970 results about "Hydrolysate" patented technology

The invention belongs to the technical field of health-care products, and particularly relates to a fermented composition beneficial to enhancing immunity and a preparation method thereof. The fermentation composition for improving immunity is prepared by taking yeast beta-glucan, an elderberry extract, glucoraphanin, sea cucumber peptide, inulin and a traditional Chinese medicine zymolyte as fermentation raw materials, firstly inoculating bacillus coagulans and staphylococcus xylosus for aerobic fermentation, and then inoculating bifidobacterium longum for anaerobic fermentation, the aerobic fermentation stage and the anaerobic fermentation stage have a synergistic effect, so that the fermentation speed can be increased, the fermentation time can be shortened, and richer nutrient substances can be provided, and therefore, the composition has an excellent effect of enhancing the immunity; the fermentation composition is applied to preparation of capsules helpful for enhancing immunity, is helpful for enhancing human immunity, and can solve the problem that active substances in existing health-care products are difficult to effectively absorb by human bodies, so that the effect of enhancing immunity is influenced.

The invention belongs to the field of microbial electrochemistry and solid organic waste recycling, and discloses a microbial electrosynthesis enhanced solid wastewater hydrolysis and anaerobic digestion biogas high-value utilization system coupling microbial electrosynthesis, organic solid waste electrochemical-biological synergistic hydrolysis oxidation and anaerobic digestion. The system comprises a cathode chamber and an anode chamber which are separated by an ion exchange membrane, an electrochemical driving device and an anaerobic digestion unit communicated with the anode chamber. And the cathode chamber is provided with a cathode electrode and an anaerobic acetic acid production microbial system and is communicated with biogas generated by anaerobic digestion, so that carbon dioxide in the biogas is reduced at the cathode to generate acetic acid and methane-enriched gas is output. The anode chamber is provided with an anode electrode, organic solid waste and a hydrolytic microorganism system are added, electrochemical-biological synergistic hydrolytic oxidation of the organic solid waste and hydrolysates of the organic solid waste is used as an anode reaction, oxygen evolution is inhibited, and oxygen transmembrane migration is reduced; and the hydrolysate in the anode chamber flows into the anaerobic digestion unit to produce biogas, so that the integrated operation of solid wastewater hydrolysis and biogas upgrading is realized.

The invention belongs to the technical field of medicine and health care, and particularly relates to a preparation method of an American ginseng root water extract rich in rare ginsenoside and application of the American ginseng root water extract in improving the immune and anti-tumor direction. The preparation method comprises the following steps: step 1, carrying out superfine grinding on American ginseng, and sieving; step 2, adding the American ginseng powder into water, and then adding compound enzyme for enzymolysis to obtain enzymatic hydrolysate; s3, after the enzymolysis is finished, heating the enzymatic hydrolysate, extracting twice, and mixing after the extraction is finished; and S4, inoculating compound lactic acid bacteria into the mixed solution, fermenting to obtain a fermentation solution, blending, and sterilizing to obtain the American ginseng afterbiogen beverage. According to the invention, a new physical-biological combined process is adopted, and crushing, enzymolysis, extraction, fermentation and blending are carried out, so that the American ginseng metagen beverage rich in rare ginsenoside is obtained, and the rare ginsenoside has a remarkable anti-tumor effect and higher activity.

The invention discloses a method for preparing fermentable sugar from recyclable low-concentration p-toluenesulfonic acid pretreated lignocellulose, which comprises the following steps: firstly, pretreating lignocellulose by using a low-concentration p-toluenesulfonic acid aqueous solution, and carrying out solid-liquid separation to obtain hemicellulose hydrolysate and a pretreated solid component; then saccharifying and pretreating the solid component by using cellulase to obtain hydrolysate rich in glucose, adsorbing the hemicellulose hydrolysate by using hypercrosslinked adsorption resin, carrying out solid-liquid separation to obtain hydrolysate rich in xylose, and carrying out desorption treatment on the resin by using industrial ethanol to obtain xylose; and performing rotary evaporation on the desorbed liquid to remove the industrial ethanol to obtain p-toluenesulfonic acid. According to the method, hemicellulose is efficiently degraded to obtain derived sugar of hemicellulose, meanwhile, the saccharification efficiency of cellulase is improved, p-toluenesulfonic acid is recycled through the hypercrosslinked adsorption resin, and the method has the advantages of being high in fermentable sugar yield, recyclable in acid catalyst, low in cost, environmentally friendly and the like.

The invention discloses a hexapeptide with a hypoglycemic effect as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The amino acid sequence of the hexapeptide is Ile-Trp-Asp-Pro-His-Phe, and the amino acid sequence of the hexapeptide is as shown in the specification. The novel hexapeptide IWDPHF with prominent DPP-IV inhibition ability is successfully identified from mulberry leaf proteolysis products through liquid chromatography-mass spectrometry and a virtual screening method, the hexapeptide IWDPHF shows the blood glucose reducing effect superior to that of part of known peptides in an in-vitro enzyme activity inhibition experiment and a zebra fish hyperglycemia model, and no obvious side effect exists; the compound has a good potential of being developed into a hypoglycemic functional product or a drug lead compound. The invention further provides the mulberry leaf peptide with the peptide fragment IWDPHF, and the mulberry leaf peptide can be applied to preparation of drugs or food for reducing blood sugar. The invention provides a new scheme and theoretical basis for treatment of diabetes, and has good market prospect and application potential.

The invention provides a whey protein deep hydrolysate with low sensitization and an allergy relieving effect as well as a preparation method and application of the whey protein deep hydrolysate. According to the method, the alkaline protease, the neutral protease and the glutamyltransferase are sequentially used for carrying out first enzymolysis, second enzymolysis and third enzymolysis on the whey protein raw material, and the whey protein hydrolysate which is low in molecular weight, good in taste, good in digestion and absorption performance, low in sensitization and capable of effectively relieving allergic reaction of the organism is obtained.

The invention discloses a screening method of hericium erinaceus functional polypeptide based on artificial intelligence assistance and polypeptide or a polypeptide composition. The method comprises the following steps: (1) constructing an AI auxiliary annotation model; (2) extracting hericium erinaceus crude protein; (3) preparing hericium erinaceus protein enzymatic hydrolysate through composite enzymatic hydrolysis; (4) performing polypeptide identification on the hericium erinaceus protein enzymatic hydrolysate to obtain a polypeptide sequence; and (5) carrying out AI auxiliary annotation and polypeptide screening by using a large language model based on a DeepSeek platform so as to obtain a polypeptide sequence with assumed biological activity. The polypeptide sequence and the polypeptide composition which have remarkable ACE inhibition and oxidation resistance dual functions are successfully obtained by utilizing the method disclosed by the invention. Compared with a traditional experience screening path, the method realizes a high-throughput active peptide development process which is targeted in structure, accurate in prediction and capable of verifying a closed loop.

The invention discloses a preparation method of fresh taxus chinensis stem and leaf protoplasts. The preparation method comprises a preparation process and a purification process. The preparation method of the taxus chinensis stem and leaf protoplast is simple and easy to operate, raw materials are easy to obtain and low in price, reagent components have good biocompatibility, and the taxus chinensis stem and leaf protoplast is free of harmful components, safe and environmentally friendly. The invention provides a simple and rapid taxus chinensis stem and leaf protoplast enzymolysis preparation method, which adopts vacuum filtration, accelerates the permeation of enzymatic hydrolysate and improves the enzymolysis efficiency, so that the protoplast can be rapidly obtained. Meanwhile, high-purity separation of the protoplast is carried out in combination with density gradient sedimentation of the iodixanol solution, so that the protoplast with uniform size and complete form is obtained, and convenience is provided for scientific researches such as subsequent conversion and single cell transcriptome sequencing.

The invention discloses a nutritional composition for promoting neurodevelopment and application, and provides a nutritional composition for promoting neurodevelopment, which comprises the following necessary components: a sialylated substance component comprising one or more of N-acetylneuraminic acid, sialylated oligosaccharide, sialylated protein and sialylated lipid; the whey protein component comprises alpha-lactalbumin; and a peptide component from casein, including a hydrolysate of casein; the nutritional composition comprises a sialylated substance component, a whey protein component and a casein-derived peptide component, and the mass ratio of the sialylated substance component, the whey protein component and the casein-derived peptide component in the nutritional composition is (0.5-10): (300-1): (100-0.5). The nutritional composition provided by the invention can promote brain development and cognitive functions, and can significantly promote neurodevelopment by scientifically matching the sialylated substance component, the whey protein component and the peptide component from casein.

The invention relates to the technical field of plant extraction, in particular to a multi-product preparation method based on an arabinoxylan-lignin compound. The method comprises the following steps: treating a lignocellulose raw material by adopting a weak base extracting solution and a strong base extracting solution, and separating an obtained strong base extracting and dealkalizing suspension into an arabinoxylan-lignin compound dispersion liquid and arabinoxylan; performing enzymolysis on the dispersion liquid, and filtering enzymatic hydrolysate to obtain a precipitate which is lignin and a filtrate which is xylooligosaccharide liquid; and separating and purifying the xylooligosaccharide liquid to obtain xylooligosaccharide, xylose and arabinose. According to the method, on the basis of a weak base-strong base two-step extraction process, obtained arabinoxylan-lignin compound dispersion liquid is subjected to enzymolysis, so that lignin, xylooligosaccharide, xylose and arabinose can be continuously prepared, and the reutilization of the arabinoxylan-lignin compound is realized.

The invention discloses extraction of polydeoxyribonucleotide as well as preparation and application of a PDRN-Ce (Polydeoxyribonucleotide-Ce) microsphere cluster. The extraction and purification method comprises the following steps: mixing in-vitro fish testis tissues with water, carrying out crushing treatment and homogenization treatment on an obtained premix, carrying out proteolysis and solid-liquid separation, and collecting supernate to obtain enzymatic hydrolysate; wherein protease used for proteolysis comprises neutral protease; carrying out alcohol precipitation on the enzymatic hydrolysate by adopting an alcohol solvent, and collecting a precipitate; and dissolving the precipitate in water, desalting and drying. According to the extraction method, the purity and the yield of the poly-deoxyribonucleotide are remarkably improved, the preparation method is low in cost and convenient to operate, the prepared poly-deoxyribonucleotide is stable in property, small in molecular weight and easy to absorb by skin, in order to further improve the bioavailability of the extracted poly-deoxyribonucleotide, the PDRN-Ce microsphere cluster is prepared, and the PDRN-Ce microsphere cluster can be used for preparing the poly-deoxyribonucleotide. Good application prospects are realized.

The invention relates to a method for extracting and purifying lycium barbarum polyphenol by combining bacteria and enzymes. The method comprises the following steps: pretreating lycium barbarum fruits to obtain lycium barbarum powder; extracting the wolfberry powder by using a first solvent to obtain a first crude extract; fermenting the first crude extract with lactobacillus plantarum to obtain a second crude extract; performing enzymolysis on the second crude extract by using pectinase, hemicellulase and beta-glucosidase in sequence to obtain an enzymolysis extracting solution; adsorbing and purifying the enzymolysis extracting solution by using first ion exchange resin, washing and eluting to obtain a first eluent; adsorbing and purifying the first eluent by using second ion exchange resin, washing and eluting to obtain a second eluent; and concentrating and drying the second eluent to obtain wolfberry polyphenol powder. According to the method disclosed by the invention, the fermentation of lactobacillus plantarum is combined with the synergistic effect of pectinase, hemicellulase and beta-glucosidase, polysaccharide in a crude extract is decomposed, polyphenol is released and combined, the extraction rate and the purity of the polyphenol are improved, and meanwhile, a continuous purification process of macroporous resins AB-8 and D-101 is combined, so that the product quality is further improved.

The invention relates to the technical field of soil amendments, in particular to a soil amendment containing earthworm enzymatic hydrolysate and a preparation method of the soil amendment. The soil conditioner containing the earthworm enzymatic hydrolysate comprises the following components: earthworm enzymatic hydrolysate microcapsules and adjuvant particles in a mass ratio of (2-4): 1, the earthworm enzymatic hydrolysate is prepared by performing enzymolysis on fresh earthworm homogenate by compound enzyme, and the compound enzyme comprises cellulase and protease; the wall material of the earthworm enzymatic hydrolysate microcapsule comprises at least one of chitosan, calcium alginate, gelatin and Arabic gum; the auxiliary agent comprises humic acid and inorganic natural minerals. According to the soil conditioner containing the earthworm enzymatic hydrolysate, the earthworm enzymatic hydrolysate is wrapped by the microcapsules, the retention rate of active ingredients in the earthworm enzymatic hydrolysate can be remarkably increased, and the soil condition can be further improved by matching with humic acid and inorganic natural minerals for use.

This invention discloses a recombinant collagen liposome, its preparation method, and its application. The recombinant collagen liposome comprises 1-10 parts recombinant type XVII collagen, 2-15 parts soybean lecithin, 0.3-2 parts tocopherol, 0.5-7 parts antioxidant, 20-54 parts glycerol, and Dendrobium officinale enzymatic hydrolysate to a total weight of 100 parts. This invention improves the stability and transdermal absorption of recombinant type XVII collagen by encapsulating it in liposomes. The prepared recombinant collagen liposomes not only have a high encapsulation efficiency but also retain excellent barrier repair and anti-wrinkle effects after 8 weeks of storage, making them suitable for use in cosmetics.

The invention belongs to the technical field of composite processing aids, and particularly relates to a processing aid for a tensile CPVC (chlorinated polyvinyl chloride) board and a preparation process thereof, and the preparation process comprises the following steps: dispersing glass fibers in a KH-550 hydrolysate for stirring reaction, dispersing in a bisphenol A epoxy resin solution for stirring reaction after washing and drying, dispersing in an ODI (Olefin Diisocyanate) solution for stirring reaction after washing and drying, and drying to obtain the tensile CPVC board. After washing, dispersing in a GMA solution for stirring reaction, and washing and drying to obtain modified glass fibers; and dispersing the modified glass fiber in the dispersion liquid, adding an initiator and BA, heating, stirring for reaction, adding an initiator and MMA, stirring for reaction, washing and drying to obtain the processing aid. When a CPVC plate prepared by blending the processing aid and CPVC is used in a high-temperature and high-humidity environment, high tensile strength and impact strength can be kept for a long time.

The invention discloses a pretreatment method for increasing the yield of anaerobic fermentation methane of a lignocellulose material, and belongs to the technical field of high-efficiency anaerobic fermentation methane production of organic solid waste. The method comprises the following steps: (1) crushing straws; (2) mixing the straw powder and deionized water according to a mass ratio of 1: (19-21), stirring at a rotating speed of 120-180r / min, treating for 22-26h, fully and uniformly mixing, adjusting the pH value of the system to 3.4-3.7, adding 0.08-0.12 wt% of cellulase according to the mass of the straw, and carrying out enzymolysis at 49-51 DEG C for 2-4d to obtain an enzymatic hydrolysate; (3) yeast pre-fermentation: inoculating 4-6% v / v activated angel high-activity dry yeast into the enzymatic hydrolysate in the step (2) for anaerobic pre-fermentation, wherein the pre-fermentation time is 16-20 hours; and (4) anaerobic fermentation for methane production: adding 20-30% v / v of introduction sludge into the fermentation system in the step (3), and carrying out anaerobic fermentation for methane production, wherein the fermentation temperature is 36-38 DEG C. Compared with the prior art, the method has the advantages of being high in adaptability, short in treatment period, good in economic benefit, free of environmental pollution and the like, and has certain guidance and inspiration effects on engineering of producing methane from straw.

The invention belongs to the field of chemical engineering, and relates to a process device and method for continuously synthesizing glycidyl (3-chloro-1, 2-propylene glycol and sodium hydroxide (hereinafter referred to as NaOH) as raw materials). The device comprises a raw material feeding pump, a heat exchanger, a micro-reactor, a delay tube reactor 1, a neutralization micro-reactor and a delay tube reactor 2 which are sequentially in fluid communication through pipelines, the heat exchangers are divided into two groups, one group is used for heat exchange of 3-chloro-1, 2-propylene glycol solution, and the other group is used for heat exchange of alkaline solution. According to the method, a 3-chloro-1, 2-propylene glycol solution and a sodium hydroxide solution (NaOH) are adopted as raw materials, glycidol is continuously prepared through a microchannel reactor, on the basis, an acid-base neutralization reaction can be carried out in situ according to needs to remove redundant alkali, redundant heat generated by the acid-base neutralization reaction is absorbed through a low-temperature material, and energy consumption is reduced; the product is analyzed by gas chromatography, and the generated glycidol does not have hydrolysates or auto-polymerization products, so that the product selectivity is higher.

The invention relates to an instant composition with a long-acting oral health-care function for dogs and cats as well as a preparation method and application of the instant composition, and belongs to the technical field of pet food and pet oral care. The composition comprises 20%-30% of whey protein powder, 10%-20% of hydrolyzed gelatin, 1%-3% of low-molecular-weight chitin saccharide gum, 1%-2% of modified cassava starch, 2%-5% of sodium alginate, 1%-3% of fructo-oligosaccharide, 0.03%-0.08% of lysozyme, 0.03%-0.08% of tea polyphenol, 0.05%-0.15% of zinc gluconate, 0.05%-0.1% of persimmon tannin, 3%-5% of tuna hydrolysate, 4%-6% of whole goat milk powder, 2%-4% of skim yoghurt powder and the balance water. The added active ingredients have long-acting slow-release and continuous effects in the formula, oral pathogenic bacteria can be effectively inhibited, dental plaque and dental calculus formation can be reduced, halitosis can be lastingly relieved, and the toothpaste has good market competitiveness.

The invention discloses an enzymolysis preparation method of high-immunocompetence bovine coloctrum peptide, active peptide and application thereof, and belongs to the field of biotechnology and functional foods.The enzymolysis preparation method is characterized by comprising the steps that bovine coloctrum is degreased, trehalose is added into obtained supernate, first stirring is conducted, and first mixed liquid is obtained; wherein the supernate comprises whey protein and Ig; adding medium protease into the first mixed solution for hydrolysis to obtain enzymatic hydrolysate; wherein the enzymatic hydrolysate comprises a mixture of bovine colostrum hydrolyzed peptides, the Ig activity retention rate is larger than or equal to 90% through the targeted protection and directional enzymolysis technology, the beta-lactoglobulin peptide with the synergistic immune enhancement effect is obtained, the Ig and small molecule peptides in the product have the synergistic effect, the body immune regulation capacity and the intestinal immune function are remarkably improved, the immunity of the human body is improved, and the immunity of the human body is improved. The method can be widely applied to the fields of infant formulas, postoperative nutrition, functional drinks and the like.

The invention provides low-molecular-weight tea polysaccharide and a preparation method and application thereof, and belongs to the technical field of tea polysaccharide extraction.The preparation method of the low-molecular-weight tea polysaccharide comprises the following steps that 1, tea powder, compound enzyme and water are mixed to obtain an enzymolysis system, and enzymolysis is conducted to obtain enzymatic hydrolysate; 2) performing ultrasonic-microwave synergistic extraction on the enzymatic hydrolysate, and then boiling and extracting to obtain an extracting solution; (3) inoculating compound microorganisms into the extracting solution, and fermenting to obtain fermentation liquor; 4) performing ultrafiltration and concentration on the fermentation liquor to obtain a tea polysaccharide solution; 5, the tea polysaccharide solution is subjected to affinity membrane chromatography and elution, eluent is subjected to reverse filtration through a nanofiltration membrane, trapped fluid is collected, and the low-molecular-weight tea polysaccharide is obtained.The molecular weight of the low-molecular-weight tea polysaccharide ranges from 2 KDa to 5 KDa, and the low-molecular-weight tea polysaccharide can be more easily absorbed by the human body and has the effect of rapidly and remarkably adjusting blood sugar.

The invention discloses an anti-muscle attenuation composition and application thereof, and the composition provided by the invention comprises an anti-muscle attenuation peptide, WPC, MLCT and CaHMB; the muscle attenuation resisting peptide is zymolyte obtained after milk protein is subjected to protease enzymolysis, the zymolyte comprises polypeptide with the absolute net charge density ridge-shaped region distribution of a peptide chain formed by a head peptide fragment, a middle peptide fragment and a tail peptide fragment, the head peptide fragment is j1, the middle peptide fragment is j2, and the tail peptide fragment is j3; the charge density of the j1 and the charge density of the j3 are both larger than the charge density of the j2, or the charge density of the j1 and the charge density of the j3 are both smaller than the charge density of the j2. Through mutual cooperation of the muscle attenuation resisting peptide, the WPC, the MLCT and the CaHMB, the effect of obviously improving the muscle attenuation resisting effect is achieved.

The invention provides a preparation method and application of anti-aging and cell-repairing pancreas biological zymolyte, and relates to the technical field of enzyme viscera extracts. The preparation method comprises the following steps: pretreating pancreas, adding water, pulping, boiling, cooling to room temperature, and adjusting the pH value; adding alkaline protease, heating for enzymolysis, dynamically adjusting the temperature and pH during enzymolysis, and inactivating after enzymolysis is finished to obtain enzymatic hydrolysate; adding a reducing agent into the enzymatic hydrolysate, then adding staphylococcus xylosus liquid, carrying out two-stage fermentation, taking supernate after fermentation is finished, and carrying out ultrafiltration concentration; the staphylococcus xylosus bacterial liquid is produced from staphylococcus xylosus YB-12, the staphylococcus xylosus YB-12 is preserved in the China General Microbiological Culture Collection Center on January 3, 2023, and the preservation number of the staphylococcus xylosus YB-12 is CGMCC No. 26373. The finished product prepared by the method is high in stability; meanwhile, the zymolyte can be applied to cell repair or anti-aging.

The invention discloses an Ectoin and hydrolyzed collagen silanol composition as well as a preparation method and application thereof. The preparation method comprises the following steps: preparing a hydrolyzed collagen silanol derivative: hydrolyzing a silane coupling agent in an acidic organic solvent aqueous solution to obtain silanol hydrolysate; the preparation method comprises the following steps: dissolving hydrolyzed collagen in deionized water, adjusting the pH value to 8-10, stirring, adding silanol hydrolysate for reaction, cooling to room temperature, and freeze-drying to obtain hydrolyzed collagen silanol derivative powder; the preparation method comprises the following steps: preparing an Ectoine and hydrolyzed collagen silanol composition: dissolving hydrolyzed collagen silanol derivative powder and Ectoine in pure water together, and carrying out ultrasonic treatment to obtain the Ectoine and hydrolyzed collagen silanol composition, the prepared composition can inhibit skin heat injury, inhibit skin inflammatory response, promote skin collagen regeneration and repair skin barrier, and can be applied as a raw material of skin care products or medical beauty products.

A high-purity tin composition that can maintain high purity for a long time, in which decomposition of a triaminotin compound is suppressed and high purity of the triaminotin compound is maintained, is provided. The tin composition contains 95 mol % or more of a triaminotin compound having formula (1) in terms of tin atoms, and 0.001 to 0.5 mol % of a tetraaminotin compound having formula (2) in terms of tin atoms:R is a hydrocarbon group having 1 to 30 carbon atoms which is optionally substituted with a halogen, an oxygen atom, or a nitrogen atom, and R's are each a hydrocarbon group having 1 to 10 carbon atoms and may be identical or different from each other; two R's on the same nitrogen atom may be bonded to each other to form a 3- to 7-membered ring containing nitrogen.

The invention relates to the technical field of separation and extraction, and provides an extraction method and application of chlorogenic acid in honeysuckle. The invention relates to a method for extracting chlorogenic acid from honeysuckle, which comprises the following steps: mixing honeysuckle powder with an ethanol solution, and carrying out ultrasonic enzymolysis; carrying out suction filtration to obtain a supernatant A containing chlorogenic acid and medicine residues I, and adding an ethanol solution into the medicine residues I to carry out microwave extraction; performing suction filtration to obtain a filtrate B and medicine residues II, mixing the medicine residues II with a citric acid-sodium citrate buffer solution, adding a Fe3O4-coated GO-coated MOFs nano-catalyst, performing hydrothermal reaction, performing suction filtration to obtain hydrolysate, and combining the hydrolysate with the supernate A and the filtrate B to obtain a total extraction solution; concentrating the total extracting solution under reduced pressure, and recovering ethanol until the volume of the concentrated solution is about 1 / 6-1 / 8 of the original volume; and concentrating under reduced pressure to thick paste, and drying to obtain the chlorogenic acid. The extraction efficiency and yield of chlorogenic acid are improved, and the utilization rate of raw materials and the product purity are improved.

The invention discloses a method for recycling PA66 / PET (Polyamide 66 / Polyethylene Terephthalate) mixed waste. The recycling and regenerating method comprises the following steps: under the action of a catalyst, carrying out hydrolysis reaction on the PA66 / PET mixed waste; wherein the catalyst is adipic acid, and the use amount of the catalyst is 10-30 wt% of the amount of the mixed waste according to the mass percentage. An adipic acid autocatalysis system is adopted, efficient hydrolysis of PA66 and PET is achieved at the same time under the single reaction condition, the defect that step-by-step treatment is needed in a traditional process is overcome, the hydrolysis rate of PET is larger than or equal to 85%, the hydrolysis rate of PA66 is 50-70%, hydrolysis products can be further polymerized into polyesteramide with excellent mechanical performance and thermal stability, and the preparation method is suitable for industrial production. And the spanning from degradation recovery to upgrading regeneration is realized.

The present disclosure provides polypeptides having a cholesterol-lowering function in a soybean protein hydrolysate, a preparation method thereof, and uses thereof. The polypeptides are derived from peptide fragments having a molecular docking “binding energy” value of less than −1.2 kcal with cholesterol esterase. The peptide fragments of the polypeptides that are subjected to molecular docking with cholesterol esterase are derived from 23 polypeptides having a Peptide Ranker bioactivity score of greater than 0.9. The polypeptides possess a significant blood-lipid-lowering function.

The invention relates to the technical field of nanofiber membrane materials, in particular to a heat-insulation and warm-keeping nanofiber membrane material and a preparation method thereof.A mixed solution of tetraethoxysilane hydrolysate and a polyvinyl alcohol aqueous solution serves as a precursor of electrostatic spinning, modified titanium dioxide hollow microspheres are added in the preparation process, and the nanofiber membrane material is prepared; a near-vacuum environment is formed in the titanium dioxide hollow microspheres, heat is conducted by inhibiting collision of gas molecules, so that radiation heat transfer is blocked, the prepared nanofiber membrane material has a good heat preservation and heat insulation effect, a silane coupling agent forms hybrid bonds on the surfaces of the microspheres and is combined with polyvinyl alcohol molecular chains through chemical bonds, and the heat preservation and heat insulation effects are improved. The interface adhesion force is obviously improved, so that the hollow microspheres are prevented from collapsing during mechanical friction or bending; secondly, different types of quaternary ammonium salt antibacterial agents are compounded, the synergistic sterilization effect can be achieved, four quaternary ammonium salts are synergistic through different action targets, and the problem that a single antibacterial agent is prone to generating drug resistance can be solved.

The invention relates to an osmanthus protoplast preparation method and an instantaneous conversion method. The preparation method of the sweet-scented osmanthus protoplast comprises the following steps: collecting different tissues of fresh sweet-scented osmanthus, performing surface disinfection and sterilization, cutting into thin strips or slices, adding enzymatic hydrolysate for enzymolysis, filtering, centrifuging, washing with a W5 solution, and re-suspending. The instantaneous transformation method of the osmanthus protoplast comprises the following steps: adjusting the concentration of the osmanthus protoplast by using an MMG solution, adding plasmids for mixing, adding a PEG solution for transformation incubation, adding a W5 solution for washing and centrifuging, and detecting the instantaneous transformation efficiency of the osmanthus protoplast. According to the method, a high-quality protoplast separation system of the leaves, the stems and the petals of the sweet-scented osmanthus is successfully established, efficient conversion of mesophyll and petal protoplast is realized for the first time, and a reliable technical platform is provided for molecular biology research of the sweet-scented osmanthus.

The invention discloses a high-yield and high-activity pear protoplast extraction method based on light shielding and enzymolysis optimization, which comprises the following steps: by taking pear tissue culture seedling leaves growing for 30-40 days or field pear tender leaves as an extraction material, carrying out enzymolysis treatment and protoplast purification by adopting enzymatic hydrolysate to obtain protoplast; before enzymolysis of the pear tissue culture seedlings, the whole pear tissue culture seedlings are placed in a dark environment and subjected to dark pretreatment at 25 + / -2 DEG C for 48-72 hours. According to the method, the yield of the field pear leaf and tissue culture seedling pear leaf protoplast can be increased by more than or equal to 2 times (the highest protoplast yield is greater than or equal to 3.0 * 10 / g FW), the activity is increased to be greater than or equal to 95%, and the technical bottleneck of the existing patent is remarkably broken through. The method has the advantages of high yield, high activity, wide application range and the like, can provide a high-quality protoplast material for pear genetic regeneration, gene editing, single cell transcriptome analysis and metabonomics research, and has wide scientific research and industrial application potential.