1058 results about "Pepsin" patented technology

A novel composition for treating nervous system disorders. The composition is formed by preparing a mixture comprising an effective amount of vitamin B-6, folic acid, vitamin C, magnesium, vitamin B-3, copper, probiotics, fructo-oligosaccharide (FOS), betaine, pancreatin, papain, pepsin, vitamin B-1, vitamin B-2, vitamin B-12, biotin, pantothenic acid, chromium polynicotinate and a digestive support ingredient selected from the group consisting of dandelion root, juniper, aloe vera, burdock, ginger root, artichoke, and kelp. Other ingredients may include: beta carotene, vitamin E, selenium, zinc, sea vegetation, alfalfa, trace minerals and molybdenum.

The present invention demonstrated the potential use of Lactobacillus johnsonii D115 as a probiotic, as a prophylactic agent or as a surface treatment of materials against human and animal pathogens such as Brachyspira pilosicoli, Brachyspira hyodysenteriae, Shigella sonnei, Vibrio cholera, Vibrio parahaemolyticus, Campylobacter jejuni, Streptococcus pneumoniae, Enterococcus faecalis, Enterococcus faecium, Clostridium perfringens, Yersinia enterocolitica, Escherichia coli, Klebbsiella pneumoniae, Staphylococcus aureus, Salmonella spp., Bacillus cereus, Aspergillus niger and Fusarium chlamydosporum. The proteineous antimicrobial compound was partially characterized and found to be heat tolerant up to 121° C. for 15 min, and acid tolerant up to pH1 for 30 min at 40° C. The compound is also stable to enzymatic digestion, being able to retain more than 60% antimicrobial activity when treated with pepsin and trypsin.

The invention relates to the technical field of biology, and discloses a moringa extract as well as a preparation method of the moringa extract and a moringa feed additive. According to the preparation method, water is added into moringa leaves, the pH value and the temperature are reduced, then, biological enzymes are adopted for enzymolysis, next, heating digestion is adopted to obtain water extraction liquid, ethanol precipitation is carried out after water extraction liquid concentration, obtained precipitates are the moringa extract, the biological enzymes are protease, cellulose and pectinase, and the protease is papain, pepsase, trypsin or serrapeptass. The enzymolysis effects of the specific protease, cellulose and pectinase is utilized for hydrolyzing large blocks of complicated-molecular-structure ingredients of the moringa leaves per se into smaller water-soluble high-bioavailability small molecular active substances, and then, high-yield and high-quality moringa extracts are obtained through a water extraction and ethanol precipitation method.

The invention discloses a latex enhanced turbidimetric immunoassay kit for diagnosing gastric diseases or gastric cancer, a preparation method thereof and application. Components of the kit include diluent, blank solution and a latex reagent of an antibody of pepsinogen I or an antibody of pepsinogen II and can further include a calibrator and quality control serum. The latex reagent contains nanoparticles coupled with the antibody of the pepsinogen I or the antibody of the pepsinogen II, and the particle sizes of the nanoparticles are different. The examination sensitivity cannot meet requirements when latex with the single particle size lower than 100nm is used for examination, the linear range is small when latex with the single particle size of about 200nm is used for examination, and accordingly the examination sensitivity and the linear range cannot meet requirements when latex with the single particle size is used for examination. After composite latex of the kit is used for marking, the examination sensitivity can be improved, the linear examination range can be broadened, and the latex enhanced turbidimetric immunoassay kit has the advantages of fast examination, high sensitivity and specificity, good accuracy and the like in terms of gastric disease or gastric cancer examination.

The invention discloses a new traditional Chinese medicine composition for treating the disease of chronic nephritis and a preparation method thereof. The composition mainly comprises the following raw medicines: tuckahoe, notopterygium root, atractylodes macrocephala koidz, oriental waterplantain rhizome, plantain seed, rhizoma atractylodis, officinal magnolia bark, areca peel, rheum officinale,chrysanthemum, taraxacum officinale root, amur corktree bark, rhizoma cyperi, eucommia bark, zanthoxylum pipertum, talc, Japanese climbing fern spore, glossy privet fruit, epimedium herb, semen alliituberose, angelica, pyrrosia lingua, akebiaquinata, medicated leaven, malt, gizzard pepsin, cape jasmine, immature bitter orange, pericarpium zanthoxyli, Chinese eaglewood, loosestrife, pollen typhaecarbonisatus, cacumen biotae carbonisatus and semen euryales. The composition can be prepared into any one common oral preparation according to the conventional traditional Chinese medicine preparation method. The composition can obviously improve the symptoms such as abdominal fullness and distention, anasarca, fatigue and heaviness, chest stuffiness and poor appetite, aching and limp waist and knees, extreme chilliness, palpitation and severe palpitation and the like, and features confirmed clinical effects, obvious and quick effects. The composition basically combines the medicinal and editable medicines specified in the State Pharmacopoeia, thus having the advantages of low cost and no toxic side effects basically, etc.

The invention discloses a bionic enzymatic product of horns and application thereof, which belong to the field of Chinese medicament. The bionic enzymatic product is characterized in that a bionic enzymatic method is adopted, and comprises the following steps: taking horns; grinding the horns into fine powders; adding water into the fine powders; evenly stirring the mixture; under appropriate condition, performing heat preservation by using pepsin; performing heat preservation by using pancreatin or trypsase; and preparing obtained zymolyte into preparations according to different preparationrequirements. The product of the invention has good effects in the aspects of clearing away heat and toxic material, cooling blood and hemostasis, diminishing inflammation, resisting infection and virus, arresting convulsion, and easing pain.

The preparation methods of globefish skin active collagen and the peptide of the active collagen are that the globefish skin is cut into small pieces to be frozen, dried, crushed and put into sodium chloride solution to be stirred for 12 hours and then for centrifugation to discard supernatant and remove non-collagen proteins, deposition is put in to acetum, pepsin is added under 0 DEG C to 10 DEG C for enzymolysis and centrifugation, the supernatant is put through an ultrafiltration membrane, and the above part is frozen and dried to obtain the collagen. After the active collagen is prepared, the rest deposition is centrifugated until the pH value is between 2.0 to 7.0, papain is added into the deposition for the enzymolysis, the supernatant is centrifugated and respectively put through the ultrafiltration membrane after enzyme-denaturing to obtain the globefish skin collagen peptide of different molecular weight ranges, and the globefish skin collagen peptide are frozen and dried to obtain the light yellow or white powdery globefish skin collagen active peptide which has good antioxidation character and takes effects of eliminating free radical and reducing the senescence degree.

The invention discloses a medicament for treating breast cancer, which comprises the following raw materials: costus root, rhizoma cyperi, radix curcumae, dried orange peel, cortex magnoliae officinalis, dangshen, fried bighead atractylodes rhizome, glossy privet fruit, angelica, milk vetch, Chinese wolfberry, radix scutellariae, coptis, tuber pinellia, morel, solanum lyratum, bupleurum, agrimony, radix rehmanniae, barbed skullcap herb, dandelion, oyster, prepared turtle shell, forsythia, euphorbia helioscopia, citron, corydalis tuber, rhizoma bolbostemmae, American ginseng, white peony root, licorice, glabrous greenbrier rhizome, chuanxiong rhizome, mint, buttercup root, tulip bulb, sarcandra glabra, prunella vulgaris, gizzard pepsin, common burreed rhizome, rhizoma zedoariae, red sage root, fried mastic and fried myrrh. The medicament disclosed by the invention has an obvious effect on treating breast cancer, can be used for treating both principal and secondary aspects of the disease, has the functions of inhibiting the growth and migration of cancer cells, improving the gastrointestinal functions of patients and improving the immunity, and does not have toxic side reactions. The effective rate reaches 92.6%, and the effect taking rate reaches 75%. The medicament disclosed by the invention has the characteristics of exact effect and low price, can be used for quickly curing the breast cancer, and can be easily accepted by patients.

The invention discloses a method for preparing collagen peptide rich in hydroxyproline. The method comprises the following steps: firstly, extracting collagen from anglerfish as a raw material by using an acid liquid, subsequently performing salting-out and dialysis purification, hydrolyzing fishskin collagen by using pepsase and flavourzyme under the condition of ultrasonic wave in different steps, removing the fragrance and the color, performing centrifugal separation, and subsequently freeze-drying so as to obtain the collagen peptide rich in hydroxyproline. By adopting the method, waste of anglerfish skin is effectively utilized, and the collagen peptide rich in hydroxyproline is prepared by hydrolyzing anglerfish skin, so that not only is high nutrition value of the collagen peptide extracted from the anglerfish skin utilized, but also the problems that the waste of the anglerfish skin pollutes the environment and the resource is wasted are solved. The method for preparing the collagen peptide rich in hydroxyproline provided by the invention not only is applicable to a fish skin raw material, but also can be applicable to other raw materials, so that the method has a good application prospect.

The invention discloses an enzyme linked immunosorbent assay kit for combined diagnosis of the gastrosis or evaluation of gastric cancer risks and a preparation method thereof. The kit comprises a micropore plate coated with an antibody against a pepsin antigen I or an antibody against a pepsin antigen II, an enzyme labeled antibody, a color-developing agent, a stop solution and a concentrated cleaning solution, wherein the pepsin antigen I or the pepsin antigen II is a natural protein obtained from extraction of human gastric mucosa tissue. The kit disclosed by the invention adopts a mouse immunized with pepsinogen I and pepsinogen II which are separated from human gastric mucosa to prepare immunogen of a monoclonal antibody, the used standard sample also adopts the pepsin antigen I or the pepsin antigen II separated from the human gastric mucosa, thereby the defects caused by adopting different structures of animal pepsinogen and human pepsinogen are filled. The kit can be used for accurately diagnosing the gastrosis or early gastric cancer and has the advantages of high sensitivity, strong specificity, good accuracy and the like.

The invention relates to a preparation method of a low-molecular-weight colla corii asini hydrolyzate and belongs to the field of medical chemistry. The preparation method includes the following steps: firstly, pure colla corii asini without any auxiliary ingredients is adjusted to a system pH value between 5 and 9 under a constant temperature condition of 30 to 45 DEG C, and then enzyme is added in to have a hydrolysis reaction for 1 to 2 hours; secondly, enzyme is killed through heating; thirdly, colla corii asini hydrolyzate is obtained after cooling or a powder product is obtained through vacuum freeze drying; wherein, the enzyme is one, two or the compound of more than two of pepsin, trypsinase, papain, AS1.398 neutral protease and ficin. The preparation method of a low-molecular-weight colla corii asini hydrolyzate adopts pure colla corii asini as the ingredient and the generated product has high purity and is easier to be absorbed by the human body.

The invention provides a triple-enzyme hydrolysis preparation method of anti-tumor polypeptides of spirulina. The method comprises the following steps: preparing a solution having a concentration of 5 percent from spirulina powder with ultrapure water; repeatedly freezing and thawing, homogenizing and performing ultrasonic treatment, centrifuging to obtain supernate, freezing and drying for later use; adding the prepared protein fluid into pepsin for enzyme hydrolysis, controlling the pH value, adding trypsin for enzyme hydrolysis, adding chymotrypsin for enzyme hydrolysis, controlling the pH value, deactivating enzyme, cooling, and centrifuging to obtain supernate; sequentially filtering with ultrafiltration membranes having molecular weight cut-off of 10KD, 5KD and 3KD respectively to obtain three kinds of spirulina protein enzymatic hydrolysates; carrying out sephadex G15 column chromatography on 0-3K of enzymatic hydrolysate, eluting with water, and collecting four polypeptide ingredients, namely anti-tumor polypeptides of spirulina. The obtained spirulina polypeptide and monopeptide ingredients can be used for establishing a theoretical basis for development and utilization of anti-tumor medicines and health foods.

The invention discloses a method for preparing micromolecular collagen peptide from fish scales by utilizing complex enzyme. The method comprises the steps of deashing and degreasing fish scales used as raw materials; adding complex enzyme mixed by pepsin, papain and trypsin for hydrolyzing, inactivating the enzyme, carrying out coarse filtering, film filtering, vacuum concentrating and freeze drying to obtain the micromolecular collagen peptide. The collagen peptide has the average molecular weight of 500-1,000Da, can be absorbed and utilized by human bodies easily, can be applied to cosmetic, healthcare products, foods, beverages and other industries, and has remarkable economic effect and social effect.

The invention discloses a preparation method of high-purity casein phosphopeptide. The method comprises the following steps: adding water to dissolve casein; carrying out secondary enzymolysis by using compound protease and pepsase prepared from any four of trypsase, neutral protease, alkaline protease, curd protease and nematolyt; carrying out secondary purification treatment through cation exchange resin and anion exchange resin after sterilizing and filtering; washing hydrolysate by water; eluting by hydrochloric acid of which the concentration is 0.5-1%; desalting and drying by a nanofiltration membrane. By adopting the preparation method, the yield (greater than or equal to 17%) and the purity (greater than or equal to 90%) of the casein phosphopeptide can be effectively improved; the prepared casein phosphopeptide is bitter in taste, and good in water-solubility; a water solution containing 20% of casting polypropylene (CPP) is clear and transparent; the casein phosphopeptide is powder of which the color is pure white. The molecular weight of the milk casein phosphopeptide prepared by the method mainly is between 1000 and 5000; absorption of a human body is facilitated; the preparation method disclosed by the invention is a preparation method which is low in cost, simple in process, and applicable to industrial production.

The invention provides a traditional Chinese medicine contrast medium adjuvant used for B ultrasonic, which comprises the following materials in part by weight: 10-20 parts of bighead atractylodes rhizome, 10-20 parts of poria cocos, 10-20 parts of turmeric, 10-20 parts of tangerine peel, 10-20 parts of cortex magnoliae officinalis, 10-20 parts of radix aucklandiae, 10-20 parts of Rhizoma atractylodis, 10-20 parts of cuttlebone, 10-20 parts of calcined oyster shell, 10-20 parts of Chinese yam, 10-20 parts of charred triplet, 10-20 parts of myristica fragrans, 10-20 parts of gizzard pepsin, 10-20 parts of rhizome cyperi, 10-20 parts of codonopsis pilosula, 10-20 parts of combined spicebush root, 10-20 parts of eclipta, 10-20 parts of jasmine, 10-20 parts of baical skullcap root and 10-20 parts of field pennycress. The traditional Chinese medicine contrast medium adjuvant used for B ultrasonic provided by the invention not only can adapt to stomach hyperfunction type, but also can adapt to decreased stomach function type, and also can be applied to the mixed type of various flatulence and mucinosis, check time is short, imaging rate is high, and preparation process is simple and practical. The contrast medium adjuvant has no obvious toxic side effect and has prospect on further development research.

The extraction of active small molecular placentin from lamb embryo and its purpose that belongs to the bioengineering field includes the following steps: removing stomach and intestine from fresh lamb embryo of 40-90 days and cutting up, homogenizing with distilled water, frost thawing repeatedly at -20DEG C, filtrating, heating the filtrate and obtaining Filtrate A and Sediment A; ultra-filtrating Filtrate A and obtaining the transparent primrose yellow Filtrate B and Sediment B; mingling Sediment A and Sediment B, modifying pH to 2.0, adding pepsin, then modifying pH to 7.0, incubating at 100 +-2DEG C and preserving Filtrate C; active small molecular placentin is obtained by mingling Filtrate B and C that can be used in the preparation of skin care product. The placentin prepared in this invention has the molecular weight of 3000-6000 and contains mainly cytokines and small peptides, has high activity, low side-effect such as anaphylactic response, and increased skin absorption ratio.

A beef-flavored essence preparation method, comprising the following steps of: degreasing cleaned ox bones at a high temperature crushing the ox bones by using a crusher after drying treatment for 30 minutes, and sieving by using a 100-mesh sieve to obtain ox bone powder; adding the ox bone powder into a reaction kettle, adding water into the reaction kettle in a material and liquid ratio of 1:14 to prepare an ox bone powder primer solution, adjusting the pH, adding a pepsase for enzymolysis for 3 hours and then adding a compound enzyme in proportion for further enzymolysis, and on the basis, inoculating compound bacteria in a certain proportion to ferment to obtain a bone collagen peptide solution; carrying out Maillard reaction on the bone collagen peptide solution and a reducing sugar to obtain an essence base material; and then mixing the base material with marinade in a certain proportion, blending, concentrating and drying to obtain the beef flavor essence. The beef-flavored essence prepared by the preparation method in the invention has the advantages of being natural in raw material, pure in meat flavor, lasting in fragrance and the like, and is widely applied to foods such as instant noodles, soup bases, fast foods and the like so as to increase or endow the food with a unique meat flavor.

The invention discloses a method of preparing high F-value collagen peptide by hydrolyzing anglerfish fishskins. The method comprises the following steps: by taking anglerfish fishskins as a raw material, extracting collagen by acid liquor; then, fractionally hydrolyzing fishskin collagen by using stomach protease and flavourzyme under an ultrasonic condition; and finally, removing aryl and color, and after vacuum concentration, carrying out spray drying to obtain the high F-value collagen peptide. According to the method disclosed by the invention, leftovers of the anglerfish fishskins are effectively utilized, and the high F-value collagen peptide is prepared by hydrolyzing the anglerfish fishskins, so that not only is high nutritional value of collagen peptide extracted from the anglerfish fishskins utilized, but also the problem that the leftovers of the anglerfish fishskins pollute the environment and lead to waste of resources is further solved. The collagen peptide prepared by the method disclosed by the invention has high F value and small relative molecular weight and is beneficial to be absorbed by the human body. The value of the collagen peptide is higher than that of common collagen peptide.

The invention provides a method for preparing radix astragali homopolysaccharide in the technical field of Chinese medicine. The method comprises the following steps that: radix astragali coarse powder is taken, refluxed and degreased, filtered, refluxed and extracted with water, concentrated, precipitated, centrifuged or filtered to collect precipitate, and the precipitate is washed and dried in vacuum to obtain radix astragali coarse polysaccharide; aqueous solution of the radix astragali coarse polysaccharide is enzymatically hydrolyzed with pepsin, boiled and centrifuged to obtain supernatant fluid; a Sevage agent is added to the supernatant fluid for shaking and extraction so as to collect upper layer aqueous phase solution; diethylaminoethyl cellulose is added to be filtered, concentrated, dialyzed, precipitated, centrifuged or filtered, washed and dried to obtain the refined radix astragali homopolysaccharide; the refined radix astragali homopolysaccharide is separated and purified through column chromatography twice, and eluted with water, the eluant is collected, concentrated, refrigerated and dried to obtain the radix astragali homopolysaccharide. The average molecular weight of the radix astragali polysaccharide obtained in the invention is about 9000 and the radix astragali homopolysaccharide only contains glucose monosaccharide unit after a complete acid hydrolysis.

The invention discloses a sea cucumber egg nutriment and a preparation method thereof. The sea cucumber egg nutriment is white or light yellow hydroscopic powder free of odor and taste and highly soluble to water and ethanol. The preparation method comprises sea cucumber egg and intestine breaking and enzymolysis, metal ion removal through ion exchange, and freeze drying, wherein the enzymolysis employs conjugated enzymes of exogenous enzymes and endogenous enzymes or single exogenous enzymes; single exogenous enzyme enzymolysis refers to the enzymolysis using single pepsinase or using the mixture of trypsinase, dispase and alkaline proteinase. The invention not only makes wastes in sea cucumber processing with traditional methods into an invigorant rich in nutrition but also reduces pollution of the wastes to the environment. The processing method is simple and reasonable and can be industrialized easily.

The invention relates to a method for preparing half-fin anchovy antibacterial peptides, which comprises the following steps of: homogenating fish into fish paste, adding pepsin into the fish paste according to a ratio of 1,000-1,300U/g under the condition that the pH value is 1.5-2.5, raising the temperature to 35-45DEG C, performing enzymolysis for 2 to 3 hours, heating enzymolysis liquid at the temperature of between 95 and 100DEG C for 5 to 15 minutes for killing enzyme, cooling to the temperature of between 3 and 5DEG C, centrifuging at a rate of 6,000 to 8,000r/min for 5 to 15 minutes, filtering, taking intermediate filtrate, and performing frozen storage or freeze-dried storage at -20DEG C at least. The antibacterial peptides is prepared from a pure natural low-value marine product, namely half-fin anchovy; the half-fin anchovy antibacterial peptides are rich in amino acid necessary for human bodies, and have stable physical and chemical properties; the antibacterial peptides prepared from food-derived protein through enzymolysis have high safety, good absorptivity and good antibacterial effect, and can serve as a nutritional and functional additive in foods or is developed into drug resistant bacteria antibiotics. The preparation method is simple, economic and easy to operate, can be carried out in a common laboratory, and is suitable for industrial expanded production.