18 results about "Acrosome" patented technology

The invention belongs to the technical field of biology, and particularly relates to application of N-Acetyl-D-Glucosamine as an additive in a boar semen diluent preserved at 17 DEG C. The invention further relates to a preparation method of the N-Acetyl-D-Glucosamine. By adding 2.5 mM of N-Acetyl-D-Glucosamine into the boar semen diluent, the activity and the forward movement proportion of boar sperms can be remarkably improved under the preservation condition of 17 DEG C, and meanwhile, no adverse effect is caused on the lipid oxidation state and acrosome integrity of the boar sperms. The method is easy and convenient to operate, low in cost, suitable for the artificial insemination process of a large-scale pig farm and capable of effectively prolonging the preservation time of the boar semen and improving the breeding efficiency.

This invention proposes a sperm staining kit based on a sequential staining strategy of tail pre-staining – rapid differentiation – enhanced nuclear staining. A gentle fixation with 4% paraformaldehyde and acetate buffer preserves the complete nucleic acid of the sperm head and the protein structure of the tail. The addition of PVP K30 and Tween-20 effectively inhibits non-specific adsorption and reduces staining background interference. Brilliant Green SF combined with sodium tartrate is used for specific staining of the sperm tail, followed by rapid differentiation to wash away non-specific staining. Nuclear staining is performed using toluidine blue O combined with sodium chloride and sodium tetraborate hydrate, specifically binding to DNA and enhancing color development stability, resulting in a blue sperm head and a light green tail with clear color contrast. This invention produces a clean staining background, accurately distinguishing the morphology of the sperm head, acrosome, and tail, significantly improving the accuracy of microscopic identification of abnormal sperm such as large-headed, small-headed, curled-tailed, broken-tailed, and short-tailed sperm. It is suitable for detecting sperm morphology and abnormality rates in breeding males.

The invention relates to the technical field of biological reproduction, and discloses a pig sperm ultra-long-acting dilution powder containing natural antibacterial components and a preparation method of the pig sperm ultra-long-acting dilution powder. The feed additive is prepared from the following raw materials in parts by weight: 30 to 50 parts of glucose, 1 to 2 parts of sodium ethylene diamine tetracetate, 5 to 10 parts of sodium citrate, 1 to 2 parts of sodium bicarbonate, 0.5 to 1 part of potassium chloride, 0.5 to 2 parts of composite natural antibacterial components, 0.2 to 0.8 part of novel antibacterial peptide, 0.3 to 0.8 part of polyvinyl alcohol, 0.05 to 0.2 part of cysteine and 1 to 3 parts of freeze-dried egg yolk powder. The pig sperm long-acting dilution powder prepared by the process has remarkable advantages, the sperm motility rate can be maintained to be 65% or above within 10 days at 17 DEG C, the total number of bacterial colonies is only 3.8 * 10 < 3 > CFU / mL or so within 10 days, the sperm plasma membrane integrity rate and the acrosome integrity rate reach 62% or above and 65% or above respectively after 10 days, the sperm long-acting dilution powder far exceeds traditional dilution powder, and the requirements of long-distance transportation and large-scale breeding are met.

The application provides a boar semen cryopreservation agent and an application method; the boar semen cryopreservation agent comprises alpha-amylase, and the content of the alpha-amylase is 10-1000 mu g / L. The application method of the cryopreservation agent is as follows: centrifuging the boar semen after collection and pretreatment, removing supernatant after centrifugation; adding a refrigerant, re-suspending the sperm, and then placing the sperm in a 4 DEG C refrigerator and standing for 3-4 hours; adding a freezing liquid and fully mixing; filling the semen into a thin tube at 4 DEG C, uniformly laying the thin tubes in a freezing rack, and then precooling on a liquid nitrogen surface and then placing in liquid nitrogen for freezing. The application has the advantages that: the overall components of the cryopreservation agent are simple, the cost is low, and the overall cost performance is high; due to the addition of alpha-amylase in the cryopreservation agent, the storage quality of the boar frozen semen can be significantly improved; and the activity, acrosome integrity, DNA integrity, mitochondrial activity and other physical and chemical properties of the boar frozen semen are effectively improved.

The invention discloses a method for improving the quality of male sperms. Belongs to the technical field of biological medicine. It is found that the sperm quality of male mice can be remarkably improved by long-term in-vivo application of D-mannose. The method is mainly embodied in that kinematics parameters of total sperm motility rate and forward motility rate are remarkably increased, and the effect is independent of gonad development improvement; if the sperm mitochondrial membrane potential rises, the energy metabolism function is improved; the sperm acrosome integrity is improved, and the sperm quality is integrally optimized. The results show that D-mannose, as a safe natural monosaccharide, can significantly improve the quality of male sperms in an in-vivo intervention manner, and provides a new method and approach for treatment and intervention of male infertility, especially asthenozoospermia.

The invention belongs to the technical field of animal breeding, and particularly relates to application of alpha-ketoglutaric acid or salt thereof in preparation of a sheep semen cryopreservation diluent. The invention discloses the application of alpha-ketoglutaric acid or salt thereof in the cryopreservation of the sheep semen for the first time, and the alpha-ketoglutaric acid or salt thereof can improve the semen quality of the frozen and unfrozen sheep semen and improve the fertilization ability of the frozen semen; such as sperm motility, sperm movement performance, sperm viability, sperm plasma membrane integrity rate, sperm acrosome integrity rate, sperm antioxidant capacity, sperm ATP level, sperm early apoptosis incidence rate, sperm in-vitro fertilized ovulation rate and / or artificial fertilization pregnancy rate, can be used for preparing the sheep sperm cryopreservation diluent.

This invention belongs to the field of animal husbandry technology and relates to a method for improving the quality of frozen semen from Lanzhou fat-tailed sheep based on ergothioneine. This application, through optimized combination of diluent, 8 layers of gauze wrapping for cooling, 4 cm fumigation height, and 8 mM ergothioneine, achieved a sperm motility rate of 45.80±3.62%, an acrosome integrity rate of 52.32±2.28%, and a plasma membrane integrity rate of 55.38±3.55% after thawing. All indicators are significantly better than those of the traditional single-optimization group.

This invention discloses a method for cryopreservation of donkey semen using antioxidants, belonging to the field of animal reproductive technology. The method is based on a specific basal diluent, with the addition of targeted mitochondrial antioxidants SKQ1 or SS-31. First, a basal diluent containing sugars, citrate, proline, skim milk powder, and antibiotics is prepared. Then, SKQ1 (dissolved in DMSO) or SS-31 (dissolved in physiological saline) is separately prepared into a stock solution, which is then mixed with the basal diluent to obtain a composite preservation solution. Finally, the donkey semen treated with this composite preservation solution is diluted and stored at 4°C. The method of this invention can significantly improve sperm motility and acrosome integrity rate of donkey semen stored at 4°C for 5 days, reduce ROS content and abnormality rate, and significantly increase the insemination and conception rate of the preserved semen to over 40%, effectively extending the practical preservation period of donkey semen.

The invention belongs to the technical field of biology. The invention relates to an antioxidant composition, in particular to an antioxidant composition and application thereof in normal-temperature preservation of boar semen. Researches find that the activity of boar sperms in the normal-temperature preservation period can be effectively improved by independently adding lycopene or antioxidant SKQ1, the combined use effect of lycopene and antioxidant SKQ1 shows a synergistic effect, and when the boar sperms are preserved to the seventh day, the average sperm activity value exceeds 70%. In addition, through combined treatment, the integrity of sperm acrosome and plasma membrane is remarkably maintained, the antioxidant enzyme activity of sperms is improved, the active oxygen and malonaldehyde level are effectively reduced, meanwhile, the mitochondrial membrane potential and the adenosine triphosphate content are remarkably improved, and the expression level of sperm P-AMPK and Nrf2 protein is improved. In conclusion, the lycopene and the SKQ1 are combined for use, oxidative stress and energy metabolism are synergistically regulated and controlled, the quality and function stability of the boar sperms in the normal-temperature preservation process are remarkably improved, and the lycopene and the SKQ1 have good application value and application prospects.

The invention discloses a sperm acrosome reaction detection method based on phase imaging, which comprises the following steps: preparing two sperm smears, obtaining a phase diagram SP of sperms without acrosome reaction, and obtaining a phase diagram SPacrosome of sperms with acrosome reaction; obtaining a sperm nucleus average phase value SPt, a sperm acrosome average phase value SPd, a sperm nucleus average phase value SPtarosome and a sperm acrosome average phase value SPdacrosome; calculating a first difference value between the SPt and the SPd and a second difference value between the SPtasrosome and the SPdarosome, and calculating a second difference value between the SPtasrosome and the SPdarosome; calculating the phase ratio R of the sperm acrosome to the sperm nucleus and the phase ratio Racrosome of the sperm acrosome to the sperm nucleus; and through comparative analysis of the SPt, the SPd, the R, the SPtarosome, the SPdacrosome and the Racrosome, a distinguishing and judging rule of the sperms without the acrosome reaction and the sperms with the acrosome reaction is obtained. The sperm acrosome reaction detection method based on phase imaging has the advantages that vacuole detection can be carried out without fluorescence staining on sperms, unmarked quantitative measurement on the sperms is realized, the detection efficiency and accuracy are high, and the like.

The invention relates to an additive of a diluent for preserving boar semen at 17 DEG C and an application method thereof. Myoinositol with the concentration of 1 mg / mL is added into a boar semen diluent, so that the activity and forward movement proportion of boar sperms under the preservation condition of 17 DEG C can be remarkably improved, and meanwhile, the lipid oxidation state and acrosome integrity of the boar sperms are not influenced. The method is easy and convenient to operate, low in cost, suitable for the large-scale pig farm artificial insemination technology and beneficial to prolonging the pig semen preservation time and improving the breeding efficiency.

The invention relates to the field of boar semen preservation, in particular to preparation and application of a ferulic acid-based boar semen low-temperature diluent. The proportion of the ferulic acid in the basic diluent is 0.2 to 0.8 mmol / L; wherein the basic diluent is prepared by dissolving 3.00 g of glucose, 4.00 g of cane sugar, 0.03 g of gentamicin sulfate and 20 mL of yolk in 80 mL of double distilled water, uniformly stirring, centrifuging at 4 DEG C and 7000 * g for 25 min, adjusting the pH value of the solution to 6.80-7.00, and adjusting the osmotic pressure of the solution to 290-300 mOsm / kg. The method has the beneficial effects that ferulic acid is used as a protective agent for preserving the boar semen at 4 DEG C for the first time, the effect is reliable, popularization is easy, the preserved boar semen is suitable for artificial insemination and artificial insemination after long-distance transportation, the motility, motility rate, acrosome integrity rate and plasma membrane integrity rate of boar sperms preserved at 4 DEG C can be remarkably improved, and the fertilization activity of the sperms is kept.

The invention belongs to the technical field of animal breeding, and particularly relates to application of alpha-ketoglutaric acid or salt thereof in preparation of a normal-temperature sheep semen preservation diluent. The invention discloses the application of alpha-ketoglutaric acid or salt thereof in normal-temperature preservation and / or dilution of the sheep semen for the first time, and the semen quality of the sheep semen during normal-temperature preservation and / or dilution can be improved by the alpha-ketoglutaric acid or salt thereof; such as sperm motility rate, sperm motility, sperm motility performance, sperm viability, sperm plasma membrane intact rate, sperm acrosome intact rate and / or artificial fertilization pregnancy rate, can be used for preparing the sheep sperm normal-temperature preservation diluent.

The invention discloses a diluent for normal-temperature preservation of boar semen and a preservation method. Selenomethionine is selected as a protective agent in the diluent for preserving the boar semen at the normal temperature to improve the semen quality, the diluent for preserving the semen at the low temperature is a Modena basic diluent, and the addition amount of the selenomethionine in the diluent for preserving the semen at the normal temperature is 0.1-10 mg / L, preferably 1 mg / L. According to the invention, selenomethionine is added into the diluent for preserving the boar semen at normal temperature, so that the motility, motility rate, plasma membrane integrity rate and acrosome integrity rate of the boar semen can be remarkably improved. If the method is applied to actual production, the normal-temperature preservation time of the boar semen can be prolonged, the semen preservation quality is improved, and the production cost is reduced.

The application discloses a sperm quality detection device and a preparation method thereof. The sperm quality detection device comprises a base material, a sperm activity detection module, an SP10 detection module and a PSA detection module. The sperm activity detection module comprises a containing cavity and a convex lens. The containing cavity has a detection plane. The convex lens is configured to be able to slide to a sample adding hole along with a sliding assembly and to magnify the image of the detection plane. The SP10 detection module comprises a P10 colloidal gold test strip. The PSA detection module comprises a PSA colloidal gold test strip. The application provides a detection device integrating sperm acrosome enzyme SP10 protein, prostate specific antigen PSA and special counting for a semen sample. The device is simple to operate and can provide a detection result in a short time. The detection result can be judged by the person to be detected independently, and the device is suitable for primary medical units and on-site rapid detection.

The invention relates to the field of pig semen preservation, in particular to a 15-20 DEG C diluent of pig semen with ferulic acid as an additive as well as a preparation method and application of the 15-20 DEG C diluent. The proportion of the ferulic acid in the basic diluent is 0.2 to 0.8 mmol / L; wherein the basic diluent is prepared by dissolving 3.715 g of glucose, 0.125 g of sodium citrate, 0.125 g of sodium ethylene diamine tetracetate, 0.075 g of potassium chloride and 0.03 g of gentamicin sulfate into 100 mL of double distilled water, and after the materials are fully dissolved, the pH value of the solution is adjusted to 6.80-7.00, and the osmotic pressure of the solution is adjusted to 290-300 mOsm / kg. The method has the advantages that ferulic acid is used as a protective agent for preservation of the boar semen at 15-20 DEG C for the first time, the effect is reliable, popularization is easy, the preserved boar semen is suitable for artificial insemination and artificial insemination after long-distance transportation, the vitality, motility, acrosome integrity and plasma membrane integrity of boar sperms preserved at 17 DEG C can be remarkably improved, and the fertilization vitality of the sperms is kept.

The Mogroside extract has the effect of improving the damage of the heat stress boar's reproductive capacity. Research shows that, in the process of heat stress of boar, by adding Mogroside extract in the basic diet, the damage of reproductive capacity caused by heat stress can be effectively improved. The test data shows that the Mogroside extract can significantly improve the sperm density, effective sperm number, sperm motility and reduce the sperm abnormality rate of boar; significantly increase the content of a-glucosidase, lactate dehydrogenase, acrosome enzyme and hyaluronidase in the semen, and increase the concentration of testosterone in the serum, enhance the energy supply, structural integrity, functional maturity and fertilization capacity of sperm. Therefore, the Mogroside extract has the potential for further development in the treatment and prevention of heat stress boar's reproductive capacity damage, and can be used for preparing the medicine for improving the heat stress boar's reproductive capacity, so as to further develop the health care function and medicinal value of Siraitia grosvenorii.

The application relates to a method, device, computer equipment and storage medium for diagnosing the result of routine in vitro fertilization of sperm. The method for diagnosing and predicting the result of routine in vitro fertilization comprises the following steps: calculating the PLCzeta positive rate of a sperm sample, the PLCzeta positive rate being the proportion of the number of PLCzeta positive sperm in the sperm sample in the total number of sperm, the PLCzeta positive sperm being sperm in which PLCzeta can be detected in at least one of the following head regions of the sperm: acrosome, equator and post-acrosomal region; and diagnosing the fertilization result of routine in vitro fertilization according to the PLCzeta positive rate. The method can efficiently judge the fertilization capacity of sperm that has undergone or is to undergo routine in vitro fertilization, find sperm that may have low fertilization or fertilization failure in routine in vitro fertilization due to PLCzeta defects, provide a basis for the development of a fertilization scheme, and prevent the occurrence of fertilization failure or low fertilization.