906 results about "Combined treatment" patented technology

The invention discloses a near-infrared emission aggregation-induced emission organic photo-thermal molecule and a preparation method and application thereof, and belongs to the technical field of biomedicine, and the structure is shown as a formula I: # imgabs0 # (I), wherein R1, R2, R3, R4, R5 and R6 are independently selected from groups containing alkyl chains. The invention also provides a preparation method of the organic photo-thermal molecule and application of the organic photo-thermal molecule in preparation of multifunctional protein nanoparticles with near-infrared aggregation-induced emission. The near-infrared emission aggregation-induced emission organic photo-thermal molecule provided by the invention shows excellent near-infrared imaging effect and photo-thermal capability under laser irradiation, and the obtained near-infrared aggregation-induced emission multifunctional protein nano particle has excellent photo-thermal effect and imaging resolution, has excellent targeting to brain tumor, and has good application prospects in blood brain barrier crossing, brain tumor targeting and brain tumor targeting. And tumor killing, photothermal and ferroptosis combined treatment has practical medical significance, and is expected to be widely applied to the aspects of intelligent drug delivery and nano medical treatment.

The invention relates to the technical field of sewage treatment, and provides a black and odorous water body combined treatment device which comprises a filtering kettle, a regulating tank, an anaerobic tank, an aerobic tank, a sedimentation tank and a disinfection tank which are sequentially arranged, a first partition plate is arranged in the filtering kettle, and the interior of the filtering kettle is divided into a first chamber and a second chamber by the first partition plate; the filtering kettle is connected with a water inlet pipe and a drain pipe; a second partition plate is arranged in the second cavity, the second partition plate divides the second cavity into two filtering areas, a filtering plate is arranged in each filtering area, and a communicating opening communicating with the first cavity is formed in the top wall of each filtering area; the first chamber is provided with a switching assembly, and the switching assembly is used for switching the flow direction of the sewage, so that the sewage flows into the corresponding filtering areas through different communication ports. According to the combined treatment device for the black and odorous water body, the possibility of blockage of pipelines and equipment can be reduced. The invention further relates to a combined treatment process for the black and odorous water body.

The invention relates to the field of medical information management, in particular to a children malignant tumor treatment process and follow-up visit electronic management system and a children malignant tumor treatment process and follow-up visit electronic management method. The method comprises the steps that the body surface area of a patient is calculated, and the chemotherapy drug dosage in each cycle is calculated based on the body surface area of the patient; performing cycle-by-cycle accumulation on the chemotherapeutic drug dose in each cycle to obtain an actual accumulated dose; calculating and standardizing the specific numerical value of each curative effect index based on the actual accumulated dose, and calculating a comprehensive therapeutic effect score by combining the importance weight of each curative effect index; and calculating the disease-free survival rate based on the actual accumulated dose and the comprehensive treatment effect score. The problems that at present, most medicine dosage calculation methods neglect the special physiological features of children, a treatment monitoring system lacks dynamic adjustment support in the treatment process, a follow-up management system is insufficient in quantitative analysis capacity for long-term prognosis, and personalized long-term health management suggestions cannot be provided for patients are solved.

The invention provides a metal polyphenol network coated self-assembled nano-drug as well as a preparation method and application thereof, and belongs to the technical field of nano-drugs. The self-assembled nano-drug provided by the invention is of an organic-inorganic hybrid core-shell nano-structure; the core of the organic-inorganic hybrid core-shell nano structure is BC NPs; the BC NPs is formed by self-assembly of BBR (Boron Butadiene Rubber) and Cur (Carbon); a shell layer of the organic-inorganic hybrid core-shell nanostructure is a dynamic cross-linked network formed by self-assembly of metal ions and polyphenol through coordination. Metal ions and polyphenol are self-assembled through coordination to form a dynamic cross-linked network, and the dynamic cross-linked network coats the outer layer of the BC NPs nano-particles, so that the BC NPs nano-particles have good biocompatibility and biological safety; and the self-assembled nano-drug shows an obviously enhanced antibacterial effect under the combined treatment of NIR and H2O2.

The invention discloses a sulfur foam combined treatment device and a treatment method thereof, and relates to the technical field of sulfur foam treatment. Comprising a bottom plate, a pair of side plates are fixedly arranged on the two sides of the top face of the bottom plate, a hollow roller is rotationally clamped between the side plates, a center shaft is arranged in the middle of the interior of the hollow roller, and the right side of the center shaft is sequentially and slidably sleeved with a coarse particle filtering disc, a medium particle filtering disc and a fine particle filtering disc; a plurality of groups of first stirring paddles are fixedly arranged on the left side of the fixed sliding shaft, and the right side of the fixed sliding shaft rotationally penetrates through the coarse particle filtering disc, the medium particle filtering disc and the fine particle filtering disc in sequence. In the stirring process, components in sulfur foam are uniform, sulfur particles are fully dispersed, and agglomeration is prevented; during filtering, sulfur particles can be more effectively separated from impurities; after stirring and filtering, the purity of the sulfur product is remarkably improved, and the stability of the quality of the sulfur product is ensured by uniform stirring and fine filtering.

This invention belongs to the field of biomedical technology, specifically disclosing a pharmaceutical composition for treating PIK3CA-mutated colorectal cancer and the application of Alpelisib and Menin-MLL complex inhibitors in combination therapy for colorectal cancer. Experimental results show that the combined use of Alpelisib and Menin-MLL 1 / 2 interaction inhibitors can synergistically inhibit the growth of PIK3CA-mutated colorectal cancer PDX tumors and even induce tumor regression, demonstrating better therapeutic effects than Alpelisib alone. The combination of the two has a synergistic therapeutic effect.

The invention discloses a traditional Chinese medicine composition for promoting blood circulation to remove meridian obstruction and application of the traditional Chinese medicine composition in preparation of a medicine for treating cerebral apoplexy. The traditional Chinese medicine composition is prepared from radix astragali, radix angelicae sinensis, lumbricus, caulis spatholobi, radix salviae miltiorrhizae, flos carthami, ramulus mori, ground beeltle, radix polygoni multiflori preparata, fructus corni, radix morindae officinalis and cortex eucommiae. A novel traditional Chinese medicine formula is obtained by optimizing compatibility on the basis of a traditional old formula, has the effects of tonifying qi, activating blood circulation, tonifying liver and kidney, dispersing blood stasis and dredging collaterals, repairs brain injury caused by cerebral apoplexy and relieves neurological function defect and brain tissue inflammation, is safe and free of drug liver injury toxicity, and is matched with precise medical treatment of western medicine, the treatment effect on cerebral apoplexy is remarkably improved, and the clinical application prospect is wide. Clinical symptoms of patients are improved, neurological function recovery is promoted, and a new Chinese and western medicine combined treatment strategy is provided for clinic.

The invention provides a coordination nano assembly of chiral cyclopeptide and manganese ions as well as a preparation method and application of the coordination nano assembly. According to the method, a manganese superoxide dismutase protein structural domain and a unique tumor microenvironment of melanoma high tyrosinase are combined, and polypeptide sequences L-YD-hL-DD-h, L-YL-HL-DL-H and D-yD-hD-dD-h with different chirality are designed for the first time; the chirality of the polypeptide is regulated and controlled, the in-vivo circulation stability and the cell entry efficiency of the nano assembly are improved, the coordination self-assembly of manganese and different chiral cyclopeptides is realized, and the in-situ oxidation of an in-vivo tumor microenvironment is simulated for photo-thermal therapy and cell membrane coating. The method disclosed by the invention is simple, mild in experimental condition and easy to operate, the prepared cell membrane coated nano assembly not only has long circulation stability and high cell entry efficiency, but also can realize mild photo-thermal therapy through in-situ oxidation, releases manganese ions to activate a CGAS-STING pathway for immunotherapy of tumors, and has a wide application prospect. The potential application value is realized in the field of combined treatment of tumors.

The invention discloses a closed-loop vagus nerve stimulation system acting on depression, which comprises a data acquisition unit, a core analysis unit, a signal regulation and control unit and a nerve stimulation unit, and is characterized in that patient heart rate data and combined treatment data of a depression patient are acquired through the data acquisition unit; the core analysis unit is used for analyzing the influence function of the medicine and electrotherapy combined treatment on the physical signs of the patient so as to obtain an optimal collaborative treatment scheme and a corresponding electrotherapy design scheme, and the signal regulation and control unit is used for carrying out targeted parameter regulation and control on the electrotherapy signal and outputting an electrotherapy optimization signal; personalized electrotherapy is carried out on the depression patient through the nerve stimulation unit, targeted parameter regulation and control are carried out on the closed-loop vagus nerve stimulation system according to the personalized illness state of the patient, and the actual application accuracy of the closed-loop vagus nerve stimulation system is improved by analyzing the combined action mechanism of electrical stimulation and drug therapy. More comprehensive and effective depression auxiliary monitoring and diagnosis and treatment effects are realized.

The invention belongs to the technical field of biological medicines, and particularly relates to application of umbilical cord mesenchymal stem cells and monoclonal antibodies in combined treatment of cancers. The monoclonal antibody is a monoclonal antibody specifically aiming at human PAFAH1B3 protein, the amino acid sequence of a heavy chain variable region of the monoclonal antibody is as shown in SEQ ID NO: 1, and the amino acid sequence of a light chain variable region of the monoclonal antibody is as shown in SEQ ID NO: 2. The PAFAH1B3 protein monoclonal antibody can significantly inhibit the proliferation capacity of pancreatic cancer cells, and after the umbilical cord mesenchymal stem cells and the PAFAH1B3 protein monoclonal antibody are combined for use, the inhibition effect on the proliferation capacity of the pancreatic cancer cells is better, and the umbilical cord mesenchymal stem cells can be used for preparing anti-cancer drugs and have good application prospects.

The invention aims to provide a preparation method and application of a metal-organic nano complex for combined treatment of tumors, belongs to the technical field of antitumor drugs, and adopts a one-pot method to prepare copper-quercetin nanoparticles with uniform morphology. The obtained metal organic complex CQT nanoparticles can be used for X-ray induced sensitization radiotherapy / chemical power combined treatment to promote tumor cell apoptosis, meanwhile, due to introduction of copper ions, the steady state of the copper ions in tumor cells is broken, tumor cell copper death is induced, interaction is formed between copper death and the radiotherapy biological effect, and the tumor cell apoptosis is promoted. The synergistic tumor treatment effect is realized. The novel CQT nano complex for three-in-one treatment of sensitization radiotherapy / chemodynamic therapy / copper death has the function of obviously delaying the growth speed of tumors. The system can maintain good stability and biocompatibility, provides an innovative and efficient treatment potential scheme for intractable tumors clinically lacking specific targets, and improves the long-term prognosis of early metastatic tumors.

The invention provides a microneedle preparation for co-delivering photo-thermal nano-enzyme and elemene as well as a preparation method and application of the microneedle preparation. The preparation method comprises the following steps: firstly, preparing a novel photo-thermal nano enzyme Au (at) MoS2 through a two-step water phase synthesis strategy; benefited from the synergistic effect of the hybrid material, the nano-enzyme shows significantly enhanced near-infrared (NIR) photothermal effect, peroxidase-like (POD) activity and glutathione-like peroxidase (GSHOx) activity, and can effectively remodel the tumor microenvironment and destroy the redox steady state. Furthermore, photo-thermal nano enzyme, beta-ELE and hyaluronic acid are used as raw materials, and a novel soluble microneedle preparation is successfully prepared through a template method. The novel composite microneedle is in a sharp rectangular pyramid shape, growth of melanoma can be effectively inhibited through percutaneous delivery of photo-thermal nano-enzyme and beta-ELE, remarkable systemic toxicity is avoided, and an innovative strategy is provided for non-invasive, efficient and safe melanoma combined treatment.

The invention discloses an application of a nano-drug and combined radiotherapy in cancer treatment, and relates to the technical field of nanotechnology and biomedicine. The nano-drug (GL) obtained by the simple preparation method is a gambogic acid loaded nano-liposome, and the GL has good biocompatibility and passive targeting property. According to the invention, GL and FLASH radiotherapy are combined to treat cancers, and through the synergistic effect of GL and FLASH radiotherapy, double striking on tumor cells is realized, so that the anti-tumor effect is remarkably enhanced, side effects in the treatment process are reduced, and the life quality of patients is improved. Besides, the combined treatment strategy effectively overcomes the problem that cancer is not sensitive to existing radiotherapy and chemotherapy, more remarkable and efficient anti-tumor effect is achieved, more treatment choices and hopes are brought to cancer patients, and a new way is opened up for cancer treatment.

The invention provides a tetrahedral framework multifunctional nano-composite as well as a preparation method and application thereof, and belongs to the field of nucleic acid molecule medicines. According to the invention, micromolecule drug loading is realized on a miRNA delivery system based on tetrahedral framework DNA for the first time, the miRNA and antibiotic co-delivery nano-composite is prepared, and the nano-composite has high stability, good cell and tissue permeability and remarkable biocompatibility, can be combined with anti-inflammatory action and antibacterial activity at the same time, and can be used for preparing the nano-composite for co-delivery of the miRNA and the antibiotic. The compound is applied to combined treatment of infected wound healing, and a new treatment strategy is provided for treating complex and refractory diseases.

The invention relates to a preparation method of a lophatherum gracile extract as well as a product and application of the lophatherum gracile extract. The preparation method comprises the following steps: mixing a lophatherum gracile raw material with an ethanol water solution, then carrying out microwave-ultrasonic combined treatment, then carrying out reflux extraction, carrying out vacuum concentration on an extracting solution, and carrying out purification and elution on a concentrated solution by adopting macroporous resin to obtain the lophatherum gracile extract. And carrying out flocculation treatment on the eluent, and then drying to obtain the lophatherum gracile extract. The extract prepared by the preparation method is clear in effective components, has excellent DPPH free radical scavenging capacity and prostaglandin and capsaicin receptor inhibition capacity, is free of pesticide residues, can be used as an active component of skin care products and medical auxiliary supplies, and has the effects of resisting oxidation, resisting inflammation and relieving.

The invention discloses poplar and phellinus igniarius polysaccharide SVP-1 as well as a preparation method and application thereof, and belongs to the field of biological medicines. The poplar phellinus igniarius polysaccharide SVP-1 provided by the invention has remarkable anti-tumor activity, and can effectively inhibit proliferation, invasion and migration of tumor cells so as to block malignant progression of tumors. The structure is clear, the preparation method is controllable, the stability and biological activity of polysaccharide components are ensured, and a reliable basis is provided for development of antitumor drugs. When the traditional Chinese medicine composition is used together with radiotherapy and chemotherapy medicines, a synergistic effect can be achieved, the sensitivity of tumors to treatment can be improved, the toxic and side effects of radiotherapy and chemotherapy can be relieved, and the effects of reducing toxicity and increasing efficiency are achieved. According to the combined treatment strategy, the curative effect of the existing tumor therapy can be remarkably improved, meanwhile, adverse reactions are reduced, and a new optimization scheme is provided for clinical tumor treatment.

The invention discloses an aggregation-induced emission near-infrared photothermal molecule as well as a preparation method and application of the aggregation-induced emission near-infrared photothermal molecule, and belongs to the technical field of biomedicine. The invention provides the preparation method of the aggregation-induced emission near-infrared photothermal molecule, the synthesis process is simple and efficient, and the obtained novel near-infrared photothermal molecule has better luminescence performance or photothermal performance of a near-infrared second region. Meanwhile, the near-infrared aggregation-induced emission protein nanoparticles provided by the invention are simple in preparation process and excellent in performance, and can effectively cross nasal mucosa and target an A beta plaque part, so that AD visualization and photo-thermal / hypoxia relief combined treatment are realized.

The present invention relates to: a temperature-sensitive hydrogel for cancer treatment; a photothermal composition comprising the hydrogel as an active ingredient; and a preparation method for the temperature-sensitive hydrogel for cancer treatment. The temperature-sensitive hydrogel of the present invention includes gold nanostars as active ingredients, and thus can generate heat by light irradiation, thereby exhibiting a photothermal therapy effect. In addition, the hydrogel temperature increases so that the release of nitric oxide (NO) from S-nitrosocysteine can be induced. In addition, the temperature-sensitive hydrogel of the present invention includes S-nitrosocysteine as an active ingredient so that, upon a temperature rise due to a photothermal reaction, the penetration of drugs into a tumor site can be improved by the release of NO, and at the same time, apoptosis of cancer cells can be directly caused; and includes an immunotherapy agent as an active ingredient so that tumor size can be effectively suppressed even with the passage of time. Therefore, as a material that enables complex treatment combined with photothermal therapy and immunotherapy, the temperature-sensitive hydrogel of the present invention having the aforementioned effect can be usefully utilized in the field of medicine for cancer treatment.

The utility model discloses a comprehensive treatment table suitable for left-hand and right-hand doctors, and relates to the technical field of medical instruments. The comprehensive treatment table suitable for the left-hand and right-hand doctors comprises a bottom plate, a left-hand support plate and a right-hand support plate, the case is mounted on one side of the top of the bottom plate; the spittoon is mounted at the top of the case, and the bottom of the spittoon is rotationally connected with the case; the cushion assembly is connected to one side of the case and used for supporting the head and the body of a patient; the lifting table is connected to one side of the case, and the cushion assembly is rotationally connected with the lifting table; the driving assembly is arranged on the lifting table and used for driving the cushion assembly to rotate; the cantilever assembly is rotationally connected to the bottom plate; and the control assembly is rotationally connected to the top of the case.

The invention relates to a nano assembly for self-synergistically enhancing ferroptosis as well as a preparation method and application of the nano assembly, and belongs to the technical field of pharmaceutical preparation combined treatment of new auxiliary materials and new dosage forms. The invention firstly provides a functional conjugate linker capable of automatically generating ROS, the functional conjugate linker comprises artesunate-ferrocene or dihydroartemisinin-ferrocene, and the structural formulas of the functional conjugate linker and the artesunate-ferrocene or dihydroartemisinin-ferrocene are respectively shown as a and b. According to the present invention, the ROS self-generating functional conjugate linker and the ferroptosis inducer are co-assembled to form the nanometer assembly, such that the ROS level in the tumor cells can be effectively and synergistically improved by the ROS self-generating functional conjugate linker and the ferroptosis inducer so as to cause the tumor cell lipid peroxidation storm, and the ferroptosis induction effect can be significantly improved. The co-assembled nano preparation provided by the invention provides a new strategy and more choices for developing the delivery of drugs, and meets the urgent requirements of efficient and diversified ferroptosis-based cancer treatment strategies on preparations in clinic. # imgabs0 #

The utility model provides combined treatment equipment for high-salinity industrial wastewater, which comprises a filter box body and a cover plate arranged on the filter box body, two filter plates are arranged in the filter box body, and the two filter plates are clamped in slots formed in the cover plate and clamping grooves formed in the filter box body through clamping components. When the filter plate needs to be detached for replacement, cleaning or maintenance, a knob is firstly pressed downwards, the knob exerts pressure on a first spring, a clamping plate slides out of a first clamping groove and a second clamping groove, and at the moment, the knob is rotated to drive the clamping plate to move in a first mounting groove and a second mounting groove until the clamping plate slides out of the second mounting groove; at the moment, the clamping plate does not limit the filter plate, the filter plate can be pulled out from the inserting groove of the cover plate and the clamping groove in the filter box body, and then the filter plate is disassembled, the disassembly mode is time-saving and labor-saving, maintenance personnel do not need to disassemble the whole filter box body, the maintenance process is greatly simplified, the operation efficiency is improved, and the manpower and time cost is reduced.

The invention relates to a combined traditional Chinese medicine composition for treating tumors by improving a tumor immune microenvironment and application thereof. The combined traditional Chinese medicine composition comprises rhein and hesperidin. Rheinic acid and hesperidin have stronger antitumor activity than single components in combined application, and the combined pharmaceutical composition can significantly inhibit the growth of cancer-related fibroblasts, reduce CCL2 secreted by cancer-related adipocytes and increase infiltration of CD8 + T cells at tumor sites, so that the growth of tumors is significantly inhibited, and the antitumor effect is improved. And a new method and thought are provided for combined treatment of cancers.

The invention provides a multifunctional microsphere for tumor chemoradiotherapy embolism treatment and imaging and a preparation method and application thereof.According to the multifunctional microsphere, a polylactic acid-glycolic acid copolymer serves as a framework material, a small molecule medicine is entrapped in the multifunctional microsphere, and surface modification and radionuclide labeling are conducted at the same time; the small molecular medicine is prepared from one or more of icaritin, icariin, lecimod, imiquimod, motod, visamod and tilamod, and preferably, the icaritin and the lecimod are selected; the surface modification is performed by adopting tyramine or tyrosine polypeptide; the radionuclides include 123I, 125I, and 131 I, preferably 131 I. The preparation process and equipment of the multifunctional microsphere are simple, the cost is low, chemotherapy drugs and immunologic adjuvants are entrapped at the same time, radionuclides are marked, hepatic artery embolism treatment, 131I long-term local nuclide treatment and combined treatment of local chemotherapy and immunoregulation are achieved, and meanwhile the SPECT / CT imaging in-vivo tracing function of the multifunctional microsphere is achieved.

The invention belongs to the technical field of biological medicine, and particularly relates to application of hUC-MSCs combined with Met in preparation of anti-diabetic drugs. The diabetes mellitus is particularly type 2 diabetes mellitus. Through construction of a T2DM rat model, paraffin tissue section detection, serum ELISA, cell experiments and the like, researches prove that T2DM can be effectively treated by HUC-MSCs combined with Met treatment, including reduction of body blood sugar and recovery of blood sugar regulation ability; liver injury caused by T2DM is relieved and repaired; the glycolipid metabolism of the liver of the T2DM rat is improved; the immune inflammation disorder caused by T2DM is relieved; and the apoptosis level of liver cells is improved. And compared with a single hUC-MSCs or Met treatment mode, the combined treatment mode has a better treatment effect. The invention provides a theoretical basis and a new treatment strategy for treatment of T2DM.

The invention relates to a burn treatment path planning technology, and discloses a burn treatment optimal massage path planning method based on a graph theory and an SAC algorithm. The system comprises a data acquisition and initialization module, a path modeling module, a reinforcement learning module, an optimization and generation module and a closed-loop management module. The data acquisition and initialization module is used for generating accurate point cloud data through a binocular camera in combination with an IMU (Inertial Measurement Unit), and calculating burn average degree and uniformity to provide a basis for subsequent processing; the path modeling module adopts a graph theory method to model a burn area into nodes and edges, and multi-objective optimization is carried out by integrating treatment efficiency and patient comfort; the reinforcement learning module designs a state space, an action space and a reward function based on an SAC algorithm, and realizes optimization of a path and a strength strategy; the optimization and generation module generates a final treatment path through initial exploration and local optimization, and introduces an adaptive force adjustment function to prevent secondary injury; the closed-loop management module supports off-line learning and real-time feedback, and ensures high efficiency and individuation of a therapeutic scheme through dynamic parameter adjustment and report generation. According to the method, the accuracy and efficiency of burn treatment are effectively improved, and the method has wide clinical application potential.

The invention provides a metal iridium complex with a porphyrin part for multi-mode thrombolysis, a preparation method, nanoparticles and application, and belongs to the technical field of chemical synthesis and biomedicine. The structural formula of the metal iridium complex is shown as a formula 1 or a formula 2. The invention also provides a preparation method of the metal iridium complex, and also provides a nano particle which is prepared from the metal iridium complex with a porphyrin part for multi-mode thrombolysis as shown in a formula 1 or a formula 2. The invention also provides application of the nanoparticles as a medicine in preparation of medicines for treating thrombosis diseases. The metal iridium complex disclosed by the invention not only can realize PTT / SDT dual-mode combined treatment of thrombus, but also can be used as a chemiluminescent substrate to act with active oxygen for imaging, so that integration of diagnosis and treatment is realized.

The invention discloses a pharmaceutical composition for treating hashimoto thyroiditis, and belongs to the technical field of traditional Chinese medicines. According to the pharmaceutical composition, astragalus membranaceus and codonopsis pilosula are used as monarch drugs to tonify qi, raise yang, tonify qi and strengthen body resistance; radix bupleuri, rhizoma cyperi and radix curcumae are used as ministerial drugs for soothing liver-qi stagnation, expelling evil, soothing liver-qi, regulating qi and harmonizing blood; humifuse euphorbia herb, lucid ganoderma and oldenlandia diffusa are used as adjuvant drugs for clearing away heat and toxic materials, reinforcing qi, nourishing blood and tranquilizing; the whole formula has the effects of tonifying qi, soothing the liver and clearing heat together. All the medicines are combined to effectively improve the symptoms of patients with hashimoto thyroiditis and reduce the thyroid autoantibody level. In addition, the pharmaceutical composition is good in safety, and a traditional Chinese and western medicine combined treatment strategy for hashimoto thyroiditis can be further popularized in the future.

The invention discloses an oxygen-enhanced fluorinated nano assembly for bidirectional amplification of tumor ferroptosis as well as a preparation method and application of the oxygen-enhanced fluorinated nano assembly, and belongs to the technical field of pharmaceutical preparation combined treatment of new auxiliary materials and new dosage forms. According to the invention, the oxygen-functionalized fluorinated adriamycin prodrug with oxygen carrying capacity is designed and synthesized for the first time, a hybrid nano-assembly composed of the disulfide-bridged fluorinated adriamycin prodrug and the ferroptosis inducer is constructed and can be used for tumor treatment, and a fluorinated functional side chain can efficiently carry oxygen to break tumor hypoxia trapping and relieve limitation of hypoxia on Sor, so that the drug can be applied to tumor treatment. DOX can activate NOXs and promote generation of ROS through supplemented oxygen as a catalytic substrate, redox balance is broken through in a two-way synergistic mode, excessive accumulation of lipid peroxide is caused, and therefore the ferroptosis treatment effect is enhanced, a new strategy and more choices are provided for drug delivery development, and the application prospect is wide. The urgent demand of the hypoxia solid tumor on efficient and diverse ferroptosis treatment strategies on preparations in clinic is met.

The invention discloses an anti-tumor combined pharmaceutical composition and application thereof, and belongs to the technical field of biological medicine. The combined pharmaceutical composition comprises an OTUD4 inhibitor and a ferroptosis inducer or regorafenib which serve as active ingredients, and the OTUD4 inhibitor inhibits expression of OTUD4 in a targeted mode or causes function deficiency of OTUD4 and promotes occurrence of ferroptosis in tumor cells. The combined pharmaceutical composition provided by the invention more effectively inhibits tumor growth by synergistically increasing the oxidative stress level in cells, thereby playing a stronger anti-tumor effect. The combined treatment scheme provided by the invention provides a new thought for tumor treatment, and particularly in patients ineffective or drug-resistant to single-drug treatment, targeted inhibition of OTUD4 and regorafenib can effectively overcome drug resistance and significantly inhibit tumor growth.

The invention discloses a lung cancer PDL1 immunotherapy drug-resistant cell strain LLC-R and application thereof, and relates to the technical field of cell biology. The cell strain LLC-R is preserved in the China Center for Type Culture Collection on November 6, 2024, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: C2024374. The invention provides a reliable experimental model for researching the drug resistance mechanism of lung cancer on PDL1 immunotherapy and related signal channels, so that technicians in the field can more deeply understand the reaction of lung cancer cells on PDL1 immunotherapy, which is helpful for exploring new biomarkers and researching and developing new combined treatment strategies, and has a wide application prospect. The treatment effect is improved; the occurrence of drug resistance is reduced. The invention has important scientific and clinical significance for deeply understanding the lung cancer PDL1 immunotherapy drug resistance and improving the clinical treatment effect of patients.