The invention discloses a miRNA composition for detecting breast cancer brain metastases and a kit with same. The miRNA composition comprises miR-10b and one or more of miR-200a, miR-122, miR-21 and miR-181c. The kit comprises forward and reverse primers of the miRNA composition, forward and reverse primers of an external reference U6 RNA, a cerebrospinal fluid RNA extraction system, a reverse transcription reagent, a PCR amplification reagent and a cDNA mixed positive reference product. According to the miRNA composition, a group of miRNAs are adopted as novel breast cancer brain metastases markers, low specificity and low sensitivity caused by tumor heterogeneity are hard to overcome by using a single marker can be alleviated, prognosis effectiveness evaluation of a patient suffering from breast cancer can be effectively improved, and thus clinical treatment can be instructed. The miRNA composition is used for preparing a kit or a biological chip for prognosis inspection on breast cancer by using a fluorescent quantitative/digital PCR method, low specificity and low sensitivity caused by tumor heterogeneity can be avoided, the prognosis effectiveness evaluation of the patient suffering from breast cancer can be effectively improved, and thus clinical treatment can be instructed.