1439 results about "Polylysine" patented technology

Compositions comprising a glycosaminoglycan (e.g., a hyaluronan, hyaluronic acid, hyaluronate, sodium hyaluronate, dermatan sulfate, karatan sulfate, chondroitin 6-sulfate, heparin, etc.) in combination with at least one component selected from; i) polyglycols (e.g., polyethylene glycol), ii) long chain hydroxy polyanionic polysaccharides (e.g., dextran, sodium alginate, alginic acid, propylene glycol alginate, carboxymethyl cellulose and carboxyethyl cellulose, hydroxyl ethyl starch, hydroxyl propyl methyl cellulose, hydroxy propyl ethyl cellulose, hydroxy propyl cellulose, methyl cellulose, polylysine, polyhistidine, polyhydroxy proline, poly ornithine, polyvinyl pyrolidone, polyvinyl alcohol, chitosan, etc.) and iii) long chain Nitrogen containing polymers (e.g., Polylysine, Polyvinylpyrrolidone, and polyvinyl alcohol). The invention also includes methods for using such compositions (e.g., as substance delivery materials, tissue fillers or bulking agents, as moistening or hydrating agents, etc.)

The invention provides compositions for the treatment of disorders characterized by dryness including dry eye and dry mouth. The compositions commonly comprise a conjugate of hyaluronic acid and polylysine. These conjugates are attached to affected body tissues or surfaces using transglutaminase, and preferably endogenous transglutaminase.

This invention relates to substrates for use in immobilizing biomolecules. More particularly, the invention relates to substrates (e.g. glass slides) having a coating of polylysine covalently attached to a silane layer coating the slide, wherein the polylysine compound has a functional NH2 group which can be coupled directly, indirectly, covalently, or non-covalently to a biomolecule (e.g., a DNA or RNA molecule). Even more particularly, the invention relates to specific prescribed addition of ethanalomine to the polylysine thereby forming a mixture which dramatically enhances the effectiveness of the polylysine for immobilizing DNA. Among other applications, the polylysine coated substrates can be used in the preparation of high density arrays for performing hybridization assays

A mixed micellar aerosol pharmaceutical formulation includes a micellar proteinic pharmaceutical agent, an alkali metal lauryl sulphate, at least three micelle forming compounds, a phenol and a propellant. The micelle forming compounds are selected from the group consisting of lecithin, hyaluronic acid, pharmaceutically acceptable salts of hyaluronic acid, glycolic acid, lactic acid, chamomile extract, cucumber extract, oleic acid, linoleic acid, linolenic acid, monoolein, monooleates, monolaurates, borage oil, evening of primrose oil, menthol, trihydroxy oxo cholanyl glycine and pharmaceutically acceptable salts thereof, glycerin, polyglycerin, lysine, polylysine, triolein, polyoxyethylene ethers and analogues thereof, polidocanol alkyl ethers and analogues thereof. The amount of each micelle forming compound is present in a concentration of from 1 to 20 wt./wt. % of the total formulation, and the total concentration of micelle forming compounds are less than 50 wt./wt. % of the formulation. The propellant, e.g. a fluorocarbon propellant, provides enhanced absorption of the pharmaceutical agent.

A liquid formulation for contact lenses which comprises polylysine, polyphosphoric acid and/or salt thereof as a substance for suppressing the adhesion of polylysine to a contact lens, nitrogen-containing organic anti-microbial agent excluding polylysine and water. Contact lenses can be cleaned, disinfected and stored simply by soaking them in the liquid formulation without cleaning by digital rubbing or without rinsing before they are worn in eyes.

The invention discloses a biological compatibility surface coater and self-assembling coating method of planted medical apparatus, which is characterized by the following: the coater is prepared by self-assembling coating method layer by layer, which accelerates esoderma and resists thrombus constituted by two or more macromolecular materials with certain positive and negative load; the positive load material is chitose, chitin, polylysine and so on; the negative load material is disebrin, hyaluronic acid, alginic acid, polyamino acid and so on; the crosslinker and biological active factor or drug can be added to accelerate the growth of the cell; the coater satisfies the request of biologically medical transplantation.

The invention relates to a preparation method of a biological mimetic and structural bionic tissue adhesive in order to overcome disadvantages of present technological technologies for preparing mussel mucoprotein bionic biological tissue adhesives and improve the viscosity, the biocompatibility and the structural bionic property of a material. The preparation method comprises the following steps: grafting a natural polymer material with dopamine or other catechol group-containing derivatives through an amidation reaction by adopting DMTMM as a carboxyl group activator; and further modifying the grafted natural polymer material with lysine or polylysine by adopting the same technology to obtain the novel biological mimetic and structural bionic tissue adhesive containing dopamine analog and polylysine analog. The tissue adhesive plays a full role in tissue adhesion in wet and physiologic environment, has good tissue adherence and high biological safety, and can be slowly degraded in the tissue healing process until complete degradation. The preparation method of the biological adhesive has the advantages of simplicity, mild reaction conditions, increase of the reaction efficiency, improvement of the operability of the enterprise production process, and facilitation of amplified production of enterprises.

The invention discloses a method for immobilizing VEGF-carried heparin/polylysine nanoparticles on a Ti surface, which comprises the following steps: by utilizing the characteristic that Hep and PLL can produce static interactions to form nanoparticles, mixing VEGF-carried Hep and PLL to form VEGF-carried nanoparticles; preparing a DM coating on the Ti material surface; and by utilizing the characteristic that the DM and primary amino group can generate Michael addition and Schiff base reaction, immobilizing the amino-containing nanoparticles onto the sample surface in a covalent mode, thereby constructing the biologically modified surface with anticoagulation and endothelium promotion functions. By constructing a nanoparticle modifying layer with anticoagulation and endothelium promotion characteristics on the Ti surface, the invention obviously improves the blood compatibility and endothelium injury restorability of the material.

The present invention relates to novel compositions comprising microspheres and/or nanospheres containing condensed polyanionic bioactive agents, such as DNA. The polyanionic bioactive agent in the microspheres and/or nanospheres is preferably condensed using a polycationic condensing agent, such as poly-L-lysine. The present invention further relates to methods for producing the microspheres and/or nanospheres containing condensed polyanionic bioactive agents.

The invention discloses a chitosan-galangal essence microcapsule which comprises a core material and a wall material, wherein galangal essence is taken as the core material, and the wall material is formed through complex coacervation of chitosan, polylysine and sodium caseinate; and the core material is covered inside the wall material. The invention further discloses a preparation method of the chitosan-galangal essence microcapsule and application of the microcapsule in a mosquito repellent and a textile antibiotic finishing agent. The chitosan-galangal essence microcapsule disclosed by the invention can be used for improving the light and heat stability of the galangal essence, and has the advantages of high intensity, good heat stability, long and lasting mosquito repelling and antibiotic effects, and the like.

The invention provides a sustained-release composite microbial fertilizer and a preparation method thereof. The fertilizer comprises a fertilizer core and an envelope wrapping the fertilizer core, wherein the fertilizer core contains polyacrylamide, epsilon-polylysine, ascorbic acid, EDTA (ethylene diamine tetraacetic acid), a composite microbial agent and the balance being organic filler. The fertilizer is stable and efficient, and can be easily absorbed and utilized by crops, and damage and pollution to soil can be effectively avoided.

The invention relates to drug-loading nano-micelles, and a preparation method and an application thereof. According to the to drug-loading nano-micelles, a polyethylene glycol derivative-polylysine-polyleucine amphiphilic triblock copolymer is adopted as a carrier, and self-assembly is carried out in a water solution, such that nano-micelles with a three-layer structure transferring gene and medicine are formed. With the hydrophobic effect between polyleucine, the copolymer forms a core of the micelles in water, such that a hydrophobic medicine docetaxel is entrapped. With polylysine, the nano-micelles are positively charged, such that negatively charged anti-apoptotic protein small interfering RNA can be bonded. Polyethylene glycol is used for protecting the stability of the nano-micelles loading the medicines and gene, and a cycle time of the nano-micelles in vivo is prolonged. The nano-micelles are used for loading both an anti-apoptotic protein small interfering RNA gene medicine and a docetaxel medicine, such that the gene treatment medicine and the chemotherapeutic medicine cooperates in treating cancer, and a synergetic effect of the two medicines can be developed. Therefore, a direction is provided for better treatment of breast cancer.

The invention discloses a chitosan/polylysine in-situ gel and a preparation method thereof. The gel is an in-situ gel prepared from 1-5.5 percent by weight of sulfhydrylization chitosan and 0.1- 3.2 percent by weight of maleimide polylysine. The preparation method comprises the following steps of synthetizing the polylysine containing maleimide double bonds, and preparing a maleimide polylysine solution by taking a PBS (phosphate buffer solution) as a solvent; preparing a sulfhydrylization chitosan solution by taking the PBS as a solvent; and uniformly mixing the two solutions under the condition of physiological pH value to obtain the in-situ gel. The invention has the advantages that the preparation process is simple, and the prepared in-situ gel simulates the components, the structure and the functional characteristics of polysaccharide/polypeptide of a natural extracellular substrate, helps to increase histocompatibility of a material, promotes cell adhesion growth and has wide application prospects in the fields of tissue engineering and drug release.

The invention discloses a novel high-strength hydrogel which is prepared by the following steps: synthesizing a first network hydrogel from a polyelectrolyte (such as hyaluronate, polyglutamic acid, chondroitin sulfate or polylysine); and after soaking the hydrogel in a double-bond functional polyethyleneglycol water solution for some time, taking out, and crosslinking to obtain the high-strength double-network hydrogel. The synthesized high-strength hydrogel has favorable biocompatibility, can be used as a tissue engineering/repair scaffold material or the like, and has wide applicability in the field of biomedical materials.

The invention relates to the technical field of litchi preservation, and particularly discloses a room-temperature litchi storage preservative, and a preparation method and an application of the preservative. The preservative mainly comprises carboxymethyl-chitosan, nisin, natamycin, tea polyphenol, epsilon-polylysine, citric acid and phytic acid; litchi fruit is soaked in a preservation solution, and then aired, so that the litchi fruit can be stored at a room temperature; the quality and low level browning of the litchi fruit can be kept for a long time at the room temperature. Litchi treated by the preservative has the characteristics that the preservative is good in preservation effect, safe, environment-friendly, simple to operate and convenient to use; the preservative is extensive in source, low in cost, edible, and easy to degrade; no pesticide residue risk exists; short-term preservation during room-temperature storage and room-temperature logistics can be achieved; the preservative is a novel preservation material with a great development potential.

The present invention generally relates to nanobioelectronics and, in some cases, to circuits comprising nanoelectronic elements, such as nanotubes and/or nanowires, and biological components, such as neurons. In one aspect, cells, such as neurons, are positioned in electrical communication with one or more nanoscale wires. The nanoscale wires may be used to stimulate the cells, and/or determine an electrical condition of the cells. More than one nanoscale wire may be positioned in electrical communication with the cell, for example, in distinct regions of the cell. However, the nanoscale wires may be positioned such that they are relatively close together, for example, spaced apart by no more than about 200 nm. The nanoscale wires may be disposed on a substrate, for example, between electrodes, and the cells may be adhered to the substrate, for example, using cell adhesion factors such as polylysine. Also provided in other aspects of the invention are methods for making and using such devices, kits for using the same, and the like.

The invention relates to a nano micelle of a biodegradable macromolecular-bonding Pt(IV) anti-cancer medicament and a preparation method thereof. The structural formula of the anti-cancer medicament is defined in the specification, wherein the biodegradable macromolecule is tri-block copolymer, namely polyethylene glycol-b-polyester-b-polylysine; and tetravalency platinum coordination compound Pt(IV) is connected with a side amino on the block of polylysine on a macromolecular carrier through alpha, omega-polyethylene glycol. The carrier is non-toxic and is water-soluble, thus being convenient for reacting with the platinum(IV) coordination compound in water phase; a nano micelle form can be formed through self assembly; a platinum(IV) coordination compound is reduced to platinum(II), namely, cis-platinum, Carboplatin or Oxaliplatin, and the anti-cancer effect is known; the synthesis of the nano micelle is easy; the reduction potential of platinum(IV) is low, so that the platinum(IV) can be rapidly reduced to platinum(II) in cancer cells to take treatment effect; and the platinum(IV) is connected to the side chain of the macromolecule rather than the end of a chain, a macromolecular chain can be connected with multiple platinum(IV)s, and the content of platinum can be as high as 10-20%.

The invention discloses a preparation method and application of a carbon nanomaterial-immunostimulatory sequence compound. The preparation method comprises the steps of (1) modifying a carbon nanomaterial through polylysine to obtain a polylysine-modified carbon nanomaterial; and (2) mixing the polylysine-modified carbon nanomaterial obtained from the step (1) with an immunostimulatory sequence in a water solution, oscillating for 0.5-3 hours at 20-37 DEG C, and centrifuging, collecting and depositing to obtain the carbon nanomaterial-immunostimulatory sequence compound. According to the carbon nanomaterial-immunostimulatory sequence compound, the carbon nanomaterial serves as a carrier for intracellular transport of the immunostimulatory sequence (CpG DNA), so as to remarkably improve the cellular uptake efficiency of the CpG DNA, protect the CpG DNA from being degraded by nuclease and enhance the immunocompetence of an organism for a long time, so the carbon nanomaterial-immunostimulatory sequence compound has a good medical application prospect.

Disclosed is a device for sticking a plurality of cells to one basement in sequential arrays, which is characterized in that the upper surface of the basement is sequentially plated with a titanium adhesion layer, a golden layer, bands of mercaptan hydrophobic monolayer and bands of mercaptan hydrophilic monolayer, and the bands of mercaptan hydrophobic monolayer and the bands of mercaptan hydrophilic monolayer are parallel attached on the golden layer at intervals; the lower surface is provided with a second polydimethylsiloxane seal of a micro-groove unit; the micro-groove unit is composed of three parallel linear grooves; the ends of the grooves are provided with vertical through holes communicated with the grooves; the lower surface of the seal is coated on the surface of the basement with parallel bands of mercaptan hydrophobic monolayer and the bands of mercaptan hydrophilic monolayer; the linear grooves are perpendicular to the bands of mercaptan hydrophobic monolayer and the bands of mercaptan hydrophilic monolayer; and the golden surface of the basement is formed into a zone for the cells to be stuck on selectively. Substances (such as protein solution of the extra cellular matrix and polylysine solution, etc.), which facilitate the cells to be stuck, are injected into microflow pipes; after the cells are stuck selectively, different cell suspensions are injected into different pipes, and the cells are only stuck on the surfaces of the hydrophobic monolayer; then the seal is taken off, a plurality of different cell arrays are sequentially stuck on the basement.