37 results about "Mottle" patented technology

The invention relates to the technical field of peach tree disease and insect pest control, in particular to a peach tree disease and insect pest control method and system.The method comprises the following steps that through peach orchard field investigation and survey, the number of branches is recorded, branch distribution and diseased leaf positions are detected, spot shapes and insect population cardinal numbers are shot, worm egg distribution and tree age information are summarized, and annual rings are checked; and measuring daily variation, soil nutrients and air temperature, and storing into a field table. According to the method, current state parameters of the peach trees are formed by recording key parameters such as the number and distribution of branches of the peach trees, diseased leaf positions, spot shapes, cardinal number of insects, tree age, moisture content, soil nutrients and air temperature, and a basis for dynamic monitoring of diseases and insect pests is established. On the basis of current situation parameter analysis, branch surface color spots, diseased leaf corrosion degree and fruit damage are compared, insect pest types are divided, parasitic characteristics are counted, abnormal data are further decomposed, and deep optimization of disease and insect pest classification and feature recognition is achieved.

The invention provides a molecular marker for detecting pepper light mottle virus L-series resistance genes and application of the molecular marker, and belongs to the technical field of genetic engineering, the molecular marker comprises SNP1 and SNP2, the SNP1 is a base T at the 2574th site of a yellow lantern pepper genome sequence with the NCBI login number of AB523370, and the SNP2 is a base T at the 2832nd site of the yellow lantern pepper genome sequence with the NCBI login number of AB523370; the specific gene sequence is a specific gene sequence of an L-series resistance gene L1c type gene, and the specific gene sequence is a basic group CA at the 2872 site to the 2873 site, a basic group TT at the 2877 site to the 2878 site, a basic group TG at the 2882 site to the 2883 site and a basic group AGA at the 2885 site to the 2887 site of the yellow lantern pepper with the NCBI accession number being AB523374. By utilizing the molecular marker and the specific sequence, the L-series resistance gene types of the yellow lantern pepper and the annual pepper can be rapidly and accurately detected, and the molecular marker can be used for molecular marker-assisted selective breeding.

The invention provides a method for removing capsicum light mottle viruses in capsicum seeds. The method comprises the following steps: (1) carrying out protease hydrolysis treatment on the capsicum seeds by adopting a protease aqueous solution capable of hydrolyzing virus protein shells; and (2) the pepper seeds treated in the step (1) are subjected to light irradiation in a solution containing a photosensitizer to excite the photosensitizer, so that the pepper seeds without the pepper mild mottle viruses are obtained, and the photosensitizer is a visible light excited photosensitizer. Compared with an existing PMMoV virus resisting method, the virus removing method has the advantages that PMMoV viruses of the pepper seeds can be effectively removed, the germination potential and the germination rate of the pepper seeds are not reduced, and the method is more efficient, economical, environmentally friendly and convenient to popularize; the method can promote the prevention and control of pepper virus diseases and the breeding of virus-free pepper seedlings, and guarantee the long-distance transportation of seeds.

The invention relates to a primer group and a kit for detecting watermelon silver mottle virus and application of the primer group and the kit. The primer group is a specific primer aiming at the NSs protein gene of the watermelon silver mottle virus. The detection method is a fluorescent quantitative PCR (Polymerase Chain Reaction) detection method based on an SYBR Green dye method. By applying the primer group, the kit and the detection method, the genome of the watermelon silver mottle virus can be efficiently and accurately quantitatively detected. Whether a sample contains the watermelon silver mottle virus or not can be rapidly and accurately judged through detection, and the specific content level of the watermelon silver mottle virus is determined; when the kit is applied to detection, the infection state and information of the watermelon silver mottle virus on the plant can be more accurately obtained, so that prevention and control can be carried out in time. The method has the advantages of simplicity in result judgment, convenience and rapidness in operation, high specificity and sensitivity, wide application prospect and the like.

A new and distinct Alocasia plant named ‘ELSTLALU’ particularly distinguished by a dark green background leaf coloration with irregular white to light green mottling and marbling, distinct mottled and marbled variegation distributed across most of the leaf surface, and a tall, upright growth habit with elongated petioles and an open appearance.

A new and distinct Rhaphidophora plant named ‘TUXRAP1’ particularly distinguished by its unique and uniformly mottled leaf variegation pattern colored yellowish grey.

The invention provides a primer group for detecting a tomato yellow mottle related virus TYMaV based on RT-RAA-CRISPR / Cas12a. The primer group comprises an RT-RAA amplification primer pair and a CRISPR / Cas12a detection probe. The invention provides a method for visually and rapidly detecting TYMaV. The method comprises the following steps: extracting total RNA (Ribonucleic Acid) of tobacco leaves infected by TYMaV or coarsely extracted virions; the method comprises the following steps: preparing RT-RAA reaction mixed liquid at the bottom of a PCR tube and CRISPR premixed liquid at the cover of the PCR tube, adding total RNA or crude extraction virus particles into the RT-RAA reaction mixed liquid, incubating in a 42 DEG C metal bath for 30 minutes, placing the CRISPR premixed liquid at the bottom of the tube through centrifugation or shaking, mixing with an RT-RAA reaction product, incubating in a 37 DEG C metal bath for 20 minutes, adding 80 mu L of ddH2O, and detecting a target strip by using a flow measurement chromatography test strip. The method can be used for preparing a kit for detecting TYMaV. According to the primer group and the established TYMaV visual rapid detection method based on RT-RAA-CRISPR / Cas12a, a theoretical basis and a technical guarantee are provided for monitoring, predicting, forecasting, preventing and treating the TYMaV in the field.

The invention belongs to the technical field of crop disease prevention and control, and particularly relates to a planting method for preventing cucumber fruit blotch germs and green mottle viruses, which comprises the following steps: acquiring soil and crop historical pathogen data, environment monitoring data and cucumber seed batch information of a planting area; querying supply chain quarantine records of the seeds, and carrying out standardization treatment on the seeds; after planting, the disease probability of each growth stage is predicted through a risk assessment algorithm, a dynamic monitoring plan is generated in combination with a fuzzy mapping algorithm, plant samples are collected, PCR detection of melon bacterial fruit blotch and RT-PCR detection of cucumber green mottle mosaic virus are carried out, and a quarantine report is issued. Based on the monitoring plan, the quarantine report and the real-time environment data, judging a comprehensive risk level, triggering a corresponding field intervention scheme, and recording an execution result to form an intervention log; after planting is finished, a prevention and control efficiency report is generated. Therefore, the problems of insufficient disease detection, lack of intervention measures and the like in the prior art are solved.

The invention belongs to the field of gene engineering, and discloses a zinnia elegans light mottle virus and application thereof. The invention provides a whole genome sequence of a potato Y virus (ZiMMV) newly identified on zinnia elegans, namely a ziMMV (ziMMV), a construction method of a virus expression vector based on the ZiMMV and an application of the virus expression vector based on the ZiMMV. The ZiMMV expression system constructed by the invention can realize efficient protein expression on compositae plants such as zinnia elegans and callistephus chinensis.

The present invention relates to a benzoxazole derivative containing a dithioacetal unit, its preparation method, and use. The compound of the present invention exhibits excellent antiviral activity against cucumber green mottle mosaic virus and other viruses, and can be used as a pharmaceutical or medicament for preventing and treating plant viral diseases such as cucumber green mottle mosaic. Its structure and preparation process are simple, and its production cost is low. The benzoxazole derivative containing a dithioacetal unit includes a benzoxazole compound containing a dithioacetal unit, or a stereoisomer thereof, or a salt or solvate thereof. The structural formula of the benzoxazole compound containing a dithioacetal unit is as follows: #imgabs0#

The invention relates to a method for monitoring the richness and morbidity of litchi pocking spot disease infection sources, and provides a simple and accurate litchi pocking spot disease prediction method for monitoring and early warning of litchi pocking spot disease latent infection sources in litchi producing areas of different areas. According to the method, the separation frequency of pathogenic bacteria of the asymptomatic fruit pocking spot disease and the severity of the field pocking spot disease are collected and detected regularly (one week) in the fruiting period, and the method can accurately monitor the richness of latent infection sources of the pocking spot disease. On the basis, the occurrence condition of the litchi pocking spot disease in the region in the current year can be predicted by combining weather forecast (rainfall days) of the local litchi fruit bearing period.

‘HUTAPD75’ is a distinctive Philodendron plant which is characterized by uniquely variegated laminas with a green base coloration, light yellow to golden yellow flecking, and irregular blotches of varying size and color, from near-white to yellow, as well as green petioles and petiole wings with a combination of near-white and light yellow irregular longitudinal stripes, and the stability of all characteristics from generation to generation.

The invention belongs to the technical field of plant virus molecular detection, particularly discloses a multiplex RT-PCR method and a kit for detecting important blueberry viruses, and particularly provides a multiplex RT-PCR detection primer for the blueberry viruses, and the sequence of the primer is shown as SEQ ID NO.1-12. The technical scheme provided by the invention is wide in detection range, high in specificity, high in specificity and high in sensitivity. The kit can be used for simultaneously detecting blueberry scorching virus, blueberry shock virus standard strains and variants thereof, tomato fruit ring spot virus, blueberry necrotic mottle virus and apple mosaic virus, solves the problem that the variants and different virus strains cannot be detected by a traditional ELISA method, covers main blueberry viruses and trans-boundary infected key virus types, and is high in specificity and high in sensitivity. The sensitivity is high.

The invention discloses a real-time fluorescent quantitative PCR (Polymerase Chain Reaction) method for detecting capsicum vein mottle virus in tobacco, which comprises the following steps of: firstly, designing and synthesizing a specific primer pair based on a coat protein CP gene conserved sequence of capsicum vein mottle virus isolate in an NCBI (National Center of Biotechnology Information) database; secondly, extracting total RNA of a tobacco sample infected with the pepper vein mottle virus, and performing reverse transcription to obtain cDNA; carrying out real-time fluorescent quantitative PCR (Polymerase Chain Reaction) amplification by using the obtained cDNA as a template and adopting the specific primer pair; and finally, judging whether the sample carries the target virus or not according to the amplification curve, the melting curve and the Ct value, and realizing quantitative analysis of the virus load through the standard curve. The method is high in specificity and sensitivity, compared with a conventional PCR method, the sensitivity is improved by 10 times, and rapid and accurate detection of the pepper vein mottle virus in the tobacco can be achieved.

The invention discloses a method for detecting a maize chlorotic mottle virus based on EmDEA. The primer probe composition capable of accurately detecting the maize chlorotic mottle virus is obtained aiming at a genome sequence of the maize chlorotic mottle virus on the basis of an EmDEA technology, and comprises a pair of DNA (Deoxyribonucleic Acid) primer pair, an RNA (Ribonucleic Acid) probe and twenty RT (Reverse Transcription) primers. By utilizing the primer probe composition, the invention establishes a method for detecting the maize chlorotic mottle virus based on the EmDEA. The method can be used for rapidly detecting the corn chlorotic mottle virus in plant material samples such as grains, the detection specificity is good, the sensitivity is high, the detection limit is only 100 copies / reaction, the operation is simple, the detection can be completed by reacting for 30 minutes at the constant temperature of 42 DEG C, and the detection time is short. The method is beneficial to quarantine of the maize chlorotic mottle virus and prevents spreading of the maize chlorotic mottle virus.

The invention discloses a wheat yellow mosaic disease unmanned aerial vehicle remote sensing monitoring method and system based on an RGB image, and relates to the field of intelligent agriculture, and the method comprises the steps: collecting RGB image data through an unmanned aerial vehicle for a breeding group naturally infected with wheat yellow mosaic disease, extracting a color index associated with the wheat yellow mosaic disease, and constructing a data set; a correlation analysis method is adopted, feature extraction is carried out through the data set, modeling is carried out through a machine learning algorithm, model parameter optimization is carried out through an optimization algorithm, and then a monitoring model is constructed and used for recognizing the wheat yellow mosaic disease through the unmanned aerial vehicle remote sensing technology. Technical support is provided for guaranteeing safe production of wheat and promoting development of intelligent breeding.

The invention provides a primer group, a reagent, a kit and a method for simultaneously detecting four pepper viruses and application of the primer group, the reagent, the kit and the method, and belongs to the technical field of gene detection. The primer group comprises a primer group 1 for detecting tomato spotted wilf virus (TSWV), a primer group 2 for detecting tobacco embrittlement virus (TRV), a primer group 3 for detecting pepper light mottle virus (PMMoV) and a primer group 4 for detecting potato virus X (PVX). The primer group provided by the invention is high in specificity, TSWV, TRV, PMMoV and PVX are simultaneously detected by utilizing the primer group provided by the invention, whether a to-be-detected sample is infected with TSWV, TRV, PMMoV or PVX viruses or not can be determined through one-time amplification detection, and the detection accuracy is improved on the basis of saving the detection time and reducing the detection cost.

The invention provides a method for rapidly detecting watermelon silver mottle virus in plants through RT-RPA and application of the method in disease diagnosis. By utilizing the method, a watermelon silver mottle virus sample can be quickly identified, and then a targeted prevention and treatment strategy is adopted to reduce the loss caused by diseases.

This invention proposes a single-tube detection reagent for eight target nucleic acids, primer and probe compositions, and their applications, belonging to the field of genetic engineering technology. The eight-target nucleic acid detection kit (fluorescent PCR method) for influenza A virus, influenza B virus, novel coronavirus N gene, novel coronavirus O gene, norovirus GI type, norovirus GII type, respiratory syncytial virus, and capsicum mottle virus described in this invention can simultaneously detect eight pathogen targets: influenza A virus, influenza B virus, novel coronavirus N gene, novel coronavirus O gene, norovirus GI type, norovirus GII type, respiratory syncytial virus, and capsicum mottle virus. Parallel detection of eight pathogen targets can be achieved through a single-tube reaction, reducing the time wasted due to incorrect reagent selection caused by target misidentification, and reducing the consumption of consumables and instrument space required by existing multi-tube reactions. This provides efficient detection technology support for the rapid identification of pathogens in wastewater or the environment.

‘HUTAAL29’ is a new and distinctive Alocasia plant which is characterized by foliage that exhibits an irregular variegation pattern with colors ranging from predominantly dark green with some amount of cream white to light yellow blotches to predominantly cream white to light yellow with dark green blotches, sporadic epidermal blistering on the adaxial foliar surface, pink foliar veins on the cream white to light yellow portions of the lamina, petioles that are greyed-red to greyed-purple when a large portion of the laminar surface is colored cream white to light yellow, and the stability of all characteristics from generation to generation.

Cucumber plants (Cucumis sativus) exhibiting increased resistance to Cumber Green Mottle Mosaic Virus (CGMMV) are provided, together with methods of producing, identifying, or selecting plants or germplasm with a CGMMV resistance phenotype. Such plants include cucumber plants comprising recombinant chromosomal segments conferring CGMMV resistance. Compositions, including novel polymorphic markers for detecting plants comprising introgressed virus resistance alleles, are further provided.

The invention discloses an application of Acinetobacter piceli LGC1-1 (Acinetobacter piceli sp.LGC1-1) in prevention and treatment of tobacco mosaic virus viruses, the Acinetobacter piceli LGC1-1 (Acinetobacter piceli sp.LGC1-1) is preserved in the China Center for Type Culture Collection on November 13, 2024, and the preservation number is CCTCC NO: M 20242552. The Acinetobacter piceli LGC1-1 is preserved in the China Center for Type Culture Collection on November 13, 2024, and the preservation number is CCTCC NO: M 20242552. The acinetobacter piceli LGC1-1 is used as an antagonistic strain and is used for inhibiting tobacco mosaic virus virus infection. The inhibition rates of the acinetobacter piceli LGC1-1 on the tobacco mosaic virus TMV and the pepper light mottle virus PMMoV are 82.8% and 77.7% respectively, while the inhibition rates of the conventional medicament ningnanmycin on the tobacco mosaic virus TMV and the pepper light mottle virus PMMoV are 50.8% and 53.3% respectively, which indicates that the acinetobacter piceli LGC1-1 has an outstanding passivation effect on the tobacco mosaic virus TMV and the pepper light mottle virus PMMoV; the control effect of the acinetobacter pichi LGC1-1 is far higher than that of a conventional medicament ningnanmycin, and the acinetobacter pichi LGC1-1 is a screened natural virus inoculated bacterium, has no pollution to the environment and does not destroy biological diversity.

The primer probe combination, kit and application for simultaneously detecting four corn pathogens belong to the technical field of molecular biology detection, and can solve the technical problems that the existing molecular biology detection methods for corn diseases cannot simultaneously detect multiple corn pathogens, or cannot simultaneously detect bacterial and viral pathogens of corn, and are complicated in operation and time-consuming. The primer probe combination for detecting corn inner state wilt fungus, corn bacterial wilt fungus, corn chlorotic mottle virus and corn dwarf mosaic virus, an integrated microfluidic chip kit containing the primer probe combination and a detection method are provided. The method for detecting pathogens in corn crops by using the primer probe combination and the kit has high sensitivity and accuracy, is simple, fast and time-saving in operation. The primer probe combination, kit and method can simultaneously detect the four pathogens, and play an important role in the on-site rapid detection of pathogens in corn planting.

The present invention discloses an Acinetobacter pekinensis LGC1-1 and its application in preventing and treating pepper vein mottle virus. The Acinetobacter pekinensis LGC1-1 (Acinetobacter sp.LGC1-1) has been deposited in the China Center for Type Culture Collection on November 13, 2024, with a deposit number of CCTCC NO: M 20242552. The inhibition rate of the Acinetobacter pekinensis LGC1-1 on pepper vein mottle virus ChiVMV is as high as 85.9%, while the inhibition rate of the conventional agent Ningnanmycin on pepper vein mottle virus ChiVMV is only 48.6%, indicating that the passivation effect of Acinetobacter pekinensis LGC1-1 on pepper vein mottle virus ChiVMV is outstanding, which is much higher than the prevention effect of the conventional agent Ningnanmycin, and Acinetobacter pekinensis LGC1-1 is a natural virus that has been screened out for antibacterial treatment, is environmentally friendly, and does not destroy biodiversity.

The invention discloses a mutant plasmid capable of resisting cucumber mosaic virus, tobacco vein banding mosaic virus and pepper vein mottle mosaic virus and application of the mutant plasmid, and belongs to the technical field of plant virology and molecular biology. The mutant plasmid contains a mutant RNA2 of a CMVFny isolate, and the nucleotide sequence of the mutant RNA2 is as shown in SEQ ID NO. 1. According to the invention, a plasmid vector pCCFR2-2bPT II with early termination of CMV 2b protein is used as a basic vector, and 100 bp fragments of tobacco vein banding mosaic virus and capsicum vein mottle mosaic virus are inserted into the basic vector to construct a mutant plasmid capable of resisting cucumber mosaic virus, tobacco vein banding mosaic virus and capsicum vein mottle mosaic virus. And a foundation is laid for prevention and treatment of composite infection of the three viruses.

The invention discloses application of a tobacco NbASL gene in improvement of plant virus resistance, and belongs to the technical field of molecular biology. The nucleotide sequence of the NbASL gene is as shown in SEQ ID NO: 1. The NbASL gene is silenced in nicotiana benthamiana, the sensitivity of the plant to potato Y virus (PVY) and pepper vein mottle virus (ChiVMV) is obviously enhanced, and the virus accumulation amount is obviously increased. On the contrary, overexpression of the NbASL gene can significantly inhibit infection of PVY and reduce accumulation of virus RNA. And the expression of the NbASL gene in the nicotiana benthamiana can be induced to be up-regulated by PVY or ChiVMV infection. Therefore, the NbASL gene is a positive regulatory factor in antiviral reaction of plants. The invention provides important gene resources and theoretical basis for cultivating broad-spectrum and durable antiviral new crop varieties by using the NbASL gene, and has important application value.

Cucumber plants (Cucumis sativus) exhibiting increased resistance to Cumber Green Mottle Mosaic Virus (CGMMV) are provided, together with methods of producing, identifying, or selecting plants or germplasm with a CGMMV resistance phenotype. Such plants include cucumber plants comprising recombinant chromosomal segments conferring CGMMV resistance. Compositions, including novel polymorphic markers for detecting plants comprising introgressed virus resistance alleles, are further provided.

This invention relates to the field of ecological cultivation technology of Chinese medicinal materials, specifically to a comprehensive prevention and control method for iron deficiency in *Polygonatum sibiricum* under forest cover. This invention addresses the symptoms of iron deficiency in *Polygonatum sibiricum* under forest cover by adjusting soil pH, providing effective iron supply, and combining a compound microbial agent of *Bacillus subtilis*, *Bacillus pumilus*, *Bacillus tekirae*, *Bacillus mycoides*, and *Bacillus amyloliquefaciens* with *Pseudomonas fluorescens* to release iron carriers. This fundamentally solves the problems of chlorosis and yellowish-white discoloration in young leaves, oily yellowish-brown spots on leaves, wilting of young shoots, elongated internodes, and easy lodging. Using *Polygonatum sibiricum* as the material, this invention proposes a three-step comprehensive approach to eliminate deficiency symptoms caused by iron deficiency during its ecological cultivation in forests. This allows the chloroplast thylakoid membrane and chlorophyll synthesis in *Polygonatum sibiricum* plants under forest cover to recover, photosynthesis to return to normal, and chlorophyll fluorescence kinetic parameters to a healthy state.

The invention discloses a cowpea light mottle virus isolate CpMMV as well as an infectious clone construction method and application. The whole genome sequence of the cowpea light mottle virus isolate CpMMV is as shown in SEQ ID NO: 1. The method comprises the following steps: constructing a whole genome sequence of the CpMMV on a pCambia1300 vector, so as to obtain a pCambia1300-CpMMV recombinant plasmid; and introducing into agrobacterium GV3101, so that the obtained agrobacterium can successfully infect cowpea and eggplant plants. The cowpea light mottle virus isolate CpMMV and the recombinant vector pCambia1300-CpMMV of the cowpea light mottle virus isolate CpMMV have the following characteristics: 1) the cowpea light mottle virus isolate CpMMV has infectivity and can successfully infect cowpea plants; 2) obvious virus symptoms can be caused on cowpea plants, and virus RNA is accumulated; 3) the method can be used for researching virus gene functions; and 4) as a virus source, the method can be used for screening antiviral cowpea varieties.

This invention belongs to the field of molecular detection technology for crop viruses, and discloses a kit and method for detecting three viruses in cucumber based on droplet digital PCR. This invention provides a composition for the simultaneous detection of multiple viruses in cucumber, namely, Chili Vein Mottle Virus (ChiVMV), Cucumber Green Mottle Mosaic Virus (CGGMV), and Watermelon Silver Mottle Virus (WSMoV). The composition includes specific primer pairs and fluorescent probes designed for multiple viruses, with sequences shown in SEQ ID NO:1-9. The technical solution of this invention achieves a detection sensitivity of 1 copy / μL, a repeatability coefficient of variation of <5%, and an overall detection cycle of only 6-8 hours. It can effectively detect low concentrations of latent infecting viruses, solving the technical problems of insufficient sensitivity, inability to simultaneously detect multiple target viruses, and inaccurate quantification in existing detection methods. This provides reliable technical support for early warning, precise prevention and control, and quarantine inspection of cucumber viral diseases.