16 results about "Neural differentiation" patented technology

The invention relates to the technical field of nerve cell differentiation, and discloses a culture medium for promoting neural differentiation of SH-SY5Y cells. The culture medium disclosed by the invention is prepared from the following components: tazarotene (tazarotene) and a basic culture medium. The culture medium can promote SH-SY5Y cells to present neuron-like differentiation within 5 days, significantly improves the length of neurite and the expression level of neuron markers, and is low in cytotoxicity. The invention provides an efficient, safe and standardized new tool for neural disease in-vitro model construction, drug screening and mechanism research.

The invention relates to the medical field of urinary surgery, in particular to a fault diagnosis software system of an intelligent urinary surgery catheterization device, which is characterized in that an original data stream is collected by a unified timestamp, and normalized data is obtained through cross-brand semantic adaptation; utilizing inertial measurement exogenous input to suppress body motion artifacts and generating channel credibility; forming structured features based on second-order scattering and cross-channel attention; outputting pressure and flow velocity prediction under a physically constrained reversible and neural differential hybrid model, and inverting potential physical parameters; uncertainty calibration is carried out in combination with a brand-level calibration set, and a fault occurrence probability and a predicted failure time interval are given; a channel fault can be diagnosed and identified in cooperation with self-inspection and fault separation, and sound-light alarm and cross-terminal routing are achieved through arrangement of a system in a hospital. The system is suitable for bedside continuous monitoring and multi-brand deployment, and realizes robust diagnosis and short-term prediction under unified semantics.

The invention provides a whole-brain organoid based on human induced pluripotent stem cells as well as a construction method and application thereof, and belongs to the technical field of organoid construction. The method comprises the following steps: culturing human induced pluripotent stem cells in a complete culture medium to obtain a cell aggregate; culturing in an embryoid body forming culture medium to obtain a spherical embryoid body; culturing in a nerve induction culture medium tissue to form a nerve epithelium structure; embedding the single embryoid body with the nerve epithelium structure in a Matrigel gel drop, culturing in a nerve expansion culture medium to form a nerve epithelium-like structure, and replacing a mature culture medium for culturing to obtain the whole-brain organ. According to the method, by dynamically and accurately regulating and controlling culture parameters, the control ability of a cell differentiation area is improved, and interference of non-target cell types is reduced, so that the efficiency and consistency of embryoid formation and neural differentiation are directly improved, and finally, the obtained mature organoid has a more complete epithelial structure and cell polarity.

The application belongs to the field of stem cell biology and relates to a culture method of a 3D nerve immune organoid containing microglia cells, which comprises the following steps: S1, inducing induced pluripotent stem cells in ectoderm direction and mesoderm direction respectively to obtain nerve-like embryoid bodies and yolk sac-like embryoid bodies; S2, culturing the nerve-like embryoid bodies to make them differentiate into nerves to obtain nerve ring structures, re-digesting the nerve ring structures to obtain nerve progenitor cell single cells, and culturing the yolk sac-like embryoid bodies to make them generate macrophage progenitor cells; and S3, fusing the nerve progenitor cell single cells and the macrophage progenitor cells and continuing to culture to form the 3D nerve immune organoid containing microglia cells. The brain-like organoid of the application can realize the following functions: exploring the control of microglia cells on the proportion of progenitor cells in the development stage, the change of phagocytosis, the control on the number of mature neurons, the influence on cell proliferation and apoptosis, and simultaneously accepting external stimulation and making corresponding functional stress changes.

The technical problem addressed by the present invention is to provide a method for producing an apparent cross-karyocyte-like organ. This technical problem is solved by a method for producing a cross-seeded organ, said method comprising: (1) a step for culturing pluripotent stem cells in an intermediate medium between an undifferentiated maintenance medium and a neural differentiation medium; (2) a step for culturing the cells obtained in step (1) in a neural differentiation medium; and (3) a step for forming a cell mass prior to the start of step (2): (A) 6000 or more raw material cells are inoculated to form one cell mass, and (B) when the start of step (2) is set to the 0th day, the culture medium contains a Sonic Hedgehog signaling pathway acting substance from the 3rd day, and when the start of step (2) is set to the 0th day, the culture medium contains the Sonic Hedgehog signaling pathway acting substance from the 3rd day.

The invention discloses a neural network permanent magnet synchronous motor control method based on neural differentiation and a backstepping strategy, and belongs to the field of motor control, and the method comprises the steps: obtaining the sensing information of a permanent magnet synchronous motor, predicting the sensing information through a neural differentiation framework, and obtaining the nonlinear composite interference of a motor system; wherein the neural differential framework is a model combining a differential equation system and a neural network; according to the sensing information, a controller is constructed through a backstepping strategy, the input of the controller is an auxiliary variable and nonlinear composite interference, and the output of the controller is motor q-axis current data; and calculating the sensing information and the nonlinear composite interference through a controller to obtain neural network permanent magnet synchronous motor control information. According to the invention, the intellectualization and adaptive capability of the motor system under complex working conditions are further improved.

The invention provides inter alia a method for the production of an adherent cortical organoid, comprising the steps of: (a) providing a cell culture comprising a population of neural progenitor cells (NPCs); (b) seeding neural progenitor cells (NPCs) of said population in a well of a multiwell plate; wherein said well comprises a bottom surface (106) and an upright wall (104), and wherein a distance (x) between a centre (112) of the bottom surface and the upright wall is between 1.1 mm and 1.9 mm; and (c) culturing said seeded NPCs in a culture medium for neural differentiation for a period of time, to thereby allow formation of an adherent cortical organoid.

The present invention discloses a method for promoting neural differentiation of mesenchymal stem cells by regulating target genes through exosomal miRNA, and belongs to the field of biotechnology. The method for promoting neural differentiation of mesenchymal stem cells by regulating target genes through exosomal miRNA includes the following steps: S1: extracting mesenchymal stem cells; S2: freezing and storing the obtained mesenchymal stem cells; S3: taking out the non-destructive sampling cryopreservation tube; S4: culturing with MSC culture medium until the culture dish is covered; S5: isolating mononuclear cells; S6: obtaining neural stem cell-like cells; S7: obtaining differentiated neural cells. The present invention solves the problem that the mesenchymal stem cells cultured in the prior art will reduce the immunoregulatory ability of mesenchymal stem cells and affect their application in the treatment of immune-related diseases. The present invention can promote cell proliferation and differentiation and improve the immunoregulatory ability of mesenchymal stem cells.

The application belongs to the field of biomedical science and neurology, and particularly relates to application of CHCHD2 gene and a protein coded by the CHCHD2 gene in preparation of a product for promoting proliferation and differentiation of neural stem cells. The application proves that the CHCHD2 gene and the protein coded by the CHCHD2 gene are highly expressed in in-vivo neural development and in-vitro neural differentiation process; and that knocking down or over-expressing the CHCHD2 gene or the protein coded by the CHCHD2 gene has a certain regulating effect on proliferation and differentiation of neural stem cells. The application provides a new scheme for preparation of a product for promoting proliferation and differentiation of neural stem cells, and provides a new target for drug research and development of neural regeneration and repair treatment of central nervous system development deficiency diseases, neurodegenerative diseases, cerebrovascular diseases, craniocerebral and spinal cord injuries.

The invention discloses an Alzheimer's disease risk gene defect nerve cell as well as a preparation method and application thereof. The engineered induced pluripotent stem cell comprises an induced pluripotent stem cell which is deficient in ABCA7 gene and expresses a neural differentiation transcription factor in an inducible manner. According to the present invention, the Alzheimer's disease (AD) risk gene defect is introduced into the existing pluripotent stem cell line through the CRISPR and other gene editing means, and the inducible expression neural differentiation transcription factor is introduced through the lentiviral vector so as to establish the engineered AD risk gene defect pluripotent stem cell line, the engineered AD risky gene defect pluripotent stem cell line can be used for rapidly preparing nerve cells with Alzheimer's disease related A beta pathological phenotypes in a large scale by adding antibiotics for induced differentiation and screening culture, and is used for researching AD related pathogenesis and intervention means.

The present invention discloses the use of purple sweet potato pigment in the preparation of an induced stem cell neural differentiation product, which belongs to the field of biomedicine technology. The stem cell is a mesenchymal stem cell. The mesenchymal stem cell is a bone marrow mesenchymal stem cell. The neural differentiation is differentiation into acetylcholine neurons. The induced stem cell neural differentiation product includes a drug that induces stem cells to differentiate into neurons or a culture medium that induces stem cells to differentiate into neurons. The induction method is: pretreating stem cells with a growth factor mixture, and then adding purple sweet potato pigment to promote the differentiation of stem cells into neurons. The concentration of the purple sweet potato pigment is 50~100μg / mL. Through research, the present invention found that purple sweet potato anthocyanins can increase miR-223-3p, target Atg2b, inhibit the development of autophagy, and then induce BMSCs to differentiate into cholinergic neurons.

The invention provides colostrum derived stem cell populations, compositions and supplements having antimicrobial properties for enhancing mammalian health and for treating or preventing microbial contamination or infection. Also provided are methods of producing these populations, compositions and supplements from a female mammal in an inflammatory state. Also provided are colostrum derived stem cell populations and method of inducing differentiation into neural cells in neural progenitor medium including mammalian cell culture medium supplemented with casein depleted fraction of whey derived from colostrum. Also provided are neural progenitor medium, population of in vitro differentiated neural cells and pharmaceutical compositions containing same for use in treating neurological disease or disorder.

The present invention discloses a multi-channel cell-loaded nerve repair catheter with a double-patterned structure and a preparation method thereof. The preparation method comprises: ① preparing conductive fibers by wet spinning and in-situ surface polymerization to form conductive fiber bundles; ② designing a casting mold; ③ coating a release agent on the inside of the mold, and fixing the prepared fiber bundle in the mold, adding a UV-curable polymer solution to the mold, and curing it into a gel; ④ after the gel is aged, demoulding; after removing the surface release agent, using an immersion method to introduce a protein / polypeptide material that can promote cell adhesion on the surface of the nerve catheter, drying and sterilizing to obtain a multi-channel nerve repair catheter material with a double-patterned structure; combining the co-culture technology of neural stem cells, after the cells adhere and migrate in a directional manner in the tube, combining the pulsed direct current stimulation technology to achieve neural differentiation, and it can be used to replace autologous nerve transplant materials. The catheter material can be used in the fields of nerve and spinal cord repair.

The present invention discloses a multi-layered cell-loaded nerve repair conduit with the functions of conducting electricity, resisting inflammation and promoting nerve differentiation, and a preparation method thereof. The preparation method is as follows: 1) preparing PLGA fibers doped with graphene, weaving them to obtain a layer of cylindrical fiber mesh; 2) preparing a HAMA mixed solution mixed with microspheres loaded with nerve growth factor and manganese dioxide nanoparticles, and a HAMA solution loaded with neural stem cells; 3) fixing the cylindrical fiber mesh in a cylindrical groove mold, pouring the HAMA mixed solution, and freeze-drying after UV curing to obtain a two-layer porous composite scaffold; 4) immersing it in the HAMA solution loaded with neural stem cells, rapidly curing to form a three-layer cell-loaded gel layer, and culturing in vitro for 4-8 weeks to obtain a multi-layered cell-loaded nerve repair conduit. The nerve repair conduit has the functions of conducting electricity, resisting inflammation and promoting nerve differentiation, and provides a new strategy for the repair of nerve and spinal cord injuries.

The invention belongs to the field of stem cell biology, and relates to a culture method of a 3D neural immune organoid containing microglial cells, which comprises the following steps: S1, respectively inducing induced pluripotent stem cells in an ectodermal direction and a mesodermal direction to obtain a neural embryoid and a yolk sac embryoid; s2, culturing the neural embryoid to perform neural differentiation to obtain a neural garland structure, and re-digesting the neural garland structure to obtain a neural progenitor cell single cell; culturing the yolk sac embryoid to enable the yolk sac embryoid to generate macrophage progenitor cells; and S3, fusing the neural progenitor cell single cell and the macrophage progenitor cell, and continuously culturing to form the 3D neural immune organoid containing the microglial cells. The brain-like organ provided by the invention has the functions of exploring the control of microglial cells on the progenitor cell proportion, the change of phagocytic function, the control on the number of mature neurons and the influence on cell proliferation and apoptosis in the development stage, and meanwhile, the brain-like organ can accept external stimulation and make stress change of corresponding functions.

The present invention relates to a bioactive polypeptide synthesized on the basis of histone demethylase, and the use thereof in the neural differentiation process of mesenchymal stem cells. Disclosed in the present invention are possible protein-protein interaction binding sites of histone demethylase KDM2B and histone methylate EZH2, and disclosed are the roles of KDM2B and the bioactive polypeptide synthesized on the basis of the same in the neural differentiation process of mesenchymal stem cells and the tissue regeneration of injured spinal nerves. On this basis, it is concluded that KDM2B and the bioactive polypeptide synthesized on the basis of the same may play a role in promoting the neural differentiation of stem cells from apical papilla and the regeneration of injured spinal nerves.