37 results about "Oxygen-carrying" patented technology

The invention provides an oxygen-carrying protein molecule and a preparation method and application thereof, and relates to the technical field of functional protein preparation, the oxygen-carrying protein molecule comprises at least one cysteine part and at least one histidine part, the cysteine part comprises acetyl or ethyl maleoyl, and the histidine part comprises histidine. The histidine moiety includes a benzimidazole derivative group. The benzimidazole derivative is adopted as an auxiliary molecule and participates in the modification process of the sulfydryl modified compound on the hemoglobin, the modification site selectivity is improved, the conformational change is reduced, and the modification degree is accurately controlled, so that the oxygen binding force P50 of the modified hemoglobin is effectively improved.

The invention discloses a disposable isolated organ continuous oxygenation mechanical perfusion circulation system and a working method. A circulation pipeline of the circulation system is provided with a to-be-carried oxygen conveying pipeline and an oxygen-carrying perfusion pipeline; the output end of the organ low-temperature storage container is connected to the to-be-carried oxygen conveying pipeline, and the pump pipe and the oxygenator are arranged on the to-be-carried oxygen conveying pipeline; the organ transfer sleeve assembly is detachably mounted on the organ tray assembly; the organ tray assembly is arranged in a cavity of the organ low-temperature storage container; the output end of the oxygenator is connected to the oxygen-carrying perfusion pipeline, the automatic bubble separator installed on the oxygen-carrying perfusion pipeline is connected to the input end of the organ transfer sleeve assembly, and the pressure sensor is installed on the oxygen-carrying perfusion pipeline. The device has the following advantages: the device has an automatic bubble removal function; on the basis of a low-temperature mechanical perfusion function, the oxygenation function is achieved; light weight is taken into account, and portable transfer of perfusion equipment is facilitated; the method has higher safety and reliability.

The invention discloses a preparation method of metal organic framework armored red blood cells and an application of the red blood cells in cryopreservation, an MOF armor coated on the surfaces of the red blood cells in situ in one step is prepared in a physiological environment, the red blood cells can be effectively prevented from being damaged by freezing induction, and meanwhile, the natural oxygen carrying function of the red blood cells is kept. The unique ice control capacity of the MOF armored red blood cells is explored, including inhibition of recrystallization of ice, induction of smoothness of the ice front, reduction of formation of sodium chloride eutectic and acceleration of ice melting, a novel design strategy is provided for an MOF-based ice control material, and the future prospect of the MOF-based ice control material in the field of efficient ice inhibition is possibly widened.

The present invention discloses a functional solid beverage for resisting altitude sickness and a preparation method thereof. The raw materials of the solid beverage include yak spleen small molecule polypeptide, yak milk small molecule polypeptide, taurine, phosphatidylserine, crystalline fructose and flaxseed; wherein the amino acid sequence of the yak spleen small molecule polypeptide is shown as SEQ ID NO.1, and the amino acid sequence of the yak milk small molecule polypeptide is shown as SEQ ID NO.2. The functional solid beverage of the present invention promotes oxygen carrying and improves the oxygen transport efficiency of red blood cells by reducing the deformation and adhesion of red blood cells caused by plateau exposure, thereby effectively preventing and alleviating altitude sickness such as headache, nausea, fatigue, etc. The product is portable and easy to use, the ingredients are safe and harmless, and the effect has been verified to be significant through experiments. The functional solid beverage of the present invention provides scientific and effective health protection for people entering high-altitude areas, and has broad market prospects and application value.

The invention discloses a traditional Chinese medicine composition and a traditional Chinese medicine preparation for treating sleep disorder caused by new coronavirus infection and a preparation method thereof, and belongs to the technical field of traditional Chinese medicines. Aiming at the problems that no medicine with definite curative effect exists for sleep disorder of a new coronavirus infected patient at present, and the medicine can only depend on the individual immunity of the patient to resist, the traditional Chinese medicine composition comprises the following traditional Chinese medicinal materials in parts by weight: 10g of radix pseudostellariae, 12g of radix paeoniae rubra, 10g of radix salviae miltiorrhizae, 6g of rhodiola rosea, 6g of exocarpium citri grandis, 5g of ginger processed pinellia, 15g of poria cocos, 15g of poria with hostwood, 15g of herba patriniae, 15g of tuber fleeceflower stem, 12g of hairyvein agrimony, 6g of liquorice, 10g of chrysanthemum and 6g of cassia twig. The traditional Chinese medicine composition is based on the principles of tonifying qi and activating blood, reducing phlegm and dredging collaterals, and clearing pestilence and soothing nerves, all the medicines are synergistic and supplement one another, the blood oxygen concentration and the red blood cell oxygen carrying capacity of a human body are improved, the blood viscosity is reduced, microcirculation is promoted, residual toxins are eliminated, and patients are helped to recover high-quality sleep.

The utility model provides a portable low-temperature oxygen-carrying organ transfer box which comprises a machine shell and a cover plate, a miniature oxygen generator is installed on the machine shell, two sets of air outlet connectors are arranged on the machine shell, and the miniature oxygen generator is connected with the two sets of air outlet connectors through connecting pipes. The cover plate is rotatably connected with the shell to form a placement cavity, a storage assembly is arranged in the placement cavity, the storage assembly comprises a covering shell, a container bag and an oxygenator are arranged in the covering shell, two groups of oxygen circulation openings are formed in the container bag, one group of air outlet connectors are connected with the oxygenator, and the other group of air outlet connectors are connected with one group of oxygen circulation openings; a control panel is arranged on the machine shell, and a power supply and a main control board are installed on one side of the machine shell. According to the transfer box, the miniature oxygen generator is installed on the machine shell, the miniature oxygen generator is communicated with the oxygenator and the container bag in the storage assembly through the connecting pipe, oxygen can be supplied to organs at low temperature, accumulation of toxic metabolites of the organs is effectively avoided, and the risk of ischemia reperfusion injury is reduced.

The invention discloses a method for removing methemoglobin in a hemoglobin solution. The method comprises the following steps: firstly, adjusting the pH value of the hemoglobin solution to 6.5-8.5 and the ion strength to 0-3000mmol / L; deoxidizing the hemoglobin solution to obtain a deoxidized hemoglobin solution; raising the temperature of the deoxidized hemoglobin solution to a certain temperature and maintaining the temperature for a certain time; and finally, removing suspended or precipitated substances in the solution to obtain the hemoglobin solution for removing the methemoglobin. The process steps are pure physical processes, and the MetHb component can be effectively removed on the premise that new impurities or new components are not brought into an original system; the hemoglobin structure and oxygen carrying and releasing performance are not obviously influenced, the technological process is simpler than that of a chemical method, and the cost is low. In addition, the treatment process is easy to control, the content of Hb in the solution is not affected by the process, and the property is stable. The whole adopted temperature range is similar to the temperature condition of the existing biological product pasteurization virus inactivation process, and the method has the possibility of being combined with the virus inactivation process and has good application value.

The invention discloses a low-oxygen affinity deoxidized hemoglobin spray and application thereof. The invention creatively discovers that the oxygen concentration on the surface of a wound is obviously higher than that of pure deoxidized hemoglobin and high-oxygen-affinity deoxidized hemoglobin with higher oxygen carrying capacity after the low-oxygen-affinity deoxidized hemoglobin with higher oxygen release capacity is used; it is proved that the low-oxygen affinity deoxidized hemoglobin is more beneficial to improvement of the oxygen concentration of the wound surface. According to the specific embodiment of the invention, the RSR-13 is combined with the deoxidized hemoglobin, the conformation of the deoxidized hemoglobin is changed, the oxygen affinity of the deoxidized hemoglobin is reduced, the oxygen release of the deoxidized hemoglobin in the hypoxia tissue is effectively enhanced, the drug has no obvious toxicity to skin cells, the effectiveness and safety of the drug in skin wound treatment are improved, and the application prospect is wide. The method is suitable for treating multiple types of wound surfaces, and has wide application value and market prospect.

The utility model provides an oxygen-carrying perfusion device. The oxygen-carrying perfusion device specifically comprises a kidney perfusion operation box and a portable oxygen generation unit. The kidney perfusion operation box is additionally provided with an oxygen adjusting unit, and the oxygen adjusting unit comprises an oxygen adjusting control panel, an oxygen flow adjusting valve, an oxygen flow sensor and an oxygen concentration sensor. The portable oxygen generation unit comprises an oxygen generation device control panel and an oxygen generation device, an output pipeline of the oxygen generation device is communicated with an oxygen flow regulating valve, a user inputs an instruction from a control display of the kidney perfusion operation box, and a main control panel of the kidney perfusion operation box is communicated with the oxygen generation device control panel and an oxygen regulating control panel. The oxygen adjusting control panel and the oxygen generating device control panel control the opening size of the oxygen flow adjusting valve and the output duty ratio of a compressor of the oxygen generating device to achieve control over the flow and concentration of oxygen input by the displayer. By means of the structure, a user can adjust oxygen supply parameters in real time according to requirements, and therefore convenient and flexible kidney oxygen-carrying perfusion is achieved.

The invention relates to a hemoglobin oxygen carrier based on small molecule modification as well as a preparation method and application thereof. The hemoglobin oxygen carrier comprises hemoglobin and N-fluoromethoxycarbonyl diphenylalanine, the hemoglobin forms the hemoglobin oxygen-carrying carrier through the non-covalent bond acting force between the hemoglobin and the N-fluoromethoxycarbonyl diphenylalanine. According to the hemoglobin oxygen carrier disclosed by the invention, a co-assembly strategy of N-fluoromethoxycarbonyl diphenylalanine and hemoglobin is rapidly implemented by taking a non-covalent bond acting force as a driving force. The hemoglobin oxygen-carrying carrier prepared by the process greatly shortens the stabilization time of hemoglobin molecules on the basis of ensuring the stability of molecular chemical groups, has good biocompatibility and injectable application characteristics on the basis of the characteristics of non-covalent acting force, and provides a new foundation for research and development of a new generation of hemoglobin oxygen-carrying carriers. And an engineering design thought and a theoretical basis are provided.

The invention discloses a preparation method of hydrogel with oxygen carrying and photo-thermal functions. The preparation method comprises the following steps: 1, dissolving chitosan, adding dopamine and hemoglobin, continuously stirring, and freeze-drying to obtain a hydrogel precursor; 2, dissolving the hydrogel precursor in deionized water or a phosphate buffer solution, adding dialdehyde polyethylene glycol, uniformly stirring, and standing to obtain hydrogel; the invention also discloses a hydrogel with oxygen carrying and photo-thermal functions. Chitosan, dopamine and hemoglobin are adopted as raw materials to prepare a hydrogel precursor, reversible imine bonds are formed through a Schiff base reaction between polydopamine and chitosan, covalent bonds are cross-linked through a Michael addition reaction, and the hydrogel precursor is synthesized through the complexing effect of polydopamine-hemoglobin; and then mixing the hydrogel precursor with dialdehyde polyethylene glycol to form the hydrogel applied to diabetes wound treatment. The invention is applicable to the technical field of polymer materials.

The present application relates to a kind of from starworm separates oxygen-carrying active haemerythrin method, belong to protein separation and purification technical field.This method is first obtained from the body cavity fluid of square starworm pigment cell, obtains crude haemerythrin liquid after lysis, haemerythrin molecule is separated by multistage tangential flow ultrafiltration technology, further by vacuum low-temperature freeze-drying method reaches the purpose of long time keeping active.This application obtains body cavity fluid by cutting short starworm end, and almost does not affect its edible value, is selectively lysed pigment cell by configuring hypotonic lysis solution and protects haemerythrin activity, determines multistage tangential flow ultrafiltration membrane pore size specification and use order and other key parameters, provides freeze-dried powder preparation method to long time maintain activity.The present application discloses a kind of from starworm high-efficiency separation active haemerythrin method complete technical scheme, is suitable for biological medicine field haemerythrin research and development and starworm industry intensive processing.

The invention discloses a multispectral response oxygen-carrying artificial periosteum which comprises an inner layer oxygen-carrying module and an outer layer control module controlled by outer layer response light. The preparation method of the artificial periosteum comprises the following steps: 1, preparing an inner core solution and a shell solution; 2, injecting the inner core solution and the shell solution into coaxial electrostatic spinning equipment to prepare the inner layer oxygen carrying module; 3, preparing a dopamine solution; and 4, soaking the inner layer oxygen carrying module in the dopamine solution to prepare the artificial periosteum. The problem that in the prior art, a tissue engineering periosteum cannot supply oxygen locally is solved; the aim of remotely adjusting the oxygen release speed through non-invasive intervention is achieved, oxygen is supplied according to the hypoxia tolerance characteristics of the stem cells, and the survival rate of the early stem cells is increased; a'sequential oxygen supply 'strategy is provided, that is, endogenous oxygen is provided for the damaged tissue microenvironment after exogenous oxygen is exhausted through early vascularization, so that the oxygen level of the microenvironment is maintained. Finally, the stem cell fate is regulated and controlled, and tissue regeneration and functional recovery are accelerated.

The invention relates to the field of gas breathing circulation regeneration, and discloses a gas breathing circulation system based on bionic half gills and an underwater respirator, the gas breathing circulation system comprises a respirator, the respirator is used for breathing interaction of a user; the bionic half gill is internally provided with a permeable diaphragm for air-water exchange; the respirator and the bionic half gill are connected in series through a connecting pipeline to form a gas circulation loop; based on the characteristic that the solubility and the mass transfer rate of carbon dioxide in water are far higher than those of oxygen, the scheme realizes that carbon dioxide is efficiently transferred only by a physical diaphragm without a chemical adsorbent, so that not only is the chemical risk thoroughly eliminated, but also the utilization rate of oxygen is increased by several times by recycling residual oxygen in exhaled gas; and finally, the underwater operation time is remarkably prolonged under the same oxygen carrying amount, meanwhile, the breathing comfort is guaranteed through the gas adjusting cavity, intelligent regulation and control of gas components are achieved through the exhaust port, and the system has safety, high efficiency and engineering feasibility.

The invention provides an oxygen-carrying synergist, a preparation method thereof and application of the oxygen-carrying synergist in underground supercritical water coal gasification. The preparation method comprises the following steps: mixing alkali carbonate, hydrogen peroxide and a compound stabilizer to form a first system, and controlling the pH value of the first system to be 7-9; adding the first system into a dispersion liquid containing siloxane, a water-in-oil emulsifier and a dispersant solution, and mixing to form a second system; adding a curing agent into the second system for curing reaction to form a third system; the third system is subjected to freeze-drying treatment, and the oxygen-carrying synergist is obtained. When the obtained oxygen-carrying synergist is subsequently applied to underground supercritical water coal gasification, the effective utilization rate of coal and hydrogen selectivity can be effectively improved, and the metal corrosion risk is reduced.

The invention relates to the technical field of hylocereus undatus moxibustion, in particular to a formula of hylocereus undatus moxibustion as well as a preparation method and application of the hylocereus undatus moxibustion, and the hylocereus undatus moxibustion is prepared from the following raw materials in parts by weight: 35-40 parts of folium artemisiae argyi, 5-10 parts of a modified carbon agent, 4-7 parts of a graphene regulator and 8-12 parts of an auxiliary agent; wherein the adjuvant is prepared from the following raw materials in parts by weight: 15-20 parts of fresh ginger, 5-10 parts of radix angelicae sinensis, 5-10 parts of cortex cinnamomi and 8-12 parts of cortex eucommiae. The hylocereus undatus moxibustion is beneficial to the human body; qi and blood circulation can be quickly recovered, primordial qi can be replenished, Qi and blood running channels (oxygen carrying capacity of red blood cells) can be opened, and anemia can be improved; the self-immune function and the activity of white blood cells are rapidly improved; the tissue blood supply is increased, and the sub-health state is improved.

The present invention discloses a fluorinated zinc phthalocyanine derivative and its preparation method and application. The fluorinated zinc phthalocyanine derivative is obtained by chemically covalently linking trithiocarbonate to the four amino groups of tetraaminoperfluorophthalocyanine zinc. The fluorinated zinc phthalocyanine derivative can be used as a star-shaped RAFT chain transfer agent to induce hydrophobic methacrylate monomers and / or hydrophilic acrylate monomers to prepare four-arm star-shaped random copolymers through near-infrared light-controlled reversible addition-fragmentation chain transfer polymerization. The star-shaped polymer prepared by the above method has a narrow molecular weight distribution and can form polymer micelles with uniform hydrodynamic diameter in an aqueous solution below its critical aggregation concentration. In addition, the above polymer micelles have a high singlet state yield and good oxygen carrying capacity, and can be used for two-photon photodynamic therapy of hypoxic tumors.

The invention relates to the technical field of animal feed, in particular to application of rhodiola rosea in introduction of Berkshire pigs. The invention relates to an application of rhodiola rosea in introducing Berkshire pigs to improve the blood oxygen carrying capacity of the Berkshire pigs and / or protect the liver to enhance the immunity and / or reduce the feed-gain ratio to improve the feed conversion rate. The method has the advantages that (1) the feed-to-gain ratio is reduced, and the feed conversion rate is improved; and (2) the blood oxygen carrying capacity of the Berkshire pigs is improved. And (3) liver protection and immunity enhancement. And (4) excessive fat deposition is avoided. And (5) the richness of harmful bacteria is reduced. And (6) increasing the generation of beneficial metabolites.

The present invention discloses a fluorinated tin phthalocyanine derivative, its preparation method, and application. The fluorinated tin phthalocyanine derivative is prepared by chemically covalently linking a perfluorocarbon to a tin phthalocyanine side chain. Compared with tin phthalocyanine, the fluorinated tin phthalocyanine derivative not only has an oxygen-carrying function but also effectively improves the singlet oxygen yield and photostability of tin phthalocyanine. The present invention also provides a nanophotosensitizer. The fluorinated tin phthalocyanine derivative is PEGylated to form a PEGylated fluorinated tin phthalocyanine derivative that can self-assemble in water to form nanoparticles. The nanophotosensitizer exhibits excellent performance in terms of singlet oxygen yield, photostability, solution stability, and oxygen carrying capacity, and has good application prospects in photodynamic tumor therapy.

The present invention discloses a carbon-deposition-resistant perovskite oxygen carrier for methane chemical chaining to produce synthesis gas, as well as its preparation method and application. The preparation method of the carbon-deposition-resistant perovskite oxygen carrier comprises the following steps: (1) dissolving lanthanum nitrate and ferric nitrate in water, adding glycine as a sol-gelling agent, and adjusting the pH value of the solution to 1.4-1.85 to obtain a precursor solution; (2) heating and stirring the precursor solution to evaporate the water to form an oily wet gel, and drying and activating it to obtain an activated precursor; (3) calcining the activated precursor at 500-900°C for 2-6 hours to obtain a LaFeO3 oxygen carrier, namely the carbon-deposition-resistant perovskite oxygen carrier for methane chemical chaining to produce synthesis gas. The method of the present invention can increase the specific surface area, oxygen carrying capacity and catalytic properties of the oxygen carrier, can convert methane into H2 and CO, and reduce carbon deposition.

The application provides a porous catalytic hydrogel with high enzyme loading and a preparation method and application thereof, the nanoparticle immobilized enzyme contained in the porous hydrogel enhances the enzyme immobilization effect, plays a certain leakage prevention effect, and prolongs the service life; meanwhile, the hydrogel has the catalytic property and oxygen carrying property of the nanoparticle immobilized enzyme and certain viscoelasticity, has good biocompatibility, and is more convenient to adhere and replace with skin and other tissues; the porous catalytic hydrogel is gelled in a thermal polymerization mode, and has the advantages of simplicity and rapidness; the prepared hydrogel has a reticular structure inside, has a high specific surface area, and the preparation method can adjust the pore size of the hydrogel by adjusting the use ratio of the carboxymethylated cellulose nanofiber and betaine, so that the hydrogel matched with the higher enzyme loading is obtained, the hydrogel has higher catalytic capacity, and has wide application prospects in electrochemical sensors.

The invention discloses a blood stabilizer, a blood bag and a preparation method of the blood stabilizer, the blood stabilizer is a polymeric monomer or an oligomer of the polymeric monomer, and the polymeric monomer is composed of ethylene glycol, propylene glycol and glycerol according to the molar ratio of 1: 1: (0.5-2). The invention discloses a blood stabilizer blood bag. The blood bag is made of a modified polyolefin material. The preparation method of the blood stabilizer blood bag comprises the following preparation steps: S1, mixing the raw materials; s2, carrying out melt blending; and S3, film blowing and bag making. Through the synergistic effect of the three-dimensional hydrogen bond network stabilizer and the modified polyolefin material, the preservation period of the red blood cells is prolonged to 45 days from traditional 35 days, a dynamic protection film formed by the stabilizer can inhibit a glycolytic pathway, the 2, 3-DPG content retention rate reaches 85% or above, compared with the prior art, the 2, 3-DPG content retention rate is increased by 15%, the oxygen carrying function of the red blood cells is effectively maintained, and the service life of the red blood cells is prolonged. And after being stored for 45 days, the red blood cell survival rate is still greater than or equal to 92%, the hemolysis rate is less than or equal to 0.05%, and the clinical long-term blood use

The invention discloses an organ maintenance liquid for repairing liver functions as well as a preparation method and application of the organ maintenance liquid. The feed additive comprises the following components: an oxygen-carrying component, succinyl gelatin, sodium chloride, sodium hydroxide, sodium bicarbonate, calcium gluconate, magnesium sulfate, chromium chloride, copper chloride, ferric chloride, manganese chloride, sodium molybdate, sodium selenite, zinc chloride, potassium iodide, sodium fluoride, isoleucine, leucine, lysine acetate, methionine, phenylalanine, threonine, tryptophan, serine, valine and histidine. The injection is prepared from arginine, alanine, asparaginic acid, cysteine, glutamic acid, proline, tyrosine, glycine, sodium hydrogen sulfite, heparin, methylprednisolone sodium succinate, broad-spectrum antibiotics, antifungal drugs, FXR agonists and sterilized water for injection. The oxygen-carrying component is washed red blood cells or concentrated red blood cells or artificial blood. The viscosity of the organ maintenance liquid is closer to that of plasma, the influence on the blood coagulation function and the renal function is avoided, and the organ maintenance liquid has higher safety, can treat ischemia reperfusion injury, repair the liver biliary tract function and remarkably reduce various inflammation indexes, and is used for ischemia-free liver transplantation.

The invention belongs to the technical field of biomedical materials, and particularly relates to a novel organ cryopreservation solution based on PVP (Polyvinyl Pyrrolidone) and containing fluorocarbon emulsion. The invention discloses an organ preserving fluid which takes KPS-1 as a basis, polyvinylpyrrolidone (PVP) as a main anti-freezing component, fluorocarbon emulsion as an oxygen carrying agent and ATP and glucose as energy donors. In addition, the cleaning agent contains the surfactant, trehalose and other components, does not contain DMSO, and is non-toxic. Compared with the traditional UW liquid which can be preserved for 24 hours at 4 DEG C, the oxygen carrying capacity is remarkably improved, ischemia reperfusion injury is reduced, the UW liquid can be preserved for a long time at the ultralow temperature of-80 DEG C, and meanwhile sufficient energy is provided for maintaining metabolism of isolated organs. The cryopreservation time of tissues and organs such as in-vitro liver and kidney can be remarkably prolonged, and the preservation effect is superior to that of UW liquid.

The invention provides an oxygen-carrying material for oxygenation treatment of a water body and a preparation method and application of the oxygen-carrying material. The oxygen-carrying material for oxygenation treatment of the water body, provided by the invention, is a granular porous composite material formed by loading calixarene and / or an inclusion compound thereof on a self-assembled metal organic framework (MOF) material, the porous structure of a self-assembled ZIF-8 matrix material of the porous composite material and polar functional groups (such as hydroxyl, carboxyl, sulfonic acid groups, bridging sulfur atoms and the like) carried by sulfonated thiacalix [4] arene (TCAS) loaded on the surface of the self-assembled ZIF-8 matrix material and / or an inclusion compound of the TCAS and oxygen gas molecules generated in a water body are utilized to interact with each other; efficient loading and controllable release of the oxygen nano bubbles are realized, and sustainable recovery of an anoxic fresh water system is realized; the preparation process is rapid, and large-scale production is easy.

The invention belongs to the technical field of biomedical materials, and particularly relates to injectable-oxygen-carrying-antibacterial composite hydrogel as well as a preparation method and application thereof. The method comprises the following steps: dissolving dopamine hydrochloride in a buffer solution, adding bioactive glass, carrying out ultrasonic treatment to obtain BGs (at) PDA, dispersing the BGs (at) PDA in water, dropwise adding perfluorooctanoyl chloride, and carrying out ultrasonic treatment to obtain fluorinated bioactive glass; the preparation method comprises the following steps: dissolving hyaluronic acid in water, adding sodium periodate, reacting in a dark place, adding ethylene glycol, continuously reacting, dialyzing and freeze-drying to obtain an oxidized hyaluronic acid solid; and dispersing the fluorinated bioactive glass into a gelatin solution, and then uniformly mixing with an oxidized hyaluronic acid solution, thereby obtaining the product. The hydrogel provided by the invention has injectability, excellent mechanical property and oxygen carrying effect, has good antibacterial effect and biocompatibility, can effectively promote bone defect repair, and has wide application prospect in the field of bone tissue engineering.

The application provides a porous hydrogel with high catalytic performance and a preparation method and application thereof, the nanoparticle immobilized enzyme contained in the porous hydrogel enhances the enzyme immobilization effect, plays a certain leakage prevention effect, and prolongs the service life; the metal nanoparticles can perform charge transmission while catalyzing the conversion of glucose, so that the hydrogel has certain conductivity, thereby establishing a good catalytic sensing environment; meanwhile, the porous hydrogel has the catalytic property and oxygen carrying property of the nanoparticle immobilized enzyme and certain viscoelasticity, and has good biocompatibility; the porous hydrogel is formed by heat polymerization, the hydrogel prepared has a reticular structure in the inside, has a high specific surface area, and the preparation method can adjust the pore size of the hydrogel by adjusting the use ratio of the carboxymethylated cellulose nanofiber and betaine, so as to obtain the hydrogel matched with a higher enzyme loading capacity, so that the hydrogel has higher catalytic capacity and has wide application prospects in electrochemical sensors.

The invention provides a device for detecting dynamic oxygen carrying and oxygen release of a membranous hemoglobin solution, an absorbance detection and calculation method, equipment, a medium and a program product, and relates to the technical field of biological detection. A detection bin is arranged in the device, and the device further comprises a sample assembly unit located in the detection bin and used for containing a to-be-detected sample; the sample assembly unit comprises a pore plate bracket, a polytetrafluoroethylene film and cover glass which are sequentially arranged from bottom to top; the polytetrafluoroethylene film tightly covers the upper end of the pore plate bracket, and a to-be-detected sample is added to the upper end of the polytetrafluoroethylene film; and an optical detection unit. The device supports synchronous detection of multi-sample absorbance of a film-shaped hemoglobin solution, carries a polytetrafluoroethylene film, realizes gas exchange based on the characteristics of chemical inertness, ultra-thinness, porosity and the like of the polytetrafluoroethylene film, and simulates a real scene of gas interaction between a hemoglobin spray and the surrounding environment on the surface of a wound surface.

Bio-artificial blood substitutes are provided for intravenous medical use to restore or increase tissue perfusion and oxygen delivery in a pre-hospital or hospital setting. The bio-artificial blood substitutes comprise three modular components (polyethylene glycol (PEG) polymers to increase perfusion, a hemoglobin-based oxygen carrier (HBOC) plus L-arginine to prove oxygen carrying capacity, and a lyophilized fibrinogen or plasma component to provide hemostasis potential, all of which are administered sequentially in modules to treat low perfusion states with possible coagulopathy as seen in blood loss and shock. The three solutions are stable at ambient temperature and are well-suited for use in the field, e.g., when immediate, critical care is needed such as on the battlefield, during natural disasters or other mass casualty events. Other use may include clinical need for transfusion or for peri-surgical resuscitation in patients unable to receive standard blood products.

The application belongs to the technical field of biological medicine and photodynamic therapy, and particularly relates to an aggregation-induced emission photosensitizer with tumor hypoxia relief function and a preparation method and application thereof. The photosensitizer is MeTCP-F, which uses methoxy-modified triphenylamine as an electron donor, benzindole as an electron acceptor, and thiophene as a pi bridge to form a D-pi-A structure, and is connected with a perfluorinated compound through an ester bond; and the MeTCP-F NPs are prepared by using CH3-PEG-DSPE through a nano precipitation method. The photosensitizer has near-infrared fluorescence imaging, active oxygen generation and oxygen carrying capacity, can relieve tumor hypoxia, cooperatively realize the generation of type I and type II active oxygen, and improve the photodynamic therapy effect. The MeTCP-F NPs have good water solubility and high biocompatibility, can be enriched in tumor tissues through the EPR effect, can realize efficient tumor killing under the guidance of near-infrared imaging, have low systemic toxicity, and have application value in the preparation of anti-tumor photodynamic therapy drugs.