2215 results about "Sample preparation" patented technology

This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In certain embodiments, the biological material is DNA and the genetic profile involves determining alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, an STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.

The present invention relates to fluidic devices for preparing, processing, storing, preserving, and/or analyzing samples. In particular, the devices and related systems and methods allow for preparing and/or analyzing samples (e.g., biospecimen samples) by using one or more of capture regions and/or automated analysis.

Systems and methods are provided for collecting, preparing, and/or analyzing a biological sample. A sample collection site may be utilized with one or more sample processing device. The sample processing device may be configured to accept a sample from a subject. The sample processing device may perform one or more sample preparation step and/or chemical reaction involving the sample. Data related to the sample may be sent from the device to a laboratory. The laboratory may be a certified laboratory that may generate a report that is transmitted to a health care professional. The health care professional may rely on the report for diagnosing, treating, and/or preventing a disease in the subject.

A method of the present invention comprises fractionating a sample solution containing analyte DNA molecules into small droplets, wherein the number M of the droplets is greater than the total number N of the DNA molecules, subjecting an emulsion containing the droplets to, for example, PCR amplification, and detecting the presence or absence (amount) of an amplicon obtained in each droplet by fluorescent detection using an intercalator or the like.

The present invention relates to methods and systems for microfluidic DNA sample preparation. More specifically, embodiments of the present invention relate to methods and systems for the isolation of DNA from patient samples on a microfluidic device and use of the DNA for downstream processing, such as performing amplification reactions and thermal melt analysis on the microfluidic device.

The invention discloses a multifunctional true triaxial rock drilling test system and a test method for characterizing the characteristics of rocks. According to the method, a test piece is drilled for several times to obtain different rock cores, then the rock cores are subjected to single axial tests and triaxial tests so as to obtain a plurality of sets of mechanical property parameters; then a true triaxial rock drilling tester that can directly measure the parameters while drilling is used to obtain a plurality of sets of parameters while drilling; a mathematical relationship between the mechanical property parameters and the parameters while drilling can be established, and thus the mechanical property parameters can be obtained only through measuring the parameters while drilling of rocks according to the mathematical relationship. According to the method, a multifunctional true triaxial rock drilling tester is taken as the preset test apparatus; the tester can perform true triaxial loading on a test piece and is capable of simulating the drilling process when a test piece is pressed from three directions in underground engineering; the relationship between the parameters while drilling and mechanical characteristics of rocks under different pressures on a test piece can be established, and the test method comprises specific steps from sample preparation to data processing.

The invention provides a portable rock mass structural plane direct shear test apparatus for field and indoor use. The apparatus comprises a sample preparation shear box and a shear test device. Specifically, the sample preparation shear box is mainly composed of a shear box housing, mould stripping pieces fixed by knobs and the shear box housing, and clamping pieces positioned at the ends of fixation screw rods that are mounted on the mould stripping pieces; the shear test device mainly consists of a shear test device outer frame, a vertical bearing plate, a horizontal pressure bearing instrument, sensors and jacks, with the vertical bearing plate mounted at the upper part of the shear test device outer frame and between a sensor and a jack, and the horizontal pressure bearing instrumentdisposed on a rolling shaft unit in the shear test device. Used in field or indoor rock mass structural plane direct shear tests, the apparatus of the invention can save sample preparation time, thusbeing suitable for rapid structural plane direct shear tests. The apparatus provided in the invention can improve the accuracy and convenience of structural plane direct shear tests, and has the advantages of simple structure, easy manufacturing, convenient operation and low cost, etc.

Design manufacturing and application of a triaxial penetration tester of the invention breaks through the limitation of permeability coefficient testing in Standard for soil test method GB/T50123-1999 and Specification of soil test SL237-014-1999. According to the invention, the tester is designed based on a varying-head principle; with water head with a height of up to 47 m provided by an air compressor, a constant head can be used to process the test results. Coarse grained soil and fine grained soil can be calculated both by a constant head and by a varying head, and the results are of the same order of magnitude and are quite close to each other. The tester of the invention comprises an air compressor, a penetration tester frame, a triaxial penetration pressure chamber, a liquid level sensor, a test data collector and a receiver, a data processing software, and a computer. Data are read on-line, are stored automatically, and samples of 10-9 cm/s or lower can be tested. The tester of the invention can test permeability coefficients of undisturbed soil, disturbed soil, and additive mixtures of soil and cement. The sample preparation method is simple and practical; the test is rapid and convenient, and covers the application fields which require permeability coefficient testing in soil mechanics or materials science.

A device and method for analyzing a sample, in particular a sample which contains low-density materials, is provided. Ions of a predefined mass and/or a predefined elementary charge are selected from a plurality of ions. The selected ions are directed onto the sample for sample preparation. An electron beam is then directed onto the prepared sample and a spatial distribution of scattered electrons is measured.

Integrated devices that include a sample preparation component integrated with a detection component are disclosed. The sample preparation component may be a digital microfluidics module or a surface acoustic wave module which modules are used for combing a sample droplet with a reagent droplet and for performing additional sample preparation step leading to a droplet that contains beads/particles/labels that indicate presence or absence of an analyte of interest in the sample. The beads/particles/labels may be detected by moving the droplet to the detection component of the device, which detection component includes an array of wells. Additonal analyte detection devices configured to operate an analyte detection chip to prepare a test sample and to detect an analyte related signal from the prepared test sample in the analyte detection chip are disclosed. The analyte detection chip may include a digital microfluidics (DMF) region and an analyte detection region which may overlap or may be spatially separated. The analyte detection device may be configured for detection of analyte by an optical or electrochemical means operably connected with an analyte detection chip inserted into the device.

The present invention provides an integrated, fully automated, high-throughput system for two-dimensional electrophoresis comprised of gel-making machines, gel processing machines, gel compositions and geometries, gel handling systems, sample preparation systems, software and methods. The system is capable of continuous operation at high-throughput to allow construction of large quantitative data sets.

The invention relates to a metallographic corrosion method for clearly displaying the original austenite grain boundary of a NiCrMoV type rotor steel, and belongs to the field of metallographic sample preparation. The method comprises the steps of: coarse grinding, fine grinding, polishing and corroding; putting a polished sample into a corrosive liquid of 75 DEG C, wherein the formula of the corrosive liquid comprises 200ml of distilled water, 5g of picric acid, 2ml of 5% diluted hydrochloric acid and 4g of sodium dodecyl benzene sulfonate; etching for 2minutes, taking the sample out, cleaning the sample with flowing water, cleaning the sample with alcohol and drying; and observing the clear original austenite grain boundary through an optical microscope. The method has the advantages that a difficult displaying problem of the original austenite grains of the NiCrMoV type rotor steel is solved, the original austenite grain boundary can be very clearly displayed, and the method is simple and practicable.

The invention describes a method for determining aggregation in protein, peptide or peptoid formulation, without the use of probes or additives. The method uses FTIR spectroscopy combined with the two-dimensional correlation analysis (2DCOS) which allows for the determination of the presence of aggregates, the determination of the mechanism of aggregation, allowing for correction in the pipeline manufacturing process of the protein to once again generate viable protein. In addition, the thermal transition of the protein can also be determined and a 2DCOS plot generated to compare with the established viable protein, allowing for quality control, stability and viability of the desired protein product. The ease of sample preparation and data analysis allows for the automation of this method.

A flow chamber (1) made of plastics as an object carrier for light-microscopic examinations comprised at least one channel (4) in a base plate (2), said channel having a width of 0.01 to 20 mm and a height of 0.01 to 5 mm. One liquid reservoir (3, 3′) each is connected to the inlet and outlet opening (6, 6′) of the channel, whereby a communicating system is generated. The bottom and/or the cover of this chamber is made of a high-class plastic material and may be functionalized. The inlet and outlet portions of the channel may be formed by a rounding (7) of the channel edges or by a surface treatment in a manner that drop formation is prevented and the flow is therefore not obstructed. In a method of sample preparation for light-optical microscopic examinations, a sample flow is generated by a system of communicating pipes, in that a reservoir of a solution with the sample is connected via a third channel with at least one further reservoir and the filling level of the reservoirs differs at the beginning of the examination.

An integrated lab-on-a-chip diagnostic system for carrying out a sample preparation process on a fluid sample containing cells and/or particles, the system comprising: (a) an inlet for a fluid sample; (b) a lysis unit for lysis of cells and/or particles contained in the fluid sample; (c) a nucleic acid extraction unit for extraction of nucleic acids from the cells and/or particles contained in the fluid sample; (d) a reservoir containing a lysis fluid; (e) a reservoir containing an eluent for removing nucleic acids collected in the nucleic acid extraction unit; wherein the sample inlet is in fluid communication with the lysis unit, an optional valve being present to control the flow of fluid therebetween; wherein the lysis unit is in fluid communication with the nucleic acid extraction unit, an optional valve being present to control the flow of fluid therebetween; wherein the reservoir containing the lysis fluid is in fluid communication with the lysis unit, an optional valve being present to control the flow of fluid therebetween; and wherein the reservoir containing the eluent is in fluid communication with the nucleic acid extraction unit, an optional valve being present to control the flow of fluid therebetween.