1727 results about "Hydrolysate" patented technology

The invention discloses tetrapeptide KA4 with an anti-fatigue function as well as a preparation method and application of the tetrapeptide KA4, and belongs to the technical field of biology. The tetrapeptide KA4 is screened from pacific salmon protamine enzymatic hydrolysate, the amino acid sequence is KYPA, and the tetrapeptide KA4 has strong binding capacity with KEAP1, AMPK and LDH, can significantly reduce the serum lactic acid content of a fatigue model mouse and significantly increase the load swimming time of the fatigue model mouse, has an anti-fatigue function, and can be applied to development of functional food for relieving fatigue.

The preparation method comprises the following steps: S1, crushing cordate houttuynia rhizomes, adding the crushed cordate houttuynia rhizomes into a mixed acid aqueous solution, and carrying out immersion treatment to obtain a cordate houttuynia mixed solution; s2, adjusting the pH value of the herba houttuyniae mixed solution to 4-5, adding pectinase, carrying out primary enzymolysis and enzyme deactivation, adjusting the pH value to 6-7, adding compound enzyme, carrying out secondary enzymolysis and enzyme deactivation, so as to obtain an enzymatic hydrolysate; s3, menthol is added into the enzymatic hydrolysate, water extraction is conducted, and water extract and filter residues are obtained; s4, the filter residues are added into ethyl alcohol, then salicylic acid and diacetyl tartaric acid ester of mono (di) glycerides are added, alcohol extraction and filtering are conducted, and an alcohol extraction solution is obtained; s5, mixing the water extract with the alcohol extract, and performing reduced pressure distillation to obtain the herba houttuyniae extract. According to the method, the extraction rate of polysaccharide and flavone in houttuynia cordata can be effectively increased, and meanwhile, the prepared houttuynia cordata extract has better oxidation resistance and has a good application prospect.

The invention belongs to the technical field of health-care products, and particularly relates to a fermented composition beneficial to enhancing immunity and a preparation method thereof. The fermentation composition for improving immunity is prepared by taking yeast beta-glucan, an elderberry extract, glucoraphanin, sea cucumber peptide, inulin and a traditional Chinese medicine zymolyte as fermentation raw materials, firstly inoculating bacillus coagulans and staphylococcus xylosus for aerobic fermentation, and then inoculating bifidobacterium longum for anaerobic fermentation, the aerobic fermentation stage and the anaerobic fermentation stage have a synergistic effect, so that the fermentation speed can be increased, the fermentation time can be shortened, and richer nutrient substances can be provided, and therefore, the composition has an excellent effect of enhancing the immunity; the fermentation composition is applied to preparation of capsules helpful for enhancing immunity, is helpful for enhancing human immunity, and can solve the problem that active substances in existing health-care products are difficult to effectively absorb by human bodies, so that the effect of enhancing immunity is influenced.

The invention belongs to the field of microbial electrochemistry and solid organic waste recycling, and discloses a microbial electrosynthesis enhanced solid wastewater hydrolysis and anaerobic digestion biogas high-value utilization system coupling microbial electrosynthesis, organic solid waste electrochemical-biological synergistic hydrolysis oxidation and anaerobic digestion. The system comprises a cathode chamber and an anode chamber which are separated by an ion exchange membrane, an electrochemical driving device and an anaerobic digestion unit communicated with the anode chamber. And the cathode chamber is provided with a cathode electrode and an anaerobic acetic acid production microbial system and is communicated with biogas generated by anaerobic digestion, so that carbon dioxide in the biogas is reduced at the cathode to generate acetic acid and methane-enriched gas is output. The anode chamber is provided with an anode electrode, organic solid waste and a hydrolytic microorganism system are added, electrochemical-biological synergistic hydrolytic oxidation of the organic solid waste and hydrolysates of the organic solid waste is used as an anode reaction, oxygen evolution is inhibited, and oxygen transmembrane migration is reduced; and the hydrolysate in the anode chamber flows into the anaerobic digestion unit to produce biogas, so that the integrated operation of solid wastewater hydrolysis and biogas upgrading is realized.

The invention belongs to the technical field of medicine and health care, and particularly relates to a preparation method of an American ginseng root water extract rich in rare ginsenoside and application of the American ginseng root water extract in improving the immune and anti-tumor direction. The preparation method comprises the following steps: step 1, carrying out superfine grinding on American ginseng, and sieving; step 2, adding the American ginseng powder into water, and then adding compound enzyme for enzymolysis to obtain enzymatic hydrolysate; s3, after the enzymolysis is finished, heating the enzymatic hydrolysate, extracting twice, and mixing after the extraction is finished; and S4, inoculating compound lactic acid bacteria into the mixed solution, fermenting to obtain a fermentation solution, blending, and sterilizing to obtain the American ginseng afterbiogen beverage. According to the invention, a new physical-biological combined process is adopted, and crushing, enzymolysis, extraction, fermentation and blending are carried out, so that the American ginseng metagen beverage rich in rare ginsenoside is obtained, and the rare ginsenoside has a remarkable anti-tumor effect and higher activity.

The invention discloses camel milk polypeptide with xanthine oxidase inhibitory activity and application thereof.The camel milk polypeptide is prepared by taking fresh camel milk as a raw material, conducting low-temperature centrifugation to remove fat, conducting triple enzymolysis through papain, pepsin and trypsin to obtain enzymatic hydrolysate, enabling the enzymatic hydrolysate to pass through an ultrafiltration membrane (the molecular weight cutoff is 10 kDa) to obtain filtrate, and conducting freeze-drying to obtain the camel milk polypeptide. The compound has xanthine oxidase inhibitory activity. The invention also discloses a small molecule polypeptide with xanthine oxidase inhibitory activity. The filtered solution is screened and combined with polypeptides by adopting a xanthine oxidase affinity ultrafiltration method, the activity of the polypeptides is verified through molecular docking and in-vitro enzyme inhibition, four small-molecule polypeptides with xanthine oxidase inhibitory activity are obtained, the sequence of the small-molecule polypeptides is shown as any one of SEQ ID NO.1-4, and the small-molecule polypeptides can be used for preparing drugs with the effect of inhibiting the uric acid level.

The invention discloses a method for preparing fermentable sugar from recyclable low-concentration p-toluenesulfonic acid pretreated lignocellulose, which comprises the following steps: firstly, pretreating lignocellulose by using a low-concentration p-toluenesulfonic acid aqueous solution, and carrying out solid-liquid separation to obtain hemicellulose hydrolysate and a pretreated solid component; then saccharifying and pretreating the solid component by using cellulase to obtain hydrolysate rich in glucose, adsorbing the hemicellulose hydrolysate by using hypercrosslinked adsorption resin, carrying out solid-liquid separation to obtain hydrolysate rich in xylose, and carrying out desorption treatment on the resin by using industrial ethanol to obtain xylose; and performing rotary evaporation on the desorbed liquid to remove the industrial ethanol to obtain p-toluenesulfonic acid. According to the method, hemicellulose is efficiently degraded to obtain derived sugar of hemicellulose, meanwhile, the saccharification efficiency of cellulase is improved, p-toluenesulfonic acid is recycled through the hypercrosslinked adsorption resin, and the method has the advantages of being high in fermentable sugar yield, recyclable in acid catalyst, low in cost, environmentally friendly and the like.

The invention discloses a preparation method and application of anti-photoaging collagen polypeptide. The preparation method comprises the following steps: unfreezing and cleaning silver carp skin, airing and cutting; naHCO3 is added, and soaking is carried out; adding Na2CO3 and carrying out ultrasonic impurity removal; performing ultrasonic-assisted treatment; adding protease for enzymolysis treatment; establishing an enzymolysis kinetic model under the alkaline protease condition; standing, cooling and centrifuging the enzymatic hydrolysate, collecting supernate, filtering, and performing vacuum freeze drying to obtain faint yellow SCSCP; the light aging resistant peptide SCSCP02 is separated, and the active peptide sequences of the light aging resistant peptide SCSCP02 are GPPGPPGTPGPQ, SGLPGPIGPPGPR and GLPGPIGPPGPR. The active substances which are extracted from the fish skin and have the anti-photoaging effect can reduce the risk of skin light damage caused by UVR by acting on various treatment targets, resource waste can be reduced, environmental pollution caused by biological resources can be relieved, and sustainable development of the industry can be promoted.

The invention relates to the technical field of elastin peptides, in particular to a method for extracting elastin peptides from animal cartilage, which comprises the following specific steps: removing surface connective tissues and fat from animal cartilage, cleaning with normal saline, grinding and degreasing to obtain pretreated cartilage particles; putting the pretreated cartilage particles into a container, adding ionic liquid and stirring; after stirring is completed, centrifugally collecting precipitates; adding the precipitate into a phosphate buffer solution to form a suspension; adding an immobilized compound enzyme, and carrying out an enzymolysis reaction; after the enzymolysis is completed, raising the temperature for inactivation; filtering to obtain enzymatic hydrolysate; adding macroporous resin into the enzymatic hydrolysate, and filtering to obtain clear liquid; and carrying out ultrafiltration on the clear liquid to obtain the elastin peptide. The ionic liquid interacts with impurities in the animal cartilage, so that the impurities are easier to remove, a purer raw material environment is created for subsequent elastin peptide extraction, and the extraction efficiency is improved.

The invention discloses a hexapeptide with a hypoglycemic effect as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The amino acid sequence of the hexapeptide is Ile-Trp-Asp-Pro-His-Phe, and the amino acid sequence of the hexapeptide is as shown in the specification. The novel hexapeptide IWDPHF with prominent DPP-IV inhibition ability is successfully identified from mulberry leaf proteolysis products through liquid chromatography-mass spectrometry and a virtual screening method, the hexapeptide IWDPHF shows the blood glucose reducing effect superior to that of part of known peptides in an in-vitro enzyme activity inhibition experiment and a zebra fish hyperglycemia model, and no obvious side effect exists; the compound has a good potential of being developed into a hypoglycemic functional product or a drug lead compound. The invention further provides the mulberry leaf peptide with the peptide fragment IWDPHF, and the mulberry leaf peptide can be applied to preparation of drugs or food for reducing blood sugar. The invention provides a new scheme and theoretical basis for treatment of diabetes, and has good market prospect and application potential.

The present invention discloses a natural composite flavor enhancer and a preparation method thereof, comprising the following steps: using an ultramicro grinder to crush rice wine lees and wheat gluten powder to obtain ultramicro wheat gluten powder and ultramicro rice wine lees powder; taking the ultramicro wheat gluten powder, sterile water, and protease, mixing them evenly, carrying out enzymatic hydrolysis, inactivating the enzyme after the reaction ends, filtering, and taking the filtrate, namely the wheat gluten powder enzymatic hydrolysate, for standby; taking the wheat gluten powder enzymatic hydrolysate, ultramicro rice wine lees powder, and sterile water, mixing them evenly, carrying out enzymatic hydrolysis, inactivating the enzyme after the reaction ends, filtering, and taking the filtrate, namely the composite enzymatic hydrolysate, for standby; taking the composite enzymatic hydrolysate, cysteine, xylose, glucose, granulated sugar, and salt, mixing them and carrying out a thermal reaction to obtain a reaction solution; and preparing the natural flavor enhancer by spray drying. The present invention utilizes the natural enzyme system in rice wine lees to synergistically enzymatically hydrolyze wheat gluten powder to promote the release of umami amino acids, and further strengthens the umami by using thermal reaction technology.

The invention relates to the technical field of biology, in particular to a DPP-IV inhibitory peptide extracted from buffalo cheese protein hydrolysate and application of the DPP-IV inhibitory peptide. An active component with the molecular weight smaller than 3 kDa is obtained through alkaline protease enzymolysis, ultrafiltration separation and LC-MS / MS identification, and the DPP-IV enzyme inhibitory activity IC50 value of the active component is 12.6 mg / mL. Four new DPP-IV inhibitory peptide fragments are found, are amino acid sequences in a sequence table SEQ ID NO: 1-4, have good DPP-IV inhibitory activity, and have good application prospects in being developed into functional foods or medicines for regulating blood sugar. In conclusion, the enzymolysis product and the peptide fragment can be prepared into antidiabetic drugs or functional foods, and have the advantages of being natural, safe and efficient. The invention provides a new strategy for high-valued utilization of buffalo milk resources and treatment of type 2 diabetes.

The invention belongs to the technical field of biology, and particularly relates to a method for producing xylitol through two-step enzyme catalysis of hemicellulose hydrolysate, and the method comprises the following steps: taking hemicellulose hydrolysate as a raw material, adding arabinose dehydrogenase, NADH oxidase and coenzyme NADH, and carrying out first-step fermentation until no L-arabinose residue exists in fermentation liquor to obtain fermentation reaction liquor I; and adding xylose reductase and glucose dehydrogenase into the fermentation reaction liquid I, and carrying out second-step fermentation to obtain a fermentation product containing xylitol. According to the two-step enzyme catalysis method provided by the invention, xylitol is prepared, and high-purity xylose does not need to be used as a raw material; according to the present invention, the cheap hemicellulose hydrolysate can be directly adopted as the raw material, the xylose conversion rate and the xylitol yield can exceed 99%, the impurity arabitol content in the product is low, the other impurity saccharic acid is easy to separate, and the problems of complex process, large separation difficulty and high cost in the xylitol refining process are successfully solved.

The utility model relates to a system for reducing the acid value of hydrolysate in the separation and washing process of dimethyl dichlorosilane hydrolysate. Comprising three stages of coalescers, dimethyl dichlorosilane acidic hydrolysate enters the system from the first stage of coalescer, process water enters the system from the third stage of coalescer, the acidic hydrolysate and diluted hydrochloric acid or process water are mixed in each stage of coalescer, oil-water two-phase separation is completed through multiple times of washing and coalescence, and meanwhile the acid value of the hydrolysate is reduced; each stage of coalescer comprises two static mixers connected in series, a group of prefilters and a large coalescer, the coalescer is Z-shaped, and four stages of filter elements are arranged in each stage of coalescer. Through repeated tests, a modified polyvinylidene fluoride filter element of Pall Company is finally selected, and after continuous three-stage coalescer treatment, the acid value of dimethyldichlorosilane hydrolysate can be reduced to 10 PPM or below.

[Problem] To provide: metal oxide particles that can be highly concentrated in an organic solvent, which is a dispersion medium for a metal oxide sol, and can also be redispersed in the organic solvent; a metal oxide sol containing the metal oxide particles; and a method for producing same. [Solution] Metal oxide particles surface-coated with a hydrolysate of a silane compound (A) having two chemical groups (a1) and two hydrolyzable groups (a2), wherein the metal oxide particles contain a basic compound (I) (excluding ammonia), have a hydrophobicity of 30-80 vol% as measured by a methanol titration method, and can be dispersed at a particle refractive index of 1.4-3.0 and at a metal oxide concentration of at least 40 mass% in an organic solvent, or can be dispersed at a particle refractive index of at least 1.1 and less than 1.4 and at a metal oxide concentration of at least 25 mass% in an organic solvent.

The invention discloses a method for preparing superfine high-purity germanium dioxide from germanium tetrachloride. The method comprises the following steps: step 1, preparing a reactor; step 2, preparing hydrolysis mother liquor; step 3, hydrolysis; step 4, suction filtration and washing; step 5, drying; and step 6, screening. According to the method for preparing the superfine high-purity germanium dioxide, the production efficiency of the superfine high-purity germanium dioxide can be effectively improved, and the superfine high-purity germanium dioxide is efficiently prepared by controlling the hydrolysis ratio, the acidity of hydrolysate, the germanium content, the temperature of the whole hydrolysis process, the hydrolysis rate and the stirring power.

The invention relates to a preparation method of a perigord truffle extract as well as a product and application of the perigord truffle extract. The preparation method comprises the following steps: mixing perigord truffle with an enzyme solution for enzymolysis to obtain enzymatic hydrolysate; and mixing the enzymatic hydrolysate with an extracting agent, treating in a pulsed electric field, extracting, centrifuging, taking supernate, and filtering to obtain the traditional Chinese medicine composition. The perigord truffle extract is prepared through a specific extraction method, and the prepared perigord truffle extract has good antioxidant and anti-inflammatory effects.

This invention relates to the technical field of deep processing of sturgeon by-products, and particularly to a sturgeon tendon peptide with antioxidant and pancreatic lipase inhibitory activities and its preparation method. The preparation method includes the following steps: mixing sturgeon with water and crushing it to form a first slurry; adding Bacillus licheniformis alkaline protease to the first slurry for a first hydrolysis; adding Bacillus subtilis neutral protease to the first hydrolysate for a second hydrolysis; adding a complex enzyme to the second hydrolysate for a third hydrolysis to obtain a sturgeon tendon hydrolysate; filtering the sturgeon tendon hydrolysate to remove impurities to obtain a filtrate, which is then filtered again to retain polypeptides, thus obtaining the sturgeon tendon peptide; wherein the complex enzyme includes pectinase and amylase. Using this method, a sturgeon tendon peptide with high glycosaminoglycan content can be obtained, which exhibits pancreatic lipase inhibitory activity, antioxidant activity, Staphylococcus aureus inhibitory activity, and prebiotic effects that promote the proliferation of Lactobacillus plantarum and Lactobacillus fermentum.

The invention provides a whey protein deep hydrolysate with low sensitization and an allergy relieving effect as well as a preparation method and application of the whey protein deep hydrolysate. According to the method, the alkaline protease, the neutral protease and the glutamyltransferase are sequentially used for carrying out first enzymolysis, second enzymolysis and third enzymolysis on the whey protein raw material, and the whey protein hydrolysate which is low in molecular weight, good in taste, good in digestion and absorption performance, low in sensitization and capable of effectively relieving allergic reaction of the organism is obtained.

The invention discloses a preparation method for simultaneously and efficiently extracting crocodile polypeptide and crocodile oil, and relates to the technical field of biological extraction, crocodile meat is used as a raw material, and heating micro-pressure treatment is sequentially performed to promote component dissolution; a multi-stage enzymolysis process is adopted, alkaline protease, neutral protease and lipase are sequentially used, and a calcium phosphate system is used for assisting filtration and a natural antioxidant is added in a gradient manner in the enzymolysis process; performing plate frame filtration and standing layering subsequently to realize crude separation of the oil peptide; finally, the crocodile polypeptide liquid is subjected to multi-stage membrane filtration and spray drying, the crocodile crude oil is subjected to ultrasonic-assisted fishy smell removal, ceramic membrane fine filtration and gradient cooling phase change refining, and after enzymolysis treatment and before filtration, a composite additive is added into enzymatic hydrolysate and stirred. No organic solvent exists in the whole process, and the product yield, purity and oxidation stability are remarkably improved.

The invention discloses a radix pseudostellariae extract with high content of radix pseudostellariae cyclic peptide B and a preparation method and cosmetic application of the radix pseudostellariae extract, and belongs to the technical field of cosmetics. The preparation method comprises the following steps: (1) crushing radix pseudostellariae, adding alcohol and performing ultrasonic treatment to obtain feed liquid; (2) adding a compound enzyme into the feed liquid to carry out enzymolysis, enzyme deactivation, solid-liquid separation, concentration and impurity removal to obtain an enzymatic hydrolysate; and (3) eluting the enzymatic hydrolysate with macroporous resin to obtain an eluent, and drying to obtain the product, the compound enzyme is prepared from pectinase, papain, beta-glucosidase, cellulase and xylanase according to the mass ratio of (5 to 10) to (0.5 to 1) to (3 to 7) to (0.3 to 1). The radix pseudostellariae extract disclosed by the invention is prepared by adopting an enzymolysis process, more active ingredients can be reserved, the content of radix pseudostellariae cyclic peptide B in the obtained extract is high, and the radix pseudostellariae extract has relatively good anti-inflammatory, soothing, repairing and anti-aging effects.

The invention discloses a screening method of hericium erinaceus functional polypeptide based on artificial intelligence assistance and polypeptide or a polypeptide composition. The method comprises the following steps: (1) constructing an AI auxiliary annotation model; (2) extracting hericium erinaceus crude protein; (3) preparing hericium erinaceus protein enzymatic hydrolysate through composite enzymatic hydrolysis; (4) performing polypeptide identification on the hericium erinaceus protein enzymatic hydrolysate to obtain a polypeptide sequence; and (5) carrying out AI auxiliary annotation and polypeptide screening by using a large language model based on a DeepSeek platform so as to obtain a polypeptide sequence with assumed biological activity. The polypeptide sequence and the polypeptide composition which have remarkable ACE inhibition and oxidation resistance dual functions are successfully obtained by utilizing the method disclosed by the invention. Compared with a traditional experience screening path, the method realizes a high-throughput active peptide development process which is targeted in structure, accurate in prediction and capable of verifying a closed loop.

The invention relates to a preparation method of small-molecular-weight sea cucumber oligopeptide, the prepared sea cucumber oligopeptide and application, and belongs to the technical field of skin care products. The preparation method of the small-molecular-weight sea cucumber oligopeptide comprises the following steps: preparing sea cucumber fine powder; adding the sea cucumber fine powder into ultrapure water, adding papain and EDTA (Ethylene Diamine Tetraacetic Acid), and carrying out enzymolysis under the assistance of microwaves; adding trypsin and calcium ions, and carrying out enzymolysis under the assistance of microwaves; adding neutral protease and zinc ions, and performing enzymolysis under the assistance of microwaves to obtain final sea cucumber enzymatic hydrolysate; and decolorizing, separating and purifying, and freeze-drying to obtain the powdery small-molecular-weight sea cucumber oligopeptide. According to the preparation method of the small-molecular-weight sea cucumber oligopeptide, microwave heating and enzymolysis reaction are combined, the hydrolysis degree of a peptide chain is regulated and controlled by controlling an enzyme hydrolysis sequence and enzymolysis conditions, the small-molecular-weight sea cucumber oligopeptide with relatively concentrated and moderate molecular weight is prepared, and good biological activity of the sea cucumber oligopeptide is kept.

The invention discloses a preparation method of fresh taxus chinensis stem and leaf protoplasts. The preparation method comprises a preparation process and a purification process. The preparation method of the taxus chinensis stem and leaf protoplast is simple and easy to operate, raw materials are easy to obtain and low in price, reagent components have good biocompatibility, and the taxus chinensis stem and leaf protoplast is free of harmful components, safe and environmentally friendly. The invention provides a simple and rapid taxus chinensis stem and leaf protoplast enzymolysis preparation method, which adopts vacuum filtration, accelerates the permeation of enzymatic hydrolysate and improves the enzymolysis efficiency, so that the protoplast can be rapidly obtained. Meanwhile, high-purity separation of the protoplast is carried out in combination with density gradient sedimentation of the iodixanol solution, so that the protoplast with uniform size and complete form is obtained, and convenience is provided for scientific researches such as subsequent conversion and single cell transcriptome sequencing.

The invention discloses a nutritional composition for promoting neurodevelopment and application, and provides a nutritional composition for promoting neurodevelopment, which comprises the following necessary components: a sialylated substance component comprising one or more of N-acetylneuraminic acid, sialylated oligosaccharide, sialylated protein and sialylated lipid; the whey protein component comprises alpha-lactalbumin; and a peptide component from casein, including a hydrolysate of casein; the nutritional composition comprises a sialylated substance component, a whey protein component and a casein-derived peptide component, and the mass ratio of the sialylated substance component, the whey protein component and the casein-derived peptide component in the nutritional composition is (0.5-10): (300-1): (100-0.5). The nutritional composition provided by the invention can promote brain development and cognitive functions, and can significantly promote neurodevelopment by scientifically matching the sialylated substance component, the whey protein component and the peptide component from casein.

The present disclosure relates to processes for producing hydrocarbon fuels from lignocellulosic biomass. A process may include introducing biomass to a pretreatment system, and a first separation system forming a pentose-rich stream and a pentose-lean stream. The pentose-lean stream may be introduced to a hydrolysis system forming a hydrolysate and the hydrolysate introduced to a second separation system forming a hexose-rich stream and a lignin stream. Additionally, at least one of the pentose-rich stream or the hexose-rich stream may be introduced to a bioreactor containing microorganisms configured to produce hydrocarbon fuels. Additionally, the present disclosure also relates to systems for the production of hydrocarbon fuels. A system may include a pretreatment system, a first separation system, a hydrolysis system, a second separation system, and one or more bioreactors. Alternatively a system may include a pretreatment system, a hydrolysis system, a sugar separation system, and one or more bioreactors.

The invention belongs to the technical field of functional food processing, and particularly relates to a method for regulating peptide flavor and digesting characteristics by utilizing casein peptide and polyphenol self-assembly. According to the research, the casein peptide with good GABA-T inhibitory activity is taken as a raw material, and ferulic acid is added to be self-assembled with the casein peptide, so that the bitter taste of the casein peptide is reduced, and the GABA-T inhibitory activity of the casein peptide is improved in an in-vitro digestion stage. Compared with a traditional zymolyte debitterizing method, the method has the advantages that the biological activity of peptide is reserved, no toxic chemical reagent is introduced or added, and the method is safer, more efficient, more environmentally friendly and more sustainable. The method can be widely applied to functional food or health care products.

The invention belongs to the technical field of marine biological medicines, and relates to a hippocampus polypeptide for improving cognitive impairment of Alzheimer's disease and a preparation method and application thereof.The preparation method comprises the steps that hippocampus powder is dissolved in water, the pH value is adjusted to 1.8-2.5, pepsin is added for oscillation incubation for 2 hours, and then high-temperature heating is conducted to inactivate enzyme; adjusting the pH value to 7.3-8.0, adding chymotrypsin to form a compound enzyme enzymolysis combination, oscillating and incubating for 2 hours, and heating at high temperature to inactivate the enzyme; centrifuging the solution, collecting enzymatic hydrolysate, and filtering the obtained hippocampus crude polypeptide through an ultrafiltration membrane to obtain molecular weight lt; the hippocampus polypeptide is subjected to vacuum freeze drying and preservation. The hippocampus polypeptide extracted and prepared from hippocampus japonicus by adopting a biological enzymolysis method has excellent APP inhibitory activity, can remarkably improve dysmnesia, and can be used for developing related functional foods or medicines for treating Alzheimer's disease.

The invention provides a plant exosome as well as an extraction method and application thereof in cosmetics, and belongs to the technical field of exosome extraction. The preparation method of the plant exosome comprises the following steps: mixing plant tissues with a solvent, carrying out high-pressure homogenization treatment, adding immobilized compound enzyme for enzymolysis, filtering, carrying out differential centrifugation on enzymatic hydrolysate, and carrying out size exclusion chromatography and ultrafiltration purification to obtain the plant exosome. Wherein exosome release and protection are considered, and the influence of agglomeration on the exosome extraction effect is avoided; high-pressure homogenization and immobilized compound enzyme enzymolysis are adopted, physical wall breaking is combined with chemical wall breaking, and the yield of the exosome is increased; the immobilized compound enzyme can avoid free enzyme residues, and the subsequent purification step is simplified; the plant exosome can be further purified through size exclusion chromatography and ultrafiltration. The exosome prepared by the method disclosed by the invention can retain the active ingredients of the exosome to the greatest extent, has excellent antioxidant, anti-inflammatory and whitening effects, and is suitable for the field of cosmetics.

The invention belongs to the technical field of collagen peptide extraction, and discloses a preparation process of low-fishy-smell marine fish skin collagen peptide, which is used for solving the problems of incomplete fishy smell removal and high protein loss rate in the traditional fishy smell removal process. Comprising the following steps: (1) immersing fish skin into water, adding lipase, cleaning the fish skin with pure water, and homogenizing to obtain homogenate; (2) adding saccharomyces cerevisiae and purple perilla juice into the homogenate, carrying out heat preservation and enzymolysis at 30 DEG C, then heating to 45 DEG C, adding compound enzyme for enzymolysis, and heating for enzyme deactivation to obtain collagen peptide enzymatic hydrolysate; (3) adding activated carbon and diatomite into the collagen peptide enzymatic hydrolysate, keeping the temperature at 70 DEG C, stirring, and carrying out filter pressing and refined filtration to obtain a collagen peptide decoloring solution; (4) sterilizing and concentrating the collagen peptide decoloring solution to obtain a collagen peptide concentrated solution; and (5) drying the collagen peptide concentrated solution to obtain collagen peptide powder. The collagen peptide prepared by the method is almost free of fishy smell and low in protein loss.