12 results about "Proteolytic enzymes" patented technology

The present invention provides, among other things, a masked cytokine comprising an interleukin 2 (IL-2) polypeptide, a VHH masking moiety, an anti-PD1 targeting moiety, and an engineered Fc domain comprising a tumor-associated protease cleavage site. In such masked cytokine, the IL-2 polypeptide is engineered to be activatable by a protease at a target site, such as in a tumor microenvironment. The VHH masking moiety blocks, occludes, inhibits (e.g., decreases) or otherwise prevents (e g masks) the activity or binding of the cytokine to its cognate receptor or protein. Upon proteolytic cleavage of the cleavage site in the Fc domain, the IL-2 polypeptide becomes activated, which renders it capable of binding to its cognate receptor or protein with increased affinity.

The present application provides the use of proteolytic enzymes to reduce or eliminate interfering antibodies in immunoassays for antigens and / or antibodies. The present disclosure utilizes biological methods or biological methods in combination with chemical methods to disrupt the structure of interfering antibodies, thereby attenuating or even eliminating the signal (false positive) or masked signal (false negative) from interfering antibodies, which can significantly improve the accuracy of reagent detection.

Provided is a method for measuring glycated hemoglobin in a sample containing hemoglobin and plasma, the method including reacting glycated hemoglobin in a sample containing hemoglobin and plasma, a proteolytic enzyme, and a fructosyl peptide oxidase to generate hydrogen peroxide, and measuring the generated hydrogen peroxide, wherein the reaction is performed in the presence of ferrocyanide and a ferrocene derivative.

Use of caseinolytic protease P (ClpP) function and / or concentration as a biomarker for predicting the response of a neoplastic disease, preferably cancer or another disease where enhancing ClpP activity may provide a therapeutic benefit, to a compound of Formula I. In other aspects it relates to methods and kits, as well as methods of treatment involving the use of the biomarker.

The present invention relates to a novel peptide comprising the amino acid sequence of SEQ ID NO: 1, and an antibacterial composition comprising same. The peptide of the present invention, which is an Enterococcus lactis-derived antibacterial peptide APP101, has specific antibacterial activity against periodontitis-causing bacteria including Porphyromonas gingivalis, Fusobacterium nucleatum, Enterococcus faecalis, Streptococcus parasanguinis, and Streptococcus salivarius, and has stability against proteases, pH, and salt concentrations. The antimicrobial composition of the present invention can be usefully employed for the prevention or treatment of periodontitis.

FIELD: veterinary science; animal husbandry; feed production.SUBSTANCE: method for feeding laboratory and decorative rodents. The method is characterized by the fact that extruded feed with an additive is introduced into the animals’ diet for at least 28 days, and the additive used is a protein hydrolysate of Hermetiaillucens larvae in an amount of 0.5% of the feed weight. The protein hydrolysate of Hermetiaillucens larvae is obtained by enzymatic hydrolysis using proteolytic enzymes, dried and introduced into extruded feed as a powder.EFFECT: improving the absorption of feed and the condition of the coat.1 cl, 3 tbl, 3 ex

This application relates to a method, kit, and application for detecting IgM antibodies. The method includes the following steps: mixing a hydrolysis reagent containing a proteolytic enzyme and an immunoassay reagent capable of specifically binding to the target IgM antibody in the test sample with the test sample to obtain a test reaction solution; detecting the signal value of the reaction solution; wherein the proteolytic enzyme can destroy the structure of interfering proteins in the test sample. The solution provided in this application can reduce or eliminate the interference of matrix effects produced by interfering proteins, thereby improving the detection accuracy of IgM antibodies.

The present invention relates to a method for producing a composition comprising a solid carrier, a proteolytic enzyme or fragment thereof immobilized on the surface of the solid carrier, a protective layer protecting the proteolytic enzyme or fragment thereof by embedding it, and a functional component immobilized on the surface of the protective layer, wherein the functional component immobilized on the surface of the protective layer is a polymer comprising repeating units, each containing at least one amino group and / or at least one thiol group. The present invention also relates to a method for producing the composition.

The present invention is directed to novel compositions for treating cancer. The present invention relates to novel compositions comprising proteolytic enzymes and clarifying agents and methods of using these compositions to treat and / or prevent cancer.

The present application relates to the field of biomedical sensing and flexible electronic technology, and particularly relates to a protease-responsive sensor, a battery-free multiplexed wound monitoring patch and a preparation method and application thereof.The sensor is a protease-responsive photonic crystal hydrogel, which comprises silica nanoparticles and methacrylated gelatin in a mass ratio of 500-600:30-90.The battery-free multiplexed wound monitoring patch comprises the protease-responsive sensor.The protease-responsive sensor of the present application utilizes PCH to specifically respond to protease, and the PCH is prepared by a one-step method of colloidal self-assembly-in-situ photopolymerization.The monitoring patch of the present application can simultaneously monitor four indexes of protease activity, pH, glucose and D-lactic acid in the same patch, and can realize the combined evaluation of the wound without electrodes, thereby improving the accuracy of infection judgment.

The application discloses a soil staphylococcus with high-temperature resistance and smoking resistance, and application thereof, and belongs to the technical field of microbial application and fermented meat products. The soil staphylococcus has a preservation number of CGMCC No. 34994, is named XUCSB 058, has nitrate reductase, catalase, proteolytic enzyme and lipolytic enzyme, can produce acid by utilizing glucose, and has the characteristics of acid resistance, salt resistance, nitrite resistance, low-temperature resistance, non-hemolysis, non-stick production, non-gas production, non-biogenic amine production, non-NH3 production, non-H2S production and the like. The soil staphylococcus has the characteristics of high-temperature resistance and smoking resistance, can promote the persistent formation of color and flavor of smoking fermented meat (such as bacon, sausage, smoked ham and the like), and has the potential of being safely used in the production of various smoking fermented meat (such as bacon, sausage, smoked ham and the like).