21 results about "Membrane integrity" patented technology

The invention discloses a PDRN-loaded umbilical cord mesenchymal stem cell source nano-vesicle and a preparation method thereof, and belongs to the technical field of biological medicines. The preparation method provided by the invention comprises the following steps: mixing an umbilical cord mesenchymal stem cell suspension with a PDRN solution, and carrying out freeze thawing treatment; incubating the mixed solution subjected to freeze thawing treatment; extruding the incubated mixed solution for multiple times by using a filter membrane; and collecting the extruded product, and separating and purifying to obtain the PDRN-loaded umbilical cord mesenchymal stem cell source nano-vesicle. According to the preparation method disclosed by the invention, the encapsulation efficiency of PDRN is remarkably improved, meanwhile, secondary damage of a traditional post-loading technology to a preformed vesicle structure is avoided, the membrane integrity and biological activity of a final product are better maintained, the yield is high, and the preparation process is rapid; the introduction of exogenous impurities is avoided from the source, and the high purity and biological safety of the obtained nano-vesicle component are ensured.

The invention discloses an immune agonist and a preparation method thereof, and relates to the technical field of immune agonist preparation, and the preparation method of the immune agonist comprises the following specific steps: S1, preparing a double-target immune agonist monomer; s2, preparing a cationic liposome inner core; s3, biomimetic cell membrane coating; and S4, finished product detection. According to the immune agonist and the preparation method thereof, in the preparation process, a continuous flow microreactor is used for two-step synthesis of a double-target monomer, a segmented precise temperature control mode is matched with microwave strengthening, the reaction period can be shortened, efficient entrapment of the agonist is achieved through a micro-reaction technology, and the problems that a traditional batch method is low in entrapment efficiency and uneven in particle size are solved; in addition, MDC cell membrane extraction is conducted through differential centrifugation, the integrity of the membrane is guaranteed, active targeting of a CD11c marker on the surface of the MDC cell membrane is achieved through a cell biomimetic membrane, and after lipidosome and membrane vesicles with the volume ratio being 3: 1 are fused, the in-vitro BMDC binding rate is increased compared with that of naked lipidosome.

This invention discloses a dual-inspection device and method for single membranes in a water treatment membrane module, relating to the field of water treatment technology. Each single membrane is equipped with an outlet solenoid valve. The main control box controls the opening and closing of the outlet solenoid valve to perform individual inspections and a binary inspection of the membrane module. The main control box identifies damaged single membranes based on the outlet turbidity value measured by a turbidity detector; it also identifies fouled single membranes based on the transmembrane pressure difference value measured by a pressure sensor, cleans the fouled single membranes, while normal membranes continue to produce water. Through individual inspection and binary inspection methods, as well as targeted membrane fouling cleaning, rapid inspection of membrane integrity and filtration performance can be achieved, and damaged and fouled membranes can be located. Then, corresponding measures such as targeted membrane fouling control can be taken, avoiding the occurrence of downtime for the entire membrane module due to problems with individual membranes. This greatly improves the water treatment efficiency of the membrane module and significantly reduces the energy consumption of the equipment.

The invention provides a polypeptide or a pharmaceutically acceptable salt thereof and application of the polypeptide or the pharmaceutically acceptable salt in preparation of a tumor multidrug resistance reversal agent, and belongs to the technical field of biological medicines. The polypeptide can selectively act on tumor cells with negatively charged cell membranes through electrostatic interaction, has small influence on normal cells, and has good selectivity; meanwhile, a hydrophobic fragment in the structure is inserted into a tumor cell membrane phospholipid skeleton to destroy the integrity of a cell membrane, change the permeability of the cell membrane and inhibit the membrane protein function, so that more anti-tumor drug molecules can be promoted to enter tumor cells, the accumulation of the drug in the tumor cells is improved, the function of ABC transport protein on the membrane can be influenced, and the anti-tumor effect is improved. The excretion of intracellular drugs is inhibited, and finally, the aim of reversing the multidrug resistance of tumors is fulfilled. Therefore, the polypeptide or the pharmaceutically acceptable salt thereof can be used as a novel peptide tumor multidrug resistance reversal agent, can be potentially used in anti-tumor clinical treatment, and has a wide development prospect.

This invention belongs to the field of animal husbandry technology and relates to a method for improving the quality of frozen semen from Lanzhou fat-tailed sheep based on ergothioneine. This application, through optimized combination of diluent, 8 layers of gauze wrapping for cooling, 4 cm fumigation height, and 8 mM ergothioneine, achieved a sperm motility rate of 45.80±3.62%, an acrosome integrity rate of 52.32±2.28%, and a plasma membrane integrity rate of 55.38±3.55% after thawing. All indicators are significantly better than those of the traditional single-optimization group.

The invention provides application of exogenous D-ribose in improving the normal-temperature preservation effect of boar semen and a method for improving the normal-temperature preservation effect of the boar semen. According to the technology disclosed by the invention, D-ribose is added into the boar semen stored at normal temperature, so that the effects of enhancing the oxidation resistance of the semen, improving the sperm motility and improving the quality of the semen can be achieved, and particularly, indexes such as the sperm motility, the plasma membrane integrity rate, T-AOC, CAT, GSH-Px and SOD are obviously higher than those of all groups including a control group; the ROS content of the sperms is obviously lower than that of all other groups.

This invention belongs to the field of veterinary drug technology. Specifically, it discloses a synergistic var. fluoraranil-moxicillin drop containing plant essential oils. The plant essential oils in the drop can disrupt the lipid structure of the parasite's epidermal wax layer, reduce the epidermal barrier function, increase epidermal permeability, and promote the rapid penetration of fluoraranil and moxicillin into the parasite, thereby increasing the drug concentration and shortening the onset time. At the same time, it also helps to enhance the effect of transdermal penetration enhancers. Fluoraranil acts on the parasite's GABA-gated chloride ion channels, moxicillin acts on the parasite's neuromuscular junction, and the plant essential oil exerts its insecticidal effect by disrupting the parasite's cell membrane integrity and inhibiting its metabolic enzyme activity. The three have different targets and no cross-resistance. They can synergistically block the key survival links of parasites, significantly improve the insecticidal rate, and delay the development of parasite resistance to single drugs.

This invention discloses a cryopreservation diluent containing fucoxanthin and its applications. Adding fucoxant to the cryopreservation diluent enhances the antioxidant capacity of frozen-thawed semen, reduces oxidative damage to goat sperm from reactive oxygen species during freezing-thawing, significantly improves sperm viability, motility, plasma membrane integrity, and total antioxidant capacity after freezing-thawing, enhances catalase and total superoxide dismutase activity, and reduces reactive oxygen species and malondialdehyde content. The effect is best when the fucoxant concentration is 2 μM during cryopreservation. The cryopreservation diluent of this invention is simple to prepare and operate, significantly improves the cryopreservation effect of semen, and has important practical application value.

The invention relates to the field of boar semen preservation, in particular to preparation and application of a ferulic acid-based boar semen low-temperature diluent. The proportion of the ferulic acid in the basic diluent is 0.2 to 0.8 mmol / L; wherein the basic diluent is prepared by dissolving 3.00 g of glucose, 4.00 g of cane sugar, 0.03 g of gentamicin sulfate and 20 mL of yolk in 80 mL of double distilled water, uniformly stirring, centrifuging at 4 DEG C and 7000 * g for 25 min, adjusting the pH value of the solution to 6.80-7.00, and adjusting the osmotic pressure of the solution to 290-300 mOsm / kg. The method has the beneficial effects that ferulic acid is used as a protective agent for preserving the boar semen at 4 DEG C for the first time, the effect is reliable, popularization is easy, the preserved boar semen is suitable for artificial insemination and artificial insemination after long-distance transportation, the motility, motility rate, acrosome integrity rate and plasma membrane integrity rate of boar sperms preserved at 4 DEG C can be remarkably improved, and the fertilization activity of the sperms is kept.

Cryopreservation of endothelial cell monolayers is one of the major challenges in the cryopreservation of complex tissues. Human umbilical vein endothelial cells (HUVECs) in suspension are available commercially and recently their post-thaw cell membrane integrity was significantly improved by cryopreservation in 5% dimethyl sulfoxide (DMSO) and 6% hydroxyethyl starch (HES). However, cryopreservation of cells in monolayers has been elusive. The exact mechanisms of damage during cell monolayer cryopreservation are still under investigation. Here, we show that a combination of different factors contribute to significant progress in cryopreservation of cell monolayers. The addition of 2% chondroitin sulfate to 5% DMSO and 6% HES and cooling at 0.2 or 1° C. / min led to high membrane integrity (97.3±3.2%) immediately after thaw when HUVECs were cultured on a substrate with a coefficient of thermal expansion similar to that of ice. The optimized cryopreservation protocol was applied to monolayers of primary porcine corneal endothelial cells, and resulted in high post-thaw viability (95.9±3.7% membrane integrity) with metabolic activity 12 hours post-thaw comparable to unfrozen control.

The invention discloses a diluent for normal-temperature preservation of boar semen and a preservation method. Selenomethionine is selected as a protective agent in the diluent for preserving the boar semen at the normal temperature to improve the semen quality, the diluent for preserving the semen at the low temperature is a Modena basic diluent, and the addition amount of the selenomethionine in the diluent for preserving the semen at the normal temperature is 0.1-10 mg / L, preferably 1 mg / L. According to the invention, selenomethionine is added into the diluent for preserving the boar semen at normal temperature, so that the motility, motility rate, plasma membrane integrity rate and acrosome integrity rate of the boar semen can be remarkably improved. If the method is applied to actual production, the normal-temperature preservation time of the boar semen can be prolonged, the semen preservation quality is improved, and the production cost is reduced.

The application discloses an antibacterial peptide Ajaponin of Japanese eel and application thereof. The antibacterial peptide Ajaponin is composed of 21 amino acids and has a molecular weight of 2693.29 Dalton. The antibacterial peptide Ajaponin contains 7 positively charged amino acid residues, has a hydrophobicity of 0.316 and an isoelectric point of 11.45. The antibacterial peptide Ajaponin is a novel cationic short peptide, has good water solubility, high antibacterial activity, low toxicity and other advantages, is composed of limited amino acid residues, has compact structure, is easy to synthesize and purify, and is convenient for large-scale production and application. In addition, the antibacterial peptide Ajaponin contains multiple positively charged amino acid residues, is beneficial to interaction with negative charges of bacterial cell membranes, destroys the membrane integrity, and thus plays a role in rapid bacteriostasis and sterilization.

The invention belongs to the technical field of antibacterial medicines, and particularly relates to a pyrazole functional group modified metal iridium complex as well as a preparation method and application thereof. The structural formula of the pyrazole functional group modified metal iridium complex is as shown in formula I in the specification. The pyrazole group is introduced into the ligand of the cyclometalated iridium complex, so that the capability of penetrating and destroying bacterial cell membranes is remarkably enhanced, and the antibacterial activity is improved. Tests prove that the metal iridium complex plays a bactericidal role by destroying membrane integrity, inducing cellular content leakage and depolarization of a membrane, can remarkably inhibit formation of golden yellow pigment and a biological membrane of staphylococcus aureus, is not easy to induce bacteria to generate drug resistance, and has a good application prospect in clinical anti-infection treatment. Formula I.

This invention relates to a method for the simultaneous removal of antibiotic-resistant bacteria and resistance genes using a solar-driven interfacial heat-Fenton oxidation-like process. The method involves placing a photothermal film loaded with carbon black composite photothermal material in a photothermal sterilization reactor, introducing antibiotic-resistant wastewater, adding H2O2, turning on a xenon lamp to preheat the photothermal film, sealing the reactor, and allowing the wastewater to pass through the photothermal film under gravity. This invention utilizes a photothermal conversion material to absorb light radiation and convert it into thermal radiation, treating antibiotic-resistant bacteria through the high-temperature interfacial heat effect generated on the photothermal film. High-temperature interfacial heat causes localized heating of the bacterial cell membrane structure, leading to a certain degree of breakage of fatty acid chains in the bacterial membrane lipid bilayer. This increases cell membrane permeability, and the resulting physical damage damages the bacterial outer membrane structure, further increasing membrane permeability and thus disrupting membrane integrity.

The invention relates to a controlled oxidation opening host-guest material of a targeted tumor cell membrane and an application thereof in selective ketonization of tumor phospholipids, and belongs to the field of biological materials and nano medicine. The material is formed by assembling a metal-organic framework material modified by a metal complex and a fluorescent probe targeting receptor tyrosine kinase, and selective oxidation of phospholipid targeting a tumor cell membrane is realized by utilizing the efficient and controllable oxidation catalytic performance of a subject-object. SPR can recognize and combine with RTK overexpressed on a tumor cell membrane to realize membrane positioning catalysis, phospholipid ketonization induction, membrane integrity destruction and tumor microenvironment remodeling. The host-guest intelligent material has excellent tumor targeting property, biocompatibility and anti-tumor effect, a ketone modified phospholipid structure capable of adjusting the tumor microenvironment is determined, and the application of the host-guest intelligent material in tumor intervention is expanded.

This invention discloses a method derived from Hoddle archaea ( Hodarchaeales This study describes the antimicrobial peptide SC1_966 and its applications, belonging to the field of biomedicine and aquaculture disease control technology. The amino acid sequence of the antimicrobial peptide is shown in SEQ ID NO:1. This antimicrobial peptide consists of 35 amino acids, with a molecular weight of 4330.30 Daltons, containing 10 basic amino acid residues. It has a hydrophobicity of -0.543 and an isoelectric point of 10.84, making it a cationic short peptide with advantages such as good water solubility and high antimicrobial activity. Its compact structure makes it easy to synthesize and purify, facilitating large-scale production and application. Furthermore, the presence of multiple positively charged amino acid residues facilitates interaction with the negatively charged bacterial cell membrane, disrupting membrane integrity and thus exerting rapid antibacterial and bactericidal effects. This antimicrobial peptide exhibits broad-spectrum and highly efficient antimicrobial activity against common aquatic pathogens such as *Escherichia coli* and various Vibrio species. At effective antimicrobial concentrations, it shows no significant cytotoxicity to HeLa bacteria, demonstrating good biocompatibility.

A controller (10), a solution generator (1) and a method for assessing the integrity of a forward osmosis (FO) membrane (2c) of a FO device (2) in a dialysate generator (1). The FO device (2) is configured for use in an FO session for diluting a dialysis concentrate in a process for generating dialysate. The FO membrane (2c) separates a first side (2a) of the FO device (2) from a second side (2b). The method includes passing (S1) an electrolyte solution through the first side (2a) and passing (S2) a low electrolyte solution through the second side (2b). The method further comprises measuring (S3) the conductivity of the solution produced from the second side (2b) and assessing (S6) the integrity of the FO membrane (2c) based on whether the measured conductivity satisfies a conductivity criterion, the conductivity criterion comprising or defining the conductivity of a solution produced from the second side (2b) using an intact or intact FO membrane with an equivalent electrolyte solution and an equivalent low electrolyte solution.

The invention belongs to the technical field of biological medicines, and particularly relates to an anti-aging mitochondrial preparation as well as a preparation method and application thereof. The mitochondrial preparation is formed by compounding mammalian-derived mitochondria and a pharmaceutically acceptable injection solvent, the membrane potential (delta psi m) of the mammalian-derived mitochondria is kept unchanged or reduced by 50%-90% compared with that before separation, and the outer membrane integrity rate is larger than or equal to 90%. Under the state that the membrane potential is reduced, the outer membrane structure is still kept complete, and the capability of inducing host cell PINK1 / Parkin dependent mitochondrial autophagy and network remodeling is achieved, so that inferior mitochondria is selectively removed, and mitochondrial neogenesis is promoted. The preparation can induce systemic mitochondrial quality remodeling in multiple tissues, significantly improve ATP level, reduce ROS generation, increase mitochondrial DNA copy number, down-regulate aging markers p16 and p21, and improve cognition, movement and organ functions in an old animal model.

The invention relates to the field of pig semen preservation, in particular to a 15-20 DEG C diluent of pig semen with ferulic acid as an additive as well as a preparation method and application of the 15-20 DEG C diluent. The proportion of the ferulic acid in the basic diluent is 0.2 to 0.8 mmol / L; wherein the basic diluent is prepared by dissolving 3.715 g of glucose, 0.125 g of sodium citrate, 0.125 g of sodium ethylene diamine tetracetate, 0.075 g of potassium chloride and 0.03 g of gentamicin sulfate into 100 mL of double distilled water, and after the materials are fully dissolved, the pH value of the solution is adjusted to 6.80-7.00, and the osmotic pressure of the solution is adjusted to 290-300 mOsm / kg. The method has the advantages that ferulic acid is used as a protective agent for preservation of the boar semen at 15-20 DEG C for the first time, the effect is reliable, popularization is easy, the preserved boar semen is suitable for artificial insemination and artificial insemination after long-distance transportation, the vitality, motility, acrosome integrity and plasma membrane integrity of boar sperms preserved at 17 DEG C can be remarkably improved, and the fertilization vitality of the sperms is kept.

The invention provides a method for extracting plant leaf outer vesicles based on heterophyte outer body fluid, and belongs to the technical field of preparation of plant outer vesicles. According to the method provided by the invention, the yield of the target outer vesicles can be remarkably improved, and the complete morphological structure and membrane integrity of the target outer vesicles can be better maintained. By optimizing an extraction and preservation system, aggregation and degradation of the vesicles in the preparation and storage process are effectively inhibited, and the long-term stability of the vesicles is guaranteed. The ectovesicles obtained by the method show remarkably enhanced physiological activity and treatment potential in disease model experiments, for example, the ectovesicles have excellent effects in the aspects of improving fatty liver pathology and promoting endothelial cell repair; a high-quality material basis and a reliable technical scheme are provided for developing innovative medicines or functional products based on the plant exovesicles.