8 results about "Epididymis" patented technology

This invention discloses a Fam205a1 Methods for constructing and applying gene knockout mouse models. The construction method utilizes CRISPR / Cas9 gene editing technology, targeting mice. Fam205a1 Gene-designed specific sgRNAs for knockout Fam205a1 The regions of exons 1 through 4 of the gene are obtained. Fam205a1 Gene knockout mice. Male mice in this model exhibit infertility, specifically characterized by impaired spermatogenesis, reduced sperm motility, abnormal sperm morphology (particularly head and acrosome defects), and spermatogenic cell apoptosis. However, the weight and appearance of their testes and epididymis show no significant difference from the wild type. The mouse model constructed in this invention has stable genetic traits and a well-defined phenotype, making it an ideal animal model for studying the mechanisms of idiopathic male infertility, screening drugs for treating male infertility, or developing male contraceptives.

The present application relates to the technical field of extracellular vesicle sorting, and particularly relates to a epididymal body specific sorting marker, and an epididymal body sorting kit and method. The present application finds that CD52 can be used as a specific marker for sorting epididymal bodies. The present application is the first time to directly separate epididymal bodies from human seminal plasma, the sample is easy to obtain, and the operation process is non-invasive, safe, and the separation path is simple and easy to operate. In addition, it provides a method for the separation of other tissue-specific EV subpopulations. The present application can contribute to further explore the effect of epididymal bodies on sperm function and improve sperm function, and epididymal bodies as a marker have great potential for clinical diagnosis and treatment.

The invention discloses a porcine epididymal cell-derived extracellular vesicle as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. By optimizing the preparation method of the extracellular vesicles, the extracellular vesicles with high purity and standard shape are successfully obtained. Furthermore, by virtue of a proteomics technology, the composition characteristics of rich key functional proteins related to oxidation resistance, movement regulation and fertilization are systematically clarified for the first time. Functional experiments prove that the vesicle can be effectively attached to sperms, the sperm motility is remarkably improved under the low-temperature preservation condition, the oxidation resistance is enhanced, membrane damage, mitochondrial dysfunction and DNA damage are relieved, and the specific protection effect on the sperms is shown. The invention provides a reliable material basis and theoretical basis for developing a novel sperm preservative and an assisted reproduction technology based on extracellular vesicles.

The application discloses a vas deferens contraceptive device and a preparation method thereof, and relates to the technical field of medical devices. The device comprises a tubular outer wall, the inside of the tubular outer wall is filled with a sperm blocking layer and a mechanical support layer, the sperm blocking structure is a pore structure that can block sperm, and the mechanical support structure is a three-period minimal surface structure. The combination of the two structures can prevent sperm from passing through the vas deferens while ensuring the normal passage of tissue fluid. The device is mainly prepared through three steps of model slice design, ink configuration, 3D printing and device post-processing. Compared with vasectomy, the vas deferens contraceptive device and the preparation method thereof can avoid complications and fertility damage caused by epididymal stasis, and the implantation of the device has a smaller incision than vasectomy and is convenient for processing and manufacturing.

The invention discloses a method for establishing an oligoasthenospermia animal model by utilizing flunarizine and application of the method, and belongs to the technical field of biological medicines. The method comprises the following steps: randomly grouping SPF-level experimental animals; injecting a flunarizine FNZ solution into the abdominal cavity continuously for 7 days; and verifying the oligospermia and asthenozoospermia indexes. In the animal model constructed by the method, the number and activity of sperms in the epididymis of an experimental animal are reduced, the mitochondrial membrane potential of the sperms is reduced due to FNZ, and ATP production is reduced, which indicates that the FNZ reduces the quality of the sperms. Therefore, the method for constructing the oligoasthenospermia model provides a proper animal model for researching male infertility diseases such as oligoasthenospermia and the like; the animal model can be used for animal experiments and new drug research and development for researching and developing occurrence and development mechanisms of male infertility diseases.

This invention discloses a method related to the size of the testes and epididymis of Hu sheep. DRIVE Gene molecular markers and their applications, wherein the molecular markers are composed of DRIVE The gene sequence was obtained by amplification using designed specific primers, and its nucleotide sequence is shown in SEQ ID NO.1. There is an A / C base mutation at position 147 bp in SEQ ID NO.1, which leads to Mlu I-RFLP restriction polymorphism. Analysis of Hu sheep... DRIVE The correlation between genotyping and testicular and epididymal size revealed that Hu sheep individuals had three genotypes: AA, AC, and CC. Individuals with the AC genotype showed significantly higher testicular and total epididymal weights than those with the AA and CC genotypes. Using the molecular markers of this invention for Hu sheep breeding offers high accuracy and allows for the selection of high-fertility Hu sheep rams in their early years, enabling early selection and accelerating the breeding process.

The invention discloses application of eurycoma longifolia in reduction of toxic and side effects of tripterygium wilfordii, according to tests, it is found that eurycoma longifolia can significantly improve testis indexes induced by tripterygium wilfordii polyglycosides, epididymis indexes are reduced, and testis pathological injuries induced by tripterygium wilfordii polyglycosides are relieved. Fluorescent quantitative PCR (Polymerase Chain Reaction) analysis shows that eurycoma longifolia can obviously induce expression up-regulation of reproductive genes Tnp1, Atp1a4, Spaata6 and the like, and the toxicity of testis induced by tripterygium glycosides is reduced. The invention provides a new idea for reducing the toxic and side effects of tripterygium wilfordii.

The invention discloses an intestinal bacterium for improving semen quality and application thereof, and belongs to the field of microbial technology and biological medicine. In order to solve the problem that in the prior art, specific probiotics are not used for improving male reproductive injury induced by high fat diet, the invention provides a method for applying the allobacter faecalis strain (A.s) or an inactivated form (P-A.s) of the allobacter faecalis strain (A.s) to the male reproductive injury. Animal experiments show that compared with a high fat diet model group, supplementation of A.s or P-A.s can significantly inhibit abnormal weight growth, improve testis index, improve sperm density, vitality, movement speed and testicular testosterone level, and can effectively repair tissue form damage of testis and epididymis and abnormal sperm ultrastructure. Wherein the improvement effect of the inactivated bacterium form P-A.s is particularly prominent.