88results about How to "Improve the effect of photodynamic therapy" patented technology

The invention provides nano particles compounded of up-conversion nano particles and gold nanorods and a preparation method and application of the nano particles, and belongs to the field of nano biomedicine. The method comprises the following steps: reacting up-conversion nuclear shell nano particles with a photosensitizer; and then, coupling with SiO2-coated gold nanorods to obtain the nano particles compounded of up-conversion nano particles and gold nanorods. The shell thickness of the SiO2-coated gold nanorods is 30-60nm. By means of the surface covalency or photosensitizer absorption of the up-conversion nano particles, the energy transfer efficiency is improved, so that the singlet oxygen yield is improved and the effect of photodynamics therapy is improved. Meanwhile, the acting distance betweeb the gold nanorods and the up-conversion nano particles is regulated to avoid the up-conversion fluorescence quenching phenomenon. According to the invention, upconversion and photodynamics therapy of the photosensitizer are combined with the photo-thermal effect of the SiO2-coated gold nanorods for preparing a synergic treatment nano platform. The purpose of two treatment ways are realized by one excitation light source is reached.

The invention provides a composite gold nanorod carrier having photo-thermal/photodynamic therapy treatment performance and a preparation method thereof. The composite gold nanorod carrier uses a gold nanorod as a core and uses silicon dioxide as a shell layer, a near infrared fluorescent dye for photodynamic therapy is modified on the outer surface of the shell layer. The preparation method comprises the steps that a chloroauric acid solution and a cetyl trimethyl ammonium bromide solution are mixed, a sodium borohydride ice water mixed solution is added to obtain a nano gold seed solution A; a silver nitrate solution and a chloroauric acid solution are added to a binary surface active agent solution, a hydrochloric acid is added after reaction to obtain a solution B, then ascorbic acid and the solution A are added, and a gold nanorod is obtained after reaction; a tetraethoxysilane methanol solution and an aminopropyl trimethoxy silane methanol solution are added to a gold nanorod solution to obtain composite nanoparticles; 1-(3-dimethyl amino propyl)-3-ethyl carbodiimide hydrochloride is added to the near infrared fluorescent dye, then N-hydroxyl succinimide is added to obtain a solution C; the composite nanoparticle solution is mixed with the solution C to obtain a product.

The invention relates to an albumin-stabilized MnO2 nanomaterial as well as a preparation method and an application. The albumin-stabilized MnO2 nanomaterial is a multi-component BMnNSs-HPPH nanocomposite. The nanomaterial is a multi-component nanocomposite (BMnHNCs) with good water dispersity and is formed by loading a photosensitizer HPPH in a BSA-MnO2 nanosheet. By the aid of high reactivity and high specificity of MnO2 for endogenous H2O2 under the acid condition, BMnHNCs can realize NIRF and MRI bimodal imaging response to the tumor microenvironment and tumor microenvironment enhanced photodynamic therapy. The nanocomposite has good biocompability and biodegradability and safe and efficient diagnosis and treatment capacity, will provide a new idea for comprehensively regulating the tumor microenvironment and improving tumor diagnosis and treatment capacity and has greater potentials in the aspects of clinical conversion.

The invention discloses a photosensitizer phospholipid compound, a preparation method of the photosensitizer phospholipid compound, a pharmaceutical composition and application of the photosensitizer phospholipid compound. The pharmaceutical composition is the photosensitizer phospholipid or a combined pharmaceutical composition of the photosensitizer phospholipid and a pharmacologically acceptable carrier, and the pharmaceutical composition is in the form of a liquid preparation, a solid preparation, a semisolid preparation, a capsule, granules, a gel or an injection. The photosensitizer phospholipid compound or a liposome of the photosensitizer phospholipid is applicable to the photodynamic therapy of tumors; the photosensitizer phospholipid compound or the liposome of the photosensitizer phospholipid compound can be rapidly taken by cells and can release a photosensitizer crude drug, and under laser action, the photosensitizer phospholipid compound or the liposome of the photosensitizer phospholipid compound can rapidly release active oxygen to take a strong anti-tumor effect. The photosensitizer phospholipid compound and a liposome nanoparticle thereof can serve as a liquid preparation, a solid preparation, a semisolid preparation, a sterilized preparation and a sterile preparation; and the photosensitizer phospholipid compound and the liposome nanoparticle are low in toxicity and are applicable to the efficient treatment of various tumors.

The invention relates to preparation methods of a natural biomass quantum dot and a biomass quantum dot-copper nanocomposite and application of the natural biomass quantum dot. The preparation methodof the biomass quantum dot comprises the following steps: S1, grinding spinach leaves into paste; S2, adding a mixed solution of ethanol and acetone, uniformly mixing and filtering; S3, adding oleic acid, polyethylene glycol diamine and a magnetic stir bar into a three-neck flask, pumping air out after sealing, introducing argon, stirring, heating to 170 to 190 DEG C, and stopping heating when thesolution is in orange-yellow color; S4, cooling the solution to room temperature, adding a chlorophyll crude extracting solution, heating the solution to 170 to 190 DEG C under the protection of theargon and magnetic stirring, and keeping the temperature for 170 to 190 minutes; S5, naturally cooling the solution to room temperature, and adding concentrated HCl till the solution is in strong acidity; S6, standing for layering, and collecting subnatant; S7, adjusting the pH value of the solution to neutral with a saturated NaOH solution, filtering larger particles, and performing dialysis to obtain a biomass quantum dot solution. The biomass quantum dot solution has the characteristics of high fluorescence intensity, high light stability and high biocompatibility.

The invention discloses a multifunctional contrast agent of oxygen carrying liquid fluorocarbon. The multifunctional contrast agent is of a spherical shell-core structure; the core is prepared from liquid fluorocarbon and indocyanine green. The invention aims at solving the technical problems to provide the multifunctional contrast agent of oxygen carrying liquid fluorocarbon and the preparation method. The multifunctional contrast agent has high biological safety and stability, and is used for the multi-mode nanometer contrast agent guided breast cancer light treatment.

The invention belongs to the field of nanometer materials, and relates to a porphyrin metal-organic framework (PMOF) with a hollow nanometer structure as well as a preparation method and application thereof. The material has a huge cavity and a casing, wherein the casing is made of the metalloporphyrin framework material. The size of the nanometer material is 20 to 200 nm; the casing layer thickness is 5 to 50 nm; the cavity diameter is 10 to 200 nm. The huge cavity of the hollow PMOF nano particles is used for loading perfluorocarbon; the perfluorocarbon has high oxygen dissolution performance; oxygen can be efficiently loaded; more oxygen is provided for photodynamics therapy, so that more singlet oxygen is generated; the photodynamic curative effect is enhanced. The huge cavity of the hollow PMOF nano particles and the casing layer pore can effectively load diagnostic reagents including chemotherapeutic drugs, optothermal reagents, imaging molecules and the like. The hollow PMOF haswide application prospects when being used as a novel nanometer drug carrier system.

The invention discloses a preparation method of a rare earth upconversion nanoparticle/bismuth vanadate nano composite material with an antitumor effect, and relates to a synthesis method of an antitumor nano material. The invention aims to solve the problems that BiVO4 synthesized by the existing method is large in size and the application of BiVO4 in the field of biomedicine is limited. The preparation method comprises the following steps: 1, preparing synthetic core structure nanoparticles; 2, synthesizing core-shell structure nanoparticles; 3, preparing hydrophilic up-conversion nanoparticles; 4, synthesizing zinc nitrophthalocyanine; 5, synthesizing amino zinc phthalocyanine; 6, synthesizing a BiVO4 nanosheet by a high-temperature pyrolysis method; 7, connecting UCNPs with zinc aminophthalocyanine; and 8, preparing and modifying the composite material PVP/UCNPs-ZnPc@BiVO4. The preparation method is used for preparing the rare earth upconversion nanoparticle/bismuth vanadate nano composite material with the antitumor effect.

The invention provides a platinum@polydopamine-dihydroporphin nano composite material. The surface of platinum is coated with polydopamine to form a core-shell structure, and dihydroporphin is loadedas a photosensitizer. The composite material has low toxicity, significant biocompatibility and good biodegradability, and can be used as a photothermal therapy reagent and a photodynamic therapy reagent. Besides, Pt can assist in the photodynamic therapy process, relieve the problem of oxygen deficit of a tumor part and finally achieve the PTT-PDT synergistic treatment effect. The invention alsodiscloses a preparation method and application of the composite material.

The invention provides nanocomposite particles and a preparation method thereof. The preparation method of the nanocomposite particles comprises the following steps: dissolving ZrCl4 in N,N-dimethylmethylamide so as to form a ZrCl4 solution; sequentially adding terephthalic acid, benzoic acid, hydrochloric acid and meso-tetra(4-carboxyphenyl)porphine into the ZrCl4 solution so as to obtain organometallic framework nanoparticles embedded with porphyrin molecules; adding the organometallic framework nanoparticles into a buffer solution; and then, adding dopamine so as to form polydopamine by polymerization of dopamine molecules, thereby wrapping outer surface of the organometallic framework nanoparticles with the polydopamine so as to form nanocomposite particles with a core-shell structure,wherein thickness of the polydopamine layers is 5-50nm and size of the nanocomposite particles is 60-500nm. The nanocomposite particles prepared by the preparation method are uniform in appearance, good in dispersivity, low in biological toxicity, and excellent in photothermal effects and photodynamic therapeutic effects; and thus, the nanocomposite particles have good curative effects on tumors.

The invention discloses black phosphorus quantum dot/platinum hybrid mesoporous silica nanoparticles and a preparation method and application thereof. The black phosphorus quantum dots/platinum hybridnanoparticles loaded with platinum nanoparticle in mesopores are synthesized through a one-step hydrothermal method and an in-situ growth method, so that the solution stability of black phosphorus quantum dots can be significantly improved; and hydrogen peroxide in tumor microenvironment undergoes catalytic reaction with the platinum nanoparticles to form oxygen, so that the photodynamic therapyeffect, of the black phosphorus quantum dots, on tumors is further enhanced, and then a good biomedical application prospect is provided.

The invention relates to asymmetric dendritic metalloporphyrin as well as a preparation method and an application thereof and belongs to the technical field of photodynamic treatment. The asymmetric dendritic metalloporphyrin is firstly prepared from 5-p-hydroxyl phenyl-10,15,20-trichlorophenylporphyrin through coordination reaction and substitution reaction. Compared with symmetric dendritic metalloporphyrin, the asymmetric dendritic metalloporphyrin has a larger decorative space; the introduction of functional groups including halogen and the like enables porphyrin to have higher three-line-state quantum yield and single-line-state oxygen quantum yield so as to greatly improve the photodynamic treatment effect; and meanwhile, the asymmetric dendritic metalloporphyrin molecule has very strong light cytotoxicity and is a promising photodynamic treatment medicine. The structure of the asymmetric dendritic metalloporphyrin is shown as the specification, wherein R is a dendritic polyphenyl ether substituent.

The invention discloses a rare earth upconversion diagnosis-and-treatment-integrated nanocomposite material. The rare earth upconversion diagnosis-and-treatment-integrated nanocomposite material is acuprous oxide modified rare earth upconversion nanocomposite prepared by the following steps: using oil-soluble upconversion nanoparticles (UCNPs) as cores; and then, allowing epitaxial growth of cuprous oxide (Cu(2)O) on surfaces of the UCNPs so as to form shells, thereby obtaining the cuprous oxide modified rare earth upconversion nanocomposite grown by interface nucleation. The Cu(2)O can transfer fluorescence resonance energy with upconversion emission of the UCNPs excited by near-infrared light so as to produce sufficient active oxygen, thereby meeting needs of photodynamic therapy; and moreover, the Cu(2)O can synchronously cooperate with the oil-soluble UCNPs so as to realize integrated diagnosis and treatment. The invention further provides a preparation method of the rare earth upconversion diagnosis-and-treatment-integrated nanocomposite material. The preparation method of the rare earth upconversion diagnosis-and-treatment-integrated nanocomposite material comprises the following steps: using the oil-soluble UCNPs as cores; and then, allowing interface nucleation growth of the (Cu(2)O) on the surfaces of the UCNPs. The rare earth upconversion diagnosis-and-treatment-integrated nanocomposite material prepared by the preparation method is uniform in size, good in stability, and excellent in biocompatibility; and thus, the nanocomposite material can be used in imaging diagnosis guided photodynamic therapy so as to meet needs of clinical diagnosis and treatment integration.

The invention discloses nano emulsion as well as a preparation method and application thereof. The nano emulsion comprises a Bodipy amphiphilic compound and perfluocarbon loaded inside the Bodipy amphiphilic compound, wherein the nano emulsion carries oxygen gas. The nano emulsion can realize activated fluorescent, opto-acoustic and 19F magnetic resonance imaging for a tumor site as well as photodynamic therapy of tumors. The preparation process is simple, is convenient to operate, does not need complex and dear equipment, easily realizes industrial production, and has a good application prospect in the field of tumor diagnosis and treatment.

The invention discloses a photodynamic therapy agent for relieving tumor hypoxia as well as a preparation method and application thereof, and belongs to the technical field of medicines. The photodynamic therapy agent is a metal-organic framework nano composite material loaded with O2. The metal-organic framework nano composite material is metal-organic framework nano particles Zr-TCPP (Fe), the interiors of nano frameworks of the nano particles Zr-TCPP (Fe) are modified with perfluorooctanoic acid, and meanwhile glucose oxidase GOx is further adsorbed in pore channels of the nano particles Zr-TCPP (Fe). According to the invention, a drug delivery system capable of catalyzing tumor to endogenously generate O2 is designed while O2 is delivered to tumor tissue, so that the hypoxic environment can be remarkably improved, and the photodynamic therapy effect is enhanced. In addition, the synthetic steps of the therapy agent are relatively simple, and the yield is relatively high; and a synthetic method is simple and relatively low in cost, so that the method is suitable for large-scale production.

The invention discloses a preparation method and application of a novel phototherapy nano preparation based on polyhedral oligomeric silsesquioxane (POSS). The phototherapy nano preparation is prepared by forming nano particles by chemical cross-linking reaction between amino of the POSS and carboxyl of a photosensitizer and then performing surface pegylation. The nano preparation has the benefits that the photosensitizer is taken as one part by utilizing a chemical cross-linking method, so that the ultra-high drug loading capacity is realized; the advance drug leakage is avoided; a self-quenching effect of the photosensitizer is inhibited. Meanwhile, the drug has good water dispersibility, stability and biosafety. In addition, the phototherapy nano preparation can be endocytosed by cancer cells in large quantities and efficiently kill the cancer cells under light conditions. An in-vivo animal experiment proves that the phototherapy nano preparation can be enriched to a tumor area through passive targeting, so that tumor imaging and photodynamic therapy are realized.

The invention relates to the technical field of synthesizing covalent organic framework materials with photodynamic therapeutic effects, and in particular relates to a covalent organic framework material with photodynamic therapeutic effects and a preparation method thereof. The molecular structural formula of the covalent organic framework material with photodynamic therapeutic effects is as shown in the specification, the covalent organic framework material with photodynamic therapeutic effects has high crystalline state, specific surface area, small biological toxicity and a particle size of about 100 nm, and is very suitable for biological experiments; according to the invention, after monomers which do not have the capability of generating active oxygen are assembled into corresponding two-dimensional COF samples, the capability of generating the active oxygen is obviously enhanced, and the capability of generating the active oxygen of the covalent organic framework material is better than that of the classical porphyrin metal organic framework (MOF) and PCN 224, and the covalent organic framework material has excellent photodynamic therapy effect.

The embodiment of the invention discloses a fullerene phthalocyanine derivative as well as a preparation method and application thereof. The fullerene phthalocyanine derivative has the advantages of novel structure, small possibility of generating photobleaching, light degradation and aggregation, high phototoxicity and low dark toxicity and is more stable under an illumination condition.

Compositions of the invention include glycoproteins, such as transferrin, and metal-based coordination complexes, which are preferably chemotherapeutic compounds and more preferably tunable photodynamic compounds. The compositions are useful as in vivo diagnostic agents, and as therapeutic agents for treating or preventing diseases including those that involve hyperproliferating cells in their etiology, such as cancer. Compositions of the invention are further capable of destroying microbial cells, such as bacteria, fungi, and protozoa, and destroying viruses. Treatment methods of the invention can treat conditions throughout the body, including conditions located across the blood-brain barrier, the retina-blood barrier and the blood-cerebrospinal fluid barrier.

The invention discloses a quinoxaline-based D-A-pi-A type organic photosensitizer as well as a synthesis method and application thereof, and belongs to the field of tumor photodynamic therapy. According to the invention, a series of novel D-A-pi-A structure near-infrared organic photosensitizers are synthesized by taking triphenylamine as an electron donor, diphenyl substituted quinoxaline as an additional receptor and pyridinium as a terminal electron acceptor. The introduction of diphenyl-substituted quinoxaline can effectively red-shift the light absorption and emission spectrum of the photosensitizer, and on the other hand, the existence of benzene rings on triphenylamine and quinoxaline with propeller structures can effectively inhibit the pi-pi accumulation effect between molecules,thereby improving the photodynamic therapy effect of the photosensitizer. By integrating the effects of the two aspects, the organic photosensitizer has a good application prospect in tumor photodynamic therapy.