4 results about "Epididymis" patented technology

This invention discloses a Fam205a1 Methods for constructing and applying gene knockout mouse models. The construction method utilizes CRISPR / Cas9 gene editing technology, targeting mice. Fam205a1 Gene-designed specific sgRNAs for knockout Fam205a1 The regions of exons 1 through 4 of the gene are obtained. Fam205a1 Gene knockout mice. Male mice in this model exhibit infertility, specifically characterized by impaired spermatogenesis, reduced sperm motility, abnormal sperm morphology (particularly head and acrosome defects), and spermatogenic cell apoptosis. However, the weight and appearance of their testes and epididymis show no significant difference from the wild type. The mouse model constructed in this invention has stable genetic traits and a well-defined phenotype, making it an ideal animal model for studying the mechanisms of idiopathic male infertility, screening drugs for treating male infertility, or developing male contraceptives.

The present application relates to the technical field of extracellular vesicle sorting, and particularly relates to a epididymal body specific sorting marker, and an epididymal body sorting kit and method. The present application finds that CD52 can be used as a specific marker for sorting epididymal bodies. The present application is the first time to directly separate epididymal bodies from human seminal plasma, the sample is easy to obtain, and the operation process is non-invasive, safe, and the separation path is simple and easy to operate. In addition, it provides a method for the separation of other tissue-specific EV subpopulations. The present application can contribute to further explore the effect of epididymal bodies on sperm function and improve sperm function, and epididymal bodies as a marker have great potential for clinical diagnosis and treatment.

The invention discloses a porcine epididymal cell-derived extracellular vesicle as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. By optimizing the preparation method of the extracellular vesicles, the extracellular vesicles with high purity and standard shape are successfully obtained. Furthermore, by virtue of a proteomics technology, the composition characteristics of rich key functional proteins related to oxidation resistance, movement regulation and fertilization are systematically clarified for the first time. Functional experiments prove that the vesicle can be effectively attached to sperms, the sperm motility is remarkably improved under the low-temperature preservation condition, the oxidation resistance is enhanced, membrane damage, mitochondrial dysfunction and DNA damage are relieved, and the specific protection effect on the sperms is shown. The invention provides a reliable material basis and theoretical basis for developing a novel sperm preservative and an assisted reproduction technology based on extracellular vesicles.

The invention discloses an intestinal bacterium for improving semen quality and application thereof, and belongs to the field of microbial technology and biological medicine. In order to solve the problem that in the prior art, specific probiotics are not used for improving male reproductive injury induced by high fat diet, the invention provides a method for applying the allobacter faecalis strain (A.s) or an inactivated form (P-A.s) of the allobacter faecalis strain (A.s) to the male reproductive injury. Animal experiments show that compared with a high fat diet model group, supplementation of A.s or P-A.s can significantly inhibit abnormal weight growth, improve testis index, improve sperm density, vitality, movement speed and testicular testosterone level, and can effectively repair tissue form damage of testis and epididymis and abnormal sperm ultrastructure. Wherein the improvement effect of the inactivated bacterium form P-A.s is particularly prominent.