4076 results about "Sample preparation" patented technology

Methods and systems for sample preparation techniques that prevent inhibition of reactions such as due to the presence of longer oligonucleotides are presented herein. The methods and systems provided herein allow amplification (e.g., whole genome amplification) and sequencing of chromatin accessible regions of single cells.

The invention provides a sample preparation and determination method of an ore sample, and belongs to the field of process mineralogy, the sample preparation method of the ore sample comprises two times of wet screening to divide the sample into a sample 11, a sample 31 and a sample 41, the sample 11 and liquid glue are uniformly mixed, the mixture is heated and vibrated to remove bubbles, the mixture is heated and cured, side cutting, secondary inlaying and re-curing are performed, and a sample 12 is obtained; uniformly mixing the sample 31 with a thermosetting inlaying material, putting the mixture into an inlaying machine, and heating, pressurizing and curing to obtain a sample 32; uniformly mixing the sample 41 with resin particles, adding 3-5 mL of liquid glue at a low temperature, uniformly stirring and mixing, vacuumizing to remove bubbles, curing, adding the liquid glue again until the total volume is 10-15 mL, and curing to obtain a sample 42; and grinding, polishing and carbon spraying treatment are carried out on different samples respectively. Different sample preparation methods are selected according to different particle size distributions of the sample 11, the sample 31 and the sample 41, so that the samples with different particle size distributions are prepared more uniformly and have higher representativeness.

The invention provides a mineral leaching property analysis method, which belongs to the field of process mineralogy, and comprises the following steps: crushing and enriching a sample to be detected, and selecting single mineral particles to obtain target mineral particles; the preparation method comprises the following steps: coating a sample preparation grinding tool with epoxy resin glue, adding target mineral particles, and carrying out curing and grinding treatment to obtain a primary sample; cutting the primary sample, placing the grinding surface downwards and the three wear-resistant alloys at the bottom of a sample preparation grinding tool, injecting epoxy resin glue, curing to form a composite sample, accurately grinding, polishing, performing carbon spraying treatment, performing first automatic mineralogical analysis, measuring the area of a target mineral, cleaning, and performing leaching treatment to obtain a treated sample; and after solidification, accurate grinding and polishing and carbon spraying treatment, secondary automatic mineralogical analysis is carried out, the area of the residual target mineral is measured, and the leaching index of the target mineral is calculated. Reliable technical support is provided for mineral processing technology optimization and resource evaluation, and the method is particularly suitable for development of efficient leaching technologies of precious metal, rare earth and the like.

The invention relates to a solidified soil proportion and strength prediction method and system based on SEM image analysis, and belongs to the technical field of solidified soil strength testing. Comprising the following steps: S1, measuring physical indexes of disturbed soil; s2, multi-working-condition sample preparation and maintenance; s3, fractal dimension calculation of the SEM image is carried out; s4, unconfined compressive strength testing; s5, establishing a microstructure inversion model and a macroscopic strength prediction model; and S6, proportion optimization and strength prediction: utilizing the microstructure inversion model and the macroscopic strength prediction model to carry out solidified soil proportion optimization or strength prediction. According to the method, the quantitative relation between the microstructure (fractal dimension) and the macroscopic strength is established through SEM image analysis, the defects in the prior art are overcome, and a brand new technical path is provided for resource utilization of disturbed soil.

A sample preparation instrument with an integrated device for estimating a concentration of white blood cells in a specimen is described. The sample preparation instrument receives a specimen for which a sample is to be prepared for analysis. The integrated device can implement an optical method, an electrical resistance method, or a flow cytometry method, for example, to estimate the white blood cell concentration in the specimen. For some types of analysis, a sample volume of the specimen is dependent on the white blood cell concentration. Therefore, the sample preparation instrument can automatically determine and adjust the sample volume of the specimen based on the estimated white blood cell concentration prior to adding additional reagents, such as labeling reagents and / or lytic reagents, to prepare the sample.

The utility model discloses a tensile shear and stripping test piece sample preparation tool, which belongs to the field of adhesive detection and comprises a bottom plate, a tensile shear sample preparation component and a stripping sample preparation component, and the tensile shear sample preparation component and the stripping sample preparation component are arranged on the bottom plate. The tensile shear sample preparation assembly comprises a first test piece groove and a second test piece groove which are formed in the bottom plate, and the first test piece groove and the second test piece groove are formed in the first direction; the stripping sample preparation assembly comprises two guide stand columns arranged on the bottom plate, a plurality of pressing blocks are arranged between the two guide stand columns in a sliding mode in the second direction, and test piece mounting grooves are formed in the pressing blocks in the third direction; the first test piece groove and the second test piece groove are located between the two guide stand columns, the pressing block is located above the second test piece groove, and the first direction, the second direction and the third direction are perpendicular to one another. The device can be used for simultaneously preparing a tensile shear test piece and a stripping test piece, and the adhesive layer thicknesses of different adhesives can meet the standard requirements by adjusting the pressure.

A diagnostic test device to perform a test on a biological or environmental sample is provided. In one aspect, the diagnostic test device includes a chamber configured to receive a fluid from a sample preparation reservoir at a first section of the chamber, and a plurality of spaced apart valleys along an inner surface of the at least one chamber. Each of the plurality of spaced apart valleys include a curved cross-section and is configured to promote flow of the fluid toward a second section of the at least one chamber.

The invention relates to a focused ion beam ultrathin section sample preparation pretreatment method for a wafer sample, and belongs to the technical field of wafer material microscopic experiments. The wafer sample preparation pretreatment method provided by the invention comprises the following steps: (1) depositing a metal oxide on the surface of a wafer sample by adopting an atomic layer deposition technology to obtain an ALD layer; (2) coating a curing glue solution on the surface of the ALD layer, and curing to obtain a curing glue layer; and (3) plating a conductive protection layer on the surface of the cured adhesive layer to finish sample preparation pretreatment. The sample preparation pretreatment method disclosed by the invention can well solve the problems of irradiation damage and deformation of a fine structure which is not resistant to electron beam irradiation, curtain effect in a cutting process, unobvious structural boundary in a shooting process and the like in focused ion beam ultrathin section sample preparation, and has good application prospects in the technical field of wafer material microscopic experiments. And the practical value is very high.

The invention provides a TEM sample and a preparation method and application thereof.According to the preparation method of the TEM sample, on the premise that the hole structure of a semiconductor device is not affected, part of residual polymer accumulated and blocked in a hole opening in the semiconductor device is removed through reactive ion etching, so that the hole opening is expanded and not blocked any more; a protective colloid can enter a pore structure for filling and precursor material plating of atomic layer deposition is facilitated, the definition of the side wall of the pore is enhanced, and the quality and the final observation effect of the TEM sample are effectively improved; the equipment and technology adopted by the preparation method of the TEM sample provided by the invention are low in investment, and the operation process is simpler and faster than that of a Fab etching process; meanwhile, batch laboratory-scale sample treatment can also be realized; in addition, compared with a wet chemical corrosion cleaning technology for removing residual polymers, the problems of chemical reagent residues, chemical reactant pollution and the like at the bottom of the deep hole structure are avoided.

A sensing device is used to determine the risk and / or probability of an COPD-attack and / or asthma attack of a patient. The sensing device includes a collection device for the non-invasive collection of a biological sample and a biosensor for determining the existence and / or the amount of a biomarker in the biological sample, preferably without the need for sample preparation. The device also includes a control and evaluation device that determines a risk and / or probability of an COPD and / or asthma-attack based on the existence and / or measured amount; and a measuring arrangement and a method for the maintenance of said sensing device or measurement arrangement, and an output unit that outputs the risk / probability.

A method for analyzing a sample includes introducing the sample into a vessel including a cation-exchange resin, where the cation-exchange resin has a high affinity to one or more interferent species in the sample, directing the sample through the cation-exchange resin to thereby capture one or more interference species in the cation-exchange resin to purify the sample, and releasing the purified sample from the vessel. Additionally, a method for analyzing a sample includes receiving an image depicting a diagnostic test region and a color reference chart, converting a test region image portion of the image to an analytical color space, determining first and second coordinate values characterizing the test region image portion in the analytical color space, and providing a quantitative measurement of an analyte of interest in the sample based on the first and / or second coordinate values and a predictive formula based on the color reference chart.

A method for analyzing a sample includes introducing the sample into a vessel including a cation-exchange resin, where the cation-exchange resin has a high affinity to one or more interferent species in the sample, directing the sample through the cation-exchange resin to thereby capture one or more interference species in the cation-exchange resin to purify the sample, and releasing the purified sample from the vessel. Additionally, a method for analyzing a sample includes receiving an image depicting a diagnostic test region and a color reference chart, converting a test region image portion of the image to an analytical color space, determining first and second coordinate values characterizing the test region image portion in the analytical color space, and providing a quantitative measurement of an analyte of interest in the sample based on the first and / or second coordinate values and a predictive formula based on the color reference chart.

The invention provides a sample preparation method for microbiological detection, a sample, a detection method, a kit and application. The method comprises the following steps: a treatment step: taking an in-vitro host cell sample, and treating the in-vitro host cell sample with a host cell lysis reagent and nuclease to degrade host nucleic acid, the treatment being carried out in a liquid with a salt concentration of 10-600mM; a nuclease inactivation step: providing conditions to inactivate the nuclease; and obtaining microbial nucleic acid: extracting total nucleic acid to obtain the sample for detecting the microorganisms. According to the method disclosed by the invention, the interference of the host cell genome on the sample detection sensitivity can be effectively reduced in metagenome sequencing, and effective sequencing data of microorganisms in a detection result is improved, so that the positive detection rate of the microorganisms and the sensitivity of detecting the microorganisms are improved.

The invention discloses a sample preparation and characterization method of a rotation angle crystal interface, which is suitable for a sample with dislocation at the interface of two crystals, and comprises the steps of sample preparation and measurement of the relative rotation angle theta of the two crystals. A certain angle is formed between the cutting direction and the interface for sample preparation, a slice is taken out, and the obtained slice has three thin areas including a top material A crystal, a top material A / bottom material B crystal mixing area and a bottom material B crystal; measurement of the rotation angle theta: the electron beam direction is parallel to the rotation axis of the top sample during testing, and the rotation angle theta is directly measured by analyzing crystallographic information of the three thin areas. According to the method, the rotation angle of the top crystal relative to the bottom crystal can be accurately measured through TEM analysis of the three thin regions, and the error-tolerant rate of TEM sample preparation and analysis experiments is improved. In addition, the sample preparation technology can be used for monitoring the change of the physical properties of the sample in mechanics, thermotics, optics, electricity and the like along with the reduction of the size of the sample from the micron level to the nano level.

In an example, a method includes producing a set of projected sample characterization values associated with each of a plurality of processing axes and identifying a preferred processing axis based on the projected sample characterization values. In another representative example, a method includes extracting a sample specimen, producing a sample image of the sample specimen, identifying a preferred sample tilt angle, and rotating the sample specimen to an orientation corresponding to a preferred sample tilt angle. The method further includes processing the sample specimen to produce a lamella. In another representative example, a system includes a focused ion beam (FIB) system, a sample holder, a stage, and a controller operable to cause the stage to rotate the sample specimen relative to the ion beam axis such that the ion beam approaches the sample specimen along a preferred processing axis.

The invention relates to the technical field of metal material sample preparation, in particular to a preparation method for extracting carbon replica of steel for a high-carbon wire rod. The method specifically comprises the following steps: S1, preparing a sample; s2, immersing the sample into a nitric acid alcohol solution; s3, placing the sample in a sample chamber of a high-vacuum coating instrument, evaporating a carbon film with the thickness of 10-20 nm on the surface of the sample, and taking out the sample after vacuum is relieved; s4, performing electrolytic film removal by adopting an electrolytic polishing corrosion instrument; s5, after the carbon film falls off, the nitric acid alcohol on the carbon film is cleaned until the absolute ethyl alcohol in the weighing bottle is colorless; s6, the carbon film is transferred into deionized water, after the carbon film is fully unfolded, the carbon film is fished up through a transmission electron microscope and a copper net and placed on filter paper, and the carbon film is tightly attached to the copper net; and S7, baking the carbon film sample adhered to the copper mesh for 30-60 seconds, and placing the dried sample under an electron microscope for observation to complete the preparation of the steel extraction carbon replica sample for the high-carbon wire rod. The method has the advantages of high demolding speed, complete and firm carbon film form, simple configuration and low cost.

The invention provides an immunodetection method of a multi-index immune checkpoint detection kit, which belongs to the technical field of immunodetection and comprises three main stages of sample preparation, immune reaction and data analysis. The method comprises the following steps: firstly, carrying out centrifugal separation to obtain a plasma sample, and preparing a fluorescent microsphere coupled capture antibody and biotin labeled detection antibody compound; then carrying out immune reaction, forming a sandwich type immune complex through specific binding, and adding streptavidin marked by phycoerythrin for signal amplification. Detecting a fluorescence signal by adopting a flow cytometer to obtain an original data matrix; a contribution feature vector, a utility feature vector and an expression feature matrix are extracted through a feature decomposition algorithm, and a deep learning model of a total-score-total structure is established for data analysis. Finally, a detection report containing a quantitative result, an importance score and reliability evaluation is generated, and the technical problem that in the prior art, the analysis accuracy of a multi-index immune checkpoint detection result is insufficient is solved.

The invention discloses an analysis method for detecting short-chain fatty acid in table salt by headspace arrow-shaped solid phase microextraction-gas chromatography mass spectrometry, and belongs to the field of analysis and detection. According to the method, firstly, based on a headspace arrow-shaped solid-phase microextraction technology, polar short-chain fatty acids are extracted and enriched by using an extraction head of 100 [mu] m Polyacrylate, and then gas chromatography-mass spectrometry is used for detection, so that qualitative and quantitative detection of nine short-chain fatty acids such as acetic acid, propionic acid, isobutyric acid, butyric acid, 2-methylbutyric acid, valeric acid, 2-methylvaleric acid, 4-methylvaleric acid and hexanoic acid in the table salt can be realized. According to the method, the headspace arrow-shaped solid-phase microextraction technology is used for extracting the short-chain fatty acid in the table salt for the first time, the effects of interference removal, concentration and enrichment are achieved, and the detection limit of the method is reduced by about 2 orders of magnitude compared with that of a headspace-gas chromatography-mass spectrometry method. The method is simple in sample preparation, high in sensitivity, good in recovery rate and stability and suitable for accurate detection of the short-chain fatty acid in the table salt.

The invention relates to the technical field of food quality detection, in particular to a tea oil authenticity identification method based on an in-situ mass spectrum-machine learning coupling technology. Comprising the following steps: S1, preparing a sample; s2, acquiring mass spectrum data; s3, feature extraction: performing dynamic binning processing on the mass spectrum data, extracting feature peak information, and performing time dimension feature aggregation to form an initial feature matrix; s4, data preprocessing; s5, training a machine learning model; and S6, intelligent identification and visual output: inputting the mass spectrum original data of the tea oil sample to be detected into the trained multi-model integrated system, outputting an identification result through weighted voting or an optimal model strategy, and giving a confidence interval to quantify the reliability of the result.

The invention discloses a positive pressure antifouling gamma-ray spectrum automatic monitoring square cabin, and relates to the technical field of nuclear emergency monitoring and square cabins, the positive pressure antifouling gamma-ray spectrum automatic monitoring square cabin comprises a cabin body, a transition cabin is arranged in the cabin body, the transition cabin divides the interior of the cabin body into a sealed measurement cabin and a sample preparation cabin, and one side of the transition cabin is provided with an air-tight door capable of communicating the sealed measurement cabin with the sample preparation cabin; a sample conveying cabin used for allowing a sample to go in and out of the sealed measuring cabin and the sample preparation cabin is arranged on the side, away from the airtight door, of the inner wall of the transition cabin, the cabin body is overall designed into three areas, and the to-be-detected sample treatment area and the electric refrigeration high-purity germanium gamma-ray energy spectrometer are effectively isolated; filtered clean air is continuously introduced into the measurement cabin, the pressure in the sealed measurement cabin is maintained to be greater than the external pressure, the requirements of air cleanliness and positive pressure are ensured, and the background of the surrounding environment of the electric refrigeration high-purity germanium gamma-ray energy spectrometer is prevented from being changed after external radioactive pollution air enters the sealed measurement cabin, so that the measurement is prevented from being influenced.

The invention discloses core cutting and sample preparation equipment for engineering quality detection, which comprises a machine body and further comprises a driving assembly, the top of the machine body is fixedly connected with a workbench, the top of the back surface of the machine body is provided with a driving piece, the front surface of the driving piece is provided with a cutting disc, and the middle of the bottom in the workbench is provided with a driving seat, the driving assembly comprises a driving motor, the driving motor is fixedly connected to the middle of the front face of the workbench and provided with an output shaft, the output shaft of the driving motor is fixedly connected with a first lead screw, and the outer side of the first lead screw is in threaded connection with a first ball nut pair. According to the invention, the negative pressure adsorption plate is arranged to adsorb and fix a sample, and the negative pressure adsorption plate and the rubber clamping plate are both made of energy-absorbing materials, so that the fixed concrete sample can reduce vibration and impact of the cutting equipment in the cutting process, the service life of the equipment is prolonged, and meanwhile, the cutting efficiency is improved. And the stable cutting environment is also beneficial to reducing the abrasion and failure rate of cutting equipment.

The invention provides a rock mineral analysis test management method and system, and the method comprises the steps: collecting rock mineral sample multi-modal data, and constructing a digital twinborn model; constructing a geological knowledge map, and injecting and fusing the two knowledge to generate a virtual test data set; performing self-supervision pre-training by using the data set to obtain a self-adaptive mineral phase recognition model; deploying the model to a block chain-Internet of Things double-chain traceability system to obtain an identification result and an optimization instruction; and executing a sample preparation process and removing unqualified samples. According to the method, diffusion steps are adjusted through adaptive noise scheduling and a space-time alignment algorithm, data are integrated, a high-reality-sense virtual test data set is generated, and a self-supervised pre-training and knowledge distillation technology is combined, so that the model has space-time feature extraction and dynamic optimization capabilities; and finally, full-process closed-loop control is realized through a double-chain traceability system and a modular robot, and the recognition precision of a complex scene is improved.

The invention provides a spinning product elongation at break detection method. The spinning product elongation at break detection method comprises the following steps: a sample preparation and pretreatment step, a sample form measurement step and a mathematical modeling step; a step of calibrating tensile test equipment and setting parameters; a stretching test execution and data acquisition step; calculating and preliminarily analyzing the elongation at break; a stress and strain relation analysis step; a step of data stability analysis; an abnormal value detection and processing step; a step of result comprehensive analysis and report generation; according to the method, accurate analysis of the relationship between the yarn form and the stress and strain is realized, and the accuracy of a test result is improved; stability and abnormal value detection analysis is carried out on the data, so that the reliability and stability of the test data are ensured; and powerful support is provided for performance evaluation and improvement of the yarn. The method has feasibility and practicability, and the efficiency and accuracy of detecting the elongation at break of the spinning product can be remarkably improved.

The invention discloses a molten steel element content on-line detection system based on a laser-induced breakdown spectroscopy technology, and relates to the technical field of molten steel element content on-line detection, the molten steel element content on-line detection system comprises an excitation system, an acquisition system, a data preprocessing module and a component detection module; the excitation system is used for emitting laser to the molten steel surface to generate plasma; the acquisition system is used for collecting signal light radiated by the plasma; the data preprocessing module is used for carrying out baseline correction and noise reduction processing on the collected original spectral signals; and the component detection module comprises an element content prediction model based on a gating specific expert attention network LSEA-Net and is used for carrying out quantitative analysis on the preprocessed spectral data to obtain the element content of the molten steel. The technology has the advantages that sample preparation is not needed, online rapid analysis and multi-element synchronous detection are achieved, and the technology is suitable for the high-temperature environment and the like, and the requirement of a steelmaking site for real-time component detection is met very well.

The invention relates to the technical field of exploration and development of unconventional oil and gas reservoirs, in particular to an experimental device and method for quantitatively evaluating CO2-hydrocarbon-water interaction mixing and dissolving capacity of a water-containing gas reservoir. The experimental device comprises a vacuum pump, a constant-temperature air bath box, a sample preparation unit, an injection unit, a testing unit and a metering unit. The method comprises the following steps: injecting excessive pure CH4 into a sample preparation unit at a constant pressure, enabling a two-phase system of the pure CH4 and formation water to reach hydrocarbon-water phase balance, transferring the formation water of saturated hydrocarbon into a visual phase tester, injecting CO2 into the visual phase tester at a constant pressure, fully stirring fluid, standing for a period of time, carrying out ion chromatography analysis, and determining the content of CO2 in the formation water. According to the experimental device, the proportion of CO2 and the proportion of CH4 in undissolved gas are determined, an experiment is carried out through the experimental device, a CO2-hydrocarbon-water interaction miscibility theory system in the whole CO2 injection and burying process of the gas reservoir with water can be perfected, and the problem that the miscibility of CO2-CH4 in the CO2-hydrocarbon-water system under the high-temperature and high-pressure conditions is difficult to effectively and quantitatively evaluate is effectively solved.

The invention belongs to the technical field of quartz raw ore process mineralogy analysis, and particularly relates to a quartz raw ore process mineralogy analysis method which is a method for judging whether quartz raw ore can be used as high-purity quartz raw ore or not. The quartz raw ore is subjected to sample preparation, wherein a sample preparation method of a powder sample comprises the steps of crushing, wet grinding, filtering concentration and drying in sequence; carrying out chemical component analysis, phase component analysis and crystal structure analysis on the obtained powder sample; and carrying out electron probe microcell analysis, laser Raman spectrum analysis, lithofacies microscopic analysis and inclusion microscopic analysis on the obtained optical sheet. According to the quartz raw ore process mineralogy analysis method, the preparation method of the powder sample is simple, low in energy consumption and environmentally friendly, meanwhile, the obtained powder sample is slightly polluted by the outside, the obtained quartz raw ore process mineralogy analysis result can be closer to a true value, meanwhile, stable production of high-purity quartz sand can be ensured, and the production cost is reduced. And the loss of manpower, financial resources and material resources is reduced.

The invention relates to a partition loading drilling gas extraction dynamic simulation experiment device and method, and belongs to the field of coal mine safety. The device comprises an experiment cylinder body, a loading device and an air supply device. A hole sealing section confining pressure pressurizing device, an extraction section confining pressure pressurizing device and an axial pressurizing device are arranged in the experiment cylinder body, and the influence of coal body elasticoplastic deformation on permeability is truly reflected by accurately distinguishing stress loading of a hole sealing section and stress loading of an extraction section; the method comprises the following steps: analyzing a stress state, formulating an experimental scheme, preparing an experimental sample, simulating a loading experiment and analyzing experimental data. By combining drilling construction simulation and negative pressure extraction testing, gas emission rules and leakage characteristics under different hole sealing materials, processes and extraction parameters are systematically analyzed. The technology can accurately test the gas emission rule and the air leakage characteristic of drill holes with different hole sealing processes and hole sealing parameters under negative pressure extraction, and provides a scientific basis for optimizing the gas extraction process and improving the extraction efficiency.

The invention belongs to the field of EBSD sample preparation, and relates to a method for preparing a metal microwire longitudinal section EBSD sample, which comprises the following steps: thermally embedding a metal microwire to obtain a thermally embedded block; the bottom of the hot inlay block is polished, and after the position traces of the metal microwires are exposed, the inlay block is cut into a plurality of hot inlay small blocks; carrying out cold inlaying on the hot inlaying small blocks by adopting thermoplastic resin to obtain first cold inlaying blocks; carrying out grinding, mechanical polishing and vibration polishing on the to-be-tested surface of the first cold mosaic block to obtain a second cold mosaic block; and heating the second cold mosaic block, and taking out the metal microfilament hot mosaic block to obtain the metal microfilament hot mosaic block. The method disclosed by the invention is relatively high in efficiency, large in effective area, low in preparation cost and wide in applicability, and is particularly suitable for metal microwires which are made of various materials and have the diameter of 100-500 microns. Therefore, the problem of preparation of the longitudinal section EBSD sample of the metal microwire is efficiently solved with high quality.

The invention discloses an in-situ TEM (Transmission Electron Microscope) device sample preparation method based on a suspended thinning and parallel carrying technology, which can be used for preparing in-situ TEM samples of semiconductor two-end devices and three-end devices, and mainly solves the problems of unstable ohm contact, nonuniform thinning, low success rate and high time cost in the prior art. According to the invention, an FIB sample preparation technology is adopted, and stable and reliable ohmic contact between a sample electrode and an MEMS chip electrode is ensured through specific-angle sample extraction, suspended thinning, parallel carrying, circuit breaking treatment and an accurate electrode welding process. According to the sample prepared by the invention, the failure state of a device can be observed in real time under a transmission electron microscope, the sample has the advantages of high sample preparation success rate, good repeatability, stable electrical parameters and wide applicable chip variety, and the reliability and repeatability of in-situ TEM test data are remarkably improved.

The invention belongs to the technical field of isotope detection, and particularly relates to a plutonium isotope accelerator mass spectrometry measuring device based on positive ion ionization, which comprises a positive ion source for ionizing plutonium isotope to generate positive ions, the positive monovalent ions of the plutonium isotope accelerated by the accelerating tube enter the stripping system and then are stripped into multi-charge state positive ions, 3 + charge state plutonium isotope ions are selected by the analysis magnet for analysis, and after interference of various analysis fragments is eliminated by the electrostatic analyzer, the 3 + charge state plutonium isotope ions are measured by the detector. The ionization efficiency of the plutonium isotope is improved, the sample preparation process is simplified, and the in-situ analysis of the sample can be realized.