8 results about "Canker" patented technology

This invention discloses a class of thiadiazole-benzohexacyclic compounds, their preparation methods, and applications. The structural formula is shown in Formula I. This invention introduces disulfide bond fragments into the thiadiazole-benzohexacyclic skeleton to obtain a novel class of thiadiazole-benzohexacyclic compounds. These compounds have a novel structure, a simple preparation method, readily available raw materials, and are unlikely to produce harmful byproducts. The compounds exhibit inhibitory effects against various plant pathogenic bacteria, particularly against *Citrus canker*, *Ralstonia solanacearum*, and *Bacterial angular leaf spot* of cucumber, demonstrating significant inhibitory effects. They can be used as agricultural bactericides and have great application value.

This invention relates to a topical agent for preventing and treating poplar canker and its application method. The topical agent, by weight percentage, comprises the following ingredients: 0.1-0.2% pyraclostrobin; 1-3% wetting and dispersing agent; 0.2%-0.8% thickener; 2-4% antifreeze agent; 0.01%-0.3% micronutrient fertilizer; 0.1%-0.3% defoamer; and the balance being deionized water. Before application, first use a scraper to clean the ulcerated areas on the poplar branches and trunks, thoroughly scraping away the rotten tissue, extending 2-3 cm beyond the diseased area on both the upper and lower surfaces. Then, evenly and promptly apply the topical agent to the treated wound surface, cover it with breathable non-woven fabric, and then apply another layer of topical agent over the non-woven fabric, ensuring close adhesion to the branches and trunk. The topical agent of this invention is simple to use, highly effective, and can effectively control canker, reduce the impact on poplar growth, and achieve efficient prevention and treatment of poplar canker.

This invention belongs to the field of microbial technology, specifically relating to a strain of Streptomyces steviolus. Streptomyces stephensoniae Its applications. The *Streptomyces steffensenii* NM-1 strain is deposited at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC M 20253020, and its 16S rDNA sequence is shown in SEQ ID NO:1. This strain produces hydrocyanic acid and secretes cellulase, chitinase, protease, and lipase, and can grow in a wide range of environments from pH 5.0 to 12.0 and temperature 10 to 35℃. Plate confrontation experiments showed that this strain has significant inhibitory effects on various plant pathogenic fungi, including *Fusarium wilt* of tomato, *Fusarium graminearum* of wheat, *Anthracnose* of pepper, *Fusarium canker* of walnut, *Fusarium wilt* of cucumber, and *Fusarium root rot* of garlic. Pot experiments confirmed that the fermentation broth of this strain achieved a control efficacy of 74.35% against *Fusarium wilt* of tomato. This invention enriches the strain resource bank and shows promising application prospects in the green control of fungal diseases in crops.

This invention discloses a DNA barcode sequence and its applications, belonging to the field of microbial identification technology. This invention provides a method for identifying *Tomato Canker* pathogens. Clavibacter michiganensis A novel DNA barcode sequence with high specificity for identification. Phylogenetic analysis showed that in the phylogenetic tree constructed based on the DNA barcode sequence described in this invention, all tested species formed independent monophyletic groups with high support. Further sequence alignment analysis showed that the amplified sequences of each species all possessed their unique SNP sites. These results confirm that the DNA barcode provided by this invention can accurately distinguish *Tomato Canker* pathogens. Clavibacter michiganensis It can also effectively identify Clavibacter The other species within the genus demonstrate its strong species-level resolution.

This invention belongs to the field of biopesticide technology and discloses a plant immune inducer containing cyclic diguanylic acid (cDGA) and its application. The final concentration of cDGA in this plant immune inducer is 0.5~1 μmol / L. After spraying, it can rapidly activate multiple immune responses in plants, including reactive oxygen species (ROS) bursts, callose accumulation, and efficient expression of disease resistance genes and ROS scavenging-related genes. By activating the plant's own immune system, it enhances the plant's resistance to various pathogens, showing significant control effects against bacterial spot fungus of tomato, Phytophthora, and Actinidia kiwifruit canker. This cDGA plant immune inducer also has advantages such as being environmentally friendly, low in toxicity, residue-free, and unlikely to induce drug resistance in pathogens, thus having broad application prospects.

The present application relates to a Paenibacillus polymyxa strain Y31, a microbial inoculum prepared therefrom and applications thereof. The strain Y31 can effectively inhibit the growth of Dothiorella gregaria, and field control tests show that the comprehensive prevention and control effect of the microbial inoculum prepared from the strain Y31 on the disease is as high as 72% or more in the early stage of the disease. Antagonistic experiments show that the strain Y31 also has good bacteriostatic effect on Dothiorella septospora and Dothiorella needle red rot fungus, and is suitable for being used as a broad-spectrum antibacterial agent in the biocontrol practice of pine fungal diseases. Meanwhile, the strain Y31 also has obvious inhibitory effect on bacterial diseases such as willow bacterial wilt fungus, pear fire blight fungus, walnut bacterial black spot fungus and kiwi fruit canker fungus, and can be used for the prevention and control of diseases such as willow wilt. In addition, the strain Y31 also has functions such as nitrogen fixation, phosphorus and potassium solubilization, and has certain salt and alkali tolerance, and can be used for promoting growth and disease resistance of plants in saline-alkali land.

This invention discloses a class of thiothiadiazole-benzohexacyclic compounds, their preparation methods, and applications. The structural formula is shown in Formula I. This invention introduces thioether or disulfide bond fragments into the thiadiazole-benzohexacyclic skeleton to obtain a class of thiothiadiazole-benzohexacyclic compounds with novel structures. The preparation method is simple, uses readily available raw materials, and is unlikely to produce harmful byproducts. The compounds of this invention exhibit inhibitory effects on various plant pathogenic bacteria, especially against *Citrus canker*, *Ralstonia solanacearum*, and *Bacterial angular leaf spot* of cucumber, showing significant inhibitory effects. They can be used as agricultural bactericides and have great application value.