for grounding the patient; a probe module (14) having an endodontic probe (21); and an associated but not physically connected display unit (11) having a receiver (31) for receiving radio frequency signals from the transmitter (13c). The display unit (11) has an electronic circuit that conditions the signals and interprets the signal for display on a graphic display (31).

The invention provides a multicolor fluorescence in situ hybridization (MFISH) method for quickly analyzing and identifying alien chromosome of wheat. The method comprises the following steps: (1) preprocessing actively dividing tissues in meristematic zone of wheat root tip by N2O and conducting enzymolysis on the actively dividing tissues to obtain a metaphase specimen with clear images and uniform distribution of chromosomes; (2) marking a fluorescent probe of fundamental genome of wheat by nick translation method; (3) conducting fluorescence in situ hybridization on chromosomes at mitosis metaphase obtained in step (1) by using the constructed fluorescent probe in step (2); and (4) analyzing the fluorescence in situ hybridization results obtained in step (3). According to the method provided by the invention, not only can alien chromosomes and chromosome segments in distant hybrid wheat be identified, but also alien chromosomes and chromosome segments of distant hybrids of other crops can be identified.

The invention relates to a cultivation method of the triploid salvia, the steps of the invention are that: not completely ripe good salvia seeds are collected to put into the 1 / 2MS culture medium with colchicine for mutagenesis cultivation; then the seeds are transferred into a normal 1 / 2MS culture medium for cultivation, the root tip of the seedling is fetched to perform the ploidy identification of the chromosome after the preceding process of the chromosome, and the seedling with a chromosome quantity of 2n=4x=32 is the tetraploid salvia; the tetraploid salvia is taken as a female parent and the diploid salvia is taken as a male parent to perform the crossbreed and the obtaining seed is a triploid salvia plant. The nutrient organs such as the root, stem and leaf and so on of the triploid salvia plant obviously become bigger and present remarkable crossbreed advantages, and the root medicinal material output of the triploid salvia is increased by over 30 percent. Moreover, the triploid salvia has flowers, but no seeds, thus decreasing the nutrition consumption and the crossbreed advantages of the triploid salvia can be kept over 10 years through the asexual reproduction, which enhances the disease resistance and stress resistance with strong applicability, thus being applicable to planting in every part of the country. Besides, the crossbreed advantages produced by the novel variety triploid production are able to improve the output of the salvia medicinal material planting in a large scale.

A sheet forming method of paddy root tip, belongs to biotechnology domain. The method comprises: pretreating by ice water; dissociating by cellulase and pectinase; dyeing using phenol fuchsin; fixing using CarnoyI fixation fluid; sheet forming by pressed disk method. The sheet forming method uses cellulase and pectinase to dissociate and forms the sheet by pressed disk method, with virtues of conveniently operating and easily testing under microscope compared with the traditional smear method and with virtues of light background and dispersed chromosome compared with the traditional pressed disk method. The invention solves the defects of complicated operation of forming the sheet of the paddy root tip and difficulty of observing and counting the chromosome.

The invention discloses a multi-tooth-root implant for simulating a natural tooth root. The multi-tooth-root implant comprises an implant root part implanted into gum and a base part connected with the tooth crown, wherein the implant root part comprises a root neck and sub-roots; the sub-roots gradually contract from the root neck to the root tip; the contour line of each sub-root is a smooth curved line; the sub-roots are bent in the shape of a natural tooth root; the sub-roots are arranged in parallel; and the sub-roots are completely covered by the root neck in the overlook direction. The multi-tooth-root implant has the advantages of similarity to the natural tooth root and capability of excellently simulating the characteristics of biting and conductivity of the natural tooth.

The invention discloses a method for breeding meloidogynes by utilizing a single allosome of a separated root system of a non-exogenous cultured water spinach, and the method comprises the following steps: (1) preparing the separated root system of the water spinach: seeding the seeds of the water spinach onto a flat plate of a 1% water agar medium, cutting root tips when the seeds grow into rootsystem with the length of about 3-4cm, inoculating the root tips onto the flat plate of a non-exogenous separated root system culturing medium, and growing a large number of separated roots after 15-20 days; and (2) inoculating and breeding the meloidogynes: preparing oocysts of the meloidogynes into an egg grain suspension solution, inoculating the egg grain suspension solution onto the separated roots, obtained in the steps (1), of the water spinach, and culturing for 50-90 days, thereby obtaining a large number of signal meloidogynes. The method provided by the invention is high in breeding rate, simple in operation, difficult in pollution, wide in application scope and strong in practicability; the problem of difficulty in obtaining a large number of purified meloidogynes is solved; and the defects of the method for breeding the meloidogynes by utilizing potting seedling that time is wasted, a large space is occupied, the method is limited by natural and environmental conditions of seasons, the meloidogynes are easily polluted by other organisms, and the like, are avoided.

The invention discloses an improved method for detecting and identifying transgenic wheat with high sensitivity and high efficiency. The improved method comprises the steps of: (1) carrying out pretreatment and enzymolysis on tissue with vigorous cell division in a meristematic zone at a root tip of wheat through N2O gas to obtain a metaphase mitotic phase specimen with clear image and good dispersion of chromosomes; (2) marking a fluorescent probe of a target fragment to be detected (for example, a transgenic fragment carried by a transgenic carrier) by utilizing a nick translation method; (3) carrying out fluorescent in-situ hybridization on the metaphase mitotic phase chromosomes of the transgenic wheat obtained in the step (1) by utilizing the fluorescent probe of the target fragment of the transgenic carrier constructed in the step (2); and (4) analyzing fluorescent in-situ hybridization results obtained in the step (3), and comparing the fluorescent in-situ hybridization results with a contrast material to determine the distribution of fluorescent signals in the transgenic wheat to be detected. The improved method provided by the inventions can be applied to chromosomal localization of exogenous transgenic wheat with the sensitivity reaching 2-3kb, and also can be applied in the identification of transgenic signals of other crops.

The invention provides a method for inducing protocorms by utilizing dendrobe tissues. The method comprises the following steps: (1) carrying out routine asepsis on the obtained dendrobe tissues, wherein the dendrobe tissues are from the stem tip, root tip or tender stem section of dendrobe; and (2) carrying out induction culture on the dendrobe tissues subjected to asepsis in an induction culture medium firstly at the temperature of 23-25 DEG C and under a dark condition for one week and then at the temperature of 23-25 DEG C and under an illumination condition for 20-30 days with the illumination period of 12h / day, thus obtaining the protocorms. The method provided by the invention has the following main beneficial effects: (1) the method is simple in operation, low in production cost and less in environmental pollution; (2) the induction success rate is high, wherein the induction rate based on stem section culture is generally about 50%, the induction rate based on root tip culture can reach 60%-70%, and the best induction success rate based on the stem tip culture can reach about 75%; (3) the culture cycle is short, a large number of protocorms can be obtained within less time, and the induced protocorms can be directly subjected to aseptic culture so as to obtain a large number of seedlings; and (4) tissue culture is directly utilized, thus the characters of parents are well maintained.

The invention discloses a method for positioning immune tissues of growth hormone for malus plants and application thereof. The method for positioning immune tissues of growth hormone for malus plants comprises the following steps of: (1) fixing plant tissues to obtain fixed plant tissues; (2) carrying out slice making on the fixed plant tissues on the basis of the step (1) to obtain a plant tissue slice; and (3) immunostaining the obtained plant tissue slice on the basis of the step (2), and determining the distribution condition of hormone in the plant tissues through observing a position of a positive signal. The method for positioning immune tissues of growth hormone for malus plants can eliminate the easily caused influence of nonspecific staining of woody plant tissues, and can identify the positive signal clearly. The method for positioning immune tissues of growth hormone for malus plants verifies that the growth hormone has tissue specificity when being distributed in the tissues of a stem tip or a root tip of an apple tree in a condition of iron deficiency.

The present invention relates to an artificial inductive flowering asexual breeding method of bulbil konjak. Said method includes the following steps: making the bulbil konjak bulb undergo the process of flowering induction, inhibiting germination of lateral bud on the bulb and making the root tip grown out on the bulb be perished, promoting differentiation of top bud of konjak bulb to form flower bud, after the bulb is transplanted, artificially inhibiting flowered leafing plant growth, making flower bract can be matured and seeded so as to obtain lots of seeds capable of reproducing konjak.

The invention discloses a genetic particle swarm multi-disciplinary design optimization algorithm-based guided missile parameter design method, relates to a guided missile parameter design method, and aims at solving the problem that the conventional intelligent optimization algorithm-based guided missile parameter design method is not suitable for the conditions of more multi-disciplinary design parameters and variable coupling phenomenon. The method comprises the following steps: selecting an engine throat diameter, an engine combustion surface area, an engine charge thickness, a guided missile outer diameter, an engine specific impulse, engine mass, fuel mass, a missile wing root chord length, an empennage root tip length and an empennage tip chord length as design parameters; and optimizing three control parameters w, c1 and c2 and design parameters in a standard particle swarm algorithm by utilizing a genetic algorithm, and introducing a genetic thought into a particle swarm position updating process when optimizing the particle swarm algorithm in the optimization process. The method disclosed in the invention is suitable for the field of guided missile parameter design.

The invention discloses a rapid breeding method of monoploid radish and belongs to the field of plant genetics and breeding. The method comprises the following steps: culturing radish microspores that are subjected to heat treatment at 32.5 DEG C in a constant-temperature incubator for 48 hours to obtain cotyledonous embryoids, culturing the cotyledonous embryoids for 5-7 days at 25 DEG C under 4000Lx light illumination (16 h / d), transferring to a subculture medium for germination until the embryoids turn green, transferring to a rooting medium for plant regeneration, and identifying the regenerated plants by root tip cell chromosome count method or flow cytometry. The method is successful in the culture of regenerated plant of monoploid radish and increases the induction rate of embryoid from microspore up to 17.03 / 105. The self-bred line of monoploid plant of radish cultured by microspore culture method is obtained by chromosome doubling and is used for genetic improvement and new variety breeding of radish.

The invention provides a grafting method for hybrid citrus seed seedlings. The method comprises the following steps: (1) collecting seeds, namely starting to collect the seeds when fruits grow for 150 days, wherein the harvesting period of the seeds starts from flowering pollination; (2) breeding the seedlings, namely starting from radicle germination after the seeds are sown, breeding hypocotyledonary axes which vertically grow for 6-7 days until the length of the hypocotyledonary axis exceeds 2cm, cutting root tip growth points, keeping remaining radicles, mesocotyl thereon, cotyledon and germ parts, and breeding for 1-2 days for grafting; (3) performing grafting, namely cutting off the top ends of stock container seedlings which are one year old over the ground for 40cm, grafting hybrid seedlings at the cutting openings of the top ends of the stock container seedlings by using a cut grafting method, and sleeving a plastic film bag. According to the grafting method for the hybrid citrus seed seedlings, the seedling survival percent of the hybrid citrus seed seedlings is improved to be greater than or equal to 99 percent, the grafting survival rate is improved to be greater than or equal to 95.9 percent, and the growth of grafting buds is accelerated.

The invention discloses a preparation method of a sea cucumber epiphytic Bacillus subtilis exopolysaccharide. The method has the characteristics of simple operation and low cost, and can stably acquire the exopolysaccharide of sea cucumber epiphytic Bacillus subtilis. At the same time, the sea cucumber epiphytic Bacillus subtilis exopolysaccharide prepared by the method has strong antioxidant activity, can inhibit the formation of Vicia faba root tip cell micronucleuses, and can be widely used in the anti-tumor medicines, biological control, food preservation, corrosion prevention, cosmetic additives and other fields, thus having broad market prospects and industrial value.

The present invention relates to a method for filtering crop stress-resistant strain and relates to a method for filtering crop strain. The method solves the problems of the existing method for filtering and cultivating the stress-resistant strain of long filtering period, high cost, complex operation and various uncertain factors. In the method, seeds are dipped in aqueous solution which uses NaCl or PEG as a solute and has different mass concentrations; the seeds germinate generally; then the solution concentration with the rate of emergency being 4 percent to 6 percent is selected as the concentration for filtering the stress-resistant strain; a seed young seedling is prepared firstly; the seed young seedling is dipped in the aqueous solution which uses NaCl or PEG as the solute and has different mass concentrations for stress treatment and cleaned; then the seed young seedling is dipped in distilled water to be cultivated; and the solution mass concentration with the seed young seedling root tip regenerating rate being 4 percent to 6 percent is selected as the concentration for filtering the stress-resistant strain. The height and the stem diameter of a plant obtained by the method are larger than the height and the stem diameter of unfiltered plants, and the method reduces the growth period greatly, has simple operation and reliable data and reduces the planting cost by 20 percent to 50 percent.

The invention discloses a dendrobium officinale rapid breeding method through root tip tissue culture. The breeding method comprises the following steps: subjecting the root tip to induced differentiation, culturing the root tip, performing proliferation culture, carrying out rooting culture to obtain rooted seedlings, and finally performing acclimatization and transplanting to obtain finished seedlings. The cultured dendrobium officinale seedlings inherit the excellent characters of artificially-selected plants, the resistance is good, the yield is stable, and thus the seedlings are worthy to promote.