2045 results about "Butyric acid" patented technology

The present invention is directed to microparticles, to microparticle compositions containing the same, to methods of forming the same, and to uses for the same, including use for a vaccine, for raising an immune response, for treatment of a disease and for diagnosis of a disease. The microparticles comprise a biodegradable polymer, such as a poly(α-hydroxy acid), a polyhydroxy butyric acid, a polycaprolactone, a polyorthoester, a polyanhydride, or a polycyanoacrylate, and a detergent selected from a cationic detergent and an anionic detergent. The microparticles further comprise an antigen adsorbed on the surface of the microparticle.

The present invention relates to novel halohydrin dehalogenase polypeptides and the polynucleotides that encode them. These polypeptides are useful in the production of 4-substituted-3-butyric acid derivatives and vicinal cyano, hydroxyl substituted carboxylic acid esters. The invention also provides related vectors, host cells and methods.

The invention is a fibrin glue that avoids the use of fibrinogen and thus eliminates the need for premixing and premature clot formation. The fibrin glue of the invention comprises thrombin, thromboplastin and calcium and may have clotting Factors, VII, IX and X, and the like. The invention also comprises a biosealant for use with the fibrin glue without fibrinogen or for use alone. The biosealant is a two component mixture of gelatin/resorcinol and glyoxal/glutaraldehyde/4-(p-maleimidophenyl) butyric acid. The two components are mixed on use.

The invention relates to acid solution used for processing the magnesium alloy surface. The acid solution is water solution which contains acids, inhibitor and wetting agent, wherein, the acids are first acids or mixture of first acids and second acids; the first acids are selected from one type or a plurality of types among citric acids, oxalic acids, tartaric acids, methanoic acids, acetic acids, metacetonic acids, butyric acids, glutaric acids, phenylformic acids, benzene dicarboxylic acids, lactic acids, glycolic acids, glyoxylic acids and amino acids; and the second acids are hydrochloric acids and/or nitric acids. By adoption of the acid solution, the magnesium alloy surface can be fully activated; the membranous layer of a converting film which is formed on the magnesium alloy surface after chemical conversion process is compact, has erosion resistance and good binding force with a paint film. Moreover, the method is a environment-friendly method for processing the magnesium alloy surface.

The invention relates to a eubacterium and clostridium preparation and application of the same, in particular to a microecological preparation which is prepared for replenishing butyric acid bacteria and butyric acid produced by intestinal tract by taking single eubacterium, single clostridium or a eubacterium and clostridium composition as a main active composition, and application of the same in treating related diseases through butyric acid production, and belongs to the field of biological medicine.

The invention discloses a preparation method of one of components of a novel antihypertensive drug LCZ696, namely, sacubitril (sacubitril, AHU-377, I). The method comprises the following preparation steps: carrying out cyclization, addition, debenzylation, ring opening, esterification, amidation and the like on a chiral induciton reagent (S)-1-(alpha-aminobenzyl)-2-naphthol (S-betti Base) and 2R-methyl-4-oxo-butyric acid to prepare the sacubitril (I). The preparation method is available in raw materials, concise in process, economic and environmentally friendly, and is suitable for industrial production.

The invention discloses a method for preparing L-glufosinate-ammonium by use of amino acid dehydrogenase. The method comprises the following steps: taking 2-carbonyl-4-(hydroxymethylphosphonyl)butyric acid or a salt thereof as a substrate, taking a cell of the isolated glutamate dehydrogenase or in vitro expression glutamate dehydrogenase as a catalyst to perform reductive amination reaction under the condition of the existence of inorganic amino donor and reduced coenzyme, thereby acquiring the L-glufosinate-ammonium. The method disclosed by the invention is high in raw material conversion rate and high in yield, the product is easy to separate and purify, and the chirality purity is high; compared with the transaminase and like catalysis technology, the process is relatively simple, and the raw material conversion rate reaches up to 100%.

The invention discloses a method for producing L-2-aminobutyric acid, and the method comprises the following step: catalyzing L-threonine utilized as a raw material through an enzyme catalysis system consisting of threonine deaminase, L-amino acid dehydrogenase and coenzyme regeneration systems, thus producing the L-2-aminobutyric acid. The method for producing theL-2-aminobutyric acid has the advantages that the raw material is low in price, the property is stable, and the production cost of the L-2-aminobutyric acid can be greatly lowered, the conversion rate and product concentricity are high, no influence caused by byproducts exists, and the method is suitable for industrialization application.

A method for attracting bed bugs includes placing a climb-up pitfall trap apparatus that includes a chemical lure composition. The lure composition includes a combination of (a) L-lactic acid, and (b) at least one fatty acid or salt selected from the group consisting of (1) propionic acid, (2) butyric acid, and (3) valeric acid, and (c) 1-octen-3-ol, and (d) a suitable ketone, and (e) a suitable aliphatic sulfide. The trap may also include in conjunction with the lure composition a carbon dioxide source and/or a heat source to compose a lure arrangement.

The present invention is directed to a compound of 2-N halo-4-methylsulfonyl-butyric acid, such as 2-N chloro-4-methylsulfonyl-butyric acid, or a pharmaceutically acceptable salt or solvate thereof. The present invention is also directed to a pharmaceutical composition comprises the compound and a pharmaceutically acceptable carrier. The present invention is further directed to a method for treating inflammation or inflammatory-related disorders, bacterial infection, pain, or skin conditions, by administering 2-N halo-4-methylsulfonyl-butyric acid to a subject in need thereof.

The present invention relates to butyrate producing bacterial strains related to the species Buty{acute over (η)}coccus pullicaecorum to be used in the prevention and/or treatment of intestinal health problems. The present invention therefore provides methods and compositions that overcome the problems associated with the currently used methods for administering butyric acid in the treatment of intestinal health problems in humans and/or animals.

Methods are disclosed for forming heptan-4-one, and, optionally, heptan-4-ol, from fermentable sugars. The sugars are fermented using a bacteria or yeast that predominantly forms butyric acid. The butyric acid is subjected to catalytic ketonization conditions to form heptan-4-one, with concomitant loss of water and carbon dioxide. The heptan-4-one can be subjected to catalytic hydrogenation to form heptan-4-ol, an either of these can be included in gasoline compositions. In one aspect, the fermentable sugars are derived from lignocellulosic materials such as wood products, switchgrass, or agricultural wastes, which are delignified to form lignin, cellulose and hemicellulose. The cellulose and hemicellulose can be depolymerized to form glycose and xylose, either or both of which can be fermented by the bacteria. Thus, the methods described herein can convert biomass to a fuel composition or fuel additive, which can be used in a conventional gasoline engine, unlike traditional fuels such as ethanol or biodiesel.

A process for the conversion of carbohydrates from any of a number of sources into butanol and hexanol for fuel or chemical use is disclosed. The process includes conducting a homoacetogenic fermentation to produce an acetic acid intermediate which is chemically converted to ethanol. The ethanol and a remaining portion of the acetic acid intermediate are used as a substrate in an acidogenic fermentation to produce butyric and caproic acid intermediates which are then chemically converted to butanol and hexanol.

Fluorinated oligopeptides, especially those having 4,4-difluoro-2-amino butyric acid at the C terminus, may be effective inhibitors of hepatitis C virus NS3 protease. Examples of hexapeptides of the invention, optimized for binding in the S1 specificity pocket of the enzyme, may display IC50s at the sub-micromolar level. Embodiments of tripeptides of the invention, having a keto-acid group at the C-terminus are, likewise, potent inhibitors of NS3 protease.

The invention discloses a production method of L-glufosinate. The method includes the steps that with 2-carbonyl-4-(hydroxyl methyl phosphoryl) butyric acid and salt thereof being a substrate, isolated transaminase or a cell catalysis substrate of in-vitro expression transaminase reacts with an amino donor under the condition that the amino donor exists, so that L-glufosinate is obtained, wherein the amino donor is alanine, and the amino acid sequence of transaminase is shown in SEQ ID NO.1-3. With 2-carbonyl-4-(hydroxyl methyl phosphoryl) butyric acid and salt thereof being the substrate and alanine being the amino donor, the transamination reaction occurs through the specific transaminase catalysis substrate, the substrate can be completely converted into L-glufosinate, and the conversion rate of raw materials is high and can reach 100%.

Previous methods for culturing human embryonic stem cells have required either fibroblast feeder cells or a medium which has been exposed to fibroblast feeder cells in order to maintain the stem cells in an undifferentiated state. It has now been found that if high levels of fibroblast growth factor, gamma amino butyric acid, pipecholic acid, lithium and transforming growth factor beta are added to the medium in which the stem cells are cultured, the stem cells will remain undifferentiated indefinitely through multiple passages, even without feeder cells or conditioned medium.

The invention relates to method and equipment system for separating and recovering organic oxygen-containing compounds in a Fischer-Tropsch synthesis water phase. The equipment system is integrated by adopting twelve towers including a mixed acid cutting tower, an acetaldehyde rectifying tower, a methanol/ethanol dividing tower, a methanol extraction rectifying tower, a methanol rectifying tower, an acetaldehyde removing tower, an ethanol tower, a propanol concentration extracting tower, a propanol intermittent azeotropic distillation tower, a carboxylic acid extraction tower, a carboxylic acid intermittent rectifying tower and an extraction agent recovering tower matched with the carboxylic acid extraction tower for use. By applying the method and equipment system disclosed by the invention, more than ten kinds of organic oxygen-containing compounds such as acetaldehyde, acetone, methanol, ethanol, normal propyl alcohol, normal butanol, acetic acid, metacetonic acid, butyric acid, butanone and the like can be separated from raw materials; and the products can respectively reach the industrial purity. The method and equipment provided by the invention are economic, effective and reasonable; and according to the method and equipment, efficiency of Fischer-Tropsch synthesis industrial enterprises can be greatly increased, production cost is reduced and goal of clean production can be achieved.

The invention relates to a hydroxylated polyurethane water dispersoid for a waterborne soft-feel coating and a preparation method thereof. The hydroxylated polyurethane water dispersoid is the water dispersoid formed by the following steps of: taking the raw materials in parts by weight: 15 to 20 parts of polycarbonate diols, 15 to 25 parts of elastic 4-butanediol, 1 to 5 parts of pentaerythritol, 2 to 5 parts of hydroxylated dimethylolpropionic acid or dihydroxymethyl butyric acid, 10 to 20 parts of fatty group or alicyclic group isocyanates monomers and 0.1 to 1.0 part of catalysts and solvents; carrying out heating polymerization to obtain hydroxylated urethane resin; and then neutralizing a water dispersoid formed after salt forming, self emulsifying and the solvent removing, wherein the coating-4 viscosity change of the coating stored for 6 months is smaller than 5 percent. After the hydroxylated polyurethane water dispersoid prepared by the method is prepared into a double-ingredient waterborne soft-feel coating, better balance is obtained between the coating film soft feel and the coating film resistance on alcohol and contaminants, and the effect of the hydroxylated polyurethane water dispersoid is proved.

A construct that comprises a base material and a polyhydroxyalkanoate, wherein at least a part of the base material is coated with the polyhydroxyalkanoate, and the polyhydroxyalkanoate comprises a 3-hydroxyalkanoic acid unit other than 3-hydroxypropionic acid unit, 3-hydroxy-n-butyric acid unit, and 3-hydroxy-n-valeric acid unit.

In addition, an electrostatic charge image developing toner allows to design the toner characteristics such as chargeability, flowability, stability in time and environmental stability uniform among the toners of different colors. The toner has a small particle size enough for enabling uniform dispersion and being excellent in color saturation and transparency. The toner also shows higher contribution to the environmental security. The toner includes a coloring agent of which at least a part of the surface is covered with polyhydroxyalkanoate (PHA). The toner is produced by dispersing the coloring agent in aqueous medium, then fixing PHA synthesizing enzyme to the coloring agent dispersed in the aqueous medium, then adding 3-hydroxyacyl CoA, and executing a PHA synthesizing reaction to cover at least a part of the surface of the coloring agent with PHA. The toner thus obtained is used for an image forming method.

The invention discloses a preparation method and application of a composite microbial feed additive, belonging to the technical field of microbial engineering. The composite microbial feed additive provided in the invention comprises a live Clostridium butyricum preparation and a live Bacillus licheniformis preparation, wherein the live Clostridium butyricum preparation is obtained by the steps of seed culturing in triangular flasks, culturing in a seeding tank, culturing in a fermentation cylinder, bacterial sludge collecting by centrifugation, mixing with calcium carbonate, drying at low temperature, crushing, sieving and mixing with the accessory starch, and the live Bacillus licheniformis preparation is obtained by the steps of strain culturing on an inclined plane, culturing in eggplant-like flasks, culturing in a seeding tank, culturing in a fermentation cylinder, bacterial sludge collecting by centrifugation, mixing with calcium carbonate, drying at low temperature, crushing, sieving and mixing with the accessory starch. The composite microbial feed additive is obtained by evenly mixing the live Clostridium butyricum preparation and the live Bacillus licheniformis preparation according to a mass ratio of 1:1. The composite microbial feed additive can substantially raise the utilization rate of a feed, significantly reduce cultivation cost and increase economic benefits.