28 results about "Mottle" patented technology

The invention relates to a primer group and a kit for detecting watermelon silver mottle virus and application of the primer group and the kit. The primer group is a specific primer aiming at the NSs protein gene of the watermelon silver mottle virus. The detection method is a fluorescent quantitative PCR (Polymerase Chain Reaction) detection method based on an SYBR Green dye method. By applying the primer group, the kit and the detection method, the genome of the watermelon silver mottle virus can be efficiently and accurately quantitatively detected. Whether a sample contains the watermelon silver mottle virus or not can be rapidly and accurately judged through detection, and the specific content level of the watermelon silver mottle virus is determined; when the kit is applied to detection, the infection state and information of the watermelon silver mottle virus on the plant can be more accurately obtained, so that prevention and control can be carried out in time. The method has the advantages of simplicity in result judgment, convenience and rapidness in operation, high specificity and sensitivity, wide application prospect and the like.

A new and distinct Alocasia plant named ‘ELSTLALU’ particularly distinguished by a dark green background leaf coloration with irregular white to light green mottling and marbling, distinct mottled and marbled variegation distributed across most of the leaf surface, and a tall, upright growth habit with elongated petioles and an open appearance.

The invention belongs to the technical field of crop disease prevention and control, and particularly relates to a planting method for preventing cucumber fruit blotch germs and green mottle viruses, which comprises the following steps: acquiring soil and crop historical pathogen data, environment monitoring data and cucumber seed batch information of a planting area; querying supply chain quarantine records of the seeds, and carrying out standardization treatment on the seeds; after planting, the disease probability of each growth stage is predicted through a risk assessment algorithm, a dynamic monitoring plan is generated in combination with a fuzzy mapping algorithm, plant samples are collected, PCR detection of melon bacterial fruit blotch and RT-PCR detection of cucumber green mottle mosaic virus are carried out, and a quarantine report is issued. Based on the monitoring plan, the quarantine report and the real-time environment data, judging a comprehensive risk level, triggering a corresponding field intervention scheme, and recording an execution result to form an intervention log; after planting is finished, a prevention and control efficiency report is generated. Therefore, the problems of insufficient disease detection, lack of intervention measures and the like in the prior art are solved.

The present invention relates to a benzoxazole derivative containing a dithioacetal unit, its preparation method, and use. The compound of the present invention exhibits excellent antiviral activity against cucumber green mottle mosaic virus and other viruses, and can be used as a pharmaceutical or medicament for preventing and treating plant viral diseases such as cucumber green mottle mosaic. Its structure and preparation process are simple, and its production cost is low. The benzoxazole derivative containing a dithioacetal unit includes a benzoxazole compound containing a dithioacetal unit, or a stereoisomer thereof, or a salt or solvate thereof. The structural formula of the benzoxazole compound containing a dithioacetal unit is as follows: #imgabs0#

The invention relates to a method for monitoring the richness and morbidity of litchi pocking spot disease infection sources, and provides a simple and accurate litchi pocking spot disease prediction method for monitoring and early warning of litchi pocking spot disease latent infection sources in litchi producing areas of different areas. According to the method, the separation frequency of pathogenic bacteria of the asymptomatic fruit pocking spot disease and the severity of the field pocking spot disease are collected and detected regularly (one week) in the fruiting period, and the method can accurately monitor the richness of latent infection sources of the pocking spot disease. On the basis, the occurrence condition of the litchi pocking spot disease in the region in the current year can be predicted by combining weather forecast (rainfall days) of the local litchi fruit bearing period.

‘HUTAPD75’ is a distinctive Philodendron plant which is characterized by uniquely variegated laminas with a green base coloration, light yellow to golden yellow flecking, and irregular blotches of varying size and color, from near-white to yellow, as well as green petioles and petiole wings with a combination of near-white and light yellow irregular longitudinal stripes, and the stability of all characteristics from generation to generation.

The invention belongs to the technical field of plant virus molecular detection, particularly discloses a multiplex RT-PCR method and a kit for detecting important blueberry viruses, and particularly provides a multiplex RT-PCR detection primer for the blueberry viruses, and the sequence of the primer is shown as SEQ ID NO.1-12. The technical scheme provided by the invention is wide in detection range, high in specificity, high in specificity and high in sensitivity. The kit can be used for simultaneously detecting blueberry scorching virus, blueberry shock virus standard strains and variants thereof, tomato fruit ring spot virus, blueberry necrotic mottle virus and apple mosaic virus, solves the problem that the variants and different virus strains cannot be detected by a traditional ELISA method, covers main blueberry viruses and trans-boundary infected key virus types, and is high in specificity and high in sensitivity. The sensitivity is high.

The invention discloses a real-time fluorescent quantitative PCR (Polymerase Chain Reaction) method for detecting capsicum vein mottle virus in tobacco, which comprises the following steps of: firstly, designing and synthesizing a specific primer pair based on a coat protein CP gene conserved sequence of capsicum vein mottle virus isolate in an NCBI (National Center of Biotechnology Information) database; secondly, extracting total RNA of a tobacco sample infected with the pepper vein mottle virus, and performing reverse transcription to obtain cDNA; carrying out real-time fluorescent quantitative PCR (Polymerase Chain Reaction) amplification by using the obtained cDNA as a template and adopting the specific primer pair; and finally, judging whether the sample carries the target virus or not according to the amplification curve, the melting curve and the Ct value, and realizing quantitative analysis of the virus load through the standard curve. The method is high in specificity and sensitivity, compared with a conventional PCR method, the sensitivity is improved by 10 times, and rapid and accurate detection of the pepper vein mottle virus in the tobacco can be achieved.

The invention discloses a method for detecting a maize chlorotic mottle virus based on EmDEA. The primer probe composition capable of accurately detecting the maize chlorotic mottle virus is obtained aiming at a genome sequence of the maize chlorotic mottle virus on the basis of an EmDEA technology, and comprises a pair of DNA (Deoxyribonucleic Acid) primer pair, an RNA (Ribonucleic Acid) probe and twenty RT (Reverse Transcription) primers. By utilizing the primer probe composition, the invention establishes a method for detecting the maize chlorotic mottle virus based on the EmDEA. The method can be used for rapidly detecting the corn chlorotic mottle virus in plant material samples such as grains, the detection specificity is good, the sensitivity is high, the detection limit is only 100 copies / reaction, the operation is simple, the detection can be completed by reacting for 30 minutes at the constant temperature of 42 DEG C, and the detection time is short. The method is beneficial to quarantine of the maize chlorotic mottle virus and prevents spreading of the maize chlorotic mottle virus.

The invention discloses a wheat yellow mosaic disease unmanned aerial vehicle remote sensing monitoring method and system based on an RGB image, and relates to the field of intelligent agriculture, and the method comprises the steps: collecting RGB image data through an unmanned aerial vehicle for a breeding group naturally infected with wheat yellow mosaic disease, extracting a color index associated with the wheat yellow mosaic disease, and constructing a data set; a correlation analysis method is adopted, feature extraction is carried out through the data set, modeling is carried out through a machine learning algorithm, model parameter optimization is carried out through an optimization algorithm, and then a monitoring model is constructed and used for recognizing the wheat yellow mosaic disease through the unmanned aerial vehicle remote sensing technology. Technical support is provided for guaranteeing safe production of wheat and promoting development of intelligent breeding.

The invention provides a primer group, a reagent, a kit and a method for simultaneously detecting four pepper viruses and application of the primer group, the reagent, the kit and the method, and belongs to the technical field of gene detection. The primer group comprises a primer group 1 for detecting tomato spotted wilf virus (TSWV), a primer group 2 for detecting tobacco embrittlement virus (TRV), a primer group 3 for detecting pepper light mottle virus (PMMoV) and a primer group 4 for detecting potato virus X (PVX). The primer group provided by the invention is high in specificity, TSWV, TRV, PMMoV and PVX are simultaneously detected by utilizing the primer group provided by the invention, whether a to-be-detected sample is infected with TSWV, TRV, PMMoV or PVX viruses or not can be determined through one-time amplification detection, and the detection accuracy is improved on the basis of saving the detection time and reducing the detection cost.

The invention provides a method for rapidly detecting watermelon silver mottle virus in plants through RT-RPA and application of the method in disease diagnosis. By utilizing the method, a watermelon silver mottle virus sample can be quickly identified, and then a targeted prevention and treatment strategy is adopted to reduce the loss caused by diseases.

This invention proposes a single-tube detection reagent for eight target nucleic acids, primer and probe compositions, and their applications, belonging to the field of genetic engineering technology. The eight-target nucleic acid detection kit (fluorescent PCR method) for influenza A virus, influenza B virus, novel coronavirus N gene, novel coronavirus O gene, norovirus GI type, norovirus GII type, respiratory syncytial virus, and capsicum mottle virus described in this invention can simultaneously detect eight pathogen targets: influenza A virus, influenza B virus, novel coronavirus N gene, novel coronavirus O gene, norovirus GI type, norovirus GII type, respiratory syncytial virus, and capsicum mottle virus. Parallel detection of eight pathogen targets can be achieved through a single-tube reaction, reducing the time wasted due to incorrect reagent selection caused by target misidentification, and reducing the consumption of consumables and instrument space required by existing multi-tube reactions. This provides efficient detection technology support for the rapid identification of pathogens in wastewater or the environment.

‘HUTAAL29’ is a new and distinctive Alocasia plant which is characterized by foliage that exhibits an irregular variegation pattern with colors ranging from predominantly dark green with some amount of cream white to light yellow blotches to predominantly cream white to light yellow with dark green blotches, sporadic epidermal blistering on the adaxial foliar surface, pink foliar veins on the cream white to light yellow portions of the lamina, petioles that are greyed-red to greyed-purple when a large portion of the laminar surface is colored cream white to light yellow, and the stability of all characteristics from generation to generation.

Cucumber plants (Cucumis sativus) exhibiting increased resistance to Cumber Green Mottle Mosaic Virus (CGMMV) are provided, together with methods of producing, identifying, or selecting plants or germplasm with a CGMMV resistance phenotype. Such plants include cucumber plants comprising recombinant chromosomal segments conferring CGMMV resistance. Compositions, including novel polymorphic markers for detecting plants comprising introgressed virus resistance alleles, are further provided.

The primer probe combination, kit and application for simultaneously detecting four corn pathogens belong to the technical field of molecular biology detection, and can solve the technical problems that the existing molecular biology detection methods for corn diseases cannot simultaneously detect multiple corn pathogens, or cannot simultaneously detect bacterial and viral pathogens of corn, and are complicated in operation and time-consuming. The primer probe combination for detecting corn inner state wilt fungus, corn bacterial wilt fungus, corn chlorotic mottle virus and corn dwarf mosaic virus, an integrated microfluidic chip kit containing the primer probe combination and a detection method are provided. The method for detecting pathogens in corn crops by using the primer probe combination and the kit has high sensitivity and accuracy, is simple, fast and time-saving in operation. The primer probe combination, kit and method can simultaneously detect the four pathogens, and play an important role in the on-site rapid detection of pathogens in corn planting.

The present invention discloses an Acinetobacter pekinensis LGC1-1 and its application in preventing and treating pepper vein mottle virus. The Acinetobacter pekinensis LGC1-1 (Acinetobacter sp.LGC1-1) has been deposited in the China Center for Type Culture Collection on November 13, 2024, with a deposit number of CCTCC NO: M 20242552. The inhibition rate of the Acinetobacter pekinensis LGC1-1 on pepper vein mottle virus ChiVMV is as high as 85.9%, while the inhibition rate of the conventional agent Ningnanmycin on pepper vein mottle virus ChiVMV is only 48.6%, indicating that the passivation effect of Acinetobacter pekinensis LGC1-1 on pepper vein mottle virus ChiVMV is outstanding, which is much higher than the prevention effect of the conventional agent Ningnanmycin, and Acinetobacter pekinensis LGC1-1 is a natural virus that has been screened out for antibacterial treatment, is environmentally friendly, and does not destroy biodiversity.

The invention discloses a mutant plasmid capable of resisting cucumber mosaic virus, tobacco vein banding mosaic virus and pepper vein mottle mosaic virus and application of the mutant plasmid, and belongs to the technical field of plant virology and molecular biology. The mutant plasmid contains a mutant RNA2 of a CMVFny isolate, and the nucleotide sequence of the mutant RNA2 is as shown in SEQ ID NO. 1. According to the invention, a plasmid vector pCCFR2-2bPT II with early termination of CMV 2b protein is used as a basic vector, and 100 bp fragments of tobacco vein banding mosaic virus and capsicum vein mottle mosaic virus are inserted into the basic vector to construct a mutant plasmid capable of resisting cucumber mosaic virus, tobacco vein banding mosaic virus and capsicum vein mottle mosaic virus. And a foundation is laid for prevention and treatment of composite infection of the three viruses.

The invention discloses application of a tobacco NbASL gene in improvement of plant virus resistance, and belongs to the technical field of molecular biology. The nucleotide sequence of the NbASL gene is as shown in SEQ ID NO: 1. The NbASL gene is silenced in nicotiana benthamiana, the sensitivity of the plant to potato Y virus (PVY) and pepper vein mottle virus (ChiVMV) is obviously enhanced, and the virus accumulation amount is obviously increased. On the contrary, overexpression of the NbASL gene can significantly inhibit infection of PVY and reduce accumulation of virus RNA. And the expression of the NbASL gene in the nicotiana benthamiana can be induced to be up-regulated by PVY or ChiVMV infection. Therefore, the NbASL gene is a positive regulatory factor in antiviral reaction of plants. The invention provides important gene resources and theoretical basis for cultivating broad-spectrum and durable antiviral new crop varieties by using the NbASL gene, and has important application value.

Cucumber plants (Cucumis sativus) exhibiting increased resistance to Cumber Green Mottle Mosaic Virus (CGMMV) are provided, together with methods of producing, identifying, or selecting plants or germplasm with a CGMMV resistance phenotype. Such plants include cucumber plants comprising recombinant chromosomal segments conferring CGMMV resistance. Compositions, including novel polymorphic markers for detecting plants comprising introgressed virus resistance alleles, are further provided.

This invention relates to the field of ecological cultivation technology of Chinese medicinal materials, specifically to a comprehensive prevention and control method for iron deficiency in *Polygonatum sibiricum* under forest cover. This invention addresses the symptoms of iron deficiency in *Polygonatum sibiricum* under forest cover by adjusting soil pH, providing effective iron supply, and combining a compound microbial agent of *Bacillus subtilis*, *Bacillus pumilus*, *Bacillus tekirae*, *Bacillus mycoides*, and *Bacillus amyloliquefaciens* with *Pseudomonas fluorescens* to release iron carriers. This fundamentally solves the problems of chlorosis and yellowish-white discoloration in young leaves, oily yellowish-brown spots on leaves, wilting of young shoots, elongated internodes, and easy lodging. Using *Polygonatum sibiricum* as the material, this invention proposes a three-step comprehensive approach to eliminate deficiency symptoms caused by iron deficiency during its ecological cultivation in forests. This allows the chloroplast thylakoid membrane and chlorophyll synthesis in *Polygonatum sibiricum* plants under forest cover to recover, photosynthesis to return to normal, and chlorophyll fluorescence kinetic parameters to a healthy state.

The invention discloses a cowpea light mottle virus isolate CpMMV as well as an infectious clone construction method and application. The whole genome sequence of the cowpea light mottle virus isolate CpMMV is as shown in SEQ ID NO: 1. The method comprises the following steps: constructing a whole genome sequence of the CpMMV on a pCambia1300 vector, so as to obtain a pCambia1300-CpMMV recombinant plasmid; and introducing into agrobacterium GV3101, so that the obtained agrobacterium can successfully infect cowpea and eggplant plants. The cowpea light mottle virus isolate CpMMV and the recombinant vector pCambia1300-CpMMV of the cowpea light mottle virus isolate CpMMV have the following characteristics: 1) the cowpea light mottle virus isolate CpMMV has infectivity and can successfully infect cowpea plants; 2) obvious virus symptoms can be caused on cowpea plants, and virus RNA is accumulated; 3) the method can be used for researching virus gene functions; and 4) as a virus source, the method can be used for screening antiviral cowpea varieties.

This invention belongs to the field of molecular detection technology for crop viruses, and discloses a kit and method for detecting three viruses in cucumber based on droplet digital PCR. This invention provides a composition for the simultaneous detection of multiple viruses in cucumber, namely, Chili Vein Mottle Virus (ChiVMV), Cucumber Green Mottle Mosaic Virus (CGGMV), and Watermelon Silver Mottle Virus (WSMoV). The composition includes specific primer pairs and fluorescent probes designed for multiple viruses, with sequences shown in SEQ ID NO:1-9. The technical solution of this invention achieves a detection sensitivity of 1 copy / μL, a repeatability coefficient of variation of <5%, and an overall detection cycle of only 6-8 hours. It can effectively detect low concentrations of latent infecting viruses, solving the technical problems of insufficient sensitivity, inability to simultaneously detect multiple target viruses, and inaccurate quantification in existing detection methods. This provides reliable technical support for early warning, precise prevention and control, and quarantine inspection of cucumber viral diseases.

The invention belongs to the technical field of rapid diagnosis of plant viruses, and particularly relates to an innovative application based on a loop-mediated isothermal amplification (LAMP) technology, in particular to a rapid detection method aiming at an atractylodes lancea light mottle virus. The invention discloses a specific primer combination specially used for detecting a light mottle virus of rhizoma atractylodis. The specific primer combination comprises an outer primer pair, an inner primer pair and two loop primers. Meanwhile, the invention also provides an efficient and convenient detection process which comprises the following steps: setting a reaction system by using the primers, and amplifying for 30 minutes at the constant temperature of 63 DEG C; if the color of the reaction liquid is kept in the yellowish purple, judging that the reaction liquid is a negative result; if the color is turned into light blue, the sample is positive. The method has the advantages of being high in sensitivity, easy and convenient to operate, rapid in reaction and the like, and can be widely applied to on-site rapid screening and diagnosis of the atractylodes lancea light mottle virus.

This invention provides a method for constructing spectral indices for monitoring fava bean red spot disease. First, spectral curves of different degrees of fava bean red spot susceptibility are obtained based on spectral reflectance, and the sensitive bands and red spot sensitivity indices for each spectral band are obtained. Then, based on... PROSAIL The model, combined with the erythrophyll sensitivity index of each spectral band, is used to obtain the canopy insensitive bands; finally, the erythrophyll index is constructed. LSDI This method constructs a spectral index that is sensitive to broad bean red spot disease but insensitive to canopy structure, based on the spectral characteristics of broad bean red spot disease and the canopy reflectance characteristics of healthy vegetation. This aims to enhance disease information while suppressing canopy structure difference information, thereby satisfying the requirement that a single spectral feature is applicable to the monitoring of red spot disease in different varieties of broad beans, and providing guidance for broad bean planting and production.

The invention discloses a primer probe combination for simultaneously detecting four maize pathogens, a kit and application thereof, and belongs to the technical field of molecular biological detection. In order to solve the technical problems that the existing molecular biological detection method for corn diseases cannot simultaneously detect various corn pathogens or cannot simultaneously detect corn bacterial pathogens and virus pathogens, the operation is tedious, the consumed time is long, and the like, the invention provides a method for detecting corn diseases. The invention provides a primer probe combination for detecting Cmn, Cmn, maize chlorotic mottle virus and maize dwarf mosaic virus in maize crops, an integrated micro-fluidic chip kit containing the primer probe combination and a detection method. The method for detecting the pathogens in the corn crops by adopting the primer probe combination and the kit provided by the invention has relatively high sensitivity and accuracy, is simple and convenient to operate, and is rapid and time-saving. The primer probe combination, the kit and the method provided by the invention can detect the four pathogens at the same time, and play an important role in on-site rapid detection of the pathogens in corn planting.

The invention relates to the technical field of gene engineering, and provides a nucleic acid quality control sample containing a corn chlorotic mottle virus CP gene and a corn dwarf mosaic virus CP gene target sequence and a preparation method thereof. The standard substance adopts lentivirus particles as a carrier, and specific nucleic acid fragments of maize chlorotic mottle virus and maize dwarf mosaic virus are integrated into a lentivirus expression system to form a nucleic acid quality control sample with a virus-like structure. The nucleic acid standard substance is presented in the form of virus particles, a protein shell effectively wraps target RNA, a real virus structure is simulated, biological infectivity is avoided, and the whole process from nucleic acid extraction to detection can be monitored. Compared with the prior art, the standard substance overcomes the defect that a traditional RNA quality control substance is easy to degrade, can be stably stored for more than 3 months at normal temperature and can be stably stored for more than 12 months at 4 DEG C, and is suitable for long-distance transportation and clinical application.

The invention belongs to the field of molecular biology, and particularly relates to an SV marker closely linked with eggplant brown spot resistance and application. The SV marker is a 119 bp deletion sequence of Chr11: 98755912, and the nucleotide sequence of the SV marker is as shown in SEQ ID NO. 1; by developing the specific molecular marker of the SV98755912, a theoretical basis and an effective molecular marker can be provided for eggplant brown spot resistance molecular breeding.