26 results about "Neural differentiation" patented technology

The invention discloses a preparation method of composite microspheres for regulating and controlling a local inflammation microenvironment and promoting nerve regeneration. The method comprises the following steps: preparing liposome loaded with ginsenoside Rg1; preparing a GM microsphere loaded with a stromal cell-derived factor 1 alpha (SDF1 alpha); and preparing the inflammation regulation nerve regeneration promoting composite microspheres. The composite microsphere can reduce inflammatory response, reduce scar tissue formation, promote angiogenesis, continuously release SDF1 alpha and promote endogenous stem cell recruitment and neural differentiation. Therefore, the composite microsphere is a functional biological scaffold for firstly regulating and controlling local microenvironment inflammation of spinal cord injury and then promoting collection and differentiation of endogenous stem cells for innovative sequential inflammation regulation and nerve regeneration, and a new strategy is provided for treating spinal cord injury by tissue engineering.

The invention relates to the technical field of nerve cell differentiation, and discloses a culture medium for promoting neural differentiation of SH-SY5Y cells. The culture medium disclosed by the invention is prepared from the following components: tazarotene (tazarotene) and a basic culture medium. The culture medium can promote SH-SY5Y cells to present neuron-like differentiation within 5 days, significantly improves the length of neurite and the expression level of neuron markers, and is low in cytotoxicity. The invention provides an efficient, safe and standardized new tool for neural disease in-vitro model construction, drug screening and mechanism research.

The present invention relates to the field of cell biology, and more particularly to that of cell culture. The invention thus relates to a method for differentiating pluripotent cells into neural cells, adapted and optimized for three-dimensional cell culture. The neural cells obtained can, in particular, be used in cell therapy to treat neurodegenerative diseases, such as Parkinson's disease.

The invention relates to the medical field of urinary surgery, in particular to a fault diagnosis software system of an intelligent urinary surgery catheterization device, which is characterized in that an original data stream is collected by a unified timestamp, and normalized data is obtained through cross-brand semantic adaptation; utilizing inertial measurement exogenous input to suppress body motion artifacts and generating channel credibility; forming structured features based on second-order scattering and cross-channel attention; outputting pressure and flow velocity prediction under a physically constrained reversible and neural differential hybrid model, and inverting potential physical parameters; uncertainty calibration is carried out in combination with a brand-level calibration set, and a fault occurrence probability and a predicted failure time interval are given; a channel fault can be diagnosed and identified in cooperation with self-inspection and fault separation, and sound-light alarm and cross-terminal routing are achieved through arrangement of a system in a hospital. The system is suitable for bedside continuous monitoring and multi-brand deployment, and realizes robust diagnosis and short-term prediction under unified semantics.

The invention provides a whole-brain organoid based on human induced pluripotent stem cells as well as a construction method and application thereof, and belongs to the technical field of organoid construction. The method comprises the following steps: culturing human induced pluripotent stem cells in a complete culture medium to obtain a cell aggregate; culturing in an embryoid body forming culture medium to obtain a spherical embryoid body; culturing in a nerve induction culture medium tissue to form a nerve epithelium structure; embedding the single embryoid body with the nerve epithelium structure in a Matrigel gel drop, culturing in a nerve expansion culture medium to form a nerve epithelium-like structure, and replacing a mature culture medium for culturing to obtain the whole-brain organ. According to the method, by dynamically and accurately regulating and controlling culture parameters, the control ability of a cell differentiation area is improved, and interference of non-target cell types is reduced, so that the efficiency and consistency of embryoid formation and neural differentiation are directly improved, and finally, the obtained mature organoid has a more complete epithelial structure and cell polarity.

The application belongs to the field of stem cell biology and relates to a culture method of a 3D nerve immune organoid containing microglia cells, which comprises the following steps: S1, inducing induced pluripotent stem cells in ectoderm direction and mesoderm direction respectively to obtain nerve-like embryoid bodies and yolk sac-like embryoid bodies; S2, culturing the nerve-like embryoid bodies to make them differentiate into nerves to obtain nerve ring structures, re-digesting the nerve ring structures to obtain nerve progenitor cell single cells, and culturing the yolk sac-like embryoid bodies to make them generate macrophage progenitor cells; and S3, fusing the nerve progenitor cell single cells and the macrophage progenitor cells and continuing to culture to form the 3D nerve immune organoid containing microglia cells. The brain-like organoid of the application can realize the following functions: exploring the control of microglia cells on the proportion of progenitor cells in the development stage, the change of phagocytosis, the control on the number of mature neurons, the influence on cell proliferation and apoptosis, and simultaneously accepting external stimulation and making corresponding functional stress changes.

The technical problem addressed by the present invention is to provide a method for producing an apparent cross-karyocyte-like organ. This technical problem is solved by a method for producing a cross-seeded organ, said method comprising: (1) a step for culturing pluripotent stem cells in an intermediate medium between an undifferentiated maintenance medium and a neural differentiation medium; (2) a step for culturing the cells obtained in step (1) in a neural differentiation medium; and (3) a step for forming a cell mass prior to the start of step (2): (A) 6000 or more raw material cells are inoculated to form one cell mass, and (B) when the start of step (2) is set to the 0th day, the culture medium contains a Sonic Hedgehog signaling pathway acting substance from the 3rd day, and when the start of step (2) is set to the 0th day, the culture medium contains the Sonic Hedgehog signaling pathway acting substance from the 3rd day.

The invention discloses a neural network permanent magnet synchronous motor control method based on neural differentiation and a backstepping strategy, and belongs to the field of motor control, and the method comprises the steps: obtaining the sensing information of a permanent magnet synchronous motor, predicting the sensing information through a neural differentiation framework, and obtaining the nonlinear composite interference of a motor system; wherein the neural differential framework is a model combining a differential equation system and a neural network; according to the sensing information, a controller is constructed through a backstepping strategy, the input of the controller is an auxiliary variable and nonlinear composite interference, and the output of the controller is motor q-axis current data; and calculating the sensing information and the nonlinear composite interference through a controller to obtain neural network permanent magnet synchronous motor control information. According to the invention, the intellectualization and adaptive capability of the motor system under complex working conditions are further improved.

The invention discloses a method for promoting neural differentiation of mesenchymal stem cells by regulating a target gene through exosome miRNA, and belongs to the technical field of biology. The invention discloses a method for promoting neural differentiation of mesenchymal stem cells by regulating a target gene through exosome miRNA. The method comprises the following steps: S1, extracting the mesenchymal stem cells; s2, performing cryopreservation on the obtained mesenchymal stem cells; s3, taking out the lossless sampling cryopreservation tube; S4, culturing by using an MSC culture medium until the culture dish is fully paved with the MSC culture medium; s5, separating mononuclear cells; s6, obtaining neural stem cell-like cells; and S7, obtaining differentiated nerve cells. The invention solves the problem that the application of the mesenchymal stem cells in the treatment of immune-related diseases is influenced because the immune regulation ability of the mesenchymal stem cells cultured in the prior art is reduced, and can promote the proliferation and differentiation of the cells and improve the immune regulation ability of the mesenchymal stem cells.

The invention belongs to the field of regenerative medicine and cell therapy, and particularly relates to a flow shear force loading system and a method for promoting stem cell nerve differentiation by shear force. The flow shear force loading system comprises a liquid storage device, a peristaltic device, a flow stabilizing device and a parallel plate flow cavity device which are sequentially and circularly connected. According to the system and the method, the flow shear force can be accurately controlled by adjusting the flow speed of the fluid, and the system and the method have the advantages of low cost, no need of chemical additives and repeatability of operation. Expression of nerve-to-differentiation gene markers (such as Netin and NeuroD1) of stem cells can be remarkably improved within a short time (such as 4 hours) by utilizing flow shear force, so that the efficiency of nerve-to-differentiation is improved. Compared with a traditional chemical induction method which is 5-7 days long, the method is higher in efficiency, safer and more reliable. A novel and safer cell culture strategy is provided for clinical application of human dental pulp stem cells.

The invention provides inter alia a method for the production of an adherent cortical organoid, comprising the steps of: (a) providing a cell culture comprising a population of neural progenitor cells (NPCs); (b) seeding neural progenitor cells (NPCs) of said population in a well of a multiwell plate; wherein said well comprises a bottom surface (106) and an upright wall (104), and wherein a distance (x) between a centre (112) of the bottom surface and the upright wall is between 1.1 mm and 1.9 mm; and (c) culturing said seeded NPCs in a culture medium for neural differentiation for a period of time, to thereby allow formation of an adherent cortical organoid.

The present invention discloses a method for promoting neural differentiation of mesenchymal stem cells by regulating target genes through exosomal miRNA, and belongs to the field of biotechnology. The method for promoting neural differentiation of mesenchymal stem cells by regulating target genes through exosomal miRNA includes the following steps: S1: extracting mesenchymal stem cells; S2: freezing and storing the obtained mesenchymal stem cells; S3: taking out the non-destructive sampling cryopreservation tube; S4: culturing with MSC culture medium until the culture dish is covered; S5: isolating mononuclear cells; S6: obtaining neural stem cell-like cells; S7: obtaining differentiated neural cells. The present invention solves the problem that the mesenchymal stem cells cultured in the prior art will reduce the immunoregulatory ability of mesenchymal stem cells and affect their application in the treatment of immune-related diseases. The present invention can promote cell proliferation and differentiation and improve the immunoregulatory ability of mesenchymal stem cells.

The application belongs to the field of biomedical science and neurology, and particularly relates to application of CHCHD2 gene and a protein coded by the CHCHD2 gene in preparation of a product for promoting proliferation and differentiation of neural stem cells. The application proves that the CHCHD2 gene and the protein coded by the CHCHD2 gene are highly expressed in in-vivo neural development and in-vitro neural differentiation process; and that knocking down or over-expressing the CHCHD2 gene or the protein coded by the CHCHD2 gene has a certain regulating effect on proliferation and differentiation of neural stem cells. The application provides a new scheme for preparation of a product for promoting proliferation and differentiation of neural stem cells, and provides a new target for drug research and development of neural regeneration and repair treatment of central nervous system development deficiency diseases, neurodegenerative diseases, cerebrovascular diseases, craniocerebral and spinal cord injuries.

The invention discloses an Alzheimer's disease risk gene defect nerve cell as well as a preparation method and application thereof. The engineered induced pluripotent stem cell comprises an induced pluripotent stem cell which is deficient in ABCA7 gene and expresses a neural differentiation transcription factor in an inducible manner. According to the present invention, the Alzheimer's disease (AD) risk gene defect is introduced into the existing pluripotent stem cell line through the CRISPR and other gene editing means, and the inducible expression neural differentiation transcription factor is introduced through the lentiviral vector so as to establish the engineered AD risk gene defect pluripotent stem cell line, the engineered AD risky gene defect pluripotent stem cell line can be used for rapidly preparing nerve cells with Alzheimer's disease related A beta pathological phenotypes in a large scale by adding antibiotics for induced differentiation and screening culture, and is used for researching AD related pathogenesis and intervention means.

The present invention discloses the use of purple sweet potato pigment in the preparation of an induced stem cell neural differentiation product, which belongs to the field of biomedicine technology. The stem cell is a mesenchymal stem cell. The mesenchymal stem cell is a bone marrow mesenchymal stem cell. The neural differentiation is differentiation into acetylcholine neurons. The induced stem cell neural differentiation product includes a drug that induces stem cells to differentiate into neurons or a culture medium that induces stem cells to differentiate into neurons. The induction method is: pretreating stem cells with a growth factor mixture, and then adding purple sweet potato pigment to promote the differentiation of stem cells into neurons. The concentration of the purple sweet potato pigment is 50~100μg / mL. Through research, the present invention found that purple sweet potato anthocyanins can increase miR-223-3p, target Atg2b, inhibit the development of autophagy, and then induce BMSCs to differentiate into cholinergic neurons.

The invention provides colostrum derived stem cell populations, compositions and supplements having antimicrobial properties for enhancing mammalian health and for treating or preventing microbial contamination or infection. Also provided are methods of producing these populations, compositions and supplements from a female mammal in an inflammatory state. Also provided are colostrum derived stem cell populations and method of inducing differentiation into neural cells in neural progenitor medium including mammalian cell culture medium supplemented with casein depleted fraction of whey derived from colostrum. Also provided are neural progenitor medium, population of in vitro differentiated neural cells and pharmaceutical compositions containing same for use in treating neurological disease or disorder.

The present invention discloses a multi-channel cell-loaded nerve repair catheter with a double-patterned structure and a preparation method thereof. The preparation method comprises: ① preparing conductive fibers by wet spinning and in-situ surface polymerization to form conductive fiber bundles; ② designing a casting mold; ③ coating a release agent on the inside of the mold, and fixing the prepared fiber bundle in the mold, adding a UV-curable polymer solution to the mold, and curing it into a gel; ④ after the gel is aged, demoulding; after removing the surface release agent, using an immersion method to introduce a protein / polypeptide material that can promote cell adhesion on the surface of the nerve catheter, drying and sterilizing to obtain a multi-channel nerve repair catheter material with a double-patterned structure; combining the co-culture technology of neural stem cells, after the cells adhere and migrate in a directional manner in the tube, combining the pulsed direct current stimulation technology to achieve neural differentiation, and it can be used to replace autologous nerve transplant materials. The catheter material can be used in the fields of nerve and spinal cord repair.

The invention provides a therapeutic preparation for improving the nerve injury repair capacity of neural stem cells, and belongs to the technical field of neurobiology. The therapeutic preparation provided by the invention aims to improve the survival rate and neural differentiation capacity of neural stem cells during hypoxia treatment through two-stage pretreatment. The treatment preparation consists of a pretreatment culture medium 1 and a pretreatment culture medium 2, wherein the pretreatment culture medium 1 comprises a basic culture medium, N-acetylcysteine, Kunyuenin-1 polypeptide, an epidermal growth factor and a basic fibroblast growth factor; the pretreatment culture medium 2 comprises a basic culture medium, ligustrazine, astragalus polysaccharide and brain-derived neurotrophic factors. Experimental results show that the therapeutic preparation improves the survival rate of the neural stem cells in an anoxic environment and the expression level of a neural differentiation marker gene, and the effect of the therapeutic preparation is superior to that of single-stage treatment or direct mixing treatment, so that the neural stem cells can better realize damage repair of a nervous system.

The invention relates to the technical field of stem cell preparation and culture, and discloses a hospital-grade olfactory mucosa mesenchymal stem cell preparation and sterile culture process which comprises the following steps: collecting olfactory mucosa tissues from a donor olfactory mucosa area through a minimally invasive surgery, putting the collected tissues into a cold-chain transportation solution, and transporting at the temperature of 4 DEG C or below; a device for hospital-level olfactory mucosa mesenchymal stem cell preparation and sterile culture comprises a closed sterile bioreactor system used for controlling temperature, CO2 concentration, O2 concentration and pH value. Through electrochemical signal regulation and control, a real-time monitoring system and A I intelligent feedback, accurate control over cell proliferation, differentiation and anti-inflammatory functions is achieved, electric field parameters are optimized at different stages, cell proliferation, neural differentiation and anti-inflammatory capacity is improved, and through real-time monitoring and automatic feedback, it is ensured that the culture process is efficient, accurate and automatic, and the culture efficiency is improved. The cell culture success rate is obviously improved.

The present invention discloses a multi-layered cell-loaded nerve repair conduit with the functions of conducting electricity, resisting inflammation and promoting nerve differentiation, and a preparation method thereof. The preparation method is as follows: 1) preparing PLGA fibers doped with graphene, weaving them to obtain a layer of cylindrical fiber mesh; 2) preparing a HAMA mixed solution mixed with microspheres loaded with nerve growth factor and manganese dioxide nanoparticles, and a HAMA solution loaded with neural stem cells; 3) fixing the cylindrical fiber mesh in a cylindrical groove mold, pouring the HAMA mixed solution, and freeze-drying after UV curing to obtain a two-layer porous composite scaffold; 4) immersing it in the HAMA solution loaded with neural stem cells, rapidly curing to form a three-layer cell-loaded gel layer, and culturing in vitro for 4-8 weeks to obtain a multi-layered cell-loaded nerve repair conduit. The nerve repair conduit has the functions of conducting electricity, resisting inflammation and promoting nerve differentiation, and provides a new strategy for the repair of nerve and spinal cord injuries.

The invention belongs to the field of stem cell biology, and relates to a culture method of a 3D neural immune organoid containing microglial cells, which comprises the following steps: S1, respectively inducing induced pluripotent stem cells in an ectodermal direction and a mesodermal direction to obtain a neural embryoid and a yolk sac embryoid; s2, culturing the neural embryoid to perform neural differentiation to obtain a neural garland structure, and re-digesting the neural garland structure to obtain a neural progenitor cell single cell; culturing the yolk sac embryoid to enable the yolk sac embryoid to generate macrophage progenitor cells; and S3, fusing the neural progenitor cell single cell and the macrophage progenitor cell, and continuously culturing to form the 3D neural immune organoid containing the microglial cells. The brain-like organ provided by the invention has the functions of exploring the control of microglial cells on the progenitor cell proportion, the change of phagocytic function, the control on the number of mature neurons and the influence on cell proliferation and apoptosis in the development stage, and meanwhile, the brain-like organ can accept external stimulation and make stress change of corresponding functions.

The invention discloses a multi-layer integrated cell-loaded nerve repair conduit with conductive, anti-inflammatory and neural differentiation promoting functions and a preparation method thereof. The preparation method comprises the following steps: 1) preparing PLGA fibers doped with graphene, and weaving the PLGA fibers to obtain a layer of cylindrical fiber net; 2) preparing an HAMA mixed solution mixed with microspheres loaded with nerve growth promoting factors and manganese dioxide nanoparticles, and an HAMA solution loaded with neural stem cells; 3) fixing the cylindrical fiber net in a cylindrical groove mold, pouring an HAMA mixed solution, and freeze-drying after UV curing to obtain a two-layer porous composite scaffold; and (4) dipping in a neural stem cell-loaded HAMA solution, quickly curing to form three cell-loaded gel layers, and culturing in vitro for 4-8W to obtain the multi-layer integrated cell-loaded neural repair conduit. The nerve repair catheter has the functions of conducting electricity, resisting inflammation and promoting neural differentiation, and a brand new strategy is provided for nerve and spinal cord injury repair.

The invention discloses an anti-inflammatory-regenerative porous nerve conduit based on a hot-melt template technology and a preparation method and application thereof. The preparation method comprises the following steps: (1) preparing a plurality of gelatin cylinders and a cylindrical pouring mold with a fine needle array at the bottom; 2) preparing nano manganese dioxide and a SilMA solution loaded with manganese dioxide by a hydrothermal method; (3) respectively inserting the gelatin cylinders into the needle-shaped array of a mold, pouring a composite SilMA solution, carrying out UV curing and freezing demolding, and removing the internal gelatin cylinders in combination with a hot melting technology to obtain a porous catheter; and 4) carrying out in-situ auto-polymerization on the surface of the conduit to form a dopamine coating, and dipping and adsorbing a nerve growth promoting drug to obtain the required anti-inflammatory-regenerative porous nerve conduit material. The nerve conduit can promote neural stem cells, internal ingrowth and neural differentiation, meanwhile, neuroinflammation is prevented, and a brand new treatment strategy is provided for nerve injury.

The invention discloses application of purple sweet potato pigment in preparation of a product for inducing neural differentiation of stem cells, and belongs to the technical field of biological medicine. The stem cells are mesenchymal stem cells. The mesenchymal stem cells are bone marrow mesenchymal stem cells. The neural differentiation is differentiation to acetylcholinergic neurons. The product for inducing neural differentiation of the stem cells comprises a medicine for inducing the stem cells to be differentiated into neurons or a culture medium for inducing the stem cells to be differentiated into the neurons. The induction method comprises the following steps: pretreating the stem cells with a growth factor mixed solution, and then adding purple sweet potato pigment to promote the stem cells to differentiate into neurons. The concentration of the purple sweet potato pigment is 50-100 [mu] g / mL. Researches find that the purple sweet potato anthocyanin can target Atg2b and inhibit autophagy development by increasing miR-223-3p, and then BMSCs are induced to be differentiated into cholinergic neurons.

The present invention relates to a bioactive polypeptide synthesized on the basis of histone demethylase, and the use thereof in the neural differentiation process of mesenchymal stem cells. Disclosed in the present invention are possible protein-protein interaction binding sites of histone demethylase KDM2B and histone methylate EZH2, and disclosed are the roles of KDM2B and the bioactive polypeptide synthesized on the basis of the same in the neural differentiation process of mesenchymal stem cells and the tissue regeneration of injured spinal nerves. On this basis, it is concluded that KDM2B and the bioactive polypeptide synthesized on the basis of the same may play a role in promoting the neural differentiation of stem cells from apical papilla and the regeneration of injured spinal nerves.

The present invention belongs to the field of regenerative medicine and cell therapy, and specifically relates to a flow shear force loading system and a method for promoting neural differentiation of stem cells by shear force. The flow shear force loading system includes a liquid storage device, a peristaltic device, a flow stabilization device and a parallel plate flow chamber device which are connected in a circular manner. The system and method of the present invention can accurately control the magnitude of the flow shear force by adjusting the flow rate of the fluid, and its advantages include low cost, no need for chemical additives and repeatability of operation. The use of flow shear force can significantly increase the expression of neural differentiation gene markers (such as Nestin and NeuroD1) of stem cells in a short time (such as 4 hours), thereby improving the efficiency of neural differentiation. Compared with the traditional chemical induction method that takes up to 5 to 7 days, this method is not only more efficient, but also safer and more reliable. This provides a new and safer cell culture strategy for the clinical application of human dental pulp stem cells.