12 results about "Demineralized bone matrix" patented technology

The invention relates to a functionalized cartilage tissue repair material as well as a preparation method and application thereof, and belongs to the technical field of tissue engineering. The invention aims to solve the problems that the existing decalcified bone matrix is insufficient in cartilage induction in cartilage repair, and is easy to cause blood vessel ingrowth and fibrosis repair. The preparation method comprises the following steps: inoculating cartilage cells on a three-dimensional porous frame structure carrier, carrying out in-vitro dynamic pre-culture to enable the cartilage cells to secrete an extracellular matrix, and carrying out decellularization treatment to obtain the functionalized cartilage tissue repair material retaining the cartilage specific matrix. The material has a suitable pore structure and abundant II-type collagen deposition, can effectively promote chondrocyte differentiation and hyaline cartilage regeneration, inhibits blood vessel ingrowth, maintains a cartilage vascular-free microenvironment, significantly improves the structure and function reconstruction effect of cartilage defect repair, and has good biocompatibility and clinical transformation potential.

An enhanced osteoinductive composition is prepared by subjecting particulate bone to a demineralization process that is optimized based on the particle size. In broad particle size ranges, ground bone is separated by particle size and each size range is subjected to independent demineralization conditions that maintain high levels of endogenous growth factors found in bone. Following demineralization, the size ranges are recombined to create an enhanced osteoinductive composition. In narrow particle size ranges, bone is milled to achieve a predetermined size, and the demineralization process is adjusted to this size range. Size-optimized demineralization is also used to create enhanced demineralized bone matrix particles in the size range of <125 μm. Enhanced osteoinductive compositions using size-optimized demineralization bone matrix and protein isolation and / or bone gelatin creation techniques are disclosed. Tissue repair compositions using the enhanced osteoinductive particles are also disclosed.

An apparatus for mixing bone material with a liquid is provided. The apparatus includes a chamber having a proximal end and a distal end and bone material disposed within the chamber, the bone material comprising DBM particles of milled and lyophilized demineralized bone fibers; and a plunger having at least a portion slidably disposed within the proximal end of the chamber and configured to dispense the bone material mixed with the liquid from the distal end of the chamber when the plunger is in an extended position.

The invention belongs to the technical field of biomedical materials, and discloses a bone tissue repair material for promoting vascularization and new bone generation and a preparation method thereof. The bone tissue repair material is composed of a decalcified bone matrix and a degradable biological source gel material. The repair material has a loose porous three-dimensional structure similar to that of natural cancellous bone, and the temperature-sensitive gel material filled in the pore structure of the repair material can form gel in situ in vivo and is attached to pores of a decalcified bone matrix, so that cell migration, adhesion and proliferation can be promoted, the degradation rate of gel in vivo can be reduced, and the repair material has a good application prospect. The continuous release of the pro-angiogenesis factor is ensured. Good biocompatibility is achieved, and cytotoxicity and immunogenicity are avoided; meanwhile, in the slow degradation process, angiogenesis promoting factors are continuously released for a long time, and angiogenesis and new bone generation are promoted. Therefore, the implanted biological scaffold prepared from the bone tissue repair material has excellent bone repair and regeneration promotion capability and wide application prospect.

An enhanced osteoinductive composition is prepared by subjecting particulate bone to a demineralization process that is optimized based on the particle size. In broad particle size ranges, ground bone is separated by particle size and each size range is subjected to independent demineralization conditions that maintain high levels of endogenous growth factors found in bone. Following demineralization, the size ranges are recombined to create an enhanced osteoinductive composition. In narrow particle size ranges, bone is milled to achieve a predetermined size, and the demineralization process is adjusted to this size range. Size-optimized demineralization is also used to create enhanced demineralized bone matrix particles in the size range of <125 μm. Enhanced osteoinductive compositions using size-optimized demineralization bone matrix and protein isolation and / or bone gelatin creation techniques are disclosed. Tissue repair compositions using the enhanced osteoinductive particles are also disclosed.

The invention discloses a demineralized bone matrix fiber as well as a preparation method and application thereof. The method comprises the following steps: firstly, cleaning and disinfecting natural bones separated out of a body, then cutting the natural bones into bone blocks, further degreasing the bone blocks, and cutting the bone blocks into fibers with the length of 1-100mm and the thickness of 0.2-1.4 mm; then placing the bone fibers obtained by cutting in an acidic demineralization solution for demineralization until the calcium content is 0-20%; the method comprises the following steps: demineralizing bone fibers, then reacting with the demineralized bone fibers by using an anionic surfactant for 0.5-3 hours, cleaning, then reacting for 12-18 hours by using a nonionic surfactant, completing two-stage decellularization, cleaning, and freeze-drying for preservation. During clinical use, the demineralized bone fibers which are kept in a freeze-drying manner are mixed with hydrated liquid or mixed with one of sodium carboxymethyl cellulose, gelatin, poloxamer and chitosan to form an ointment substance which is used as a bone defect repairing material.

An enhanced osteoinductive composition is prepared by subjecting particulate bone to a demineralization process that is optimized based on the particle size. In broad particle size ranges, ground bone is separated by particle size and each size range is subjected to independent demineralization conditions that maintain high levels of endogenous growth factors found in bone. Following demineralization, the size ranges are recombined to create an enhanced osteoinductive composition. In narrow particle size ranges, bone is milled to achieve a predetermined size, and the demineralization process is adjusted to this size range. Size-optimized demineralization is also used to create enhanced demineralized bone matrix particles in the size range of <125 µm. Enhanced osteoinductive compositions using size-optimized demineralization bone matrix and protein isolation and / or bone gelatin creation techniques are disclosed. Tissue repair compositions using the enhanced osteoinductive particles are also disclosed.

The invention discloses an intelligent preparation system and method of a bone repair biological material, and relates to the technical field of biomedical materials.The system comprises a raw material pretreatment and conveying unit used for metering, pretreating and independently conveying a calcium phosphate precursor, a decalcified bone matrix, a hydrogel prepolymer, a bone growth promoting factor and PLGA; the nano-microsphere preparation and packaging unit is in communication connection with the raw material pretreatment and conveying unit and used for receiving PLGA and bone growth promoting factors and preparing PLGA nano-microspheres encapsulated with the bone growth promoting factors under the controllable condition, and the particle size of the PLGA nano-microspheres ranges from 80 nm to 500 nm; by means of accurate metering and pretreatment of the intelligent control center on multi-component raw materials, active regulation and control on the particle size of the nano-microspheres and online monitoring and feedback on the mixing reaction process, repeatable and standardized preparation of the bone repair material with the complex formula is achieved.

The utility model discloses a freeze-drying device for strip-shaped decalcified bone matrix, which comprises a drying chamber, a rack, a multi-layer sample rack, a cold trap, a compressor, a vacuum pump and a touch screen, the drying chamber is fixed at the top of the rack, the multi-layer sample rack and the cold trap are adjacently arranged in the drying chamber, and the compressor is fixed on the rack. The compressor, the vacuum pump and the touch screen are respectively arranged on the rack, the compressor is arranged close to the vacuum pump, the touch screen is arranged close to the drying chamber, a heating partition plate is further arranged at the bottom of the multi-layer sample rack, and a temperature sensor is arranged at the bottom of the heating partition plate. Efficient drying is achieved, the space utilization rate is increased through the multi-layer sample frame design, and the single-time handling capacity is greatly improved; the cold trap is directly connected with the compressor to shorten a condensation path, so that the drying period is shortened. Gradient temperature control is achieved through each layer of independent heating partition plate and a temperature sensor, and it is guaranteed that the strip-shaped gel is heated evenly, and the pore structure is complete. And the shape is fixed: the strip-shaped sample groove and the hydrophobic coating prevent gel deformation or adhesion.

A method for producing a composite demineralized bone matrix composition using a one-step process is described. The composite demineralized bone matrix composition is produced from the biologically-derived bone. In addition, the composite demineralized bone matrix composition contains bone minerals according to the original composition proportion in the bone and may provide a bone mineral content condition that is closest to that in an environment in which in vivo bone formation occurs. In addition, the composition contains a bone morphogenetic protein (BMP-2), and thus enables a stable and excellent bone formation effect to be derived.

A method of tuning mineralization of a bone matrix includes enhancing formation of carboxymethyl-lysine (CML) on the bone matrix. The demineralized bone matrix is positioned between a reservoir of a calcium solution and a reservoir of a phosphate solution. The demineralized bone matrix, the calcium solution and the phosphate solution is incubated for a period of time to enable calcium and phosphate ions to form mineral precursors and deposit on the bone matrix enhanced with CML.