67 results about "Ethyl butyrate" patented technology

The invention relates to the technical field of industrial microorganisms, and particularly discloses a yeast strain and application thereof. The invention provides clavipora lusitaniae YJHT233, which is obtained through space mutation breeding, can tolerate 9% of alcohol, can tolerate an acid environment with the lowest pH value of 2.5, can tolerate a 12% high NaCl system, a 55% high glucose system and a 44 DEG C high temperature environment, and has excellent ester production capacity. The strain is used for brewing rice-flavor liquor or yellow wine, the total content of ester substances in the obtained product is greatly increased, the content of main volatile ester substances such as ethyl acetate, ethyl butyrate, isoamyl acetate, ethyl caprylate and ethyl lactate is remarkably increased, the whole flavor is coordinated and pleasant, and the fruit fragrance is rich and rich in layers.

To provide a novel technique that can improve a refreshing feeling and aftertaste of a lemon juice-containing beverage.SOLUTION: A lemon juice-containing beverage satisfies at least one of the following conditions: (A) fruit juice percentage: 4% or less, citric acid acidity: 0.4-1.0 g / 100 ml, and ethyl acetate content: 20-10000 ppb, (B) fruit juice percentage: 30% or less, citric acid acidity: 0.4-1.2, and ethyl butyrate content: 10-1000 ppb, (C) fruit juice percentage: 30% or less, citric acid acidity: 0.4-1.0, and 6-methyl-5-hepten-2-one content: 30-1000 ppb, and (D) fruit juice percentage: 4% or less, citric acid acidity: 0.4-1.2, and 1-octanol content: 40-1000 ppb.SELECTED DRAWING: None

The invention discloses red imported fire ant bait and a preparation method thereof. The invention relates to a red imported fire ant bait, which is prepared from the following components in percentage by mass: 56.76 percent to 61.99 percent of corn flour, 16.57 percent to 18.85 percent of dried meat floss, 16.50 percent to 18.90 percent of peanut oil, 3.08 percent to 4.22 percent of milk powder, 0.01 percent to 0.10 percent of ethyl butyrate, 1.34 percent to 1.70 percent of potassium sorbate and 0.01 percent to 0.50 percent of insecticide. Compared with the prior art, the solenopsis invicta bait has the following advantages that on the basis of background baits (corn flour, dried meat floss, peanut oil and milk powder), potassium sorbate keeps the freshness of the baits, a trace amount of ethyl butyrate is added, the appetite of solenopsis invicta is further stimulated, the palatability of the baits to the solenopsis invicta is improved, and the service life of the solenopsis invicta bait is prolonged. Particles with the average particle size of 1.5 mm are prepared through a pelletizer and are suitable for carrying of the solenopsis invicta, so that the attraction of the bait to the solenopsis invicta is further enhanced.

The invention discloses a diaphorina citri slow-release attractant and a preparation method thereof, the diaphorina citri slow-release attractant is composed of ethyl butyrate, myrcene, p-cymene, a paraffin carrier and cellulose acetate tows, and the ethyl butyrate, the myrcene, the p-cymene and the paraffin carrier are mixed and filled in the cellulose acetate tows. The diaphorina citri slow-release attractant provided by the invention has efficient attraction activity on diaphorina citri adults, the preparation method of the slow-release attractant is simple, low in cost, high in operability and suitable for industrial production, the oxidation and decomposition speed of active ingredients in ethyl butyrate, myrcene and cymene can be reduced by dissolving paraffin, and the effect of inducing diaphorina citri adults is achieved. The active ingredients are stably and continuously released for at least 20 weeks, and the composition is suitable for directly trapping and killing or monitoring diaphorina citri in the field.

The invention relates to the field of batteries, in particular to a battery. The battery comprises a diaphragm and an electrolyte, the diaphragm comprises a base material layer and a ceramic layer on at least one side surface of the base material layer, the ceramic layer comprises inorganic particles, the inorganic particles comprise an element Al, based on the total weight of the diaphragm, the content a of the element Al is greater than or equal to 12% and less than or equal to 32%; the electrolyte comprises ethyl butyrate, the content of the ethyl butyrate is c1 on the basis of the total weight of the electrolyte, and c1 is larger than or equal to 5% and smaller than or equal to 35%; and a and c1 satisfy 0.2 < = c1 / a < = 2.5. The battery provided by the invention can improve self discharge, and has excellent thermal stability and mechanical properties.

The invention provides hansenula polymorpha YC-C-16 capable of producing lactic acid and application of the hansenula polymorpha YC-C-16, and belongs to the technical field of microorganisms and wine brewing. The hansenula polymorpha YC-C-16 is characterized in that the hansenula polymorpha YC-C-16 is named as hanseniaspora uvarum in taxonomy, is preserved in the China Center for Type Culture Collection, has the preservation number of CTCC M 20252302, is preserved in Wuhan University on October 22, 2025, and is named as hanseniaspora uvarum in Wuhan University. The grape juice hansenula polymorpha YC-C-16 disclosed by the invention can be used for fermenting grape wine independently or together with saccharomyces cerevisiae, the content of lactic acid in the grape wine can be increased, and flavor substances such as ethyl butyrate, alpha-terpilenol, ethyl salicylate, ethyl myristate and trans-2-heptenal are added, so that the flavor level of the grape wine is increased. The hansenula polymorpha YC-C-16 provided by the invention can be widely applied to the field of wine fermentation.

According to the multi-solvent low-temperature high-voltage electrolyte for the supercapacitor, the method and the application, EA (ethyl acetate) and EB (ethyl butyrate) are added into a common solvent AN (acetonitrile) to serve as organic cosolvents, and the viscosity and the melting point of the electrolyte can be effectively reduced by mixing high-voltage-resistant and low-temperature-resistant imidazole ionic liquid; meanwhile, the stability of the electrolyte at high voltage is improved, the ionic conductivity of the electrolyte at low temperature is remarkably improved, and it is guaranteed that a supercapacitor using the electrolyte has excellent rate capability and cycling stability at the low temperature of-60 DEG C and the high voltage of 3.5 V.

The invention relates to the technical field of daily chemical essence, in particular to non-discoloring milk essence as well as a preparation method and application thereof. The invention relates to a non-discoloring milk essence, which is prepared from the following raw materials: 8 to 12 percent of ethyl myristate, 14 to 16 percent of delta-dodecalactone, 0.8 to 1.2 percent of ethyl butyrate, 1.5 to 2.5 percent of butyl butyryl lactate, 0.8 to 1.2 percent of furanone, 0.05 to 0.08 percent of oat beta-glucan, 0.3 to 0.6 percent of aloe polysaccharide, 0.8 to 1.2 percent of yeast extract, 0.39 to 0.65 percent of modifier, 6.16 to 7.08 percent of flavoring agent, 6.8 to 9.2 percent of fixative, 8 to 10 percent of antioxidant and the balance of solvent. The milk essence not prone to discoloration can meet the requirements of the market for white daily chemical products with high quality and good stability.

The invention provides litchi essence as well as a preparation method and a preparation device thereof. According to the technical scheme, the preparation method of the litchi essence comprises the following steps: S1, putting linalool, ethyl butyrate and other raw materials into the same container, and sealing and stirring to prepare first premixed essence; s2, putting geraniol, ethyl acetate and other raw materials into the same container, and sealing and stirring to prepare a second premixed essence; s3, adding trans-2-hexenal, gamma-decalactone and other raw materials into the same container, and sealing and stirring to prepare third premixed essence; s4, putting the first premixed essence and the second premixed essence into the same container, and sealing and stirring; and adding the third premixed essence, sealing and stirring to obtain the litchi essence. The lychee essence prepared by the invention has lasting and natural lychee fragrance, and has penetrating power and layering sense. In addition, the invention further provides a preparation device of the litchi essence.

The application discloses cherry essence, and a preparation method thereof. The cherry essence comprises the following components in parts by weight: 80-98 parts of propylene glycol; 0.5-3 parts of benzaldehyde; 0.5-3 parts of ethyl acetate; 0.1-1 part of acetic acid; 0.1-1 part of gamma-decalactone; 0.1-0.8 part of ethyl butyrate; 0.01-0.2 part of butyric acid; 0.005-0.03 part of isoamyl alcohol; and 0.005-0.03 part of butanol. The preparation method comprises the following steps: taking all components of the cherry essence according to the proportion requirements, stirring all components of the cherry essence in a stirring container for 10-15 minutes, and then storing the mixture in a storage container. The cherry essence can be used as a food additive, and can be used in various foods to enhance the natural cherry aroma and natural taste of the foods. The aroma quality and stability of the cherry essence are superior to those of the same kind of essence in China.

The invention relates to a method for preparing bio-oil and biochar from waste cotton, which comprises the following steps: by taking polar aprotic solvents including tetrahydrofuran, 4-methyltetrahydrofuran, ethyl acetate and ethyl butyrate as reaction solvents and sulfuric acid, phosphoric acid and the like as catalysts, synthesizing bio-oil and biochar from waste cotton under mild and low-energy-consumption conditions; the acid catalyst and the polar aprotic solvent effectively depolymerize cellulose in the waste cotton and catalyze pyrolysis of intermediate products to directionally dehydrate the intermediate products into high-value platform compounds such as levoglucosenone and furfural, and the solid-phase byproduct charcoal has a hierarchical porous structure and a high specific surface area and has a wide application prospect. The process provides a novel sustainable method for recycling, harmlessness and greenization of the waste cotton, and has both environmental benefits and economic benefits.

The invention relates to the technical field of cigarette perfuming, in particular to a cellulose core wire for cigarette perfuming and a preparation method of the cellulose core wire. The preparation method comprises the following steps: dipping a core wire in an essence solution, taking out the core wire after dipping, and drying to obtain the cellulose core wire for cigarette perfuming, the core yarn is made of pure viscose yarn with the linear density of 110-120 tex, the S twist and the twist degree of 60-110 twists per meter. In the essence solution, essence components comprise isoamyl isovalerate, ethyl butyrate and farnesol, and a solvent is ethanol. According to the preparation method, the stability and uniformity of the incense line in subsequent use are remarkably improved, and the consistency of essence loading and releasing is guaranteed; in addition, the prepared cellulose core wire has excellent performance and good on-machine applicability, and the situation that the cellulose core wire is seriously deformed or broken or the like is not prone to occurring in the process of being implanted into cigarette components.

The present invention provides a detection method for related substances of ethyl 4-bromobutyrate, and relates to the field of drug analysis. The method adopts a weak polar chromatographic column; nitrogen is used as a carrier gas, and the flow rate is 0.7-1.5 ml / min; injection is performed in split mode; a temperature control method of programmed heating is adopted, and the detector is a hydrogen flame ionization detector; gas chromatography is used to qualitatively or quantitatively detect related substances in ethyl 4-bromobutyrate. It has the characteristics of stable baseline, good peak shape, high separation, excellent specificity and system suitability, high sensitivity, and good durability, which makes up for the gap in the prior art, and is of great significance to the quality control of ethyl 4-bromobutyrate and the drug safety of elagolid sodium.

To suppress fading of food and drink colored with an anthocyanin-based pigment.SOLUTION: An effective amount of at least one kind selected from the group consisting of furaneol, vanillin, maltol, ethyl butyrate, hexyl acetate, trans-2-hexenal, isoamyl acetate, methyl hexanoate, ethyl 2-methyl butyrate, ethyl 3-hydroxyhexanoate, alpha-ionone and damascenone is added to food and drink colored with an anthocyanin-based pigment. In addition, a pigment preparation and a food and drink composition containing the same components are provided.SELECTED DRAWING: None

The present application relates to the technical field of liquor quality. The present application utilizes the same fungus Eurotium cristatum SXHBTBU1934 CGMCC NO.21026 as yeast to independently ferment wheat, and then utilizes the fermented wheat as "microbial filter material" to filter distilled liquor. The specific method for improving liquor quality by utilizing microbial fermentation material comprises the following steps: filtering liquor body by adsorption material to obtain filtered liquor body; mixing the filtered liquor body with washing-removing material to obtain washing-removed liquor body; and performing fine filtration on the washing-removed liquor body to obtain product liquor body. By utilizing the method of the present application, the compounds such as ethyl oleate and ethyl linoleate, which have negative influence on the flavor of liquor and have viscosity, can be reduced in the liquor body; meanwhile, the acid ester compounds such as ethyl octanoate are increased, which adds fruit flavor to the liquor body, but does not destroy the flavor balance of the four flavoring substances such as ethyl acetate, ethyl lactate, ethyl butyrate and ethyl hexanoate, and does not affect the main taste of the liquor body.

The invention discloses ester-synthesized clostridium aerovorans as well as a construction method and application thereof, and belongs to the technical field of genetically engineered bacterium, genetically engineered clostridium aerovorans capable of synthesizing ester is constructed, a clostridium Yangtinctorii engineering strain synthesized by butanol is taken as a host, and alcohol acyltransferase ATF1 gene from saccharomyces cerevisiae is over-expressed, so that the ester-synthesized clostridium aerovorans is obtained. The clostridium Yangtsangensis genetic engineering strain capable of producing various esters is obtained; 1.4 g / L of butyl acetate can be obtained from the genetic engineering strain in serum bottle fermentation; the fermentation yield can be further increased in a 3L fermentation tank, 12.5 g / L of butyl acetate is obtained, and meanwhile, a small amount of ester compounds such as ethyl acetate, ethyl butyrate and butyl butyrate are obtained; the genetic engineering clostridium aerovorans disclosed by the invention is suitable for fermenting green gases such as synthesis gas serving as raw materials to synthesize green butyl acetate.

The present invention relates to a flavor oil manufacturing method that involves: mixing a seasoning stock solution and an edible oil or fat to obtain a mixture; and fermenting the mixture. The present invention also relates to a flavor oil that satisfies at least one of (1) and (2) below. (1) A flavor oil containing a total of 2 ppb or more of at least two component selected from the group consisting of ethyl 2-methylpropanoate (component i), ethyl 2-methylbutanoate (component iii), and ethyl 3-methylbutanoate (component iv); (2) a flavor oil containing a total of 2 ppb or more of at least one component selected from the group consisting of ethyl 2-methylpropanoate (component i), ethyl 2-methylbutanoate (component iii), and ethyl 3-methylbutanoate (component iv), and containing 10 ppb or more of ethyl butanoate (component ii).

The application discloses a Pediococcus pentosaceus strain derived from high-temperature Daqu and application thereof, the strain is named Pediococcus pentosaceus zqw75, and the preservation number is CGMCC NO.25146.The strain can produce aroma substances such as lauryl acetate, n-heptanol, butyl dodecalactone, benzene acetaldehyde, methyl heptenone and pyrazine, and when the strain is used in combination with commercial sweet wine starter, the relative content of phenylethanol is increased from 8.562% to 10.455%, the relative content of ethyl butyrate is increased from 0.460% to 1.473%, the relative content of ethyl octanoate is increased from 0.754% to 1.672%, the relative content of ethyl n-hexanoate is increased from 0.832% to 3.446%, and the relative content of isoamyl acetate is increased from 0.832% to 2.155%; and the strain can produce aroma substances that are not produced by the commercial sweet wine starter, such as hexanoic acid, butyric acid, octanoic acid, ethyl hexanoate and lauryl acetate.The strain can be applied in the field of liquor making, including pit mud making and liquor flavor substance improvement, and has great industrial application value.

The present application relates to the technical field of brewing, and particularly provides a preparation method of cider with high ethyl butyrate content. The method is to brew cider by mixed fermentation of Saccharomyces cerevisiae and Torulaspora delbrueckii. The Torulaspora delbrueckii is preferably Torulaspora delbrueckii SN-6, which was preserved in the China General Microbiological Culture Collection Center on November 7, 2022, and the preservation number is CGMCC No. 26064. The total ester content and ethyl butyrate content of the cider prepared by the method are significantly improved, the total sugar content is significantly reduced, the taste and aroma quality are both good, and the market prospect is broad.

The invention relates to the technical field of compound synthesis, and provides a preparation method of 4-(4-hydroxymethyl-3-methoxyphenoxy) butyric acid, which comprises the following steps: S1, carrying out methylation reaction on a 2-phenolic hydroxyl group of 2, 4-dihydroxybenzaldehyde and a methylation reagent to generate 4-hydroxy-2-methoxybenzaldehyde; step S2, carrying out nucleophilic substitution reaction on the 4-hydroxy-2-methoxybenzaldehyde and ethyl bromobutyrate, so as to generate 4-(4-formyl-3-methoxyphenoxy) ethyl butyrate; step S3, enabling the ethyl 4-(4-formyl-3-methoxyphenoxy) butyrate to react with a reducing agent, so as to generate the ethyl 4-(4-hydroxymethyl-3-methoxyphenoxy) butyrate; and S4, carrying out a hydrolysis reaction on the ethyl 4-(4-hydroxymethyl-3-methoxyphenoxy) butyrate under an alkaline condition, so as to generate the 4-(4-hydroxymethyl-3-methoxyphenoxy) butyric acid. The preparation method of the 4-(4-hydroxymethyl-3-methoxyphenoxy) butyric acid is provided for the first time, and the method is high in safety and suitable for industrial production.

The present invention provides a Bacillus subtilis BS-TS1 and its application in preparing distiller's yeast and wine products, belonging to the technical field of brewing microorganisms. The deposit number of the Bacillus subtilis BS-TS1 provided by the present invention is CGMCC No.32285. The Bacillus subtilis BS-TS1 provided by the present invention has the advantages of high resistance to Penicillium and high production of aroma substances. The Daqu prepared using Bacillus subtilis BS-TS1 has significant anti-Penicillium properties and is not easily contaminated during the production process of Daqu. The lees juice prepared from the Daqu obtained by using Bacillus subtilis BS-TS1 is rich in volatile aroma substances, among which the content of acetoin reaches 150.944 mg / L, the content of pyrazine reaches 301.353 mg / L, and the content of 2,3-butanediol reaches 21.319 mg / L; in addition, other aroma components such as ethyl butyrate and phenylethanol are also detected.

The utility model discloses an esterification reaction kettle for producing R-4-cyano-3-hydroxy ethyl butyrate, which is characterized in that a motor is arranged in the center of the upper surface of a kettle cover through a motor seat, an output shaft of the motor is connected with a main shaft through a coupler, the bottom end of the main shaft is connected with a U-shaped stirring paddle, a jacket is wrapped outside a kettle body, heat exchange joints are arranged on two sides of the upper surface of the kettle cover, and the U-shaped stirring paddle is connected with a U-shaped stirring paddle. A spiral heat exchange tube is arranged on the lower surface of the kettle body, two ends of the spiral heat exchange tube are respectively connected with heat exchange joints, the spiral heat exchange tube is arranged outside the main shaft, the lower part of the spiral heat exchange tube is also positioned on the inner side of the U-shaped stirring paddle, the upper part of the spiral heat exchange tube is connected with a plurality of supporting pieces, and the outer ends of the supporting pieces are connected with the inner wall of the kettle body. When esterification reaction is carried out and heat preservation is finished, heat exchange between cooling water and the inside of the kettle body can be realized through the spiral heat exchange pipe in the kettle body, so that the temperature in the kettle is quickly reduced, the esterification efficiency is greatly improved, and the production efficiency of R-4-cyano-3-hydroxybutyric acid ethyl ester is improved.

Provided are: a food or drink containing glucosylceramide in which the odor of glucosylceramide is masked despite containing a relatively large amount thereof and a suitable flavor is maintained; and a method for improving the flavor of the food or drink. The food or drink contains glucosylceramide and at least one substance selected from the group consisting of isobutyl acetate, ethyl butyrate, isoamyl acetate, trans-2-hexenal, and benzaldehyde. The method for improving the flavor of the food or drink containing glucosylceramide includes a step in which at least one substance selected from the group consisting of isobutyl acetate, ethyl butyrate, isoamyl acetate, trans-2-hexenal, and benzaldehyde is added to the food or drink containing glucosylceramide.

A problem addressed by this invention is to provide a novel yogurt-flavored food having reduced oiliness.　A solution for solving said problem is a yogurt-flavored food that contains 20-80 mass% of oil / fat, 0.3-10 ppm of ethyl butyrate, 1-25 ppm of isoamyl acetate, and 2-50 ppm of limonene.

The invention relates to the technical field of pesticide preparation, and particularly discloses a novel preparation method of a key intermediate 1-(2, 4, 6-trichlorophenyl)-propyl-2-ketone of a bactericide pydiflumetofen. The method comprises the following steps: by taking 3, 5-dichloro-4-fluoronitrobenzene as a starting raw material, sequentially carrying out four-step unit reactions: reacting with ethyl acetoacetate under an alkaline condition to prepare 2-(2, 6-dichloro-4-nitrophenyl)-3-oxobutyric acid ethyl ester, and reacting with ethyl acetoacetate under an alkaline condition to prepare 2-(2, 6-dichloro-4-nitrophenyl)-3-oxobutyric acid ethyl ester; carrying out degreasing under an acidic condition, so as to obtain 1-(2, 6-dichloro-4-nitrophenyl)-propyl-2-ketone; reducing the nitro group to obtain 1-(4-amino-2, 6-dichlorophenyl)-propyl-2-ketone; and performing Sandmeyer reaction to obtain a target product. The preparation method is novel in synthetic route, few in side reaction and high in total yield, the content of a target product is larger than 99%, the preparation method is suitable for industrial production, and a key support is provided for industrialization of pydiflumetofen.

The application discloses a local high-concentration lithium metal battery electrolyte and a preparation method and application thereof. The electrolyte comprises a lithium salt, an organic solvent, an additive and a diluent; the organic solvent comprises at least one of methyl formate, methyl acetate, methyl propionate, methyl butyrate, ethyl propionate, ethyl acetate, ethyl butyrate, methyl trifluoroacetate and ethyl trifluoroacetate; the additive comprises at least one of fluoroethylene carbonate, lithium difluorobisoxalate phosphate and lithium difluorophosphate; and the diluent is a fluorine-containing ether compound. The local high-concentration electrolyte has the advantages of high-voltage resistance of high-concentration electrolyte and inhibition of corrosion of sulfonimide-based electrolyte on aluminum foil, meanwhile, the problems of high viscosity, low conductivity and poor impregnation with a separator of high-concentration electrolyte are overcome, the cycle performance and low-temperature performance of the lithium metal battery are significantly improved, and the local high-concentration electrolyte is particularly suitable for application in a low-temperature high-voltage lithium battery. The preparation method is simple, the cost is low, and the method is suitable for industrial production.

The invention discloses a drosophila okadaiensis imago attractant based on plant volatile matters and application thereof, and belongs to the field of insect control, the drosophila okadaiensis imago attractant comprises a female imago attractant: 0.2% of ethyl butyrate, 20% of n-hexyl alcohol and 20% of (2R, 3R)-(-)-2, 3-butanediol are mixed according to the volume ratio of 1: 1: 1; the male adult attractant is formed by mixing 20% of n-hexyl alcohol, 20% of (2R, 3R)-(-)-2, 3-butanediol and 0.2% of isoamyl alcohol according to the volume ratio of 1: 1: 1; the solvent is liquid paraffin. The botanical attractant which has high efficiency and species specificity for the vector and agricultural pest okadaioma drosophila is invented for the first time, and compared with a traditional attractant, the botanical attractant has the advantages that the trapping rate is increased, the botanical attractant has certain species specificity, and the botanical attractant has specific attraction capacity for sex. The method not only can be applied to pest control of the species in the field, but also has positive significance on dynamic monitoring and distribution of the vector insects, has an important effect on predicting the conjunctival sucking nematode disease propagated by the vector insects, and has a better application prospect.

The present invention relates to the technical field of lithium-ion batteries, and particularly relates to a non-aqueous electrolyte and a lithium-ion battery. The non-aqueous electrolyte includes a lithium salt and an organic solvent; in the non-aqueous electrolyte of the present invention, at least one carboxylic acid ester such as methyl propionate and ethyl butyrate is used as the organic solvent, two different lithium salts are adopted and one of them is lithium hexafluorophosphate, and the relationship among the mass percentage content a of the carboxylic acid ester in the non-aqueous electrolyte, the mass percentage content b of lithium hexafluorophosphate in the lithium salt, the total content n of the lithium salt in the non-aqueous electrolyte, and the thermal expansion rate d of the non-aqueous electrolyte satisfies 1.8 ≤ (10×d + n×b) / a ≤ 18, 10 ≤ a ≤ 60, 30 ≤ b ≤ 98, 0.8 ≤ n ≤ 1.5, and 3.2 ≤ d ≤ 10. When these conditions are met, the high-rate and high-safety characteristics of the battery can be balanced, the safety hazards of high-rate lithium battery products are reduced, and at the same time, it is beneficial to further improve the charging rate of existing batteries.

The invention provides bacillus subtilis BS-TS1 and application thereof in preparation of distiller's yeast and wine products, and belongs to the technical field of wine brewing microorganisms. The preservation number of the bacillus subtilis BS-TS1 provided by the invention is CGMCC (China General Microbiological Culture Collection Center) No. 32285. The bacillus subtilis BS-TS1 provided by the invention has the advantages of high penicillium resistance and high yield of aroma substances. The yeast for making hard liquor prepared by using the bacillus subtilis BS-TS1 has a remarkable penicillium resistance characteristic, and is not easily polluted in the making process of the yeast for making hard liquor. The vinasse juice prepared from the yeast prepared from the bacillus subtilis BS-TS1 is rich in volatile aroma substances, the content of acetoin reaches 150.944 mg / L, the content of pyrazine reaches 301.353 mg / L, and the content of 2, 3-butanediol reaches 21.319 mg / L; in addition, other aroma components such as ethyl butyrate, phenethyl alcohol and the like are also detected.

The present invention provides a beer-flavored beverage, which is characterized in that it contains 4-vinylguaiacol in an amount of 1000 mass ppb or less, the content of ethyl n-butyrate is 140 mass ppb or more, it contains at least one lactone selected from γ-decalactone and γ-undecalactone, and the ratio (ethyl n-butyrate / the lactones) of the content of ethyl n-butyrate (unit: mass ppb) to the total content of the lactones (unit: mass ppb) is 1100 or less.