4044results about "Connective tissue peptides" patented technology

A recombinant humanized collagen and a preparation method therefor. The recombinant humanized type III collagen can be efficiently and stably expressed in three expression systems of yeast, Escherichia coli, and CHO cells, and has the biological activity superior to that of a commercially available collagen product. The recombinant humanized type III collagen has structural characteristics and functional activity similar to those of a natural collagen, and thus has wide application prospects in biomedical materials, tissue engineering products, cosmetics, foods, health care products or drugs, medical instruments, medical cosmetology and the like.

The invention belongs to the technical field of cosmetics, and discloses anti-aging recombinant collagen as well as a preparation method and application thereof. The amino acid sequence of the anti-aging recombinant collagen is as shown in SEQ ID NO.1, and the nucleotide sequence of the anti-aging recombinant collagen is as shown in SEQ ID NO.2. The recombinant collagen CaI1 is constructed and expressed by recombining the effective sequences of the I-type collagen, the III-type collagen and the XVII-type collagen, and the recombinant collagen CaI1 is free of cytotoxicity, free of hidden danger of endotoxin and good in safety. The skin care product added with the recombinant collagen CaI1 can promote skin collagen absorption, inhibit skin aging and improve skin elasticity, and has a good application prospect.

The invention belongs to the technical field of optimized coding genes, and discloses a recombinant human I-type collagen as well as a preparation method and application thereof, and the amino acid sequence of the recombinant human I-type collagen is shown as SEQ ID No.1. The recombinant I-type collagen provided by the invention is relatively high in expression quantity, has relatively good cell migration promoting, cell proliferation promoting and cell adhesion promoting properties, is high in safety, and can be widely applied to the fields of medicines, medical instruments, biological materials, tissue engineering, cosmetics and the like.

The invention relates to a recombinant human XVII type collagen as well as a preparation method and application of the recombinant human XVII type collagen. The recombinant human XVII type collagen is any one of COL17A 3a, COL17A 3b, COL17A 3c, COL17A 3d or COL17A 3e. According to the invention, the functional fragments of the XVII collagen are analyzed by using computer software, the minimum functional region of the XVII collagen is screened, and expression and extraction are carried out by using an escherichia coli recombinant expression system, so that the target functional fragments with higher yield can be obtained.

The invention discloses a preparation method of yak bone collagen peptide for increasing bone mineral density and the yak bone collagen peptide. The invention provides a preparation method of yak bone collagen peptide for increasing bone mineral density. The preparation method comprises the following steps: providing yak bone powder; mixing the yak bone meal with a phosphate buffer solution, adjusting the pH value to 5-5.5, mixing with compound bacteria, and carrying out demineralization treatment to obtain a demineralized product; mixing the demineralized product with a compound enzyme, and carrying out an enzymolysis reaction to obtain an enzymolysis product; heating, centrifugally filtering and freeze-drying the enzymolysis product to prepare yak bone collagen peptide; wherein the compound enzyme comprises trypsin, alkaline protease and flavourzyme, and the compound bacteria comprise lactobacillus plantarum and propionibacterium. Lactobacillus plantarum and propionibacterium in the compound bacteria are subjected to synergistic fermentation, and are combined with the enzymolysis reaction of the compound enzyme, so that collagen is decomposed into small molecule peptides more efficiently, the extraction rate of bone collagen peptide is increased, and the bone mineral density is increased.

The invention relates to the field of biological medicines, in particular to a facial anti-aging collagen peptide, a composition and an application of the facial anti-aging collagen peptide, and particularly relates to a 21-type recombinant humanized collagen peptide (ColpepA1 21) and an application of a composition of the 21-type recombinant humanized collagen peptide in anti-aging treatment and skin care. The recombinant collagen provided by the invention can effectively promote the repair process after skin injury, enhance the hydration state and barrier function of the skin, reduce fine lines and wrinkles, and improve the compactness and elasticity of the skin.

The invention provides a recombinant humanized III-type collagen with a triple helix structure and preparation and application thereof, and belongs to the technical field of protein engineering, the amino acid sequence of the recombinant humanized III-type collagen comprises n core units, n is an integer of 1 or greater than 1, and the amino acid sequence of each core unit is as shown in SEQ ID NO: 1; the invention also provides a construction method of the expression system and a preparation method of the recombinant humanized III-type collagen. The invention also provides practical industrial application according to the characteristics of the protein. The recombinant humanized III-type collagen provided by the invention is high in purity, good in stability and high in biological activity, and experiments show that the recombinant humanized III-type collagen has various biological activities of promoting cell proliferation and migration and the like.

The invention provides recombinant human III-type collagen which is of a triple-helix structure and assembled into collagenous fibers and application of the recombinant human III-type collagen, and belongs to the technical field of bioengineering. Collagen fragments capable of forming a triple helix structure are screened out through a bioinformatics algorithm, the fragments are connected in series, a key C-terminal structural domain and a specific amino acid sequence are combined, and formation of a stable triple helix advanced structure and assembly of collagenous fibers are effectively promoted. The recombinant human III type collagen is efficiently expressed through a eukaryotic expression system, and shows excellent stability, low immunogenicity and good biocompatibility. Experimental results show that the prepared recombinant human III-type collagen not only has a natural triple helix structure, but also can be further assembled into macromolecular collagen fibers, is suitable for multiple fields such as medical beauty, bioengineering and tissue repair, and has wide application prospects and market values.

The invention discloses a recombinant humanized XVII type collagen as well as an expression vector and a genetically engineered bacterium thereof. Four functional fragments with high activity are obtained through screening, one of the fragments is optimized and then combined according to a certain sequence to obtain recombinant humanized XVII type collagen JYC1703, or one of the fragments is optimized and then combined according to a certain sequence, and GPP sequences are fused at an amino terminal and a carboxyl terminal respectively to obtain recombinant humanized XVII type collagen JYC1701 and JYC1702, and the recombinant humanized XVII type collagen JYC1701 and JYC1702 are combined according to a certain sequence to obtain the recombinant humanized XVII type collagen JYC1701 and JYC1702. After codon optimization, amplifying and cloning into an expression vector, transforming into pichia pastoris competent cells after enzyme digestion linearization, and screening high-copy recombinants through G418 resistance gradient, so as to obtain pichia pastoris genetically engineered bacteria for expressing recombinant humanized XVII type collagen. The recombinant humanized XVII type collagen JYC1701, JYC1702 and JYC1703 disclosed by the invention show excellent functions of promoting cell adhesion, cell proliferation and cell migration, and are suitable for preparing products such as skin care products or medical instruments and the like.

The present disclosure relates to a recombinant collagen, peptide fragments thereof, and sequence variants thereof. A novel Pichia pastoris strain for producing the recombinant collagen, fragments thereof, and sequence variants thereof, and methods of using the recombinant collagen, fragments, and sequence variants thereof are also provided. The present disclosure fulfills a need for environmentally friendly and safe alternatives for skincare.

The invention provides a recombinant IV type collagen composition for repairing a basement membrane and relieving and fading red. The inventor finds that the recombinant IV-type collagen disclosed by the patent specification inhibits VE-cadherin protein phosphorylation through a mediated PKC alpha / PYK2 / Src signal channel, protects tight connection between endothelial cells, prevents blood component leakage and reduces vascular permeability, and the recombinant IV-type collagen has the advantages that the recombinant IV-type collagen can inhibit the VE-cadherin protein phosphorylation through the mediated PKC alpha / PYK2 / Src signal channel; the dissociation of the VE-cadherin compound caused by oxidative stress can be improved, and the steady state of an endothelial barrier is maintained; therefore, the composition has good effects of repairing a basement membrane barrier, relieving and fading red. The inventor also finds that the repairing nonapeptide (GAAGLPGPK) has the effects of repairing the basal membrane barrier, relieving and fading red for the first time, and the combination of the recombinant IV type collagen and the nonapeptide in a certain proportion shows synergistic interaction in the aspects of repairing the basal membrane barrier, relieving and fading red.

A ternary deep eutectic solvent (TDES) system for a biomolecule (for example, chitin) recovery from a biomass (for example, seafood waste), and the method of preparation of ternary deep eutectic solvent (TDES) system, and a process to differentiate / extract / recover biomolecules (such as chitin) from a biomass (such as seafood waste).

The invention discloses recombinant human XVII type collagen as well as a preparation method and application thereof. The recombinant human XVII type collagen is obtained by repeating an amino acid sequence from natural human XVII type collagen. The amino acid sequence of the recombinant human XVII type collagen is derived from natural human XVII type collagen, and the recombinant human XVII type collagen does not generate adverse immune response when being used for a human body. The recombinant human XVII type collagen can be secreted and expressed in a commercial yeast expression system, the expression quantity is greater than 10g / L, the preparation method is simple, and the high-yield recombinant human XVII type collagen can be obtained at low cost; in addition, the cell adhesion promoting activity of the recombinant human XVII type collagen is obviously superior to that of human collagen.

The invention provides humanized recombinant XVII collagen for promoting hair follicle stem cell repair and resisting aging as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The humanized recombinant XVII type collagen comprises polypeptide derived from the XVII type collagen or repeated protein formed by N times of repeated series connection of the polypeptide serving as a unit, n is an integer from 2 to 4; the polypeptide derived from the XVII type collagen comprises a polypeptide A and / or a polypeptide B; the amino acid sequence of the A polypeptide is as shown in SEQ ID NO: 1; and the amino acid sequence of the B polypeptide is as shown in SEQ ID NO: 2. The humanized recombinant XVII type collagen not only can be massively and stably recombined and expressed, but also has cell adhesion activity, cell proliferation promotion activity, cell migration promotion activity and excellent hair follicle repair and regeneration promotion activity, and has no sensitization side effect. The invention provides a new way for preparing collagen products with rich functions.

The invention relates to a recombinant A type humanized XVII type collagen as well as construction and application thereof, the protein is the humanized XVII type collagen which is composed of an intracellular region, a transmembrane region and an extracellular region, and the amino acid sequence is as shown in SEQ No. 1. The hair follicle repairing agent has the advantages of hair follicle repairing, hair regeneration, good biological activities of cell proliferation, cell adhesion and the like, and can be widely applied to the fields of medicines, medical instruments, biological materials, tissue engineering, cosmetics and the like. The gene sequence of the recombinant A type XVII collagen is 100% identical to the corresponding part of the gene sequence of the recombinant A type XVII collagen, so that the recombinant A type XVII collagen has good hydrophilicity and high activity, and has application potential in the fields of biomedical materials, beauty cosmetics, skin care products and the like.

The invention relates to the field of biomedical materials, in particular to a novel composite collagen material for wound repair, and particularly relates to a preparation method of the novel composite collagen material containing 21-type recombinant humanized collagen (ColpepA1 21) and application of the novel composite collagen material in wound repair. The ColpepA1 21 is used for remarkably promoting key biological reactions in the wound healing process, including accelerating cell migration, enhancing the skin hydration state and barrier function and improving the stability and elasticity of the skin structure, a novel composite collagen material is developed, wound healing and cell regeneration can be effectively promoted, and oxidative damage can be relieved.

The invention provides a high-stability recombinant XVII type collagen as well as a construction method and application thereof, and belongs to the technical field of bioengineering. According to the invention, an amino acid sequence of natural human (XVII) type collagen is selected as a basic unit and repeated for multiple times to obtain recombinant (XVII) type collagen; in the design process of the recombinant XVII type collagen, an unstable GXY triad is avoided, potential matrix metalloproteinase restriction enzyme cutting sites and glycosylation sites are abandoned, a KGD active sequence is reserved, and the probability of containing sites which are easy to degrade can be reduced; a sequence fragment of the recombinant XVII type collagen is derived from a full-length sequence of a natural collagen gene, has excellent solubility, biological activity and high stability, and can be kept stable in a high-temperature storage environment and under the condition of avoiding alkalinity; the product can be used as a raw material in the production process of medicines, medical equipment, biological materials or cosmetics, and has good practicability.

The invention provides a pharmaceutical composition for preventing and treating gingival atrophy and a preparation method and application thereof, and belongs to the technical field of biological medicine. The composition contains finless eel peptide, skipjack elastin peptide, quinoa rice extract, gleditsia sinensis rice extract, calendula extract, chamomile extract, chitosan, peach gum, hydroxyapatite and the like, and the finless eel peptide, the skipjack elastin peptide, the quinoa rice extract and the gleditsia sinensis rice extract are specific enzymatic hydrolysates. The monopterus albus peptide, the skipjack elastin peptide, the quinoa rice extract and the gleditsia sinensis rice extract jointly promote repair and regeneration of gingival tissues. The calendula extract, the chamomile extract and the chitosan have anti-inflammatory and antibacterial effects, so that gingival inflammation is relieved, and bacterial infection is prevented. And the chitosan and the calendula extract can accelerate the repair of damaged gingival tissues. The monopterus albus peptide, the skipjack elastin peptide and the hydroxyapatite are matched for use to jointly maintain the normal structure and function of gingiva. All the components have a synergistic effect, so that gingival atrophy can be effectively prevented and improved.

The invention discloses a recombinant XII type collagen as well as a preparation method and application thereof. The amino acid sequence of the recombinant XII type collagen is any one of (A1)-(A3); (A1) is an amino acid sequence as shown in SEQ ID NO: 1; (A2) is an amino acid sequence which is obtained by substitution, deletion or insertion of one or more amino acid residues of the amino acid sequence as shown in SEQ ID NO: 1 and has the same functional protein; and (A3) is an amino acid sequence of a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of the sequence (A1) or (A2). The recombinant XII type collagen provided by the invention has good antioxidant activity, and has a repairing effect on the problems of skin laxity, wrinkle increase and the like caused by degradation of type I collagen fibers of aged skin.

The invention provides recombinant VII type collagen as well as a preparation method and application thereof, and relates to the technical field of bioengineering. According to the invention, the human-derived VII type collagen is designed and screened, so that a new functional repetitive unit with biological activity of the VII type humanized collagen is successfully obtained, and a new direction is provided for research of the VII type collagen. In the application, three new high-yield recombinant VII type humanized collagens are successfully obtained by repeating the functional repeating units. The recombinant VII type humanized collagen has the effect of improving cell viability, and the protein is proved to be high in safety. The protein also can promote cell adhesion, so that the protein has an excellent effect of treating skin injury. The protein can also improve the cell migration rate and has excellent transdermal absorption performance. In addition, the protein also has the effect of promoting collagen expression, and provides a new functional component for anti-aging related products.

The invention discloses a preparation method of cod skin collagen peptide with high ACE (angiotensin converting enzyme) inhibitory activity, which comprises the following steps: firstly, scaling cod skin, cleaning, cutting into small pieces, pretreating with NaOH and n-butyl alcohol to remove impure protein and certain lipid substances, preparing cod skin gelatin by a hot water extraction method, and then adding the cod skin gelatin into the cod skin collagen peptide to obtain cod skin collagen peptide; the cod skin collagen peptide with high ACE inhibitory activity is prepared by using cod skin collagen as a raw material, performing enzymolysis on the cod skin collagen peptide sequentially by using a double-enzyme distribution enzymolysis method, performing enzyme deactivation and centrifugal treatment after enzymolysis, and performing membrane separation on enzymolysis supernate. The whole enzymolysis process is more thorough by adopting a double-enzyme distribution enzymolysis method, more small molecule peptides can be obtained, cod skin collagen peptide with high ACE inhibitory activity can be further enriched by combining a membrane separation method, the utilization degree of cod skin resources is effectively improved, and the cod skin collagen peptide has a relatively wide application prospect.

The invention relates to recombinant III-type humanized collagen and application of a composition containing the same to eyes. The invention provides an application of recombinant III type humanized collagen in preparation of a product for preventing and / or treating eye inflammation, a composition containing the recombinant III type humanized collagen, and an application of the composition in preparation of a product for preventing and / or treating eye inflammation and / or eye dryness. The recombinant III-type humanized collagen and the composition containing the recombinant III-type humanized collagen can obviously promote updating and repairing of corneal epithelial cells, and meanwhile, can inhibit release of inflammatory mediators, enhance eye tissue barriers, enhance tear film stability and relieve eye inflammation and eye dryness.

The invention belongs to the technical field of bioengineering, and particularly relates to recombinant III-type humanized collagen as well as a coding gene and application thereof. According to the invention, a novel III-type humanized collagen sequence is designed by adopting an advanced AI technology and molecular dynamics simulation, so that the recombinant III-type humanized collagen product is ensured to have high stability and degradation resistance. According to the invention, the screened new III-type humanized collagen sequence is transferred to the pichia pastoris strain for recombinant expression, and the prepared recombinant III-type humanized collagen has the advantages of strong stability, high expression quantity and good hydrophilicity, and meanwhile, the recombinant III-type humanized collagen also has obvious effects of promoting cell proliferation and cell adhesion.

The invention provides a high-stability recombinant humanized elastin, a genetically engineered bacterium for expressing the protein and application of the high-stability recombinant humanized elastin, and belongs to the technical field of binding tissue peptides. The nucleotide sequence of the recombinant humanized elastin is shown as SEQ ID NO.1; and the amino acid sequence is as shown in SEQ ID NO.4. According to the invention, the nucleotide sequence of the humanized elastin is subjected to codon optimization and transferred into escherichia coli to construct the genetically engineered bacterium for expressing the recombinant humanized elastin, so that the expression quantity of the recombinant humanized elastin can be increased, the stability of the recombinant humanized elastin can be improved, and the recombinant humanized elastin can be used for preparing the recombinant humanized elastin. The migration rate of the recombinant humanized elastin to the HSF cell is improved, and the expression quantity of the h-ELN gene, the h-COL1A1 gene and the h-COL3A1 gene of the HSF cell is improved.

The invention discloses a method for preparing recombinant III-type human collagen. The method comprises the following steps: constructing genetically engineered bacteria for expressing proline hydroxylase and full-length recombinant III-type human collagen; and culturing the genetically engineered bacteria to obtain the recombinant III-type human collagen, the proline hydroxylase is prepared from P4Ha2, P4Hb and vP4H; the ratio of the copy number of the P4Ha2 to the copy number of the P4Hb to the copy number of the vP4H to the copy number of the full-length recombinant III type human collagen is (1.80 to 2.20) to (1.80 to 2.20) to (0.80 to 1.60) to 1. The method can be used for large-scale production, the purity is high, and the production cost can be reduced; the prepared recombinant III-type human collagen is completely consistent with the full-length sequence of natural III-type human collagen, the hydroxyproline content of the recombinant III-type human collagen is basically consistent with that of the natural III-type human collagen, and the recombinant III-type human collagen has a triple-helix structure, has the biological activity of the natural III-type human collagen, and is free of immunogenicity and virus hidden danger.

The invention belongs to the technical field of gene engineering and enzyme engineering, and particularly discloses an acid protease mutant and application thereof. According to the invention, genome DNA from Aspergillus niger is taken as a basis, a parent acid protease gene sequence is obtained through PCR amplification, random mutation is carried out through error-prone PCR, and an acid protease mutant with improved enzyme activity and a recombinant strain thereof are obtained through screening. After modification, under the optimal condition, the enzyme activity of the acid protease mutant is 251.64 U / mL, the enzyme activity is improved by 23.43% compared with that of parent acid protease (203.64 U / mL), meanwhile, the acid protease mutant is not prone to inactivation in the reaction process, has higher stability, can tolerate strong acid and high salt environments, shows a higher conversion rate in enzymolysis of collagen, and can be used for preparing the acid protease mutant. The leather softening agent has a good application prospect in leather softening.

The invention belongs to the technical field of protein engineering and genetic engineering, and particularly relates to recombinant IV-type humanized collagen as well as a preparation method and application thereof. A section of brand new recombinant collagen is designed and obtained through methods such as screening, replacement and splicing, the nucleotide sequence of the recombinant collagen is optimized through pichia pastoris codon preference, then a corresponding genetic engineering yeast strain is constructed and obtained, and the recombinant collagen is successfully prepared through fermentation. Experiments prove that the recombinant IV-type humanized collagen prepared by the invention is large in molecular weight and high in yield, and compared with commercially available recombinant collagen, the recombinant IV-type humanized collagen has better free radical scavenging performance and cell proliferation and migration promoting performance, and has good antioxidant and skin barrier repair functions, so that the recombinant IV-type humanized collagen has good practical application value.

The invention belongs to the technical field of genetic engineering, and particularly relates to recombinant collagen based on engineering bacterium expression and a preparation method thereof, and the recombinant collagen is formed by connecting repetitive units derived from human III type collagen in series for three times; the invention also discloses a base sequence of the coding gene of the recombinant collagen, efficient expression and accurate folding of target protein are realized through genetic modification of engineering bacteria, a light-operated dynamic folding process and an orthogonal purification strategy, and cascade purification of a His6-SUMO tag and an intein-CBD module is utilized to obtain the recombinant collagen. The product purity and the native conformation retention rate are remarkably improved, and the technical problems that hydroxyproline depends on exogenous addition, the folding controllability is poor and the purification efficiency is low in a traditional process are solved.

The invention belongs to the technical field of active substance extraction, and particularly relates to low-fat, low-cost, high-purity and high-quality elastin peptide and a preparation method thereof. The alkali liquor and the sodium carbonate solution are mixed and matched for use, the overall concentration is low, the degreasing agent is more environmentally friendly, and the degreasing effect is better by using the microwave heating technology. The protease is immobilized by using the microspheres, and the enzyme preparation can be fully recycled and repeatedly used, so that the production cost is greatly reduced. The elastin enzymatic hydrolysate is separated, purified and refined by using an ion exchange resin tandem chromatography technology, other impurities are efficiently removed, and the prepared elastin peptide is relatively high in purity. The high-voltage pulsed electric field technology (PEF) is used for sterilization, the sterilization effect is better, the influence of a traditional heat sterilization mode on the color, luster and taste of the product is avoided, and the quality of elastin peptide is better maintained.

The invention belongs to the technical field of protein engineering and biology, and discloses a recombinant III-type humanized collagen, a coding gene thereof, a protein preparation, a preparation method of the protein preparation, application of the protein preparation and a skin care product. The amino acid sequence of the recombinant III type humanized collagen is as shown in SEQ ID NO. 1. According to the recombinant III type humanized collagen, the transdermal absorption efficiency is greatly improved, and meanwhile, the proliferation and migration capabilities of keratinocytes are also remarkably improved. The collagen is widely applied to the fields of medical cosmetic plastic surgery, cosmetics and the like, and has a good application prospect.