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Method for immobilizing VEGF-carried heparin/polylysine nanoparticles on Ti surface

A technology of polylysine and nanoparticles, which is applied in the field of nanoparticle preparation technology and surface modification of inorganic materials, can solve the problems of non-inhibition, Hep/PLL nanoparticle immobilization, poor hemocompatibility of titanium materials, etc., and achieve extended The half-life, preparation process and fixation method are simple and easy to operate, and the effect of reducing drug loss rate

Inactive Publication Date: 2012-09-19
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This is mainly due to the poor hemocompatibility of titanium materials, and has no inhibitory effect on vascular intimal hyperplasia and inflammatory reactions.
However, there is no relevant report on the immobilization of VEGF-loaded Hep / PLL nanoparticles on the surface of titanium materials.

Method used

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  • Method for immobilizing VEGF-carried heparin/polylysine nanoparticles on Ti surface
  • Method for immobilizing VEGF-carried heparin/polylysine nanoparticles on Ti surface
  • Method for immobilizing VEGF-carried heparin/polylysine nanoparticles on Ti surface

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Embodiment 1

[0023] see figure 1 , the first embodiment of the present invention is a method for immobilizing VEGF-loaded heparin / polylysine nanoparticles on the Ti surface, the steps of which are:

[0024] A. Sample preparation. Deposit a polydopamine coating on the polished pure Ti surface for use;

[0025] B. Preparation of Hep / PLL nanoparticles loaded with VEGF. An equal volume of VEGF solution (PBS, pH 7.4) with a concentration of 500 ng / ml was added dropwise to a heparin solution (PBS, pH 7.4) with a concentration of 20 mg / ml, and allowed to stand at 37° C. for 1 h. Then, under room temperature and magnetic stirring conditions, an equal volume of Hep and VEGF mixture was added dropwise to a PLL (MW 150,000-300,000) solution (PBS, pH 7.4) with a concentration of 1 mg / ml;

[0026] C. Nanoparticle immobilization. Soak the Ti sheet deposited with DM in step A in the nanoparticle suspension obtained in step B, react under shaking conditions at 15-50°C for 24 hours, rinse with PBS and ...

Embodiment 2

[0028] A method for immobilizing VEGF-loaded heparin / polylysine nanoparticles on the Ti surface, the steps of which are:

[0029] A. Sample preparation. Deposit a polydopamine coating on the polished pure Ti surface for use;

[0030] B. Preparation of Hep / PLL nanoparticles loaded with VEGF. An equal volume of VEGF solution (PBS, pH 7.4) with a concentration of 50 ng / ml was added dropwise to a heparin solution (PBS, pH 7.4) with a concentration of 5 mg / ml, and allowed to stand at 37° C. for 2 hours. Then, at room temperature and under magnetic stirring conditions, an equal volume of Hep and VEGF mixture was added dropwise to a PLL (MW 150,000-300,000) solution (PBS, pH 7.4) with a concentration of 0.2 mg / ml;

[0031] C. Nanoparticle immobilization. The Ti sheet deposited with DM in step A was soaked in the nanoparticle suspension obtained in step B, reacted at 50°C under shaking conditions for 6 hours, rinsed with PBS and double distilled water, and dried to obtain the targe...

Embodiment 3

[0033] A method for immobilizing VEGF-loaded heparin / polylysine nanoparticles on the Ti surface, the steps of which are:

[0034] A. Sample preparation. Deposit a polydopamine coating on the polished pure Ti surface for use;

[0035] B. Preparation of Hep / PLL nanoparticles loaded with VEGF. An equal volume of VEGF solution (PBS, pH 7.4) with a concentration of 200 ng / ml was added dropwise to a heparin solution (PBS, pH 7.4) with a concentration of 10 mg / ml, and stood at 37° C. for 3 h. Then, at room temperature and under magnetic stirring conditions, an equal volume of Hep and VEGF mixture was added dropwise to a PLL (MW 150,000-300,000) solution (PBS, pH 7.4) with a concentration of 0.5 mg / ml;

[0036] C. Nanoparticle immobilization. The Ti sheet deposited with DM in step A was soaked in the nanoparticle suspension obtained in step B, reacted at 37 °C under shaking conditions for 12 h, rinsed with PBS and double distilled water, and dried to obtain the target product.

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Abstract

The invention discloses a method for immobilizing VEGF-carried heparin / polylysine nanoparticles on a Ti surface, which comprises the following steps: by utilizing the characteristic that Hep and PLL can produce static interactions to form nanoparticles, mixing VEGF-carried Hep and PLL to form VEGF-carried nanoparticles; preparing a DM coating on the Ti material surface; and by utilizing the characteristic that the DM and primary amino group can generate Michael addition and Schiff base reaction, immobilizing the amino-containing nanoparticles onto the sample surface in a covalent mode, thereby constructing the biologically modified surface with anticoagulation and endothelium promotion functions. By constructing a nanoparticle modifying layer with anticoagulation and endothelium promotion characteristics on the Ti surface, the invention obviously improves the blood compatibility and endothelium injury restorability of the material.

Description

Technical field [0001] The invention relates to nanoparticle preparation technology and inorganic material surface modification technology, in particular to a biochemical modification method of artificial organ material titanium surface. Background technique [0002] Titanium (Ti)-based metal materials have been widely used in the biomedical field due to their good biocompatibility, but for some special applications, such as cardiovascular implant materials for the treatment of cardiovascular diseases, their biocompatibility Sex is far from meeting the clinical requirements. This is mainly due to the poor hemocompatibility of titanium materials, and has no inhibitory effect on vascular intimal hyperplasia and inflammatory reactions. [0003] By biochemically modifying the material surface, endowing the material with good anticoagulant ability and reendothelialization ability is an effective method to improve its biocompatibility. Heparin (Hep) is a common clinical anticoag...

Claims

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Application Information

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IPC IPC(8): C23C22/00A61L31/10A61L31/16A61L33/10
Inventor 陈俊英刘涛黄楠刘诗卉刘阳陈圆张琨陈佳龙冷永祥赵元聪
Owner SOUTHWEST JIAOTONG UNIV
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