281 results about "Coumarin" patented technology

The present invention relates to a preparation method of an intelligent self-repairing, flame-retardant and light-resistant water-based polyurethane coating. The method comprises the following steps: preparing a self-made hyperbranched flame retardant and using it in the synthesis of a polyurethane prepolymer; cooling the mixture to 58° C., adding 2,2-dimethylolpropionic acid, reacting the mixture for 1 to 2 hours, adding 1 to 2 parts of a disulfide compound, reacting the mixture for 1 to 2 hours at 60 to 80° C., adding 1 to 2 parts of a hydrogen bonding agent, reacting the mixture for 1 hour at 60 to 80° C., adding 0.5 to 1.5 parts of a coumarin derivative, and reacting the mixture for 60 to 90° C. The mixture was stirred for 1-2 hours, and then spiropyran, diethanol monoisopropanolamine, and 0.01 parts of p-toluenesulfonamide were added, and the mixture was reacted at 50°C for 30 minutes. The temperature was lowered to 40-45°C, and 1-2 parts of triethylamine were added, and the mixture was reacted for 1-2 hours. The mixture was cooled to room temperature, and 60-65 parts of water were added. The mixture was stirred at a speed of 1800-2000 r / min for 0.5-1 hour to obtain an intelligent self-healing, flame-retardant, and light-resistant water-based polyurethane coating. The flame retardancy and self-healing properties of the obtained water-based polyurethane were significantly improved.

The invention discloses a pyridone type coumarin derivative as well as a preparation method and application thereof. The invention autonomously designs and synthesizes a novel pyridone type coumarin derivative. Experimental results show that the pyridone type coumarin derivative has a remarkable inhibition effect on KYSE-70 and KYSE-150 cells, and the pyridone type coumarin derivative has a very good application value in anti-inflammatory, anti-virus, antibacterial or anti-cancer aspects.

The invention discloses a plant extraction and purification method of glycyrrhizic coumarin, and belongs to the technical field of natural product extraction. The method comprises a purification method suitable for industrial production of glycyrrhizic coumarin and a purification method for laboratory preparation of glycyrrhizic coumarin. According to the laboratory purification method, licorice extract sequentially passes through a silica gel column and a C18 column for coarse separation and then is refined through semi-preparative liquid chromatography, the glycyrrhizic coumarin with the purity higher than 98% is obtained and can be used as a standard substance for licorice quality research, and an important basis is provided for further deep research and development of glycyrrhizic coumarin. According to the present invention, the industrial production method is based on the laboratory extraction step, the steps are simplified, such that the method is suitable for the pilot scale production, the steps are simple, the efficiency is high, the yield is high, the glycyrrhizic coumarin with the purity of more than 98% can be obtained through the separation, and the advantages are provided for the further research and the wide application of the glycyrrhizic coumarin.

The invention provides a coumarin derivative containing oxadiazole (thiadiazole) thioether and a preparation method and application thereof, the structural general formula of the coumarin derivative containing oxadiazole (thiadiazole) thioether is shown as I, and the structural general formula of the coumarin derivative containing thiadiazole thioether is shown as II, the synthesis route of the coumarin derivative containing oxadiazole (thiadiazole) thioether is simple and practical, the preparation raw materials are easy to obtain, the cost is low, and the prepared compound has a good control effect on plant viruses including tobacco mosaic virus, cucumber mosaic virus, potato Y virus, southern rice black-streaked dwarf virus or rice stripe virus. According to the invention, a favorable lead compound is provided for the creation of a natural bionic antiviral agent; # imgabs0 #

The invention discloses epoxy vinyl ultraviolet curing resin and a preparation method thereof, and belongs to the technical field of epoxy resin materials.The preparation method comprises the following steps that fluorine-containing alicyclic epoxy resin, thiol-alkene functionalized vinyl acid, coumarin modified styrene, a prepolymer, an active prepolymer and nanoscale dispersion liquid are sequentially prepared; and carrying out nano synergistic modification, thermal activation component introduction, gradient photo-thermal synergistic curing and super-hydrophobic surface optimization to obtain the epoxy vinyl ultraviolet curing resin. According to the invention, through ternary compounding of coumarin modified styrene, thiol-alkene functionalized vinyl acid and fluorine-containing alicyclic epoxy resin and synergistic effect of the three components and a gradient photo-thermal curing process, the problems of excessive cross-linking brittleness of a surface layer, insufficient curing and weak bonding of an inner layer, permeation of a medium along defects and the like are synchronously solved; systematic improvement of strength, cohesive force and corrosion resistance is achieved.

The invention discloses a probe Cvs-B-NHS based on a coumarin derivative as well as a preparation method and application of the probe Cvs-B-NHS. According to the fluorescent probe Cvs-B-NHS of the coumarin derivative, a coumarin mother nucleus and succinimide ester are organically combined, the excellent fluorescence characteristic of coumarin is reserved, efficient protein labeling capacity is achieved, high-sensitivity fluorescence signal output and specific labeling of protein are achieved, and the fluorescent probe Cvs-B-NHS has the advantages of being high in sensitivity and high in sensitivity. And the accuracy and reliability of protein detection and imaging are improved. The invention discloses a TDP-43 protein phase separation labeling method based on a Cvs-B-NHS probe, which provides a new means for researching the action mechanism of a TDP-43 (G335D) mutant in neurodegenerative diseases, and is beneficial to promoting the research progress in the field of neurodegenerative diseases. The application of the Cvs-B-NHS probe in evaluating the polarity change of the microenvironment in the protein liquid-liquid phase separation process in the cytoplasm environment caused by mitochondrial signal disturbance provides a powerful tool for the research of the protein phase separation microenvironment.

The invention discloses a double-targeting probe and a preparation method and application thereof.The structure of the double-targeting probe is shown in the formula I. The double-targeting probe has the beneficial effects that SO2 can be monitored in the cell apoptosis process, the dynamic change of SO2 between mitochondria and lipid droplets in cells can be tracked, the core structure of the probe MLR is based on a coumarin part, and the core structure of the probe MLR can be used for detecting the apoptosis of the cells. The probe MLR is connected with a benzopyran cation structure specially designed for detecting SO2, SO2 generated in the death process of copper enables charges of a cation part in the probe MLR to be redistributed, a Michael addition reaction of SO2 and carbon-carbon double bonds is triggered, a fluorescence signal of the probe is changed, and the fluorescence signal is detected. The design of the probe provides a new tool for monitoring key biochemical changes in the copper death process, and is helpful for deeply understanding the molecular mechanism of copper death.

The present invention discloses the application of coumarin-3-carboxylic acid in preventing and treating plant bacterial diseases. The coumarin-3-carboxylic acid provided by the present invention has a significant inhibitory effect on plant bacterial diseases. The plant bacterial diseases are oryzae bacterial leaf blight, oryzae bacterial leaf streak, citrus canker, cantaloupe bacterial fruit spot, tomato bacterial wilt, strawberry bacterial angular leaf spot, cabbage black rot, cabbage soft rot, potato soft rot, mango bacterial angular leaf spot, cassava bacterial wilt, cucumber bacterial angular leaf spot, kiwifruit canker and banana bacterial soft rot. The coumarin-3-carboxylic acid provided by the present invention has protective and therapeutic effects on melon bacterial fruit spot.

The invention discloses a coumarin-based bio-based epoxy resin monomer and a preparation method and application thereof, and the structure of the monomer is DPE, DCE and DHE. The monomer takes dihydrocoumarin and biomass amine compounds (including putrescine, cadaverine and 1, 6-hexamethylenediamine) as raw materials, biomass bisphenol DP, DC and DH are prepared through a simple ring-opening reaction, and then the monomer is prepared through a glycidyl reaction. A bisamide structure is innovatively introduced into the epoxy resin monomer, so that the epoxy resin monomer has the advantages of simple and convenient synthesis process, high yield, easily available raw materials, good biological safety and the like, and the performance is superior to that of traditional petroleum-based epoxy resin. The bio-based epoxy resin prepared based on the monomer shows a novel molecular structure and excellent mechanical properties and heat resistance.

The present invention belongs to the fields of chemistry, materials science, and corrosion engineering, and discloses a fluorescent metal corrosion inhibitor based on coumarin and furan heterocycles, as well as its preparation method and application. A simple and efficient Schiff base reaction is used to introduce a five-membered furan ring into the fluorophore coumarin. The Schiff base carbon-nitrogen double bond and the furan heterocycle, as well as the oxygen heteroatom of the fluorophore coumarin itself, are utilized to coordinate well with iron ions. This not only achieves metal corrosion inhibition, but also makes it possible to detect the amount of corrosion inhibitor used through fluorescence, avoiding environmental pollution caused by excessive use. The fluorescent metal corrosion inhibitor has an inhibition concentration of 7×10 ‑5 mol / L, it exhibits the best corrosion inhibition effect on carbon steel, and the corrosion inhibition efficiency can reach 73.25%.

This invention discloses a strain of *Neocallimastix* LGM-ZA9 and its application in the preparation of a coumarin-degrading feed additive. *Neocallimastix* LGM-ZA9 is classified as *Neocallimastix* sp., with accession number CGMCC No. 17592, and its optimal growth temperature is 39℃. The fermentation broth of *Neocallimastix* LGM-ZA9 can be used to degrade the anti-nutritional factor coumarin. The method is simple: the coumarin-containing material to be degraded is mixed with the fermentation broth, sterilized, and then the fermentation broth is added for anaerobic fermentation to degrade the coumarin. This preparation method is simple, highly operable, and can significantly reduce the coumarin content, providing a new method for feeding ruminants with sweet clover as a high-quality forage.

The present invention belongs to the field of medicinal chemistry, and specifically relates to coumarin-chalcone hybrid derivatives as phosphodiesterase 2 inhibitors and their applications. The present invention expands hybrid derivatives based on the structures of coumarin and chalcone, provides novel small molecule compounds that are inhibitors of PDE2, and the obtained compounds have good PDE2 inhibitory activity. These compounds have the potential to treat central nervous system diseases such as memory defects, cognitive impairments, anxiety, and depression, and can be used as active ingredients to prepare drugs that inhibit the activity of PDE2. Moreover, the preparation is easy, the reaction is rapid, the yield is high, the post-treatment is simple, and the solid acid catalyst left after post-treatment filtration can still be recycled, greatly reducing the reaction time and experimental cost.

The invention discloses a coumarin-thiosemicarbazide compound and application of the coumarin-thiosemicarbazide compound. The preparation method comprises the following steps: firstly, synthesizing a coumarin-thiosemicarbazide derivative, and carrying out in-vitro antioxidant activity screening to obtain preferable derivatives 4g and 4i; analyzing an antioxidant action target based on network pharmacology and molecular docking, and performing intersection mapping on the action target and the antioxidant target of the compounds 4g and 4i to obtain a common target; constructing a PPI interaction network, and screening to obtain potential core targets; carrying out GO function and KEGG pathway enrichment analysis on the core target spot to obtain a related biological process and signal pathway; and carrying out molecular docking operation on the key target spot and compounds 4g and 4i to verify the binding capacity of the compounds and the target spot. Potential antioxidant targets and modern biological mechanisms of the derivatives 4g and 4i are preliminarily revealed by using a network pharmacology method, and important guidance and basis are provided for development of antioxidant drugs.

The invention discloses an ultraviolet-controlled photo-reversible dynamic crosslinking high polymer material with reversible antibacterial, shape deformation and toughening functions, and a preparation method thereof, belonging to the technical field of high polymer materials. Comprising the following steps: step 1) dissolving coumarin-3-formic acid, three-arm PCL (polycaprolactone), dicyclohexylcarbodiimide and 4-dimethylaminopyridine in dry dichloromethane in an inert atmosphere to obtain a reaction mixture; the invention relates to a preparation method of a coumarin-terminated three-arm polycaprolactone cross-linked polymer material.The preparation method comprises the following steps of (1) preparing coumarin-terminated three-arm polycaprolactone, (2) heating a reaction mixture obtained in the step (1) for set time, and filtering and separating an obtained product to obtain coumarin-terminated three-arm polycaprolactone, and (3) cross-linking and curing for set time through 365 nm ultraviolet to obtain a target product cross-linked polymer material.The function is highly integrated and dynamically adjustable, and the limitation that a traditional material is single in function is broken through.

Provided are a pyrrolo[h]coumarin dye and a use thereof in sequencing. Specifically provided are a compound represented by formula I or a stereoisomer, tautomer, crystal form, salt, ester or solvate thereof. The compound has the advantages of high brightness and high stability. The compound exhibits strong fluorescence under blue light excitation. When used for labeling dNTPs and then applied in gene sequencing, the sequencing signal, resolution and throughput are all significantly improved, and the sequencing quality is significantly improved.

The invention discloses a preparation method of a 3-substituted isocoumarin compound, and belongs to the technical field of coumarin compound synthesizing.The preparation method comprises the steps that a 2-bromophenylboronic acid compound and alkyne serve as reaction substrates, a bidentate nitrogen ligand is added and dissolved in a solvent, under the action of alkali and a copper catalyst, a normal-pressure one-step cyclization reaction is conducted in the CO2 atmosphere, and the 3-substituted isocoumarin compound is obtained. The 3-substituted isocoumarin compound is obtained. According to the method, CO2, alkyne and 2-bromophenylboronic acid are used as reaction raw materials, cheap copper is used as a catalyst, the use amount is small, the 3-substituted isocoumarin is obtained through carbon-carbon coupling-cyclization by a CO2 activation one-step method under normal pressure, the conditions are mild, the cost is low, and the method is environmentally friendly.

This invention belongs to the field of lubrication technology, specifically relating to a method for simultaneous online detection of grease distribution and oil separation. In this invention, suitable fluorescent agents are selected: methylpyrrole is soluble only in the base oil phase, and coumarin is soluble only in the thickener phase. The distribution of the base oil is characterized by detecting the fluorescence intensity of methylpyrrole, and the distribution of the thickener is characterized by detecting the fluorescence intensity of coumarin. Methylpyrrole and coumarin can be excited simultaneously by the same light source, thus generating fluorescence synchronously. The wavelength difference between the fluorescence emission peaks of methylpyrrole and coumarin is significant; therefore, the two fluorescence signals can be separated using a spectrometer, allowing two receivers to simultaneously receive both fluorescence signals.

The application belongs to the technical field of pharmaceutical chemistry, and particularly relates to a coumarin derivative with anti-breast cancer activity and application thereof. The chemical structural formula of the coumarin derivative is shown in formula (I) or formula (II), wherein R1 is an electron-withdrawing or electron-donating group, and R2 is a saturated nitrogen heterocycle or an aromatic nitrogen heterocycle. The coumarin analog prepared by the application has a certain inhibitory effect on breast cancer cells.

The invention belongs to the field of pesticides, and particularly relates to a phenylamidine compound containing a coumarin structure or a pesticide acceptable salt, a composition and application of the phenylamidine compound, the compound has the structure shown in the formula (I), and all groups in the formula are defined in the specification. According to the compound disclosed by the invention, a substituted coumarin structure is introduced on the basis of an amidine structure, and the compound capable of being used as a bactericide is developed and obtained and has very outstanding biological activity and crop safety.

The invention discloses a preparation method and application of aerogel for detecting and adsorbing aluminum ions, and belongs to the technical field of sewage treatment. According to the method, a halloysite nanotube is taken as a matrix, and the HNT-KC probe with specific fluorescence response to aluminum ions is synthesized through silanization, acylating chlorination and gradual reaction with a coumarin derivative and furohydrazide; meanwhile, preparing aldehyde-terminated polyethylene glycol as a cross-linking agent; finally, the probe, a chitosan solution and an aldehyde-terminated polyethylene glycol solution are subjected to co-gelation through a Schiff base reaction, and the composite aerogel is prepared through dehydration aging and freeze drying. The aerogel integrates fluorescence detection and adsorption removal functions, has the advantages of high specific surface area, porous structure, high sensitivity, strong selectivity and good stability, can realize rapid detection and efficient removal of aluminum ions in a water body, is green in raw materials and simple in process, and has a wide application prospect in the field of water treatment.

The invention provides a method for removing coumarin in natural perfume. The method comprises the following steps: dissolving the natural perfume by using a solvent, adding an alkaline substance to adjust the pH value, carrying out a heating reflux reaction, concentrating after the reaction, extracting a concentrate by using an organic solvent, and carrying out reduced pressure distillation on extract liquor to remove the organic solvent, thereby obtaining the coumarin-removed natural perfume. By adopting the method for removing the coumarin in the natural perfume, the coumarin in the natural perfume such as hierochloe odorata extract, sweet clover extract, grain rain grass extract, cassia oil, pyrola calliantha extract and fenugreek tincture can be effectively removed, the coumarin content of the obtained coumarin-removed natural perfume product is less than 0.5 mu g / g, and the safety and application range of the product are greatly improved. The preparation method is simple in operation process, low in cost and very suitable for industrial production.

The invention belongs to the field of chemical analysis and testing, and particularly relates to an aminobenzaldehyde-based coumarin fluorescent probe for preparing a reagent for detecting mercaptosuccinic acid and a preparation method of the aminobenzaldehyde-based coumarin fluorescent probe. The preparation method comprises the following steps: dissolving 2-aminobenzaldehyde and 7-(diethylamino) coumarin-3-formic acid in dichloromethane, adding 1-ethyl-(3-dimethylamino aldehyde) carbodiimide hydrochloride (EDCI) and 1-hydroxybenzotriazole (HBOT) as condensing agents, carrying out a stirring reaction at room temperature, carrying out separation and purification on the obtained crude product through a thin-layer chromatography, and carrying out recrystallization to obtain the coumarin derivative. The final yellow product, namely the aminobenzaldehyde-based coumarin fluorescent probe Y-SH, is obtained. The coumarin fluorescent probe prepared by the preparation method disclosed by the invention can be used for rapidly detecting mercaptosuccinic acid in deionized water.

The invention provides nucleic acid base composite nano-particles and a preparation method thereof. The preparation method comprises the following steps: taking PDMA and an acrylate adenine monomer as raw materials, carrying out polymerization to obtain a diblock polymer, taking the diblock polymer and a coumarin monomer as raw materials, carrying out polymerization to obtain PA, and assembling the PA into a nucleated cross-linked nano-micelle; pDMA and an acrylate thymine monomer are used as raw materials and polymerized to obtain a block polymer, and the block polymer is assembled into the nano-micelle; and mixing the core cross-linked nano-micelles with the nano-micelles, and interacting to obtain the composite nano-particles. According to the preparation method disclosed by the invention, the core-crosslinked nano-micelle containing adenine is constructed, and the nano-micelle has poly (N, N-dimethylacrylamide) with a hydrophilic block, and the poly (N, N-dimethylacrylamide) can stably exist in an aqueous solution; and mixing the core-crosslinked nano-micelles with the nano-micelles to obtain the composite nano-particles through biologically excited nuclear-base interaction.

This invention discloses a coumarin-linked conjugated porous polymer, its preparation method, and its applications. The preparation method includes: subjecting a homogeneous mixture containing o-hydroxybenzaldehyde monomer, phenylacetonitrile monomer, solvent, and catalyst to a Knauven-Gail condensation reaction to obtain the coumarin-linked conjugated porous polymer. The coumarin used in this invention to prepare the coumarin-linked conjugated porous polymer is a highly planar molecule. Its full conjugation extends the absorption wavelength to the visible light region and enhances π-electron excitation, thus exhibiting strong photosensitivity. The resulting series of coumarin-linked highly conjugated nanoarray conjugated porous polymers possess high porosity and a fully conjugated structure, making them highly efficient for applications in photocatalysis, fluorescence sensing, and optoelectronic devices.

The invention relates to the technical field of traditional Chinese medicine detection, in particular to a method for determining the content of various components in blumea balsamifera oil. According to the method, a gas chromatography-mass spectrometry (GC-MS) technology is adopted, and chromatographic conditions are as follows: a chromatographic column is an SH-Stabilwax (30m * 0.32 mm, and the film thickness is 0.25 [mu] m) capillary column; the temperature of a sample inlet is 230 DEG C; the split ratio is 10: 1; the sample size is 1 microliter; the mass spectrum conditions are as follows: the ion source temperature is 200 DEG C; the interface temperature is 230 DEG C; the ionization mode is electron impact ion (EI); the detection voltage is 0.2 KV; the solvent is delayed for 2 minutes; the mass number scanning range is 30 to 400 amu; and SIM mode. According to the method disclosed by the invention, the contents of alpha-pinene, camphene, beta-pinene, limonene, p-cymene, camphor, alpha-coumarin, linalool, bornyl acetate, beta-caryophyllene, myrtenal, alpha-caryophyllene, L-borneol, perillaldehyde, caryophyllene oxide, guaiacol, beta-eudesmol and xanthoxylin in the blumea balsamifera oil can be simultaneously determined; the method provided by the invention has good precision, repeatability and stability, and the quality consistency of the blumea balsamifera oil can be quickly, accurately and effectively evaluated through detection of multiple components.

The present invention discloses coumarin-3-carboxamide derivatives, their preparation methods, and their use in the preparation of anti-tumor drugs. The coumarin-3-carboxamide derivatives or pharmaceutically acceptable salts thereof have the structural formula shown in Formula (I): wherein R1 is hydrogen, an alkoxy group, or an amine group; R2 is hydrogen or a halogen atom; and m and n are 0 to 5. This invention is based on the development potential and value of coumarin derivatives in tumor treatment. The inventors of the present invention have studied coumarin-based anti-tumor drugs and discovered a series of coumarin derivatives with outstanding anti-tumor activity that can be used to treat breast cancer, oral cancer, or cervical cancer. #imgabs0#

The present invention provides a separation membrane for an electrochemical device, which includes a porous polymer substrate; and a porous coating layer formed on at least one surface of the porous polymer substrate, wherein the porous coating layer includes inorganic particles whose surface is modified with one or more compounds selected from the group consisting of coumarin and its derivatives, and a polymer binder, and is characterized by removing radicals or transition metals generated during the charge and discharge process of the electrochemical device to prevent performance degradation.

The method of obtaining modified carbon quantum dots by N-substitution of compounds from the coumarin group, consists, according to the invention, in the fact that the carbon quantum dots, obtained by carbonisation of biomass constituting, inter alia, 1 biologically active substance having free carboxyl groups, are modified by the formation of an amide bond in an aqueous medium, under normal conditions, and then purified using dialysis membranes. During grafting, the ratio of dye to EDC is 1 to 1.1. The invention also relates to the carbon quantum dots with a fluorescence emission spectrum in the range 380- 800 nm, obtained by this method.

The application discloses a preparation method of a temperature-ultraviolet light double response type functional polymer, specifically comprising the following steps: preparing a macromolecular initiator PEG2-ABCPA; preparing a p-styrylmethyl diethanolamine borate monomer VMAB; and performing a free radical polymerization reaction on the macromolecular initiator PEG2-ABCPA, the p-styrylmethyl diethanolamine borate monomer VMAB and 7-(4-vinylbenzyloxy)-4-methyl coumarin VBC to obtain a block copolymer, i.e. the temperature-ultraviolet light double response type functional polymer. The polymer not only has unique amphiphilic properties, but also exhibits double response to temperature and ultraviolet light, thereby showing wide application prospects in the fields of drug controlled release, intelligent materials, photoelectric devices and the like.

The invention relates to the technical field of heavy metal detection, in particular to a preparation method of a dicoumarin-based fluorescent probe and application of the dicoumarin-based fluorescent probe in palladium detection, the fluorescent probe is synthesized by taking F, Cl and Br substituted dicoumarin as a fluorophore and allyl formate as a recognition group, and when palladium is added into the probe, obvious fluorescence appears, and the fluorescence is detected. The detection limit can reach 55nmol / L at the lowest, and the method has high selectivity in vivo and in vitro, realizes the purpose of rapid and efficient detection of palladium, and effectively solves the problems of expensive detection instruments, tedious operation, high personnel requirements and the like in palladium detection by using the technical scheme.