61 results about "Canker" patented technology

The invention discloses application of polypeptide compounds in prevention and treatment of pathogenic microorganisms. The compound has a synergistic effect on agricultural fungi such as rhizoctonia solani, Sclerotinia sclerotiorum, Botrytis cinerea, Fusarium graminearum, Pyricaria oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae, pyricularia oryzae and pyricularia oryzae. The method comprises the following steps: carrying out sterilization on plant bacteria such as Xanthomonas oryzae PXO99A, Xanthomonas axonophora dispsp.citrijx-6, Pseudomonas solanacearum (Smith) BNCC335855, Pseudomonas sp. The method is characterized by comprising the following steps of: killing Aspergillus flavus ACCC32601 (Aspergillus flavus), Penicillium expansum ACCC 30898 (Penicillium expansum. EXPANSum ACCC 30898), Monilinia coerulea ACCC 36263 (Aspergillus oryzae, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger, Aspergillus niger and Aspergillus niger); the compound disclosed by the invention has a certain inhibition activity on various diseases caused by streptococcus agalactiae ATCC 27956, streptococcus dysgalactiae ATCC27957, Escherichia coli ATCC 25922 and Staphylococcus aureus, particularly, the compound Thiostreptin has a particularly remarkable inhibition effect on agricultural pathogenic bacteria, food-borne germs and cow mastitis germs, and is expected to be developed into a novel antibacterial drug, so that the compound disclosed by the invention can be used for preparing the antibacterial agent for preventing and treating various diseases caused by the streptococcus agalactiae ATCC 27956, the streptococcus dysgalactiae ATCC27957, the Escherichia coli ATCC 25922 and the Staphylococcus aureus.

The invention discloses an African trichoderma harzianum strain 6-5 and application thereof, the disclosed strain is named as Trichoderma harzianum, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No. 41930. The bacterial strain has obvious bacteriostatic activity on crop pathogenic bacteria such as colletotrichum capsici, apple cyst, botrytis cinerea, alternaria longibrachiata and phytophthora capsici, and fermentation liquor of the bacterial strain has good prevention and control effects on apple anthracnose leaf blight, apple bitter rot and apple tree canker.

The invention discloses bacillus atrophaeus YL84, a microbial agent and application, and belongs to the technical field of microorganisms. The strain is preserved in the China Center for Type Culture Collection on February 20, 2025, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 2025267. Sterile fermentation filtrate of the strain YL84 can effectively inhibit germination of conidia and growth of mycelia of valsa ceratosperma of Korla pear trees, can play a good role in preventing in-vitro branches and potted pear seedlings, in addition, the strain can secrete protease and cellulase and synthesize siderophores, has the capacity of dissolving inorganic phosphorus and producing IAA at the same time, and has a good application prospect. The strain has a remarkable antagonistic effect on five common plant pathogenic fungi in the Xinjiang Uygur autonomous region and is wide in antibacterial spectrum.

The invention belongs to the field of microorganisms, and particularly relates to pseudomonas chlororaphis D1 and application thereof in prevention and treatment of Korla pear tree canker. The pseudomonas chlororaphis strain D1 which has a good antagonistic effect on the Korla pear tree rot disease is separated from Korla pear tree barks, the strain has a good application prospect in the aspect of biological prevention and control of the Korla pear tree rot disease, and a new biocontrol bacterium resource is provided for prevention and control of the Korla pear tree rot disease.

The invention belongs to the field of agriculture and plant quarantine, and particularly relates to a method for detecting phomopsis helianthi based on RPA / CRISPR-Cas12a, which comprises the following steps: S1, designing a detection composition: designing a composition for detecting phomopsis helianthi, the composition comprising a specific primer RPA-F / R nucleic acid sequence, a specific crRNA nucleic acid sequence and probes FL-ssDNA and LF-ssDNA; s2, extracting DNA: extracting the genome DNA of the diseased tissue of the sunflower to be detected; s3, DNA isothermal amplification: taking the genome DNA obtained in the step S1 as a template, adding a specific primer RPA-F1 / R1 into the template, and carrying out RPA amplification to obtain an amplification product; s4, CRISPR / Cas12a reaction: the amplification product obtained in the S2 and the crRNA are respectively mixed with FL-ssDNA and LF-ssDNA probes for a cleavage reaction, and a reaction product FL and a reaction product LF are obtained; and S5, visual detection: respectively placing the reaction product FL and the reaction product LF in a fluorescent PCR instrument and on a Cas12 special detection test strip for detection. The method is based on an RPA / CRISPR-Cas12a technology, by designing a specific primer and a crRNA nucleic acid sequence, the detection duration of the phomopsis helianthi is greatly shortened, the detection sensitivity is improved, and the method has very high use value in detection scenes such as customs and the like.

The invention discloses a biocontrol Chryseobacterium sp. With'contact sterilization 'activity. The PcE1 is a biocontrol bacterium which is separated from rhizosphere soil of Chinese cabbage by utilizing a germ enrichment method and can inhibit the growth of various pathogenic bacteria in a cell contact manner. The genome of the PcE1 encodes a type VI secretion system (T6SS) gene cluster and a plurality of T6SS effect proteins with antibacterial activity. The biocontrol effect determination on plants shows that the PcE1 can inhibit the infection of pectobacterium carotovorum on carrots and also can inhibit the infection of citrus canker pathogens on citrus.

The invention relates to a method for cultivating butter crabs and a method for screening key genes formed by the butter crabs. The method comprises the following steps: a, screening blue crabs cultivated by the butter crabs; b, performing cultivation management; according to the breeding method, blue crabs are independently bred in a crab breeding device, and the crab breeding device comprises a breeding basket body; the sandy layer is laid at the bottom of the breeding basket body; the blowdown device is arranged in the breeding basket body, and water in the sandy layer, excrement and residual feed can be discharged by means of water level pressure generated by the inner side and the outer side of a blowdown chamber. By regulating and controlling the temperature and salinity of seawater, the proportion of the butter crabs in the blue crab breeding process is increased; the pollution discharge device is arranged in the crab breeding device, self-cleaning of the sandy layer is achieved, the stress reaction of crabs is reduced, meanwhile, the self-cleaning sandy layer can allow the crabs to dive in sand, the living state of the crabs in the nature is simulated, meanwhile, the crabs can be prevented from ulcer, and then the breeding success rate of the butter crabs is increased.

The present invention discloses the application of coumarin-3-carboxylic acid in preventing and treating plant bacterial diseases. The coumarin-3-carboxylic acid provided by the present invention has a significant inhibitory effect on plant bacterial diseases. The plant bacterial diseases are oryzae bacterial leaf blight, oryzae bacterial leaf streak, citrus canker, cantaloupe bacterial fruit spot, tomato bacterial wilt, strawberry bacterial angular leaf spot, cabbage black rot, cabbage soft rot, potato soft rot, mango bacterial angular leaf spot, cassava bacterial wilt, cucumber bacterial angular leaf spot, kiwifruit canker and banana bacterial soft rot. The coumarin-3-carboxylic acid provided by the present invention has protective and therapeutic effects on melon bacterial fruit spot.

This invention discloses a class of thiadiazole-benzohexacyclic compounds, their preparation methods, and applications. The structural formula is shown in Formula I. This invention introduces disulfide bond fragments into the thiadiazole-benzohexacyclic skeleton to obtain a novel class of thiadiazole-benzohexacyclic compounds. These compounds have a novel structure, a simple preparation method, readily available raw materials, and are unlikely to produce harmful byproducts. The compounds exhibit inhibitory effects against various plant pathogenic bacteria, particularly against *Citrus canker*, *Ralstonia solanacearum*, and *Bacterial angular leaf spot* of cucumber, demonstrating significant inhibitory effects. They can be used as agricultural bactericides and have great application value.

The invention discloses an application of trichoderma harzianum in biological prevention and treatment of wild apple tree canker, the classification name of the trichoderma harzianum is Bacillus velezensis Rifai, the preservation unit is China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO. 42231, and the preservation time is September 29, 2025. Experimental results show that the trichoderma harzianum has remarkable antagonistic activity on parasitic cyst, apple cyst, nutshell cyst, cyst hormaechei, prunus cyst and pear cyst, is environment-friendly and pollution-free, and also provides a safe and reliable new way for preventing and treating the valsa mali of the wild apples; in addition, the research also finds that the fermentation product of the trichoderma harzianum has a certain biocontrol effect on the in-vitro branches infected by the physosporium piricola, so that the trichoderma harzianum and the fermentation product of the trichoderma harzianum provided by the invention can be used for preventing and treating the wild apple tree canker, and have good development and application prospects.

This invention relates to the field of medicinal chemistry, specifically to a capsaicin-like compound containing isopropanolamine, its preparation method, and its application. This type of compound has a structure as shown in general formula (I). The invention uses capsaicin and isopropanolamine as pharmacophores, connecting the two pharmacophores with different bridging chains to synthesize a series of capsaicin-like derivatives containing isopropanolamine. These compounds exhibit excellent inhibitory effects on plant pathogenic bacteria such as rice bacterial blight, citrus canker, rice bacterial leaf streak, and kiwifruit canker; and plant pathogenic fungi such as cucumber gray mold, pepper wilt, rapeseed sclerotinia rot, eggplant verticillium wilt, wheat scab, potato late blight, blueberry root rot, grape coccidioidomyces, dragon fruit anthracnose, and rice sheath blight, providing an important scientific basis for the research and development of new pesticides.

The utility model is suitable for the technical field of waxberry tree planting, and provides a waxberry tree bark split disease prevention and treatment device which comprises a containing box used for containing pesticide for preventing and treating the waxberry tree bark split disease. The collecting bottle is assembled on one side of the placing box; the mounting frame is arranged on the outer wall of one side of the collecting bottle; the scraper is fixedly mounted on the mounting frame and is used for scraping off barks at lesion positions of the waxberry trees; and the collecting mechanism is assembled on the collecting bottle and is used for collecting chippings generated when the scraping knife is used for scraping the bark of the waxberry tree. According to the prevention and treatment device for the bark cracking disease of the waxberry tree, the bark cracking disease of the waxberry tree can be prevented and treated, diseased bark chippings generated by scraping and cleaning diseased bark can be collected, the situation that the bark cracking disease diseased bark chippings fly and adhere to other positions of the waxberry tree is reduced, then the situation that the bark cracking disease is spread is reduced, and the service life of the waxberry tree is prolonged. Therefore, the effect of preventing and treating the red bayberry tree bark cracking disease is improved.

The invention discloses a primer probe combination for simultaneously detecting four tomato pathogens, a kit and application thereof, and belongs to the technical field of molecular biological detection. The invention aims to solve the technical problems that the existing molecular biological detection method for tomato diseases cannot simultaneously detect various tomato pathogens or cannot simultaneously detect bacterial pathogens and virus pathogens of tomatoes and is long in time consumption and the like. The invention provides a primer probe combination for detecting tomato canker pathogen, New Delhi tomato leaf curl virus, tomato spotted wilf virus and tomato brown wrinkled fruit virus in tomato or pepper crops, an integrated micro-fluidic chip kit containing the primer probe combination and a detection method. The method for detecting pathogens in tomato or pepper crops by adopting the primer probe combination and the kit provided by the invention has the advantages of higher sensitivity and accuracy, simplicity and convenience in operation, rapidness and time saving. The primer probe combination, the kit and the method provided by the invention can simultaneously detect the four pathogens, and play an important role in on-site rapid detection of the pathogens in tomato or pepper planting.

The invention discloses a tricyclic compound containing an indole structure as well as a composition and agricultural application of the tricyclic compound, and particularly relates to a tricyclic compound of optionally substituted or unsubstituted C4-7-membered saturated and unsaturated alkyl and alkyl impurity groups of indole and a composition and pesticide application of the tricyclic compound of optionally substituted or unsubstituted C4-7-membered saturated and unsaturated alkyl and alkyl impurity groups of indole. The compound provided by the invention has good inhibitory activity on plant pathogenic bacteria and fungi, particularly, the compound A-10 shows broad-spectrum and excellent inhibitory activity on test strains, and EC50 of the compound A-10 on Xanthomonas oryzae pv. Oryzae, Xanthomonas citri pv. Citri and Xanthomonas syringae pv. Kiwiis is 8.55 mu g / mL, 2.70 mu g / mL and 9.89 mu g / mL respectively.

The application discloses a method, reagent and application for identifying or tracing citrus canker bacteria. The method comprises the following steps: obtaining seven gene sequences of the to-be-tested citrus canker bacteria, performing multi-locus sequence typing analysis on the seven gene sequences, determining alleles and numbering, concatenating the allele numbers of the seven genes to serve as a sequence type, comparing the sequence type with a reference strain sequence type, and obtaining tracing information of the to-be-tested strain; the reference strain is a citrus canker bacteria with known tracing information; the seven genes are XAC_RS07980, XAC_RS21550, copB, egl, fliL, hrpA and pliY. The seven housekeeping genes suitable for multi-locus sequence typing of the citrus canker bacteria are used for tracing detection, different geographical source strains can be distinguished at high resolution, and the method has important significance for strain regional research, evolution, transmission, disease prevalence and prevention and treatment. The method can be used for identifying and tracing the citrus canker bacteria.

This invention relates to a class of compounds containing isopropanolamine substructures, their preparation methods, and applications. These compounds have the structure shown in general formula (I). Based on 4-chlorothiophenol, this invention introduces a nitrogen-containing fragment into this system to synthesize a series of compounds containing isopropanolamine substructures. These compounds exhibit excellent inhibitory effects on plant pathogenic bacteria such as *Bacillus thuringiensis*, *Citrus canker*, *Rhizoctonia solani*, and *Ophiopogon japonicus*.

The invention discloses an application of any compound of an Oxoapophine alkaloid derivative in prevention and treatment of agricultural diseases. A biological activity test shows that the derivative shows certain inhibitory activity on two plant pathogenic bacteria such as rice bacterial blight and citrus canker pathogen, and five plant pathogenic fungi such as rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea, fusarium graminearum, magnaporthe grisea and wheat stem rot; the compound has a good inhibition effect on citrus canker pathogen and sclerotinia sclerotiorum. The compound is low in preparation difficulty, raw materials are cheap and easy to obtain, and the compound is expected to be developed into a novel agricultural antibacterial agent.

The invention discloses application of tetrathiafulvalene as a bactericide in bacterial diseases and the bactericide, and relates to the technical field of food industry and agriculture. The invention relates to application of tetrathiafulvalene as a bactericide in bacterial diseases and the bactericide. The bacterial diseases comprise diseases caused by plant pathogenic bacteria and / or food-borne pathogenic bacteria. The invention aims to solve the problem of disease control in food industry and agriculture, and provides new application of tetrathiafulvalene in bacterial diseases. The invention provides application of tetrathiafulvalene in preventing and treating agricultural bacterial diseases caused by rice bacterial blight pathogenic bacteria, citrus ulcer pathogenic bacteria and pseudomonas solanacearum pathogenic bacteria and food pollution caused by escherichia coli, bacillus cereus, listeria monocytogenes, salmonella enteritidis and the like.

This invention relates to a topical agent for preventing and treating poplar canker and its application method. The topical agent, by weight percentage, comprises the following ingredients: 0.1-0.2% pyraclostrobin; 1-3% wetting and dispersing agent; 0.2%-0.8% thickener; 2-4% antifreeze agent; 0.01%-0.3% micronutrient fertilizer; 0.1%-0.3% defoamer; and the balance being deionized water. Before application, first use a scraper to clean the ulcerated areas on the poplar branches and trunks, thoroughly scraping away the rotten tissue, extending 2-3 cm beyond the diseased area on both the upper and lower surfaces. Then, evenly and promptly apply the topical agent to the treated wound surface, cover it with breathable non-woven fabric, and then apply another layer of topical agent over the non-woven fabric, ensuring close adhesion to the branches and trunk. The topical agent of this invention is simple to use, highly effective, and can effectively control canker, reduce the impact on poplar growth, and achieve efficient prevention and treatment of poplar canker.

The invention discloses a quinoline derivative containing an alkenyl structure as well as a preparation method and application of the quinoline derivative, the compound has a structure as shown in a general formula (I), and the compound has a specific effect on plant pathogenic bacteria such as Xanthomonas oryzae pv. Oryzae, Xanthomonas citri pv. Citri and Xanthomonas syringae pv. Kiwifruit; the plant pathogenic fungi such as rhizoctonia solani, botrytis cinerea, eggplant verticillium wilt and the like can be inhibited. All the compounds show excellent inhibitory activity on Xanthomonas oryzae pv. Oryzae and Xanthomonas citri pv. Citri, the EC50 of the compounds is 0.024-6.524 [mu] g / mL and 0.074-8.718 [mu] g / mL respectively, part of the compounds still have good inhibitory activity on Xanthomonas citri pv. Citri, the EC50 of the compounds is 2.036-10.776 [mu] g / mL, and the compounds can be used for preparing pesticides for resisting plant pathogenic bacteria.

The invention discloses an application of N-phenylmaleimide as a synergist of a copper-based bactericide, and finds for the first time that the N-phenylmaleimide and the copper-based bactericide are mixed for use, so that the dosage of the copper-based bactericide can be reduced, and meanwhile, good bactericidal activity can be ensured; and verification is carried out on mango bacterial angular leaf spot bacteria and citrus canker bacteria, and the N-phenylmaleimide (NPM) shows a remarkable synergistic effect when being mixed with the copper-based bactericide. The use amount of the copper-based bactericide in unit area is reduced, the accumulation risk of heavy metals in soil is reduced, resistance evolution of pathogenic bacteria to copper is delayed, and the method has important significance for solving the increasingly prominent drug resistance problem of pathogenic bacteria.

A new cherry tree particularly distinguished by red, flat, round fruit, with a highly precocious fruiting habit, self-fertility, less susceptible to rain crack and moderate resistance to bacterial canker, is disclosed.

The present invention belongs to the technical field of plant disease prevention and control, and specifically relates to a guanidine-containing phenylfuran carboxamide compound and its application. Experiments have shown that the guanidine-containing phenylfuran carboxamide compound has a significant inhibitory effect on Xanthomonas such as rice bacterial leaf blight, rice bacterial leaf spot, citrus canker, and crucifer black rot, both in vivo and in vitro. It is further found that the compound has a good binding affinity with histidinol dehydrogenase in bacteria, and can inhibit the degradation of histidine by binding to histidinol dehydrogenase, thereby inhibiting bacterial growth. The experimental results also show that histidinol dehydrogenase can be used as a target for inhibiting the activity of Xanthomonas, and is used to further screen high-activity inhibitors.

The invention relates to an isopropanolamine pyridine ether compound as well as a preparation method and application thereof. The compound has a structure as shown in a general formula (I): # imgabs0 #. According to the invention, 2, 3-dichloropyridine and hydroquinone are taken as leader, and a series of isopropanolamine pyridine ether compounds are synthesized through three steps; the compound has a good inhibition effect on plant pathogenic bacteria such as Xanthomonas oryzae pv. Oryzae, Xanthomonas oryzae pv. Oryzae, Xanthomonas citri pv. Citri, Xanthomonas syringae pv. Kiwifruit and the like.

This invention belongs to the field of microbial technology, specifically relating to a strain of Streptomyces steviolus. Streptomyces stephensoniae Its applications. The *Streptomyces steffensenii* NM-1 strain is deposited at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC M 20253020, and its 16S rDNA sequence is shown in SEQ ID NO:1. This strain produces hydrocyanic acid and secretes cellulase, chitinase, protease, and lipase, and can grow in a wide range of environments from pH 5.0 to 12.0 and temperature 10 to 35℃. Plate confrontation experiments showed that this strain has significant inhibitory effects on various plant pathogenic fungi, including *Fusarium wilt* of tomato, *Fusarium graminearum* of wheat, *Anthracnose* of pepper, *Fusarium canker* of walnut, *Fusarium wilt* of cucumber, and *Fusarium root rot* of garlic. Pot experiments confirmed that the fermentation broth of this strain achieved a control efficacy of 74.35% against *Fusarium wilt* of tomato. This invention enriches the strain resource bank and shows promising application prospects in the green control of fungal diseases in crops.

The invention discloses an anti-streptomycin gene as well as an encoding protein and application thereof, and belongs to the technical field of phytopathology. The invention discloses a novel streptomycin resistance gene aphh (3) derived from tomato canker pathogen. The gene is located on a newly found plasmid pCM3 of tomato canker pathogen, and is used for coding a protein APH (3) belonging to an aminoglycoside antibiotic phosphotransferase family. The functions of the gene are verified through tests of gene knockout, function complementation, heterologous expression and the like, meanwhile, mass spectrometric analysis proves that APH (3) can catalyze streptomycin molecules to generate phosphorylation reaction to generate streptomycin monophosphate, so that the inhibitory activity on bacteria is lost, and a strain carrying the gene has remarkable resistance to streptomycin. The streptomycin resistance gene aphh (3) can be widely applied to the fields of pathogenic bacterium resistance mechanism research, resistance strain screening, molecular marker selection and the like, and has important scientific research and application values.

The application discloses a benzopentaheterocyclic compound containing a hydrazine structure and an application thereof. The compound, stereoisomers of the compound, or a salt of the compound, or a solvate of the compound has good inhibiting effects on plant pathogenic bacteria and fungi. The compound has good inhibiting effects on fungi such as Colletotrichum gloeosporioides, Colletotrichum theae-sinensis, Mycosphaerella pachyrrhizi, Alternaria solani, Phytophthora capsici and the like, and pathogenic bacteria such as Xanthomonas oryzae, Xanthomonas citri and the like. Some of the compounds show excellent inhibiting activities on Colletotrichum theae-sinensis, with an EC 50 of 11.85 μg / mL. Some of the compounds show excellent inhibiting activities on Mycosphaerella pachyrrhizi, with an EC 50 of 2.88-16.41 μg / mL. Some of the compounds show excellent inhibiting activities on Xanthomonas citri, with a primary screening activity of 13.69-27.03 μg / mL, which is superior to a commercial drug, copper oxychloride. Some of the compounds show excellent inhibiting activities on Xanthomonas campestris, with a primary screening activity of 25.07-43.78 μg / mL, which is superior to the commercial drug, copper oxychloride. The application provides an important scientific basis for the research and development of new pesticides.

The invention relates to a preparation method of a supernatant for biological control, which comprises the following steps: fermenting and centrifuging streptomyces fusiformis, and taking the supernatant; the strain name of the streptomyces fusiformis is streptomyces fusiformis JN4-2, and the preservation number of the streptomyces fusiformis JN4-2 is CGMCC (China General Microbiological Culture Collection Center) No. 28157. The supernate can be used for treating Korean pine rhizoctonia solani, red adzuki bean colletotrichum gloeosporioides, symbiotic microporus, apple shell monochromatic alternaria solani, exserohilum turcicum, tobacco target leaf spot, fusarium moniliforme, muskmelon vine cutting pathogen, pepper colletotrichum gloeosporioides, dahurian larch shoot pathogen, blueberry canker pathogen, poplar skin rot pathogen and valsa mali. The biological control agent can be used for biologically controlling pathogenic bacteria such as poplar canker pathogen, eggplant brown rot pathogen, pythium aphanidermatum, alternaria alternata, blueberry branch blight pathogen, apple fruit rot pathogen, cucumber sclerotinia sclerotiorum and the like, and has the advantages of good biocontrol effect, wide antibacterial spectrum, wide application range and the like.

This invention discloses a DNA barcode sequence and its applications, belonging to the field of microbial identification technology. This invention provides a method for identifying *Tomato Canker* pathogens. Clavibacter michiganensis A novel DNA barcode sequence with high specificity for identification. Phylogenetic analysis showed that in the phylogenetic tree constructed based on the DNA barcode sequence described in this invention, all tested species formed independent monophyletic groups with high support. Further sequence alignment analysis showed that the amplified sequences of each species all possessed their unique SNP sites. These results confirm that the DNA barcode provided by this invention can accurately distinguish *Tomato Canker* pathogens. Clavibacter michiganensis It can also effectively identify Clavibacter The other species within the genus demonstrate its strong species-level resolution.