166 results about "Gerbera" patented technology

The invention provides a high-efficiency propagation African daisy-producing method by utilizing in vitro blades, which comprises the selection of an explant, the establishment of an aseptic explant system, the induction of adventitious bud and secondary multiplication culture, particularly comprises the induction culture of the in vitro blades, the secondary multiplication culture of the adventitious bud on the in vitro blades, and the rootage culture of the adventitious bud. The method greatly improves reproduction and production efficiency, shortens seedling production period, and accelerates the application of new species; by statistics, the inductivity and the reproduction rate of the blades after being cultured for 1 month are respectively 85.4 to 85.9 percent and 34.9 times; the method solves the problems of the existing African daisy in the tissue culture rapid propagation production that the propagation speed is low and the seedling quality is poor, etc., the propagation efficiency and the production efficiency of the tissue culture rapid propagation can be improved within short period, the propagation and rootage efficiencies of 50 days are respectively 8.4 to 10.8 times and 129 and 100 strain per block; furthermore, the aseptic blade does not need disinfection, has more material, can be carried out in any stage of the tissue culture rapid propagation, and has actual application value to self-culture and the generalization of new species, the rapid propagation of detoxication original seed, and the rejuvenation of production species.

The invention discloses an efficient propagation method for African daisy virus-free seedlings, which comprises the following steps: (1) carrying out pretreatment on African daisy virus-free seedlings; (2) inducing adventitious buds; (3) cutting the adventitious buds, and carrying out subculture multiplication; (4) hardening the seedlings; (5) carrying out water culture for rooting; (6) carrying out water culture for reinforcing the seedlings; and (7) transplanting. The efficient propagation method disclosed by the invention solves the problems of low efficiency and easy secondary pollution of the African daisy virus-free seedlings in a propagation process, is capable of propagating a lot of good African daisy virus-free seedlings in a short time and also avoiding the problem of variety degeneration caused by African daisy division propagation and seed propagation, is simple to operate and easy to realize industrialized popularization, and has relatively high real values in production.

The invention relates to a shelled shrimp chili sauce and a preparation method thereof. The shelled shrimp chili sauce is characterized by being prepared from the raw materials in parts by weight: 3 to 4 parts of fortune's drynaria rhizome, 4 to 5 parts of Chinese angelica, 2 to 3 parts of Japanese thistle herb or root, 3.5 to 4 parts of rhizome of large-headed atractylodes, 4 to 5 parts of leonurus heterophyllus, 1 to 2 parts of bitter herbs, 2 to 3 parts of gerbera, 30 to 34 parts of shelled shrimps, 300 to 350 parts of broad bean paste, 2 to 3 parts of olive oil, 12 to 14 parts of peanuts, 15 to 18 parts of chickpeas, 50 to 55 parts of red peppers, 3 to 4 parts of honey, 10 to 12 parts of white vinegar, 3 to 4 parts of toadstool, 2 to 3 parts of fragrant mushrooms, 32 to 35 parts of glutinous rice, and 7 to 8 parts of nutrition additives. The shelled shrimp chili sauce is spicy and refreshing, and appetite can be whetted; at the same time, the shelled shrimp chili sauce is rich in nutrition, and the added shelled shrimps contain rich proteins, vitamins A and minerals of potassium, iodine, magnesium, phosphorus and the like, so that bodies can be benefitted; in addition, the shelled shrimp chili sauce further contains various Chinese herbal medicine components, so that if users often eat the shelled shrimp chili sauce, the efficacies of tonifying kidneys and strengthening bones, replenishing blood and activating blood circulation, harmonizing spleens and stomachs and benefiting stomachs, and clearing heat and removing toxicity can be achieved.

The invention relates to an excised high frequency regeneration propagation method of an African chrysanthemum. In the invention, African chrysanthemum test-tube plantlet is the material of high frequency excised regeneration. The method comprises the culture steps of receptacle initially generation culture, callus induction, adventitious bud differentiation and subculture multiplication, rhizogenesis culture and test-tube plantlet transplantation and so on. Optical culture medium is prepared at each stage of culture and propagation which consists of receptacle initially generation culture medium, callus induction initially generation induction culture medium, callus induction culture medium, callus multiplication and differentiation culture medium, rhizogenesis culture medium and transplantation culture medium. The invention provides suitable temperature and illumination conditions, through which, the African chrysanthemum can propagate rapidly with the excised high frequency based on petiole to manufacture germchit. The invention can be applied in the genetic transformation of the African chrysanthemum, meeting the requirements of African chrysanthemum germchit market and breeding genetic engineering.

The invention discloses a method for identifying gray mold resistance of gerbera, belonging to the technical field of plant disease resistance identification. The method comprises the following steps: (1) selection of proper inoculation material: selecting freshly collected and integrated young leaves and petals as the inoculation material; (2) culture of inoculation material and preparation of conidia suspension; (3) preparation of inoculation liquid: before inoculation, diluting the conidia stock solution into an inoculation liquid containing 3 wt% of potato glucose and 1*10<6> / ml conidia, or into an inoculation liquid containing 6wt% of potato glucose and 3*10<5> / ml conidia; and (4) inoculation. The method disclosed by the invention is carried out under manually controlled stable conditions, and achieves the goal of identifying the gray mold resistance of gerbera in a simple, quick and accurate way; and the accuracy of the identification result is high.

The invention discloses an ex-vitro soilless cutting rooting method for tissue-cultured and proliferated seedlings of gerbera jamesonii bolus. The method mainly comprises the following steps of: transferring induction-cultured buds to a proliferation culture medium to culture, wherein the proliferation culture medium is MS (Murashige and Skoog) basic culture solution containing 0.3-0.7 mg / L of 6-BA (6-benzylaminopurine), 0.1 mg / L of NAA (naphthylacetic acid), 30000 mg / L of cane sugar, and 6500 mg / L of agar; when the proliferated seedlings are grown, moving a proliferation culture bottle in a greenhouse covered by a sunshade net having a light-shading rate of 70% to perform seedling hardening for 4-7 days; and then performing ex-vitro soilless cutting rooting culture on the proliferated seedlings. According to the invention, a rooting phase is soilless rooting culture in the greenhouse, thus enhancing the environmental adaptability of the rooted seedlings, ensuring the rooting rate of the seedlings, increasing the survival rate of cutting transplantation, keeping the technical advantages of tissue culture, overcoming the problems of low seedling-hardening survival rate, high production cost, many production links and the like of the tissue-cultured seedlings, and reducing soil-borne diseases at seedling stage; therefore, high-quality seedlings can be provided for soilless culture for gerbera jamesonii bolus.

The present invention relates generally to a genetic sequence encoding a polypeptide having flavonoid 3′,5′-hydroxylase (F3′5′H) activity and to the use of the genetic sequence and / or its corresponding polypeptide thereof inter alia to manipulate color in flowers or parts thereof or in other plant tissue. More particularly, the F3′5′H has the ability to modulate dihydrokaempferol (DHK) metabolism as well as the metabolism of other substrates such as dihydroquercetin (DHQ), naringenin and eriodictyol. Even more particularly, the present invention provides a genetic sequence encoding a polypeptide having F3′5′H activity when expressed in rose or gerbera or botanically related plants. The instant invention further relates to antisense and sense molecules or RNAi-inducing molecules corresponding to all or part of the subject genetic sequence or a transcript thereof. The present invention further relates to promoters which operate efficiently in plants such as rose, gerbera or botanically related plants.

The present invention discloses the fast germchit propagating method for flameray gerbera cut flower, and aims at providing one kind of fast propagating method of flameray gerbera germchit with less germchit variability, consistent characteristic and robust growth. The method includes cutting the floral receptacle of flameray gerbera bud as the explant, cutting the floral receptacle into small blocks, culturing in inducing culture medium at proper lighting and temperature to grow indefinite bud, culturing the indefinite bud in proliferating culture medium at proper lighting and temperature to grow bush bud, cutting the bush bud into single seedlings, culturing the seedlings in rooting culture medium at proper lighting and temperature to induce rooting, and finally transplanting the rooted seedling in seedling bed. The present invention is applied in propagation of flower.

The invention discloses a gerbera extracting solution as well as a preparation method and application thereof. The gerbera extracting solution prepared in a water extraction manner has the effects of inhibiting and removing a series of reactive oxygen species in the cell metabolism process, so as to prevent free radical-mediated oxidative damage and stabilize cytomembrane. The gerbera extracting solution has hydratation of resisting oxidation and maintaining cutin cell stability, and has the effect of preventing loss of a natural moisturizing factor by the characteristics, so as to delay skin senescence, and the gerbera extracting solution has significant moisturizing and anti-aging effects when being applied to cosmetics.

A flower cup having a flared bell-like shape has a flared rim, tapered sides and a substantially flat base. The rim, sides and a portion of the base have a plurality of perforations running from the rim to the base, where the perforations meet a plurality of lines which radiate from the center of the base. The plurality of side perforations can be six and the plurality of base lines six. The side perforations align with the base lines. The plurality of base lines are partly perforated and partly cut, or half of the base lines are fully cut and the other half of the base lines are partly cut and partly perforated. The flower cup can have a stippled finish. A flower-stem protector has a straw sized to accommodate flower stems and having at least one rib on its proximal end, and a collar affixed to the proximal end of the straw.

The invention provides wine cubes and belongs to the field of food processing. The wine cubes are formed mainly by sticky rice, oriental stephania roots, lalang grass rhizomes, areca nuts, herbs of pilose gerbera, herbs of ceylon leucas, ardisia crenata sims, Illicium simonsii, crneate lespedeza, licorice, radix asparagi, chrysanthemums, ginseng, the piper wallichii, longhairy antenoron herbs, Christina loosestrife, kudzu vines, licorice roots, paris polyphylla and red wheat. The invention further discloses a preparation method of the wine cubes. The piper wallichii has the good effects of strengthening yang and tonifying yin, dispelling wind and removing obstruction in the meridians, expelling wind-damp and treating waist-leg weakness, then the oriental stephania roots, the lalang grass rhizomes, the areca nuts, the herbs of pilose gerbera, the herbs of ceylon leucas, the ardisia crenata sims, the Illicium simonsii, the crneate lespedeza, the licorice, the radix asparagi, the chrysanthemums, the ginseng, the piper wallichii, the longhairy antenoron herbs, the Christina loosestrife, the kudzu vines, the licorice roots, the paris polyphylla and the red wheat are added, and in this way, the prepared wine cubes are rich in nutrient and free of toxic and side effects by the adoption of the pure natural raw materials.

The invention discloses a paddy salt tolerant protein OsSRM. The protein has an ammonia acid sequence shown in the sequence list SEQ ID NO.1. The protein which has excessive expression in paddy seedlings can improve the high salt and high temperature tolerance of the paddy seedlings. If the gene coding protein is converted into the plants of vegetables and flowers such as Arabidopsis thaliana, capsicum, eggplant, strawberry, scarlet sage, flameray gerbera, and the like, the protein can improve the high salt and high temperature tolerance of the plants, help increase the yield of paddy, open field vegetables and flowers in the alkaline land, and improve the utilization of the alkaline land in the coastal areas of the country. The protein can overcome the continuous cropping barriers caused by soil salt return or high temperature under the current facility conditions, improve the yield and quality of the plants of vegetables and flowers, and promote agricultural efficiency improvement and farmer income increase.

The invention discloses a food retention-removing and eyesight-improving type refreshing chicken cutlet, which is prepared by the following raw materials in weight part: 55-60 parts of chicken breast, 8-12 parts of chestnut kernels, 7-8 parts of sesame, 12-16 parts of coriander crumbles, 0.3-1 part of green tangerine peel, 1-2.3 parts of gerbera, and 1.2-2.2 parts of pearl powders. Appropriate amounts of clove oil, thyme oil, coriander seed oil, sucrose ester, chitosan, 1-2% of acetum, sunflower seed oil, ground pepper, white sugar, five spice powders and soybean sauce are added. The obtained chicken cutlet is savoury, mellow, delicious and good in mouth feel. The chicken cutlet is coated by a chitosan-compounded preservative film preparation. Compared with a single chitosan-based film solution, the chitosan-compounded preservative film preparation is better in fresh-keeping effect, and contents coated by the chitosan-compounded preservative film preparation are slowed down in hydrogen sulfide generation, lipid oxidation and bacteria multiplication. The chicken cutlet can be preserved longer by 5-7 days. Meanwhile, through adding green tangerine peel, gerbera, pearl powders and other materials, the refreshing chicken cutlet has the health-care functions of soothing the liver, relieving the stagnant Qi, removing the food retention, transforming the stagnation, soothing nerves and improving the eyesight.

The invention discloses a flax liver calming bolete sundry fungus soup base and a preparation method thereof. The flax liver calming bolete sundry fungus soup base is characterized by being prepared by the following raw materials including, by weight, flax, platycladi seeds, oroxylum indicum, hematoxylon, calamus, solanum lyratum thumb roots, roots of privet-like premna, gerbera, bolete, black fungus, grifron maitake, garlic, green onion, fresh ginger, cassava starch, salt, oat powder and anise; the soup base is prepared by the working procedures including cleaning, raw material processing, mixing, decontamination and sterilization bagging. The flax liver calming bolete sundry fungus soup base has the advantages that the taste is delicious, the storage is convenient, the liver calming effect is achieved, in addition, the preparation technology is more simple and hygeian.

The invention discloses a planting method of African daisy. The method comprises the following steps that 30-40 cm soil deep ploughing is performed, plant ash, humus, calcium superphosphate and compound fertilizer are applied into soil and then 15-25 cm deep ploughing is performed; a 70-100 cm deep drainage ditch is performed nearby, and the width of the ditch is 40-45 cm. Due to the fact that the roots of African daisy are developed, a transplanting bed at least needs 30 cm or above deep soil layer; accordingly, deep furrow ridge can be formed, the height is 45-50 cm, and the width is 60-65 cm; a garlic emulsion and phytobiotics are uniformly sprayed to soil, the ridge is covered with a plastic film rapidly, sealed and smoked after spraying is performed, the film is removed after 2-3 days, the soil acidity and alkalinity is controlled to be 6-6.5 after the film is removed, and field planting can be performed.

The invention relates to a method for transgenically obtaining a nosomycosis resistant cut gerbera flower. The method obtains a transgenic plant through the following steps of: separating an antifungal gene, namely a chitinase gene Chi and a beta-1, 3-dextranase gene Glu, cloning the Chi and the Glu, constructing a bivalent expression vector, constructing a high-efficient genetic transformation regeneration system of the cut gerbera flower, transformation of the cut gerbera flower by the antifungal gene and so on. The method uses CaMV35S as starter and transforms genes like Chi, Glu, Npt II and so on into a cut gerbera flower variety 'redcap' and then obtains the transgenic plant, thereby providing technical support for obtaining a large number of antifungal transgenic African daisy strains. The method effectively provides the antifungal transgenic African daisy strains and simultaneously provides a quick, effective and stable technical path for African daisy genetic engineering breeding.

The invention belongs to the technical field of pesticides and particularly relates to a biological pesticide for vegetables and a preparation method thereof. The active ingredients of the biological pesticide for vegetables are prepared from, by weight, 40-60 parts of purslane, 25-45 parts of rhizome of Japanese fleeceflower, 30-40 parts of tobacco leaf, 28-56 parts of rhizome of tatarinowii sweetflag, 15-35 parts of alum, 20-30 parts of root of devalay gerbera, 30-40 parts of root of common peony, 10-20 parts of heartleaf houttuynia, 11-22 parts of belvedere fruit, 22-40 parts of sweet wormwort, 18-36 parts of root of common threewingnut, 15-35 parts of root of Chinese pulsatilla, 10-20 parts of fruit of Chinese hawthorn, 10-20 parts of clove, and 3-6 parts of a suspending agent. The biological pesticide for vegetables has the advantages of good effect and zero toxic and side effects, has significant pest-expelling, fungicidal and pesticidal effects, can prevent disease and pest damage during vegetable growth and prevent pesticide resistance, can even promote the growth of vegetables and the absorption of nutrients in soil, and may act as an ideal environment-friendly agricultural vegetable protectant.

The invention discloses a plant extraction composition with an anti-aging effect. The composition comprises the following raw extracts in parts by weight: 20-30 parts of rehmannia, 35-45 parts of scandent schefflera stem and leaf, 30-40 parts of Talinum crassifolium and 25-35 parts of gerbera. Compared with the prior art, the plant extraction composition with the anti-aging effect provided by the invention has the effects of resisting mitochondrial aging injury, enhancing the body immunity function, compensating genetic genes of senile cells, resisting neuroendocrine decline and scavenging free radicals and can systematically and comprehensively resist the skin aging in a synergetic manner; and moreover, not only aiming at a certain link problem of aging, the composition further relieves aging symptoms such as increased skin wrinkles, loose skin, skin yellowing, coarse pores, dryness, stains and the like. In addition, the composition used by the invention is a natural, green, healthy and safe pure plant composition, does not have any toxic or side effect, is stable in properties, is easily absorbed by the skin and can allow the skin to return to a natural state.

The invention discloses a method for efficiently acquiring a gerbera jamesonii detoxification seedling. The method is characterized by comprising the following steps: (1) collecting gerbera jamesonii tender leaves, and de-differentiating the gerbera jamesonii to generate callus; (2) thermally treating the callus, continuously inducing the callus to form adventitious bud, and rooting the adventitious bud to form a strong tissue culture seedling; (3) further stripping a tissue culture seedling stem tip, and de-differentiating to generate callus; (4) thermally treating the callus, and inducing to form a seedling; (5) randomly extracting the tissue culture seedling in the step (4), carrying out the virus detection, if the seedling is qualified in detection, the seedling is the gerbera jamesonmii detoxification seedling, if the seedling is not qualified in the detection, repeating the steps (1) to (4) until the qualified gerbera jamesonii detoxification seedling is acquired. The callus is generated by virtue of the twice de-differentiation, and then the callus is thermally treated and de-differentiated to finally obtain the gerbera jamesonii detoxification seedling, so that the difficulty of the existing detoxification technology such as non-thoroughness in detoxification, complexity in operation and can meet the factory-like detoxification production requirement.

This invention discloses the pretreatment liquor for Africa mum. The character: the pretreatment liquor consists thiabendazole, citric acid, 6-benzyl purine, hyposulphite, silver nitrate and qulatong soluted in water in proportion. Using the pretreatment liquor of This invention discloses good for the come out of Africa mum flowers and delayed the withering of flowers.

The invention discloses a seedling raising method of gerbera jamesonii haploid transplanted seedlings. The method mainly comprises the steps that gerbera jamesonii haploid buds are transferred into aproliferation culture medium for subculture many times, and when the proliferation cardinal number reaches a required number, the gerbera jamesonii haploid buds are sequentially transferred into a strong seedling culture medium and a rooting culture medium for culture; when 4-12 roots grow from base parts of plants, culture bottles are transferred into a greenhouse and stay for 4 days, the sterilized rooting seedlings are transferred into seedling raising bags and grow for 50 days, and the gerbera jamesonii haploid transplanted seedlings are obtained, wherein the greenhouse is covered with a shading net with the shading rate of 70%. After subculture, strong seedling culture is carried out, so that healthy and easy rooting of gerbera jamesonii haploids are promoted; before transplanting, the seedlings are placed in the greenhouse, so that the environment adaptability of the rooting seedlings is enhanced; through seedling selection before transplanting, plant sterilization and timely pesticide spraying after transplanting, and the seedling hardening survival rate and transplanting survival rate of gerbera jamesonii haploid plants are improved. The good-quality transplanted seedlingsare provided for gerbera jamesonii haploid plant cultivation.

The invention provides a cultivating method of a ball-shaped gerbera coenospecies, comprising the following steps of: selecting parents; pollinating crossly; managing the growth of a pollinated maternal plant till a first generation seed is gained; sowing the first generation seed till a plantlet grows; transplanting the plantlet till an anthesis; respectively selecting single plants with high polyphyll degree as a female parent and a male parent; collecting the pollen of multiple male parent single plants, uniformly mixing, and then pollinating crossly to obtain a second generation seed; sowing the second generation seed till the plantlet grows; selecting a single plant of ball-shaped gerbera when the plantlet is transplanted till the anthesis, and using a flower bud with an unexposed core as an explant so as to obtain a tissue culture plantlet of the stable ball-shaped gerbera coenospecies with great high ornamental value through induction cultivation, rooting cultivation and transition cultivation. The cultivating method is an effectively method for cultivating the ball-shaped gerbera coenospecies.

The invention discloses a cultivation method of a new variety of barberton daisy, and belongs to the technical field of cultivation of new plant varieties. The new variety of barberton daisy is cultivated by male parent introduction, male parent material treatment, pollination and cultivation management, single plant selection and plant line formation and plant line character evaluation. A new wayis provided for germplasm introduction in breeding work, 2-3 times of pollen collection of the male parent can be reached, the number of pollen collection and multiple pollination times are increased, the seed setting rate of cross pollination is increased by 15%-20%, the number of individual plant selection groups is increased, and cultivation efficiency of the new variety is improved.

The invention provides a formula for beef head seasoning and a preparation method thereof, including main ingredients and auxiliary materials, the main ingredient is fresh beef head, and the materials of the auxiliary materials include purified water, aniseed, Chinese prickly ash, cassia bark, white buckle, Cumin, cloves, Amomum, Angelica dahurica, Xiangyi, Luo Han Guo, Tsao Guo, fragrant leaves, fragrant fruit, grass buckle, meat buckle, fresh ginger, green onion, pepper, salt, the auxiliary material formula is aimed at the production method of beef head, The materials used to make the beef head in the present invention can not only taste the taste as soon as possible, but also have a unique taste, stewed slowly over low heat, and the meat is delicious. The role of cancer and anti-cancer, the content of various nutrients in the cow head and auxiliary materials is balanced. Collagen can enhance the ability of the human body to make cells. It is suitable for people after surgery or anemia. For the elderly, it can slow down the blood and Qi. It is beneficial to improve the immunity of teenagers and enhance memory.

The invention relates to spleen-refreshing and child-nourishing granules, and a preparation method and quality control method thereof. The spleen-refreshing and child-nourishing granules are preparedaccording to the following method: taking sowthistle tasselflower herb, pilose gerbera herb, pittosporm roots and rhizomes of broadleaf common valeriana according to a prescription amount, adding warmwater at 40-60 DEG C for soaking for 0.5h, performing decocting twice, combining decoctions, performing filtering, concentrating the obtained filtrate to a clear paste with a relative density of 1.15-1.18 (80 DEG C), adding auxiliary materials, and performing granulation. The method can sufficiently retain active ingredients. The invention also discloses a quality control method for the spleen-refreshing and child-nourishing granules, and the method can better control the product quality.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine formula for treating a lung cancer. The traditional Chinese medicine is prepared from the following traditional Chinese medicinal materials in parts by weight: 15-20 parts of pinellia ternate, 15-20 parts of mangnolia officinalis, 10-15 parts of rhizoma arisaematis, 15-20 parts of whitebackleaf mallotus roots, 15-20 parts of calamus, 10-15 parts of small diuranthera roots, 5-10 parts of gmelin sealavender herb, 10-15 parts of fructus evodiae, 10-15 parts of common perilla, 10-15 parts of fiveleaf akebia fruits, 10-15 parts of pericarpium citri reticulatae, 15-20 parts of draba nemorosa, 10-15 parts of arisaema cum bile, 10-15 parts of gerbera delauayi franch, 10-15 parts of curcuma aromatic and 5-10 parts of rhizoma corydalis. The traditional Chinese medicine formula has the advantages that the used traditional Chinese medicine accords with the regulation of the Chinese pharmacopoeia, and is safe and reliable; a plurality of medicines are combined to reach the efficacies of eliminating dampness, eliminating phlegm, tonifying the spleen, regulating qi and the like; and the traditional Chinese medicine has the characteristics of being accurate in curative effect on the lung cancer caused by phlegm-damp obstructing the lung, fast in acting, high in recovery rate, free of a side effect and the like.

The invention relates to a traditional Chinese medicine composition, in particular to a traditional Chinese medicine preparation for treating diarrhea. The traditional Chinese medicine preparation is prepared from, by weight, 10-15 parts of roots of maire sophora, 8-10 parts of berchemia sinica, 10-15 parts of herb of pilose gerbera, 8-15 parts of fresh juteleaf raspberry roots, 10-13 parts of roots of java campanumoea, 6-10 parts of herb of common evolvulus, 5-8 parts of tikoua fig roots, 6-15 parts of polygonum glabrum willd, 9-12 parts Fengshugen, 10-16 parts of pomegranate roots, 9-15 parts of herba houttuyniae, 6-12 parts of dried tangerine peel, 9-15 parts of medicated leaven, 8-12 parts of agrimony and 6-12 parts of membranes of chicken gizzard. The traditional Chinese medicine for treating the diarrhea takes fructus amomi and semen coicis as monarch drugs, other adjuvants are matched, all the medicines synergistically act with one another, the effects of clearing heat, removing toxicity, cooling blood, stopping diarrhea, warming the middle-jiao, strengthening the spleen, removing heat and dampness and relieving pain and inflammation are achieved, a good therapeutic effect on patients suffering from the diarrhea is good, and the traditional Chinese medicine preparation is safe and free of side effect.

The invention discloses a method for obtaining an African daisy regeneration plant through inducing a somatic embryo. The method comprises the steps that an African daisy receptacle serving as an explant material is cultured in a somatic embryo inducing culture medium containing 6-benayl aminopurine, kinetin, thidiazuron, naphthylacetic acid, picloram and casein hydrolysate; the somatic embryo is induced; after the somatic embryo germinates, the somatic embryo is subjected to successive transfer culture in a differential medium; and the regeneration plant is obtained. According to the method, the African daisy receptacle serves as an explant, and the regeneration plant is obtained through a somatic embryo approach without a callus stage, so that a situation that explant is mutated due to dedifferentiation inducing callus regeneration can be avoided. By utilizing the method to perform African daisy tissue culture for seedlings, the culture procedure is simplified, the culture cycle is shortened, the quality of the seedlings is improved, and base materials are provided for a large number of high-quality seedlings by in-vitro propagation of African daisy and transgenic research.

The invention provides a Chinese herba preparation for curing plasma cell mastitis and a preparation method thereof. The Chinese herba preparation is prepared from the following raw herbal materials: deer-horn glue, baked ginger, waltheria indica, polygonum runcinatum, gerbera, smilacina japonica, blue gum, indigofera trifoliata, celtis biondii, canescent wikstroemia, clerodendrum bungei, hypericum sampsonii hance, phaius tankervilleae, dasiphorafruticosa flower, herb of wilson yarrow, watter milkwort root leaf or bark, ficus sarmentosa, herb of klein cinquefoil, herb of obcordate christia, flower of operculate cleistocalyx, phyllanthus virgatus, all grass of pendulousflower elsholtzia and selaginella doederleinii hieron. The Chinese herba preparation has the beneficial effects of being used for plasma cell mastitis, and being convenient to use, high in efficiency, free of toxic and side effects, low in relapse possibility and simple in preparation process.