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940 results about "Isothiocyanate" patented technology

Isothiocyanate is the chemical group –N=C=S, formed by substituting the oxygen in the isocyanate group with a sulfur. Many natural isothiocyanates from plants are produced by enzymatic conversion of metabolites called glucosinolates. These natural isothiocyanates, such as allyl isothiocyanate, are also known as mustard oils. An artificial isothiocyanate, phenyl isothiocyanate, is used for amino acid sequencing in the Edman degradation.

Fluorescence quantitative PCR detection kit of hepatitis B virus and application thereof

The invention discloses a fluorescence quantitative PCR detection kit of hepatitis B virus and an application thereof. The kit is composed of the following independent components: DNA extraction solution I, DNA extraction solution II, DNA extraction solution III, DNA extraction solution IV, positive control interior label, PCR reaction liquid, probe HBV-SP, enzyme mixed liquor containing heat resistant DNA polyase and uracil DNA glycosylase, quantitative hepatitis B virus reference material, hepatitis B virus positive control serum and hepatitis B virus negative control serum, wherein DNA extraction solution I contains 0.2-1.0% of lauryl sodium sulphate (mass/volume), 1.0-4.0% of Triton (volume/volume) and 0.2-1.0mol/L of guanidinium isothiocyanate; DNA extraction solution II contains 100-300mmol/L of 4-HEPES, 100-300mmol/L of sodium chloride with pH of 6.5+/-0.2 and 100-400 mu g/ml of magnetic beads; DNA extraction solution III contains 0.1-1.0% of Triton (volume/volume) and 100-300mmol/L of sodium chloride; DNA extraction solution IV contains mineral oil. The fluorescence quantitative PCR detection kit of hepatitis B virus of the invention can be used for detecting the HBV-DNA concentration in samples of serum, blood plasma or latex and the like.
Owner:SANSURE BIOTECH

Method for extracting total RNA from plant tissue rich in polysaccharides and polyphenols and secondary metabolites

The invention provides an extration method of plant RNA by utilizing guanidinium isothiocyanate-chloroform extractive and Tris purification. The invention improves traditional guanidinium isothiocyanate method, adopts the two steps of chloroform extration after cracking and phenol-adding for purification, and respectively extract high-quality total RNA from the leaf blade and seed case of red-skinned pears, the leaf blade, fruit flesh and seed case tissues of grapes, the fruit of strawberries and the callus of arnebia euchroma. The extraction buffer liquid in the method contains guanidinium isothiocyanate, sodium dodecyl sarcosinate, sodium citrate, soluble polyvinylpyrrolidone and beta-mercaptoethanol, which can not only crack plant cells efficiently and release RNA, but also effectivelyinhibit the activity of RNA enzyme, and can also prevent the oxidation of phenolic compounds and suppress the interference of secondary metabolites. In the second step, Tris purification is utilized for crude extraction of RNA solution, which can eliminate the polysaccharides and protein which can coprecipitate with RNA. The invention is simple in operating steps, economic and practical, good in stability, and suitable for extracting high-quality total RNA from plant tissue rich in polysaccharides and polyphenols and secondary metabolites.
Owner:KUNMING UNIV OF SCI & TECH
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