101 results about "Coumarin" patented technology

This invention discloses a strain of *Neocallimastix* LGM-ZA9 and its application in the preparation of a coumarin-degrading feed additive. *Neocallimastix* LGM-ZA9 is classified as *Neocallimastix* sp., with accession number CGMCC No. 17592, and its optimal growth temperature is 39℃. The fermentation broth of *Neocallimastix* LGM-ZA9 can be used to degrade the anti-nutritional factor coumarin. The method is simple: the coumarin-containing material to be degraded is mixed with the fermentation broth, sterilized, and then the fermentation broth is added for anaerobic fermentation to degrade the coumarin. This preparation method is simple, highly operable, and can significantly reduce the coumarin content, providing a new method for feeding ruminants with sweet clover as a high-quality forage.

The invention discloses an ultraviolet-controlled photo-reversible dynamic crosslinking high polymer material with reversible antibacterial, shape deformation and toughening functions, and a preparation method thereof, belonging to the technical field of high polymer materials. Comprising the following steps: step 1) dissolving coumarin-3-formic acid, three-arm PCL (polycaprolactone), dicyclohexylcarbodiimide and 4-dimethylaminopyridine in dry dichloromethane in an inert atmosphere to obtain a reaction mixture; the invention relates to a preparation method of a coumarin-terminated three-arm polycaprolactone cross-linked polymer material.The preparation method comprises the following steps of (1) preparing coumarin-terminated three-arm polycaprolactone, (2) heating a reaction mixture obtained in the step (1) for set time, and filtering and separating an obtained product to obtain coumarin-terminated three-arm polycaprolactone, and (3) cross-linking and curing for set time through 365 nm ultraviolet to obtain a target product cross-linked polymer material.The function is highly integrated and dynamically adjustable, and the limitation that a traditional material is single in function is broken through.

Provided are a pyrrolo[h]coumarin dye and a use thereof in sequencing. Specifically provided are a compound represented by formula I or a stereoisomer, tautomer, crystal form, salt, ester or solvate thereof. The compound has the advantages of high brightness and high stability. The compound exhibits strong fluorescence under blue light excitation. When used for labeling dNTPs and then applied in gene sequencing, the sequencing signal, resolution and throughput are all significantly improved, and the sequencing quality is significantly improved.

The invention discloses a preparation method of a 3-substituted isocoumarin compound, and belongs to the technical field of coumarin compound synthesizing.The preparation method comprises the steps that a 2-bromophenylboronic acid compound and alkyne serve as reaction substrates, a bidentate nitrogen ligand is added and dissolved in a solvent, under the action of alkali and a copper catalyst, a normal-pressure one-step cyclization reaction is conducted in the CO2 atmosphere, and the 3-substituted isocoumarin compound is obtained. The 3-substituted isocoumarin compound is obtained. According to the method, CO2, alkyne and 2-bromophenylboronic acid are used as reaction raw materials, cheap copper is used as a catalyst, the use amount is small, the 3-substituted isocoumarin is obtained through carbon-carbon coupling-cyclization by a CO2 activation one-step method under normal pressure, the conditions are mild, the cost is low, and the method is environmentally friendly.

This invention discloses a coumarin-linked conjugated porous polymer, its preparation method, and its applications. The preparation method includes: subjecting a homogeneous mixture containing o-hydroxybenzaldehyde monomer, phenylacetonitrile monomer, solvent, and catalyst to a Knauven-Gail condensation reaction to obtain the coumarin-linked conjugated porous polymer. The coumarin used in this invention to prepare the coumarin-linked conjugated porous polymer is a highly planar molecule. Its full conjugation extends the absorption wavelength to the visible light region and enhances π-electron excitation, thus exhibiting strong photosensitivity. The resulting series of coumarin-linked highly conjugated nanoarray conjugated porous polymers possess high porosity and a fully conjugated structure, making them highly efficient for applications in photocatalysis, fluorescence sensing, and optoelectronic devices.

The application discloses a preparation method of a temperature-ultraviolet light double response type functional polymer, specifically comprising the following steps: preparing a macromolecular initiator PEG2-ABCPA; preparing a p-styrylmethyl diethanolamine borate monomer VMAB; and performing a free radical polymerization reaction on the macromolecular initiator PEG2-ABCPA, the p-styrylmethyl diethanolamine borate monomer VMAB and 7-(4-vinylbenzyloxy)-4-methyl coumarin VBC to obtain a block copolymer, i.e. the temperature-ultraviolet light double response type functional polymer. The polymer not only has unique amphiphilic properties, but also exhibits double response to temperature and ultraviolet light, thereby showing wide application prospects in the fields of drug controlled release, intelligent materials, photoelectric devices and the like.

The present invention provides novel water-soluble compositions comprising sesquiterpene coumarin enriched fractions containing different ratios of sesquiterpene coumarin derived from Ferula species for the treatment of Parkinson's disease via inhibition of MAOenzyme (MAO-A and MAO-B). The invention also provides a method of preparation of ethyl-acetate extract and sesquiterpene coumarin enriched fractions from asafoetida (Hing) that is oleo-gum-resin of Ferula assafoetida using silica gel column chromatography wherein the mobile phase consists of differing compositions of hexane, toluene, chloroform,ethyl-acetate, and methanol. The invention provides the method to prepare the formulation using a multistep process comprising of preparing sesquiterpene coumarin enriched fraction followed by preparing water-soluble formulation. The final composition improves the water solubility, imparts long-term stability, exerts MAO inhibitory activity, and improves pharmaceutical compliance.

In various embodiments compounds that induce and / or promote autophagy are provided as wells as methods of use thereof (e.g., in the prophylaxis or treatment of chronic pathologies associated with aging). In one illustrative, but non-limiting embodiments the compound(s) comprise, inter alia, coumarin 106.

The invention discloses a preparation method and application of a coumarin-hydrazide-based semi-sandwich type iridium and ruthenium complex, the structural formula of the complex is as shown in formula (I), the complex takes a coumarin-hydrazide derivative as an NO bidentate ligand, and an oxygen coordination atom in the ligand is derived from hydroxyl of a coumarin mother nucleus; iridium or ruthenium is used as a metal center, benzene is used as a unique solvent, alkali does not need to be added additionally under the protection of nitrogen, and the ligand can be prepared through a coordination reaction of the ligand and a metal dimer. The complex shows good anti-cancer activity, the drug effect of the complex is close to that of cis-platinum, and the complex has the potential value of becoming a novel anti-cancer drug.

This invention discloses a palladium-catalyzed asymmetric hydrogenation method for synthesizing chiral dihydrocoumarin from tetrasubstituted olefins. The method uses a chiral bisphosphine complex of palladium as a catalyst and a tetrasubstituted olefin as a substrate to synthesize chiral dihydrocoumarin via asymmetric hydrogenation. This invention employs a homogeneous palladium catalytic system to achieve asymmetric hydrogenation of tetrasubstituted olefins with high yield, high enantioselectivity (up to 99% enantiomeric excess), and high diastereoselectivity (diastereomer ratio >20:1), producing chiral dihydrocoumarin. The method is simple and practical, the catalyst is commercially available, the reaction conditions are mild, energy consumption is low, and it is environmentally friendly.

The invention discloses an application of coumarin substances in prevention and treatment of eucalyptus bacterial wilt. The coumarin substances are daphnetin and 6-methyl coumarin. Bacteriostatic experiment research results show that the daphnetin and the 6-methylcoumarin have an obvious inhibition effect on growth and proliferation of eucalyptus bacterial wilt bacteria when being used independently or used in combination. When the daphnetin is independently used, the effective concentration of the daphnetin is greater than or equal to 62.5 mu g / mL when the daphnetin has an obvious inhibition effect on eucalyptus bacterial wilt pathogenic bacteria; when the 6-methylcoumarin is singly used, the effective concentration of the 6-methylcoumarin is greater than or equal to 125 mu g / mL when the 6-methylcoumarin has an obvious inhibition effect on eucalyptus bacterial When the daphnetin and the 6-methylcoumarin are used in a combined manner, the effective concentration of the daphnetin and the effective concentration of the 6-methylcoumarin are both larger than or equal to 31.25 microgram / mL when the daphnetin and the 6-methylcoumarin show an obvious inhibition effect on eucalyptus bacterial wilt pathogenic bacteria, and therefore the daphnetin and the 6-methylcoumarin can serve as bacteriostatic agents to be used for preventing and treating eucalyptus bacterial wilt diseases and have potential application prospects of biocontrol preparations.

This invention relates to the field of photoelectric-fluorescence dual-mode synergistic sensing technology, and particularly to a hydrazine hydrate photoelectric-fluorescence dual-mode sensor based on pyridine-coumarin-sensitized zinc oxide, its preparation method, and its application. The sensor includes a sensing electrode module, which comprises an electrode substrate on which a ZnO@NPC composite material is modified. The ZnO@NPC composite material consists of a zinc oxide nanorod array layer, a (3-bromopropyl)trimethoxysilane coupling layer, and a pyridine-coumarin sensitization layer sequentially modified on the electrode substrate surface. The pyridine-coumarin sensitization layer modification amount is 0.1~1.0 μmol·cm⁻¹. 2 The mass ratio of the sensor to the zinc oxide nanorod array layer is 3:1. This dual-mode sensor exhibits advantages such as high sensitivity to hydrazine hydrate, excellent dual-mode signal synergy, and simple preparation, solving the problems of "complex preparation, low detection sensitivity, and poor dual-mode signal synergy" in existing hydrazine hydrate detection technologies.

The application discloses a method for extracting coumarin from Cnidium monnieri and application thereof. The method comprises the following steps: taking dried Cnidium monnieri as raw material, crushing the raw material to obtain coarse powder, and performing defatting treatment to obtain defatted coarse powder; adding the defatted coarse powder into 85-95% (volume concentration) ethanol aqueous solution, performing ball milling, microwave extraction, filtering to obtain filtrate I, and concentrating to obtain thick paste; then adding the thick paste into mixed solvent A, performing ultrasonic extraction under heating, filtering to obtain extraction liquid; finally, adding the extraction liquid into mixed solvent B, performing extraction, separating to obtain organic phase and aqueous phase, reducing and concentrating the organic phase to obtain solid I, adjusting the pH of the aqueous phase to 7.5-8, filtering to obtain filtrate II, adding hydrochloric acid solution into the filtrate II under stirring until the pH is 2-3, performing cold storage, and performing suction filtration to obtain solid II; and combining the solid I and the solid II, and drying to obtain the product. The method is simple, the total coumarin extraction rate is high, and the content is high. The method can be used for preparation of coumarin drugs, and has good market promotion value.

The application discloses a [2.2] cyclophane coumarin fluorescent probe, a preparation method and a method for detecting chiral molecules, and the fluorescent probe comprises a fluorescent probe structure, and the fluorescent probe structure is shown as formula (I). The [2.2] cyclophane skeleton is introduced into the coumarin molecule, and the fluorescent probe can be applied to fluorescence recognition of chiral molecules. The fluorescent probe can chemoselectively and enantioselectively recognize histidine in a mixed solution of tetrahydrofuran and HEPES aqueous solution, and can quantitatively detect the concentration of histidine and the ee value of histidine. The probe is an achiral fluorescent probe, can reduce the difficulty of separation of chiral compounds, greatly reduces the synthesis cost of the fluorescent probe, and opens up a new amino acid recognition probe.

The invention relates to a quick detection method of pyrethroid, belongs to the technical field of fluorescent probes, and discloses a plurality of micromolecular fluorescent probes based on coumarin derivatives as well as a preparation method and application of the micromolecular fluorescent probes. The invention provides a plurality of micromolecular fluorescent probes DAC, DBC, DCC, DDC, DEC, DFC, DGC, DHC and DIC for targeted detection of pyrethroid pesticides based on coumarin derivatives, when the probes act with the pyrethroid pesticides, the fluorescence becomes strong from weak, and the fluorescence intensity is significantly enhanced in a certain range. Fluorescence performance tests show that the coumarin derivative-based micromolecular fluorescent probe pyrethroid pesticide has good linear response, and has good stability and strong anti-interference capability under different pH, temperature, salt concentration and illumination conditions. According to the actual sample detection principle, the pyrethroid pesticide in crops is detected, the detection limit is as low as 9.7 pM, the adding standard recovery rate is 99%-102%, and the results are consistent with those of a liquid chromatography method and an on-site rapid detection method. Therefore, the fluorescent probe provided by the invention has a good application prospect.

The application discloses 3-substituted coumarin derivatives 1a-14a and symmetrical biscoumarin derivatives 1b-25b and pharmaceutically acceptable salts, solvates or hydrates thereof. The compounds provided by the application are specific inhibitors of ATP citrate lyase (ACLY), and the half maximal inhibitory concentration (IC 50 ) of the former is 2-30 muM, and the IC 50 ) of the latter is 0.065-4 muM, so the compounds have potential therapeutic value for a plurality of diseases with abnormally high expression of ACLY, such as cancer, hyperlipidemia and arteriosclerotic cardiovascular diseases.

The present invention relates to a cyclic peptide comprising an active region comprising the amino acid sequence X1X2X3X4X5X6 or a salt, derivative, prodrug or mimetic thereof. X2 and X5 are arginine; and either:X1 is (7-methoxy-coumarin-4-yl)-Ala-OH (Dac) and X3, X4 and X6 are any amino acid; orX3 is sarcosine (Sar) and X1, X4 and X6 are any amino acid; orX4 is 5,5-dimethylproline (dmPro) or 3-amino-3-(2-naphthyl)propionic acid (Nap) and X1, X3 and X6 are any amino acid; orX6 is Nap and X1, X3 and X4 are any amino acid.The present invention further relates to a pharmaceutical composition comprising the cyclic peptide and the peptide for use in medicine, and particularly cancer.

The invention discloses a screening method and application of natural small molecules for inhibiting growth of Aspergillus carbonniger and synthesis of ochratoxin A, and relates to the technical field of bioinformatics. The invention particularly provides an application of coumarin or coumarin derivatives in preparation of a preparation for inhibiting growth of aspergillus carbonus niger and / or synthesis of ochratoxin A. The invention further provides a preparation method of the coumarin or the coumarin derivatives. According to the invention, SE of Aspergillus carbonus is taken as a target spot, small molecules with potential inhibitory activity are rapidly screened from a natural compound library by using a virtual screening technology, and experiments prove that coumarin and specific derivatives thereof not only can effectively inhibit the growth of Aspergillus carbonus, but also can efficiently inhibit the biosynthesis of OTA, so that the coumarin and specific derivatives thereof can be used for preparing drugs for inhibiting OTA. A theoretical basis and technical support are provided for the application of the strain in the postharvest preservation of agricultural products.

This invention discloses coumarin Schiff base derivatives. These derivatives are based on coumarin, and a series of coumarin derivatives with Schiff base structures are introduced through a reaction. These compounds exhibit good antifungal activity against *Botrytis cinerea*, *Alternaria alternata*, *Fusarium oxysporum*, and *Alternaria alternata*, opening up new avenues for the control of various plant fungal diseases and possessing broad development potential and promising application prospects. Furthermore, in vitro antioxidant experiments on these Schiff base compounds showed that they exhibit excellent scavenging abilities against DPPH radicals, ABTS radicals, and hydroxyl radicals, with antioxidant performance comparable to the positive control ascorbic acid, demonstrating their potential as novel antioxidants.

The invention belongs to the technical field of compounds, and provides a Schiff base bridged coumarin indole compound as well as a preparation method and application thereof. The coumarin indole compound bridged by the Schiff base or the pharmaceutically acceptable salt of the coumarin indole compound provided by the invention has a structure as shown in a formula I. In-vitro antimicrobial activity evaluation shows that the coumarin indole compound has a good inhibition effect on gram-positive bacteria and gram-negative bacteria, and particularly has a good antibacterial effect on staphylococcus aureus; compared with the prior art, the derivative disclosed by the invention has the advantages that the drug resistance is not easy to generate, so that the derivative or the pharmaceutically acceptable salt thereof can be used for preparing antibacterial active drugs, more efficient and safe candidate drug molecules are provided for clinical treatment, and the clinical treatment problems of increasingly serious drug resistance, stubborn germs, newly appearing harmful microorganisms and the like are favorably solved.

This invention relates to the fields of pesticide chemistry and medicinal chemistry, and in particular to a coumarin derivative containing a substituted phenyl ether structure, its preparation method, and its applications. This invention organically combines a coumarin structure with a substituted phenyl ether fragment to synthesize a novel class of coumarin derivatives containing a substituted phenyl ether structure. These compounds exhibit excellent antifungal activity and broad-spectrum activity, demonstrating superior inhibitory activity against *Agropyron cristatum*, *Sclerotinia sclerotiorum*, *Botrytis cinerea*, and *Tricholoma matsutake*. This invention provides a foundation for the development of novel fungicides using coumarin derivatives containing substituted phenyl ether structures as active ingredients.

The invention discloses a method for preparing a polyfluoroalkyl coumarin derivative, and belongs to the field of organic synthesis. According to the method, cheap and easily available polyfluorocarboxylic acid anhydride is used as a polyfluoroalkylating reagent, and in-situ activation of NHPI is combined to prepare the C3-site polyfluoroalkyl substituted coumarin derivative. Under the condition of visible light catalysis, polyfluoroalkyl carboxylic acid anhydride is used as a polyfluoroalkylating reagent, polyfluoroalkyl free radicals are generated through in-situ activation of NHPI, the free radicals and aromatic propiolate are subjected to a free radical cyclization rearrangement reaction, and finally preparation of the C3-site polyfluoroalkyl substituted coumarin derivative is achieved. The polyfluoroalkyl carboxylic acid anhydride used in the method is stable in property, low in price and easy to obtain, and the synthesis cost is reduced. The method is wide in applicable substrate range and applicable to various polyfluoroalkyl carboxylic acid anhydrides, and the tolerance of reaction functional groups is good.

The application provides a coumarin and carbazole dipyridine salt type water-soluble two-photon initiator, a preparation method and application thereof, and in particular, a use in the field of laser micro-nano processing technology. In the structural general formula of the two-photon initiator, R1 is selected from hydrogen, C 1‑12 alkyl, C 1‑12 hydroxyalkyl or an ethereal unit with a number of repetitions of 1-12; R2 is selected from methyl, ethyl or isopropyl; and Y is a corresponding anion. The two-photon initiator has a large conjugated structure, and the introduction of the dipyridine salt enables the two-photon initiator to be soluble in water. As an initiator, the two-photon initiator can initiate two-photon photopolymerization in an aqueous phase, and the two-photon absorption cross section is greater than 100 GM, so that the power threshold of two-photon photoinitiation during processing is low, i.e. 25 mW. Therefore, the high initiation activity and low two-photon polymerization threshold of the two-photon initiator enable the two-photon initiator to efficiently initiate two-photon polymerization in an aqueous phase, and the effect of processing three-dimensional hydrogels by using two-photon polymerization is outstanding.

The application discloses a perovskite solar cell, which comprises a conductive substrate, a hole transport layer, a perovskite light-absorbing layer, an electron transport layer and an electrode layer from bottom to top, wherein the perovskite light-absorbing layer is doped with glutamic acid coumarin derivative, and the structure is shown in the following formula. The application further discloses a preparation method of the perovskite solar cell. The perovskite light-absorbing layer is prepared by doping glutamic acid coumarin derivative in a perovskite precursor solution, which promotes the absorption of sunlight by the perovskite thin film, regulates the stress of the perovskite light-absorbing layer, improves the surface energy of the perovskite precursor, reduces the recombination of photo-generated carriers of the perovskite solar cell, and makes the prepared perovskite solar cell have excellent photoelectric conversion efficiency and stability, and has a wide application prospect.

This invention provides a coumarin-chalcone hybrid compound, its preparation method, and its applications. The structure of the coumarin-chalcone hybrid compound is shown in Formula I. The coumarin-chalcone hybrid compound provided by this invention has a simple and easy synthesis process, readily available raw materials, and can significantly activate the transcriptional activity of Nrf2 at both the cellular and animal levels. It exhibits high safety, good cell membrane permeability, and can be taken orally. After oral administration, it primarily results in high exposure in the liver and can effectively alleviate liver damage caused by acetaminophen and alcohol.

The invention discloses an application of coumarin in preventing and treating bacterial wilt of cigars. The coumarin is applied to prevention and treatment of bacterial wilt of cigars. According to transcriptome and metabonomics data in the early stage, the inventor finds a stable and high-content organic compound coumarin in a bacterial wilt resistant cigar variety, cigars are used as an application crop, the high-incidence disease of cigars, namely bacterial wilt, is used as an application object, coumarin with different concentrations is investigated in detail, and the application amount of the coumarin is greatly increased. The prevention and treatment effect on the bacterial wilt of cigars is achieved by means of foliage spraying and root irrigation. Research finds that 15 mL of coumarin solution with the concentration of 20-80 mg / L needs to be applied to each cigar seedling, and the cigar bacterial wilt prevention and treatment effect is excellent.

The utility model provides a stirring device for coumarin, which comprises a stirring kettle, a top cover is detachably arranged at the top of the stirring kettle, a stirring shaft is rotatably arranged in the stirring kettle, a stirring blade is arranged at the side end of the stirring shaft, and a connecting cylinder sleeved on the outer side of the stirring shaft is rotatably arranged on the top cover. A scraping plate for scraping and cleaning the scraping plate adhered to the inner side wall of the stirring kettle is arranged on the connecting cylinder through a cross rod. According to the stirring device for the coumarin, disclosed by the utility model, not only can the basic requirement of stirring the coumarin be met, but also the coumarin adhered to the inner wall of the device can be automatically scraped and cleaned, so that the use requirement of people is fully met.

The application provides a molecule imprinting biochar for targeted adsorption of aflatoxin B1 and a preparation method and application thereof, and belongs to the technical field of functional biochar.The application takes oil tea pomace residue after solvent extraction as raw material, first prepares biochar COBC through pyrolysis, then obtains magnetic biochar F-COBC through magnetic modification, then obtains SF-COBC through SiO2 coating, finally takes SF-COBC as a carrier, 5,7-dimethyl coumarin as an aflatoxin B1 simulation template molecule, and performs polymerization reaction on the surface of SF-COBC with the aid of a functional monomer, a crosslinking agent and an initiator, elutes the template molecule, and obtains the molecule imprinting biochar.The molecule imprinting biochar has specific recognition sites for aflatoxin B1, has excellent targeted adsorption performance, and the adsorption amount of the molecule imprinting biochar for aflatoxin B1 can reach 200.02 ng / mg in 80 min, the molecule imprinting biochar is convenient to recover, the adsorption rate can still reach 76% after 5 cycles, and the molecule imprinting biochar can be effectively applied to the adsorption and removal of aflatoxin B1 in agricultural and forestry products, thereby providing a new scheme for the treatment of fungal toxins in the field of food safety.

The present application belongs to the technical field of preparation of viscosity-reducing glue, and particularly relates to a bio-based light-regulated detachable viscosity-reducing glue and a preparation method thereof. A coumarin chain extender is prepared through a reversible light cyclization reaction of a coumarin monomer, and then a light-regulated detachable viscosity-reducing glue base glue is synthesized through a chain extension reaction of the coumarin chain extender and a prepolymer synthesized from a polyol and an isocyanate. Then, reinforcing fillers and a silane coupling agent are added to the system to uniformly disperse the reinforcing fillers in the light-regulated detachable viscosity-reducing glue, so as to guarantee the mechanical properties such as tensile strength, peel strength and hardness of the light-regulated detachable viscosity-reducing glue. The light-regulated detachable viscosity-reducing glue can achieve the purpose of being detachable through irradiation of ultraviolet light of a fixed wave band, and has the advantage of controllable bonding strength. The light-regulated detachable viscosity-reducing glue not only has the performance of being light-regulated and detachable, but also is prepared from bio-based raw materials, which is conducive to the sustainable development of the ecological environment, and is mainly suitable for wafer manufacturing and processing and chip manufacturing industries.

The application provides a preparation method and application of a coumarin derivative labeled nucleoside triphosphate, and the preparation method comprises the following steps: (1) mixing compound AA-dUTP, compound Boc-6-Ahx-Osu and an alkali to react in the dark to obtain compound 1; (2) mixing compound 1 and an acid solution, and then mixing with an alkali solution to obtain compound 2; (3) mixing compound 2 and compound DEAC-NHS to react in the dark to obtain compound DEAC-12-dUTP, namely the coumarin derivative labeled nucleoside triphosphate. The preparation method provided by the application can efficiently synthesize the target product, and has the advantage of high synthesis yield.