The invention discloses a
stem cell-based
microvesicle as well as a preparation method and application thereof, belongs to the technical field of
biological tissue engineering, and particularly relates to a preparation method of the
stem cell-based
microvesicle, which comprises the following steps: culturing stem cells until the
cell fusion degree is 80-90%, performing hypoxia treatment, and cleaning for 2-3 times; standing and culturing the cleaned stem cells, then adding a
calcium ion carrier, and carrying out ultrasonic treatment; the serum-free basic culture medium containing the
calcium ion carrier is immediately absorbed and removed, the cells are cleaned through PBS, culture is conducted for 24 h, and
cell supernatant is collected and merged respectively; performing first
centrifugation to remove
cell debris; performing second
centrifugation to remove dead cells and
large cell debris; carrying out third
centrifugation to remove organelles; fourth centrifugation is carried out, large vesicles are removed, and a final supernatant is obtained; and filtering and centrifuging to obtain a
microvesicle precipitate. The preparation method of the
microvesicles aims at systematically improving the yield and
biological activity of the
stem cell microvesicles through low-
oxygen pre-stimulation, ultrasonic-
calcium ion carrier synergistic induction and
differential centrifugation.