13 results about "Epididymis" patented technology

This invention discloses a Fam205a1 Methods for constructing and applying gene knockout mouse models. The construction method utilizes CRISPR / Cas9 gene editing technology, targeting mice. Fam205a1 Gene-designed specific sgRNAs for knockout Fam205a1 The regions of exons 1 through 4 of the gene are obtained. Fam205a1 Gene knockout mice. Male mice in this model exhibit infertility, specifically characterized by impaired spermatogenesis, reduced sperm motility, abnormal sperm morphology (particularly head and acrosome defects), and spermatogenic cell apoptosis. However, the weight and appearance of their testes and epididymis show no significant difference from the wild type. The mouse model constructed in this invention has stable genetic traits and a well-defined phenotype, making it an ideal animal model for studying the mechanisms of idiopathic male infertility, screening drugs for treating male infertility, or developing male contraceptives.

The invention discloses application of portulaca oleracea polysaccharide in preparation of a feed additive for preventing male animal reproductive injury caused by zearalenone. The animal is a lactation animal. Experimental verification proves that the portulaca oleracea polysaccharide has the following effects: (1) the portulaca oleracea polysaccharide can effectively relieve the damage of ZEA to a male reproductive system and recover the normal physiological status and sperm quantity of reproductive organs of male animals; (2) the damage of ZEA to male testis epididymis tissues can be obviously reduced; and testis and epididymis organ coefficient increase and tissue structure swelling caused by the testis and epididymis are improved, and an excellent protection effect is achieved. (3) serum androgen secretion disorder caused by ZEA can be effectively recovered; normal physiological functions are recovered by regulating secretion of testosterone and dihydrotestosterone. Therefore, when male reproductive injury caused by zearalenone of male animals is prevented, the portulaca oleracea polysaccharide can be added into animal feed as a feed additive.

The present application relates to the technical field of extracellular vesicle sorting, and particularly relates to a epididymal body specific sorting marker, and an epididymal body sorting kit and method. The present application finds that CD52 can be used as a specific marker for sorting epididymal bodies. The present application is the first time to directly separate epididymal bodies from human seminal plasma, the sample is easy to obtain, and the operation process is non-invasive, safe, and the separation path is simple and easy to operate. In addition, it provides a method for the separation of other tissue-specific EV subpopulations. The present application can contribute to further explore the effect of epididymal bodies on sperm function and improve sperm function, and epididymal bodies as a marker have great potential for clinical diagnosis and treatment.

The invention discloses a porcine epididymal cell-derived extracellular vesicle as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. By optimizing the preparation method of the extracellular vesicles, the extracellular vesicles with high purity and standard shape are successfully obtained. Furthermore, by virtue of a proteomics technology, the composition characteristics of rich key functional proteins related to oxidation resistance, movement regulation and fertilization are systematically clarified for the first time. Functional experiments prove that the vesicle can be effectively attached to sperms, the sperm motility is remarkably improved under the low-temperature preservation condition, the oxidation resistance is enhanced, membrane damage, mitochondrial dysfunction and DNA damage are relieved, and the specific protection effect on the sperms is shown. The invention provides a reliable material basis and theoretical basis for developing a novel sperm preservative and an assisted reproduction technology based on extracellular vesicles.

The application discloses a vas deferens contraceptive device and a preparation method thereof, and relates to the technical field of medical devices. The device comprises a tubular outer wall, the inside of the tubular outer wall is filled with a sperm blocking layer and a mechanical support layer, the sperm blocking structure is a pore structure that can block sperm, and the mechanical support structure is a three-period minimal surface structure. The combination of the two structures can prevent sperm from passing through the vas deferens while ensuring the normal passage of tissue fluid. The device is mainly prepared through three steps of model slice design, ink configuration, 3D printing and device post-processing. Compared with vasectomy, the vas deferens contraceptive device and the preparation method thereof can avoid complications and fertility damage caused by epididymal stasis, and the implantation of the device has a smaller incision than vasectomy and is convenient for processing and manufacturing.

The invention discloses a method for establishing an oligoasthenospermia animal model by utilizing flunarizine and application of the method, and belongs to the technical field of biological medicines. The method comprises the following steps: randomly grouping SPF-level experimental animals; injecting a flunarizine FNZ solution into the abdominal cavity continuously for 7 days; and verifying the oligospermia and asthenozoospermia indexes. In the animal model constructed by the method, the number and activity of sperms in the epididymis of an experimental animal are reduced, the mitochondrial membrane potential of the sperms is reduced due to FNZ, and ATP production is reduced, which indicates that the FNZ reduces the quality of the sperms. Therefore, the method for constructing the oligoasthenospermia model provides a proper animal model for researching male infertility diseases such as oligoasthenospermia and the like; the animal model can be used for animal experiments and new drug research and development for researching and developing occurrence and development mechanisms of male infertility diseases.

The invention relates to the technical field of sperm collection, and discloses a sperm collection method for a reproductive medicine laboratory, which comprises the following steps: cleaning and operating an experimental mouse to accurately collect sperms of the experimental mouse; the method comprises the following steps: firstly, cleaning the abdomen of an experimental mouse and performing surgical incision to expose testis and epididymis; then, separating the epididymis from the testis by using a sterilized appliance, and flushing the epididymis with normal saline by using an injector; sperms are extracted from the flushing fluid, and distribution of the sperms in liquid drops is obtained through computer simulation and image analysis; according to the method, accurate image acquisition and simulation calculation are combined, sperm distribution can be accurately judged, the suction strategy is optimized, and it is ensured that the maximum number of sperms are collected; finally, the sperms are separated through centrifugal operation; the method provides a new accurate scheme for sperm collection, and has high reliability and operability.

This invention discloses a method related to the size of the testes and epididymis of Hu sheep. DRIVE Gene molecular markers and their applications, wherein the molecular markers are composed of DRIVE The gene sequence was obtained by amplification using designed specific primers, and its nucleotide sequence is shown in SEQ ID NO.1. There is an A / C base mutation at position 147 bp in SEQ ID NO.1, which leads to Mlu I-RFLP restriction polymorphism. Analysis of Hu sheep... DRIVE The correlation between genotyping and testicular and epididymal size revealed that Hu sheep individuals had three genotypes: AA, AC, and CC. Individuals with the AC genotype showed significantly higher testicular and total epididymal weights than those with the AA and CC genotypes. Using the molecular markers of this invention for Hu sheep breeding offers high accuracy and allows for the selection of high-fertility Hu sheep rams in their early years, enabling early selection and accelerating the breeding process.

The invention discloses application of eurycoma longifolia in reduction of toxic and side effects of tripterygium wilfordii, according to tests, it is found that eurycoma longifolia can significantly improve testis indexes induced by tripterygium wilfordii polyglycosides, epididymis indexes are reduced, and testis pathological injuries induced by tripterygium wilfordii polyglycosides are relieved. Fluorescent quantitative PCR (Polymerase Chain Reaction) analysis shows that eurycoma longifolia can obviously induce expression up-regulation of reproductive genes Tnp1, Atp1a4, Spaata6 and the like, and the toxicity of testis induced by tripterygium glycosides is reduced. The invention provides a new idea for reducing the toxic and side effects of tripterygium wilfordii.

The invention discloses a construction method of a Pnldc1 gene-deleted spermatogenesis disorder mouse model, the Pnldc1 gene has a relationship with spermatogenesis disorder or fertility dysfunction, the volume of the testis of a Pnldc1 gene-deleted male mouse is significantly reduced compared with that of a wild mouse, the arrangement of seminiferous tubules in the testis of the male mouse is disordered, the number of long sperm cells is reduced, and the seminiferous tubules in the testis of the male mouse are reduced. In the prior art, no sperm exists in epididymis, the head of a mouse sperm is abnormal in development, 9 + 2 microtubules are deleted, and any offspring cannot be obtained after the mouse is mated with a female mouse, gene editing is carried out in the early development stage of a fertilized egg, the obtained positive mouse is high in chimeric rate, and the passage probability is larger than that of a blastocyst injection ES cell. In addition, the invention can provide a better model for the research of male sterility. Therefore, the male mouse with Pnldc1 gene deletion has the advantages of stability and thorough spermatogenic function inhibition.

This invention provides an electrochemical aptasensor for detecting human epididymis protein 4 (HE4) in ovarian cancer. Using PDDA@Ni3(HITP)2 / AuNPs as the sensing interface and NH2-MIL-53(Al)@KB / AuNPs / Tb / SP as the tracer marker, the aptasensor's electrical signal is synergistically amplified, enabling ultrasensitive detection of HE4. This method provides a new diagnostic approach for early diagnosis of ovarian cancer. Compared with traditional HE4 detection methods, the present method offers advantages such as high sensitivity, strong specificity, rapid detection, ease of operation, low-cost equipment and materials, and no pollution.

The invention discloses application of polydatin in preparation of a medicine for improving and repairing cryptorchis spermatogenic function damage, and belongs to the technical field of biological medicine. The medicine takes polydatin as a main active ingredient. Experiments prove that the polydatin can effectively improve the spermatogenic function, promote the reproductive repair of a mouse with cryptorchis and improve the hormone release level of serum testosterone and gonadotropin, the hormone is beneficial to spermatogenesis, the repair effect has a dose effect, and the repair effect is optimal when the polydatin concentration is 50-100mg / kg. In addition, metabolome in the testis of the mouse can be adjusted, oxidative stress and inflammatory response are relieved, and therefore sperm counting and epididymis weight are affected and improved. According to the scheme, an experimental basis is provided for application of polydatin in the field of reproductive health, and a new candidate medicine is also provided for treatment of male infertility such as hidden testis.

The invention discloses an intestinal bacterium for improving semen quality and application thereof, and belongs to the field of microbial technology and biological medicine. In order to solve the problem that in the prior art, specific probiotics are not used for improving male reproductive injury induced by high fat diet, the invention provides a method for applying the allobacter faecalis strain (A.s) or an inactivated form (P-A.s) of the allobacter faecalis strain (A.s) to the male reproductive injury. Animal experiments show that compared with a high fat diet model group, supplementation of A.s or P-A.s can significantly inhibit abnormal weight growth, improve testis index, improve sperm density, vitality, movement speed and testicular testosterone level, and can effectively repair tissue form damage of testis and epididymis and abnormal sperm ultrastructure. Wherein the improvement effect of the inactivated bacterium form P-A.s is particularly prominent.