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643 results about "Isoamyl alcohol" patented technology

Isoamyl alcohol (also known as isopentyl alcohol) is a colorless liquid with the formula (CH₃)₂CHCH₂CH₂OH. It is one of several isomers of amyl alcohol. It is an ingredient in the production of banana oil, an ester found in nature and also produced as a flavouring in industry. It is a common fusel alcohol, produced as a major by-product of ethanol fermentation. It is also an ingredient of Kovac's reagent, used for the bacterial diagnostic indole test.

Preparation method and application of unsaturated polyether

The invention relates to a preparation method and application of unsaturated polyether. The preparation method comprises the following steps: (1) adding unsaturated alcohol and a catalyst into a first reactor, replacing with nitrogen, continuously adding epoxide with short branches and reacting to obtain an intermediate product A, wherein the unsaturated alcohol is one or a mixture of more than two of methyl allyl alcohol and isoamyl alcohol at any proportion and the mass ratio of the unsaturated alcohol to epoxide is 1.0:(0.1-5.0); and (2) putting the intermediate product A obtained in the step (1) and the catalyst into a second reactor, replacing with nitrogen, continuously introducing ethylene oxide and reacting to obtain polyether B, and neutralizing the pH value of the polyether B product to be 5-7 with a neutralizing reagent, wherein the mass ratio of the intermediate product A to ethylene oxide is 1.0:(15.0-51.0). The invention also provides the unsaturated polyether prepared by the preparation method, which is disclosed by the invention and application of unsaturated polyether in synthesis of polycarboxylic water reducer and the water reducer prepared from the unsaturated polyether synthesized by the method has the advantages of low doping amount, high water reduction rate, good slump-retaining agent and like.
Owner:HONGBAOLI GRP CO LTD

Essence particle additive as well as preparation method and application thereof

The invention discloses an essence particle additive as well as a preparation method and an application thereof. The particle additive consists of a particle carrier, cigarette essence and a polymeric membrane, wherein the particle carrier is used for loading the cigarette essence, and the polymeric membrane is used for coating the particle carrier loaded with the cigarette essence; the particle carrier is composed of calcium carbonate particles, a molecular sieve, tea dust, microcrystalline cellulose or cellulose microspheres; the cigarette essence is isoamyl alcohol, phenethyl alcohol, menthol, linalool, terpinol or limonene; and the polymeric membrane is prepared from sodium alginate tech grade, carboxymethyl cellulose, hydroxyethyl cellulose or pullulan. According to the physical form of the cigarette essence, the essence particle additive can be prepared by a liquid-state method and a solid-state method. When being applied to the flavoring of a cigarette filter and the production of a cigarette, the essence particle additive has the beneficial effects that when the cigarette is not sucked, the volatilization and diffusion of the essence are restrained, and when the cigarette is sucked, the essence can be uniformly released without the dependence on external forces.
Owner:CHINA TOBACCO CHUANYU IND

Ceramic product having alcoholic drink alcoholization function and preparation method thereof

The invention relates to an alcoholic drink storage utensil and a preparation method thereof. The alcoholic drink storage utensil is a ceramic product having an alcoholic drink alcoholization function and is composed of purple sand, an alcoholic drink purifying solvent, an energy activator and an environment-imitating solvent, wherein the purple sand accounts for 70-80% of the raw materials, the alcoholic drink purifying solvent accounts for 5-20% of the raw materials s, the energy activator accounts for 5-20% of the raw materials s, and the environment-imitating solvent accounts for 2-6% of the raw materials. The alcoholic drink purifying solvent contains major elements of potassium, sodium, calcium, magnesium and phosphorus and further contains zinc, iron, selenium, copper, strontium, iodine, fluorine and metasilicic acid; the energy activator can be tourmalinite or negative ion powder or a mixture of the tourmalinite and the negative ion powder; the environment-imitating solvent is diatomite. The function of the ceramic products is that parameters such as acetaldehyde, methanol, n-propanol, sec-butyl alcohol, isobutanol, n-butanol and isoamyl alcohol, and the like can be reduced to a different degree; parameters such as ethyl acetate, ethyl butyrate, ethyl lactate and ethyl caproate, and the like can be raised to different degrees. According to the invention, the alcoholic drink has good shade, virgin whiteness, no thickness, no pungent smell and no other off-flavors.
Owner:冯政

Method for preparing nanometer molybdenum disulfide by aid of microemulsion system

The invention provides a method for preparing nanometer molybdenum disulfide by the aid of a microemulsion system. The method includes steps of chemically reducing hexamolybdate, then adding sulfide into the hexamolybdate to perform sulfidation reaction so as to generate thiomolybdate sulfite; adding the thiomolybdate sulfite into TritonX100, isoamyl alcohol, normal heptane and secondary deionized water to form microemulsion a; adding hydrochloric acid into TritonX100, soamyl alcohol, normal heptane and secondary deionized water to form microemulsion b; adding the microemulsion b into the microemulsion a; fast mixing and stirring; leading acid sinking reaction to be thorough to obtain a product; and obtaining the nanometer molybdenum disulfide after the product is separated centrifugally, washed and dried in a vacuum manner. The preparation method is simple, reaction conditions are easy to be controlled, stability is good, generated particles are uniform, appearances are neat, and the nanometer molybdenum disulfide is expectably prepared on a large scale.
Owner:XI'AN UNIVERSITY OF ARCHITECTURE AND TECHNOLOGY

Extraction method of mangrove plant DNA

The invention relates to an extraction method of mangrove plant DNA, comprising the following steps: (1) taking a leaf to grind, adding preheated 2% of CTAB extract to dissolve, and then adding 2 % of PVP by volume; (2) after water bath, adding a small quantity of RNase for heat preservation, then adding chloroform-isoamylol mixed liquor, and carrying out high-speed centrifugation at normal temperature; (3) putting supernate into a centrifuge tube, adding the preheated 2% of CTAB extract, adding chloroform-isoamylol mixed liquor, and mixing and carrying out high-speed centrifugation at normal temperature; (4) taking supernate and then putting into the centrifuge tube, adding NaCl solution and cold isopropanol, mixing, and putting and precipitating DNA at low temperature; (5) carrying out high-speed centrifugation at low temperature, abandoning the supernate, recovering the precipitate, washing by ethanol, air-drying, adding TE buffer solution, and storing at low temperature. The invention can effectively remove polysaccharide and tannin produced in the DNA extraction process, and the obtained mangrove plant has high DNA content and high purity.
Owner:ZHEJIANG MARICULTURE RES INST

Mineral-floating foaming agent and its prepn

InactiveCN1403206AGood dispersionHigh foaming speedFlotationEpoxyFoaming agent
The present invention proposes one kind of mineral-floating foaming agent and its preparation. The foaming agent contains alcohol with branch chain and epoxy ethane and / or epoxy propane in the weight ratio of 1 to 1-6, and the alcohol with branched chain may be isopropyl alcohol, isobutyl alcohol or isoamyl alcohol. It is prepared through mixing alcohol with branched chain and epoxy ethane and / or epoxy propane, adding oxide of K or Na in 0.3-1% of material weight as catalyst, reaction at 0.01-1.2 MPa pressure and 170-190 deg.c temperature for 2-3 hr, decreasing the pressure to nomral pressure, cooling to below 50 deg.c and filtering. The foaming agent is water soluble, good in dispersivity, great in formed foam, favorable to secondary enrichment and recovery of fine mineral and has no environmental pollution.
Owner:WUHAN UNIV OF TECH

Method of producing a composite fermented beverage using genetically modified yeast strains

InactiveUS6326184B1Reduced lacking productionEasy to useFungiBeer fermentationThiolIsoamyl alcohol
Method of preparing a composite yeast fermented beverage such as beer including lager, with predetermined content of flavour compounds, comprising combining separate batches of beverage, of which at least one is a base beverage produced with a yeast strain having reduced or lacking production of one or more flavour compounds or flavour stabilizing compounds. In the method are used yeast strains including S. cerevisiae and S. carlsbergensis which have reduced or lacking production of sulphite, dimethylsulphide, thiols, thioesters, hydrogen sulphide, higher alcohols including isoamyl alcohol and / or alcohol esters.
Owner:CARLSBERG BREWERIES AS

Boron extraction technology

The invention discloses a boron extraction technology. Brine water after extracting lithium from salt lake is used as raw paderial and 2-thyl-1,3-hexanediol respectively with mixture alcohol of isooctal alcohol and isoamyl alcohol extracts borone from the brine water and optimum processing condition for extracting boron from brine water using the mixture alcohol is obtained after performing experiments from the aspects of extractant volume fraction, acidity, phase ratio, extraction time, extraction temperature, saturation extraction volume, back washing agent concentration and back washing time: extractant volume fraction 30%, water phase pH 3, phase ratio 1:1, extraction time 10min, maximum saturation capacity 61.4g / L(B2O3).
Owner:于网林

Formaldehyde scavenger and preparation method thereof

The invention belongs to the technical field of chemical preparations and particularly relates to a formaldehyde scavenger and a preparation method thereof. The formaldehyde scavenger comprises the following raw materials in parts by weight: 10-20 parts of amino acid, 10-20 parts of starch, 5-10 parts of casein, 5-10 parts of polysorbate, 10-15 parts of isoamyl alcohol, 30-50 parts of plant flower extracts, 5-10 parts of active carbon carboxymethylcellulose, 6-8 parts of a penetrant, 5-10 parts of pillared rectorite, 10-20 parts of chitosan and 80-120 parts of deionized water. The formaldehyde scavenger provided by the invention is high in absorption efficiency and capable of eliminating free formaldehyde in air, reducing the release amount of formaldehyde in a decorated product and simultaneously improving the stability of the formaldehyde in the decorated product so as to solve the problem of formaldehyde pollution caused after indoor decoration and the health of people is beneficial.
Owner:HEBEI UNIV OF ENG

Low Alcohol or Alcohol Free Fermented Malt Based Beverage and Method for Producing It

An alcohol free or low alcohol fermented malt based beverage is disclosed. The malt based beverage has an alcohol content of not more than 1.0 vol. % preferably not more than 0.7 vol. % having an aroma profile close to the one of alcoholic lager beers. The beverage has 7.00-30.00 ppm ethyl acetate and 0.01-0.20 ppm ethyl butyrate. The beverage preferably has the esters 0.05-2.00 ppm isoamyl acetate; 0.01-0.10 ppm ethyl butyrate; and 0.01-0.05 ppm ethyl hexanoate. The beverage preferably has the higher alcohol 5.00-30.00 ppm (iso-)amyl alcohol. The (iso)amyl alcohol is defined as the sum of 3-methyl butanol and 2-methyl butanol.
Owner:ANHEUSER BUSCH INBEV SA

Efficient neutral formaldehyde capture agent and preparation method

The invention provides efficient neutral formaldehyde capture agent, which consists of deionized water, melamine, formalin solution, methyl salicylate, isoamyl alcohol, polysorbate, diethyl phosphate, sodium dodecyl benzene sulfonate and activated carbon. The invention further provides a method for preparing the efficient neutral formaldehyde capture agent. The efficient neutral formaldehyde capture agent can rapidly react with formaldehyde to form innocuous and unpoisonous colorless transparent liquid with excellent stability, and can not produce secondary pollution for environment at the same time. The efficient neutral formaldehyde capture agent is low in cost of raw materials, simple and easy in production process, and simple and convenient to use.
Owner:WENZHOU WINTOP BIOLOGICAL TECH

Method for extracting high-molecular-weight genome from animal feces

The invention relates to a method for extracting high-molecular-weight genome from animal feces. The method comprises the steps of sample treatment, bacterial cell lysis and extraction of DNA (Deoxyribonucleic Acid). Specifically, the method comprises the steps of: dissolving an animal feces sample with aseptic PBS (Phosphate Buffered Saline) buffer, carrying out low-speed centrifuging and filtering to remove food residues in the feces, collecting bacterial cells, performing wall breaking on the bacteria by adopting lysozyme, decolorizing peptidase, protease and SDS (Sodium Dodecyl Sulfate), extracting with a mixture of phenol, chloroform and isoamyl alcohol as well as a mixture of chloroform and isoamyl alcohol, precipitating with isopropanol and sodium acetate, washing and drying, dissolving, and digesting RNA (Ribonucleic Acid) with RNAse (Ribonuclease). According to the invention, the method is simple in operation; and the DNA molecular weight of the extracted genome achieves more than 40kb, so that the researches on microorganism molecule ecological diversity, molecular identification, target gene amplification, macro genome library construction and the like are met.
Owner:YUNNAN NORMAL UNIV

High-yielding ethanol yeast and method for improving quality of traditional fermented foods through symbiotic fermentation of high-yielding ethanol yeast and ester-producing yeast

ActiveCN109810910AIncreased ethanol productionOptimizing the Culture Conditions of Symbiotic FermentationFungiAlcoholic beverage preparationHydrolysateEthyl acetate
The invention relates to a saccharomyces cerevisiae Y3401 strain for high-yielding ethanol and a symbiotic fermentation culture method of the saccharomyces cerevisiae Y3401 strain and a Wickerhamomyces anomalus Y3604 strain for high-yielding ethyl acetate as well as application of the saccharomyces cerevisiae Y3401 strain. The saccharomyces cerevisiae Y3401 was preserved in China General Microbiological Culture Collection Center on October 20th, 2017, with a preservation number of CGMCC No.14828; the similarity of 26S rDNA D1 / D2 sequence of the saccharomyces cerevisiae Y3401 strain to 26S rDNA D1 / D2 sequence of other saccharomyces cerevisiae strains; symbiotic fermentation of two yeast strains in a sorghum enzymatic hydrolysate culture medium by a standing or shaking mode has the advantages that the content of the ethyl acetate is favorably improved and the contents of flavor substances such as total ester, beta-phenylethanol and isoamyl alcohol also can be improved. In a solid brewing system, the symbiotic fermentation of the two strains can enhance the characteristics of ester-improving and flavor-enhancing of yeast. The symbiotic fermentation method of the two strains, disclosed by the invention, can be applied to the brewing industries, having demands on the ethyl acetate, such as Baijiu, yellow rice wine and soy sauce.
Owner:BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY

Maple flavor formula

ActiveCN103704659AReduce harsh chemical smellIncrease burnt aromaFood ingredient as flavour affecting agentFood preparationFood additiveAdditive ingredient
The invention discloses a maple flavor formula, which is mainly composed of ethyl maltol, methyl cyclopentenolone, ethyl butyrate, furanone, vanillin, a caramel reactant, maltol, 2,3,5-trimethyl pyrazine, isoamyl alcohol and guaiacol. The maple flavor prepared by the formula has obvious advantages that caramel reactant obtained from natural reaction is used as the key material, and a matching formula is employed for preparation of the maple flavor. As a food additive used in food, the maple flavor can endow a product with a maple aroma and taste with natural sense; at the same time, the added natural caramel reactant reduces stiff chemical smell of a single aroma material for the product and increases maple taste, so that the quality and quantity of aroma are higher than those of the similar domestic essence; and the heat resistance and stability of the product are also higher than those of the similar domestic product.
Owner:HANGZHOU EVER MAPLE FLAVOR & FRAGRANCE

Additive for improving resisting phase separation performance and heat value of methanol gasoline

The invention discloses an additive for improving resisting phase separation performance and a heat value of methanol gasoline. The additive consists of the following raw materials in percentage by weight: 35-45 percent of aliphatic alcohol, 10-20 percent of aliphatic ether, 15-30 percent of aliphatic ester, 5-15 percent of aliphatic acid, 1-5 percent of aliphatic amine and 0.1-3 percent of emulsifier OP, wherein the aliphatic alcohol is one or more of isopropyl alcohol, n-propanol, n-amyl alcohol, isoamyl alcohol and n-butyl alcohol; the aliphatic ether is one or more of ethylene glycol methyl ether, ethylene glycol ethyl ether and ethylene glycol butyl ether; the aliphatic ester is one or more of butyl acetate, n-amyl acetate and isoamyl acetate; the aliphatic acid is oleic acid and / or naphthenic acid; the aliphatic amine is dimethylamine and / or diethylamine; and the emulsifier OP is emulsifier OP-15 and / or emulsifier OP-20. The methanol gasoline in which the additive is added has superior low-temperature resisting phase separation and water resisting phase separation performances and an obviously improved heat value.
Owner:西安祺祥能源科技发展有限公司

Method for detecting lncRNA (long noncoding RNA) in plasma

The invention discloses a method for detecting lncRNA in plasma. Through steps of TrizolLS pretreatment of theplasma, chloroform and isoamyl alcohol mixed liquid extraction, isopropanol precipitation, ethanol washing, RNA RT (reverse transcription), PCR (polymerase chain reaction) detection and the like, a TrizolLSreagentcan well remove impurities such as polysaccharide, mucoprotein and the like in blood, RNA degradation is less, the phenomenon thattrichloromethane is easy to emulsify under the TrizolLS strong acid condition is effectively eliminated by a chloroform and isoamyl alcohol mixed liquid, high-quality and high-content extraction of RNA in the plasma is realized, fluorescence quantitative RT-PCR detection is performed on the quality of lncRNA, and meanwhile, a reference Ct (cycle threshold) value of the total RNA (that is, cDNA) mass can be detected.
Owner:NINGBO UNIV

Extracting method of polysaccharide and polyphenol plant genomes

The invention belongs to the technical field of molecular biology and discloses an extracting method of polysaccharide and polyphenol plant genomes. The method includes: grinding plant materials, then adding a nucleic acid separation buffer solution and 2-mercaptoethanol, well mixing, and placing into water bath; centrifuging in a centrifuge, and then discarding supernate; adding a 1x PBS solution, well mixing on a grinding instrument in an oscillation manner, and discarding supernate after centrifuging; adding preheated 3x CTAB lysate into sediment, well mixing, and performing splitting decomposition under water bath; adding equal-volume phenol / chloroform / isoamyl alcohol mixed liquor, well mixing and centrifuging; taking supernate, adding equal-volume chloroform / isoamyl alcohol mixed liquor, well mixing and centrifuging; taking supernate, adding NaCl and icy isopropanol, well mixing, and precipitating for 1-3 hours; taking out and centrifuging, then washing sediment with ethanol, and adding TE for dissolving after air drying so as to complete the extracting process. The extracting method has the advantages that polysaccharides and polyphenols can be removed effectively, the influence of the polysaccharides and polyphenols on nucleic acid extraction is reduced, and DNA quality and concentration are increased.
Owner:SHANGHAI PASSION BIOTECHNOLOGY CO LTD

Low-yield high-grade alcohol high-yield ethyl lactate saccharomyces cerevisiae strain and construction method thereof

InactiveCN105969678AReduce productionOvercome flavor incongruityFungiHydrolasesHeterologousAlcohol production
The invention relates to a low-yield high-grade alcohol high-yield ethyl lactate saccharomyces cerevisiae strain and a construction method thereof. The method comprises acquisition of a target gene, construction of a chromosome integrated vector and fermented verification of the breeding bacterial strain. The selected bacterial strain performs heterogenous secrete expression on pseudonym antarctica lipase B, compared with parent strains, the fermentation performance of the constructed saccharomyces cerevisiae is not influenced, after fermentation by sorghum raw material semi-solid liquor for 6 days, ethyl lactate content of the breeding bacterial strain is increased by 57.4%-84.5%; the high-grade alcohol production amount is obviously reduced, isoamyl alcohol is reduced by 9.5%, isobutyl alcohol is reduced by about 50.0%, and active-amyl alcohol is reduced by about 44.0%. According to the breeding bacterial strain, the production amount of ethyl lactate is obviously increased, the high-grade alcohol production amount is obviously reduced, the problem of incompatibility of flavor of the wind can be solved at certain degree, and the method has wide application prospect in the wine-related filed.
Owner:TIANJIN UNIV OF SCI & TECH

Extraction method of total genome DNA from microbes

The invention discloses an extraction method of total genome DNA from microbes, comprising the following steps of: adding a lysis buffer and lysozyme to a sample to be extracted to enable bacterial cells to fully rupture and release DNA; and extracting the obtained crude extract DNA by phenol / chloroform / isoamyl alcohol extracting solutions to obtain purified total genome DNA of microbes. The extraction method disclosed by the invention has the beneficial effects of big DNA release amount, simple experiment conditions, low cost and the like.
Owner:EAST CHINA NORMAL UNIVERSITY

Clean diesel fuel additive

The invention relates to an additive of clean diesel oil, which is characterized in that the additive of the clean diesel oil is mixed proportionately by the mixture of negative ion surface active agent, the mixture of nonionic surface active agent and isoamyl alcohol. Carbon atom which is less than or equal to 6 and more than or equal to 3 of a linear chain or a branched chain of alcohol is added to the mixture to serve as cosurfactant and the addition quantity is 3 to 5 percent of total amount of the mixture. The components of the clean diesel oil are that: 60 percent of diesel oil and 40 percent of clean diesel oil additive which are mixed into the clean diesel oil in the temperature of 20 DEG C to 25 DEG C. The invention is a clean diesel oil technique with low cost and the clean diesel oil additive and the mixture of the clean diesel oil and the diesel oil can reach each index of lightweight diesel oil, thereby the invention can be used in equipments of a diesel engine, an oil burning boiler, etc., and the fume smoke intensity FSM, particulate matter PM10, nitrogen oxides NOX, carbon monoxide CO, carbon dioxide CO2, sulphide SX, etc., of discharged harmful substances can be reduced, thus greatly improving air environment and having remarkable economic and social benefits.
Owner:鞍山市佳良能源技术开发有限公司

Separation and refining method of 1,3-methyl glycol in ferment liquor

This invention relates to a method for separating and purifying 1,3-dihydroxypropane from microbial fermentation liquor, comprising: after the sterilization and dehydration of fermentation liquor, add in 1-pentanol or isoamyl alcohol, n-butanol and n-hexanol, then remove the sediment in the fermentation liquor by filtration or settlement followed by washing; add in appropriate amount of glycerol or 1,4-butylene glycol to the supernatant to recover 1-pentanol or isoamyl alcohol, n-butanol and n-hexanol, by decompression or normal pressure distillation, so as to produce high-purity 1,3-dihydroxypropane, with more than 95% recovering rate. This invention is characterized of simple separation process, mature technique, low separation cost, and little additional introduced agent with easy recovery. Besides, the crystallization sediment in the fermentation liquor can be efficiently separated, which overcomes the problems of foaming, agglomerating and pipe clogging during distillation, so as to realize continuous operation.
Owner:PETROCHINA CO LTD

A method for extracting microbial total rna in forest soil and litter

The invention discloses a method for extracting microbial total RNA in forest soil and litter, which comprises the following steps: 1, carrying out freeze-drying on a forest soil or litter sample, grinding, and uniformly mixing; 2, adding pyrocarbonic acid diethyl ester treating water into the powdered sample, and standing over night at -70 DEG C; 3, adding glass beads, hexadecyl trimethyl ammonium bromide buffer, lauryl sodium sulfate, diatomite and phenol / chloroform / isoamyl alcohol, and uniformly mixing; 4, severely shaking the mixed liquid, and centrifuging to obtain the supernate; 5, adding guanidinium isothiocyanate into the supernate, and centrifuging to obtain the supernate; 6, adding chloroform / isoamyl alcohol into the supernate, and centrifuging to obtain the supernate; 7, addingpolyethylene glycol 6000 into the supernate, standing, centrifuging, and removing the supernate; 8, washing, dissolving, and carrying out DNA enzyme digestion to obtain an RNA solution; and 9, detecting the integrality of the RNA through agarose gel electrophoresis, and using a nucleic acid protein determinator to detect the concentration and purity of the RNA. The RNA extracted by using the method has high yield, good integrality and better purity.
Owner:INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI

Extraction method of total DNA of microorganisms from soil sample

The invention relates to an extraction method of total DNA of microorganisms from a soil sample. The extraction method particularly includes following steps: (1) adding a DNA extraction buffer solution to the soil sample and performing repetitive freeze-thawing in liquid nitrogen and hot water bath; (2) adding a protease K, performing a water bath treatment process at 37 DEG C for 30 min; (3) adding a sodium dodecyl solution, performing a water bath treatment process at 65 DEG C for 30 min; (4) performing centrifugation to remove precipitations and collect a supernate; (5) performing extraction with a mixed solution containing phenol, chloroform and isoamylol and performing centrifugation to collect a supernate; (6) performing extraction with a mixed solution containing chloroform and isoamylol and performing centrifugation to collect a supernate; (7) adding isopropanol to the supernate in the step (6) to performing precipitation and collecting a precipitation after centrifugation; (8) adding ethanol for washing the precipitation and drying the precipitation at the room temperature; (9) adding a TE buffer solution for dissolving the precipitation to obtain a DNA crude extraction; and (10) purifying the DNA crude extraction through an adsorption column, packaging the purified DNA crude extraction and storing the purified DNA crude extraction at -20 DEG C. The extraction method is especially effective when being used in samples from deep soil and dry sand which are less in biomass and in a clayed soil sample. The DNA extraction can be directly used in subsequent molecular biological detection.
Owner:CHINA PETROLEUM & CHEM CORP +1

Composite additive agent for reducing methanol gasoline saturated vapour pressure

The invention provides a composite additive for reducing the saturated vapor pressure of methanol gasoline. Ethanol, allyl alcohol, toluene, nitromethane, cyclohexanone, ethylenediamine, xylene, 1,2-propylene glycol, isoamyl alcohol and the like are evenly mixed at normal temperature and normal pressure to prepare the composite additive. The composite additive is mixed with industrial methanol to produce denatured alcohol, and the denatured alcohol is mixed with base oil to produce finished products of the methanol gasoline. The composite additive has the advantages of effectively reducing the saturated vapor pressure of the methanol gasoline and eliminating the high-temperature air resistance phenomenon during the use of the methanol gasoline.
Owner:山西华顿实业有限公司

Production method of N-ethyl iso penlyl amine and its device

A process for preparing the N-ethyl isopentylamine includes such steps as proportionally mixing isopentanol with ethylamine, loading the liquid mixture along with carried catalyst and H2 into vaporizing chamber, pre-heating for vaporizing, catalytic ammonolyzing reaction in fixed-bed reactor, condensing the resultant, and rectifying.
Owner:ZHEJIANG JIANYE CHEM

Preparation method for isopentyl aldehyde

The invention relates to a process for preparing isopentyl aldehyde by using isoamyl alcohol as initial raw material, which comprises, heating isoamyl alcohol to evaporation, using brass category catalyst for high temperature catalytic dehydrogenation reaction, condensing and disintegrating to obtain the isoperntyl aldehyde product. The said brass category catalyst is copper / zinc alloy, copper / tin alloy or copper / nickel alloy.
Owner:SHANDONG NHU PHARMA +1

Assay kit and method for simultaneously extracting and purifying ribose nucleic acid (RNA) and deoxyribose nucleic acid (DNA)

InactiveCN102911932AThe extraction efficiency is quiteDNA preparationSodium acetateNucleic acid detection
The invention discloses an assay kit and a method for simultaneously extracting and purifying ribose nucleic acid (RNA) and deoxyribose nucleic acid (DNA). The assay kit comprises extraction lysis buffer, 2M sodium acetate, phenol water, chloroform / isoamyl alcohol mixed liquor, isopropyl alcohol, 70% or 75% diethylpyrocarbonate (DEPC) alcohol, sterilized DEPC water, back extract and a nucleic acid purification column. The assay kit and the method have the advantages that the method for simultaneously extracting RNA and DNA is built and the assay kit is assembled. Compared with the classical separate extraction method which separately extracts RNA or DNA, the method has the comparative extraction efficiency, and moreover, the method also provides a method for further purifying RNA and DNA. The method has the advantages of simultaneously extracting and purifying RNA and DNA, and users can select different methods to extract nucleic acids according to the practical detection requirements. In addition, the assay kit and the method also provide a good basic platform for a detection technology for detecting two different types of nucleic acids.
Owner:PEOPLES REPUBLIC OF CHINA BEIJING ENTRY EXIT INSPECTION & QUARANTINE BUREAU

Low-yield higher-alcohol saccharomyces cerevisiae engineering bacterium and construction method thereof

The invention discloses low-yield high-alcohol saccharomyces cerevisiae engineering bacterium and a construction method thereof. The saccharomyces cerevisiae AY-20 engineering bacterium has the preservation number of CGMCC No.3930. Under the condition that other fermenting performances are not influenced, the content of the converted sub strain is respectively reduced by 55.19 percent and 34.43 percent compared with isobutanol and isoamyl alcohol of a parent strain, and the content of total higher alcohol is reduced by 35.01 percent. The invention can be realized by inserting a nucleotide sequence into start bacterium of saccharomyces cerevisiae 2.1190 or deleting partial or complete amino acid transaminase coding gene (BAT2). If the BAT2 is deleted by 100 percent, the back mutation is not easy to generate and a KanMX resistance gene in a recombinant strain is eliminated so as to ensure the safety of the strain and fermenting products. The screened engineering bacterium has no special requirements on the fermenting equipments and conditions and can be used by equipments and conditions in a general wine factory, so the invention has wide application potentials and can bring remarkable economic benefit for industrial production of a while spirit factory.
Owner:TIANJIN UNIV OF SCI & TECH

Method for extracting vanadium from vanadium-precipitated wastewater through microemulsion

The invention discloses a method for extracting vanadium from vanadium-precipitated wastewater through microemulsion. The method comprises the following steps: collecting vanadium-precipitated wastewater, and regulating the pH of the vanadium-precipitated wastewater to be 6.0-7.0; adding quaternary ammonium salt N236 and isoamyl alcohol to n-heptane, and stirring to uniformly mix to obtain an organic phase; slowly dropwise adding a mixed solution of NaOH and NaCl to the organic phase; stirring to uniformly mix; standing, wherein the mixed solution is subjected to phase separation after being clarified; collecting the upper phase to obtain saturated W / O microemulsion; mixing the W / O microemulsion and the vanadium-precipitated wastewater, and stirring for a certain time; then standing to layer the mixed solution; collecting the lower water phase; detecting the concentration of vanadium; and calculating the concentration of the organic-phase vanadium through a material balance method, thus calculating the extracting rate. With the adoption of the method, vanadium element in the vanadium-precipitated wastewater can be efficiently and quickly recovered.
Owner:CHONGQING UNIV
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