20results about How to "Promote differentiation" patented technology

The application discloses a traditional Chinese medicine composition for repairing a wound, a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicines. The traditional Chinese medicine composition has the effects of clearing heat and resolving toxins, eliminating swelling and removing blood stasis, and has the effects of immune regulation and anti-fibrosis, so that the traditional Chinese medicine composition has the effects of dissipating blood stasis and eliminating muscle nodules in the process of repairing the wound. The traditional Chinese medicine composition also has the effects of reducing swelling and relieving pain. The traditional Chinese medicine composition has the effects of stopping bleeding and relieving pain. The traditional Chinese medicine composition has the effects of promoting cell differentiation and promoting muscle regeneration. The traditional Chinese medicine composition has the effects of promoting blood circulation and the traditional Chinese medicine composition has the effects of promoting muscle regeneration. The traditional Chinese medicine composition has the effects of preventing inflammation and scars and promoting wound healing through specific combinations and synergistic effects according to monarchs, ministers, and assistants.

This invention provides a biocompatible β This invention relates to a method for composite modification of titanium alloy surfaces, belonging to the technical field of alloy modification methods. The invention includes applications in medical... β This invention involves introducing bioactive particles onto the surface of a titanium alloy substrate, performing friction stir processing (FSP) on the substrate to introduce bioactive particles and construct a gradient grain structure, and then subjecting the FSP-treated substrate to compressed plasma flow (CPF) treatment to achieve uniform distribution of bioactive particles and construct a topology with an average surface roughness of 3-6 μm suitable for cell growth. The invention precisely controls the amount of bioactive particles introduced and constructs a gradient grain structure through friction stir processing (FSP); simultaneously, compressed plasma flow (CPF) technology achieves uniform distribution of bioactive particles and an ideal surface roughness of 3-6 μm on the titanium alloy surface. Ultimately, this significantly improves the cell adhesion, proliferation capacity, and osteogenic properties of the material surface, solving the technical problem of low integration efficiency of traditional titanium alloy implants with human bone tissue.

The application discloses a construction method of a 3D printing liver organoid support loaded with mesenchymal stem cells assembled by FH1 sodium alginate microspheres, and comprises the following steps: preparing CaO2@ZIF-8@SL; preparing FH1-loaded sodium alginate microspheres FH1@SA; and preparing a 3D printing liver organoid support. The 3D printing liver organoid support prepared by the method can significantly enhance the differentiation of human umbilical cord mesenchymal stem cells into functional stem cells, and can be used for constructing a human liver organoid model.

This invention relates to the field of biopharmaceutical technology, specifically to... SARM1 The application of related reagents in the preparation of products for the diagnosis and / or treatment of hemophagocytic lymphohistiocytosis-like liver injury. The results of this invention demonstrate... SARM1 It is both a circulating biomarker in HLH and an intrinsic regulator of iNKT cell fate. SARM1 By stabilizing LCK and maintaining TCR signaling, excessive iNKT1 bias and IFN-γ-driven liver injury can be prevented. These findings highlight... SARM1 Its important value as a potential diagnostic biomarker and therapeutic target for HLH in children provides a new strategy for the treatment of hemophagocytic lymphohistiocytosis-like liver injury in children.

The invention discloses a co-culture method of cell culture meat and application of the co-culture method in improving meat quality and flavor. According to the invention, through two-stage differentiation, muscle precursor cells and fat precursor cells are respectively subjected to proliferation culture, then are separately subjected to primary differentiation, and are mixed together for co-culture, and meanwhile, second-stage continuous differentiation is carried out; the problem that differentiation of muscle cells and fat cells cannot be effectively and simultaneously differentiated under the co-culture condition due to different requirements of differentiation of muscle cells and fat cells on the serum environment is solved. In addition, the invention also provides an objective standard for evaluating the flavor quality of cell culture meat generated by co-culture. The cell culture meat with different flavors can be obtained by adjusting the proportion of the co-culture cells through the co-culture method, and the flavor quality of the cell culture meat from other sources can be evaluated and detected through the amino acid detection means.

The present application relates to the field of Chinese chestnut planting, and in particular, relates to a Chinese chestnut female-promoting and male-reducing growth regulator as well as a preparation method and application thereof. The Chinese chestnut female-promoting and male-reducing growth regulator comprises the following components: paclobutrazol, 6-benzylaminoadenine and a surfactant; wherein the concentration of the paclobutrazol is 20-100 mg / L; the concentration of the 6-benzylaminoadenine is 20-100 mg / L; and the volume concentration of the surfactant is 1%-3%. The Chinese chestnut female-promoting and male-reducing growth regulator is safe and reliable, has obvious effects, is good in stability and easy to operate, can be applied to the production of chestnut farmers, promotes the increase of female flowers and the reduction of male flowers of Chinese chestnut, promotes the differentiation of female flowers of Chinese chestnut, increases the female-male ratio of Chinese chestnut, and obviously increases the yield of a single Chinese chestnut tree.

The application relates to the technical field of biomedical materials, and specifically discloses a preparation method of a double-crosslinked 3D-printed bone repair scaffold. The scaffold is made of a biphasic calcium phosphate matrix and a gelatin methacrylate hydrogel outer layer. The preparation method comprises the following steps: firstly, preparing biphasic calcium phosphate ink, and constructing a porous scaffold blank through 3D printing; then, carrying out double-crosslinking treatment of calcium chloride solution and glutaraldehyde solution in sequence to obtain a scaffold matrix with enhanced mechanical properties; then, preparing gelatin methacrylate hydrogel loaded with modified teriparatide, and compounding the gelatin methacrylate hydrogel with the scaffold matrix; and finally, obtaining the final product after blue light crosslinking and solidification. The bone repair scaffold has good biocompatibility and osteogenic activity, and can effectively promote bone defect repair. In addition, the preparation method is controllable and has good repeatability, the structure of the scaffold is accurately controlled through the 3D printing technology, and a new approach is provided for the preparation of personalized bone repair materials.

The application relates to the technical field of dental implants, in particular to a trans-zygomatic implant and a processing technology thereof, and the processing technology comprises variable-distance multi-stage sand blasting and acid etching surface treatment on an anchoring part with threads of the trans-zygomatic implant and electrochemical polishing surface treatment on an extension part without threads. The trans-zygomatic implant is treated by variable-distance multi-stage sand blasting combined with acid etching, so that a uniform micron-level rough base and a multi-stage micropore structure can be formed on the titanium surface, osteoblast adhesion and differentiation are facilitated, the early bone combination speed is accelerated, the combination strength is improved, the early implant loosening risk is reduced, the extension part of the trans-zygomatic implant is treated by electrochemical polishing, a smooth surface can be obtained, plaque adhesion and bacterial colonization are greatly reduced, the implant peri-implantitis risk is reduced, and soft tissue adhesion is promoted; the dual optimization of bone anchoring and tissue adhesion is realized, and the long-term function and health of the trans-zygomatic implant under complex stress are guaranteed.

This invention proposes a SNP marker for promoting preadipocyte differentiation in chickens and identifying broilers with high abdominal fat percentage, and its application, belonging to the field of animal molecular genetics. It includes an SNP marker for promoting preadipocyte differentiation in chickens and identifying broilers with high abdominal fat percentage. The SNP marker uses the sequence shown in SEQ ID NO.1 as its starting sequence, with position 51 being either A or G. If the SNP is of the GG genotype, it indicates a broiler with high abdominal fat percentage. Replacing A with G at position 51 promotes preadipocyte differentiation in chickens. It is mainly used to promote preadipocyte differentiation in chickens and identify broilers with high abdominal fat percentage.

This utility model specifically relates to a hydroponic root-controlling tide pot for promoting early flower bud differentiation. The outer pot of this root-controlling tide pot has a placement cavity. A ring-shaped barrier is provided at the bottom of the placement cavity. The outer pot is constructed to support the inner pot using the ring-shaped barrier, and the ring-shaped barrier also separates a water storage cavity at the bottom of the placement cavity. The inner pot is installed inside the placement cavity. A planting cavity is provided in the inner pot. A limiting frame is provided at the bottom of the inner pot. The limiting frame is fitted outside the ring-shaped barrier, providing radial limitation for the inner pot and allowing it rotational freedom around its own axis. Openings are provided on the bottom and side walls of the inner pot. The inner pot is constructed to connect the planting cavity and the placement cavity through the openings, allowing the crop roots in the planting cavity to grow into the placement cavity through the openings, and the rotational movement of the inner pot relative to the outer pot cutting the crop roots. This root-controlling tide pot effectively controls plant root growth, ensures water supply, and improves cultivation quality.

The application discloses a strontium-doped inorganic nanofiber composite 3D printing scaffold for bone defect repair and a preparation method thereof, and belongs to the field of bone defect repair biomaterials. The preparation method comprises the following steps: dissolving strontium nitrate in a mixed solution of tetraethyl orthosilicate, phosphoric acid and deionized water, stirring and mixing the obtained SiO2-SrO sol with a PVA solution, electrospinning, high-temperature calcination, obtaining a SiO2-SrO nanofiber membrane, cutting small pieces, high-speed stirring and dispersing, adding deionized water, high-speed homogenizing, freeze-drying, mixing with deionized water, grinding and freeze-drying, obtaining SiO2-SrO nanofiber as a dispersed phase, magnetically stirring and uniformly mixing CS solution and HA solution as a continuous phase, fully mixing and uniformly mixing to obtain a biological ink, and then 3D printing, freeze-drying and ultraviolet sterilization of the obtained scaffold, and immersion in a crosslinking agent solution for crosslinking reaction. The application realizes macroscopic and microscopic double-layer repair functions of the scaffold by combining electrospinning with 3D printing, and is used for bone defect repair.

This invention discloses a neutral homogeneous polysaccharide from Rehmannia glutinosa, its preparation method, and its applications, belonging to the field of traditional Chinese medicine preparation technology. The polysaccharide is mainly composed of galactose, glucose, and fructose in a molar ratio of 2.72:1.17:1.11, with a weight-average molecular weight of 2152 Da. The neutral polysaccharide from Rehmannia glutinosa can exert a therapeutic effect on osteoporosis by increasing bone mineralization and bone density; it can also promote the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), showing significant application effects in the preparation of stem cell therapy drugs for bone defects. The neutral homogeneous polysaccharide from Rehmannia glutinosa shows great application potential in the preparation of drugs for treating osteoporosis and as a stem cell therapy (especially bone regeneration therapy based on bone marrow mesenchymal stem cells). This provides new candidate substances and research directions for developing efficient and safe treatment strategies for bone metabolic diseases derived from traditional Chinese medicine.

The application discloses knockout poplar ACO gene in the application of improving wood yield and a method thereof, an ACO knockout plant carrier is introduced into NL895 to obtain an ACO knockout transgenic plant; the stem xylem proportion in the knockout plant is significantly higher than that of WT, and the xylem layer number is also significantly increased; the result shows that the poplar stem cambium activity and xylem differentiation are promoted after the ACO is deleted, and the poplar development and utilization have important significance.

This invention belongs to the field of biomedicine and relates to a lipid component for generating Her2-targeting chimeric antigen receptor neutrophils in vivo, its preparation method, and its application in the treatment of solid tumors. The lipid nanoparticles are dual-targeting lipid nanoparticles, with the dual targeting achieved through a Ly6G antibody fragment and a NEBP peptide. The lipid nanoparticles contain a lipid component and mRNA encoding Her2-CAR and IFN-γ. This invention uses lipid nanoparticles (LNPs) to deliver the mRNA encoding Her2-CAR and IFN-γ, delivering it in vivo to neutrophils to express Her2-CAR and IFN-γ, generating CAR-neutrophils. This eliminates the cumbersome steps of in vitro engineering of immune cells and avoids the potential adverse reactions caused by in vitro preparation and reinfusion of CAR-immune cells.

The utility model discloses a kind of self-adaptive creeper frames for honeysuckle planting, including support frame and rotating stand;Support frame quantity is two and is installed in the frame body of two sides of honeysuckle before and after respectively, and the bottom of support frame is fixed in soil layer;Rotating stand quantity is two and is V-shaped distribution between two support frames on two sides, and every rotating stand is square net rack structure and its two sides before and after are respectively rotated and installed on two support frames by pivot;Position corresponding to below every rotating stand between two support frames is fixed with a connecting rod respectively, and the lower end of every rotating stand is connected with multiple elastic members between its corresponding connecting rod.The utility model is by V-shaped layout cooperation dynamic opening and closing, and middle lower part blade light intensity is greatly promoted, and photosynthetic efficiency is doubled, and flower bud differentiation is significantly promoted.Rotating stand dynamic development avoids branch stacking, and potential flower bud visibility is improved, and mispruning rate is reduced.Opening angle increases to strengthen crown layer air circulation, and humidity reduces, and powdery mildew and aphid occurrence rate is greatly reduced.

The application discloses a cultivation method of strontium-rich cordyceps militaris, and relates to the technical field of microbial cultivation, and comprises the following steps: step S1, preparation of a culture medium; step S2, activation of a strain; step S3, liquid seed liquid cultivation; step S4, solid-state cultivation; and step S5, harvesting and drying. The strontium-rich cordyceps militaris cultivated by the cultivation method can realize efficient enrichment of strontium elements, and can guarantee the biomass of the cordyceps militaris and the content of core active ingredients.

This invention discloses a colorectal cancer stem cell sex-inhibiting polypeptide, its expression vector, and its applications, belonging to the field of bioengineering technology. This invention is the first to discover the crucial role of ZCCHC14 protein in maintaining the characteristics of colorectal cancer stem cells, and based on this, develops an inhibitory polypeptide targeting ZCCHC14. Experiments have shown that this polypeptide can significantly downregulate the expression of colorectal cancer stem cell markers, upregulate the expression of differentiation markers, inhibit the spheroidization ability of stem cells, and significantly reduce EdU incorporation rate, effectively weakening the characteristics of colorectal cancer stem cells, promoting cancer cell differentiation, and inhibiting proliferation. It can be applied to the preparation of anti-colorectal cancer drugs, especially drugs that inhibit the proliferation of colorectal cancer cells, and has significant clinical application prospects.

The present application provides a kind of whitening, improve skin barrier composition and its preparation method and application, it is related to skin care product technical field.The composition raw material includes: plant extract 30-42 parts, Lotus nut extract 13-17 parts, Beauveria extract 12-14 parts, malt oligosaccharide glucoside 1.2-1.6 parts, raspberry ketone glucoside 2-3 parts, oligopeptide 4-6 parts, sucrose laurate 1-2 parts, humectant 2-4 parts, thickening agent 1-1.8 parts, water 280-340 parts.The present application can inhibit tyrosinase activity by the synergistic effect of specific plant extract, Lotus nut extract, Beauveria extract, malt oligosaccharide glucoside, raspberry ketone glucoside, oligopeptide and other raw materials, achieve good whitening effect, and make each active ingredient transdermal absorption, protect skin keratin layer lipid bilayer structure from being destroyed, maintain the water-retention capacity of skin, avoid water loss, thereby play the role of improving skin barrier.

This invention relates to the field of agricultural fertilizer technology, and discloses a functional compound fertilizer containing a soil conditioner and its preparation method. The fertilizer provided by this invention is composed of nitrogen, phosphorus, and potassium sources, a compound soil conditioner, microencapsulated functional microbial agents, chelated trace elements, and a slow-release coating agent. The preparation method includes raw material pretreatment, step-by-step mixing, granulation, and coating processes. The fertilizer provided by this invention addresses the shortcomings of existing banana-specific fertilizers, such as poor synergy between soil conditioning and nutrient distribution, mismatch between fertilizer release and growth cycle, and limited stress resistance. It can precisely improve acidic banana plantation soil, achieve dynamic nutrient release, improve fertilizer utilization, reduce the incidence of banana wilt disease, significantly increase yield and fruit quality, is suitable for various cultivation models, and possesses both practical and environmental value.