224results about How to "Promote differentiation" patented technology

The invention provides double-crosslinking acellular matrix hydrogel as well as a preparation method and application thereof, and belongs to the technical field of hydrogel. The preparation method comprises the following steps: (1) carrying out decellularization treatment on a porcine dermal layer to obtain a decellularized porcine dermal matrix, (2) putting freeze-dried powder of the decellularized porcine dermal matrix into an acidic pepsin solution, stirring and digesting, adjusting the concentration, adding 10xPBS, adjusting the pH value to obtain a pregel solution, and carrying out freeze-drying on the pregel solution to obtain the porcine dermal matrix freeze-dried powder. (3) putting gel formed by the pre-gel solution into a tannic acid solution for cross-linking to obtain mono-cross-linked acellular matrix hydrogel; (4) smashing the mono-cross-linked acellular matrix hydrogel, and mixing the smashed mono-cross-linked acellular matrix hydrogel with a glucose oxidase solution and a ferrous ion solution with the concentration of 0.1-10mg / ml to obtain a bi-cross-linked acellular matrix hydrogel precursor; 5, the double-crosslinking acellular matrix hydrogel precursor is placed under the condition of glucose with the concentration being 10-200 mg / dl to form the double-crosslinking acellular matrix hydrogel. The hydrogel prepared through the method can achieve the balance of safety, supporting performance and degradability, and is wide in application range.

The invention provides a culture method for improving immunity of fishes. The culture method comprises the following steps: S1, selection of culture points; S2, specification and layout of net cages; S3, selection and throwing of fish fries; according to the fry disinfection method disclosed by the invention, the fry are soaked and disinfected by using a compound disinfectant prepared from povidone iodine, polysorbate, citric acid and vitamin C in a specific proportion, so that pathogenic microorganisms on the surfaces and gill parts of fish bodies are effectively killed. According to the breeding management, the immunity of the fish body is comprehensively enhanced by feeding the fish with an immune conditioner composed of campesterol palmitate, immunomodulatory peptide, beta-glucan and astaxanthin in a specific proportion. According to the bottom mud management, the content of harmful substances is reduced by putting a bottom mud modifier composed of sodium molybdate, nitrifying bacteria, photosynthetic bacteria, tetrandrine and sodium alginate in a specific proportion, growth of pathogenic bacteria is inhibited, nutrient substance circulation is promoted, the water quality environment is optimized, and healthy growth of fry is promoted.

The invention belongs to the field of biotechnology and medical configuration products, and provides a mucoprotein-tannic acid packaged probiotic preparation as well as a preparation method and application thereof. According to the preparation method disclosed by the invention, tannic acid and mucoprotein are encapsulated in escherichia coli Nissle 1917 and lactobacillus plantarum NC8, so that the mucoprotein-tannic acid encapsulated probiotic preparation is obtained. The probiotic microbial agent prepared by the invention has excellent resistance to severe environment of gastrointestinal tracts, and the adhesiveness of the probiotic microbial agent in intestinal tracts is enhanced, so that the colonization and growth of the probiotics in a mucous layer are enhanced. Meanwhile, bacterial enteritis, especially enteritis caused by enterotoxigenic escherichia coli (ETEC), can be effectively treated.

The invention relates to the technical field of medical instruments, in particular to a multi-layer ultra-light hernia patch which comprises a large-mesh electrostatic spinning tissue ingrowth layer, a mesh woven base material layer and an anti-adhesion layer which are sequentially connected in a stacked mode. Wherein after the large-mesh electrostatic spinning tissue ingrowth layer is implanted into a human body, a cell microenvironment can be better simulated, cell adhesion, differentiation and migration are facilitated, uniform distribution of newborn cells in the fiber direction of an electrospinning membrane is promoted, granulation tissues uniformly ingrow in a defect part, and the electrospinning fiber membrane and the tissues are cooperatively repaired; secondly, the middle layer can provide supporting performance and mechanical performance of the patch in the early stage for the patch, the defects of the electrospinning fiber membrane in the supporting performance and the mechanical performance are overcome, the long-term implantation amount of the patch after later degradation is greatly reduced, and the foreign body sensation is obviously reduced; the anti-adhesion layer also adopts the electrostatic spinning technology, and the anti-adhesion effect is achieved by selecting materials and regulating and controlling the porosity, the aperture and the fiber diameter of the electrospun fiber membrane.

The invention provides a composition with a function of stabilizing skin enzyme activity as well as a preparation method and application of the composition, and the composition is prepared from the following components in parts by mass: 0.001 to 0.01 part of superoxide dismutase, 0.001 to 0.05 part of nicotinamide adenine dinucleotide, 0.11 to 12 parts of a polypeptide compound a and 0.005 to 0.5 part of a polypeptide compound b. The invention also provides a preparation method of the composition and application of the composition in preparation of skin care products for improving skin enzyme activity, repairing skin and resisting aging. The composition can be prepared into emulsion, solution, paste, gel or spray. The composition can effectively improve the enzyme activity of skin, improve the texture and appearance of the skin and repair the skin, and is beneficial to delaying the aging of the skin at the same time.

The invention provides an application of dihydroflavonoid compound naringin in promoting proliferation and differentiation of porcine muscle stem cells to form myotubes, which is characterized in that the naringin is added into a DMEM / F12 culture medium containing 15% of fetal calf serum and 1% of double antibodies and a DMEM differential culture medium containing 2% of horse serum and 1% of double antibodies until the final concentration is 70 mu M, and the porcine muscle stem cells are respectively treated in proliferation and differentiation stages, hoechst 33342 staining and immunofluorescence staining methods are used for respectively judging the number of cells in the proliferation stage and the fluorescence area, fluorescence intensity and nuclear fusion rate of Myosin in myotubes formed in the differentiation stage. The relative proliferation multiple of the muscle stem cells treated by adding naringin and the fluorescence area, the fluorescence intensity and the nuclear fusion rate of Myosin in myotubes are obviously higher than those of a blank control group. By adopting the method, proliferation and differentiation of the porcine muscle stem cells can be promoted, and a new thought is provided for maintaining cell dryness and large-scale production of cultured meat.

The invention discloses a method for promoting diabetic fracture healing by using bone marrow mesenchymal stem cell exosomes, which comprises the following steps: separating MSCs (mesenchymal stem cells) from the bone marrow of a diabetic patient, culturing and extracting the exosomes; the method comprises the following steps: extracting an exosome from a bone fracture part, performing quantitative analysis on the extracted exosome, injecting the exosome into the bone fracture part in a manner of intravenous injection, local injection or minimally invasive injection, continuously treating, promoting proliferation and differentiation of osteoblasts by miRNA or mRNA in the exosome through targeting cells, enhancing angiogenesis, and further accelerating fracture healing. According to the exosome, proliferation and differentiation of osteoblasts are promoted through miRNA or mRNA in the exosome, the synthesis capacity of bone matrixes of the osteoblasts is enhanced, so that fracture healing is accelerated, meanwhile, the exosome provides necessary blood supply for fracture healing by adjusting vascular endothelial cells and promoting angiogenesis, and the repair capacity of fracture parts is effectively improved.

The invention relates to a preparation method of a sodium alginate composite hydrogel microneedle for treating pathological scars, and belongs to the technical field of hydrogel microneedles, the microneedle takes sodium alginate as a main material, the sodium alginate is methylated to obtain methylacryloylated sodium alginate AlgMA, hollow manganese dioxide nanoparticles loaded with matrix metalloproteinase are added, and the microneedle is prepared by a hydrothermal method. A hydrogel microneedle filling solution is obtained; the preparation method comprises the following steps: transferring the hydrogel microneedle into a PDMS microneedle mold, carrying out negative pressure vacuum bubble removal, drying and concentrating twice in a drying oven, carrying out photopolymerization crosslinking, forming a solid hydrogel microneedle tip, adding a 20% PVA solution, and drying in the drying oven to form the microneedle substrate, and the preparation method is simple, convenient and efficient, has industrial application potential, and is suitable for the scenes of drug delivery, skin care and the like; according to the application of the sodium alginate composite hydrogel microneedle in pathological scar drug delivery, the sodium alginate composite hydrogel microneedle has excellent mechanical strength and biocompatibility, and painless and noninvasive drug delivery can be carried out on the surface of scar skin.

The invention relates to the technical field of cutting seedling raising, in particular to a cutting seedling raising method for camellia sinensis. According to the method, the cutting slips are soaked in the mixed solution of IBA, NAA and rooting powder GGR with the specific concentration, rooting of the camellia longissima cutting seedlings can be promoted, and therefore the survival rate of cutting propagation of the camellia longissima is increased. The invention also provides a camellia japonica cutting seedling raising method based on the mixed solution. The camellia japonica cutting seedling raising method comprises the following specific steps: obtaining camellia japonica cuttings and cutting the camellia japonica cuttings into scion sections; soaking the scion sections in a rooting solution to obtain cutting slips; cutting the cutting slips; seedling management. According to the method, a simple, convenient, safe and efficient camellia sinensis cutting seedling raising technical system is established through a cutting means, a theoretical basis can be provided for large-scale production of high-quality camellia sinensis seedlings, and experimental materials are also provided for camellia sinensis tissue culture seedling raising.

The invention relates to the technical field of medical gel, in particular to gynecological vaginal mucosa repairing gel and a preparation method thereof. Comprising the following raw materials: carbomer, glycerol, sodium hyaluronate, vitamin E, potassium sorbate, a modified nano polylactic acid-glycolic acid copolymer, modified nano collagen, albumin, tea tree essential oil, aloe, astragalus membranaceus, dandelion, stigma croci and ginseng. The modified nano polylactic acid-glycolic acid copolymer, the modified nano collagen and the albumin play a role through a synergistic effect, and the modified nano polylactic acid-glycolic acid is used as a skeleton structure for cell growth and is firmly attached to the active pharmaceutical ingredient-albumin compound; the polyacrylic acid chain on the surface controls the drug release rate by sensing the change of the pH value, the albumin initiates endocytosis through specific binding, and the albumin and the modified nano collagen jointly improve the anti-inflammatory performance, promote cell proliferation and differentiation and promote repair and regeneration of vaginal mucosa tissues.

The invention is suitable for the technical field of skin dressings, and provides preparation and application of a polyphenol nano-liposome loaded i-PRF skin dressing, the skin dressing comprises oxidized sodium alginate, carboxymethyl chitosan, freeze-dried concentrated growth factors and oleuropein nano-liposomes, the concentration of the oxidized sodium alginate is 0.2-0.5 g / ml, the concentration of the carboxymethyl chitosan is 0.2-0.5 g / ml, the concentration of the freeze-dried concentrated growth factors is 0.2-0.5 g / ml, and the concentration of the oleuropein nano-liposomes is 0.2-0.5 g / ml. The concentration of the carboxymethyl chitosan is 0.2 to 0.5 g / ml, the concentration of the freeze-dried concentrated growth factor is 0.05 to 0.1 g / ml, and the concentration of the oleuropein nano-liposome is 0.125 to 1 ml / g. According to the skin dressing, the biocompatibility of the hydrogel is effectively improved, the sustained drug sustained release characteristic is shown, and the skin dressing has outstanding performance in the aspect of promoting wound healing.

The invention discloses a method for designing a bionic bone scaffold based on wall thickness mixed gradient change, and belongs to the technical field of bionic bone scaffolds. By designing the wall thickness mixed gradient function, the lattice unit can have wall thickness gradient change in the radial direction and the axial direction at the same time, so that the porosity also has gradient change in three directions at the same time, and the defect that a bone scaffold is designed by using a lattice material (beam unit) unit and a TPMS unit in the prior art can be well overcome; and the stent better conforms to the natural bone structure and mechanical property requirements. The designed bionic bone scaffold is excellent in mechanical property, high in compression resistance and deformation resistance and capable of promoting cell growth and tissue regeneration.

The invention belongs to the technical field of cell culture, and particularly relates to a method for promoting proliferation and differentiation of cervical squamous epithelial cells. The method comprises the following steps: S1, extracting and culturing primary cervix uteri squamous epithelial cells from cervix uteri tissues to obtain primary cervix uteri squamous epithelial cells; s2, adding the primary cervical squamous epithelial cells into a cell proliferation induction culture solution to induce cell proliferation, so as to obtain proliferated cervical squamous epithelial cells; and S3, uniformly mixing the proliferated cervical squamous epithelial cells with differentiation matrigel, and inducing cell differentiation by using a cell differentiation induction culture solution to obtain differentiated cervical squamous epithelial cells. Proliferation and differentiation of the cervical squamous epithelial cells are promoted by preparing a proper culture medium, and influence factors of proliferation and differentiation of the cervical squamous epithelial cells are researched by culturing the cervical squamous epithelial cells in vitro, so that the pathogenesis of cervical erosion and cervical cancer can be clarified; therefore, a direction is provided for research and development of medicines for treating cervical erosion and cervical cancer.

The invention relates to the technical field of collagen dressings, in particular to a moisturizing and repairing collagen dressing and a preparation method thereof. The moisturizing and repairing collagen dressing is prepared from the following components in parts by mass: 10 to 30 parts of recombinant human fibronectin, 10 to 20 parts of recombinant collagen, 5 to 15 parts of beta-glucan, 5 to 10 parts of compound hexanediol, 40 to 50 parts of carbomer, 5 to 15 parts of a flavoring agent, 80 to 120 parts of purified water, 10 to 30 parts of a modified recombinant human III type collagen solution and 10 to 20 parts of a modified chitosan solution. The recombinant human fibronectin disclosed by the invention can be deposited at an injured part to form a blood clot, so that the aim of stopping bleeding is fulfilled, and the subcutaneous tissue is protected; the recombinant collagen has high biological activity and can inhibit inflammation and improve the conditions of pachulosis, damage and the like of the skin; the beta-glucan can generate cytokines, promote healing of skin with laser-dependent dermatitis or barrier damage, and realize anti-allergic and anti-inflammatory effects.

The invention relates to an oncolytic virus VSV-aPDL1 / aCD137 for expressing a bispecific antibody as well as a construction method and application of the oncolytic virus VSV-aPDL1 / aCD137. The preparation method comprises the following steps: firstly designing aPDL1 / aCD137, then synthesizing an aPDL1 / aCD137 gene in vitro, amplifying the aPDL1 / aCD137 gene, connecting the aPDL1 / aCD137 gene to a pT7-VSV whole genome plasmid to obtain a virus whole genome plasmid pT7-VSV-aPDL1 / aCD137, and then co-transfecting the plasmid and a helper plasmid to a 293T cell to obtain the VSV-aPDL1 / aCD137 oncolytic virus. According to the present invention, the local anti-tumor immune response of the tumor is activated through the virus, the infiltration of the lymphocyte to the tumor tissue is recruited, the anti-PD-L1 / anti-CD137 bispecific antibody is expressed, the inhibition of the PD-1 / PD-L1 pathway on the T cell is blocked, and the inhibition signal of the PD-1 / PD-L1 pathway on the tumor cell surface is reversed into the CD137 signal of the activated T cell, such that the functional failure of the T cell caused by the PD-1 / PD-L1 pathway can be blocked, and the anti-tumor immune response of the tumor can be inhibited; the function and viability of T cells can be further improved, long-term immune surveillance on tumors is realized, and long-acting anti-tumor immune response is generated. Experiments show that the oncolytic virus can significantly prolong the lifetime of tumor-bearing mice, and a new strategy is provided for development of solid tumor drugs.

The invention discloses injectable and degradable composite drug-loading hydrogel as well as a preparation method and application thereof. The injectable and degradable composite drug-loading hydrogel comprises sodium alginate hydrogel, piezoelectric nanofibers and triamcinolone acetonide and / or ropivacaine, wherein the piezoelectric nanofibers and the triamcinolone acetonide and / or the ropivacaine are loaded on the sodium alginate hydrogel; the piezoelectric nanofibers are poly-L-lactic acid nanofibers. Through the synergistic effect of controllable release of the medicine and piezoelectric stimulation, the dual effects of continuous analgesia and neural restoration are achieved in the PHN treatment process, more effective treatment selection is provided for a PHN patient, complications caused by a traditional implantable electrode can be avoided, a more minimally invasive, convenient and safe treatment mode can be provided for the patient, and the treatment effect is good. And a theoretical basis is provided for future clinical practice.

The invention discloses a cell sheet taking an acellular biological membrane as a carrier and having osteogenic activity as well as a preparation method and application of the cell sheet. The method comprises the following steps: firstly, carrying out decellularization treatment on a natural amnion, and then planting target cells on a biological membrane; and culturing the preliminarily constructed biological membrane to obtain the cell sheet taking the decellularized biological membrane as the carrier. The cell sheet prepared by the invention has excellent capability of promoting cell proliferation and osteogenic differentiation, so that tissue repair is promoted. The prepared cell sheet overcomes the defect that existing cell transplantation carriers generally lack activity, the preparation process is simple and controllable, and breakthrough of stem cell therapy in tissue engineering is facilitated.

The invention discloses an apple rootstock tissue culture seedling raising method, and relates to the technical field of tissue culture. The method comprises the following steps: (1) washing annual robust branches with tender shoots in a dormancy period with running water, soaking with soapy water, cleaning, obliquely shearing into stem segments, soaking with alcohol, washing, sterilizing, washing, absorbing moisture, and shearing off wounds to obtain pretreated explants; (2) inoculating the pretreated explant into a differential culture medium, and culturing to obtain an adventitious bud; (3) transferring the adventitious buds into a proliferation culture medium, and performing dark culture and light culture to obtain adventitious bud clusters; (4) inoculating the adventitious bud clusters into a rooting culture medium, and culturing to obtain tissue culture seedlings; and (5) hardening the tissue culture seedlings, washing off the culture medium, disinfecting, putting into a culture medium, culturing to obtain apple seedlings, and transplanting to outdoor soil. According to the method, the seedlings capable of being transplanted outdoors can be rapidly obtained, and the problems of infectious microbe pollution, low survival rate, poor rooting effect and the like in existing tissue culture seedling culture are effectively solved.

The present invention discloses a recombinant collagen and its use in cartilage repair matrix. The recombinant collagen provided by the present invention comprises a sequence shown in SEQ ID No.1; the amino acid sequence of the recombinant collagen comprises N basic repeating units, and the basic repeating unit contains n1 of the following characteristic amino acid sequences: "G‑Xaa1‑Xaa2‑G‑E‑Xaa3"; the 3' end and the 5' end of the basic repeating unit are connected to form the above-mentioned characteristic amino acid sequence. The recombinant collagen provided by the present invention has a relatively obvious integrin binding activity, has the effects of promoting cell adhesion, proliferation, differentiation, repairing cartilage tissue defects, etc., and has good application prospects.

The composition for repairing the meniscus comprises a growth differentiation factor and emodin, and is used for improving inflammation and oxidative stress in a microenvironment and promoting a cell chondrogenic differentiation process. Through verification, according to the core-shell co-delivery nano-carrier provided by the invention, two bioactive compounds, namely emodin and a growth differentiation factor, are wrapped by mesoporous silicon dioxide nano-particles, and the two bioactive compounds are used for promoting fibrocartilage differentiation. The double-drug nano delivery system provides continuous release of emodin and GDF, synergistically regulates severe inflammation and oxidation environments, and promotes in-vitro and in-vivo fibrous cartilage regeneration. Benefited from this, according to the invention, a three-dimensionally printed meniscus-shaped PCL framework is combined with the composition by using cartilage-specific matrix hydrogel, a stability-enhanced meniscus replacement model is constructed, a cartilage immune microenvironment is synergistically adjusted, and successful repair of animal (rabbit) full meniscus replacement is realized.

The invention provides a biological vascular stent material and a preparation method thereof, and belongs to the technical field of biomedical materials. The polylactic acid-polyvinyl alcohol emulsion is used as a skin layer spinning solution, a sodium alginate solution containing exosomes is used as a core layer spinning solution, a composite material is prepared through a coaxial electrostatic spinning technology, and the biological vascular stent material is obtained in cooperation with a post-treatment process, so that the porosity of the material is effectively improved, and the biological vascular stent is prepared. Meanwhile, the elongation and the supporting force of the intravascular stent are increased.

The invention discloses an apple rootstock tissue culture seedling raising method, and relates to the technical field of tissue culture. The method comprises the following steps: (1) washing annual robust branches with tender shoots in a dormancy period with running water, soaking with soapy water, cleaning, obliquely shearing into stem segments, soaking with alcohol, washing, sterilizing, washing, absorbing moisture, and shearing off wounds to obtain pretreated explants; (2) inoculating the pretreated explant into a differential culture medium, and culturing to obtain an adventitious bud; (3) transferring the adventitious buds into a proliferation culture medium, and performing dark culture and light culture to obtain adventitious bud clusters; (4) inoculating the adventitious bud clusters into a rooting culture medium, and culturing to obtain tissue culture seedlings; and (5) hardening the tissue culture seedlings, washing off the culture medium, disinfecting, putting into a culture medium, culturing to obtain apple seedlings, and transplanting to outdoor soil. According to the method, the seedlings capable of being transplanted outdoors can be rapidly obtained, and the problems of infectious microbe pollution, low survival rate, poor rooting effect and the like in existing tissue culture seedling culture are effectively solved.

The invention discloses a liquid fertilizer formula prepared by compounding a tylosin finished product liquid and other components, and relates to the technical field of fertilizer preparation, and the liquid fertilizer formula comprises the steps of special pretreatment of tylosin, auxiliary compounding, nutrient substance addition and quality detection post-treatment. Certain carbon-carbon bonds, carbon-hydrogen bonds and other chemical bonds in tylosin molecules and byproducts thereof can be broken and recombined under bombardment of active particles, more functional groups with high activity are generated, the biological activity of the finished tylosin liquid can be enhanced, the finished tylosin liquid can be more easily compounded with other components in the subsequent process, and the stability of the finished tylosin liquid is improved. A stable compound with better synergy is formed by means of chemical bonding, hydrogen bonding and the like, ultrasonic waves are uniformly spread in a supercritical carbon dioxide medium, generated high-frequency vibration, cavitation effect and the like act on all the components, and the agglomeration phenomenon possibly existing among the components is further broken.

The invention provides hypoxia regulation exosome hydrogel and a preparation method and application thereof in bone defect repair, and compared with the prior art, the hypoxia regulation exosome hydrogel provided by the invention has the advantages that the exosome cultured by 3D stem cells modified by superparamagnetic ferroferric oxide nanoparticles is loaded on the GelMA hydrogel modified by bone targeting polypeptide, and the preparation method is simple. The intelligent oxygen-producing gel is innovatively constructed by skillfully utilizing the relationship between hypoxia and ROS generation according to the challenge that the hypoxia microenvironment of a bone defect part is closely followed. According to the hydrogel disclosed by the invention, osteogenesis regeneration is promoted by regulating and controlling a Wnt / beta-catenin pathway in osteoblasts, so that hypoxia and oxidative stress microenvironments are safely, efficiently and intelligently improved at injured parts. And the adhesion to the defect part is improved, and the problem that the superparamagnetic ferroferric oxide nanoparticles are difficult to effectively retain at the defect part is solved.

The invention discloses a method for shortening a grape breeding fruiting period, which comprises the following steps: S1, sowing: accelerating germination and sowing hybrid seeds subjected to low-temperature stratification treatment in the first ten days of February to obtain seedlings; s2, transplanting: in the middle ten days of March, when the seedlings obtained in the S1 grow 5-6 true leaves, transplanting the seedlings into a ridged greenhouse nursery garden; s3, primary grafting: in the last ten days of April, selecting semi-lignified branches with the thickness of more than 0.4 cm and full bud eyes of hybrid seedlings, and grafting the semi-lignified branches to current-year semi-lignified new tips of the cutting seedlings of the rootstock of the Zhichang anti-rootstock No.1; s4, secondary grafting: in the last ten days of May, when the young sprouts of the hybrid seedlings after primary grafting grow to 30-50cm and the thickness is 0.4 cm or more, buds on the semi-lignified young sprouts are adopted for secondary grafting. The method is carried out in a greenhouse, current-year grafting and current-year forming can be achieved by adopting a high-position grafting flat shed erecting potential, the child process is manually intervened, the trunk growth time is skipped, and flowering and fruiting as soon as possible are promoted by utilizing the powerful root system of the Zhichang anti-anvil No.1.

The present invention discloses a fibrinogen-based patch, a preparation method and application thereof, wherein the patch comprises fibrinogen and a stabilizer, wherein some lysine residues of the fibrinogen contain hydrophobic groups; and the stabilizer is used to maintain the activity of the fibrinogen. The preparation method comprises: adding a hydrophobic modification reagent dropwise to a fibrinogen solution, performing dialysis after the reaction, and then freeze-drying to obtain the patch. The patch prepared by the method has excellent mechanical strength and adhesion properties, and its performance is significantly better than that of existing surgical adhesive products and emergency hemostasis products under the same conditions. The patch has good biocompatibility, can be absorbed by the human body, and has great potential in clinical applications.

[Problems] To provide a method for producing regenerated T cells via iPS cells into which TCRs isolated from tumor tissue-infiltrating CD106-positive T cells have been introduced. [Solutions] Provided is a method for producing regenerated T cells via iPS cells, comprising: 1. A step for preparing cDNAs respectively encoding a TCR α chain and a TCR β chain from individual cells in a CD106-positive T cell population obtained from subjects and having reactivity with a tumor-related antigen; 2. A step for reprogramming peripheral blood mononuclear cells which are obtained from the subjects and from which B cells and T cells have been removed or T cells into iPS cells, and selecting iPS cell clones having high efficiency of differentiation into T cells from the obtained iPS cells; 3. A step for introducing the cDNAs into the iPS cell clones, hematopoietic stem cells differentiated from the iPS cell clones, immature T cells differentiated from the hematopoietic stem cells, or mature T cells differentiated from the immature T cells; and 4. A step for performing the differentiation of the iPS cell clones having the cDNAs introduced therein, the hematopoietic stem cells or the immature T cells which have been obtained in step 3 into mature T cells and the proliferation of the mature T cells.

The invention provides a mesenchymal stem cell composition as well as a preparation method and application thereof in knee joint products, and belongs to the technical field of medicines. The invention relates to application of a mesenchymal stem cell combined naringin derivative in preparation of medicines for treating osteoporosis and repairing knee joint injury and arthritis. The naringin derivative is prepared from naringin through an ether esterification reaction, the metabolic cycle of the naringin derivative is prolonged and the water solubility is improved while the original activity is maintained, so that the free drug concentration and tissue infiltration capacity in bones are improved, the bioavailability of the naringin derivative is improved, and the bioavailability of the naringin derivative is improved. The prepared mesenchymal stem cell composition can induce mesenchymal stem cell osteogenic differentiation and improve bone repair capacity, has a good intervention effect on osteoporosis, has a sustained and controlled release effect and prolongs medicinal time.

The present invention relates to the use of plumbagin in the preparation of a drug for combating sarcopenia. Experiments have shown that plumbagin can improve the muscle mass and strength of sarcopenia model mice, improve functional disorders, and promote myoblast differentiation and myotube fusion, which provides a new drug for the treatment of sarcopenia.

The invention discloses a method for designing a bionic bone scaffold based on unit cell size gradient change, and belongs to the field of biological material design. The method comprises the following steps: calculating the overall wall thickness of FCC, TH and G cell size gradient models according to the required porosity; a unit is selected as a unit cell size radial gradient, in order to better simulate a natural bone structure, the overall porous structure morphology is designed as a cylinder model, and the unit cell sizes of the outer layer and the inner layer of each model are designed as required; the overall porosity of the three novel porous structure scaffolds reaches 55% by changing the wall thickness; carrying out mechanical property testing on the novel porous structure; observing the compression deformation behavior of each model and recording the numerical results of the elastic modulus and the yield strength after simulation; the designed novel porous scaffold has different functional gradients in the radial direction, and the application range of the energy absorption material unit in bionic bone scaffold design is expanded.