19 results about "Root tip" patented technology

The invention discloses a plant growth-promoting bacterium and application thereof, relates to the field of microorganisms, and provides a plant growth-promoting bacterium which can generate a growth-promoting effect on corn plants and further improve the growth condition of the corn plants. The growth-promoting bacterium is pumilus minutus E2 and is preserved in the China Center for Type Culture Collection, the preservation address is Wuhan University in Wuhan, the preservation date is September 19, 2025, and the preservation number is CCTCC NO: M 20252071. By applying the strain to a corn culture medium, the bifurcation number, the root tip number, the root length, the dry weight, the fresh weight and the plant height of corn can be increased, the growth promoting effect of the strain on corn plants is proved, and a basic support is provided for further application to production.

The application discloses a kind of intelligent preparation systems of laser navigation milk teeth root canal, it is related to milk teeth root canal treatment field, including: image processing module, self-checking registration module, structure acquisition module, spectral processing module, anomaly detection module, fusion determination module, compensation tracking module, digital modeling module, simulation learning module, trajectory generation module, execution monitoring module, safety protection module, interactive command module and record audit module;The application can more accurately distinguish infected dentin and healthy dentin, reduce the risk of misjudgment or missing infected areas, effectively reduce the risk of root tip penetration and root tip tissue damage, so that the laser cutting process always revolves around the real infected area, both can fully remove infected tissue, and can maximize the retention of healthy dental structure, improve root canal preparation efficiency and safety, reduce the dependence of doctors on experience judgment, so that milk teeth root canal preparation operation is more standardized and repeatable.

The invention discloses application and a preparation method of an alfalfa tissue culture hairy root tip chromosome slice, and the preparation method comprises the following steps: (1) preparing an explant by using mature alfalfa leaves, and then mediating hairy roots to transform the explant by using agrobacterium rhizogenes to obtain hairy roots; and (2) carrying out chromosome slide preparation by using the hairy roots obtained in the step (1). According to the method disclosed by the invention, the hairy roots cultured in vitro are used as chromosome slide preparation materials, and the hairy roots cultured in vitro are generated by induction of agrobacterium rhizogenes, have the characteristics of hormone autotrophy and infinite growth, are rapid in growth, stable in heredity and easy to operate, can be subcultured and preserved for a long time under an aseptic condition, realize material taking as required, are not limited by seasons and growth cycles, and are suitable for large-scale production. And the flexibility and repeatability of the experiment are greatly improved.

The application discloses a pile channel preparation guide device and a manufacturing method thereof, and belongs to the technical field of oral cavity restoration. The application aims to manufacture a pile channel preparation guide device which can guide a doctor to accurately and simply realize the direction preparation and depth control of a predetermined pile channel. The pile channel preparation guide device manufactured by the manufacturing method has a retainer which can be embedded in a target dental pulp chamber. A guide structure is arranged in the retainer, and the guide structure has a direction which is extremely suitable for the preparation of a target root channel. The guide structure can effectively ensure the accuracy of the direction of the pile channel preparation. The length of the guide structure is equal to the working length recorded during the preparation of the root channel, minus the length of the target root channel from the target root channel opening to the root tip of the target root channel, and plus the reserved root tip sealing length. Therefore, the guide structure has a length which is extremely suitable for the preparation of the target root channel. It can be seen that the doctor can accurately and simply realize the preparation of the direction of the predetermined pile channel and the control of the depth by using the pile channel preparation guide device manufactured by the manufacturing method.

The invention discloses a golden papaya tissue culture seedling culture medium block as well as a preparation tool and a preparation method thereof. The golden papaya tissue culture seedling culture medium block comprises a first base block and a second base block, the first base block is an agar base block, and the first base block is of a cylindrical structure; a filling material of the second base block is formed by mixing coco coir, perlite and vermiculite according to the volume ratio of 3: 1: 1, the filling material is subjected to high-pressure sterilization treatment, and the water content of the filling material is adjusted to 60%-70%; the second base block is of a cylindrical structure, a first base block containing groove is formed in the upper portion of the second base block, and the first base block can be inserted into the first base block containing groove; and the filling material of the second base block contains a root growth promoting agent. According to the culture medium block, the tedious operation of seedling taking, agar washing and transplanting in a traditional process is avoided, mechanical damage such as root tip breaking, root epidermis tearing and lateral root falling is fundamentally eliminated, and the tissue culture seedling transplanting survival rate can be remarkably increased.

The invention relates to the technical field of medical instruments, in particular to dental pulp nerve cell filling paste and a preparation method thereof, and the filling paste comprises the following components: 8-12% of an inorganic filler, 12-18% of an antibacterial filler, 5-8% of slow-release antibiotic microspheres, 8-12% of an nHA-Col collagen composite material, 1-3% of a traditional Chinese medicine extract, and the balance of a liquid agent. According to the technical scheme, growth and reproduction of pathogenic microorganisms can be effectively inhibited, two schemes of protecting dental pulp nerve cells, opening pulp and not opening pulp are achieved, and the problems of multiple complications such as over-changeable blackening and breaking of the existing nerve-killing and root-tip-extraction nerve excision tooth tissue surface are successfully solved; the purpose that the permanent dental pulp nerve cell filling can be completed by one-time minimally invasive rust removal of a patient in about 15 minutes is achieved, the dental pulp nerve cells are protected, dental pulp nerves are not killed, apical nerve cells are completely filled at one time, and time and cost are saved.

1. Name of the product in this design: Root Tip Electronic Analyzer. 2. Purpose of this design: A tool for measuring root canal length during dental treatment. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

This invention belongs to the field of fruit tree cultivation technology, specifically relating to the application of methyl jasmonate in improving nitrogen use efficiency in plants. Applying a certain concentration of methyl jasmonate can significantly improve the nitrogen use efficiency (NUE) of plants under limited nitrogen supply, specifically manifested in a significant increase in plant dry and fresh weight, total root length, total surface area, total volume, number of root tips, number of lateral roots, and root activity; an increase in plant photosynthetic capacity and nitrogen content, and a decrease in the carbon-to-nitrogen ratio. This indicates that an appropriate concentration of methyl jasmonate effectively improves the NUE of apple plants under limited nitrogen supply, promoting plant growth and laying the foundation for further research into the functional mechanism of methyl jasmonate in improving apple NUE. Furthermore, these findings may be applied in the future to guide production practices and improve the nutritional status of fruit trees.

The utility model discloses a multi-purpose accurate suck-back device for a microscopic root tip operation. The multi-purpose accurate suck-back device comprises a suction head, a suction handle, an access pipe, a connector, a metal sheet and a magnetic suction fixing device. A metal sheet is arranged in the suction head and can be bent in the direction of the tooth root, the suction head accurately enters a root canal of an inverted prepared diseased tooth, and scraps and liquid in an operation area are removed by combining negative pressure suction and positive pressure liquid. And the buckle is connected with the upper end of the handle. The design of the suction handle is suitable for pen-holding type grasping, and the adjustable spring interface of the lower end connector is suitable for most modern dental chairs. A magnetic attraction fixing device and a spring clip further improve the fixity of the device. The device has multiple effects, is practical, accurate to operate, convenient to use and low in cost, overcomes the defect that root tip liquid and chippings are retained due to the fact that a traditional root canal aspirator cannot penetrate into a root canal of an inverted preparation affected tooth, and is suitable for cleaning an operation area before inverted filling of a microscopic root tip operation.

This invention relates to the field of plant genetic engineering, specifically to the MMR1 protein, its encoding gene, and its applications in regulating ROS homeostasis in the root tip meristem of plants. This invention involves knocking out the MMR1 gene to obtain stable transgenic lines of Arabidopsis thaliana. mmr1-1, mmr1-2 and build MMR1 Gene replacement lines and MMRI Gene overexpression lines; mutants compared to wild type mmr1-1, mmr1-2 The results showed shortened root length, shortened root tip meristem, and reduced root tip division ability; reintroduced lines indicated that the MMR1 protein was located in the mitochondria; the roots of the overexpressing lines were longer than those of the wild type, indicating... MMR1 The gene plays an important role in root elongation, root apical meristem development, and root apical meristem cell activity in Arabidopsis thaliana. This invention fills a gap in the field of mitochondrial-localized protein regulation of root development and ROS homeostasis, providing new genetic resources for root improvement and transgenic breeding.

The invention discloses a detection method for rapidly and visually judging the damage resistance of potatoes based on root tip auxin distribution. The detection method comprises the following steps: sample preparation and sensor loading: controlling expression of an auxin biosensor AuxSen in a potato root tip sample by adopting an inducible expression system; performing reference auxin distribution imaging and standardized injury stimulation: performing multispectral imaging on a sample root tip to obtain reference FRET data, and synchronously acquiring environmental parameters; performing real-time auxin dynamic monitoring and feature extraction: performing time sequence FRET imaging on the damaged root tip, automatically identifying the response starting time of the auxin by adopting a change point detection algorithm, and analyzing and quantifying the flow rate and direction of the auxin through a vector field; the change point detection algorithm performs unsupervised automatic judgment on response starting time by statistically analyzing time points where significant mutation occurs in FRET time sequence data; and anti-damage capability judgment: outputting quantitative indexes of the anti-damage capability of the potatoes.

To provide a method for manufacturing an implant having a completely different shape from conventional methods, in which the shape of the implant is adapted to each site where it is to be embedded. [Solution] This is a method for manufacturing an implant (100) used in place of a maxillary molar, which is formed by integrating three separate root equivalent portions (121, 122, 123) with one crown prosthesis portion (150) connected to the base end of the root equivalent portions, and has an abutment (170) attached. One or two vertical grooves (135) having a predetermined depth are formed on the inner surface of each root equivalent portion, extending from the tip of the root toward the base end of the root over approximately one-third of the length of each root equivalent portion, and horizontal grooves (111, 112, 131) of a predetermined depth are formed in a row at predetermined intervals on the surface area of ​​each root equivalent portion, from the underside of the cervical equivalent area formed on the abutment attachment side of the crown prosthesis toward the root tip of each root equivalent portion.

This invention discloses a novel indole compound with the following chemical structural formula: This invention utilizes fungal co-culture fermentation to obtain a new natural compound that exhibits good inhibitory activity on the growth of young roots of barnyard grass and has a significant damaging effect on its root tip cells.

The invention relates to the field of plant tissue culture, in particular to a method for tissue culture and propagation of phalaenopsis by taking a root tip as an explant. In particular, the method involves a step of using an adventitious bud induction medium, an adventitious bud proliferation medium and / or a rooting medium; the method is suitable for a plurality of varieties, and has important significance for realizing annual and industrial production of phalaenopsis and reducing the production cost.

The present application relates to the technical field of plant tissue protoplast separation, and in particular to a preparation method and application of Phyllostachys edulis root tip protoplast. The preparation method comprises the following steps: collecting root tips of Phyllostachys edulis spring bamboo shoots as raw materials; washing the raw materials with 0.6-0.8M mannitol, and then placing the washed raw materials in an enzymatic solution for enzymolysis for 2.5-3.5h to obtain a protoplast single cell suspension; centrifuging the protoplast single cell suspension at 40g-60g, removing the supernatant, resuspending the cells with mannitol, and obtaining a cell resuspension; mixing the cell resuspension with a sucrose solution with a mass concentration of 18-22%, and then centrifuging at 40g-60g to obtain the protoplast. The preparation method can obtain protoplast with high cell activity and pure background, and can meet the use requirements of Phyllostachys edulis root single cell transcriptome sequencing library construction, and has great significance for Phyllostachys edulis root single cell sequencing.

This invention discloses a strain of *Bacillus hygroscopicus* with functions of phosphorus solubilization, promoting plant growth, and enhancing plant drought resistance, and its applications. The *Bacillus hygroscopicus* is *Bacillus hygroscopicus*. Bacillus height Strain B27, with its registration number CGMCC No. 36877 at the China General Microbiological Culture Collection Center, has been shown to exhibit significant improvements in several experiments. In tobacco inoculation experiments, compared to the control group, the B27-inoculated tobacco seedlings showed a significant increase in lateral root number, root hair length, and aboveground and belowground fresh weight. Similarly, in apple rootstock inoculation experiments, compared to the control group, the B27-inoculated apple plants showed significant increases in plant height, aboveground fresh weight, root surface area, root tip number, root hair length, root hair quantity, and phosphorus content. Finally, in Arabidopsis thaliana inoculation experiments, compared to the control group, the B27-inoculated Arabidopsis seedlings showed a significant improvement in drought resistance.

The invention discloses a garlic root tip culture and polyploidy induction integrated device, and relates to the technical field of garlic breeding, the garlic root tip culture and polyploidy induction integrated device comprises a tank body and a control device, a culture disc is arranged in the tank body, a plurality of uniformly distributed culture grooves are formed in the culture disc in a penetrating manner, and a driving mechanism for driving the culture disc to rotate is arranged on the tank body; a bearing mechanism is arranged in the tank body, and a feeding mechanism used for feeding for cultivation and induction of garlic root tips is arranged in the tank body. According to the device, atomized inducer liquid drops can be sprayed out in different directions when being sprayed, so that the atomized inducer liquid drops can be quickly and uniformly dispersed, and garlic is driven to rotate around the axis of the tank body in cooperation with the culture disc; therefore, the atomized inducer liquid drops can be uniformly attached to the surface of the root tip of the garlic, and the induction effect on the root tip of the garlic is further guaranteed.

The present application relates to the technical field of plant chromosome preparation, and specifically discloses a method for increasing the number of mitotic metaphase chromosomes of sugarcane or sugarcane relative genus root tips, comprising: soaking sugarcane or sugarcane relative genus stem double bud segments in an indole-3-acetic acid solution with a concentration of 60-120 mg / L, and culturing in sand or peat substrate; root tip pretreatment: culturing for 2-3 days, selecting healthy and tender root tips, and placing the root tips in saturated p-dichlorobenzene aqueous solution for low-temperature treatment at 8-12 DEG C for 3-5 h; using Carnoy's fixative for fixation; enzymolysis of the root tip growth point; smearing the root tip on a glass slide for preparation; and staining treatment. The method for preparing the present application can achieve the fastest time of only 4 days from root tip culture to chromosome observation, has a short cycle, high efficiency, is convenient and fast for sampling, has a large number of metaphase division phases in the chromosomes, clear morphology, a clean glass slide background, and is convenient for chromosome counting observation and karyotype analysis, and has important significance for the development of sugarcane cytogenetics research.