726 results about "Hemicellulose" patented technology

The invention discloses a deep eutectic solvent / bimetal organic framework co-catalyzed straw polyol as well as a preparation method and application thereof, the straw polyol is prepared by the following method: uniformly mixing straw powder, small molecular alcohol, an acid catalyst and a deep eutectic solvent in a nitrogen atmosphere, and reacting to obtain a reaction product; and adding a bimetal organic framework catalyst into the reaction product, and reacting to obtain the straw polyol. A green, natural and pollution-free eutectic solvent catalytic liquefaction technology is adopted, lignin and hemicellulose can be dissolved, the cross-linking effect between lignin and hemicellulose and cellulose is reduced, the crystalline structure of cellulose can be destroyed, the available surface area is increased, the contact with a liquefying agent is enhanced, the conversion efficiency is improved, and the residue rate is reduced; the bimetallic organic framework catalyst is added in the middle and later periods of the reaction, aldehydes, ketones and acid and ester substances can be reduced and upgraded into alcohols, the hydroxyl value of the liquefied polyol is greatly increased, and the film-forming crosslinking degree is improved.

The invention discloses a rice straw decomposition process full life cycle monitoring system based on a cloud platform, and relates to the field of agricultural informatization, and the system comprises a field data collection terminal which is used for collecting near infrared spectrum data, image data and operation and environment data of a straw decomposition process; the layered identification and modeling unit is used for identifying initial mass proportions and decomposition progress of soluble components, cellulose components, hemicellulose components and lignin components of the straws, and establishing a layered progress bar; the fusion progress calculation unit is used for weighting each layered progress bar based on the initial mass ratio to generate an overall decomposition progress bar; the progress output unit is used for simultaneously outputting a layered progress bar and an overall decomposition progress bar; and the mode mining unit is used for clustering the historical layered progress bars, forming a progress category prototype library and outputting potential blocking reasons. The problems that the field straw decomposition process is difficult to monitor in real time and the blocking reason is difficult to identify are solved.

The invention discloses a preparation method for treating bagasse to obtain hard carbon, application of the hard carbon and a sodium-ion battery negative electrode, and relates to the field of sodium-ion batteries. The preparation method of the bagasse-based hard carbon comprises the following steps: crosslinking lignin and cellulose in the bagasse-based hard carbon by using an acid solution, and partially stripping ash of biomass. And stripping hemicellulose of the bagasse by using a weak base solution to expose cellulose. By regulating and controlling the concentration, the treatment time and the treatment temperature of the alkali solution, reasonable lignin is reserved in the cellulose, so that regulation and control of a plain pore structure in the carbonization process are promoted. After the alkali solution treatment, a hydrochloric acid solution is used for cleaning the solid, and freeze drying is carried out, so that a proper pore structure is reserved. And then carrying out high-temperature heat treatment in a protective atmosphere, wherein the heat treatment is carried out in three stages. The reaction process is a cellulose deoxidation process, the treatment temperature is 300-400 DEG C, and primary carbonization of cellulose is realized; secondly, carbonizing, namely further deoxidizing after primary carbonization, wherein a large number of C = C / C-C bonds are formed at the treatment temperature of 800-1000 DEG C; and finally, forming a hard carbon graphite layer, wherein the treatment temperature is 1300-1500 DEG C. The bagasse-based hard carbon prepared by the preparation method can be efficiently and stably circulated in a sodium ion battery, and shows excellent rate capability.

The invention discloses a method for preparing fermentable sugar from recyclable low-concentration p-toluenesulfonic acid pretreated lignocellulose, which comprises the following steps: firstly, pretreating lignocellulose by using a low-concentration p-toluenesulfonic acid aqueous solution, and carrying out solid-liquid separation to obtain hemicellulose hydrolysate and a pretreated solid component; then saccharifying and pretreating the solid component by using cellulase to obtain hydrolysate rich in glucose, adsorbing the hemicellulose hydrolysate by using hypercrosslinked adsorption resin, carrying out solid-liquid separation to obtain hydrolysate rich in xylose, and carrying out desorption treatment on the resin by using industrial ethanol to obtain xylose; and performing rotary evaporation on the desorbed liquid to remove the industrial ethanol to obtain p-toluenesulfonic acid. According to the method, hemicellulose is efficiently degraded to obtain derived sugar of hemicellulose, meanwhile, the saccharification efficiency of cellulase is improved, p-toluenesulfonic acid is recycled through the hypercrosslinked adsorption resin, and the method has the advantages of being high in fermentable sugar yield, recyclable in acid catalyst, low in cost, environmentally friendly and the like.

The invention discloses a biomass-based hard carbon negative electrode material with high specific capacity and high magnification as well as a preparation method and application of the biomass-based hard carbon negative electrode material. The method comprises the following steps: pretreating lignocellulose biomass by a hot pressing method, and carbonizing in a protective gas atmosphere to obtain the hard carbon material. A hot-pressing pretreatment process adopted by the invention can decompose part of hemicellulose to obtain a precursor rich in lignin and cellulose, so that the thermal stability of the material is improved. In the process, the lignin can effectively inhibit the rapid centralized pyrolysis of the cellulose and weaken the rearrangement of the pyrolysis product of the cellulose. The method not only helps to prevent overgrowth of hard carbon graphite microcrystals during high-temperature carbonization, but also promotes generation of a small-size closed-pore structure, so that the microstructure of the hard carbon material is optimized. The hard carbon material shows high specific capacity and excellent rate capability when being applied to a sodium-ion battery negative electrode. The method has the advantages of simplicity in operation, low requirements on equipment, no need of adding chemical reagents, greenness, environmental protection and the like.

The invention discloses a method for preparing high-quality dissolving pulp from low-grade plant fiber raw materials, and belongs to the technical field of biomass pulping. The preparation method comprises the following steps: screening a low-grade plant fiber raw material to obtain a fiber raw material of which the length is 20-30 mm, the width is 10-15 mm and the thickness is 3-5 mm; carrying out deep prehydrolysis-sulfate cooking treatment on the screened fiber raw materials to obtain unbleached sulfate pulp fibers; performing deep eutectic solvent and elemental chlorine-free bleaching treatment on the unbleached sulfate pulp fiber to obtain paper pulp fiber with low kappa number; and carrying out biological enzyme treatment on the paper pulp fibers with low kappa number to obtain the high-quality dissolving pulp. According to the method, the morphology and the structure of chemical pulp fibers are effectively regulated and controlled by designing deep eutectic solvent treatment, and deep dissolution of residual hemicelluloses and lignin is achieved; through biological enzyme treatment, the alpha-cellulose content and the Fock reaction performance are further improved, and finally the high-quality dissolving pulp is prepared.

The invention belongs to the technical field of biology, and particularly relates to a method for producing xylitol through two-step enzyme catalysis of hemicellulose hydrolysate, and the method comprises the following steps: taking hemicellulose hydrolysate as a raw material, adding arabinose dehydrogenase, NADH oxidase and coenzyme NADH, and carrying out first-step fermentation until no L-arabinose residue exists in fermentation liquor to obtain fermentation reaction liquor I; and adding xylose reductase and glucose dehydrogenase into the fermentation reaction liquid I, and carrying out second-step fermentation to obtain a fermentation product containing xylitol. According to the two-step enzyme catalysis method provided by the invention, xylitol is prepared, and high-purity xylose does not need to be used as a raw material; according to the present invention, the cheap hemicellulose hydrolysate can be directly adopted as the raw material, the xylose conversion rate and the xylitol yield can exceed 99%, the impurity arabitol content in the product is low, the other impurity saccharic acid is easy to separate, and the problems of complex process, large separation difficulty and high cost in the xylitol refining process are successfully solved.

The invention discloses a method for value-added utilization of straw biomass and extraction and purification of fulvic acid and fulvic acid derivatives of the straw biomass, and belongs to the field of resource utilization of agricultural and forestry wastes. Through regulation and control of raw materials in feed liquid, fulvic acid, vegetable protein and other organic matters can be efficiently separated from three elements in straw, cellulose and hemicelluloses are further efficiently separated from lignin, and then fulvic acid and derivatives thereof are efficiently separated from other organic matters and purified through an extraction separation method. The functionalization degree and the functional group type of the fulvic acid or the fulvic acid derivative thereof are regulated and controlled according to different components of the added cooking liquor, the high-purity fulvic acid or the new derivative thereof is obtained, and other organic matters containing protein and the like can be used as natural organic fertilizers; meanwhile, the obtained three elements are subjected to secondary cooking treatment, and lignin can be effectively separated and purified from cellulose and hemicellulose by regulating and controlling components of cooking liquor. And finally, the straw proliferation waste-free efficient utilization is realized.

The invention discloses an element liquid fertilizer for promoting nutrient absorption and a preparation method thereof, and belongs to the technical field of fertilizers. Comprising monoammonium phosphate; urea; potassium nitrate; cake fertilizer leach liquor; trace elements; a wetting agent; a stabilizer; a biological agent; an adsorbent; deionized water; the preparation method of the adsorbent comprises the following steps: A1, taking loofah sponge powder, and removing lignin and hemicellulose to obtain loofah sponge cellulose powder; a2, mixing the loofah sponge cellulose powder, deionized water, a chitosan solution and plant ash, mechanically pulping uniformly, performing vacuum freeze-drying treatment to form holes, crushing and grinding to obtain the adsorbent, root development can be promoted, elongation and thickening of stems are promoted, the photosynthetic rate of plants is increased, and the crop yield is increased.

The invention discloses bacillus subtilis capable of producing cellulase at high yield and application of the bacillus subtilis, and belongs to the technical field of biology, the bacillus subtilis is classified and named as bacillus subtilis KC7-2, the bacillus subtilis KC7-2 is preserved in China Center for Type Culture Collection on July 15, 2024, and the preservation number is CCTCC NO: M20241570. The bacillus subtilis provided by the invention shows excellent performance in the aspect of secretion of cellulase and hemicellulase, especially reaches an enzymatic activity peak in the early growth stage (24h) of a strain, and can maintain a certain level of enzymatic activity for a long time later. Due to the characteristics, the strain has important application value in the fields of forage feed improvement, biomass degradation, environmental protection and the like.

The invention discloses a preparation method of a lignin-coated cellulose nanofiber sodium-ion battery diaphragm, and relates to the technical field of electrochemistry and battery diaphragms.The preparation method comprises the steps that bamboo particles are pretreated through a hydrothermal method; then carrying out bamboo particle nanocrystallization by adopting a mechanical dissociation method to obtain a lignin-coated cellulose nanofiber suspension, and preparing the lignin-coated cellulose nanofiber into a thin film by adopting a vacuum filtration method. The preparation process of the cellulose coated with the lignin nanoparticles is direct, simple, efficient and environment-friendly, chemical reagents such as acid and alkali do not need to be adopted for removing hemicellulose and lignin, meanwhile, the diffusion rate of sodium ions in the charging and discharging process is effectively increased due to coating of the lignin nanoparticles, and the sodium ion conductivity is improved. The specific discharge capacity and the cycling stability are realized.

The invention discloses a Coaerella coagulum-plant lactobacillus synergistic fermentation inoculant and a process for staged solid-state fermentation of American ginseng by using the same, and belongs to the technical field of biological fermentation. According to the invention, two strains, i.e., the plant lactobacillus and the hainerella coagulatum, are obtained by screening food-grade probiotic strains, and the ginsenoside conversion capability of the two strains is verified by utilizing culture. The invention also provides a probiotic fermentation process for improving the content of rare ginsenoside in American ginseng, namely, the staged synergistic fermentation of the Halidernia coagulum and the phytobacterium plantarum is utilized, so that the content of the rare ginsenoside in the American ginseng is improved; cellulase and hemicellulase secreted by the Haihdrikia coagulatum can decompose cellulose, lignin and the like in cell walls of the surface layer of the American ginseng raw material in the early stage of fermentation, so that ginsenoside in cells is fully released, and beta-glucosidase from different sources is utilized to synergistically convert prototype ginsenoside in the later stage of fermentation. The content of various rare ginsenosides in American ginseng can be remarkably increased through the solid-state fermentation process. Meanwhile, the solid-state fermentation process has the advantages of mild conditions, less wastewater treatment, environmental protection, low cost, process safety and the like, has important significance in improving the resource utilization of American ginseng and promoting the industrial development of American ginseng, and has relatively good practicability.

The invention relates to a method for extracting and purifying lycium barbarum polyphenol by combining bacteria and enzymes. The method comprises the following steps: pretreating lycium barbarum fruits to obtain lycium barbarum powder; extracting the wolfberry powder by using a first solvent to obtain a first crude extract; fermenting the first crude extract with lactobacillus plantarum to obtain a second crude extract; performing enzymolysis on the second crude extract by using pectinase, hemicellulase and beta-glucosidase in sequence to obtain an enzymolysis extracting solution; adsorbing and purifying the enzymolysis extracting solution by using first ion exchange resin, washing and eluting to obtain a first eluent; adsorbing and purifying the first eluent by using second ion exchange resin, washing and eluting to obtain a second eluent; and concentrating and drying the second eluent to obtain wolfberry polyphenol powder. According to the method disclosed by the invention, the fermentation of lactobacillus plantarum is combined with the synergistic effect of pectinase, hemicellulase and beta-glucosidase, polysaccharide in a crude extract is decomposed, polyphenol is released and combined, the extraction rate and the purity of the polyphenol are improved, and meanwhile, a continuous purification process of macroporous resins AB-8 and D-101 is combined, so that the product quality is further improved.

The present invention provides a deep eutectic solvent and its preparation and application. The deep eutectic solvent comprises choline chloride, p-toluenesulfonic acid, 1,4-butanediol, and water; wherein the molar ratio of choline chloride, p-toluenesulfonic acid, and 1,4-butanediol is 1-2:3-6:2-8, and the ratio of the total mass of choline chloride and p-toluenesulfonic acid to the mass of water is 20:9-11. When used for the pretreatment of Miscanthus sinensis, the deep eutectic solvent of the present invention can effectively remove hemicellulose and lignin from the cell walls of Miscanthus sinensis, thereby significantly improving the efficiency of its enzymatic hydrolysis of cellulose.

The invention belongs to the technical field of biological fermentation hydrogen production. The invention provides a method for producing hydrogen by dark fermentation of pericarp biomass, which comprises the following steps: drying pericarp, crushing, and sieving to obtain powdery pericarp particles; performing acid heat pretreatment on the powdery fruit peel particles to obtain fruit peel pretreatment liquid; and inoculating the clostridium butyricum solution into the pericarp pretreatment liquid, and carrying out dark fermentation under an anaerobic condition to obtain a fermentation gas containing hydrogen. According to the method, the pericarp byproducts are used for dark fermentation for hydrogen production, so that high-value recycling utilization of agricultural wastes is realized; lignin in the pericarp is effectively removed through acid heat pretreatment, the availability of cellulose and hemicellulose is remarkably improved, conditions such as acid concentration, temperature and time are optimized, the inhibition effect of by-products is avoided, and the hydrogen production efficiency is remarkably improved; moreover, the method has the advantages of good stability and high repeatability, and provides a new technical approach for efficient production of clean energy.

The invention discloses a method for preparing viscose fiber from alkali-soaked hemicellulose, and relates to the technical field of viscose fiber production and preparation, and the method comprises the following steps: sequentially carrying out multi-stage filtration and twice nanofiltration membrane filtration on alkali liquor squeezed from slurry porridge, and separating out high-concentration hemicellulose alkali liquor; after cellulose xanthate is generated through a xanthating machine reaction, high-concentration hemicellulose alkali liquor is added into the xanthating machine to be subjected to a xanthating reaction, and hemicellulose xanthate is generated; and adding dilute alkali liquor into the xanthating machine for dissolving, and sequentially filtering, defoaming and spinning to prepare the viscose fiber. After alkali cellulose and carbon disulfide are subjected to a yellowing reaction, high-concentration hemicellulose alkali liquor is added into a yellowing machine for secondary yellowing, then two xanthate esters are dissolved with dilute alkali liquor, and subsequent filtering, defoaming and spinning procedures are performed, so that full utilization of hemicellulose is realized, and the quality and spinnability of viscose fibers are guaranteed; meanwhile, pulp unit consumption and new alkali consumption are reduced, and the production cost is saved.

The invention discloses a method for preparing phenol by utilizing a biomass raw material, which comprises the following steps: 1) crushing biomass, adding the crushed biomass into a caustic alkali solution to carry out hemicellulose removal treatment, and carrying out solid-liquid separation to take a solid substance, so as to obtain hemicellulose-removed biomass; 2) adding the hemicellulose-removed biomass into an ethylene glycol-ferric chloride eutectic solvent for lignin extraction, then adding an acetone-water mixed solution, then carrying out solid-liquid separation, taking a liquid substance, adding water to precipitate the lignin, then carrying out solid-liquid separation, and taking a solid substance to obtain the lignin; and 3) pyrolyzing the lignin in a protective atmosphere to obtain lignin oil containing phenol. The method for preparing phenol from biomass raw materials has the advantages of simple operation, high phenol yield, high product purity, low energy consumption, safety, environmental protection and the like, and is suitable for large-scale industrial application.

The invention provides a method for separating three major components of wood fiber biomass in one step by using a carbon dioxide-assisted two-phase solvent, and belongs to the technical field of lignocellulose treatment. The method comprises the following steps: placing an extraction solvent at the bottom of a reactor, and fixing a wood fiber biomass raw material at the upper part of the reactor; introducing carbon dioxide into the reactor, extracting under a closed condition, and introducing a cooling medium to adjust the temperature in the extraction process to obtain a solid-phase material, an organic phase and a water phase; the solid-phase material contains coarse cellulose, the organic phase contains lignin, and the water phase contains hemicellulose. The CO2 adopted by the method is non-corrosive, the aqueous solution of the CO2 is acidic, the generated H < + > can effectively promote the separation of wood fiber biomass components, and the CO2 can be recycled through pressure reduction and is circularly used for wood fiber raw material treatment.

The application belongs to the technical field of deep eutectic solvents, and particularly relates to a ternary deep eutectic solvent and preparation and application thereof. The ternary deep eutectic solvent is composed of choline chloride, 5-sulfosalicylic acid and gamma valerolactone according to a molar ratio of 1:4:(3-25). The strong hydrogen bond network structure between the green solvent system first destroys the ester bond between lignin and hemicellulose, then cuts off the beta-O-4 bond between lignin molecules, so as to separate lignin. Meanwhile, the deep eutectic solvent can improve the stability of carbon cations due to the conjugation effect between the lone pair electrons on the heteroatom oxygen and the central carbon in the molecular structure of gamma valerolactone, so as to inhibit the condensation reaction of lignin.

The invention discloses a method for improving moso bamboo cellulase hydrolysis conversion efficiency through amino acid-based deep eutectic solvent pretreatment, and belongs to the technical field of biomass recycling. The method comprises the following steps: mixing an L-cysteine solid and a formic acid solution, performing water bath heating to prepare an acidic eutectic solvent, performing microwave-assisted eutectic solvent pretreatment on moso bamboos, and performing filtration separation and enzymolysis; wherein the molar ratio of the L-cysteine to the formic acid is 1: (4-5). A deep eutectic solvent formed by formic acid and cysteine has a strong hydrogen bond network and an acid environment, beta-O-4 ether bonds in lignin are promoted to be broken, sulfydryl of cysteine participates in nucleophilic attack, a lignin-carbohydrate complex is further destroyed, formic acid can hydrolyze acetyl and glucosidic bonds in hemicellulose, and the lignin-carbohydrate complex is further destroyed. And degrading into an oligomer or monosaccharide. The phyllostachys pubescens components are efficiently separated, the cellulose enzymolysis saccharification capacity is improved, and effective utilization of all the phyllostachys pubescens components is facilitated.

The invention discloses a lignocellulose biomass-based hard carbon negative electrode material as well as a preparation method and application thereof. The method comprises the following steps: carrying out hydrothermal pretreatment on lignocellulose biomass by using low-corrosivity maleic acid, and regulating and controlling the composition of the lignocellulose biomass, the maleic acid and the lignocellulose to obtain a precursor rich in lignin and cellulose; in the pyrolysis and carbonization process, the thermal stability of the precursor is improved, and the lignin can more effectively inhibit the rapid concentrated pyrolysis of cellulose, so that the rearrangement of pyrolysis products is weakened. The method is beneficial for preventing overgrowth of hard carbon graphite microcrystals during high-temperature carbonization, so that the hard carbon material with large interlayer spacing and abundant small-size closed pores is prepared. The hard carbon material shows high specific capacity and excellent rate capability when being applied to the negative electrode of the sodium-ion battery. Meanwhile, xylose and furfural generated by decomposition of hemicellulose in a liquid phase can be effectively collected, and full-component utilization of biomass is achieved. The requirements of green production are met, and industrial application is easy to realize.

The invention belongs to the technical field of natural textile material modification, and particularly relates to a preparation method of bio-enzyme synergistic microorganism modified high-toughness mudar floss, which comprises the following steps: taking mudar floss as a raw material matrix, specifically providing clean mudar floss, and performing opening and activating treatment; performing solid-state fermentation treatment on the mudar floss by using brown rot fungi, specifically, inoculating activated brown rot fungi into the mudar floss for solid-state fermentation, and selectively degrading non-lignin impurities such as hemicellulose and the like; according to the preparation method of the high-toughness calotropis gigantea fiber modified by the bio-enzyme and the microorganisms, a biological method is adopted in the whole process, environmental pollution and fiber damage caused by traditional chemical modification are avoided, the process is mild and clean, a three-step synergistic strategy of brown rot fungus pretreatment, compound enzyme fine trimming and tannic acid toughening is innovatively adopted, and the method is suitable for industrial production. The brown rot fungi create a channel for subsequent enzyme reaction, enzyme treatment exposes active sites for tannic acid cross-linking, progressive layer by layer, high efficiency and remarkable effect are achieved.

The invention relates to the technical field of low-temperature microbial agents. The invention provides a bacterium-enzyme compound preparation for straw degradation and application and a use method thereof. The bacterium-enzyme compound preparation comprises a bacterium agent and an enzyme agent, the microbial agent comprises lactobacillus plantarum, bacillus subtilis, trichoderma viride and pichia guilliermondii; the enzyme agent comprises amylase, chitosanase, pectinase, cellulose, hemicellulase and lignin peroxidase. The use effect of the composite preparation is superior to the treatment effect of a single strain or an enzyme preparation, and straw can be efficiently degraded and converted in the low-temperature environment of 10-15 DEG C. The environmental limitation of cold regions is broken through, and the efficient degradation and conversion of the straws are realized. Meanwhile, the bacterium-enzyme synergism can effectively shorten the fermentation period, accelerate the composting effect of the fertilizer and improve the soil fertility and the microbial activity.

The invention belongs to the field of bioengineering, and particularly relates to a flexible mycelium composite material as well as a preparation process and application thereof. The flexible mycelium composite material disclosed by the invention is prepared by inoculating an edible fungus seed solution into a lignocellulose matrix, mixing, filling a mold and integrally fermenting, and the preparation process is simple. According to the method, rhamnolipid is added into an edible fungus seed solution culture medium, so that the enzyme activity of cellulase in the seed solution is effectively improved, the degradation and utilization of cellulose and hemicellulose in a lignocellulose matrix are further improved, the planting capacity of mycelia on the surface of lignocellulose is improved, the biomass of the mycelia is improved, and the yield of the mycelia is increased. Finally, the toughness of the flexible mycelium composite material is improved, so that the bending property of the flexible mycelium composite material is improved. Meanwhile, the flexible mycelium composite material prepared by the preparation method disclosed by the invention is biologically converted by utilizing agricultural and forestry wastes, the material utilization rate can reach 100%, and the flexible mycelium composite material is free from environmental pollution and has a wide industrial application prospect.

The invention belongs to the technical field of plant genetic engineering, particularly relates to application of a transcription factor CitNAC71 in regulation and control of synthesis of citrus withered water and juice sac secondary walls, and discloses application of the transcription factor CitNAC71 or a biological material for regulating and controlling expression of the transcription factor CitNAC71 in regulation and control of synthesis of citrus withered water and / or secondary walls, and the amino acid sequence of the transcription factor CitNAC71 is shown as SEQ ID NO: 1. The invention discovers that the gene expression level of the coding gene CitNAC71 of the transcription factor CitNAC71 has a significant negative correlation trend with the juice cell withering degree and the cellulose and hemicellulose content, and experiments prove that the CitNAC71 is a conservative fruit hardness regulation factor. The invention lays a theoretical foundation for preventing and controlling the occurrence of dry water before and after the harvesting of the citrus, and provides a potential target for improving the texture of the citrus fruit by a genetic engineering technology.

The invention discloses a potato peel polyphenol extraction method, which comprises: S1, crushing and screening potato peel, adding hemicellulase and pectinase according to a mass ratio of 1: 1, with the addition amount of the enzyme being 0.8-1.6% of the weight of the crushed and screened potato peel, and carrying out enzymolysis in a water bath at a temperature of 45-55 DEG C for 1.5-2.5 h; s2, an ethanol solution with the concentration of 50-70% is added, the material-liquid ratio is 1: 15-1: 25 g / mL, extraction is conducted for 30-60 min at the temperature of 55-65 DEG C, and then ultrasonic treatment is conducted for 15-30 min under the conditions that the power is 350-450 W and the temperature is 5-15 DEG C; and S3, carrying out rotary evaporation and constant volume on the extracting solution, and purifying by adopting macroporous adsorption resin to obtain the extract.

The invention belongs to the field of cellulose degradation strains, and particularly discloses bacillus subtilis and application thereof. The bacillus subtilis is classified and named as bacillus subtilis, the name of the bacillus subtilis is bacillus subtilis B-03, the bacillus subtilis is preserved in Guangdong Microbial Culture Collection Center on August 29, 2025, and the preservation number of the bacillus subtilis is GDMCC No: 66896. The invention provides bacillus subtilis B-03 with high cellulose and hemicellulose degradation rate, the cellulose degradation rate of the bacillus subtilis B-03 can reach 75.58%, the hemicellulose degradation rate of the bacillus subtilis B-03 is as high as 97.24%, and the cellulose degradation rate and the hemicellulose degradation rate of the bacillus subtilis B-03 are obviously higher than those of existing cellulose bacillus subtilis degrading bacteria; meanwhile, the method can be suitable for a non-sterilization straw fermentation scene and is high in adaptability, so that the method has a wide application prospect in industrial natural environments such as composting and biogas fermentation.

The invention discloses lignin-containing nanocellulose based on camellia oleifera fruit and cattail as well as a preparation method and application thereof, and relates to the technical field of production of biomass nanomaterials, the lignin-containing nanocellulose based on camellia oleifera fruit and cattail comprises the following components in percentage by mass: 8%-15% of lignin, 3%-8% of hemicellulose and 70%-90% of cellulose; the preparation method of the lignin-containing nanocellulose comprises the following steps: S1, treating oil tea fruit cattail; s2, performing DES pretreatment on the Camellia oleifera fruit powder; s3, performing acidolysis and centrifugation on the fruit peel cellulose to obtain lignin-containing nano cellulose; s4, dialyzing the nano cellulose containing the lignin; the lignin-containing nanocellulose is used for preparing the LCNF / PVA composite film, and the LCNF / PVA composite film can be used as a food preservative film; the lignin-containing nanocellulose prepared by the preparation method disclosed by the invention has high specific surface area, high surface chemical activity and good biocompatibility and degradability, and is widely applied to the field of food industry.

The invention belongs to the technical field of lignin, and particularly relates to a method for preparing bamboo lignin with high antioxidant activity through a one-pot two-step method. The method provided by the invention comprises the following four steps: constructing a deep-eutectic solvent, extracting lignin, obtaining a bamboo cellulose-rich substance, and obtaining the degraded bamboo lignin. The eutectic solvent used in the method provided by the invention has the advantages of low vapor pressure, good thermal stability, biodegradability, recyclability and the like, has good environmental friendliness, and conforms to the concept of sustainable development. The bamboo wood lignin is extracted and degraded through a one-pot two-step method, the lignin in the bamboo wood can be efficiently dissolved and separated, meanwhile, damage to cellulose and hemicellulose is small, and the antioxidant activity of the extracted lignin can be remarkably improved. The lignin with high antioxidant activity prepared by the method can be applied to the fields of food, cosmetics, medicines and the like.

The invention provides a method for extracting raspberry ketone through raspberry enzymolysis and ultrasonic wave, and belongs to the technical field of natural product extraction. The method comprises the following steps: selecting cyan raspberry fruits which are about to be ripe and free from diseases and insect pests, washing, freeze-drying and crushing to 30 meshes; then, carrying out composite enzymolysis, and carrying out enzymolysis on cellulase, pectinase and hemicellulase at the mass ratio of 0.8%-1.2% to 3: 1: 2 for 2-4 hours under the conditions that the temperature is 50 DEG C and the pH is 5.0; and extracting for 20-60 minutes by using a 80% ethanol solution under the assistance of the ultrasonic power of 220W and the frequency of 30kHz. And finally, carrying out vacuum concentration and silica gel column chromatography separation to obtain a high-purity product. The method obviously improves the extraction rate, has the advantages of environmental protection and economy, and meets the requirements of industrial production on raspberry ketone extraction.