The invention relates to an extraction method of 
mangrove plant DNA, comprising the following steps: (1) taking a leaf to 
grind, adding preheated 2% of CTAB extract to dissolve, and then adding 2 % of PVP by volume; (2) after water bath, adding a small quantity of RNase for heat preservation, then adding 
chloroform-isoamylol mixed liquor, and carrying out high-speed 
centrifugation at normal temperature; (3) putting supernate into a 
centrifuge tube, adding the preheated 2% of CTAB extract, adding 
chloroform-isoamylol mixed liquor, and mixing and carrying out high-speed 
centrifugation at normal temperature; (4) taking supernate and then putting into the 
centrifuge tube, adding NaCl solution and cold isopropanol, mixing, and putting and precipitating 
DNA at low temperature; (5) carrying out high-speed 
centrifugation at low temperature, abandoning the supernate, recovering the precipitate, washing by 
ethanol, air-
drying, adding 
TE buffer solution, and storing at low temperature. The invention can effectively remove 
polysaccharide and 
tannin produced in the 
DNA extraction process, and the obtained 
mangrove plant has high DNA content and high purity.