The invention discloses a suspension
cell sphere based on self-floating
microbubbles as well as a preparation method and application of the suspension
cell sphere, and belongs to the technical field of three-dimensional culture of cells. Aiming at the problem of complicated operation caused by dependence on an
external field or exogenous matrix in the existing three-dimensional culture technology, the method comprises the following steps: mixing and adsorbing cells and
polylysine modified self-floating
microbubbles in an adsorption solution, and then inoculating the cells and the
polylysine modified self-floating
microbubbles to a
polydimethylsiloxane hydrophobic pore plate in a
liquid drop form; enabling the
cell-microbubble complex to float to the top end of the
liquid drop to be enriched by utilizing the
buoyancy of the self-floating microbubble; transferring the complex to fresh culture liquid drops through a
liquid drop sandwich method to realize mild separation of unbound cells; and finally, culturing to form the suspension cell sphere. The method is suitable for preparing multicellular tumor balls and culture kits, and can be used in the fields of
drug screening, tumor biological research, tissue regeneration and the like.