51 results about "Total antioxidant" patented technology

The invention provides a pine pollen fermentation product with anti-wrinkle and anti-aging functions as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. The pine pollen extract is prepared by fermenting lactobacillus plantarum, the total polysaccharide content of the obtained pine pollen fermentation product is maximally increased by 77.3%, the FRAP total antioxidant capacity is maximally increased by 2.85 times compared with that of the unfermented pine pollen fermentation product, the anti-wrinkle effect of human skin cells can be effectively improved, and the pine pollen extract has a relatively strong proliferation effect on an ultraviolet damage model of human skin fibroblasts; the pine pollen extract has the advantages that the pine pollen extract is fermented, the elastin content is remarkably increased, the life of nematodes is effectively prolonged, lipofuscin deposition is reduced, and compared with unfermented pine pollen extract, the anti-aging capacity of the pine pollen can be effectively improved; the preparation method provided by the invention is low in price and simple in process, and the active ingredients and the efficacy of the traditional Chinese medicine composition are improved in a simplest and effective manner.

The invention discloses MnO2 (at) ZIF-67 composite nanoparticles with high oxidase activity as well as a preparation method and application of the MnO2 (at) ZIF-67 composite nanoparticles. The preparation method comprises the following steps: uniformly mixing a potassium permanganate aqueous solution and a cerous nitrate aqueous solution to obtain a mixed solution I; and adding nitric acid into the mixed solution I, carrying out hydrothermal reaction to obtain a substance MnO2, uniformly mixing the obtained MnO2 and a methanol solution of PVP, adding cobalt nitrate hexahydrate and 2-methylimidazole, and standing to obtain the MnO2 ZIF-67 composite nanoparticles. The nano-particles with high enzymatic activity are prepared through a simple hydrothermal synthesis method, have good oxidase mimetic activity, catalyze O2 and TMB to rapidly develop color and become dark blue, and can specifically, sensitively and rapidly detect the total antioxidant capacity of cells, so that the nano-particles have good practical application value.

The invention belongs to the technical field of plant breeding in molecular biology, and provides a cotton GhVIP1 gene and application thereof in drought resistance and salt tolerance of plants. The nucleotide sequence of the GhVIP1 gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the GhVIP1 gene is as shown in SEQ ID NO: 2. The gene is obviously up-regulated under the induction of salt and drought stress. An arabidopsis thaliana homozygous line overexpressed with the GhVIP1 gene is obtained through a transgenic technology, under the stress of drought and salt, the seed germination rate of the arabidopsis thaliana overexpressed with the gene is higher than that of a wild type, the growth condition of a transgenic line in the seedling stage is better than that of the wild type, the stem length, the total antioxidant capacity and the chlorophyll content are all superior to those of the wild type, and the MDA content is lower than that of the wild type; further, the drought resistance and the salt resistance of the arabidopsis thaliana are improved. When the gene is applied to drought-resistant and salt-resistant breeding of plants, the drought resistance and salt resistance of the plants can be improved, and an effective alternative scheme is provided for molecular breeding of drought-resistant and salt-resistant crops.

The invention discloses a preparation method of ganoderma lucidum mutant strain active polysaccharide and application of the ganoderma lucidum mutant strain active polysaccharide in oxidation resistance, and relates to the technical field of biology. The ganoderma lucidum mutant strain active polysaccharide is prepared by taking mycelium of a ganoderma lucidum mutant strain CGMCC No.23283 as a raw material and adopting a water extraction and alcohol precipitation method. The invention develops a novel ganoderma lucidum active polysaccharide, and a ganoderma lucidum strain adopted by the ganoderma lucidum active polysaccharide is cultured by a space mutation technology. Compared with common ganoderma lucidum strain polysaccharide, the polysaccharide provided by the invention is more prominent in antioxidant activity, and is more excellent in key indexes such as total antioxidant capacity and free radical scavenging. The invention provides a new biological material resource for the preparation of antioxidant products.

The application discloses a bacillus licheniformis YL-001 with anti-inflammatory and antibacterial effects and an application thereof, and belongs to the technical field of biotechnology. The bacillus licheniformis YL-001 is separated from soil. The preservation number of the bacillus licheniformis is GDMCC No.66556. The metabolic product of the bacillus licheniformis YL-001 provided in the application has the abilities of significantly inhibiting secretion of inflammatory factors and growth of streptococcus pneumoniae and hemolytic streptococcus b, and can improve the total antioxidant capacity of serum. The strain has good biocompatibility and high safety, has important significance for development of anti-inflammatory or antibacterial drugs, health products and food, and is especially suitable for preparation of products with throat-protecting effects.

The invention belongs to the technical field of biology, and particularly relates to acetylated raffinose and application thereof. According to the invention, acetylation modification is carried out on raffinose by using an acetylation reagent to obtain acetylated raffinose, the acetylated raffinose can significantly improve the scavenging ability and total antioxidant ability of raffinose on DPPH free radicals, and the scavenging ability on hydroxyl free radicals and the reduction ability on iron ions are also improved to a certain extent. The acetylated raffinose provided by the invention has potential application value in development of functional foods and antioxidants.

The invention relates to a preparation method of ultrathin two-dimensional cobalt oxide rich in oxygen vacancies and an application of the ultrathin two-dimensional cobalt oxide rich in oxygen vacancies in food total antioxidant capacity detection, and belongs to the fields of low-dimensional nano materials, biological mimic enzyme catalysis and analytical chemistry. According to the preparation method, cobalt acetylacetonate and hexadecyl trimethyl ammonium bromide are adopted as raw materials, and the ultrathin two-dimensional cobalt oxide nano-enzyme rich in oxygen vacancies is prepared through a hydrothermal reaction and high-temperature pyrolysis. The obtained ultrathin two-dimensional cobalt oxide nano-enzyme rich in oxygen vacancies has excellent peroxidase-like catalytic activity and selectivity. The oxygen-vacancy-rich metal oxide has the advantages of high stability, easy mass production and low preparation cost, and is expected to be widely used in the fields of biosensing, agriculture, food engineering, catalysis and the like.

The application is suitable for the field of analog enzyme technology, and provides a light-controlled zein-based dual-enzyme-activity analog enzyme and a preparation method thereof.The preparation method comprises the following steps: under water bath conditions, dissolving zein in an alkaline aqueous solution and incubating; adding a hydroxylamine solution to the solution obtained in step S1 and continuing to react; and after the reaction is completed, obtaining the light-controlled zein-based dual-enzyme-activity analog enzyme.The application uses zein as a raw material, is modified by hydroxylamine, does not need any metal component to participate, has light-controlled switchable dual-enzyme activity, exhibits oxidase-like activity under visible light irradiation, presents peroxidase-like activity in dark conditions, and can realize reversible switching of the two enzyme activities by regulating light, effectively overcomes the problems that traditional artificial analog enzymes depend on metal ions, multiple enzyme activities coexist, interfere with each other and are difficult to accurately control, can be used for colorimetric detection of total antioxidant capacity, can be used for colorimetric detection of glucose in cascade reaction with glucose oxidase, has the advantages of simple operation, rapid response, high sensitivity, good selectivity, low cost and the like, and has a good application prospect in the fields of food analysis, clinical diagnosis and the like.

The invention provides a method for detecting the total antioxidant capacity of saliva based on iron-based MOF nano-enzyme, and belongs to the field of biosensing. The method comprises the following steps: obtaining an MIL-53Fe nano enzyme; the preparation method comprises the following steps: mixing a diluent of MIL-53Fe nano-enzyme, a sodium acetate buffer solution, 3, 3 ', 5, 5'-tetramethyl benzidine and hydrogen peroxide for reaction, adsorbing a reaction solution by using filter paper, drying and curing by using a chitosan solution to obtain the visual microfluidic detection paper-based chip. Establishing a standard curve of the chromatic value and the ascorbic acid concentration; dropwise adding the treated saliva sample onto the paper-based chip, standing for reaction to obtain a chromatic value, and calculating according to the standard curve to obtain the total antioxidant capacity value of the saliva. Complex matrix interference is broken through, the detection sensitivity and portability are improved, and therefore clinical application of saliva TAC detection in noninvasive health monitoring is promoted.

The invention discloses bacillus licheniformis YL-001 with anti-inflammatory and antibacterial effects and application of the bacillus licheniformis YL-001, and belongs to the technical field of biology, and the bacillus licheniformis YL-001 is separated from soil. The preservation number of the bacillus licheniformis strain is GDMCC (China General Microbiological Culture Collection Center) No.66556. A metabolite of the bacillus licheniformis YL-001 provided by the invention has obvious capabilities of inhibiting secretion of inflammatory factors and inhibiting growth of streptococcus pneumoniae and beta hemolytic streptococcus, and can improve the total antioxidant capacity of serum, and the strain is good in biocompatibility, high in safety and suitable for industrial production. The method has important significance for developing anti-inflammatory or bacteriostatic medicines, health care products and foods, and is particularly suitable for preparing products with the sore-throat relieving effect.

The application relates to the field of analytical detection technology, and discloses a bimetallic MOF nano-enzyme as well as a preparation method and application thereof. The bimetallic MOF nano-enzyme comprises iron, cerium and an organic skeleton, the organic skeleton is 1,3-benzenedicarboxylic acid, and the iron and the cerium replace H atoms in carboxyl groups of the organic skeleton. The preparation method comprises the following steps: dissolving FeCl3.6H2O and Ce(SO4)2.4H2O in a solvent to obtain solution A; dissolving 1,3-benzenedicarboxylic acid in a DMF solution to obtain solution B; and mixing and reacting the solution A with the solution B to obtain the bimetallic MOF nano-enzyme. The bimetallic MOF nano-enzyme is used for quantitative detection of total antioxidant capacity, glutathione, ascorbic acid and cysteine, has the advantages of high stability, high reliability, high sensitivity, good selectivity, simple operation and the like, and can be widely applied in the fields of biomedicine, food safety, environmental monitoring and the like.

The application discloses a preparation method of high-quality bran raw materials, comprising the following steps: 1) washing, drying, powdering, sterilizing and reserving wheat bran; 2) adding edible fungus seed liquid into the wheat bran and uniformly mixing and fermenting to prepare high-quality bran raw materials. The application also discloses the high-quality bran raw materials obtained by the preparation method and application thereof. After fermentation, the water absorption index, water-solubility index and swelling degree of the bran are significantly increased, wherein the water-solubility index is increased by 277% at most, the total phenol content is 5.91 times of that of the unfermented sample at most, the alkyl resorcinol content is 1.55 times of that of the unfermented sample at most, and the total antioxidant capacity of the raw material is improved to 311.23 U / g, which is 5.73 times of that of the unfermented sample. Meanwhile, the fermented raw material generates alcohol aroma substance 4-ethyl guaiacol, which further improves the quality of the raw material.

The application discloses application of metformin in prolonging egg laying time of pigeons, and the metformin prolongs the egg laying time of the pigeons through a glycolysis pathway, and the mass ratio of a dosage of the metformin to a body weight of the pigeons is 60-65 mg:1 kg. It is found through experiments that the metformin can improve oxidative stress, effectively improve total antioxidant capacity in pigeon serum, reduce contents of H2O2 and MDA in the serum, rapidly improve production performance of the pigeons, has effects of promoting nutrient absorption of the body, rapidly recovering damaged functions of the body, and synergistically exerting antioxidation, and simultaneously has advantages of easy absorption of the body, improvement of bioavailability of the medicine and the like.

The invention discloses a cerium-based polyoxometallate / metal organic framework-based composite nano-enzyme as well as a preparation method and detection application of the cerium-based polyoxometallate / metal organic framework-based composite nano-enzyme. The composite material is prepared by reacting ferric chloride, cerium-based polyacid and 2-aminoterephthalic acid, and has excellent peroxidase-like activity, stability and substrate affinity. Based on the material, the invention further develops a recyclable paper-based detection device, and the paper-based detection device is matched with a smart phone color recognition system to construct a rapid colorimetric detection platform for the antioxidant. The platform can realize high-sensitivity and high-selectivity detection of various antioxidants such as ascorbic acid, glutathione, gallic acid and the like, and is successfully applied to total antioxidant capacity analysis of actual samples such as fruits and the like. Compared with a traditional method, the method has the advantages of being rapid in detection, easy and convenient to operate, low in cost, environmentally friendly and the like, and is suitable for the field of food safety on-site rapid monitoring.

The invention belongs to the technical field of fruit wine processing, and particularly relates to wolfberry and green plum compound fruit wine and a preparation method thereof. The invention provides a preparation method of Chinese wolfberry fruit and green plum compound fruit wine, which comprises the following steps: performing primary fermentation on green plums subjected to pretreatment and sugaring treatment to obtain green plum raw wine, and performing secondary fermentation on green plum residues generated in the primary fermentation and Chinese wolfberry fruits to obtain green plum and Chinese wolfberry fruit compound raw wine, and finally, uniformly mixing the green plum raw wine and the green plum and Chinese wolfberry fruit composite raw wine, and performing post-treatment, ageing and refining to obtain the Chinese wolfberry fruit and green plum composite fruit wine. A two-round fermentation process of'sugaring main fermentation-low-temperature synergistic fermentation 'is adopted, so that the content of anthocyanin, the content of flavone, the content of total phenols and the total oxidation resistance in the composite fruit wine are greatly improved; finally, the compound fruit wine which is elegant in color and luster, coordinated in flavor and remarkably higher in anthocyanin content, antioxidant activity and the like than single-raw-material fruit wine is obtained.

The invention relates to the technical field of animal growth regulation, in particular to application of all-trans retinoic acid in regulation of oxidation resistance and digestion and metabolism capacity of Ningxiang pigs. According to the application, all-trans-retinoic acid is added into the Ningxiang pig feed, and the research on the antioxidant capacity of intestinal microorganisms and serum of the Ningxiang pig feed shows that all-trans-retinoic acid can improve the antioxidant capacity of the Ningxiang pig by enhancing the total antioxidant capacity of the Ningxiang pig, changing the diversity of intestinal flora of the Ningxiang pig and changing microbial metabolites and metabolic pathways of the intestinal flora; the biosynthesis and metabolism of amino acids are promoted, so that the oxidation resistance and digestion and metabolism capabilities of the Ningxiang pigs are enhanced.

The invention discloses a capsule medicine for treating hyperlipemia as well as a preparation method and application thereof, and belongs to the technical field of hot-dip galvanized steel plates, the capsule medicine is prepared from the following raw materials in percentage by weight: 58-69% of egg extract, 8.3-10.3% of tea polyphenol, 13.2-18.6% of sophora flavescens extract and 7.1-13.1% of turmeric extract. Through the mode, the egg extract improves antioxidant indexes of liver VLDL, superoxide dismutase, glutathione peroxidase, total antioxidant capacity and the like and reduces accumulation of plasma triglyceride, total cholesterol and the like, the tea polyphenol and the sophora flavescens extract are beneficial for improving the activity of the egg extract, the effect of the egg extract is further improved, and meanwhile, the effect of the egg extract is further improved. In addition, the tea polyphenol can inhibit the generation of fat and effectively reduce energy accumulation, and in addition, the turmeric extract is beneficial to accelerating the rapid discharge of triglyceride and total cholesterol after removal, so that the treatment of hyperlipidemia is realized.

The invention discloses a lactobacillus rhamnosus CQFP202457 and application of the lactobacillus rhamnosus CQFP202457 and ellagic acid in relieving exercise fatigue, and belongs to the technical field of microorganisms. The preservation number of the lactobacillus rhamnosus CQFP202457 is CGMCC (China General Microbiological Culture Collection Center) No. 29784. The composition provided by the invention contains ellagic acid and lactobacillus rhamnosus CQFP202457, and can significantly prolong the exercise exhaustion time, reduce the content of fatigue-related metabolites in serum, improve the total antioxidant ability of serum, liver and skeletal muscle and the antioxidant enzyme gene expression level, and improve the exercise-induced liver and skeletal muscle tissue damage. The strain and the composition thereof provided by the invention can be used as exercise synbiotics for preparing products for relieving exercise-induced fatigue, improving exercise performance, reducing exercise-induced oxidative stress or relieving exercise-induced muscle injury.

This invention discloses Moringa leaf polysaccharides with both immune-enhancing and antioxidant activities, as well as their preparation and applications. The method involves liquid nitrogen pulverization, ethanol purification, drying, cellulase hydrolysis, high-pressure homogenization, high-temperature water extraction, centrifugation, alcohol precipitation, ultrafiltration, and freeze-drying to prepare Moringa leaf polysaccharides. The obtained Moringa leaf polysaccharides exhibit good antioxidant activity, showing excellent performance in DPPH, ABTS free radical scavenging, and FRAP total antioxidant capacity assays. Simultaneously, they can promote NO secretion from RAW264.7 macrophages and enhance their phagocytic activity, demonstrating good immune-enhancing activity. The process of this invention is relatively simple, has good applicability in the food industry, and can be applied to various types of food processing.

The invention belongs to the technical field of microorganisms, and particularly provides a plant lactobacillus BUCM-1, the plant lactobacillus BUCM-1 is preserved in the China General Microbiological Culture Collection Center on June 16, 2025, the classification name of the plant lactobacillus BUCM-1 is plant lactobacillus, and the preservation number of the plant lactobacillus BUCM-1 is CGMCC NO. 34895. The plant lactobacillus BUCM-1 is named as plant lactobacillus. The lactobacillus plantarum BUCM-1 has relatively strong inhibitory ability to pancreatic lipase, relatively high total antioxidant ability and excellent cholesterol and triglyceride reducing ability, the survival rate is not greatly influenced after the lactobacillus plantarum BUCM-1 is cultured in artificial gastric juice for 3 hours, and the survival rate can be 65% after the lactobacillus plantarum BUCM-1 is continuously cultured in artificial intestinal juice for 8 hours. The invention further provides a preparation method of the plant lactobacillus fermented soybean juice, the process is simple, the quality is stable, and the prepared soybean juice has the effect of reducing blood fat.

The invention provides a gold nanorod multi-mode detection method and system based on a catalysis-etching cascade reaction and application of the gold nanorod multi-mode detection method and system. The method comprises the following steps: providing AuNR1 with oxidase-like activity and AuNR2 with near-infrared LSPR (Local Surface Plasmon Resonance) peak and photothermal effect; the method comprises the following steps: catalyzing TMB by using AuNR1 to generate oxTMB; introducing a to-be-detected sample to enable a target object to act with oxTMB; the method comprises the following steps: introducing AuNR2 under an acidic condition, and etching the AuNR2 by using an oxTMB derivative; the total antioxidant capacity and nitrite are synchronously quantified by detecting absorbance change, LSPR peak displacement or temperature rise change of AuNR2 in a near-infrared region. The invention also provides a detection system for realizing the method, so as to solve the problems that the existing scheme depends on an exogenous reagent, is poor in stability and inconvenient to quickly apply on site and the like. The method has the advantages of high anti-interference capability and high detection speed, and can meet the quality safety detection requirements of formula milk powder for special medical purposes.

The invention belongs to the technical field of xanthoxylin application, and particularly discloses application of xanthoxylin WGX-50 in preparation of a medicine for treating pathogenic bacteria induced broiler inflammatory depletion diseases, and the xanthoxylin WGX-50 can be used as a poultry feed additive. In-vitro experiments show that 20 [mu] M of WGX-50 significantly alleviates the toxicity of LPS-induced chicken macrophages, and the cell activity is improved to 103.74%. In-vivo 21d broiler chicken feeding tests prove that by adding 100mg / kg WGX-50 into daily ration, a synergistic effect can be generated: the total antioxidant capacity of an organism is remarkably enhanced, and the level of pro-inflammatory cytokines is reduced; meanwhile, the average daily gain of the broiler chickens is remarkably increased, the feed-gain ratio is reduced, and the metabolic rate of key nutrient substances such as crude protein, calcium and phosphorus is increased. According to the invention, a brand new solution is provided for the WGX-50 in the aspects of precise prevention and control of poultry inflammatory depletion diseases and improvement of breeding benefits through a clear action mechanism and dosage.

The present application belongs to the technical field of bioengineering, and particularly relates to a tuna fish bone antioxidant oligopeptide and a preparation method thereof.The tuna fish bone antioxidant oligopeptide has good scavenging activity, and has good thermal stability and digestion resistance; the mechanism of action is to promote the release of nitric oxide (NO), improve the levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), catalase (CAT) and total antioxidant capacity (T-AOC), and reduce the levels of malondialdehyde (MDA) and lactate dehydrogenase (LDH); JQYG-1 can protect HUVEC cells from oxidative damage induced by hydrogen peroxide; JQYG-1 can exert an antioxidant effect and protect vascular endothelial cells, and can provide a candidate drug for the prevention and treatment of cardiovascular and cerebrovascular diseases such as hypertension, coronary heart disease, cerebral thrombosis, myocardial infarction, atherosclerosis and heart failure.

The invention relates to an SNP (Single Nucleotide Polymorphism) molecular marker related to total antioxidant capacity of fresh semen sperms of local breeding rabbits in Fujian and application of the SNP molecular marker. The nucleotide sequence of the SNP molecular marker is shown as SEQ ID No: 1; in the nucleotide sequence of the SNP molecular marker, the 72nd basic group from the 5'end is represented by M, M represents C or T, and the locus is related to the total antioxidant capacity of fresh semen sperms of local breeding rabbits in Fujian; the total antioxidant capacity of fresh semen and sperms of the local breeding Fujian rabbits with the genotype of homozygous TT at the site is remarkably lower than that of the local breeding Fujian rabbits with the genotype of homozygous CC and heterozygous CT at the site. The SNP molecular marker disclosed by the invention is closely related to the total antioxidant capacity of fresh semen sperms of the local breeding rabbits in Fujian, and can be effectively used for molecular marker-assisted breeding of the local breeding rabbits in Fujian. The detection method established by the invention can be used for rapidly, efficiently and accurately detecting the total antioxidant capacity of the fresh semen sperms of the Fujian local breeding rabbits.

The invention provides an eel compound feed rich in hermetia illucens powder as well as a preparation method and application of the eel compound feed, and particularly belongs to the technical field of feeds. The compound feed for the eels comprises the following raw materials: hermetia illucens powder, fish meal, alpha-starch, fermented soybean meal, vital gluten, monocalcium phosphate, multiple vitamins, choline, multiple minerals, an adhesive, beer yeast, sodium chloride and aronia melanocarpa. According to the feed, imported fish meal in a conventional eel feed formula is partially replaced with the hermetia illucens powder, a proper amount of aronia melanocarpa is added, and the feed has good food calling performance and palatability and is suitable for breeding of eels in all growth stages from young eels to adult eels; the content of phosphorus in the feed and the cost of the feed are greatly reduced while the growth performance of the eels, the utilization efficiency of the feed and the survival rate of the eels are guaranteed; in addition, the feed not only can improve the intestinal health of the eels and improve the total antioxidant capacity of fish bodies, but also has a liver protection effect, and improves the economic benefits of eel culture.

This application belongs to the field of biotechnology, specifically to a preparation method and application of rose extract, which uses microbial fermentation technology to increase the content of water-soluble vitamins in rose. The strain selected in this method is Lactobacillus plantarum ( L. plantarum ), Saccharomyces cerevisiae ( S. cerevisiae ) and Bacillus subtilis ( B. subtle ), adjusting the strain ratio to achieve a viable bacterial count ratio of Lactobacillus plantarum, Saccharomyces cerevisiae, and Bacillus subtilis of (1-3):(1-2):(1-2), forming a composite inoculum, which was then added to pretreated roses for staged fermentation. This ultimately increased the water-soluble vitamin content in the rose extract, while retaining rose polysaccharides and increasing the total antioxidant activity by approximately 35%. This rose extract is suitable for use as a raw material for functional foods, health products, and cosmetics.

The invention belongs to the technical field of food processing, and relates to a phenolic acid-hydrolyzed casein compound as well as a preparation method and application thereof. Specifically, casein is hydrolyzed by alkaline protease to obtain casein with the molecular weight of 1t; a high antioxidant active component of 3KDa; the casein hydrolysate is mixed with a phenolic acid solution to form a compound. An in-vitro simulated gastrointestinal digestion test shows that the polyphenol content and the total antioxidant capacity of the compound are obviously higher than those of a single phenolic acid group, and the digestion stability of phenolic acid is improved. Animal experiments prove that the compound intervention can shorten the defecation time and improve the gastrointestinal propulsion rate, and the digestion promoting effect is superior to that of a single component and is equivalent to that of a positive drug mosapride; wherein the caffeic acid and the p-coumaric acid-casein hydrolysate compound have the best effect, and the synergy index is CIlt; 1, a synergistic effect is proved;

The invention discloses a pharmaceutical composition and application thereof. The pharmaceutical composition provided by the invention comprises the following active components in percentage by weight: 10-50% of geniposide, 10-50% of a surfactant, and 10-50% of a 10% to 50% of geniposide; 10% to 50% of genipin gentiobioside; 10 to 50 percent of crocin I and 10 to 50 percent of ursolic acid. The composition can effectively inhibit paralysis of tremor, improve the balancing capacity and grasping capacity of mice, reduce the content of interleukin-1beta and tumor necrosis factor-alpha, promote secretion of dopamine, reduce the content of malondialdehyde and improve the total antioxidant capacity in brain tissue. The compound has a good treatment effect on a Parkinson's disease model, so that the effect of treating the Parkinson's disease is expected to be greatly improved, and adverse reactions are reduced.

This invention discloses a plant rhizosphere growth-promoting bacterium, *Aristolochia nitroguaiacos* (…). Paenarthrobacter nitroguajacolicus YD-2-4, deposited at the China Microbial Culture Collection Center (CGMCC) under accession number CGMCC No. 36649, possesses various growth-promoting properties, including nitrogen fixation, phosphorus solubilization, IAA production, and ACC deaminase production. It significantly promotes the growth of forage grasses such as alfalfa, wheatgrass, crested wheatgrass, red fescue, Artemisia arenaria, and Stipa purpurea. The strain promoted shoot growth by 25.98% and 14.85% in Artemisia arenaria and red fescue, respectively, and root growth by 27.98% and 19.85% in Artemisia arenaria and Stipa purpurea, respectively. It also increased biomass accumulation in Artemisia arenaria and red fescue by 36.60% and 22.91%, respectively. Regarding plant antioxidant capacity, YD-2-4 significantly enhanced peroxidase activity in Artemisia arenaria and alfalfa, and increased the total antioxidant capacity of red fescue and Stipa purpurea. Strain YD-2-4 can serve as an excellent plant rhizosphere growth promoter, and has broad application prospects in the microbial remediation of degraded grasslands in alpine regions and the development of biofertilizers.

The invention discloses a transgenic breeding method for simultaneously synthesizing beta-carotene and alpha-linolenic acid in crop seed endosperm. The method comprises the following steps: carrying out sequence optimization on four genes PSY, CrtI, ORHis and FAD3 according to a breeding crop, fusing the four genes after sequence optimization with an endosperm expression promoter of the breeding crop respectively to construct gene expression cassettes respectively, assembling the four gene expression cassettes into a plant transformation vector containing PSY, CrtI, ORHis and FAD3, and finally transforming the plant transformation vector into the breeding crop to obtain the plant transformation vector containing PSY, CrtI, ORHis and FAD3. The seed endosperm of the transgenic crop is deep yellow, and the transgenic crop is synthesized with beta-carotene and alpha-linolenic acid. The beta-carotene and the alpha-linolenic acid are synthesized in the endosperm of the crop seeds, the storage stability of the beta-carotene in the seeds is improved, and the total antioxidant capacity of the seeds is improved. The product can be directly eaten, and has important guiding significance and production application value for breeding of novel composite functional crops.