148 results about "Pepsin" patented technology

The invention relates to the field of polypeptides, in particular to a red camellia polypeptide as well as a preparation method and application thereof. Red camellia is used as a raw material, high-pressure homogenization wall breaking and pepsase enzymolysis are performed, and separation and purification are performed to obtain the red camellia polypeptide with repairing, moisturizing, oxidation resisting and nourishing functions. The polypeptide prepared by the invention is more efficient, safer and more environment-friendly, and has a wide application prospect in the fields of cosmetics, functional foods and biological medicines.

The invention discloses a preparation method of micromolecular active collagen peptide, which comprises the following steps: (1) cutting fresh leather into fragments, pretreating the leather with a sodium bicarbonate solution, rinsing the leather with deionized water, and refrigerating and storing; (2) mixing papain, bromelain, pancreatin, pepsase, alkaline protease, neutral protease and flavourzyme to obtain a compound enzyme preparation; (3) performing high-pressure steam sterilization on the leather at 110-140 kPa and 120-122 DEG C to obtain leather with the hydrolysis degree of 75-85%, then adding distilled water into the leather to obtain mucilage, then adding a compound enzyme preparation into the mucilage, reacting for 5.5-6.5 hours at the pH of 6.8-7.2 and the temperature of 50-55 DEG C, and then performing enzyme inactivation at the temperature of 85-95 DEG C; and (4) after the reaction system is cooled to room temperature, purifying and centrifuging the reaction system, and taking clarified supernate, thereby obtaining the product. Different animal skins are treated, the proportion of peptide fragments with the molecular weight smaller than 1000 Da of the prepared small-molecule active collagen peptide is larger than or equal to 80%, and the hydroxyproline content is larger than 3%.

The invention provides a pepsinogen II determination kit and a quantitative determination method, the kit comprises a reagent R1 and a reagent R2, the reagent R1 comprises a luminous donor molecule labeled pepsinogen II first antibody and a reaction buffer solution; and the R2 reagent comprises a light-emitting receptor molecule labeled pepsinogen II second antibody and a reaction buffer solution. A double-antibody sandwich structure marked by a luminescent donor and a luminescent receptor respectively is adopted, binding and luminescence can be completed in a liquid phase, solid phase coating and subsequent multi-step washing are not needed, real homogeneous detection and one-step incubation are achieved, the operation process is simplified, the detection time is shortened, and a full-automatic platform is adapted; and an improved buffer system can inhibit non-specific adsorption and reduce heterotropism interference such as RF / HAMA, so that the detection accuracy and repeatability are improved.

The invention discloses a casein-sourced multifunctional peptide, and belongs to the field of food science and nutriology. The casein is subjected to enzymolysis through pepsin, a method combining activity verification and mass spectrum identification is adopted, the novel multifunctional peptide YPE with a clear sequence is screened and verified, the highest ABTS clearance rate of the casein peptide YPE can reach 77.76%, the highest hydroxyl radical clearance rate of the casein peptide YPE can reach 40.18%, and the casein peptide YPE can be used for preparing the novel multifunctional peptide YPE. The inhibition rate of alpha-glucosidase reaches 30.47%, the inhibition rate of escherichia coli is 10.18%, and the limitation of an existing single-function peptide in application is overcome.

The invention relates to a chemiluminescence kit for detecting pepsinogen II. The kit comprises an enzyme marker, a luminescent substrate solution and an anti-interference buffer system, the anti-interference buffer system comprises a nonionic surfactant, animal-derived IgG (immunoglobulin G), an antioxidant and catalase; the chemiluminescence kit also comprises a PG II calibration product and a PG II quality control product. The core active component of the enzyme marker is beta-galactosidase. The enzyme marker further comprises an anti-PG II monoclonal antibody marked by beta-galactosidase, bovine serum albumin, trehalose, cane sugar, MgCl2. 6H2O, sodium chloride, Proclin300 and a phosphate buffer solution. According to the kit, a brand-new anti-interference buffer system is introduced on the basis of a spatial proximity chemiluminescence analysis method, so that the resistance to clinically common interferents (such as hemolysis, lipemia, jaundice and heterotropism antibodies) is remarkably improved, and the detection result is more accurate and reliable.

The invention discloses a preparation method of a fructus cannabis protein peptide product with effects of resisting fatigue and improving exercise ability, which comprises the following steps: performing preliminary crushing, ultralow-temperature freezing micro-crushing and low-temperature continuous phase-change extraction degreasing on fructus cannabis, performing step-by-step hydrolysis by using polysaccharase and 2.4 L bacterial protease to extract fructus cannabis protein, performing low-temperature salting-out, and performing freeze-drying to obtain the fructus cannabis protein peptide product with the effects of resisting fatigue and improving exercise ability. The fructus cannabis protein peptide powder is prepared through low-temperature ultrasonic dissolution promotion, high-pressure micro-jet homogenization, compound enzyme C hydrolysis, 1% pepsase, high-temperature enzyme deactivation, chlorophyll removal through counter-current extraction, hydrogen peroxide decoloration, ultralow-temperature instantaneous concentration, low-temperature vacuum belt type drying and low-temperature ultrahigh-pressure sterilization. The fructus cannabis protein peptide prepared by the invention has the following remarkable effects: 1, the exercise ability is remarkably improved, the accumulation of lactic acid and urea nitrogen is reduced, the exhaustion time is prolonged, and the physical and mental fatigue is relieved; 2, the content of hepatic glycogen and muscle glycogen is obviously increased, the energy reserve of the body is increased, the explosive power and the reactivity are improved, and the athletic performance is improved; and 3, the antioxidant level of the body is improved, and fatigue and immune recovery after exercise are promoted.

The invention provides a PGI monoclonal antibody combination for pepsinogen I detection and application thereof, the PGI monoclonal antibody combination for pepsinogen I detection comprises a first antibody and a second antibody, the first antibody comprises a first light chain variable region and a first heavy chain variable region, and the second antibody comprises a second light chain variable region and a second heavy chain variable region. The second antibody comprises a second light chain variable region and a second heavy chain variable region; wherein the amino acid sequence of the first light chain variable region comprises VL-CDR1-3 as shown in SEQ ID NO: 1-3; the amino acid sequence of the first heavy chain variable region comprises VH-CDR1-3 as shown in SEQ ID NO: 4-6; the amino acid sequence of the second light chain variable region comprises VL-CDR1-3 as shown in SEQ ID NO: 7-9; and the amino acid sequence of the second heavy chain variable region comprises VH-CDR1-3 as shown in SEQ ID NO: 10-12. The monoclonal antibody combination can improve the sensitivity and accuracy of in-vitro detection.

The invention discloses a sea-buckthorn small molecule peptide and a preparation method thereof, and relates to the field of biological medicine and food processing. According to the method, sea-buckthorn pulp residues and sea-buckthorn seed residues are used as composite raw materials and are mixed and stirred to form homogenate, and then cell wall structures are destroyed through ultrasonic wall breaking treatment to release protein components; then cutting off a peptide bond under the action of compound enzymes (plant protease, neutral protease and pepsase) by adopting an ultrasonic-enzyme synergistic hydrolysis process to generate a small molecule peptide fragment; after removing impurities through centrifugal separation, purifying through an ultrafiltration membrane (the molecular weight cut-off is 1-3kDa) to obtain a small molecule peptide solution; and performing debitterizing treatment by using macroporous adsorption resin, and preparing sea-buckthorn small molecule peptide powder by using a vacuum freeze-drying technology. According to the method, through ultrasonic-enzyme synergistic interaction, multi-stage purification combination and low-temperature keep-alive processes, the yield, molecular weight uniformity and antioxidant activity of small molecular peptides are remarkably improved, and high-valued utilization of sea-buckthorn processing by-products is achieved.

The present invention provides highly effective and safe means capable of preventing or treating an egg allergy in infants. An oral immunotolerance agent or a food composition for preventing or treating an egg allergy according to the present invention contains a degraded product of heat-denatured egg white with an aspartic protease (e.g. pepsin) or a metal protease (e.g. thermolysin).

The utility model discloses a chromatographic column for purifying pepsin, which comprises a chromatographic column body for separating and purifying pepsin, and a supporting mechanism for keeping the stable state of the chromatographic column body is arranged on the chromatographic column body; the supporting mechanism comprises a limiting assembly for limiting the middle part of the chromatographic column body and a lifting part for supporting which are arranged up and down, and an adjusting part for connection is arranged between the limiting assembly and the lifting part; through cooperative use of the limiting assembly, the lifting part, the adjusting part and the bearing part, the chromatographic column body can be supported, so that the chromatographic column body is kept in a stable state to ensure that operation in pepsin purification related procedures is easy to carry out, the supporting height of the chromatographic column body can be adjusted, and the working efficiency is improved. Extraction devices with different heights can be arranged below the chromatographic column body to carry out extraction operation, so that the application range of the chromatographic column for pepsin purification is widened.

The invention discloses oyster pallium hexapeptide with an effect of promoting osteogenesis as well as a preparation method and application of the oyster pallium hexapeptide. The amino acid sequence of the oyster pallium hexapeptide is Phe-Phe-Glu-Pro-Lys-Phe. Fresh oyster mantle is used as a raw material, pepsin and trypsin are used for hydrolysis and centrifugation to obtain supernate, then ultrafiltration is carried out to obtain filtrate with the molecular weight being 1000 Da or below, finally freeze drying is carried out, the sequence of oyster hydrolytic peptide is obtained through mass spectrum identification, and by means of a molecular docking technology and a database prediction simulation method, the oyster hydrolytic peptide is obtained. Oyster peptide with high osteogenesis promoting capacity is screened from a plurality of peptide sequences. The oyster hexapeptide obtained by the invention has the effects of improving osteoblast proliferation and differentiation capacity and improving dexamethasone-induced zebra fish bone loss, and can be used as a beneficial dietary supplement in related products for delaying bone loss.

The invention belongs to the field of feed protein processing, and particularly relates to a method for improving the bioavailability of animal feed protein through chemical enzymolysis. The method comprises the following steps: mixing a compound protein raw material with water to form a suspension; sodium tripolyphosphate is added for phosphorylation pretreatment, so that protein is subjected to specific modification; after the pH is adjusted to be acidic, pepsase and acid phosphatase are added at the same time for an enzymolysis reaction; and after the reaction is finished, performing enzyme deactivation and drying to obtain the modified feed protein. According to the method, a specific enzyme cutting site is created on a protein molecule through chemical phosphorylation, then directional hydrolysis is achieved through efficient recognition of pepsin on the site, and meanwhile dynamic regulation and control of the hydrolysis process are achieved by means of the dephosphorylation effect of acid phosphatase. The obtained product has the outstanding advantages of high content of small peptides, good digestive absorbability, low bitterness, strong biological activity and the like, and the bioavailability of feed protein is remarkably improved.

The utility model discloses a pepsinogen II detection kit which comprises a heat preservation shell, a refrigeration base movably mounted at the bottom of the heat preservation shell through a second thread, and a temperature conduction mechanism movably inserted into the refrigeration base, and the temperature conducting mechanism is used for rapidly transmitting the refrigeration temperature of the refrigeration base into the heat preservation shell. The pepsinogen II detection kit solves the problems that when an existing pepsinogen II detection kit is used, refrigeration needs to be performed by depending on external refrigeration equipment, so that a detection reagent can be stored for a long time, and the storage temperature of the reagent cannot be guaranteed when the pepsinogen II detection kit is used at high frequency.

The present application relates to the field of eggshell membrane peptide, in order to solve the problem that the proportion of polypeptide with main function of anti-inflammatory effect is low in the eggshell membrane peptide product obtained by hydrolysis of existing eggshell membrane, provide an eggshell membrane peptide and a preparation method thereof, and an application of the eggshell membrane peptide in preparation of anti-inflammatory drugs, comprising: S100, after adding eggshell membrane powder into pure water, adjusting pH, then adding pepsin, enzymolysis for 2-5h, and the enzymolysis temperature is 35-38 DEG C, to obtain a first mixture; S200, the first mixture is subjected to enzyme inactivation process and ultrafiltration process to obtain a first hydrolysate and a first ultrafiltrate; S300, after adjusting the pH value of the first ultrafiltrate to 8-9, adding alkaline protease, enzymolysis for 6-10h, and the enzymolysis temperature is 36-38 DEG C, to obtain a second mixture; S400, the second mixture is subjected to ultrafiltration process to obtain a second ultrafiltrate and a second hydrolysate; S500, after drying the second hydrolysate, a first polypeptide composition is obtained. The present application explores the effect of different hydrolysate components on chondrocyte anti-inflammatory, and screens out eggshell membrane peptides with better effect.

This invention belongs to the field of biomedical technology, specifically relating to a Terminalia chebula active polypeptide, its preparation method, and its application. The preparation method uses Terminalia chebula slices as raw material, which are pulverized, homogenized with water, and then subjected to a series of enzymatic hydrolysis steps: a first hydrolysis with neutral protease, a second hydrolysis with pepsin, and a third hydrolysis with trypsin. Enzyme inactivation is performed after each hydrolysis. Subsequent steps include filtration, removal of alkaline proteins, ultrafiltration, and fine filtration to obtain the Terminalia chebula active polypeptide. The preparation method of this invention, through a three-step progressive enzymatic hydrolysis method, can efficiently prepare highly active Terminalia chebula polypeptides. The obtained Terminalia chebula active polypeptides have small molecular weight, are easily absorbed, and are non-toxic. These active polypeptides exhibit significant anti-tumor and liver fibrosis-improving effects, effectively reshaping the tumor microenvironment, enhancing anti-tumor immune responses, and repairing liver tissue damage. They have broad application prospects in the preparation of anti-tumor and liver fibrosis-improving drugs.

This invention discloses a natural polysaccharide-based multilayer composite encapsulated probiotic and its preparation method, relating to the field of functional food and microbial preparation technology. This invention provides metabolic substrates for the probiotics through a probiotic-prebiotic co-encapsulation core located in the innermost layer, enabling them to maintain physiological activity during encapsulation. The first anionic polysaccharide encapsulation layer uses sodium alginate, which forms a three-dimensional gel network in a calcium-ion-containing curing solution. In the acidic environment of the stomach, the carboxyl groups in the calcium alginate gel undergo carboxylation, and the network shrinks and densifies, effectively blocking the invasion of hydrogen ions and pepsin, thus physically isolating the probiotics from gastric juice. When the encapsulated probiotics enter the small intestine and colon, the chitosan in the second pH-responsive composite encapsulation layer undergoes a conformational change due to amino deprotonation. Simultaneously, phosphate and citrate ions in the outer environment exchange ions with calcium alginate, achieving a triggered release of the probiotics in the colon.

This invention discloses a method for in-situ preparation of calcium chelate peptides that promote intestinal calcium absorption and osteoblast development from fish scales using high-pressure homogenization-assisted enzymatic hydrolysis. The method uses fish scales as raw material, which are pulverized and then pretreated with high-pressure homogenization to break down the dense cross-linked structure of the scales. Pepsin is used as the hydrolytic enzyme to achieve efficient enzymatic hydrolysis of collagen and in-situ chelation with natural calcium, resulting in a one-step preparation of collagen calcium chelate peptides. The product has a calcium retention rate of ≥84.16%, is rich in affinity amino acids, and shows a ≥62.97% increase in calcium transport compared to calcium chloride in a small intestinal epithelial cell absorption and transport model, while being safe and non-cytotoxic. This invention is a green and efficient process that eliminates the need for decalcification steps, achieving a collagen calcium chelate peptide yield of ≥83.4%. It realizes the high-value utilization of aquatic by-products and can be directly applied to various calcium supplements such as food and health products that enhance intestinal calcium absorption and promote bone development, solving problems such as low bioavailability of traditional calcium preparations and severe calcium loss in existing fish scale utilization processes.

The invention discloses a molecular docking screening method and application of hermetia illucens protein antioxidant peptides, and relates to the technical field of biologication.The method comprises the following steps that S1, hermetia illucens protein is prepared through enzymolysis, wherein hermetia illucens defatted protein powder serves as a raw material, enzymolysis is conducted through pepsin under the enzymolysis conditions that the pH is 2.0, the temperature is 40 DEG C, the enzyme adding amount is 3% of the protein mass, the enzymolysis time is 3 h, and enzymolysis liquid is obtained; after the reaction is finished, inactivating, centrifuging, collecting supernatant, and freeze-drying to obtain hermetia illucens protein hydrolysate; according to the molecular docking screening method and application of the hermetia illucens protein antioxidant peptide, the molecular docking technology is specifically applied to efficient screening of the hermetia illucens-sourced antioxidant peptide, KEAP1 is taken as a key target, high-activity candidate peptide is rapidly and accurately positioned from massive enzymolysis peptide fragments, and the screening efficiency of the hermetia illucens-sourced antioxidant peptide is improved. The defects that a traditional blind screening method is large in workload, long in period and high in cost are overcome, and the screening efficiency and the success rate are remarkably improved.

This invention discloses a method and apparatus for modeling the gastric emptying characteristics of compressed dry food. The method includes: obtaining a set of gastrointestinal digestive enzyme concentration ranges; the set of gastrointestinal digestive enzyme concentration ranges includes salivary amylase concentration range, pepsin concentration range, and pancreatic enzyme concentration range; based on the set of gastrointestinal digestive enzyme concentration ranges, performing measurement processing on the compressed dry food to obtain a set of gastric retention rate values; and performing gastric emptying characteristic modeling on the set of gastric retention rate values ​​to obtain a set of gastric emptying models.

The application discloses an amino benzene sulfonamide derivative and application thereof. The structural general formula of the amino benzene sulfonamide derivative is shown in the following formula. The amino benzene sulfonamide derivative has good pepsin inhibitory activity, can effectively block the adhesion and reactivation of pepsin in the pharyngeal mucosa, and has super high selectivity in the internal proteinase family compared with other non-specific protease inhibitors, thereby significantly reducing the off-target risk of normal physiological proteases in the human body. In addition, the compound is particularly suitable for local spray administration in the throat, can maintain a high concentration in the lesion site, and overcomes the defect of traditional oral antacid drugs in the insufficient effect on non-acid reflux, thereby providing a new solution for the treatment of throat reflux diseases.

The invention relates to the technical field of plant extraction, and provides a process for extracting flavone from honeysuckle by using a semi-bionic enzyme extraction method, which comprises the following steps: S1, drying and crushing honeysuckle to obtain honeysuckle powder; s2, dispersing the honeysuckle flower powder in water, adding pepsin, adjusting the pH value to 2.0-4.0, performing enzymolysis for 4-5 hours, and performing centrifugation to obtain first supernate and first residues; s3, immobilized double enzymes are added into the first residues, then the pH is adjusted to 8.0-8.5, enzymolysis is conducted for 5-7 h, and second supernate and second residues are obtained; s4, mixing the first supernate and the second supernate, and performing enzyme deactivation, concentration and vacuum drying to obtain honeysuckle flavone; the immobilized double enzymes comprise the following raw materials: a cellulase solution and a trypsin solution in a volume ratio of 1: (1-5). By means of the technical scheme, the problem that in the prior art, the extraction rate of flavone in honeysuckle is low is solved.

The invention discloses preparation of a beet preparation for promoting disulfide bond exchange in an acid environment and application of the beet preparation to cat hair formation, and relates to the technical field of disulfide bond exchange. A beet molasses extract is prepared through a crystallization technology, and the beet molasses extract is mixed with L-ascorbic acid-6-palmitate and a yeast hydrolysate with high glutathione to prepare the beet preparation. The beet preparation can play roles in promoting disulfide bond exchange and destroying a protein disulfide bond structure in an acid environment. After a cat is fed with the beet preparation, a disulfide bond stable structure can be broken in a cat gastric acid environment, the structural stability of keratin can be reduced, the damage effect of pepsin on cat hair in the cat stomach can be improved, and softening and degradation of the cat hair (cat hair) can be accelerated.

This invention provides a method for rapid extraction of collagen, belonging to the field of regenerative medicine technology. The method includes the following steps: (1) taking animal tissue material as the extraction raw material, crushing it to remove fat and impurities, and obtaining a precipitate; (2) placing the precipitate into a molding mold to obtain a tissue module, freezing and storing the tissue module to obtain a pretreated material; (3) mixing an acidic aqueous solution and pepsin to obtain an extract; placing the pretreated material in the extract in a suspended state, first allowing it to stand and then extracting it in suspension, then stirring, filtering or centrifuging, and retaining the supernatant to obtain a crude extract; (4) adding inorganic salts to the crude extract, allowing it to stand, filtering, collecting the precipitate, washing it, and then transferring the precipitate into a buffer solution to swell it. Filtering the swollen precipitate and dialyzing it to obtain a collagen aqueous solution, followed by post-processing to obtain collagen. The method for extracting collagen provided by this invention can significantly shorten the extraction time.

The invention discloses a bridging antibody, an enzyme-labeled polymer as well as a preparation method and application of the bridging antibody. The bridging antibody is prepared by the following steps: performing enzyme digestion, purification, reduction, sulfydryl protection and coupling on a polyclonal antibody and an anti-DMT-biotin monoclonal antibody through pepsin, and then purifying to obtain a heterodimer product polyclonal antibody-Fab '-Linker-Fab'-anti-DMT-biotin; hRP is subjected to maleimide and then coupled with a sulfhydrylated polymer to obtain a polymer enzyme, and then the polymer enzyme is coupled with a small molecule DMT-biotin to obtain the enzyme-labeled polymer which is specifically combined with the bridging secondary antibody. The obtained bridging antibody and enzyme-labeled polymer are used for preparing a kit for three-stage amplification of immunohistochemical signals, through cooperation of all the components, low-abundance antigens in tissues can be better detected, and the false positive rate is greatly reduced.

The application belongs to the technical field of biomedical raw materials, and particularly relates to a Yanbian yellow cattle bone-derived type I collagen as well as a preparation method and application thereof. The preparation method comprises the following steps: crushing and layering and screening fat-removed Yanbian yellow cattle bones to obtain Yanbian yellow cattle bone particles, gradiently immersing the Yanbian yellow cattle bone particles in acid to obtain mineral-removed Yanbian yellow cattle bone particles, and then removing impure proteins; using pepsin for enzymolysis to obtain an enzymolysis liquid, and then performing salting-out to obtain a crude extract; and further purifying the obtained crude extract to obtain the Yanbian yellow cattle bone-derived type I collagen. The preparation method effectively removes inorganic salts (hydroxyapatite), fat and non-collagen protein impurities in bone tissues, and the content of the finally obtained type I collagen is greater than or equal to 800 mg / g, and the product has high purity.

The invention belongs to the technical field of biology, and particularly relates to a pepsin resistant antibacterial peptide RRI14 and application thereof. The amino acid sequence of the antibacterial peptide RRI14 is Arg-Ile-Arg-Lys-Ile-Ile-Arg-Arg-Ile-Ala-Arg-Arg-Ile-Ile, the molecular weight of the antibacterial peptide RRI14 is 1833.33 Da, the isoelectric point of the antibacterial peptide RRI14 is 12.8, and the antibacterial peptide RRI14 belongs to alkaline polypeptide. The antibacterial peptide RRI14 has broad-spectrum antibacterial activity, can tolerate pepsin hydrolysis and is good in stability. The antibacterial peptide RRI14 disclosed by the invention can be used for preparing a bacteriostatic agent and a feed additive in the field of animal breeding, and has good application potential in the aspect of replacing antibiotics.

The application aims to realize self-adhesive fixation of cartilage repair scaffolds, and provides a collagen scaffold preparation method with controllable removal of terminal peptides, comprising the following steps: S1, cleaning and shearing collagen raw materials to obtain granular collagen materials; S2, dehydrating, centrifuging and dispersing the granular collagen materials to obtain dispersed collagen materials; S3, performing enzymatic treatment on the dispersed collagen materials to obtain a collagen solution; and S4, preparing a collagen scaffold by using the collagen solution, wherein the removal rate of terminal peptides in the collagen solution is controlled at 60%-80% in step S3, which comprises: adding water, glacial acetic acid and pepsin into the dispersed collagen materials according to the mass ratio of dispersed collagen materials:water:glacial acetic acid:pepsin=1:(5-25):(0.3-1.0):(0.05-1.5); keeping at a temperature of 4-8 DEG C for 48 h-120 h, and stirring several times during the keeping period; and performing low-temperature gradient dialysis under a temperature condition of 4-8 DEG C to remove residual enzyme solution, thereby obtaining the collagen solution. The application further provides the collagen scaffold prepared by the method and application thereof.

The application provides king salmon bone collagen peptide for promoting longitudinal growth of bone, a preparation method and application, and relates to the technical field of collagen peptides. The preparation method comprises the following steps: (1) treating king salmon bone raw materials by steam explosion to obtain pretreated king salmon bone; then mixing the pretreated king salmon bone with pepsin and performing first enzymolysis under pressure to obtain a first enzymolysis mixture; mixing the first enzymolysis mixture with collagenase and ficin, performing second enzymolysis under pressure, collecting an enzymolysis liquid, and performing ultrafiltration on the enzymolysis liquid to obtain collagen peptide. In the application, pepsin is used for first enzymolysis under pressure, then collagenase and ficin are used for compounding, the synergistic effect of the three kinds of proteases makes the collagen be fully enzymolyzed, the diversity of the cleavage site is improved, the molecular weight of the polypeptide is reduced, the activity of the polypeptide is improved, the longitudinal growth of the bone is promoted, and the bone density is improved.

The application belongs to the technical field of biological processing, and specifically discloses a sea cucumber flower polypeptide with lipid-lowering efficacy as well as a preparation method and application thereof. The sea cucumber flower polypeptide with lipid-lowering efficacy is composed of short peptides with a molecular weight less than 1000 Da; the short peptides are one or more of GTGATGTF, FTGIVGSL, LAIGETEF, VGITDIESF, VDDEF and YDDVP. The application discloses a sea cucumber flower polypeptide with lipid-lowering efficacy as well as a preparation method and application thereof. The sea cucumber flower polypeptide has a significantly improved inhibition rate on cholesteryl esterase and pancreatic lipase, and has a stronger adsorption capacity on bile acid. The sea cucumber flower polypeptide does not contain the enzyme cutting sites of pepsin and trypsin, can resist secondary digestion and degradation after being taken into the human body, stably maintains structural integrity and biological activity, and solves the problem that traditional lipid-lowering peptides are easily destroyed in activity.