6 results about "Neural differentiation" patented technology

The invention relates to the technical field of nerve cell differentiation, and discloses a culture medium for promoting neural differentiation of SH-SY5Y cells. The culture medium disclosed by the invention is prepared from the following components: tazarotene (tazarotene) and a basic culture medium. The culture medium can promote SH-SY5Y cells to present neuron-like differentiation within 5 days, significantly improves the length of neurite and the expression level of neuron markers, and is low in cytotoxicity. The invention provides an efficient, safe and standardized new tool for neural disease in-vitro model construction, drug screening and mechanism research.

The invention relates to the medical field of urinary surgery, in particular to a fault diagnosis software system of an intelligent urinary surgery catheterization device, which is characterized in that an original data stream is collected by a unified timestamp, and normalized data is obtained through cross-brand semantic adaptation; utilizing inertial measurement exogenous input to suppress body motion artifacts and generating channel credibility; forming structured features based on second-order scattering and cross-channel attention; outputting pressure and flow velocity prediction under a physically constrained reversible and neural differential hybrid model, and inverting potential physical parameters; uncertainty calibration is carried out in combination with a brand-level calibration set, and a fault occurrence probability and a predicted failure time interval are given; a channel fault can be diagnosed and identified in cooperation with self-inspection and fault separation, and sound-light alarm and cross-terminal routing are achieved through arrangement of a system in a hospital. The system is suitable for bedside continuous monitoring and multi-brand deployment, and realizes robust diagnosis and short-term prediction under unified semantics.

The invention provides a whole-brain organoid based on human induced pluripotent stem cells as well as a construction method and application thereof, and belongs to the technical field of organoid construction. The method comprises the following steps: culturing human induced pluripotent stem cells in a complete culture medium to obtain a cell aggregate; culturing in an embryoid body forming culture medium to obtain a spherical embryoid body; culturing in a nerve induction culture medium tissue to form a nerve epithelium structure; embedding the single embryoid body with the nerve epithelium structure in a Matrigel gel drop, culturing in a nerve expansion culture medium to form a nerve epithelium-like structure, and replacing a mature culture medium for culturing to obtain the whole-brain organ. According to the method, by dynamically and accurately regulating and controlling culture parameters, the control ability of a cell differentiation area is improved, and interference of non-target cell types is reduced, so that the efficiency and consistency of embryoid formation and neural differentiation are directly improved, and finally, the obtained mature organoid has a more complete epithelial structure and cell polarity.

The application belongs to the field of stem cell biology and relates to a culture method of a 3D nerve immune organoid containing microglia cells, which comprises the following steps: S1, inducing induced pluripotent stem cells in ectoderm direction and mesoderm direction respectively to obtain nerve-like embryoid bodies and yolk sac-like embryoid bodies; S2, culturing the nerve-like embryoid bodies to make them differentiate into nerves to obtain nerve ring structures, re-digesting the nerve ring structures to obtain nerve progenitor cell single cells, and culturing the yolk sac-like embryoid bodies to make them generate macrophage progenitor cells; and S3, fusing the nerve progenitor cell single cells and the macrophage progenitor cells and continuing to culture to form the 3D nerve immune organoid containing microglia cells. The brain-like organoid of the application can realize the following functions: exploring the control of microglia cells on the proportion of progenitor cells in the development stage, the change of phagocytosis, the control on the number of mature neurons, the influence on cell proliferation and apoptosis, and simultaneously accepting external stimulation and making corresponding functional stress changes.

The technical problem addressed by the present invention is to provide a method for producing an apparent cross-karyocyte-like organ. This technical problem is solved by a method for producing a cross-seeded organ, said method comprising: (1) a step for culturing pluripotent stem cells in an intermediate medium between an undifferentiated maintenance medium and a neural differentiation medium; (2) a step for culturing the cells obtained in step (1) in a neural differentiation medium; and (3) a step for forming a cell mass prior to the start of step (2): (A) 6000 or more raw material cells are inoculated to form one cell mass, and (B) when the start of step (2) is set to the 0th day, the culture medium contains a Sonic Hedgehog signaling pathway acting substance from the 3rd day, and when the start of step (2) is set to the 0th day, the culture medium contains the Sonic Hedgehog signaling pathway acting substance from the 3rd day.

The invention belongs to the field of stem cell biology, and relates to a culture method of a 3D neural immune organoid containing microglial cells, which comprises the following steps: S1, respectively inducing induced pluripotent stem cells in an ectodermal direction and a mesodermal direction to obtain a neural embryoid and a yolk sac embryoid; s2, culturing the neural embryoid to perform neural differentiation to obtain a neural garland structure, and re-digesting the neural garland structure to obtain a neural progenitor cell single cell; culturing the yolk sac embryoid to enable the yolk sac embryoid to generate macrophage progenitor cells; and S3, fusing the neural progenitor cell single cell and the macrophage progenitor cell, and continuously culturing to form the 3D neural immune organoid containing the microglial cells. The brain-like organ provided by the invention has the functions of exploring the control of microglial cells on the progenitor cell proportion, the change of phagocytic function, the control on the number of mature neurons and the influence on cell proliferation and apoptosis in the development stage, and meanwhile, the brain-like organ can accept external stimulation and make stress change of corresponding functions.