78 results about "Total antioxidant" patented technology

The invention provides lactobacillus plantarum XZFMCC101.23155, a probiotic agent and application of the lactobacillus plantarum XZFMCC101.23155 and belongs to the technical field of microorganisms. The invention provides lactobacillus plantarum XZFMCC101.23155, and the preservation number of the lactobacillus plantarum XZFMCC101.23155 is GDMCC No: 65783. The lactobacillus plantarum XZFMCC101.23155 can tolerate hydrogen peroxide, a fermentation product of the lactobacillus plantarum XZFMCC101.23155 has the capacity of removing DPPH, hydroxyl free radicals and superoxide anions, and the total antioxidant activity is high. The fermentation product can also significantly reduce the MDA content in mouse serum, significantly improve the SOD level in mouse serum, improve the GSH content in mouse serum and the like. The lactobacillus plantarum XZFMCC101.23155 and commercial zymophyte are jointly used as a leavening agent to prepare the cheese, and the antioxidant activity of the cheese can be remarkably improved.

The invention belongs to the technical field of biology, and particularly relates to a preparation method and application of a rose extract, and the content of water-soluble vitamins in roses is increased by utilizing a microbial fermentation technology. According to the method, strains including lactobacillus plantarum, saccharomyces cerevisiae and bacillus subtilis are selected, the proportion of the strains is adjusted, the viable count proportion of the lactobacillus plantarum to the saccharomyces cerevisiae to the bacillus subtilis is (1-3): (1-2): (1-2), a complex microbial inoculant is formed, and pretreated roses are added for segmented fermentation. And finally, the content of water-soluble vitamins in the rose extract is increased, so that the total antioxidant activity is increased by about 35% while rose polysaccharide substances are retained. The rose extract is suitable for providing raw materials for functional food, health care products and cosmetics.

The invention discloses a preparation method and application of micromolecular oat beta-glucan, and the preparation method comprises the following steps: inoculating lactobacillus into a fermentation substrate composed of oat bran and water, and carrying out fermentation culture and sterilization to obtain the micromolecular oat beta-glucan, wherein the lactobacillus is composed of lactobacillus helveticus and lactobacillus plantarum, and the viable count ratio of the lactobacillus helveticus to the lactobacillus plantarum is 1: (0.5-2). The lactobacillus fermentation is utilized to improve the utilization rate of the oat bran and reduce the loss of active ingredients, so that the preparation method has the advantages of low cost, low energy consumption, environmental friendliness and the like, and can be directly used as an additive for preparing facial masks, essence or toner; the prepared product is superior to a product prepared by a traditional method in the aspects of DPPH free radical scavenging capacity, total antioxidant capacity, cytotoxicity, total sugar content, anti-inflammatory performance, molecular weight and the like, and has a good market application prospect.

The invention provides a pine pollen fermentation product with anti-wrinkle and anti-aging functions as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. The pine pollen extract is prepared by fermenting lactobacillus plantarum, the total polysaccharide content of the obtained pine pollen fermentation product is maximally increased by 77.3%, the FRAP total antioxidant capacity is maximally increased by 2.85 times compared with that of the unfermented pine pollen fermentation product, the anti-wrinkle effect of human skin cells can be effectively improved, and the pine pollen extract has a relatively strong proliferation effect on an ultraviolet damage model of human skin fibroblasts; the pine pollen extract has the advantages that the pine pollen extract is fermented, the elastin content is remarkably increased, the life of nematodes is effectively prolonged, lipofuscin deposition is reduced, and compared with unfermented pine pollen extract, the anti-aging capacity of the pine pollen can be effectively improved; the preparation method provided by the invention is low in price and simple in process, and the active ingredients and the efficacy of the traditional Chinese medicine composition are improved in a simplest and effective manner.

The invention discloses MnO2 (at) ZIF-67 composite nanoparticles with high oxidase activity as well as a preparation method and application of the MnO2 (at) ZIF-67 composite nanoparticles. The preparation method comprises the following steps: uniformly mixing a potassium permanganate aqueous solution and a cerous nitrate aqueous solution to obtain a mixed solution I; and adding nitric acid into the mixed solution I, carrying out hydrothermal reaction to obtain a substance MnO2, uniformly mixing the obtained MnO2 and a methanol solution of PVP, adding cobalt nitrate hexahydrate and 2-methylimidazole, and standing to obtain the MnO2 ZIF-67 composite nanoparticles. The nano-particles with high enzymatic activity are prepared through a simple hydrothermal synthesis method, have good oxidase mimetic activity, catalyze O2 and TMB to rapidly develop color and become dark blue, and can specifically, sensitively and rapidly detect the total antioxidant capacity of cells, so that the nano-particles have good practical application value.

The invention belongs to the technical field of plant breeding in molecular biology, and provides a cotton GhVIP1 gene and application thereof in drought resistance and salt tolerance of plants. The nucleotide sequence of the GhVIP1 gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the GhVIP1 gene is as shown in SEQ ID NO: 2. The gene is obviously up-regulated under the induction of salt and drought stress. An arabidopsis thaliana homozygous line overexpressed with the GhVIP1 gene is obtained through a transgenic technology, under the stress of drought and salt, the seed germination rate of the arabidopsis thaliana overexpressed with the gene is higher than that of a wild type, the growth condition of a transgenic line in the seedling stage is better than that of the wild type, the stem length, the total antioxidant capacity and the chlorophyll content are all superior to those of the wild type, and the MDA content is lower than that of the wild type; further, the drought resistance and the salt resistance of the arabidopsis thaliana are improved. When the gene is applied to drought-resistant and salt-resistant breeding of plants, the drought resistance and salt resistance of the plants can be improved, and an effective alternative scheme is provided for molecular breeding of drought-resistant and salt-resistant crops.

The invention discloses a preparation method of ganoderma lucidum mutant strain active polysaccharide and application of the ganoderma lucidum mutant strain active polysaccharide in oxidation resistance, and relates to the technical field of biology. The ganoderma lucidum mutant strain active polysaccharide is prepared by taking mycelium of a ganoderma lucidum mutant strain CGMCC No.23283 as a raw material and adopting a water extraction and alcohol precipitation method. The invention develops a novel ganoderma lucidum active polysaccharide, and a ganoderma lucidum strain adopted by the ganoderma lucidum active polysaccharide is cultured by a space mutation technology. Compared with common ganoderma lucidum strain polysaccharide, the polysaccharide provided by the invention is more prominent in antioxidant activity, and is more excellent in key indexes such as total antioxidant capacity and free radical scavenging. The invention provides a new biological material resource for the preparation of antioxidant products.

The application discloses a bacillus licheniformis YL-001 with anti-inflammatory and antibacterial effects and an application thereof, and belongs to the technical field of biotechnology. The bacillus licheniformis YL-001 is separated from soil. The preservation number of the bacillus licheniformis is GDMCC No.66556. The metabolic product of the bacillus licheniformis YL-001 provided in the application has the abilities of significantly inhibiting secretion of inflammatory factors and growth of streptococcus pneumoniae and hemolytic streptococcus b, and can improve the total antioxidant capacity of serum. The strain has good biocompatibility and high safety, has important significance for development of anti-inflammatory or antibacterial drugs, health products and food, and is especially suitable for preparation of products with throat-protecting effects.

The invention belongs to the technical field of biology, and particularly relates to acetylated raffinose and application thereof. According to the invention, acetylation modification is carried out on raffinose by using an acetylation reagent to obtain acetylated raffinose, the acetylated raffinose can significantly improve the scavenging ability and total antioxidant ability of raffinose on DPPH free radicals, and the scavenging ability on hydroxyl free radicals and the reduction ability on iron ions are also improved to a certain extent. The acetylated raffinose provided by the invention has potential application value in development of functional foods and antioxidants.

The present disclosure provides a method for processing coffee waste, the method comprises (i) subjecting an aqueous mixture comprising particulated coffee waste and an aqueous medium to hydrothermal conditions, to obtain a coffee waste slurry including particulates; and (ii) separating at least part of the particulates from said coffee waste slurry, to obtain a coffee waste product; wherein, said hydrothermal conditions comprise a temperature above 170° C.; and a pressure equal or above 1 MPa. Further provided by the present disclosure is a coffee waste product comprising in an aqueous sample volume of 1 liter any one or combination of the following characteristics: total antioxidant capacity from about 6000 and about 8000 mg Fe(II) eq., total phenolic content from about 2000 and about 3000 mg gallic acid (GA) eq., and total furfural compounds content from about 1000 ppm and 2000 ppm. Finally, the presently disclosed subject matter provides a method comprising applying to a plant or a plant soil an amount of disclosed coffee waste product.

To provide methods for producing aflibercept with reduced amount of aflibercept variants.SOLUTION: Disclosed is a method for producing aflibercept with a reduced amount of aflibercept variants expressed in a host cell cultured in a chemically defined medium (CDM), where the target value of aflibercept variants can be obtained when the cumulative concentration of iron in the CDM is about 55.0 μM or less, the cumulative concentration of copper in the CDM is about 0.8 μM or less, the cumulative concentration of nickel in the CDM is about 0.4 μM or less, the cumulative concentration of zinc in the CDM is about 56.0 μM or less, the cumulative concentration of cysteine in the CDM is about 10.0 mM or less, and / or when any single anti-oxidant is added in the CDM the cumulative concentration is about 0.001 mM to about 10 mM, and when a plurality of anti-oxidants are added the cumulative concentration of total antioxidants is about 30 mM or less.SELECTED DRAWING: Figure 1

The present invention relates to a multicolor detection kit and digital quantification method for the total antioxidant capacity of beverages, belonging to the field of chemical analysis and testing. The present invention establishes a linear correlation between the hue H value and the total antioxidant capacity of antioxidants. By combining the color development and discoloration process of a color developer, the color development results are collected using digital imaging devices such as mobile phones. The hue H value obtained by digital imaging enables rapid determination of the antioxidant capacity of beverages. The present invention develops a novel multicolor development kit that facilitates digital quantification of total antioxidant capacity, establishing a more efficient and intuitive evaluation method for the quality assessment of the antioxidant capacity of beverages.

The invention relates to a preparation method of ultrathin two-dimensional cobalt oxide rich in oxygen vacancies and an application of the ultrathin two-dimensional cobalt oxide rich in oxygen vacancies in food total antioxidant capacity detection, and belongs to the fields of low-dimensional nano materials, biological mimic enzyme catalysis and analytical chemistry. According to the preparation method, cobalt acetylacetonate and hexadecyl trimethyl ammonium bromide are adopted as raw materials, and the ultrathin two-dimensional cobalt oxide nano-enzyme rich in oxygen vacancies is prepared through a hydrothermal reaction and high-temperature pyrolysis. The obtained ultrathin two-dimensional cobalt oxide nano-enzyme rich in oxygen vacancies has excellent peroxidase-like catalytic activity and selectivity. The oxygen-vacancy-rich metal oxide has the advantages of high stability, easy mass production and low preparation cost, and is expected to be widely used in the fields of biosensing, agriculture, food engineering, catalysis and the like.

The application is suitable for the field of analog enzyme technology, and provides a light-controlled zein-based dual-enzyme-activity analog enzyme and a preparation method thereof.The preparation method comprises the following steps: under water bath conditions, dissolving zein in an alkaline aqueous solution and incubating; adding a hydroxylamine solution to the solution obtained in step S1 and continuing to react; and after the reaction is completed, obtaining the light-controlled zein-based dual-enzyme-activity analog enzyme.The application uses zein as a raw material, is modified by hydroxylamine, does not need any metal component to participate, has light-controlled switchable dual-enzyme activity, exhibits oxidase-like activity under visible light irradiation, presents peroxidase-like activity in dark conditions, and can realize reversible switching of the two enzyme activities by regulating light, effectively overcomes the problems that traditional artificial analog enzymes depend on metal ions, multiple enzyme activities coexist, interfere with each other and are difficult to accurately control, can be used for colorimetric detection of total antioxidant capacity, can be used for colorimetric detection of glucose in cascade reaction with glucose oxidase, has the advantages of simple operation, rapid response, high sensitivity, good selectivity, low cost and the like, and has a good application prospect in the fields of food analysis, clinical diagnosis and the like.

The invention provides a method for detecting the total antioxidant capacity of saliva based on iron-based MOF nano-enzyme, and belongs to the field of biosensing. The method comprises the following steps: obtaining an MIL-53Fe nano enzyme; the preparation method comprises the following steps: mixing a diluent of MIL-53Fe nano-enzyme, a sodium acetate buffer solution, 3, 3 ', 5, 5'-tetramethyl benzidine and hydrogen peroxide for reaction, adsorbing a reaction solution by using filter paper, drying and curing by using a chitosan solution to obtain the visual microfluidic detection paper-based chip. Establishing a standard curve of the chromatic value and the ascorbic acid concentration; dropwise adding the treated saliva sample onto the paper-based chip, standing for reaction to obtain a chromatic value, and calculating according to the standard curve to obtain the total antioxidant capacity value of the saliva. Complex matrix interference is broken through, the detection sensitivity and portability are improved, and therefore clinical application of saliva TAC detection in noninvasive health monitoring is promoted.

The invention belongs to the technical field of electrochemical detection, and particularly provides a sensor for evaluating oxidation resistance and a preparation method thereof, and the sensor adopts an AuNPs / PtNPs / CFME electrode formed by modifying a carbon fiber microelectrode through electro-deposition of AuNPs and PtNPs as a working electrode. The invention further provides an anti-oxidation capability evaluation method based on the sensor. According to the sensor provided by the invention, the specific surface area is obviously increased, the surface activity of the electrode is enhanced, the contact opportunity between the electrode and a substance in a solution is improved, and the electron transfer rate is favorably improved. The sensor is used for evaluating the oxidation resistance, the stability and the anti-interference performance are excellent, the electrochemical response sensitivity is relatively high, high-sensitivity quantitative detection of guanine can be realized, and the total oxidation resistance evaluation of ascorbic acid (AA) and anchusa in different producing areas is successfully realized by adopting the constructed method; and a new view angle and a new method are provided for evaluating the oxidation resistance of the plant.

The invention discloses bacillus licheniformis YL-001 with anti-inflammatory and antibacterial effects and application of the bacillus licheniformis YL-001, and belongs to the technical field of biology, and the bacillus licheniformis YL-001 is separated from soil. The preservation number of the bacillus licheniformis strain is GDMCC (China General Microbiological Culture Collection Center) No.66556. A metabolite of the bacillus licheniformis YL-001 provided by the invention has obvious capabilities of inhibiting secretion of inflammatory factors and inhibiting growth of streptococcus pneumoniae and beta hemolytic streptococcus, and can improve the total antioxidant capacity of serum, and the strain is good in biocompatibility, high in safety and suitable for industrial production. The method has important significance for developing anti-inflammatory or bacteriostatic medicines, health care products and foods, and is particularly suitable for preparing products with the sore-throat relieving effect.

The application relates to the field of analytical detection technology, and discloses a bimetallic MOF nano-enzyme as well as a preparation method and application thereof. The bimetallic MOF nano-enzyme comprises iron, cerium and an organic skeleton, the organic skeleton is 1,3-benzenedicarboxylic acid, and the iron and the cerium replace H atoms in carboxyl groups of the organic skeleton. The preparation method comprises the following steps: dissolving FeCl3.6H2O and Ce(SO4)2.4H2O in a solvent to obtain solution A; dissolving 1,3-benzenedicarboxylic acid in a DMF solution to obtain solution B; and mixing and reacting the solution A with the solution B to obtain the bimetallic MOF nano-enzyme. The bimetallic MOF nano-enzyme is used for quantitative detection of total antioxidant capacity, glutathione, ascorbic acid and cysteine, has the advantages of high stability, high reliability, high sensitivity, good selectivity, simple operation and the like, and can be widely applied in the fields of biomedicine, food safety, environmental monitoring and the like.

The present invention discloses an Aspergillus oryzae JAAS-32 and use thereof in preparation of a larval Hermetia illucens paste protein peptide. The Aspergillus oryzae JAAS-32 was deposited in Guangdong Microbial Culture Collection Center (GDMCC) on Aug. 10, 2023 with an accession number of GDMCC No: 63725, classified and named Aspergillus oryzae. A larval Hermetia illucens paste is subjected to enzymatic fermentation by the Aspergillus oryzae JAAS-32 provided by the present invention for 20-40 hours. The content of the protein peptide (<10 kDa) is 1.53 times that of an untreated group; the content of free amino acids is 1.41 times that of the untreated group; the total antioxidant activity is 1.35 times that of the untreated group. Diameters of inhibition zones against Staphylococcus aureus, Escherichia coli, and Vibrio alginolyticus are 1.62, 1.17, and 1.45 times those of the untreated group, respectively. The strain is cultured readily, fast in growth, and well-adapted. A low-cost, easy-to-operate, and efficient method for enhancing antibacterial performance of the larval Hermetia illucens paste can be established on the basis of the strain.

The application discloses a preparation method of high-quality bran raw materials, comprising the following steps: 1) washing, drying, powdering, sterilizing and reserving wheat bran; 2) adding edible fungus seed liquid into the wheat bran and uniformly mixing and fermenting to prepare high-quality bran raw materials. The application also discloses the high-quality bran raw materials obtained by the preparation method and application thereof. After fermentation, the water absorption index, water-solubility index and swelling degree of the bran are significantly increased, wherein the water-solubility index is increased by 277% at most, the total phenol content is 5.91 times of that of the unfermented sample at most, the alkyl resorcinol content is 1.55 times of that of the unfermented sample at most, and the total antioxidant capacity of the raw material is improved to 311.23 U / g, which is 5.73 times of that of the unfermented sample. Meanwhile, the fermented raw material generates alcohol aroma substance 4-ethyl guaiacol, which further improves the quality of the raw material.

Disclosed herein are a non-bee made honey formulations comprising fructose in an amount from about 20% to about 55% w / w; glucose in an amount from about 20% to about 40% w / w; one or organic acids in an amount from about 0.001% to about 5% w / w; water; and one of the following: one or more polyphenols in a concentration of at least 0.4 mM; or a total antioxidant capacity of at least 1,500 nM / uL Trolox Equivalents. In some embodiments, the total antioxidant capacity is at least that of natural bee-made clover honey. In some embodiments, the total antioxidant capacity exceeds that of natural bee-made clover honey.

The application discloses application of metformin in prolonging egg laying time of pigeons, and the metformin prolongs the egg laying time of the pigeons through a glycolysis pathway, and the mass ratio of a dosage of the metformin to a body weight of the pigeons is 60-65 mg:1 kg. It is found through experiments that the metformin can improve oxidative stress, effectively improve total antioxidant capacity in pigeon serum, reduce contents of H2O2 and MDA in the serum, rapidly improve production performance of the pigeons, has effects of promoting nutrient absorption of the body, rapidly recovering damaged functions of the body, and synergistically exerting antioxidation, and simultaneously has advantages of easy absorption of the body, improvement of bioavailability of the medicine and the like.

The invention relates to the technical field of microbial fermentation, in particular to a camellia leaf and ganoderma lucidum mixed extract and a preparation method and application thereof. According to the method, fresh camellia leaves and ganoderma lucidum mycelia are co-cultured through a two-way fermentation technology, cellulase generated by ganoderma lucidum is used for decomposing the camellia leaves, and dissolution of active substances such as flavonoids and polyphenols is promoted, so that the extraction efficiency is improved, the DPPH removal rate of a product reaches 95% or above, the total antioxidant capacity reaches 1.9 mmol / L or above, and the camellia leaves can be well applied to cosmetics and have good application prospects. The excellent skin care and anti-aging effects are realized. The bottleneck of single raw material extraction or physical mixing is broken through, through the synergistic effect of bidirectional fermentation and gradient extraction, deep integration of camellia leaf and lucid ganoderma active ingredients is achieved, and the oxidation resistance and market applicability are remarkably superior to those of the prior art.

The invention discloses a cerium-based polyoxometallate / metal organic framework-based composite nano-enzyme as well as a preparation method and detection application of the cerium-based polyoxometallate / metal organic framework-based composite nano-enzyme. The composite material is prepared by reacting ferric chloride, cerium-based polyacid and 2-aminoterephthalic acid, and has excellent peroxidase-like activity, stability and substrate affinity. Based on the material, the invention further develops a recyclable paper-based detection device, and the paper-based detection device is matched with a smart phone color recognition system to construct a rapid colorimetric detection platform for the antioxidant. The platform can realize high-sensitivity and high-selectivity detection of various antioxidants such as ascorbic acid, glutathione, gallic acid and the like, and is successfully applied to total antioxidant capacity analysis of actual samples such as fruits and the like. Compared with a traditional method, the method has the advantages of being rapid in detection, easy and convenient to operate, low in cost, environmentally friendly and the like, and is suitable for the field of food safety on-site rapid monitoring.

The invention belongs to the technical field of fruit wine processing, and particularly relates to wolfberry and green plum compound fruit wine and a preparation method thereof. The invention provides a preparation method of Chinese wolfberry fruit and green plum compound fruit wine, which comprises the following steps: performing primary fermentation on green plums subjected to pretreatment and sugaring treatment to obtain green plum raw wine, and performing secondary fermentation on green plum residues generated in the primary fermentation and Chinese wolfberry fruits to obtain green plum and Chinese wolfberry fruit compound raw wine, and finally, uniformly mixing the green plum raw wine and the green plum and Chinese wolfberry fruit composite raw wine, and performing post-treatment, ageing and refining to obtain the Chinese wolfberry fruit and green plum composite fruit wine. A two-round fermentation process of'sugaring main fermentation-low-temperature synergistic fermentation 'is adopted, so that the content of anthocyanin, the content of flavone, the content of total phenols and the total oxidation resistance in the composite fruit wine are greatly improved; finally, the compound fruit wine which is elegant in color and luster, coordinated in flavor and remarkably higher in anthocyanin content, antioxidant activity and the like than single-raw-material fruit wine is obtained.

In this invention Total Antioxidant Capacity (TAC) is determined by a combined Spectrophotometric and Electrochemical method. A reversible spectroelectrochemical sensor consist of a covalently immobilized organic ligand to indium tin oxide (ITO) coated glass, for the binding of metal cations. A metal ion binding to an organic ligand and an auxiliary ligand gives a colored complex whose color depends on the oxidation state of the metal ion. Due to the antioxidative action of the biological sample, the metal ion is reduced in proportion to the TAC of the biological sample. The change in absorption of a thin layer on ITO glass is measured with an optical detector, based on the principle of total internal reflection. The sensor is returned to its oxidized state using electrochemical oxidation. Measuring the current required for the oxidation of reduced ions, an electrochemical signal proportional to the TAC of sample is obtained.

The invention relates to the technical field of animal growth regulation, in particular to application of all-trans retinoic acid in regulation of oxidation resistance and digestion and metabolism capacity of Ningxiang pigs. According to the application, all-trans-retinoic acid is added into the Ningxiang pig feed, and the research on the antioxidant capacity of intestinal microorganisms and serum of the Ningxiang pig feed shows that all-trans-retinoic acid can improve the antioxidant capacity of the Ningxiang pig by enhancing the total antioxidant capacity of the Ningxiang pig, changing the diversity of intestinal flora of the Ningxiang pig and changing microbial metabolites and metabolic pathways of the intestinal flora; the biosynthesis and metabolism of amino acids are promoted, so that the oxidation resistance and digestion and metabolism capabilities of the Ningxiang pigs are enhanced.

The invention discloses a capsule medicine for treating hyperlipemia as well as a preparation method and application thereof, and belongs to the technical field of hot-dip galvanized steel plates, the capsule medicine is prepared from the following raw materials in percentage by weight: 58-69% of egg extract, 8.3-10.3% of tea polyphenol, 13.2-18.6% of sophora flavescens extract and 7.1-13.1% of turmeric extract. Through the mode, the egg extract improves antioxidant indexes of liver VLDL, superoxide dismutase, glutathione peroxidase, total antioxidant capacity and the like and reduces accumulation of plasma triglyceride, total cholesterol and the like, the tea polyphenol and the sophora flavescens extract are beneficial for improving the activity of the egg extract, the effect of the egg extract is further improved, and meanwhile, the effect of the egg extract is further improved. In addition, the tea polyphenol can inhibit the generation of fat and effectively reduce energy accumulation, and in addition, the turmeric extract is beneficial to accelerating the rapid discharge of triglyceride and total cholesterol after removal, so that the treatment of hyperlipidemia is realized.

According to the radix isatidis enzymolysis polysaccharide with the antioxidant activity and the preparation method thereof, the enzymolysis process is optimized through a single factor experiment and a response surface method, the optimal enzymolysis conditions (the enzyme activity is 120 U / mL, the enzymolysis temperature is 70 DEG C and the enzymolysis time is 6 hours) are determined, and the prepared enzymolysis polysaccharide can effectively remove DPPH and ABTS free radicals, reduce iron ions and improve the antioxidant activity of radix isatidis. The isatis root polysaccharide has good antioxidant activity, is non-toxic to cells, can significantly improve the total antioxidant capacity in cells and reduce the MDA content, shows good cell antioxidant activity, is simple and feasible in preparation process, and provides a scientific basis for development and application of isatis root polysaccharide.

The invention discloses a lactobacillus rhamnosus CQFP202457 and application of the lactobacillus rhamnosus CQFP202457 and ellagic acid in relieving exercise fatigue, and belongs to the technical field of microorganisms. The preservation number of the lactobacillus rhamnosus CQFP202457 is CGMCC (China General Microbiological Culture Collection Center) No. 29784. The composition provided by the invention contains ellagic acid and lactobacillus rhamnosus CQFP202457, and can significantly prolong the exercise exhaustion time, reduce the content of fatigue-related metabolites in serum, improve the total antioxidant ability of serum, liver and skeletal muscle and the antioxidant enzyme gene expression level, and improve the exercise-induced liver and skeletal muscle tissue damage. The strain and the composition thereof provided by the invention can be used as exercise synbiotics for preparing products for relieving exercise-induced fatigue, improving exercise performance, reducing exercise-induced oxidative stress or relieving exercise-induced muscle injury.