31 results about "Bone particle" patented technology

The utility model relates to the technical field of enzymolysis, and provides a fishbone oligopeptide enzymolysis device which comprises a supporting frame, an enzymolysis shell is fixedly arranged at the top end of the supporting frame, a rotating shaft is rotationally arranged in the enzymolysis shell, three stirring rods are arranged on the outer side of the rotating shaft in a sleeving mode, a stirring scraping rod is fixedly arranged on the outer side of the lower portion of the rotating shaft, and the stirring scraping rod is arranged on the outer side of the lower portion of the rotating shaft. The outer side of the stirring scraping rod is attached to the inner wall of the enzymolysis shell, a feeding port is formed in the top end of the enzymolysis shell, a discharging channel is fixedly arranged at the top end of the feeding port, a smashing shell is fixedly arranged at the top end of the discharging channel, a feeding port is formed in the top end of the smashing shell, and a smashing wheel is rotationally arranged in the smashing shell; a plurality of filtering holes are formed in the inner wall of the lower portion of the smashing shell, and through the technical scheme, the problems that in the enzymolysis process of fish bones in the prior art, due to the fact that bone particles of the fish bones are large, the fish bones are not thoroughly connected with enzyme in the enzymolysis process, the enzymolysis effect is affected, and resources are wasted are solved.

1. Name of the designed product: bone crusher (electric). 2. Use of the designed product: used in the medical field for the preparation of bone particles. 3. Design points of the designed product: in the combination of shape and pattern. 4. Picture or photo best indicating the design points: perspective view 1.

One embodiment of the present invention provides a dental drill comprising: a main body fastened to a driving means so as to be rotatable in one direction; a cutting part which extends from the main body and which provides, during rotation of the main body in one direction, both a drilling function of drilling the alveolar bone and a burnishing function; and a stopper which is formed between the main body and the cutting part and which limits the drilling depth of the cutting part, wherein the cutting part includes a spiral groove which is formed on the outer surface thereof in the direction opposite to the one direction, and through which alveolar bone particles generated during drilling of the alveolar bone are discharged.

The invention belongs to the technical field of biological materials and tissue engineering, and particularly relates to a fluorine-modified collagen bone repair material as well as a preparation method and application thereof. According to the present invention, pig-derived collagen and fluorine-modified pig-derived hydroxyapatite bone particles are combined, the purpose of the present invention is to develop a novel collagenized bone repair material, the ratio of collagen to bone particles is accurately optimized, and the formula scheme capable of providing the optimal osteogenesis performance is determined, such that the repair material has characteristics of uniform biological source, good bone repair effect, and good bone repair effect. High traceability is ensured, and repair and regeneration of bone tissues can be effectively promoted.

The bone grafting device comprises a bone grafting funnel and a push rod, the bone grafting funnel comprises a funnel storage bin and a bone grafting pipe, the bone grafting pipe communicated with the funnel storage bin is of an integrated transparent structure, the middle lower portion of the side wall of the bone grafting pipe is provided with a drainage hole and scale marks, and the lower end of the bone grafting pipe is provided with an object outlet. The diameter of the push rod is matched with the inner diameter of the bone grafting tube. The intervertebral space bone grafting tube is applied to the intervertebral space bone grafting process under a spine endoscope, bone grains can be preloaded into the bone grafting tube, the push rod is used for pushing and injecting the bone grains into the intervertebral space, the drainage hole of the bone grafting tube can prevent water from flowing back and overflowing during pushing and injecting, the scale marks can accurately grasp the insertion depth of the bone grafting tube and the bone grafting body amount, and the whole process is visual. The bone grafting funnel is made of disposable transparent materials and has the advantages of being low in use cost, visible in the whole process, safe to use and high in bone grafting efficiency.

This invention discloses a biodegradable, highly bioactive magnesium bone particle, its preparation method, and its applications, belonging to the field of biomedical materials technology. Using magnesium bone particles as a matrix, an active composite coating layer comprising biodegradable polymer components and inorganic phase components is formed on its surface to construct biodegradable, highly bioactive magnesium bone particles. The biodegradable polymer components are one or more selected from polycaprolactone, polylactic acid, polylactic-co-glycolic acid copolymer, polyhydroxyalkanoates, chitosan, and their derivatives; the inorganic phase is one or more selected from hydroxyapatite, titanium dioxide, calcium phosphate materials, and combinations thereof. The preparation method includes magnesium bone particle matrix preparation, active composite layer slurry preparation, magnesium bone particle coating, and post-treatment. The prepared biodegradable, highly bioactive magnesium bone particles exhibit good degradation regulation performance and excellent bioactivity, and can be used in bone tissue engineering, bone defect repair, and scaffold filling fields.

The utility model belongs to the technical field of tooth implantation, and particularly relates to a bone meal grinder for caulking of dental implant, which comprises a handheld rod, the handheld rod is of a cylindrical structure, one end of the handheld rod is detachably provided with a positioning sleeve, and the end face of the end is provided with first grinding lines; one end of the positioning sleeve is connected with the handheld rod, a grinding head is clamped in the other end of the positioning sleeve, second grinding grains matched with the first grinding grains are arranged on one end face of the grinding head, and a connecting rod is arranged at the other end of the grinding head. The handheld rod and the connecting rod are convenient for a user to grab the grinder, gum bone particles are placed between the grinding head and the handheld rod, and the grinding head is rotated through the connecting rod, so that the first grinding lines and the second grinding lines are matched to grind the gum bone particles into powder, the working efficiency is improved, and the operation difficulty is reduced.

The invention provides a preparation method of a collagen-based bone filling material, which comprises the following steps: S1) heating an acellular matrix biological membrane for thermal crosslinking treatment, and crushing to obtain matrix powder; and S2) mixing the matrix powder with a solvent, performing whipping treatment, then adding inorganic bone particles, performing mixing, and performing freeze drying and thermal crosslinking treatment to obtain the collagen-based bone filling material. Compared with the prior art, the collagen-based bone filling material provided by the invention is composed of the acellular matrix biological membrane and the natural inorganic bone particles, and the acellular matrix biological membrane is subjected to thermal crosslinking treatment, crushing, swelling and whipping treatment to form a stable three-dimensional network structure; the bone filling material prepared by the preparation method disclosed by the invention can obviously prolong the in-vitro degradation time after being physically crosslinked with inorganic bone particles, and the bone filling material has no chemical reagent residue, not only has good biocompatibility, but also has good rebound resilience and plasticity, and is convenient to operate.

The invention relates to the technical field of bone meal injectors, in particular to a recyclable bone meal injector which comprises an injection tube, a bone particle storage tube is detachably connected into the injection tube, a sealing mechanism is arranged in the bone particle storage tube and the injection tube jointly, and the bone particle storage tube is arranged in the sealing mechanism; the bone particle storage tube is arranged at one end of the injection tube in a penetrating mode, and an injection mechanism is arranged in the injection tube. According to the bone particle injection device, the filter suction cap and the injection head can be quickly mounted and dismounted according to needs, and the bone particle storage tube can be mounted according to needs, so that bone powder, normal saline and autoblood in the bone particle storage tube can be mixed through cooperation of the filter suction cap and the bone particle storage tube; the injection position can be quickly positioned through the injection head and the bent pipe fitting so that filling can be carried out, all the components in the device can be detached according to needs, cleaning and disinfection are facilitated, and recycling is facilitated.

The lumbar vertebra posterior fusion bone grafting device comprises a vertical pipe body, an inlet is formed in the left side of the middle of the pipe body, a pressing mechanism capable of downwards moving bone grains fed into the inlet by one body section after being pressed is inserted into the pipe body, and the pressing mechanism is connected with the upper end of the pipe body through a spring for returning. Under the action of the spring, the pressing mechanism can move the bone grains fed in from the inlet by a certain distance into the tube body every time the pressing mechanism is pressed, the bone grains can be divided into one body section or multiple body sections, the next bone grain can be conveniently conveyed, and the device further comprises a pushing mechanism which is located in the pressing mechanism and can send all the bone grains loaded in the tube body into the posterior lumbar vertebra; and the pushing mechanism is used for conveying the bone grains filled in the tube body into the posterior lumbar vertebra.

The utility model relates to the technical field of noise reduction equipment, and provides a recycled aggregate porous concrete acoustic board which comprises a first concrete layer and a second concrete layer, and the first concrete layer and the second concrete layer are stacked to form an acoustic board block. The void ratio of bone grains in the first concrete layer is 10%-30%, the slump of concrete in the second concrete layer is larger than 200 mm, and the slump expansion degree ranges from 450 mm to 550 mm. According to the recycled aggregate porous concrete acoustic board, the first concrete layer and the second concrete layer are arranged, the first concrete layer is the porous concrete layer, and concrete in the second concrete layer is concrete with high fluidity, so that the acoustic board has a good sound absorption effect, and meanwhile, the dead weight of the acoustic board is reduced; the recycled aggregate porous concrete acoustic board can be suitable for various application scenes, and the applicability of the recycled aggregate porous concrete acoustic board is improved.

The present invention relates to the field of bone processing technology, and specifically to a composite device for autologous bone harvesting and bone processing, comprising: a bone harvesting auxiliary device and a bone processing device; the bone harvesting auxiliary device comprises: a bone harvesting caliper, a guide sleeve and a depth-limiting Steinmann pin; the bone processing device comprises: a bone box, a fixing assembly and a cutting assembly. The composite device for autologous bone harvesting and bone processing of the present invention can accurately harvest materials based on preoperative imaging measurement results to avoid waste of resources. During the operation, the device can obtain cancellous bone, unicoral bone, bicortical bone and tricortical bone. At the same time, bone products of various shapes and sizes, such as bone particles, bone strips, bone slices, bone blocks, etc., can be prepared according to the requirements of the bone grafting area.

The invention provides a fusion cage capable of being distracted under a mirror, and belongs to the technical field of medical instruments. The invention provides an under-mirror distraction fusion cage which comprises two fusion pieces, the upper end faces of the fusion pieces are provided with clamping bases and clamping columns, the free ends of the clamping columns are provided with clamping tooth blocks, one fusion piece is overturned by 180 degrees and buckled on the other fusion piece, the two fusion pieces are the upper fusion piece and the lower fusion piece, and the clamping tooth blocks are arranged on the clamping bases. The upper clamping column of the upper fusion piece is inserted into the lower clamping base of the lower fusion piece, the upper clamping column and the lower clamping base are arranged in a meshed mode, the upper clamping column has the freedom degree of moving up and down in the lower clamping base, the lower clamping column of the lower fusion piece is inserted into the upper clamping base of the upper fusion piece, the lower clamping column and the lower clamping base are arranged in a meshed mode, and the lower clamping column has the freedom degree of moving up and down in the upper clamping base. A bone grafting space is formed between the upper fusion piece and the lower fusion piece. The fusion cage capable of being distracted under the microscope is simple in structure, the bone grafting space is increased, the fusion cage is in a post-bone grafting mode, the number of implanted bone particles is large, and the bone fusion rate is increased.

The application relates to the field of bone powder processing, in particular to a high-efficiency crushing and grinding device special for wolf bone processing, which comprises a crushing structure, a cleaning structure and a grinding structure. The crushing structure is used for crushing wolf bone raw materials entering a crushing box. The cleaning structure comprises a feeding pipe, a metering cylinder, cleaning boxes and a tee joint pipe. A spiral feeder is rotatably arranged in the feeding pipe. Multiple groups of cleaning boxes are arranged at the bottom of the feeding pipe and correspond to the lower side of the discharge port. The metering cylinder is used for conveying wolf bone raw materials with a preset capacity into the cleaning boxes. The tee joint pipe is arranged at the bottom of the cleaning box. The side feeding port of the grinding structure is communicated with the wolf bone particle discharging ports of the multiple groups of tee joint pipes and is used for grinding the cleaned wolf bone particles, so that the problem that the existing wolf bone surface-attached meat scraps and the residual bone marrow in the bone cannot be correspondingly removed and the crushed meat scraps and bone marrow are mixed into wolf bone powder ground from the wolf bone and thus affect the quality of the wolf bone powder is solved.

The application provides a distractible fusion cage for implanting into a vertebra from a vertebral foramen, which comprises: a front support part gradually decreasing from front to back along an X direction; a rear support part having a first state and a second state, when the rear support part is switched from the first state to the second state, two branches are distracted by the front support part; a support band ring, the front end of the support band ring is limited by the front support part, and the rear end passes through the rear support part; during the conversion of the rear support part from the first state to the second state, the front support part and the rear support part relatively move close to each other, so that the rear end of the support band ring protrudes backward relative to the rear support part, and the vertebral foramen is shielded. The support band ring of the distractible fusion cage of the application protrudes backward to shield the vertebral foramen, without the need of additional occlusion instruments, so that the loss of bone particles and bone mud from the open annular fibrosus incision into the fusion cage can be blocked, the bone graft amount of the intervertebral space is ensured, and the success rate of bone fusion is improved.

The application provides a preparation method of a collagen-based bone filling material, comprising the following steps: S1) heating and performing heat crosslinking treatment on a decellularized matrix biomembrane, and then crushing the decellularized matrix biomembrane to obtain a matrix powder; S2) mixing the matrix powder with a solvent to perform whipping treatment, then adding inorganic bone particles to mix, and then performing freeze-drying and heat crosslinking treatment to obtain the collagen-based bone filling material. Compared with the prior art, the collagen-based bone filling material provided by the application is composed of a decellularized matrix biomembrane and natural inorganic bone particles, wherein the decellularized matrix biomembrane is subjected to heat crosslinking treatment, and then is subjected to crushing, swelling and whipping treatment, so that a stable three-dimensional network structure is formed; after physical crosslinking of the inorganic bone particles, the in-vitro degradation time is significantly prolonged; the bone filling material has no chemical reagent residue, has good biocompatibility, good resilience and plasticity, and is convenient to operate.

A surgical tool (10) for shaving and collecting bone particles, comprising: - a handle (20) provided with a handle (21); - a collection chamber (25) having a proximal end close to the handle (21) and a free distal end; - a stem (30) at least partially disposed within the collection chamber (25); - a blade body (40) fixed to a front end (32) of the stem (30), wherein the blade body (40) radially protrudes from the stem (30) and is axially disposed outside the collection chamber (25) in the vicinity of the distal end of the collection chamber (25); and - a spacer (50) interposed between a trailing face (42) of the blade body (40) and the distal end of the collection chamber (25), the trailing face (42) of the blade body (40) being kept at a predetermined non-zero axial distance from the distal end of the collection chamber (25) at least for a circumferential section of the spacer (50), wherein the spacer (50) defines a passage opening (500) for introducing the bone particles shaved by the blade body (40) into the collection chamber (25).

The invention relates to the field of bone meal processing, in particular to a special efficient crushing and grinding device for wolf bone processing, which comprises a crushing structure, a cleaning structure and a grinding structure, the crushing structure is used for crushing wolf bone raw materials entering a crushing box, and the cleaning structure comprises a feeding pipe, a metering cylinder, a cleaning box and a three-way pipe; a spiral feeder is rotationally installed in the feeding pipe, the multiple sets of cleaning boxes are sequentially arranged on the lower side, corresponding to a discharging opening, of the bottom of the feeding pipe, the metering cylinder conveys wolf bone raw materials with the preset capacity into the cleaning boxes, and the three-way pipe is arranged at the bottom of the cleaning boxes. A feeding hole in one side of the grinding structure is communicated with discharging holes of the wolf bone particles of the plurality of groups of three-way pipes and is used for grinding the cleaned wolf bone particles, so that the problems that minced meat attached to the surfaces of existing wolf bones and marrow remaining in the bones are not correspondingly removed, the crushed minced meat and marrow are mixed into wolf bone powder formed by grinding the wolf bones, and the quality of the wolf bone powder is improved are solved. The quality of the wolf bone powder is influenced.

The utility model discloses a heating device convenient for temperature control for skeletal grain degreasing processing, which belongs to the field of skeletal grain production, and comprises a heating cylinder, a heating groove is arranged in the heating cylinder, one side of the heating cylinder is fixedly connected with a driving motor, an output shaft of the driving motor is fixedly connected with a conveying auger, and the conveying auger is fixedly connected with the heating cylinder. The conveying auger is located in the heating groove, the conveying auger is of a shaftless type, and a temperature control assembly used for controlling the temperature is arranged outside the heating barrel; according to the heating device convenient for temperature control and used for skeletal grain degreasing processing, the spiral heating pipe is mounted in the inner wall of the heating cylinder, and supply of circulating heating water is realized through the temperature control water tank and the water pump, so that the temperature in the heating cylinder can be more accurately controlled. By means of the design, the problem of uneven temperature caused by a traditional direct heating mode is avoided, it is guaranteed that the bone grains are evenly heated in the degreasing process, and the degreasing effect and the product quality are improved.

The utility model relates to a deproteinization component and a deproteinization device, and relates to the field of deproteinization equipment. The deproteinization component comprises a screening barrel, a baffle and a rotating part, the baffle is rotationally connected to the interior of the screening barrel and rotates around the horizontal axis of the baffle, the outer circumferential wall of the baffle is attached to the inner circumferential wall of the screening barrel, and the rotating part is connected with the baffle and used for driving the baffle to rotate. In the deproteinization process, bone particles to be deproteinized can be contained in parts by the deproteinization component, stacking of the bone particles is reduced, and the efficient deproteinization effect can be achieved under the condition that stirring is not conducted.

A method for stabilizing a vertebral body is provided. The method includes creating one or more openings into a central region of the vertebral body and dispose at least one cannula in the one or more openings. The method includes forming an internal cavity within the vertebral body through at least one of the one or more openings. The method includes injecting bone particles into the internal cavity of the vertebral body through a bone particle catheter disposed within the at least one cannula. The method includes applying pressure to the bone particles within the internal cavity of the vertebral body, thereby compressing them against a sidewall of the internal cavity. The method includes removing the at least one cannula and closing the one or more openings, thereby sealing the bone particles within the internal cavity of the vertebral body. A system for stabilizing a vertebral body is also provided.

The invention relates to the technical field of crushing equipment, in particular to bone crushing equipment for bone grafting operation suitable for spine surgery. A bone crushing cavity is formed in the bone crushing machine, a bone crushing block is slidably connected to the bottom of the bone crushing cavity, and bone crushing teeth are arranged on the bone crushing block in the moving direction of the bone crushing block and the side wall of the bone crushing cavity in an array mode; an eccentric power mechanism and a pulse mechanism are arranged in the bone crushing block, the eccentric power mechanism is used for driving the bone crushing block to reciprocate, and the pulse mechanism is used for generating pulse jet flow at the bottom of the bone crushing cavity based on movement of the bone crushing block; a filtering mechanism used for filtering bone particles is further arranged on the side wall of the bone crushing cavity, a preset distance is formed between the filtering mechanism and the bottom of the bone crushing cavity, and the filtering mechanism communicates with a collecting mechanism used for collecting the bone particles. According to the invention, autologous bones can be efficiently treated, and high-quality bone particles with uniform sizes can be prepared.

The present application relates to a kind of mesoscopic modeling and analysis method of concrete structure, comprising the following steps: step 1) according to the size characteristic parameter of concrete component, determine lattice node coordinates, and connect lattice node to obtain the frame of lattice model, wherein the lattice model includes lattice node and the lattice unit connected between lattice node each other;Step 2) according to the grading of each component material used by concrete material, determine the particle size and quantity of coarse aggregate and the mesoscopic two-dimensional parameter of each component of concrete;Step 3) generate coarse bone particle mesoscopic random distribution model based on random distribution algorithm, the particle size and quantity of coarse aggregate, and according to the mesoscopic random distribution model of coarse aggregate, give the corresponding material attribute and element attribute of lattice unit, obtain lattice model;Step 4) import lattice model into ABAQUS secondary development platform, and carry out load calculation.Compared with prior art, the present application comprehensively considers the mesoscopic structure characteristics of concrete material, and provides a refined numerical simulation idea.

The utility model discloses a bone grafting set device for minimally invasive spinal surgery, which comprises a sleeve, an armrest frame is embedded and fixed at the lower part of the outer side of the sleeve, a conveying pipe is embedded and fixed at the center of the bottom of the sleeve, a sliding block is matched with the inner side of the conveying pipe in an up-and-down sliding manner, a straight rack is fixedly connected at the center of the top of the sliding block, and the straight rack is fixedly connected with the handle. A transmission gear is rotationally meshed with the left side of the spur rack, the transmission gear leftwards extends out of the sleeve, a gap is reserved between the transmission gear and the sleeve, the outer side of the transmission gear is covered with a protective shell sleeve in an isolated mode, a rotating shaft is fixedly connected to the center of the front side of the transmission gear, and a distance adjusting knob is fixedly connected to the outer side of the front end of the rotating shaft; a common linear pushing mode is replaced with a gear rotating meshing mode, more stable controllability is achieved, force control is more accurate, the bone grain extrusion rate is more stable, in addition, compared with a partial spiral pushing mode, the transmission effect is more direct, and the extrusion rate is higher.

A surgical tool (10) for scraping and collecting bone particles, comprising: —a handle (20) provided with a handgrip (21); —a collection chamber (25) having an end proximal to the handgrip (21) and a free distal end; —a stem (30) at least partially arranged inside the collection chamber (25); —a blade (40) fixed to a front end (32) of the stem (30), wherein the blade (40) protrudes radially from the stem (30) and is axially arranged outside the collection chamber (25) near the distal end thereof; and—a spacer (50) interposed between a rear face (42) of the blade (40) and the distal end of the collection chamber (25), wherein the spacer (50) defines a passage opening (500) for introducing the bone particles scraped by the blade (40) into the collection chamber (25), maintaining at a predetermined non-zero axial distance, for at least a circumferential tract thereof, the rear face (42) of the blade (40) from the distal end of the collection chamber (25).

The invention provides a device and a method for preparing nanoscale ultrafine powder, and relates to the technical field of calcium particle preparation, the device comprises a solid-gas mixing cylinder body, a positive voltage cylinder, a negative voltage cylinder and a positive and negative ion impact tube; the solid-gas mixing cylinder body is provided with a material inlet, and the material inlet is used for inputting animal skeleton coarse particles and oxygen to form solid-gas mixed gas flow; the solid-gas mixing cylinder body is respectively communicated with the positive voltage cylinder and the negative voltage cylinder; the positive voltage cylinder ionizes oxygen in the solid-gas mixed gas flow into a positive ion state, and the negative voltage cylinder ionizes oxygen in the solid-gas mixed gas flow into a negative ion state; a positive-voltage cylinder is communicated with a discharge port of a negative-voltage cylinder, a solid-gas mixed gas flow with a positive ion state and a solid-gas mixed gas flow with a negative ion state converge at the discharge port and enter a positive-negative ion impact pipe, and the solid-gas mixed gas flow with the positive ion state and the solid-gas mixed gas flow with the negative ion state generate strong impact in the positive-negative ion impact pipe; a large amount of energy released when the positive and negative ions are combined crushes the animal skeleton coarse particles into ultrafine powder.

This invention relates to a bone regeneration material, characterized in that the bone regeneration material comprises the following components: cross-linked sodium hyaluronate gel, non-cross-linked sodium hyaluronate gel, and bone particles. The bone regeneration material provided by this invention possesses excellent cohesiveness and viscoelasticity, good water absorption and blood clot stability, a large specific surface area, and a porous microstructure, providing a favorable environment for cell adhesion, migration, and proliferation, and exhibiting excellent bone formation and osteoinduction effects.

The utility model discloses an anti-admission bone grafting tool which comprises a bone grafting channel pipe, a bone grafting funnel and a bone grafting bar assembly, the bone grafting bar assembly comprises a bone grafting pushing section and a bone grafting connecting section, a plurality of exhaust channels are arranged on the periphery of the bone grafting pushing section and the periphery of the bone grafting connecting section, the two ends of each exhaust channel are open, and the two ends of each exhaust channel are open. One end of the exhaust channel extends to the outer end of the bone grafting pushing section, and the other end of the exhaust channel extends to the outer end of the bone grafting connecting section. The utility model has the beneficial effects that in the process that the bone grafting pushing section pushes bone grafting bone particles along the interior of the bone grafting channel pipe, the exhaust channel is arranged, so that compressed gas can be discharged outwards in an airflow manner, and the gas is prevented from being compressed and pushed into a bone grafting position in the bone grafting process; therefore, the bone grafting particles can be compactly pressed at the bone grafting position, complications caused by air inflation in the femoral head are avoided, and the bone grafting operation risk can be reduced.

A method of making infused bone particles employs the following steps: cutting or shaving whole bone into bone particles, washing the bone particles, demineralizing or decalcifying at least partially the whole bone or bone particles and infusing the bone particles with a supernatant of biologic material or a polyampholyte cryoprotectant or a combination of both to create infused bone particles. The step of infusing includes exposing the bone particles to a negative pressure or vacuum to draw the supernatant and / or the polyampholyte cryoprotectant into the bone particles, or alternatively, exposing the demineralized whole bone to a positive pressure to drive the supernatant and / or the polyampholyte cryoprotectant into the bone. The resultant method creates an infused bone grafting composition having bone particles taken from whole bone, demineralized or decalcified at least partially and infused with one or more of a supernatant of biologic material or a polyampholyte cryoprotectant or both.

The utility model discloses a bone grafting device for open lumbar intervertebral fusion, which comprises a bone grafting funnel and a push rod, and the push rod can be inserted into a catheter to push bone grains in the catheter into intervertebral space and compact the bone grains so as to complete bone grafting. The bone grafting funnel comprises a catheter and a funnel groove, the catheter is through in the length direction, and the cross section of the catheter is rectangular, so that an exposed intervertebral space bone grafting inlet in an operation is fully utilized; the funnel groove is fixed at one end of the conduit along the length direction, an outlet of the funnel groove is communicated with the conduit, and an inlet of the funnel groove is exposed; the funnel groove is in a long and narrow groove shape, so that the funnel groove can be conveniently held by a hand in the process of pushing the push rod, particularly knocking the push rod to assist in pushing the push rod, the stability of the funnel groove is improved, and the funnel groove is prevented from shifting; the funnel grooves are symmetrically formed in the two sides of the catheter, balanced counterforce can be provided, the push rod can stably push bone grains into the intervertebral space, and the beneficial effects that the operation is convenient, the efficiency is high, and the safety is high are achieved.