720 results about "Polylysine" patented technology

The invention relates to a starch-based soft capsule shell and a preparation method thereof. The starch-based soft capsule shell is prepared from the following raw materials in parts by mass: 60 to 70 parts of hydroxypropyl starch, 9 to 20 parts of carrageenan, 5 to 15 parts of diquat, 5 to 15 parts of polylysine, 5 to 10 parts of hydroxypropyl methyl cellulose acetate succinate, 15 to 20 parts of glycerol and 30 to 40 parts of pure water. According to the soft capsule shell, hydroxypropyl starch serves as a base material, gelatin is not added, the conditions of poor weather resistance and fishy smell caused by use of gelatin are avoided, through the synergistic effect of all the components, the defects that a starch-based material is poor in plasticity, fragile and insufficient in mechanical strength are overcome, the structural stability is enhanced through chemical crosslinking, and the mechanical strength is improved; and the mechanical property is improved by virtue of physical compounding, so that the low-temperature resistance, high-temperature resistance and mechanical strength of the capsule skin are improved.

The invention discloses a compound feed for raising plateau live pigs and a preparation method thereof, and relates to the technical field of feed processing. The compound feed for raising the plateau live pigs is prepared from manganese-selenium compound modified charcoal, soybean polysaccharide polylysine-based supported nanoparticles, aspergillus oryzae, soybean meal powder, corn flour, wheat bran, mountain flour, calcium hydrophosphate, table salt and multivitamins. Manganese-selenium composite modified charcoal, soybean polysaccharide polylysine-based supported nanoparticles and aspergillus oryzae are subjected to co-fermentation culture to form a uniform, stable, loose and porous three-dimensional network fermentation substrate composite system, so that the damage of gastric acid to components is reduced, the targeted release of intestinal tracts is realized, and the bioavailability is improved. The composite functional additive which is high in nutrition, easy to absorb, anti-oxidation, anti-anemia, anti-ultraviolet radiation and the like is obtained, and the adverse effects of low temperature, oxygen deficit and ultraviolet factors on animal growth and feed oxidative rancidity are effectively reduced.

The invention discloses a preparation method and application of a composite PVDF-HFP and polylysine nanofiber diaphragm, and belongs to the technical field of lithium batteries. The composite material is prepared by blending polyvinylidene fluoride-hexafluoropropylene (PVDF-HFP) and polylysine and then carrying out an electrostatic spinning process. The PVDF-HFP is utilized to provide excellent mechanical strength and chemical stability, rich polar amino functional groups in a polylysine molecular chain are utilized to improve the lyophilic property of the membrane, and anions in an electrolyte are fixed through an intermolecular acting force, so that the transference number of lithium ions is increased, and the lithium ions are induced to be uniformly deposited. The nanofiber membrane combines the characteristics of PVDF-HFP and polylysine, has high porosity, good thermal stability and excellent interface compatibility, can effectively inhibit the growth of lithium dendrites and prevent the diaphragm from being punctured, and can significantly prolong the cycle life and improve the safety performance of the lithium metal battery when being applied to the lithium metal battery.

The invention relates to epsilon-polylysine epsilon-PL-loaded modified rice bran protein antibacterial nanoparticles as well as a preparation method and application thereof, and belongs to the technical field of preparation of nanoparticles. The preparation method comprises the following steps: extracting rice bran protein from defatted rice bran to prepare a rice bran protein stock solution; under continuous stirring, adding succinic anhydride into the rice bran protein stock solution to obtain acylated rice bran protein; mixing the acylated rice bran protein stock solution, the lactose stock solution and the epsilon-PL stock solution; and centrifuging and freeze-drying to obtain the epsilon-PL-loaded modified rice bran protein antibacterial nanoparticles. The preparation method disclosed by the invention is simple and low in cost, the prepared nanoparticles have smaller particle size and turbidity and higher stability, the hygroscopicity of epsilon-PL can be reduced to a great extent, the bioavailability of epsilon-PL can be improved, and the nanoparticles have an obvious bacteriostatic effect on escherichia coli and staphylococcus aureus.

The invention discloses an antibacterial anti-mite composite fabric and a preparation method and application thereof, and relates to the technical field of functional fabrics, the antibacterial anti-mite composite fabric comprises the following raw materials by mass: 10-20% of bamboo fiber, 10-20% of alginate fiber, and 60-80% of photo-thermal antibacterial modified cotton fiber; the photo-thermal antibacterial modified cotton fiber is prepared from the following raw materials in parts by weight: 1 to 2 parts of pretreated cotton fiber, 0.02 to 0.12 part of graphene oxide titanium dioxide composite material coated polydopamine-epsilon-polylysine antibacterial agent and 150 to 400 parts of water, the mass ratio of the pretreated cotton fiber to the graphene oxide titanium dioxide composite material coated polydopamine-epsilon-polylysine antibacterial agent is 1: (0.02-0.06). The composite fabric provided by the invention has excellent antibacterial rate and mite repelling rate, and also has excellent photo-thermal antibacterial and mite-killing characteristics.

The present invention relates to a composition (the composition of the invention) comprising a component A consisting of a polylysine component, an XL component and a fibrous component consisting of at least one fibrous element comprising plant fibers, and wherein the fibrous element is free of any other fibers other than plant fibers, and wherein the component A consists of a polylysine component, an XL component, and a fibrous component consisting of at least one fibrous element comprising plant fibers. And the composition does not contain any fibers other than the plant fibers of the fiber component. The invention further relates to a method for obtaining an object from the composition according to the invention. The invention further relates to objects (objects of the invention) obtained by said method, such as sheets, strips, bars, strips, films, cloths, containers, panels, panels, beams, frames, slats, engineered wood, such as fiberboards. The invention further relates to an article (article of the invention) comprising a) a component that is solid at 23 DEG C and 1 atm; and one or both of b) and c) wherein b) is the composition of the invention and c) is the object of the invention. The invention further relates to various uses of any one or any combination of the composition of the invention, the object of the invention and the article of the invention.

The invention provides a wound dressing for diminishing inflammation, easing pain and promoting wound healing and a preparation method thereof. The wound dressing capable of diminishing inflammation, easing pain and promoting wound healing is prepared from the following components in parts by mass: 6 to 10 parts of oxidized sodium alginate, 7 to 9 parts of hydrazide gelatin, 1 to 1.5 parts of tannic acid, 8 to 12 parts of polyethylene glycol, 0.05 to 0.1 part of cannabidiol, 0.05 to 0.1 part of neomycin sulfate, 0.2 to 0.3 part of 2-roots alcohol, 0.02 to 0.04 part of vitamin C palmitate and 0.03 to 0.1 part of epsilon-polylysine. The dressing can achieve excellent effects of diminishing inflammation, easing pain and promoting wound healing through synergistic cooperation of all the components.

The invention provides glycosaminoglycan hydrogel with a physical and chemical double crosslinking mechanism as well as a preparation method and application thereof, and relates to the technical field of biological materials. The glycosaminoglycan hydrogel is prepared from the following raw materials: oxidized sodium hyaluronate, chondroitin sulfate, epsilon-polylysine hydrochloride, a polydopamine-titanium dioxide compound and a stabilizer. The glycosaminoglycan hydrogel is prepared through a physical and chemical double-crosslinking mechanism under the specific raw materials and proportion, the problems of cytotoxicity and the like caused by use of a crosslinking agent or introduction of other solvents are avoided, a stable covalent network is constructed through chemical crosslinking, the gel is endowed with the self-repairing characteristic through physical crosslinking, and the self-repairing performance of the hydrogel is improved. And the viscosity of the product is remarkably improved, so that the hydrogel has stronger mechanical support performance, is suitable for wound repair, wound healing and the like, and has good antibacterial activity and stability.

The present invention relates to a composition (the composition of the invention) comprising a component A consisting of a polylysine component, an XL component and a fibrous component consisting of at least one fibrous element comprising plant fibers, and wherein the fibrous element is free of any other fibers other than plant fibers, and wherein the component A consists of a polylysine component, an XL component, and a fibrous component consisting of at least one fibrous element comprising plant fibers. And the composition does not contain any fibers other than the plant fibers of the fiber component. The invention further relates to a method for obtaining an object from the composition according to the invention. The invention further relates to objects (objects of the invention) obtained by said method, such as sheets, strips, bars, strips, films, cloths, containers, panels, panels, beams, frames, slats, engineered wood, such as fiberboards. The invention further relates to an article (article of the invention) comprising a) a component that is solid at 23 DEG C and 1 atm; and one or both of b) and c) wherein b) is the composition of the invention and c) is the object of the invention. The invention further relates to various uses of any one or any combination of the composition of the invention, the object of the invention and the article of the invention.

The invention relates to a heparinized gelatin / sodium alginate pancreas islet packaging material with an anti-fibrosis function as well as a preparation method and application of the heparinized gelatin / sodium alginate pancreas islet packaging material. The preparation method comprises the following steps: adding gelatin into normal saline, soaking, performing autoclaved sterilization to obtain a gelatin solution, then adding epsilon-polylysine hydrochloride into the gelatin solution, and finally adding glutamine transaminase for reaction to obtain an epsilon-polylysine grafted gelatin solution; adding heparin into the solution, and reacting to obtain a heparinized gelatin solution; adding sodium alginate and glutamine transaminase, reacting, diluting, preparing hydrogel microspheres by adopting an electrostatic spraying method, and cross-linking to obtain the hydrogel microspheres. According to the invention, heparin molecules are incorporated into a pancreas islet packaging system, so that fibrosis at the periphery of the microspheres can be relieved, the pancreas islet cells are packaged by using the pancreas islet packaging material to prepare the biological artificial pancreas, the pancreas islet cell proliferation and insulin secretion can be promoted, and the pancreas islet packaging material has a good diabetes treatment effect.

The invention relates to the technical field of polyolefin materials, in particular to a mildewproof and antibacterial polyolefin material and a processing technology and application thereof. The mildew-proof antibacterial polyolefin material comprises the following components in parts by weight: 60-80 parts of polypropylene, 5-15 parts of an elastomer, 2-5 parts of maleic anhydride grafted polypropylene, 15-25 parts of white oil, 10-30 parts of talcum powder, 1-5 parts of a mildew-proof antibacterial agent, 0.5-1.0 part of an antioxidant and 1-3 parts of a lubricant. The mildew-proof antibacterial agent is a mixture prepared from 0.5 to 2.0 parts of modified nano zinc oxide, 0.3 to 1.5 parts of methylisothiazolinone and 0.2 to 1.5 parts of epsilon-polylysine hydrochloride. The mildew-proof and antibacterial polyolefin material prepared by the invention has excellent mechanical properties and mildew-proof and antibacterial properties.

The invention relates to absorbable adhesive styptic powder and a preparation method thereof, and the absorbable adhesive styptic powder is characterized in that the absorbable adhesive styptic powder is formed by cross-linking aldehyde polysaccharide and a polyamino compound through Schiff base reaction, the raw materials are selected from at least one of glucan, sodium carboxymethyl cellulose, sodium hyaluronate, chitosan and carboxymethyl starch; the polyamino compound is selected from at least one of polylysine, polypeptide, bovine serum albumin and polyamine; the styptic powder is porous particles with the particle size of 10-1000 meshes, is cured into gel within 10 seconds after being in contact with body fluid, and is completely degraded within 15-60 days. According to the scheme, the styptic powder has the advantages of rapid wet adhesion, controllable degradation and excellent processability, and is an urgent clinical demand.

The present invention relates to the field of fiber materials, and discloses a UHMWPE composite fiber with a functional coating and a preparation method thereof. The composite fiber comprises a UHMWPE fiber bundle with a three-dimensional woven structure, and a phenolamine co-deposition modification layer, an inorganic functional nanoparticle deposition layer, and a silk fibroin / polylysine cross-linked porous layer sequentially coated on the surface of the fiber bundle. The present invention sequentially deposits phenolamine and NPs on the surface of the UHMWPE fiber bundle with a three-dimensional woven structure, then constructs a silk fibroin coating with a microporous structure on the surface of the inorganic functional nanoparticle deposition layer to coat the NPs to improve the stability of the NPs, and then self-assembles polylysine with an antibacterial effect with the silk fibroin through electrostatic adsorption, and finally forms a silk fibroin / polylysine cross-linked porous layer after cross-linking. The UHMWPE composite fiber of the present invention has excellent mechanical properties, stability, antibacterial properties and bone regeneration promoting properties.

The embodiment of the invention provides nano-enzyme hydrogel for promoting scar-free wound repair through inflammation regulation and c-Jun gene silencing and a preparation method of the nano-enzyme hydrogel. The nano-enzyme hydrogel is prepared from the following components in parts by mass: synthesized epsilon-polylysine modified CeO2 nano-enzyme particles and aldehyde hyaluronic acid, an instant Schiff base reaction between epsilon PL-CeO2 surface amino groups and HA-CHO molecule aldehyde groups is utilized, in-situ mixing is performed to form a hydrogel network, in addition, bFGF and siRNA are loaded into PLGA microspheres, and the hydrogel network is formed through in-situ mixing. And further embedding into the hydrogel to obtain the nano-enzyme hydrogel. The obtained nano-enzyme hydrogel has the functions of inflammation regulation, cell proliferation promotion, vascular regeneration promotion, c-Jun gene silencing and the like, and scar-free healing of skin wounds is remarkably promoted.

The invention belongs to the technical field of biomedical materials, and particularly relates to a biological adhesive with stable performance and a preparation method and application thereof. Specifically, by regulating and controlling the mixing mode of the active ester and the amino cross-linking agent, an acidic polylysine hydrochloride aqueous solution is adopted as the amino cross-linking agent, the active ester is directly dissolved in the polylysine hydrochloride aqueous solution, and the formed solution mixing system is stable in performance, can be stably stored for 6 hours at room temperature, can be prepared by an operator before an operation, and is free of secondary pollution. And the device can be immediately used at any time in the operation, has good batch stability, and avoids individual difference, so that the device has important application value and market prospect.

The present disclosure discloses a medical hydrogel, formed by in-situ crosslinking an aldehyde-terminated multi-arm star polyethylene glycol and a polyamino compound, wherein the aldehyde group and the multi-arm star polyethylene glycol are linked by a chemical bond such as an ether bond, an amide bond, an ester bond, a urethane bond, an imine bond, or a urea bond, and the molar ratio of the amino in the polyamino compound to the aldehyde group in the aldehyde-terminated multi-arm star polyethylene glycol is 0.4-4.4:1. The polyamino compound is polylysine or a mixture of polylysine and polyethylenimine in a molar ratio of 2-30:3. The hydrogel of the present disclosure gels rapidly, has a long-term stability in an aqueous solution, and still has good swelling property and stability after a plurality of radiations, and thus can be used as a medical radiotherapy protection spacer for radiation protection.

The invention discloses a special feed for breeding bumblebee pollination swarms and a preparation method thereof, and belongs to the technical field of insect feed preparation, and the special feed for breeding the bumblebee pollination swarms comprises the following raw materials: white granulated sugar, glucose, potassium sorbate, epsilon-polylysine, vitamins, trace elements and water; the preparation method comprises the following steps: adding the white granulated sugar and the glucose into boiling water, uniformly stirring, cooling, adding the vitamins, the trace elements, the potassium sorbate and the epsilon-polylysine, uniformly stirring, and cooling to room temperature. The components such as the sugar source, the vitamins and the preservative are introduced to achieve a synergistic effect, so that the freshness of the sugar water is guaranteed, the intake of nutrient substances by the bee colony is improved, the survival rate of the bee colony is effectively increased, the development time of the early-stage bear bee colony is shortened, the growth speed of the early-stage pollination bee colony is accelerated, and the colony potential of the bee colony before pollination is increased.

The invention relates to the technical field of biological materials, in particular to a PDRN porous microsphere as well as a preparation method and application thereof. The PDRN porous microspheres are prepared from the following raw materials: PDRN, an oil phase matrix, a curing agent and metal salt, the curing agent is at least one of polyethylene glycol acrylate and polylysine; the metal salt is at least one of calcium chloride, zinc chloride, magnesium chloride and ferric chloride. The PDRN porous microsphere has the advantages of being controllable in size, excellent in disintegration resistance and excellent in swelling resistance, is high in moist heat sterilization tolerance and strength, can be used as a filling material for medical beauty or an ideal candidate material of a tissue engineering scaffold, and has potential application value.

The invention discloses a catechol side group-containing polylysine derivative as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) dissolving catechol acid in an organic phase, firstly adding NHS, stirring and dissolving until the materials are uniformly mixed, then adding EDC.HCl, and stirring and reacting to obtain a catechol-NHS intermediate solution; (2) dissolving polylysine or a salt thereof in a water phase, and adjusting the pH value to 6.0-7.0 by using an alkaline solution to obtain a solution of polylysine or a salt thereof; and (3) adding the intermediate solution obtained in the step (1) into the polylysine or the salt solution thereof obtained in the step (2), and maintaining the pH value of the reaction system at 5.0-6.0 by using an alkaline solution to obtain the catechol side group-containing polylysine derivative. The preparation method is short in reaction time and mild in reaction condition, and the obtained product is high in grafting rate and high in raw material conversion rate and has a good application prospect in the aspects of tissue adhesives and the like.

The invention discloses a sodium iodide probe assisted gold nanoparticle-polyarginine-polylysine electrochemical sensor and an application thereof. The composite electrode provided by the invention comprises an electrode substrate and a nano composite film modified on the surface of the electrode substrate, the nano composite membrane comprises gold nanoparticles, polyarginine and polylysine. The invention further provides an electrochemical sensor which is constructed by combining the sodium iodide probe and the composite electrode and is used for detecting nitrite. The electrochemical sensor has the advantages of simplicity, rapidness, low detection limit, wide response range, good reproducibility, high selectivity and the like. The electrochemical sensor can be used for measuring the content of nitrite in beef and tap water samples, the recovery rate is satisfactory, and the electrochemical sensor has potential application prospects in the aspects of food safety and environmental protection.

The preparation method comprises the following steps: by taking natural chitosan, epsilon-polylysine and polyphenol as raw materials, firstly, respectively preparing polyphenol modified chitosan and carboxyphenylboronic acid modified polylysine through a carbodiimide coupling method, then, mixing single-component solutions of the polyphenol modified chitosan and the carboxyphenylboronic acid modified polylysine, and then, preparing the antibacterial and antioxidant glycopeptide hydrogel based on the polyphenol. And the antibacterial and antioxidant glycopeptide hydrogel is obtained by virtue of the synergistic effect of the phenolic hydroxyl group-boric acid group dynamic bond and the amino group-carboxyl group. The hydrogel disclosed by the invention has broad-spectrum antibacterial property and glucose response oxidation resistance, has self-healing capability and strong wound adhesion, is excellent in biocompatibility and can be naturally degraded; and the process is simple, convenient and controllable, large-scale production is easy to realize, the hydrogel can be used as a wound dressing for treating chronic difficult-to-heal wounds such as diabetes mellitus in a targeting manner, and integrated nursing of antibiosis, antioxidation, wound adhesion and self-repairing is realized.

The disclosure relates to compositions including a constituent—A, which constituent—A consists of a polylysine component, a XL-component, and a fibrous component which fibrous component consists of at least one fibrous element which fibrous element includes vegetable fibers, and where the fibrous element is free of any fibers other than the vegetable fibers, where and the composition is free of any fibers other than the vegetable fibers of the fibrous component. The disclosure further relates to processes for obtaining an object from the compositions. The disclosure further relates to objects such as sheets, tapes, sticks, strips, films, cloths, containers, boards, panels, beams, frames, planks, engineered wood e.g. fibreboards obtained by said processes.

The invention relates to the field of functional materials, and discloses a high-absorptivity sanitary napkin core material which is prepared from the following components in parts by mass: 30-35 parts of spiral nano cellulose; 20 to 25 parts by mass of a polyglutamic acid-polylysine grafted copolymer; 0.5 to 1.5 parts by mass of a thiodipropionic acid cross-linking agent; the helical angle of the helical nanocellulose is 25-35 degrees, the diameter of the helical nanocellulose is 5-10 nm, the specific surface area of the helical nanocellulose is greater than or equal to 300 m < 2 > / g, in the polyglutamic acid-polylysine grafted copolymer, the grafting rate of polylysine is 15-25%, the molecular weight of the polylysine is 3-5 kDa, the particle size of the nano-zinc oxide is 20-30 nm, the surface of the nano-zinc oxide is subjected to hydroxylation modification, and the surface of the nano-zinc oxide is 20-30 nm. The nano zinc oxide and the spiral nano cellulose are combined through a hydrogen bond and Van der Waals force, and the combination energy is greater than or equal to 25kJ / mol. By adopting the spiral angle and gradient pore design of the spiral nanocellulose, the technical effect of synergistic enhancement of liquid directional diversion and bottom layer latching is achieved.

The invention discloses a composition based on centella asiatica extract and olive extract and application of the composition in mask cloth, and relates to the technical field of cosmetics. Comprising the following raw materials: modified olive dispersion liquid, caprylic capric triglyceride, nano titanium dioxide dispersion liquid, chitosan quaternary ammonium salt, hyaluronic acid, modified centella asiatica extract, centella asiatica nanocrystals, silk fibroin composite liquid, epsilon-polylysine and deionized water. The prepared composition based on the centella asiatica extract and the olive extract effectively improves the transdermal absorption rate and stability of active ingredients such as asiaticoside and oleuropein in the extract, and can greatly improve the tensile strength, moisturizing time and skin adhesion of mask cloth when being applied to the mask cloth. Therefore, the composition based on the centella asiatica extract and the olive extract prepared by the invention has a wider application prospect.

The invention relates to the technical field of normal-temperature preservation of cooked meat products, in particular to a normal-temperature preservation method of cooked meat products based on natural components. The invention provides a method for improving the fresh-keeping degree of cooked meat products during normal-temperature storage, which comprises the following steps: applying a preset amount of compound preservative to the cooked meat products according to the expected fresh-keeping degree requirement, the compound preservative is prepared from epsilon-polylysine salt, nisin and sodium lactate. According to the method provided by the invention, the fresh-keeping degree of the cooked meat product can be comprehensively improved, so that the existing storage limitation is broken through while multiple requirements of consumers on safety, nutrition and flavor are met.

The invention belongs to the technical field of biomedical materials, and discloses a preparation method of nano composite hydrogel and application of the nano composite hydrogel in acceleration of healing of diabetic wounds. The invention constructs the hydrogel based on the epsilon-polylysine grafted by the oxidized hyaluronic acid and the phenylboronic acid and the ZIF-8. Due to the existence of the dynamic Schiff base, the boric acid ester bond and the coordination bond, the hydrogel has ideal mechanical property, strong viscosity, injectability, self-healing property and pH / ROS responsiveness. Due to the existence of chlorogenic acid and zinc ions, the hydrogel regulates macrophage phenotypes, relieves oxidative stress and accelerates healing of diabetic wounds through an NF-kappa B / JAK-STAT signal channel.

The invention discloses a methotrexate-cationic polypeptide conjugate as well as a preparation method and application thereof, and belongs to the technical field of medicinal chemistry. According to the conjugate, MTX and cationic polypeptide with the amino acid sequence shown as SEQ ID NO: 1 are connected through chemical bonding, the membrane binding characteristic of oligomeric lysine and the transmembrane capacity of TAT cell-penetrating peptide are creatively fused, and a polypeptide carrier system with efficient cell penetrating capacity is constructed; mTX obtains amphipathy and electropositivity through peptide fragment modification, stable nanoparticles can be spontaneously formed, and the problems of poor water solubility and instable acidity of raw material medicines are effectively improved; according to the conjugate, the accumulation efficiency of the medicine at a diseased region is remarkably improved by utilizing the targeting characteristic of the polypeptide carrier, the action time of the medicine is prolonged through a precise delivery mechanism, and meanwhile, the system toxicity is reduced. The preparation method adopts mild coupling reaction conditions, and the product is high in purity and excellent in stability; the compound is suitable for treating immune-mediated inflammatory diseases and specific malignant tumors, and provides an innovative solution thought for treating related diseases.

The invention discloses an algin composite membrane which is prepared by the following steps: adding 0.8-1.2 g of algin, 0.8-1.2 g of hydroxypropyl methyl cellulose, 0.8-1.2 mL of glycerol, 0.4-0.6 g of titanium dioxide and 0.8-1.2 g of polylysine into water, uniformly mixing, fixing the volume, putting into a container, drying, immersing into a CaCl2 solution for crosslinking treatment, and drying, thereby obtaining the algin composite membrane. The algin composite film can be used as or used for preparing a food preservative film. The algin composite film disclosed by the invention realizes good balance between strength and ductility, has good antioxidant capacity and remarkable antibacterial effect, and can effectively enhance the antioxidant defense capacity of the cape gooseberries, reduce oxidative damage, prolong the storage period and improve the fresh-keeping effect of the cape gooseberries. According to the method, the economic value of the sargassum is favorably improved, and powerful support is provided for sustainable development of the food industry.

The invention discloses a gamma delta T cell efficient amplification method and application, and belongs to the technical field of cell biology. The amplification method comprises the following steps: separating PBMC (peripheral blood mononuclear cells) by adopting a density gradient centrifugation method, carrying out initial culture through a polylysine coated container, carrying out three-stage dynamic stimulation, combining with an X-VIVO 15 culture medium of 5-8% autoserum, and finally carrying out anti-gamma delta TCR magnetic bead separation to obtain high-purity cells. Through collaborative optimization of stepped factor combination, staged container coating and a low-serum system, the amplification multiple of the gamma delta T cells reaches 150-180 times, the purity is larger than or equal to 90% after purification, the cytotoxicity and the survival ability are remarkably improved, the gamma delta T cells can be efficiently used for immunotherapy of tumors and infectious diseases, and stable technical support is provided for clinical transformation of the gamma delta T cells.