217 results about "Pepsin" patented technology

The invention discloses a method for promoting dissolution of eggshell membrane protein and preparing chelated calcium through high-pressure crushing. Comprising the following steps: separating and drying eggshells and eggshell membranes of egg shells, and respectively carrying out superfine grinding to obtain eggshell powder and eggshell membrane powder; adding water into the eggshell membrane powder, carrying out high-pressure homogenization, and centrifuging to obtain an eggshell membrane solution; adding eggshell powder into the eggshell membrane solution, adjusting the pH value to 1-3, and adding pepsase for reaction; after the reaction, adjusting the pH value of the eggshell membrane solution to 6.5-7.5, and adding trypsin for reaction; and after the reaction, inactivating the enzyme, centrifuging to take a supernatant, adding absolute ethyl alcohol into the supernatant for alcohol precipitation, and centrifuging to obtain a precipitate, namely the chelated calcium. The technical problem that the eggshell membrane protein is difficult to dissolve out is solved, and a new way is provided for industrial extraction of the eggshell membrane soluble protein.

The invention relates to an oral mucosa repairing medicine and a preparation method thereof, and belongs to the technical field of medicines, the medicine comprises barnacle peptide, salivary lactobacillus, resveratrol, a biphasic adhesion matrix, hydroxyapatite, vitamin B family and other components. The preparation method comprises the following steps: sequentially carrying out enzymolysis on barnacle gooseneck by pepsin and lumbrukinase to obtain barnacle peptide. The double-phase adhesion matrix comprises a water-phase matrix, an oil-phase matrix and lecithin; the water-phase matrix contains a chondroitin sulfate-chitosan quaternary ammonium salt compound, sodium alginate and other components; the oil-phase matrix comprises sesame oil, beeswax, a polylactic acid-glycolic acid copolymer and the like. According to the invention, barnacle peptide and salivary lactobacillus are prepared by adopting a special method and are matched with resveratrol for use, so that multi-target and multi-path promotion of oral mucosa repair can be realized; the chondroitin sulfate-chitosan quaternary ammonium salt compound is prepared through electrostatic and physical embedding, the chondroitin sulfate-chitosan quaternary ammonium salt compound is matched with other components to prepare the biphasic adhesion matrix, the biocompatibility is good, the adhesion is high, and the action time of a sustained-release drug is well prolonged.

The invention discloses camel milk polypeptide with xanthine oxidase inhibitory activity and application thereof.The camel milk polypeptide is prepared by taking fresh camel milk as a raw material, conducting low-temperature centrifugation to remove fat, conducting triple enzymolysis through papain, pepsin and trypsin to obtain enzymatic hydrolysate, enabling the enzymatic hydrolysate to pass through an ultrafiltration membrane (the molecular weight cutoff is 10 kDa) to obtain filtrate, and conducting freeze-drying to obtain the camel milk polypeptide. The compound has xanthine oxidase inhibitory activity. The invention also discloses a small molecule polypeptide with xanthine oxidase inhibitory activity. The filtered solution is screened and combined with polypeptides by adopting a xanthine oxidase affinity ultrafiltration method, the activity of the polypeptides is verified through molecular docking and in-vitro enzyme inhibition, four small-molecule polypeptides with xanthine oxidase inhibitory activity are obtained, the sequence of the small-molecule polypeptides is shown as any one of SEQ ID NO.1-4, and the small-molecule polypeptides can be used for preparing drugs with the effect of inhibiting the uric acid level.

The invention relates to the field of polypeptides, in particular to a red camellia polypeptide as well as a preparation method and application thereof. Red camellia is used as a raw material, high-pressure homogenization wall breaking and pepsase enzymolysis are performed, and separation and purification are performed to obtain the red camellia polypeptide with repairing, moisturizing, oxidation resisting and nourishing functions. The polypeptide prepared by the invention is more efficient, safer and more environment-friendly, and has a wide application prospect in the fields of cosmetics, functional foods and biological medicines.

The invention discloses a bone-strengthening lactoprotein extract as well as a preparation method and application thereof, and belongs to the technical field of deep processing of cow milk. The bone-strengthening milk protein extract contains bone-strengthening active peptide FYPELFR. The preparation method specifically comprises the following steps: (1) pasteurization; (2) separating casein; (3) pepsin hydrolysis; (4) trypsin hydrolysis; (5) purifying and desalting; and (6) concentrating and drying. The bone-strengthening active peptide and the bone-strengthening lactoprotein extract containing the bone-strengthening active peptide can be combined with integrin alpha v beta 1 through hydrophobic interaction and hydrogen bonds, proliferation and differentiation of osteoblasts are promoted by activating a specific signal channel, proliferation of the osteoblasts is promoted, and the bone-strengthening active peptide and the bone-strengthening lactoprotein extract can be used for development of bone-strengthening functional food or drugs and have important significance on deep processing of cow milk.

The invention relates to a kit for detecting pepsinogen I and a preparation method of the kit, and relates to the technical field of biology. The kit comprises an enzyme marker, a luminous marker, a luminous auxiliary agent and a triggering agent, the enzyme marker comprises an anti-PG I monoclonal antibody marked by horse radish peroxidase; the luminous marker comprises an anti-PG I monoclonal antibody marked by 9, 10-dihydroacridine; the light-emitting auxiliary agent comprises H2O2. According to the present invention, the spatial proximity chemiluminescence analysis method is adopted, and the carrier and the washing process are not required, such that the kit cost is reduced, and the detection time is shortened; the method adopts homogeneous reaction, so that the detection result is more real and credible.

The invention belongs to the technical field of active peptides, and particularly relates to an active peptide capable of improving memory, a brain peptide enzymolysis extract and a preparation method and application of the brain peptide enzymolysis extract. The amino acid sequence of the active peptide is shown as SEQ ID NO.1 Ala-Ala-Val-Pro, and the active peptide can improve sleep and improve memory; on the other hand, the invention provides a preparation method of the brain peptide enzymolysis extract. The preparation process comprises the steps of raw material pretreatment and grinding, microwave treatment and centrifugal separation, protein precipitation and enzymolysis, microwave-assisted enzymolysis and purification and nanofiltration membrane filtration. Pepsase is added in the microwave treatment, and the temperature is not more than 50 DEG C in the enzymolysis process, so that the small molecule peptide keeps relatively high activity; furthermore, carbon dioxide gas is introduced in the microwave treatment for aeration, and the aeration pressure and the pH value are optimized, so that the activity and the yield of the brain peptide enzymolysis extract are further improved.

The invention discloses a preparation method of micromolecular active collagen peptide, which comprises the following steps: (1) cutting fresh leather into fragments, pretreating the leather with a sodium bicarbonate solution, rinsing the leather with deionized water, and refrigerating and storing; (2) mixing papain, bromelain, pancreatin, pepsase, alkaline protease, neutral protease and flavourzyme to obtain a compound enzyme preparation; (3) performing high-pressure steam sterilization on the leather at 110-140 kPa and 120-122 DEG C to obtain leather with the hydrolysis degree of 75-85%, then adding distilled water into the leather to obtain mucilage, then adding a compound enzyme preparation into the mucilage, reacting for 5.5-6.5 hours at the pH of 6.8-7.2 and the temperature of 50-55 DEG C, and then performing enzyme inactivation at the temperature of 85-95 DEG C; and (4) after the reaction system is cooled to room temperature, purifying and centrifuging the reaction system, and taking clarified supernate, thereby obtaining the product. Different animal skins are treated, the proportion of peptide fragments with the molecular weight smaller than 1000 Da of the prepared small-molecule active collagen peptide is larger than or equal to 80%, and the hydroxyproline content is larger than 3%.

This invention relates to the field of biocatalysis technology, and more particularly to an aflatoxin oxidase, its preparation method, and its applications. This invention utilizes a self-designed artificial intelligence enzyme design workflow to de novo design a novel aflatoxin oxidase, DnD-AFO. Compared to the traditional wild-type AFO, DnD-AFO exhibits enhanced activity, better thermal stability (its activity is significantly superior to the wild-type after high-temperature granulation), and improved resistance to pepsin and acid. Furthermore, it further enhances the detoxification efficiency against aflatoxin, making it well-suited as a food or feed additive for applications in agriculture and food engineering, among other related industries, demonstrating significant commercial and added value.

This invention relates to the field of chicken gizzard lining processing technology, and particularly to an active polypeptide of chicken gizzard lining, its preparation method, and functional products. The preparation method includes the following steps: mixing chicken gizzard lining powder with water and subjecting it to alkaline heat treatment to form a slurry; sequentially adding Bacillus licheniformis alkaline protease, trypsin, and pepsin to the slurry for three separate hydrolysis processes to obtain a hydrolysate; after enzyme inactivation treatment of the hydrolysate, adding Lactobacillus plantarum for fermentation to obtain a fermentation broth; sterilizing the fermentation broth and then filtering it to obtain a fermentation supernatant; sequentially subjecting the fermentation supernatant to membrane filtration, sterilization, and spray drying to obtain the active polypeptide of chicken gizzard lining. The active polypeptide of chicken gizzard lining prepared by the method of this invention exhibits alcohol dehydrogenase activating activity, AGEs inhibitory activity, albumin denaturation inhibitory activity, lipoxygenase inhibitory activity, pancreatic lipase inhibitory activity, and a beneficial bacteria proliferation promoting effect.

The invention provides a pepsinogen II determination kit and a quantitative determination method, the kit comprises a reagent R1 and a reagent R2, the reagent R1 comprises a luminous donor molecule labeled pepsinogen II first antibody and a reaction buffer solution; and the R2 reagent comprises a light-emitting receptor molecule labeled pepsinogen II second antibody and a reaction buffer solution. A double-antibody sandwich structure marked by a luminescent donor and a luminescent receptor respectively is adopted, binding and luminescence can be completed in a liquid phase, solid phase coating and subsequent multi-step washing are not needed, real homogeneous detection and one-step incubation are achieved, the operation process is simplified, the detection time is shortened, and a full-automatic platform is adapted; and an improved buffer system can inhibit non-specific adsorption and reduce heterotropism interference such as RF / HAMA, so that the detection accuracy and repeatability are improved.

The invention discloses a casein-sourced multifunctional peptide, and belongs to the field of food science and nutriology. The casein is subjected to enzymolysis through pepsin, a method combining activity verification and mass spectrum identification is adopted, the novel multifunctional peptide YPE with a clear sequence is screened and verified, the highest ABTS clearance rate of the casein peptide YPE can reach 77.76%, the highest hydroxyl radical clearance rate of the casein peptide YPE can reach 40.18%, and the casein peptide YPE can be used for preparing the novel multifunctional peptide YPE. The inhibition rate of alpha-glucosidase reaches 30.47%, the inhibition rate of escherichia coli is 10.18%, and the limitation of an existing single-function peptide in application is overcome.

The invention belongs to the technical field of antibodies, and particularly relates to an anti-human pepsinogen I (Pesinogen I, PG I) antibody, an antibody pair and a detection kit. The antibody is a first antibody or a second antibody, amino acid sequences of light chains CDR1-3 of the first antibody are respectively shown as SEQ ID NO.3-5, and amino acid sequences of heavy chains CDR1-3 of the first antibody are respectively shown as SEQ ID NO.8-10; the amino acid sequences of light chains CDR1-3 of the second antibody are respectively as shown in SEQ ID NO.13-15, and the amino acid sequences of heavy chains CDR1-3 of the second antibody are respectively as shown in SEQ ID NO.18-20. The antibody and the antibody pair disclosed by the invention have specific recognition and binding capacity and relatively high affinity and detection sensitivity to human PG I protein, and a reliable antibody tool and a detection means are provided for stably detecting trace-level human PG I protein.

The invention discloses a kit and a method for detecting pepsin based on polypeptide, and belongs to the technical field of proteomics. The amino acid sequence of the polypeptide is as shown in SEQ ID No. 1. The polypeptide is obtained based on enzymolysis of pepsinogen PGA4 and screening, the polypeptide and isotope labeled polypeptide are utilized to establish a standard curve, a biological sample is further subjected to enzymolysis to obtain a peptide fragment sample, and pepsin in the biological sample can be quantified by detecting the content of the polypeptide in the peptide fragment sample. By utilizing the method disclosed by the invention, the pepsin in the biological sample can be rapidly quantified, so that pathological reflux and physiological reflux are diagnosed and distinguished, and the accuracy rate is high.

The invention relates to a chemiluminescence kit for detecting pepsinogen II. The kit comprises an enzyme marker, a luminescent substrate solution and an anti-interference buffer system, the anti-interference buffer system comprises a nonionic surfactant, animal-derived IgG (immunoglobulin G), an antioxidant and catalase; the chemiluminescence kit also comprises a PG II calibration product and a PG II quality control product. The core active component of the enzyme marker is beta-galactosidase. The enzyme marker further comprises an anti-PG II monoclonal antibody marked by beta-galactosidase, bovine serum albumin, trehalose, cane sugar, MgCl2. 6H2O, sodium chloride, Proclin300 and a phosphate buffer solution. According to the kit, a brand-new anti-interference buffer system is introduced on the basis of a spatial proximity chemiluminescence analysis method, so that the resistance to clinically common interferents (such as hemolysis, lipemia, jaundice and heterotropism antibodies) is remarkably improved, and the detection result is more accurate and reliable.

The invention discloses a preparation method of a fructus cannabis protein peptide product with effects of resisting fatigue and improving exercise ability, which comprises the following steps: performing preliminary crushing, ultralow-temperature freezing micro-crushing and low-temperature continuous phase-change extraction degreasing on fructus cannabis, performing step-by-step hydrolysis by using polysaccharase and 2.4 L bacterial protease to extract fructus cannabis protein, performing low-temperature salting-out, and performing freeze-drying to obtain the fructus cannabis protein peptide product with the effects of resisting fatigue and improving exercise ability. The fructus cannabis protein peptide powder is prepared through low-temperature ultrasonic dissolution promotion, high-pressure micro-jet homogenization, compound enzyme C hydrolysis, 1% pepsase, high-temperature enzyme deactivation, chlorophyll removal through counter-current extraction, hydrogen peroxide decoloration, ultralow-temperature instantaneous concentration, low-temperature vacuum belt type drying and low-temperature ultrahigh-pressure sterilization. The fructus cannabis protein peptide prepared by the invention has the following remarkable effects: 1, the exercise ability is remarkably improved, the accumulation of lactic acid and urea nitrogen is reduced, the exhaustion time is prolonged, and the physical and mental fatigue is relieved; 2, the content of hepatic glycogen and muscle glycogen is obviously increased, the energy reserve of the body is increased, the explosive power and the reactivity are improved, and the athletic performance is improved; and 3, the antioxidant level of the body is improved, and fatigue and immune recovery after exercise are promoted.

The invention provides chickpea protein peptide as well as a preparation method and application thereof. The preparation method of the chickpea protein peptide provided by the invention comprises the following steps: sequentially carrying out enzymolysis on chickpea protein isolate by using neutral protease, pepsin and pancreatin; after the enzymolysis is finished, performing ultrafiltration on an enzymolysis product by using an ultrafiltration membrane with the interception amount of 3kDa, and collecting components with the molecular weight of less than 3kDa; and finally, carrying out gel chromatography on the component, and collecting the corresponding component to obtain the chickpea protein peptide. The chickpea protein peptide prepared by the method has inhibitory activity on DPP-IV, alpha-glucosidase and alpha-amylase, can effectively inhibit the activity of Caco-2 cell sucrase, maltase and DPP-IV, and has a certain inhibitory effect on glucose transport; therefore, the chickpea protein peptide provided by the invention has good hypoglycemic activity, and a new thought is provided for development of food-borne functional protein peptides.

The invention discloses a quenching type supramolecular fluorescent probe for pepsin detection as well as a preparation method and application of the quenching type supramolecular fluorescent probe. The quenching type supramolecular fluorescent probe is obtained by virtue of supramolecular interaction of a luminous monomer and a protein molecule; wherein the protein molecule is selected from any one or a combination of more of human serum albumin, bovine serum albumin, casein, hemoglobin and collagen; the luminous monomer comprises an alpha-cyano styrene derivative. The quenching type supramolecular fluorescent probe is easy to synthesize, has good water solubility, can be used as a quenching type fluorescent probe of pepsin, has good selectivity and anti-interference capability, and can well meet the requirements of high sensitivity and accurate detection of pepsin.

The invention provides a PGI monoclonal antibody combination for pepsinogen I detection and application thereof, the PGI monoclonal antibody combination for pepsinogen I detection comprises a first antibody and a second antibody, the first antibody comprises a first light chain variable region and a first heavy chain variable region, and the second antibody comprises a second light chain variable region and a second heavy chain variable region. The second antibody comprises a second light chain variable region and a second heavy chain variable region; wherein the amino acid sequence of the first light chain variable region comprises VL-CDR1-3 as shown in SEQ ID NO: 1-3; the amino acid sequence of the first heavy chain variable region comprises VH-CDR1-3 as shown in SEQ ID NO: 4-6; the amino acid sequence of the second light chain variable region comprises VL-CDR1-3 as shown in SEQ ID NO: 7-9; and the amino acid sequence of the second heavy chain variable region comprises VH-CDR1-3 as shown in SEQ ID NO: 10-12. The monoclonal antibody combination can improve the sensitivity and accuracy of in-vitro detection.

The invention discloses a sea-buckthorn small molecule peptide and a preparation method thereof, and relates to the field of biological medicine and food processing. According to the method, sea-buckthorn pulp residues and sea-buckthorn seed residues are used as composite raw materials and are mixed and stirred to form homogenate, and then cell wall structures are destroyed through ultrasonic wall breaking treatment to release protein components; then cutting off a peptide bond under the action of compound enzymes (plant protease, neutral protease and pepsase) by adopting an ultrasonic-enzyme synergistic hydrolysis process to generate a small molecule peptide fragment; after removing impurities through centrifugal separation, purifying through an ultrafiltration membrane (the molecular weight cut-off is 1-3kDa) to obtain a small molecule peptide solution; and performing debitterizing treatment by using macroporous adsorption resin, and preparing sea-buckthorn small molecule peptide powder by using a vacuum freeze-drying technology. According to the method, through ultrasonic-enzyme synergistic interaction, multi-stage purification combination and low-temperature keep-alive processes, the yield, molecular weight uniformity and antioxidant activity of small molecular peptides are remarkably improved, and high-valued utilization of sea-buckthorn processing by-products is achieved.

The present invention provides highly effective and safe means capable of preventing or treating an egg allergy in infants. An oral immunotolerance agent or a food composition for preventing or treating an egg allergy according to the present invention contains a degraded product of heat-denatured egg white with an aspartic protease (e.g. pepsin) or a metal protease (e.g. thermolysin).

The invention discloses a processing method of pigeon blood tofu. The content of protein peptide and the absorbable utilization degree of protein in a pigeon blood tofu finished product are improved. The method comprises the following steps: (1) anticoagulant treatment: adding an anticoagulant into fresh pigeon blood, and uniformly mixing; (2) extracting the pigeon blood protein: adjusting the pH value of the anticoagulant pigeon blood to the isoelectric point of the pigeon blood protein, centrifuging, and collecting the precipitate to obtain the pigeon blood protein; (3) pre-digestion treatment: carrying out enzymolysis pre-digestion treatment on the pigeon blood protein by using pepsin and trypsin in sequence; (4) making pigeon blood bean curds: adding guar gum, carrageenan, calcium chloride and water into the pre-digested pigeon blood protein, performing reflux treatment by using a colloid mill, and then heating gel to obtain the pigeon blood bean curds; (5) inoculating and fermenting: inoculating debaryomyces hansenii suspension into the pigeon blood tofu, and fermenting; and sterilizing the fermented pigeon blood tofu with steam, and cooling to obtain the pigeon blood tofu product.

The utility model discloses a chromatographic column for purifying pepsin, which comprises a chromatographic column body for separating and purifying pepsin, and a supporting mechanism for keeping the stable state of the chromatographic column body is arranged on the chromatographic column body; the supporting mechanism comprises a limiting assembly for limiting the middle part of the chromatographic column body and a lifting part for supporting which are arranged up and down, and an adjusting part for connection is arranged between the limiting assembly and the lifting part; through cooperative use of the limiting assembly, the lifting part, the adjusting part and the bearing part, the chromatographic column body can be supported, so that the chromatographic column body is kept in a stable state to ensure that operation in pepsin purification related procedures is easy to carry out, the supporting height of the chromatographic column body can be adjusted, and the working efficiency is improved. Extraction devices with different heights can be arranged below the chromatographic column body to carry out extraction operation, so that the application range of the chromatographic column for pepsin purification is widened.

The invention discloses oyster pallium hexapeptide with an effect of promoting osteogenesis as well as a preparation method and application of the oyster pallium hexapeptide. The amino acid sequence of the oyster pallium hexapeptide is Phe-Phe-Glu-Pro-Lys-Phe. Fresh oyster mantle is used as a raw material, pepsin and trypsin are used for hydrolysis and centrifugation to obtain supernate, then ultrafiltration is carried out to obtain filtrate with the molecular weight being 1000 Da or below, finally freeze drying is carried out, the sequence of oyster hydrolytic peptide is obtained through mass spectrum identification, and by means of a molecular docking technology and a database prediction simulation method, the oyster hydrolytic peptide is obtained. Oyster peptide with high osteogenesis promoting capacity is screened from a plurality of peptide sequences. The oyster hexapeptide obtained by the invention has the effects of improving osteoblast proliferation and differentiation capacity and improving dexamethasone-induced zebra fish bone loss, and can be used as a beneficial dietary supplement in related products for delaying bone loss.

The invention belongs to the field of feed protein processing, and particularly relates to a method for improving the bioavailability of animal feed protein through chemical enzymolysis. The method comprises the following steps: mixing a compound protein raw material with water to form a suspension; sodium tripolyphosphate is added for phosphorylation pretreatment, so that protein is subjected to specific modification; after the pH is adjusted to be acidic, pepsase and acid phosphatase are added at the same time for an enzymolysis reaction; and after the reaction is finished, performing enzyme deactivation and drying to obtain the modified feed protein. According to the method, a specific enzyme cutting site is created on a protein molecule through chemical phosphorylation, then directional hydrolysis is achieved through efficient recognition of pepsin on the site, and meanwhile dynamic regulation and control of the hydrolysis process are achieved by means of the dephosphorylation effect of acid phosphatase. The obtained product has the outstanding advantages of high content of small peptides, good digestive absorbability, low bitterness, strong biological activity and the like, and the bioavailability of feed protein is remarkably improved.

The utility model discloses a pepsinogen II detection kit which comprises a heat preservation shell, a refrigeration base movably mounted at the bottom of the heat preservation shell through a second thread, and a temperature conduction mechanism movably inserted into the refrigeration base, and the temperature conducting mechanism is used for rapidly transmitting the refrigeration temperature of the refrigeration base into the heat preservation shell. The pepsinogen II detection kit solves the problems that when an existing pepsinogen II detection kit is used, refrigeration needs to be performed by depending on external refrigeration equipment, so that a detection reagent can be stored for a long time, and the storage temperature of the reagent cannot be guaranteed when the pepsinogen II detection kit is used at high frequency.

The present application relates to the field of eggshell membrane peptide, in order to solve the problem that the proportion of polypeptide with main function of anti-inflammatory effect is low in the eggshell membrane peptide product obtained by hydrolysis of existing eggshell membrane, provide an eggshell membrane peptide and a preparation method thereof, and an application of the eggshell membrane peptide in preparation of anti-inflammatory drugs, comprising: S100, after adding eggshell membrane powder into pure water, adjusting pH, then adding pepsin, enzymolysis for 2-5h, and the enzymolysis temperature is 35-38 DEG C, to obtain a first mixture; S200, the first mixture is subjected to enzyme inactivation process and ultrafiltration process to obtain a first hydrolysate and a first ultrafiltrate; S300, after adjusting the pH value of the first ultrafiltrate to 8-9, adding alkaline protease, enzymolysis for 6-10h, and the enzymolysis temperature is 36-38 DEG C, to obtain a second mixture; S400, the second mixture is subjected to ultrafiltration process to obtain a second ultrafiltrate and a second hydrolysate; S500, after drying the second hydrolysate, a first polypeptide composition is obtained. The present application explores the effect of different hydrolysate components on chondrocyte anti-inflammatory, and screens out eggshell membrane peptides with better effect.

This invention belongs to the field of biomedical technology, specifically relating to a Terminalia chebula active polypeptide, its preparation method, and its application. The preparation method uses Terminalia chebula slices as raw material, which are pulverized, homogenized with water, and then subjected to a series of enzymatic hydrolysis steps: a first hydrolysis with neutral protease, a second hydrolysis with pepsin, and a third hydrolysis with trypsin. Enzyme inactivation is performed after each hydrolysis. Subsequent steps include filtration, removal of alkaline proteins, ultrafiltration, and fine filtration to obtain the Terminalia chebula active polypeptide. The preparation method of this invention, through a three-step progressive enzymatic hydrolysis method, can efficiently prepare highly active Terminalia chebula polypeptides. The obtained Terminalia chebula active polypeptides have small molecular weight, are easily absorbed, and are non-toxic. These active polypeptides exhibit significant anti-tumor and liver fibrosis-improving effects, effectively reshaping the tumor microenvironment, enhancing anti-tumor immune responses, and repairing liver tissue damage. They have broad application prospects in the preparation of anti-tumor and liver fibrosis-improving drugs.

This invention discloses a natural polysaccharide-based multilayer composite encapsulated probiotic and its preparation method, relating to the field of functional food and microbial preparation technology. This invention provides metabolic substrates for the probiotics through a probiotic-prebiotic co-encapsulation core located in the innermost layer, enabling them to maintain physiological activity during encapsulation. The first anionic polysaccharide encapsulation layer uses sodium alginate, which forms a three-dimensional gel network in a calcium-ion-containing curing solution. In the acidic environment of the stomach, the carboxyl groups in the calcium alginate gel undergo carboxylation, and the network shrinks and densifies, effectively blocking the invasion of hydrogen ions and pepsin, thus physically isolating the probiotics from gastric juice. When the encapsulated probiotics enter the small intestine and colon, the chitosan in the second pH-responsive composite encapsulation layer undergoes a conformational change due to amino deprotonation. Simultaneously, phosphate and citrate ions in the outer environment exchange ions with calcium alginate, achieving a triggered release of the probiotics in the colon.