337 results about "Mottle" patented technology

An apparatus and method for evaporating a coating solvent from a coating on a substrate and for minimizing the formation of mottle. The coating is heated with a first drying gas at no higher than a first heat transfer rate. The first heat transfer rate is created by a first heat transfer coefficient and a first temperature difference between the first coating temperature and the first drying gas temperature. The first heat transfer rate causes maximum evaporation of the coating solvent yet insignificant formation of mottle when the coating is at the first coating thickness and the first coating viscosity.

Apparatus for drying coated web material and preferably moving web material comprises a nozzle, means for supplying air to the nozzle and means to distribute the air through said nozzle substantially uniformly across the web width, said nozzle arcing from a position perpendicular with respect to the plane of the web to a position substantially parallel with respect to the plane of the web, said nozzle having an exit slot wherein the air is discharged from the exit slot at an angle of between 1 DEG and 45 DEG with respect to the plane of the web.

The artistic powder paint with mottle effect is prepared with resin and its curing system, deairing agent, flow assistant, pigment, stuffing, mottle agent, effect pigment, powder flow promoter, and through preparing base material with the first mentioned five materials, adding the other materials, dry mixing, and sieving. The artistic powder paint of the present invention has no toxicity, no pollution, high use effect and reasonable cost.

The invention provides a preparing and application method of diffusion medium box for diagnosing livestock's snail fever spot gold, which is a new immune labeling technique and livestock's snail fever immunology diagnosis technique. The invention labels colloid gold onto Japanese snail antigen protein, and is suit for testing many kinds of animals such as cattle, sheep, pig and rabbit; it spots tested animal's blood dried paper leachate onto pyroxylin film as posited phase, then it droplets snail antibody colloid gold 2í½3 drops (100í½150ª–l) and produces naked eye visible red speckle in 2 minutes. Its positive coincidence rate is 100% and its negative coincidence rate is 99.2%.

The invention relates to a high-efficiency and simple method for detecting a human papilloma virus (HPV) neutralizing antibody, which can be applicable to high-throughput detection of the human papilloma virus neutralizing antibody. The method is characterized by using a spot detection method for detecting cells infected by human papilloma virus or pseudotype virus. The invention further discloses applications of the method in screening and identifying the neutralized monoclonal antibody against HPV, evaluating the immunity protectiveness of HPV vaccine, the potency test (ED50 test) of HPV vaccine, etc.

The invention relates to preparation and application of a rickettsia discrimination detection gene chip. The preparation method comprises the following steps: preparing universal and specific primers, preparing seven rickettsia nucleic acid typing probes, preparing an oligonucleotide chip, establishing a multiplex PCR (polymerase chain reaction) system, and establishing a hybridization system. The gene chip can simultaneously discriminate seven important rickettsiae, including rickettsia prowazeki, rickettsia mooseri, spotted fever group rickettsia, Coxiella burnetii, Orientia tsutsugamushi, Ehrlichia chaffeensis and Anaplasma phagocytophilum. The gene chip has the advantages of high speed, high accuracy, high flux and high specificity, and can provide a novel detection means for clinical diagnosis and epidemiological investigation of rickettsia infection.

The invention relates to a method for rapidly detecting neutralizing antibody of enterovirus with high efficiency and a kit therefor. The method is suitable for high-throughput detection of the neutralizing antibody of the enterovirus, and is characterized by combining enzyme linked immune spot assay with a spot detecting instrument to detect cells infected by the enterovirus. The invention also discloses the usage of the method in the aspects such as neutralizing enterovirus monoclonal antibody, neutralizing titer for detecting the enterovirus monoclonal antibody, etc.

The invention relates to a kit for detecting a hepatitis c virus genotype, particularly relates to that the nucleic acid reverse dot blot hybridization technology is used to prepare a kit for hepatitis c virus genotype detection. The invention is used for rapidly and accurately distinguishing the hepatitis c virus genotype in a clinical blood sample.

The invention discloses an immunodetection kit specially used for rice black-streaked dwarf virus in single planthopper and a using method thereof. Specific polyclonal antibodies of the rice black-streaked dwarf virus (RBSDV) are utilized to build a dot immuno-binding assay (DIBA) detecting method which is used for building the detection kit for rice black-streaked dwarf virus, main reagents of the kit are working solution or concentrated solution, and the kit has the characteristics of convenient use, simple operation, and the like. And simultaneously, the use of the rice black-streaked dwarf virus detection kit can be a standard program or a reduced program, which can greatly broaden the using ranges and applicable persons of the kit and facilitate the population and application of the technique to establishment units, and further, the kit is applied in the fields of plant disease prognosis and prediction and the formulation of prevention and curing strategies, and the like. In addition, the kit can be used for measuring the inoculation strength in varietal resistance seed selection of rice black-streaked dwarf virus, and the like.

The invention provides a bactericidal composition containing tetramycin and zhongshengmycin. The bactericidal composition comprises compounded effective components consisting of tetramycin and zhongshengmycin, with the balance being auxiliary components. The mass ratio of tetramycin to zhongshengmycin in the composition is 1-50 50-1, and the mass fraction of tetramycin and zhongshengmycin in a preparation is 1 to 80%, with the balance being auxiliary components which are allowed to use and admitted in pesticides; dosage forms of the composition comprise missible oil, a suspending agent, a wettable powder, a water dispersible granule, an emulsion in water, a micro-emulsion, a granule and a micro capsule. The composition is mainly used for preventing and treating rot diseases of fruit trees, alternaria leaf spot, rice blast, soybean root rot, fusarium wilt of cucurbits, cotton verticillium wilt, rust diseases of jujube trees, white rot of grape, black spot of ginseng and pseudo-ginseng, Exobasidium vexans Massee of tea, forest rot, canker, gumming diseases, leaf cast, damping off of forest seedlings, soft rot bacteria of vegetable, Pseudomonas lachrymans, Xanthomonas oryzae pv. oryzae, Physalospora piricola, Fusarium graminearum, etc.

The invention relates to a reagent kit for diagnosing enzyme linked immune spots infected by tubercle bacillus and a method for preparing a specific antigen. The reagent kit comprises a reagent kit body and a detection reagent arranged in the kit body; the detection reagent comprises a positive standard solution, a chromogenic agent, a concentrated detergent and a diluent; the detection reagent also comprises a specific antigen of mycobacterium tuberculosis, a coated INF-gamma resistant antibody and an enzyme label secondary antibody of a vector protein conjugate; the specific antigen of mycobacterium tuberculosis is fusion protein expressed by an ESAT-6 gene and an EIS gene of the mycobacterium tuberculosis; and the INF-gamma resistant antibody is a murine IgG antibody. The method for preparing the specific antigen comprises the following steps: proper tubercle bacillus special antigen ESAT-6 and EIS are combined; and the prepared recombining fusion protein contains main antigenic determinants of two antigens. The recombining fusion protein definitely contains a T cell epitope suitable for the limitation of different HLA, and does not influence results by different groups and different HLA distribution. Clinical tests on tuberculosis patients and healthy people of different groups show that the reagent kit has more excellent specificity and sensitivity than a current similar product.

The invention discloses application of protein Rh054_01780 to rickettsia heilongjiangensis-resistant immune protection, and provides application of the protein Rh054_01780 to preparation of a vaccine for far eastern spotted fever, a vaccine for rickettsia heilongjiangensis, a protective antigen for far eastern spotted fever, or a protective antigen for rickettsia heilongjiangensis. The protein Rh054_01780 is (a) or (b), wherein (a) is a protein which has an amino acid sequence shown as a sequence 1 in a sequence table; and (b) is a protein which is formed by substituting and/or deleting and/or adding one or more amino acid residues in the amino acid sequence shown as the sequence 1 in the sequence table, has the same function and is derived from the sequence 1. Compared with a mycoproteinvaccine, a vaccine prepared from the protein Rh054_01780 has the advantages of simple and convenient preparation method, low cost, high yield, safety and the like; and the protein Rh054_01780 has significant value in the aspect of epidemic prevention and treatment of far eastern spotted fever.

A rapid and sensitive PCR-based assay is provided to facilitate early detection of Edwardsiella ictaluri in channel catfish. This bacteria is the causative agent in the disease, Enteric septicemia of catfish (ESC). Also provided is a method of selecting breeding stock for use in selective breeding programs to improve disease resistance in channel catfish. Also provided is a method of determining the efficacy of vaccines produced against ESC.

The invention discloses a microsatellite family identification method for ictalurus punctatus. The microsatellite family identification method comprises the following steps: (1) breeding an ictalurus punctatus family by adopting an artificial propagation method, and carrying out polyculture on 100 family descendants in one pond; (2) analyzing gene types of an ictalurus punctatus breeding group on 18 microsatellite marker sites by adopting a fluorescence marking primer and a multi-PCR (Polymerase Chain Reaction) amplification method; screening 10 effective microsatellite markers which have high polymorphism and are suitable for family identification; (3) optimizing a multi-PCR amplification system of the 10 microsatellite markers and analyzing gene types of the polycultured descendants on 10 microsatellite marker sites; (4) identifying a parental origin of each descendant according to the gene types of parents and the descendants. According to the microsatellite family identification method disclosed by the invention, a paternity testing method is established for the ictalurus punctatus for the first time and the identification accuracy reaches 96.6 percent; polyculture individual parents of the ictalurus punctatus can be rapidly and accurately identified. The microsatellite family identification method disclosed by the invention can be used for rapidly and accurately carrying out family identification on the polyculture individual parents of the ictalurus punctatus, and the breeding effect is improved.

A replenishment and image mottle reduction system for adding carrier particles to a developer housing in a two-component developer toner imaging machine. The replenishment system includes (i) a carrier-only hopper for receiving and containing a first quantity of carrier particles; (ii) metering valves connected to a discharge end of the carrier-only hopper; (iii) a pneumatic plenum connected to the metering valves; (v) an air blower connected to the carrier-only hopper and to the pneumatic plenum for pressurizing the carrier-only hopper and for pneumatically conveying a metered quantity of carrier particles in an air stream from the pneumatic plenum; and (vi) a carrier separator assembly connected to the pneumatic plenum, located above each developer housing and including a carrier current collector, for separating fresh carrier from the air stream, and for allowing the carrier separated as such to drop by gravity into the developer housing.

The invention discloses a method for detecting the titre of live viruses, which comprises the following steps: firstly, infecting diploid cells or other adherent cells with dilute viruses to be detected, culturing and fixing the infected cells; secondly, adding virus antiserum (first antibody) into the fixed cells, culturing and washing, adding a second antibody marked by horseradish peroxidase (HRP), culturing and washing, adding coloring solution for color development and producing red or brown spots, or adding virus antiserum marked by the HRP into the fixed cells, culturing the virus antiserum and washing, adding the coloring solution for color development and producing red or brown spots; thirdly, calculating the virus titre according to the quantity of the red or brown spots and the dilution multiple, or calculating the 1gTCID50 value of the viruses according to the dilution degree and the hole numbers of the spots. The detection method can be applicable to the titre detection of various live viruses, can shorten the detection time of the titre of the viruses due to fast detection speed, has good specificity as the immune spots are caused by special immune reaction, low detection cost as well as simple and convenient operation and does not need special instruments of optical or fluorescent microscopes, and the like or other reagents.

The invention relates to an application for extracting natural compound (I) from black mulberry, the compound (I) has strong inhibition effect for tyrosinase and is applied for preventing skin mottle, freckle, senile plaque or the like skin chromatosis and whitening, and the compound (I) is a food additive. The activity of the compound (I) is strong proved by the mensuration of the inhibition activity, the compound (I) has good stability and obvious growth inhibition action for the melanoma cell, can reduce the activity of the tyrosinase in the melanoma cell and biological synthesis level of the melanin in the cell.

The invention provides a prevention and treatment method of alternaria leaf spot of potted apple trees, and belongs to the field of fruit tree pest control. The prevention and treatment method comprises the following steps: 1, Chinese herbal medicine raw material pretreatment; 2, potted apple tree seedling root-soaking treatment; 3, cultivation soil preparation; 4, transplanting; and 5, watering. The prevention and treatment method has the beneficial technical effects that the occurrence of the alternaria leaf spot of the potted apple trees is greatly reduced, the pathogenic bacteria drug resistance degree of the alternaria leaf spot of the potted apple trees is reduced.

The invention discloses a bactericidal composition containing tetramycin and pyraclostrobin. The effective components of the pesticide composition are tetramycin and pyraclostrobin in binary compounding, and the rest components are auxiliary components, wherein the mass ratio of tetramycin to pyraclostrobin in the pesticide composition is (1-50):(50-1); tetramycin and pyraclostrobin as the effective components in the preparation account for 1-80% by mass, and the auxiliary components allowable and acceptable to pesticide account for the rest. The dosage forms of the pesticide composition disclosed by the invention include emulsifiable concentrate, suspending agent, wettable powder, water dispersible granules, emulsion in water and microemulsion. The pesticide composition realizes a specific effect on fungal diseases such as powdery mildew of cucumber, downy mildew, fruit tree rot and alternaria leaf spot, rice blast, soybean root rot, cucurbits fusarium wilt, cotton verticillium wilt, jujube rust, grape white rot, panax pseudoginseng black spot, tea blister blight, wood rot, canker, gummosis, leaf cast, damping off in seedling stage and the like.

The invention belongs to the technical field of biology, and particularly relates to a preparation method for aeromonas hydrophila outer membrane protein gene prokaryotic expression protein. The method comprises the following steps that the protein clones an outer membrane protein gene segment into a prokaryotic expression vector pET-30a to obtain a recombinant expression vector, the recombinant expression vector is converted into Escherichia coli to obtain recombinant Escherichia coli, the recombinant Escherichia coli is inoculated to an Amp-resistant LB plate to be cultured for OD600 for 0.6 h, IPTG is added to reach the final concentration being 1.0 mmol/l for inducible expression, and the aeromonas hydrophila outer membrane protein gene prokaryotic expression protein is obtained after purification is performed. According to the method, the aeromonas hydrophila outer membrane protein gene prokaryotic expression protein is successfully constructed, the aeromonas hydrophila outer membrane protein gene prokaryotic expression protein can be used as a vaccine capable of protecting fish, the immune effects of the aeromonas hydrophila outer membrane protein gene prokaryotic expression protein pompA for channel catfish under different FIA effects are compared and researched, and a foundation is laid for further researching the pompA and researching whether the pompA can be used as the candidate component of the genetic engineering subunit vaccine or not.