243 results about "Dexamethasone" patented technology

The invention provides an adipose-derived stem cell composition for wound repair as well as a preparation method and application thereof, and belongs to the technical field of stem cells. The method comprises the following steps: adding fetal calf serum, dexamethasone, penicillin, streptomycin, collagen, magnetic particles and a P substance into a DMEM culture medium to prepare a culture medium, performing low-frequency magnetic field culture and separation on adipose-derived stem cells to obtain a stem cell solution, performing embedding to obtain stem cell microspheres, freeze-drying supernate to obtain a paracrine culture substance, and performing freeze-drying on the paracrine culture substance to obtain the adipose-derived stem cells. The adipose-derived stem cell composition for wound repair is prepared by mixing the adipose-derived stem cell with stem cell microspheres, hydroxypropyl methyl cellulose, carboxymethyl chitosan, hyaluronic acid, glycerol and stem cell microspheres, has better effects of implanting into damaged tissues, releasing nutrient substances, promoting neovascularization, regulating oxidative stress and inducing anti-inflammatory response, is beneficial to wound repair, and has a good application prospect. The gel can be converted into a gel phase at the skin epidermis temperature of 35-37 DEG C, components are stabilized, and sustained and controlled release is realized.

The present disclosure relates to a method of treating diabetic macular edema in a human subject in need thereof, said method comprising(i) an induction phase of topically administering to an affected eye of said subject, an eye drop formulation comprising 1.5% (w / v) dexamethasone, at a dosing of 5 to 7 drops a day for 3, 4, 5, 6 weeks, followed by,(ii) a maintenance phase of topically administering to an affected eye of said subject, said eye drop formulation comprising 1.5% (w / v) dexamethasone at a daily dosing less frequent than the induction phase for example 1-3 drops.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to an SCRE10 gene for improving rice disease resistance and application of the SCRE10 gene, the base sequence of the SCRE10 gene is shown as SEQ ID NO.1, and the amino acid sequence of the SCRE10 gene is shown as SEQ ID NO.2. Through construction of a dexamethasone induced expression SCRE10 transgenic rice plant, it is found that the transgenic rice can significantly induce PR gene expression and active oxygen outbreak, and the resistance of rice to false smut and bacterial leaf blight can be improved. The invention proves that heterologous inducible expression of the Ustilaginoidea virens SCRE10 gene has the function of positively regulating the disease resistance of the rice, and the SCRE10 gene can be used for improving the disease resistance of the rice, which is of great significance to the creation of disease-resistant germplasm of the rice.

The invention belongs to the technical field of biological medicines, and particularly relates to a pyruvate preparation for treating asthma and a preparation method of the pyruvate preparation. The pyruvate preparation is prepared from the following components in parts by weight: 10 to 12 parts of pyruvate, 10 to 12 parts of sodium chloride, 0.5 to 1 part of citric acid and 500 parts of water for injection. The pH value of the pyruvate preparation is 6 to 7. The pyruvate preparation is used for daily management of asthma patients, the purpose of inhibiting asthma is achieved by controlling increase of the number of inflammatory cells such as eosinophilic granulocytes and reducing inflammatory reaction, the effect of the pyruvate preparation is close to that of similar hormone medicine dexamethasone, and the effect of the pyruvate preparation is improved while the treatment purpose is achieved. The dependence and side effects of patients on hormone drugs are reduced.

The invention discloses a serum-free adipogenic differentiation method for porcine adipose precursor cells, and relates to the technical field of mammalian animal cell differentiation.In the adipogenic differentiation process, an induced differentiation culture medium and a maintained differentiation culture medium are sequentially used, and the induced differentiation culture medium is composed of a basic culture medium and cell culture auxiliary factors; the cell culture auxiliary factor is composed of 66nmol / L insulin, 33 [mu] mol / L biotin, 17 [mu] mol / L pantothenic acid, 0.1 [mu] mol / L dexamethasone, 0.25 mmol / L IBMX, 1 [mu] mol / L rosiglitazone, 150 [mu] mol / L sodium oleate and 10 [mu] g / mL transferrin; the maintenance differential medium removes IBMX and rosiglitazone on the basis of inducing the differential medium. The serum-free adipogenic differentiation method for the porcine fat precursor cells, provided by the invention, is good in differentiation effect and high in repeatability.

The invention provides a stem cell extract composition for promoting hair growth and a preparation method thereof, and belongs to the technical field of cosmetics. Fetal calf serum, dexamethasone, penicillin, streptomycin, interleukin-2 and magnetic composite particles are added into a DMEM culture medium to prepare the culture medium, the culture medium is used for in-vitro culture of stem cells, during culture, a stem cell solution is prepared by combining a low-frequency magnetic field with low-energy laser culture, exosomes are extracted from an extracting solution, the exosomes and the stem cells are mixed and embedded to prepare embedded microspheres, and the embedded microspheres are used for in-vitro culture of the stem cells. The stem cell extract composition for promoting hair growth is prepared by uniformly mixing the stem cell extract with extracts extracted from fresh ginger, ginseng and polygonum multiflorum, the storage stability of stem cells and exosomes is obviously improved, hair growth is promoted, hair follicle development is promoted, mouse hair is induced and maintained in a growth period, and the stem cell extract composition is free of hormone addition, safe and non-irritant, and has a good application prospect. Wide application prospects are realized.

The invention provides application of lactobacillus plantarum Lp-G18 in preparation of a medicine for preventing and / or treating skeletal muscle atrophy, and belongs to the technical field of biological medicines. The invention further provides a preparation method of the lactobacillus plantarum Lp-G18 for preventing and / or treating skeletal muscle atrophy. According to the invention, a dexamethasone (DEX)-induced C2C12 amyotrophy cell model is taken as an experimental subject to evaluate the efficacy of the phytobacterium plantarum strain Lp-G18 in the aspect of skeletal amyotrophy treatment, and the phytobacterium plantarum strain Lp-G18 has a remarkable improvement effect on DEX-induced myotube diameter reduction, fusion index reduction, protein content reduction and myotube length shortening. Therefore, the lactobacillus plantarum strain Lp-G18 can be applied to prevention and / or treatment of skeletal muscle atrophy and can be used as an active component to develop and prepare related medicines.

The invention relates to the technical field of stem cell induced differentiation, and discloses an umbilical cord mesenchymal stem cell adipogenic induced differentiation culture medium and a culture method. The culture medium comprises the following substances: a DMEM (Dulbecco's Modified Eagle Medium) / F12 basal culture medium, fetal calf serum, insulin, penicillin, streptomycin, dexamethasone, 3-isobutyl-1-methylxanthine, indometacin, BMP-2 and FGF-1 (Fibroblast Growth Factor 1). The culture method comprises the following steps: (1) carrying out primary isolation culture on umbilical cord mesenchymal stem cells; (2) carrying out passage on the umbilical cord mesenchymal stem cells; and (3) inducing adipogenesis of the umbilical cord mesenchymal stem cells by using the adipogenesis induced differentiation culture medium. BMP-2 and FGF-1 are added on the basis of a traditional adipogenic induction culture medium to induce and promote the umbilical cord mesenchymal stem cells to be more efficiently differentiated into adipocytes under the combined action, so that the adipogenic differentiation time is shortened, and the adipogenic differentiation efficiency is improved; the umbilical cord mesenchymal stem cell adipogenic culture medium is stable in formula, simple and convenient in culture method and suitable for wide popularization.

The invention belongs to the technical field of cell biology, and particularly discloses a culture medium and a culture method of fish primary liver cells. The culture medium of the fish primary liver cells comprises the following components: 90% of an M199 culture medium, 10% of fetal calf serum, 100 U / mL of penicillin, 0.1 mg / mL of streptomycin, 5 ng / mL of EGF, 10 [mu] U / ml of insulin and 10 mM of dexamethasone. Based on the culture medium, an efficient and stable fish liver cell culture method is developed by combining a standardized anesthesia and disinfection process, a precise dissection and liver sampling method, an optimized tissue digestion and cell separation scheme, an efficient cell purification and adherent culture system, a stable passage technology and the like; a standardized hepatocyte line establishment operation process is defined, and the problems that species binding exists in an existing fish liver cell culture method, and a standardized culture process suitable for multiple fish fingerlings is difficult to form are solved.

The invention discloses an umbilical cord mesenchymal stem cell serum-free culture medium which is composed of a basic culture medium and an additive, and the basic culture medium is DMEM / F12. The additive is prepared from albumin, transferrin, insulin, a fibroblast growth factor (FGF), an epidermal growth factor (EGF), dexamethasone, glutamine, ascorbic acid, recombinant human III type collagen and recombinant human XVII type collagen. According to the UCMSCs obtained by culturing the umbilical cord mesenchymal stem cells by using the serum-free culture medium, the cellular morphology and surface marker expression are maintained; the umbilical cord mesenchymal stem cell serum-free culture medium has the advantages that the cell proliferation capacity, the monoclonal performance capacity and the three-line differentiation capacity are enhanced, the umbilical cord mesenchymal stem cell serum-free culture medium only contains 12 raw materials, the compounding process is simple, the cost is low, the raw materials of the umbilical cord mesenchymal stem cell serum-free culture medium are clear in quality, and the umbilical cord mesenchymal stem cell serum-free culture medium can be used for culturing clinical-grade UCMSCs.

The present disclosure relates to a method for stabilizing the pH of an aqueous composition comprising a drug which is prone to oxidation, said method comprising the addition of an additive to prevent oxidation of the drug which is prone to oxidation. In particular, the present disclosure relates to a method for stabilizing the pH of an aqueous composition comprising a corticosteroid, said method comprising the addition of an additive to prevent oxidation of the corticosteroid. The present disclosure also relates to a composition comprising a corticosteroid and an additive to prevent oxidation of the corticosteroid.

The present invention relates to the field of cell culture, and particularly to a serum-free induction adipogenic differentiation medium for fish stem cells and its application. It is composed of a basal medium and a combination of cell culture cofactors; the basal medium is one of DMEM and DMEM / F12 medium; the combination of cell culture cofactors consists of oleic acid, soybean lecithin, vitamin C phosphate, β-cyclodextrin, transferrin, saikosaponin A, bakuchiol, dehydroabietic acid, and phenyl lactic acid. By adding the combination of cell culture cofactors, the present invention replaces the serum in the traditional adipogenic induction differentiation medium for preadipocytes and the core component dexamethasone IBMX in the traditional adipogenic differentiation "cocktail", and obtains a medium suitable for serum-free induction adipogenic differentiation of fish stem cells, which not only solves the bottleneck problems of the prior art, but also provides reliable technical support for the large-scale production of cell-cultured fish meat.

The invention relates to the field of cell culture, in particular to a serum-free induced adipogenic differentiation culture medium for fish stem cells and application of the serum-free induced adipogenic differentiation culture medium. The culture medium consists of a basic culture medium and a cell culture auxiliary factor combination, the basic culture medium is one of a DMEM (Dulbecco's Modified Eagle Medium) and a DMEM / F12 culture medium; the cell culture auxiliary factor combination is prepared from oleic acid, soya bean lecithin, vitamin C phosphate, beta cyclodextrin, transferrin, saikoside A, psoralen flavanone methyl ether, dehydroabietic acid and phenyllactic acid. The culture medium suitable for serum-free induced adipogenic differentiation of fish stem cells is obtained by adding a cell culture auxiliary factor combination to replace serum in a traditional adipose precursor cell adipogenic induced differentiation culture medium and dexamethasone IBMX serving as a core component of traditional adipogenic differentiation cocktail, the bottleneck problem in the prior art is solved, and the culture medium is suitable for serum-free induced adipogenic differentiation of fish stem cells. And reliable technical support is also provided for large-scale production of cell culture fish meat.

The invention belongs to the technical field of cell culture, and particularly relates to a serum-free mesenchymal stem cell culture medium and a preparation method thereof. The serum-free mesenchymal stem cell culture medium provided by the invention is prepared by taking a DMEM (Dulbecco Modified Eagle Medium) / F12 basal culture medium as a basis and adding supplementary factors, namely yeast hydrolysate, insulin, albumin, dexamethasone, taurine, salidroside, soy isoflavone, baicalein, human platelet lysate, momordica saponin and hyperoside. According to the serum-free mesenchymal stem cell culture medium provided by the invention, the normal form of cells can be maintained without supplementing serum, rapid proliferation is realized, and the biological characteristics of the mesenchymal stem cells are maintained.

The present disclosure relates to a method for stabilizing the pH of an aqueous composition comprising a drug which is prone to oxidation, said method comprising the addition of an additive to prevent oxidation of the drug which is prone to oxidation. In particular, the present disclosure relates to a method for stabilizing the pH of an aqueous composition comprising a corticosteroid, said method comprising the addition of an additive to prevent oxidation of the corticosteroid. The present disclosure also relates to a composition comprising a corticosteroid and an additive to prevent oxidation of the corticosteroid.

The invention provides a nano-micelle responding to an inflammation microenvironment as well as a preparation method and application of the nano-micelle. According to the nano-micelle, polyethylene glycol (PEG)-poly L-glutamic acid (PLG) is grafted with 4-hydroxymethyl phenylboronic acid (PBA), and the drug loading capacity of a hydrophobic anti-inflammatory drug is improved based on a hydrophobic effect and a borate bond formed by the PBA and a 1, 3-diol or 1, 2-diol structure of dexamethasone, curcumin and luteolin. The nano-micelle (PPDex micelle) has reversible splitting and rapid release behaviors under an inflammatory condition (the ROS level is increased or the pH value is reduced). The nano-micelle has a proper particle size, can effectively relieve inflammatory cell infiltration and pulmonary edema in the lung of an acute lung injury (ALI) mouse after pulmonary administration, reduces pro-inflammatory cytokines in the lung, inhibits oxidative stress, promotes polarization of macrophages from pro-inflammatory M1 phenotype to anti-inflammatory M2 phenotype, and finally inhibits the progress of ALI. The invention provides a novel effective anti-inflammatory strategy.

The invention discloses a culture medium for adipose-derived stem cells, and particularly relates to the technical field of stem cell culture. The culture medium comprises a basic culture medium and nerve-vascular niche components, wherein the basic culture medium comprises DMEM / F12, 10% of human-derived platelet lysate and 1% of antibiotic solution; the nerve-vascular niche comprises the following components: a Slit3 recombinant protein, a nerve growth factor-beta, a glial cell-derived neurotrophic factor, a lysyl oxidase inhibitor, dexamethasone, 3-isobutyl-1-methylxanthine and insulin. The traditional fetal calf serum is replaced by the human-derived platelet lysate, so that the risks of immunological rejection and pathogen pollution are avoided; through the synergistic effect of nerve-vessel niche factors, blood vessel maturation and innervation are promoted, programmed mechanical stretching culture is combined, the adipogenic differentiation efficiency of adipose-derived stem cells is remarkably improved, and engineered adipose tissue which is huge in lipid droplet, compact in structure and provided with a nerve-vessel network is formed.

The invention belongs to the field of biological medicine, and relates to a rosmarinic acid and scutellarin compatible composition and application thereof. The invention specifically discloses a rosmarinic acid and scutellarin composition as shown in formulas (I) and (II), and the rosmarinic acid and scutellarin composition is applied to treatment and / or prevention of acute lung injury. The screened rosmarinic acid and scutellarin composition is simple in preparation method, has a potential synergistic treatment effect on acute lung injury, has a remarkable treatment effect on lung functions, alveolar lavage fluid inflammatory factors and serum inflammatory indexes of LPS-induced mouse acute lung injury, and has a good application prospect. And compared with the toxic and side effects of dexamethasone on reducing body weight, the dexamethasone shows good safety, and meanwhile, the dexamethasone shows a remarkable inhibition effect on secretion of primary peritoneal macrophages NO, TNF-alpha and IL-6 in vitro. In conclusion, the rosmarinic acid and scutellarin composition can be in the form of a single composition or a medicinal composition, and has potential application value in treatment of acute lung injury and immune-related diseases. # imgabs0 #

The embodiment of the present invention discloses an induction culture medium, method and application for improving the viability and factor secretion ability of hair follicle-derived mesenchymal stem cells in a hypoxic environment. The induction culture medium includes a basal culture medium and an induction drug, and the induction drug includes transforming growth factor β, hepatocyte growth factor, cyclic adenosine monophosphate, triptolide, sodium hydrosulfide, lipopolysaccharide, hyaluronic acid, dexamethasone, L-glutamine and HEPES buffer. The induction culture medium provided by the present invention can promote the metabolism and energy production of hair follicle-derived mesenchymal stem cells, enhance the antioxidant capacity of cells, improve the survival rate and factor secretion ability of cells in a hypoxic environment, and solve the problems of low cell viability, slow growth, low cytokine secretion, etc. after transplanting cells into damaged tissue areas in the prior art.

The invention discloses a hexapeptide LR6 with anti-inflammatory activity and a preparation method and application thereof, and belongs to the technical field of small molecule peptides. The amino acid sequence of the hexapeptide LR6 is LDAVDR, and the hexapeptide LR6 can be prepared through a solid-phase synthesis method and an enzymolysis method. The hexapeptide LR6 is identified from spirulina platensis proteolysis liquid and has potential interaction with Keap1, an LPS-induced RAW264.7 cell test shows that TNF-alpha, IL-1beta and IL-10 can be remarkably inhibited by treatment of low-dose (200mM) and high-dose (400mM) hexapeptide LR6, the inhibition effect of the high-dose hexapeptide LR6 is superior to that of positive control dexamethasone, and the inhibition effect of the high-dose hexapeptide LR6 is superior to that of positive control dexamethasone. Compared with dexamethasone, the hexapeptide LR6 has a better anti-inflammatory effect and can be used for preparing anti-inflammatory drugs.

The present invention is part of the field of pharmacology and medicine and relates to the use of coumarin derivative compounds for the treatment of asthma and / or chronic obstructive pulmonary disease (COPD). The present inventors identified that inhaled braylin has high therapeutic efficacy in the treatment of asthma and COPD, with efficacy comparable to systemic dexamethasone, and presenting itself as an unprecedented alternative to conventional treatments.

It was confirmed that probiotic metabolites of the present invention protect muscle cells and promote differentiation of muscle fibers. In addition, it was confirmed in an animal model of muscle atrophy or sarcopenia induced by dexamethasone that probiotic metabolites increased muscle mass and muscle function, inhibited expression of factors associated with muscle degradation, and increased expression of factors associated with muscle synthesis and regeneration. It was confirmed that probiotic metabolites contribute to alleviation of muscle atrophy by regulating sugar, amino acid, and fatty acid metabolism altered by dexamethasone.

The invention discloses a duck skeletal muscle satellite cell serum-free myoblast induced differentiation culture medium and an induction method and application, and relates to the technical field of animal cell differentiation. The cell culture auxiliary factor is composed of 0.1 [mu] mol / L of insulin, 1 [mu] mol / L of dexamethasone, 1 [mu] g / mL of linoleic acid, 10 [mu] g / mL of transferrin and a 1 * GlutaMAX supplement. The duck skeletal muscle satellite cell serum-free myoblast induced differentiation culture medium provided by the invention does not contain any serum component, has definite chemical components and good differentiation effect, and is convenient for promoting the industrialization process of cell culture meat.

Pharmaceutical compositions, methods for treating various issues of the eyes, and methods of preparing such compositions are described. These pharmaceutical compositions may be for treating glaucoma, in preparation of eye surgery, during eye surgery, various post-op care (e.g., after cataract surgery, laser eye surgery, and the like), for treating dry eyes, and / or for promoting eyelash growth. These pharmaceutical compositions may comprise such active ingredients (APIs) as: timolol, latanoprost, brimonidine tartrate, dorzolamide, moxifloxacin HCl, dexamethasone PO4, phenylephrine HCl, lidocaine HCl, ketorolac tromethamine, bromfenac, prednisolone PO4, gatifloxacin, amniotic cytokine extract (ACE), prostaglandin E2 (PGE2), and combinations thereof.

A lipid nanoparticle composition for use in the delivery of a self-amplifying RNA (saRNA) construct in one or more target cells is disclosed. The lipid nanoparticle composition comprises a lipid mixture comprising at least one (ionizable) cationic lipid, at least one helper lipid, a sterol, a corticosteroid such as Dexamethasone, and a least one lipid-polyethylene glycol conjugate. The saRNA construct comprises a first open reading frame which encodes one or more non-structural proteins, and a second open reading frame operatively linked to the first open reading frame. The second open reading frame comprises a coding region which encodes one or more target proteins. In some embodiments, the target proteins comprise one or more therapeutic proteins. In some embodiments, the target proteins comprise one or more one or more Cas proteins and / or variants thereof for use in CRISPR-based gene editing.

The invention belongs to the technical field of natural medicines, and particularly relates to a method for increasing the content of catharanthine in hairy roots of catharanthus roseus, which is characterized by comprising the following steps: extracting RNA (Ribonucleic Acid) of the catharanthus roseus, carrying out reverse transcription to obtain cDNA (Complementary Deoxyribonucleic Acid), carrying out PCR (Polymerase Chain Reaction) amplification by taking the cDNA as a template to respectively obtain GS (Glutathione S) and CS (Citrus S), with the nucleotide sequence of the GS as shown in SEQ ID NO.9 and the nucleotide sequence of the CS as shown in SEQ ID NO.10; gS and CS are jointly cloned into an overexpression vector, and a double-gene expression vector is obtained; infecting the catharanthus roseus by using the double-gene expression vector; the infected catharanthus roseus is sequentially subjected to screening culture and subculture, an inducer is added in the subculture process, and the inducer is dexamethasone. Through the method provided by the invention, the content of catharanthine in the catharanthus roseus can be increased by 67% or above. The method for increasing the content of the vinblastine in the hairy roots of the catharanthus roseus is established, and a foundation is laid for large-scale production of the direct precursor vinblastine of an anti-cancer drug through the hairy roots.

The invention belongs to the technical field of biological medicine research, and particularly relates to a cell in-vitro calcification inducer, an induction culture medium and application thereof, an aortic valve calcification in-vitro induction model and a modeling method thereof. The inducer provided by the invention is prepared from the following components in parts by weight: 2160.4 parts of beta-sodium glycerophosphate, 0.039 parts of dexamethasone, 50 parts of vitamin C and 34 parts of vitamin D. The invention further provides a culture medium formed by using the inducer and a method for modeling an aortic valve calcification in-vitro induction model by using the inducer. According to the method, osteogenic differentiation of the aortic valve interstitial cells can be stably induced within a short time, an efficient and repeatable standardized induction scheme is provided for in-vitro aortic valve calcification models and drug screening, and the method has a very good application prospect.

The invention relates to an application of Quercetin (QUE) or a derivative thereof in a medicine for resisting vocal cord fibrosis and a preparation of the Quercetin or the derivative thereof. The quercetin can significantly inhibit abnormal deposition of extracellular matrix (ECM) after vocal cord injury, improve reduction of hyaluronic acid (HA) and elastic fibers (EF), and reduce overexpression of type I collagen (COL1A1) and fibronectin (FN1). Besides, by regulating PI3K / AKT and MAPK signal channels, the quercetin inhibits proliferation and migration of fibroblasts and promotes apoptosis of the fibroblasts, so that vocal cord fibrosis is effectively relieved. The invention also provides an application of a preparation of the quercetin and the derivative thereof combined or not combined with other anti-fibrosis drugs (such as 5-fluorouracil and dexamethasone) in treatment of vocal cord fibrosis, wherein the dosage forms of the preparation comprise an oral preparation, an injection, a spray or a local gel preparation.

The present invention provides methods for treating multiple myeloma (e.g., refractory multiple myeloma or recurrent and refractory multiple myeloma) in an individual who has received at least two prior therapies for multiple myeloma. The methods comprise administering to the individual an anti-CD38 antibody, pomalidomide, and dexamethasone. Methods of ameliorating renal damage in an individual having multiple myeloma are also provided.

The present invention relates to a composition for strengthening muscle strength or preventing, ameliorating or treating muscle loss, comprising a Lilium lancifolium extract as an active ingredient. Specifically, when C2C12 cells were pre-treated with a Lilium lancifolium extract, and then treated with H2O2, dexamethasone, or TNF-α, muscle damage was alleviated and muscle loss was inhibited, and the muscle mass and grip strength decreased by dexamethasone administration in a muscle atrophy mouse model were increased upon administration of the Lilium lancifolium extract. Therefore, the composition can be effectively used as a health functional food, medicine or feed additive for strengthening muscle strength or preventing, ameliorating or treating muscle loss.