The invention discloses a preparation method and application of
canna edulis stem cells, and belongs to the technical field of stem cells. The
canna edulis stem cells are prepared by the following steps: A1, selecting
canna edulis which is large in tuber, many in root, slightly red in
bud end, good in growth vigor, free of breakage, germs and
insect pests, cleaning, sterilizing,
cutting a
root tip meristematic region of the canna edulis into blocks, continuously sterilizing, infiltrating by using a
sucrose solution, and washing by using an
antioxidant solution; a2, inoculating the small blocks treated in the step A1 into a first culture medium for induction culture; a3, taking a
cell population containing stem cells after induction culture in the step A2, and inoculating the
cell population into a second culture medium for multiplication culture; and A4, inoculating the stem cells subjected to multiplication culture in the step 2 into a third culture medium for adaptive culture, and inoculating the stem cells into a fourth culture medium for
suspension culture to obtain the canna edulis stem cells. The canna edulis stem cells can be applied to
crop planting, and the
crop yield can be obviously increased; the canna edulis stem cells can also be applied to health care products and
cosmetics with anti-aging effects.