193 results about "Coumarin" patented technology

The invention discloses a double-targeting probe and a preparation method and application thereof.The structure of the double-targeting probe is shown in the formula I. The double-targeting probe has the beneficial effects that SO2 can be monitored in the cell apoptosis process, the dynamic change of SO2 between mitochondria and lipid droplets in cells can be tracked, the core structure of the probe MLR is based on a coumarin part, and the core structure of the probe MLR can be used for detecting the apoptosis of the cells. The probe MLR is connected with a benzopyran cation structure specially designed for detecting SO2, SO2 generated in the death process of copper enables charges of a cation part in the probe MLR to be redistributed, a Michael addition reaction of SO2 and carbon-carbon double bonds is triggered, a fluorescence signal of the probe is changed, and the fluorescence signal is detected. The design of the probe provides a new tool for monitoring key biochemical changes in the copper death process, and is helpful for deeply understanding the molecular mechanism of copper death.

The invention discloses a coumarin-based bio-based epoxy resin monomer and a preparation method and application thereof, and the structure of the monomer is DPE, DCE and DHE. The monomer takes dihydrocoumarin and biomass amine compounds (including putrescine, cadaverine and 1, 6-hexamethylenediamine) as raw materials, biomass bisphenol DP, DC and DH are prepared through a simple ring-opening reaction, and then the monomer is prepared through a glycidyl reaction. A bisamide structure is innovatively introduced into the epoxy resin monomer, so that the epoxy resin monomer has the advantages of simple and convenient synthesis process, high yield, easily available raw materials, good biological safety and the like, and the performance is superior to that of traditional petroleum-based epoxy resin. The bio-based epoxy resin prepared based on the monomer shows a novel molecular structure and excellent mechanical properties and heat resistance.

This invention discloses a strain of *Neocallimastix* LGM-ZA9 and its application in the preparation of a coumarin-degrading feed additive. *Neocallimastix* LGM-ZA9 is classified as *Neocallimastix* sp., with accession number CGMCC No. 17592, and its optimal growth temperature is 39℃. The fermentation broth of *Neocallimastix* LGM-ZA9 can be used to degrade the anti-nutritional factor coumarin. The method is simple: the coumarin-containing material to be degraded is mixed with the fermentation broth, sterilized, and then the fermentation broth is added for anaerobic fermentation to degrade the coumarin. This preparation method is simple, highly operable, and can significantly reduce the coumarin content, providing a new method for feeding ruminants with sweet clover as a high-quality forage.

The invention discloses an ultraviolet-controlled photo-reversible dynamic crosslinking high polymer material with reversible antibacterial, shape deformation and toughening functions, and a preparation method thereof, belonging to the technical field of high polymer materials. Comprising the following steps: step 1) dissolving coumarin-3-formic acid, three-arm PCL (polycaprolactone), dicyclohexylcarbodiimide and 4-dimethylaminopyridine in dry dichloromethane in an inert atmosphere to obtain a reaction mixture; the invention relates to a preparation method of a coumarin-terminated three-arm polycaprolactone cross-linked polymer material.The preparation method comprises the following steps of (1) preparing coumarin-terminated three-arm polycaprolactone, (2) heating a reaction mixture obtained in the step (1) for set time, and filtering and separating an obtained product to obtain coumarin-terminated three-arm polycaprolactone, and (3) cross-linking and curing for set time through 365 nm ultraviolet to obtain a target product cross-linked polymer material.The function is highly integrated and dynamically adjustable, and the limitation that a traditional material is single in function is broken through.

Provided are a pyrrolo[h]coumarin dye and a use thereof in sequencing. Specifically provided are a compound represented by formula I or a stereoisomer, tautomer, crystal form, salt, ester or solvate thereof. The compound has the advantages of high brightness and high stability. The compound exhibits strong fluorescence under blue light excitation. When used for labeling dNTPs and then applied in gene sequencing, the sequencing signal, resolution and throughput are all significantly improved, and the sequencing quality is significantly improved.

The invention discloses a preparation method of a 3-substituted isocoumarin compound, and belongs to the technical field of coumarin compound synthesizing.The preparation method comprises the steps that a 2-bromophenylboronic acid compound and alkyne serve as reaction substrates, a bidentate nitrogen ligand is added and dissolved in a solvent, under the action of alkali and a copper catalyst, a normal-pressure one-step cyclization reaction is conducted in the CO2 atmosphere, and the 3-substituted isocoumarin compound is obtained. The 3-substituted isocoumarin compound is obtained. According to the method, CO2, alkyne and 2-bromophenylboronic acid are used as reaction raw materials, cheap copper is used as a catalyst, the use amount is small, the 3-substituted isocoumarin is obtained through carbon-carbon coupling-cyclization by a CO2 activation one-step method under normal pressure, the conditions are mild, the cost is low, and the method is environmentally friendly.

This invention belongs to the field of lubrication technology, specifically relating to a method for simultaneous online detection of grease distribution and oil separation. In this invention, suitable fluorescent agents are selected: methylpyrrole is soluble only in the base oil phase, and coumarin is soluble only in the thickener phase. The distribution of the base oil is characterized by detecting the fluorescence intensity of methylpyrrole, and the distribution of the thickener is characterized by detecting the fluorescence intensity of coumarin. Methylpyrrole and coumarin can be excited simultaneously by the same light source, thus generating fluorescence synchronously. The wavelength difference between the fluorescence emission peaks of methylpyrrole and coumarin is significant; therefore, the two fluorescence signals can be separated using a spectrometer, allowing two receivers to simultaneously receive both fluorescence signals.

The application belongs to the technical field of pharmaceutical chemistry, and particularly relates to a coumarin derivative with anti-breast cancer activity and application thereof. The chemical structural formula of the coumarin derivative is shown in formula (I) or formula (II), wherein R1 is an electron-withdrawing or electron-donating group, and R2 is a saturated nitrogen heterocycle or an aromatic nitrogen heterocycle. The coumarin analog prepared by the application has a certain inhibitory effect on breast cancer cells.

The invention belongs to the field of pesticides, and particularly relates to a phenylamidine compound containing a coumarin structure or a pesticide acceptable salt, a composition and application of the phenylamidine compound, the compound has the structure shown in the formula (I), and all groups in the formula are defined in the specification. According to the compound disclosed by the invention, a substituted coumarin structure is introduced on the basis of an amidine structure, and the compound capable of being used as a bactericide is developed and obtained and has very outstanding biological activity and crop safety.

The invention discloses a preparation method and application of aerogel for detecting and adsorbing aluminum ions, and belongs to the technical field of sewage treatment. According to the method, a halloysite nanotube is taken as a matrix, and the HNT-KC probe with specific fluorescence response to aluminum ions is synthesized through silanization, acylating chlorination and gradual reaction with a coumarin derivative and furohydrazide; meanwhile, preparing aldehyde-terminated polyethylene glycol as a cross-linking agent; finally, the probe, a chitosan solution and an aldehyde-terminated polyethylene glycol solution are subjected to co-gelation through a Schiff base reaction, and the composite aerogel is prepared through dehydration aging and freeze drying. The aerogel integrates fluorescence detection and adsorption removal functions, has the advantages of high specific surface area, porous structure, high sensitivity, strong selectivity and good stability, can realize rapid detection and efficient removal of aluminum ions in a water body, is green in raw materials and simple in process, and has a wide application prospect in the field of water treatment.

The invention provides nucleic acid base composite nano-particles and a preparation method thereof. The preparation method comprises the following steps: taking PDMA and an acrylate adenine monomer as raw materials, carrying out polymerization to obtain a diblock polymer, taking the diblock polymer and a coumarin monomer as raw materials, carrying out polymerization to obtain PA, and assembling the PA into a nucleated cross-linked nano-micelle; pDMA and an acrylate thymine monomer are used as raw materials and polymerized to obtain a block polymer, and the block polymer is assembled into the nano-micelle; and mixing the core cross-linked nano-micelles with the nano-micelles, and interacting to obtain the composite nano-particles. According to the preparation method disclosed by the invention, the core-crosslinked nano-micelle containing adenine is constructed, and the nano-micelle has poly (N, N-dimethylacrylamide) with a hydrophilic block, and the poly (N, N-dimethylacrylamide) can stably exist in an aqueous solution; and mixing the core-crosslinked nano-micelles with the nano-micelles to obtain the composite nano-particles through biologically excited nuclear-base interaction.

This invention discloses a coumarin-linked conjugated porous polymer, its preparation method, and its applications. The preparation method includes: subjecting a homogeneous mixture containing o-hydroxybenzaldehyde monomer, phenylacetonitrile monomer, solvent, and catalyst to a Knauven-Gail condensation reaction to obtain the coumarin-linked conjugated porous polymer. The coumarin used in this invention to prepare the coumarin-linked conjugated porous polymer is a highly planar molecule. Its full conjugation extends the absorption wavelength to the visible light region and enhances π-electron excitation, thus exhibiting strong photosensitivity. The resulting series of coumarin-linked highly conjugated nanoarray conjugated porous polymers possess high porosity and a fully conjugated structure, making them highly efficient for applications in photocatalysis, fluorescence sensing, and optoelectronic devices.

The method of obtaining modified carbon quantum dots by N-substitution of compounds from the coumarin group, consists, according to the invention, in the fact that the carbon quantum dots, obtained by carbonisation of biomass constituting, inter alia, 1 biologically active substance having free carboxyl groups, are modified by the formation of an amide bond in an aqueous medium, under normal conditions, and then purified using dialysis membranes. During grafting, the ratio of dye to EDC is 1 to 1.1. The invention also relates to the carbon quantum dots with a fluorescence emission spectrum in the range 380- 800 nm, obtained by this method.

The application discloses a preparation method of a temperature-ultraviolet light double response type functional polymer, specifically comprising the following steps: preparing a macromolecular initiator PEG2-ABCPA; preparing a p-styrylmethyl diethanolamine borate monomer VMAB; and performing a free radical polymerization reaction on the macromolecular initiator PEG2-ABCPA, the p-styrylmethyl diethanolamine borate monomer VMAB and 7-(4-vinylbenzyloxy)-4-methyl coumarin VBC to obtain a block copolymer, i.e. the temperature-ultraviolet light double response type functional polymer. The polymer not only has unique amphiphilic properties, but also exhibits double response to temperature and ultraviolet light, thereby showing wide application prospects in the fields of drug controlled release, intelligent materials, photoelectric devices and the like.

The present invention provides novel water-soluble compositions comprising sesquiterpene coumarin enriched fractions containing different ratios of sesquiterpene coumarin derived from Ferula species for the treatment of Parkinson's disease via inhibition of MAOenzyme (MAO-A and MAO-B). The invention also provides a method of preparation of ethyl-acetate extract and sesquiterpene coumarin enriched fractions from asafoetida (Hing) that is oleo-gum-resin of Ferula assafoetida using silica gel column chromatography wherein the mobile phase consists of differing compositions of hexane, toluene, chloroform,ethyl-acetate, and methanol. The invention provides the method to prepare the formulation using a multistep process comprising of preparing sesquiterpene coumarin enriched fraction followed by preparing water-soluble formulation. The final composition improves the water solubility, imparts long-term stability, exerts MAO inhibitory activity, and improves pharmaceutical compliance.

The application discloses a kind of chiral dihydrofuranocoumarin compounds, its synthesis method and application, belong to organic chemistry synthesis and medical field;The structure of the compound is with C3 position amino methyl substitution chiral center dihydrofuranocoumarin as skeleton, its synthesis method is: at room temperature, copper salt and chiral ligand (L) are dissolved in organic solvent and stirred, then 4-ethynyl cyclic carbamic acid ester (I), 4-hydroxycoumarin (II) are sequentially added, after stirring reaction is completed, direct separation and purification are obtained;The compound provided by the application can be efficiently converted into other novel furanocoumarin compounds by simple reaction, and has good potential application value in antitumor drug research;The synthesis method of the application has the advantages of novelty, simple operation, mild reaction condition, good substrate universality, high yield, high stereoselectivity and the like.

In various embodiments compounds that induce and / or promote autophagy are provided as wells as methods of use thereof (e.g., in the prophylaxis or treatment of chronic pathologies associated with aging). In one illustrative, but non-limiting embodiments the compound(s) comprise, inter alia, coumarin 106.

The invention discloses a preparation method and application of a coumarin-hydrazide-based semi-sandwich type iridium and ruthenium complex, the structural formula of the complex is as shown in formula (I), the complex takes a coumarin-hydrazide derivative as an NO bidentate ligand, and an oxygen coordination atom in the ligand is derived from hydroxyl of a coumarin mother nucleus; iridium or ruthenium is used as a metal center, benzene is used as a unique solvent, alkali does not need to be added additionally under the protection of nitrogen, and the ligand can be prepared through a coordination reaction of the ligand and a metal dimer. The complex shows good anti-cancer activity, the drug effect of the complex is close to that of cis-platinum, and the complex has the potential value of becoming a novel anti-cancer drug.

The preparation method of the 3-phenylcoumarin derivative comprises the following steps: mixing 2-hydroxybenzaldehyde, a benzyl halide compound, a carbonyl metal compound, palladium acetate, 4, 5-bis (diphenylphosphine)-9, 9-dimethyl xanthene, sodium carbonate and dimethyl sulfoxide, stirring and reacting at 70-90 DEG C for 13-15 hours, filtering, washing and drying to obtain the 3-phenylcoumarin derivative. And then carrying out post-treatment on the reaction product to obtain the 3-phenylcoumarin derivative. The solid carbonyl metal compound is adopted as a carbonyl source, and a specific catalyst system and mild reaction conditions are combined, so that the 3-phenylcoumarin derivative can be efficiently, safely and easily synthesized, and the method has a relatively good application prospect.

This invention discloses a palladium-catalyzed asymmetric hydrogenation method for synthesizing chiral dihydrocoumarin from tetrasubstituted olefins. The method uses a chiral bisphosphine complex of palladium as a catalyst and a tetrasubstituted olefin as a substrate to synthesize chiral dihydrocoumarin via asymmetric hydrogenation. This invention employs a homogeneous palladium catalytic system to achieve asymmetric hydrogenation of tetrasubstituted olefins with high yield, high enantioselectivity (up to 99% enantiomeric excess), and high diastereoselectivity (diastereomer ratio >20:1), producing chiral dihydrocoumarin. The method is simple and practical, the catalyst is commercially available, the reaction conditions are mild, energy consumption is low, and it is environmentally friendly.

The invention discloses an application of coumarin substances in prevention and treatment of eucalyptus bacterial wilt. The coumarin substances are daphnetin and 6-methyl coumarin. Bacteriostatic experiment research results show that the daphnetin and the 6-methylcoumarin have an obvious inhibition effect on growth and proliferation of eucalyptus bacterial wilt bacteria when being used independently or used in combination. When the daphnetin is independently used, the effective concentration of the daphnetin is greater than or equal to 62.5 mu g / mL when the daphnetin has an obvious inhibition effect on eucalyptus bacterial wilt pathogenic bacteria; when the 6-methylcoumarin is singly used, the effective concentration of the 6-methylcoumarin is greater than or equal to 125 mu g / mL when the 6-methylcoumarin has an obvious inhibition effect on eucalyptus bacterial When the daphnetin and the 6-methylcoumarin are used in a combined manner, the effective concentration of the daphnetin and the effective concentration of the 6-methylcoumarin are both larger than or equal to 31.25 microgram / mL when the daphnetin and the 6-methylcoumarin show an obvious inhibition effect on eucalyptus bacterial wilt pathogenic bacteria, and therefore the daphnetin and the 6-methylcoumarin can serve as bacteriostatic agents to be used for preventing and treating eucalyptus bacterial wilt diseases and have potential application prospects of biocontrol preparations.

Invisible fluorescent solvent inkjet ink compositions comprising a fluorescent colorant comprising a coumarin, a solvent, a binder resin, and a conductive agent are described.

The present invention provides a medicament for the treatment or prevention of a cell proliferative disorder, the medicament comprising as an active ingredient a compound represented by formula (I):or a pharmaceutically acceptable salt thereof, wherein the medicament is used in such a manner that:(a) said compound or salt is administered twice weekly for 3 weeks,(b) administration of said compound or salt is paused for the following 1 week, and(c) steps (a) and (b) are subsequently repeated at least once.

The application discloses a coumarin tyramine glycoside compound and application thereof in preparation of PTP1B inhibitors and anti-diabetic drugs, and a structural formula of the compound is shown in the following formula: wherein R represents a fatty (aromatic) acyl or a monosaccharide substituent, and R' represents a hydrogen atom or an acetyl group; the compound is prepared by taking 4-methoxycoumarin tyramine 4'-acetyl-O-L-rhamnose as a starting material, introducing a fatty (aromatic) acyl or a monosaccharide substituent into a 2-position hydroxyl and a 3-position hydroxyl of L-rhamnose at the same time, and then removing a protecting group of a sugar group. Pharmacological activity tests show that the compound has good inhibitory activity on protein tyrosine phosphatase 1B, and it is indicated that the coumarin tyramine glycoside compound can be researched and utilized as a new type of PTP1B inhibitor and anti-diabetic drug.

This invention relates to the field of photoelectric-fluorescence dual-mode synergistic sensing technology, and particularly to a hydrazine hydrate photoelectric-fluorescence dual-mode sensor based on pyridine-coumarin-sensitized zinc oxide, its preparation method, and its application. The sensor includes a sensing electrode module, which comprises an electrode substrate on which a ZnO@NPC composite material is modified. The ZnO@NPC composite material consists of a zinc oxide nanorod array layer, a (3-bromopropyl)trimethoxysilane coupling layer, and a pyridine-coumarin sensitization layer sequentially modified on the electrode substrate surface. The pyridine-coumarin sensitization layer modification amount is 0.1~1.0 μmol·cm⁻¹. 2 The mass ratio of the sensor to the zinc oxide nanorod array layer is 3:1. This dual-mode sensor exhibits advantages such as high sensitivity to hydrazine hydrate, excellent dual-mode signal synergy, and simple preparation, solving the problems of "complex preparation, low detection sensitivity, and poor dual-mode signal synergy" in existing hydrazine hydrate detection technologies.

The present application relates to the technical field of detection, and discloses a piperazine coumarin derivative, a synthesis method and application thereof. The piperazine coumarin derivative provided by the present application takes coumarin as a matrix, can be used for double-channel double-emission area distinguishing detection of hydrazine and sulfur dioxide, and has high selectivity, high sensitivity and excellent stability. The piperazine coumarin derivative provided by the present application can detect hydrazine (N2H4) and sulfur dioxide (SO2) at a cell level; also has the ability of rapidly detecting N2H4 in different soil samples and test papers, and has great potential in the analysis and monitoring of environmental pollutants.

The application discloses a method for extracting coumarin from Cnidium monnieri and application thereof. The method comprises the following steps: taking dried Cnidium monnieri as raw material, crushing the raw material to obtain coarse powder, and performing defatting treatment to obtain defatted coarse powder; adding the defatted coarse powder into 85-95% (volume concentration) ethanol aqueous solution, performing ball milling, microwave extraction, filtering to obtain filtrate I, and concentrating to obtain thick paste; then adding the thick paste into mixed solvent A, performing ultrasonic extraction under heating, filtering to obtain extraction liquid; finally, adding the extraction liquid into mixed solvent B, performing extraction, separating to obtain organic phase and aqueous phase, reducing and concentrating the organic phase to obtain solid I, adjusting the pH of the aqueous phase to 7.5-8, filtering to obtain filtrate II, adding hydrochloric acid solution into the filtrate II under stirring until the pH is 2-3, performing cold storage, and performing suction filtration to obtain solid II; and combining the solid I and the solid II, and drying to obtain the product. The method is simple, the total coumarin extraction rate is high, and the content is high. The method can be used for preparation of coumarin drugs, and has good market promotion value.

The application discloses a [2.2] cyclophane coumarin fluorescent probe, a preparation method and a method for detecting chiral molecules, and the fluorescent probe comprises a fluorescent probe structure, and the fluorescent probe structure is shown as formula (I). The [2.2] cyclophane skeleton is introduced into the coumarin molecule, and the fluorescent probe can be applied to fluorescence recognition of chiral molecules. The fluorescent probe can chemoselectively and enantioselectively recognize histidine in a mixed solution of tetrahydrofuran and HEPES aqueous solution, and can quantitatively detect the concentration of histidine and the ee value of histidine. The probe is an achiral fluorescent probe, can reduce the difficulty of separation of chiral compounds, greatly reduces the synthesis cost of the fluorescent probe, and opens up a new amino acid recognition probe.

The invention discloses a preparation method and application of a semi-sandwich type iridium complex containing a tridentate ligand. The structural formula of the complex is shown as a formula (I), a ligand and a dimer react under the reaction condition, sodium bicarbonate is continuously added into a reaction system, proton H on coumarin unit carbon in the ligand can be selectively removed, and therefore the semi-sandwich type iridium complex (I) of the CNS tridentate ligand is obtained. Wherein a coordination carbon atom (C, blue) in the tridentate chelate ligand is derived from a coumarin unit, a nitrogen atom (N, red) is derived from a Schiff base structure, a sulfur atom (S, pink) is derived from thiosemicarbazide, the obtained iridium complex shows good anticancer activity, the performance of the iridium complex is close to or even superior to that of commercial cis-platinum, and the iridium complex is a very potential anticancer drug.

The invention relates to a quick detection method of pyrethroid, belongs to the technical field of fluorescent probes, and discloses a plurality of micromolecular fluorescent probes based on coumarin derivatives as well as a preparation method and application of the micromolecular fluorescent probes. The invention provides a plurality of micromolecular fluorescent probes DAC, DBC, DCC, DDC, DEC, DFC, DGC, DHC and DIC for targeted detection of pyrethroid pesticides based on coumarin derivatives, when the probes act with the pyrethroid pesticides, the fluorescence becomes strong from weak, and the fluorescence intensity is significantly enhanced in a certain range. Fluorescence performance tests show that the coumarin derivative-based micromolecular fluorescent probe pyrethroid pesticide has good linear response, and has good stability and strong anti-interference capability under different pH, temperature, salt concentration and illumination conditions. According to the actual sample detection principle, the pyrethroid pesticide in crops is detected, the detection limit is as low as 9.7 pM, the adding standard recovery rate is 99%-102%, and the results are consistent with those of a liquid chromatography method and an on-site rapid detection method. Therefore, the fluorescent probe provided by the invention has a good application prospect.