36 results about "Canker" patented technology

This invention discloses a class of thiadiazole-benzohexacyclic compounds, their preparation methods, and applications. The structural formula is shown in Formula I. This invention introduces disulfide bond fragments into the thiadiazole-benzohexacyclic skeleton to obtain a novel class of thiadiazole-benzohexacyclic compounds. These compounds have a novel structure, a simple preparation method, readily available raw materials, and are unlikely to produce harmful byproducts. The compounds exhibit inhibitory effects against various plant pathogenic bacteria, particularly against *Citrus canker*, *Ralstonia solanacearum*, and *Bacterial angular leaf spot* of cucumber, demonstrating significant inhibitory effects. They can be used as agricultural bactericides and have great application value.

The invention discloses an application of trichoderma harzianum in biological prevention and treatment of wild apple tree canker, the classification name of the trichoderma harzianum is Bacillus velezensis Rifai, the preservation unit is China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO. 42231, and the preservation time is September 29, 2025. Experimental results show that the trichoderma harzianum has remarkable antagonistic activity on parasitic cyst, apple cyst, nutshell cyst, cyst hormaechei, prunus cyst and pear cyst, is environment-friendly and pollution-free, and also provides a safe and reliable new way for preventing and treating the valsa mali of the wild apples; in addition, the research also finds that the fermentation product of the trichoderma harzianum has a certain biocontrol effect on the in-vitro branches infected by the physosporium piricola, so that the trichoderma harzianum and the fermentation product of the trichoderma harzianum provided by the invention can be used for preventing and treating the wild apple tree canker, and have good development and application prospects.

This invention relates to the field of medicinal chemistry, specifically to a capsaicin-like compound containing isopropanolamine, its preparation method, and its application. This type of compound has a structure as shown in general formula (I). The invention uses capsaicin and isopropanolamine as pharmacophores, connecting the two pharmacophores with different bridging chains to synthesize a series of capsaicin-like derivatives containing isopropanolamine. These compounds exhibit excellent inhibitory effects on plant pathogenic bacteria such as rice bacterial blight, citrus canker, rice bacterial leaf streak, and kiwifruit canker; and plant pathogenic fungi such as cucumber gray mold, pepper wilt, rapeseed sclerotinia rot, eggplant verticillium wilt, wheat scab, potato late blight, blueberry root rot, grape coccidioidomyces, dragon fruit anthracnose, and rice sheath blight, providing an important scientific basis for the research and development of new pesticides.

The utility model is suitable for the technical field of waxberry tree planting, and provides a waxberry tree bark split disease prevention and treatment device which comprises a containing box used for containing pesticide for preventing and treating the waxberry tree bark split disease. The collecting bottle is assembled on one side of the placing box; the mounting frame is arranged on the outer wall of one side of the collecting bottle; the scraper is fixedly mounted on the mounting frame and is used for scraping off barks at lesion positions of the waxberry trees; and the collecting mechanism is assembled on the collecting bottle and is used for collecting chippings generated when the scraping knife is used for scraping the bark of the waxberry tree. According to the prevention and treatment device for the bark cracking disease of the waxberry tree, the bark cracking disease of the waxberry tree can be prevented and treated, diseased bark chippings generated by scraping and cleaning diseased bark can be collected, the situation that the bark cracking disease diseased bark chippings fly and adhere to other positions of the waxberry tree is reduced, then the situation that the bark cracking disease is spread is reduced, and the service life of the waxberry tree is prolonged. Therefore, the effect of preventing and treating the red bayberry tree bark cracking disease is improved.

The invention discloses a primer probe combination for simultaneously detecting four tomato pathogens, a kit and application thereof, and belongs to the technical field of molecular biological detection. The invention aims to solve the technical problems that the existing molecular biological detection method for tomato diseases cannot simultaneously detect various tomato pathogens or cannot simultaneously detect bacterial pathogens and virus pathogens of tomatoes and is long in time consumption and the like. The invention provides a primer probe combination for detecting tomato canker pathogen, New Delhi tomato leaf curl virus, tomato spotted wilf virus and tomato brown wrinkled fruit virus in tomato or pepper crops, an integrated micro-fluidic chip kit containing the primer probe combination and a detection method. The method for detecting pathogens in tomato or pepper crops by adopting the primer probe combination and the kit provided by the invention has the advantages of higher sensitivity and accuracy, simplicity and convenience in operation, rapidness and time saving. The primer probe combination, the kit and the method provided by the invention can simultaneously detect the four pathogens, and play an important role in on-site rapid detection of the pathogens in tomato or pepper planting.

The invention discloses a tricyclic compound containing an indole structure as well as a composition and agricultural application of the tricyclic compound, and particularly relates to a tricyclic compound of optionally substituted or unsubstituted C4-7-membered saturated and unsaturated alkyl and alkyl impurity groups of indole and a composition and pesticide application of the tricyclic compound of optionally substituted or unsubstituted C4-7-membered saturated and unsaturated alkyl and alkyl impurity groups of indole. The compound provided by the invention has good inhibitory activity on plant pathogenic bacteria and fungi, particularly, the compound A-10 shows broad-spectrum and excellent inhibitory activity on test strains, and EC50 of the compound A-10 on Xanthomonas oryzae pv. Oryzae, Xanthomonas citri pv. Citri and Xanthomonas syringae pv. Kiwiis is 8.55 mu g / mL, 2.70 mu g / mL and 9.89 mu g / mL respectively.

The application discloses a method, reagent and application for identifying or tracing citrus canker bacteria. The method comprises the following steps: obtaining seven gene sequences of the to-be-tested citrus canker bacteria, performing multi-locus sequence typing analysis on the seven gene sequences, determining alleles and numbering, concatenating the allele numbers of the seven genes to serve as a sequence type, comparing the sequence type with a reference strain sequence type, and obtaining tracing information of the to-be-tested strain; the reference strain is a citrus canker bacteria with known tracing information; the seven genes are XAC_RS07980, XAC_RS21550, copB, egl, fliL, hrpA and pliY. The seven housekeeping genes suitable for multi-locus sequence typing of the citrus canker bacteria are used for tracing detection, different geographical source strains can be distinguished at high resolution, and the method has important significance for strain regional research, evolution, transmission, disease prevalence and prevention and treatment. The method can be used for identifying and tracing the citrus canker bacteria.

This invention relates to a class of compounds containing isopropanolamine substructures, their preparation methods, and applications. These compounds have the structure shown in general formula (I). Based on 4-chlorothiophenol, this invention introduces a nitrogen-containing fragment into this system to synthesize a series of compounds containing isopropanolamine substructures. These compounds exhibit excellent inhibitory effects on plant pathogenic bacteria such as *Bacillus thuringiensis*, *Citrus canker*, *Rhizoctonia solani*, and *Ophiopogon japonicus*.

The invention discloses an application of any compound of an Oxoapophine alkaloid derivative in prevention and treatment of agricultural diseases. A biological activity test shows that the derivative shows certain inhibitory activity on two plant pathogenic bacteria such as rice bacterial blight and citrus canker pathogen, and five plant pathogenic fungi such as rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea, fusarium graminearum, magnaporthe grisea and wheat stem rot; the compound has a good inhibition effect on citrus canker pathogen and sclerotinia sclerotiorum. The compound is low in preparation difficulty, raw materials are cheap and easy to obtain, and the compound is expected to be developed into a novel agricultural antibacterial agent.

The invention discloses application of tetrathiafulvalene as a bactericide in bacterial diseases and the bactericide, and relates to the technical field of food industry and agriculture. The invention relates to application of tetrathiafulvalene as a bactericide in bacterial diseases and the bactericide. The bacterial diseases comprise diseases caused by plant pathogenic bacteria and / or food-borne pathogenic bacteria. The invention aims to solve the problem of disease control in food industry and agriculture, and provides new application of tetrathiafulvalene in bacterial diseases. The invention provides application of tetrathiafulvalene in preventing and treating agricultural bacterial diseases caused by rice bacterial blight pathogenic bacteria, citrus ulcer pathogenic bacteria and pseudomonas solanacearum pathogenic bacteria and food pollution caused by escherichia coli, bacillus cereus, listeria monocytogenes, salmonella enteritidis and the like.

This invention relates to a topical agent for preventing and treating poplar canker and its application method. The topical agent, by weight percentage, comprises the following ingredients: 0.1-0.2% pyraclostrobin; 1-3% wetting and dispersing agent; 0.2%-0.8% thickener; 2-4% antifreeze agent; 0.01%-0.3% micronutrient fertilizer; 0.1%-0.3% defoamer; and the balance being deionized water. Before application, first use a scraper to clean the ulcerated areas on the poplar branches and trunks, thoroughly scraping away the rotten tissue, extending 2-3 cm beyond the diseased area on both the upper and lower surfaces. Then, evenly and promptly apply the topical agent to the treated wound surface, cover it with breathable non-woven fabric, and then apply another layer of topical agent over the non-woven fabric, ensuring close adhesion to the branches and trunk. The topical agent of this invention is simple to use, highly effective, and can effectively control canker, reduce the impact on poplar growth, and achieve efficient prevention and treatment of poplar canker.

The invention discloses a quinoline derivative containing an alkenyl structure as well as a preparation method and application of the quinoline derivative, the compound has a structure as shown in a general formula (I), and the compound has a specific effect on plant pathogenic bacteria such as Xanthomonas oryzae pv. Oryzae, Xanthomonas citri pv. Citri and Xanthomonas syringae pv. Kiwifruit; the plant pathogenic fungi such as rhizoctonia solani, botrytis cinerea, eggplant verticillium wilt and the like can be inhibited. All the compounds show excellent inhibitory activity on Xanthomonas oryzae pv. Oryzae and Xanthomonas citri pv. Citri, the EC50 of the compounds is 0.024-6.524 [mu] g / mL and 0.074-8.718 [mu] g / mL respectively, part of the compounds still have good inhibitory activity on Xanthomonas citri pv. Citri, the EC50 of the compounds is 2.036-10.776 [mu] g / mL, and the compounds can be used for preparing pesticides for resisting plant pathogenic bacteria.

The invention discloses an application of N-phenylmaleimide as a synergist of a copper-based bactericide, and finds for the first time that the N-phenylmaleimide and the copper-based bactericide are mixed for use, so that the dosage of the copper-based bactericide can be reduced, and meanwhile, good bactericidal activity can be ensured; and verification is carried out on mango bacterial angular leaf spot bacteria and citrus canker bacteria, and the N-phenylmaleimide (NPM) shows a remarkable synergistic effect when being mixed with the copper-based bactericide. The use amount of the copper-based bactericide in unit area is reduced, the accumulation risk of heavy metals in soil is reduced, resistance evolution of pathogenic bacteria to copper is delayed, and the method has important significance for solving the increasingly prominent drug resistance problem of pathogenic bacteria.

A new cherry tree particularly distinguished by red, flat, round fruit, with a highly precocious fruiting habit, self-fertility, less susceptible to rain crack and moderate resistance to bacterial canker, is disclosed.

This invention belongs to the field of microbial technology, specifically relating to a strain of Streptomyces steviolus. Streptomyces stephensoniae Its applications. The *Streptomyces steffensenii* NM-1 strain is deposited at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC M 20253020, and its 16S rDNA sequence is shown in SEQ ID NO:1. This strain produces hydrocyanic acid and secretes cellulase, chitinase, protease, and lipase, and can grow in a wide range of environments from pH 5.0 to 12.0 and temperature 10 to 35℃. Plate confrontation experiments showed that this strain has significant inhibitory effects on various plant pathogenic fungi, including *Fusarium wilt* of tomato, *Fusarium graminearum* of wheat, *Anthracnose* of pepper, *Fusarium canker* of walnut, *Fusarium wilt* of cucumber, and *Fusarium root rot* of garlic. Pot experiments confirmed that the fermentation broth of this strain achieved a control efficacy of 74.35% against *Fusarium wilt* of tomato. This invention enriches the strain resource bank and shows promising application prospects in the green control of fungal diseases in crops.

The invention discloses an anti-streptomycin gene as well as an encoding protein and application thereof, and belongs to the technical field of phytopathology. The invention discloses a novel streptomycin resistance gene aphh (3) derived from tomato canker pathogen. The gene is located on a newly found plasmid pCM3 of tomato canker pathogen, and is used for coding a protein APH (3) belonging to an aminoglycoside antibiotic phosphotransferase family. The functions of the gene are verified through tests of gene knockout, function complementation, heterologous expression and the like, meanwhile, mass spectrometric analysis proves that APH (3) can catalyze streptomycin molecules to generate phosphorylation reaction to generate streptomycin monophosphate, so that the inhibitory activity on bacteria is lost, and a strain carrying the gene has remarkable resistance to streptomycin. The streptomycin resistance gene aphh (3) can be widely applied to the fields of pathogenic bacterium resistance mechanism research, resistance strain screening, molecular marker selection and the like, and has important scientific research and application values.

The application discloses a benzopentaheterocyclic compound containing a hydrazine structure and an application thereof. The compound, stereoisomers of the compound, or a salt of the compound, or a solvate of the compound has good inhibiting effects on plant pathogenic bacteria and fungi. The compound has good inhibiting effects on fungi such as Colletotrichum gloeosporioides, Colletotrichum theae-sinensis, Mycosphaerella pachyrrhizi, Alternaria solani, Phytophthora capsici and the like, and pathogenic bacteria such as Xanthomonas oryzae, Xanthomonas citri and the like. Some of the compounds show excellent inhibiting activities on Colletotrichum theae-sinensis, with an EC 50 of 11.85 μg / mL. Some of the compounds show excellent inhibiting activities on Mycosphaerella pachyrrhizi, with an EC 50 of 2.88-16.41 μg / mL. Some of the compounds show excellent inhibiting activities on Xanthomonas citri, with a primary screening activity of 13.69-27.03 μg / mL, which is superior to a commercial drug, copper oxychloride. Some of the compounds show excellent inhibiting activities on Xanthomonas campestris, with a primary screening activity of 25.07-43.78 μg / mL, which is superior to the commercial drug, copper oxychloride. The application provides an important scientific basis for the research and development of new pesticides.

This invention discloses a DNA barcode sequence and its applications, belonging to the field of microbial identification technology. This invention provides a method for identifying *Tomato Canker* pathogens. Clavibacter michiganensis A novel DNA barcode sequence with high specificity for identification. Phylogenetic analysis showed that in the phylogenetic tree constructed based on the DNA barcode sequence described in this invention, all tested species formed independent monophyletic groups with high support. Further sequence alignment analysis showed that the amplified sequences of each species all possessed their unique SNP sites. These results confirm that the DNA barcode provided by this invention can accurately distinguish *Tomato Canker* pathogens. Clavibacter michiganensis It can also effectively identify Clavibacter The other species within the genus demonstrate its strong species-level resolution.

This document describes a bacterial composition and its use for controlling, suppressing, and / or preventing infection of tomatoes with the tomato canker fungus (Cmm). The bacterial composition comprises an aqueous mixture of live Bacillus subtilis and live Bacillus pumilus. The bacterial composition can be combined with existing herbicides, insecticides, mycicides, bactericidal agents, and nutrients, including but not limited to copper-containing compounds. Combination with copper mycicides such as Kocide® imparts synergistic efficacy.

The invention discloses a chemical preparation method and application of a 2-substituted-1-H-indole oxadiazole amine derivative, a series of compounds which take a urea or thiourea fragment as a linker and are substituted by different phenyl groups are synthesized on the basis of the structure of the 2-substituted-1-H-indole oxadiazole amine derivative, and the compounds have a good inhibition effect on pathogenic bacteria and can be used for preparing a novel compound for treating pathogenic bacteria. The compound has a good inhibition effect on pathogenic bacteria such as xanthomonas oryzae pv. Oryzae, citrus canker pathogen, kiwifruit bacterial canker pathogen and ralstonia solanacearum, and provides an important scientific basis for research, development and creation of new pesticides. The compound has a structure shown in the following general formula.

The invention belongs to the technical field of green prevention and control of plant diseases, and particularly relates to a method for preventing and controlling apple tree canker by using liquorice. The invention provides a method for preventing and treating apple tree canker by using liquorice. The method comprises the following steps: (1) planting liquorice as raw grass between rows of an apple orchard; (2) after harvesting liquorice, juicing to obtain liquorice juice; (3) spraying the liquorice juice to the apple trees, and spraying once every 10-20 days; and (4) when the apple orchard is cleaned, turning and burying the residual liquorice to return to the field. The control effect of the liquorice juice on the apple tree canker is defined, and a material basis and an important scientific basis are provided for follow-up field control of the apple tree canker.

The invention discloses a culture medium for kiwifruit pollen canker pathogen and a detection method of the culture medium, and belongs to the technical field of phytopathogen separation and identification. The culture medium comprises a culture solution, ferric chloride and agar powder, the detection method for the kiwifruit pollen canker germs comprises the following steps: inoculating a specimen into the culture medium in claim 1, culturing, carrying out fluorescence detection on the culture medium, adding a chelating agent, continuously culturing, and carrying out fluorescence detection again. The preparation method of the chelating agent comprises the following steps: carrying out nucleophilic substitution reaction on branched polyethyleneimine and chloromethylated polystyrene resin to obtain an intermediate; and grafting pyridine-2, 6-dicarboxylic acid to the intermediate to obtain the chelating agent, and determining whether the canker pathogen belongs to the pseudomonas syringae or not according to the change of the suddenly increased fluorescence intensity.

This application relates to a strain of *Pseudomonas lundense* and its applications. The present invention isolates a strain of *Pseudomonas lundense* from the root soil of rice plants in a rice cultivation base. This strain is effective against rice basal rot pathogens (…). Dickeya oryzae ), potato soft rot fungus ( Dickeya dadantii ), and Taro soft rot fungus ( Dickeya fangzhongdai It has a good control effect on sugarcane smut ( ) Sporisorium scitamineum ), rice blast fungus ( Magnaporthe oryzae ), Neodatura spp. Neoscytalidium dimidiatum ), neatly arranged small sclerotia ( Sclerotium rolfsii ), Lithocarpus ( Calonectria ), Black spores ( Nigrospora lacticoloni a) Ralstonia solanacearum ( Ralstonia solanacearum Xanthomonas aeruginosa (), Xanthomonas campestris pv. Campestris ) and citrus canker pathogen ( Xanthomonas citri subsp. citri It also has a good control effect. Therefore, it can be widely used in the control of plant diseases, including rice basal rot.

The invention discloses bacillus siamensis and application thereof, and belongs to the technical field of microorganisms. The bacillus siamensis EfA-3 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on November 13, 2024, and the preservation number is CGMCC NO.31710. The bacillus siamensis EfA-3 is a bacillus siamensis EfA-3. Experiments prove that the strain EfA-3 simultaneously has the functions of inhibiting five plant pathogenic fungi (phytophthora nicotianae, fusarium oxysporum, alternaria alternata, sclerotium rolfsii and fusarium graminearum) and three plant pathogenic bacteria (ralstonia solanacearum, carrot soft rot solidobacterium and citrus canker pathogen) and poison-killing prodenia litura, so that the strain EfA-3 has the effects of inhibiting the five plant pathogenic fungi (phytophthora nicotianae, fusarium oxysporum, alternaria alternata, sclerotium rolfsii and citrus canker pathogen). The technical blank of multifunctional biocontrol of bacillus siamensis is filled, and a new resource is provided for development of a low-cost biological control preparation.

The invention discloses application of a quinine derivative in prevention and treatment of agricultural pathogenic bacteria, and relates to the technical field of medicinal chemistry. A biological activity test shows that the quinine derivative has a significant effect on plant pathogenic bacteria such as Xanthomonas oryzae pv. Oryzae, Xanthomonas citri pv. Citri and tomato bacterial wilt, and plant pathogenic fungi such as rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea, magnaporthe oryzae, fusarium pseudograminearum and fusarium graminearum. And the compound shows certain inhibitory activity on decay-causing pathogenic fungi such as aspergillus flavus, penicillium expansum and brown rot of stone fruits, and has a relatively good inhibitory effect on pathogenic bacteria of rice bacterial blight and rice blast bacteria. The compound is low in preparation difficulty, raw materials are cheap and easy to obtain, and the compound is expected to be developed into a novel agricultural antibacterial agent.

The application discloses a quinina derivative for preventing and treating agricultural diseases, a preparation method and application thereof, and relates to a compound with a structural formula as shown in formula I, or a pharmaceutically acceptable salt, a solvate and a tautomer thereof. It is found that the quinina derivative has excellent inhibitory effect on Xanthomonas oryzae and Xanthomonas citri, has great development value in treating plant pathogenic bacteria, and provides more choices for preventing and treating agricultural diseases.

The present application relates to a kind of toxin type plant rooting liquid, rooting method and application, wherein the composition of rooting liquid is poplar canker bacteria toxin, formula is 5.00% poplar canker bacteria toxin aqueous solution, using method is the base of the stem section to be rooted into the rooting liquid, when the stem section base appears root primordium, transplant to soil, rooting rate can reach 83.33%, and more fibrous root, plant growth is robust, seedling survival rate can reach 83.33%, simultaneously, the rooting liquid has the ability of enhancing seedling disease resistance simultaneously.This application not only can efficiently promote plant rooting, improve transplanting survival rate, but also can improve the immunity of plant, low cost, simple to make, convenient to use, remarkable effect.

This invention belongs to the field of biopesticide technology and discloses a plant immune inducer containing cyclic diguanylic acid (cDGA) and its application. The final concentration of cDGA in this plant immune inducer is 0.5~1 μmol / L. After spraying, it can rapidly activate multiple immune responses in plants, including reactive oxygen species (ROS) bursts, callose accumulation, and efficient expression of disease resistance genes and ROS scavenging-related genes. By activating the plant's own immune system, it enhances the plant's resistance to various pathogens, showing significant control effects against bacterial spot fungus of tomato, Phytophthora, and Actinidia kiwifruit canker. This cDGA plant immune inducer also has advantages such as being environmentally friendly, low in toxicity, residue-free, and unlikely to induce drug resistance in pathogens, thus having broad application prospects.

The application provides a cinnamoyl-substituted unsaturated nitrogen-containing heterocyclic oxygen-containing compound, a composition, a preparation method and application, and belongs to the technical field of medicinal chemistry.The structural formula of the compound provided by the application is The application takes a cinnamic acid structure as a lead compound, then takes a nitrogen-containing heterocyclic ring such as piperazine, piperidine and homopiperazine as a connecting group, and on this basis, different pharmacophores are connected to synthesize a series of cinnamoyl-substituted unsaturated nitrogen-containing heterocyclic oxygen-containing compounds.The compounds have excellent inhibitory effect on plant pathogenic bacteria such as rice bacterial leaf blight bacteria, citrus canker bacteria and kiwi canker bacteria.