137 results about "Epididymis" patented technology

A method and device to conform with the glans penis to recover ejaculated semen completely, to prevent the loss of initial sperm rich epididymal fractions, to avoid the use of a condom for masturbation, to eliminate the multi-step transfers of semen following ejaculation that are common with current methods, and to provide a single device that contains a semen collecting chamber that fits onto a glans penis. The device provides a storage and measuring reservoir, an optional cap, and a vertical stand in one integrally formed module in a sterile pack. The device and method will have multiple uses in a variety of contexts including, but not limited thereto: in the diagnosis of infertility, in semen donation, in vitro fertilization (IVF)/intra-cytoplasmic sperm injection (ICSI) clinics, hospitals, forensic laboratories and research laboratories and will be included in kits intended for over-the-counter sperm testing devices

The invention belongs to the field of application of probiotics, and particularly relates to application of an Akkermansia muciniphila BAA-835 bacterial strain on preparation medicines for treating or preventing obesity. C57/BL6 male SPF-level mice in a nest are randomly divided into a comparison group and a bacteria intragastric administration group, according to a check experiment, an Akkermansia muciniphila BAA-835 bacterial strain suspension intragastric administration experiment is carried out on the mice of the bacteria intragastric administration group once every day, and lasts for 5 weeks, through the experiment, weight and body fat of the mice in the bacteria intragastric administration group are reduced obviously, and by the Akkermansia muciniphila BAA-835 bacterial strain, content of subcutaneous fat of groins of the mice and content of fat beside epididymides of the mice are reduced quite effectively, and therefore, the bacterial strain can be used for treating or preventing the obesity.

The invention relates to an analysis and detection technology of a human ovarian cancer tumor marker (human epididymis protein 4, HE4), in particular to a time-resolved fluorescence (TRF) immunized detection method and a kit of HE4, which is used for clinical auxiliary diagnosis, curative effect observation and prognosis judgment of the ovarian cancer. The invention comprises the following contents: constructing HE4 recombinant plasmids; expressing and purifying HE4 protein; immunizing BALB/c mice by the purified HE4 protein to prepare monoclonal antibodies; matching the obtained monoclonal antibodies to obtain two hybrid tumor cell strains (5A3 and 6C2) for secreting monoclonal antibodies of different epitopes of HE4 antigens, and marking the 5A3 monoclonal antibodies by the rare earth element Eu3+; and taking the unmarked 6C2 monoclonal antibodies as capture antibodies for coating a solid phase carrier, and taking the 5A3 monoclonal antibodies marked with the Eu3+ as detection antibodies to establish a double-antibody sandwich method for detecting HE4, thereby realizing the TRF immunized analysis of the invention.

The invention relates to a preparation method of a diagnostic reagent kit for early diagnosis and recurrence monitoring, and the like of ovarian cancer. The preparation method comprises the following steps of: the recombination expression of a marker HE4 (Human Epididymis Protein) in eukaryotic cells, monoclonal and polyclonal antibody preparation by HE4 obtained by the recombination expression in the eukaryotic cells, and the diagnostic reagent kit (a double antibody sandwich method and an enzyme-linked immunization method) development by the prepared monoclonal and polyclonal antibodies. The invention can ensure that HE4 obtained by the recombination expression in the eukaryotic cells by utilizing a gene engineering method has the same glycosylation degree with natural HE4; the space conformation thereof more approaches to a natural antigen; the prepared monoclonal and polyclonal antibody is used as a coating antibody, and the other monoclonal antibody is used as an enzyme mark antibody to develop the reagent kit; and the sensitivity and the specificity are higher.

The invention provides a quantitative determination kit for human epididymis secretory protein 4 (HE4), which is applicable to a full-automatic chemiluminescence immunoassay analyzer, in particular relates to a preparation process of a kit for quantitatively detecting the concentration level of HE4 in human serum by adopting a dual-antibody sandwich acridinium ester-marked tubular chemiluminescence method. The kit adopts the dual-antibody sandwich immunoassay and utilizes a chemiluminescence magnetic microsphere immune technology, magnetic microspheres which are coated with anti-HE4 antibodies are specifically combined with the HE4 in a standard product/sample in a reaction cup and then react with another anti-HE4 antibody marked by the acridinium ester to form an immune complex, and the immune complex has the acid-alkali chemical reaction in a pre-trigger solution and a trigger solution, so that relative light unit (RLU/s) of the chemiluminescence reaction can be measured; the content of the HE4 in the standard product/sample is in direct proportion to the relative light unit (RLU/s) measured by an optical system, and the content of HE4 in a serum sample can be quantitatively measured by virtue of standard curve fitting. The quantitative determination kit has advantages of high sensitivity, high specificity, good stability, simplicity and convenience in operation, low cost and the like.

The provided human epididymis specific express protein ESC42 with coded sequence can be prepared by chemical method or genetic engineering technique, and can be used to prepare antibacterial drug or new contraceptive.

The invention relates to a stasis-removing and inflammation-diminishing traditional Chinese medicine composition, stasis-removing and inflammation-diminishing pills and a preparation method thereof. The traditional Chinese medicine composition comprises 25 traditional Chinese medicines of rhizome pleionis, rhizoma anemones raddeanae, rhizoma bolbostemmae, fennel, radix aucklandiae, vinegar-baked bupleurum root, rhizoma curculiginis, lychee seed, trifoliate orange, root of catclaw buttercup, tangerine seed, sappan wood, Szechwan Chinaberry fruit, white mustard seed, roasted ginger, dragon blood, combined spicebush root, rhizoma corydalis, rhizoma paridis, Indian mulberry, stir-fried frankincense, stir-fried myrrh, songaria cynomorium herb, common fenugreek seed and cinnamon. The medicine has the effects of clearing away heat and toxic materials, removing stasis and diminishing swelling, promoting blood circulation to remove blood stasis, warming the channels to relieve pain and diminishing inflammation and smoothing collateral. The medicine is mainly used for orchitis, epididymitis, testis and epididymis nodes, varicocele, testis prolapse and swelling, dull pain, cold and cool, radiating pain, prostatic hyperplasia, prostatitis and breast lump and hysteromyoma in gynaecology. The medicine has obvious therapeutic effect and no toxic or side effects.

The invention discloses an epididymal protease inhibitor protein monoclonal antibody and application thereof, which belong to the field of biological engineering. The monoclonal antibody is secreted by hybridoma preserved in China Center for Type Culture Collection (CCTCC) with the preservation number of C200849. The monoclonal antibody can be combined to the epididymal protease inhibitor protein Eppin protein of specific expression in a testis and epididymis tissue by specificity. The monoclonal antibody can be used for preparing male infertility diagnostic reagents, male contraceptives and anti-fertility study biological products.

The present invention discloses a method and kit for screening early-stage ovarian cancer. According to the method, early-stage ovarian cancer is screened through combined detection of keratanase protein (YKL-40), cancer antigen 125 (CA125) and human epididymis protein 4 (HE4). With the method, the early-stage ovarian cancer prediction accuracy can be improved.

The invention belongs to the field of biotechnology and medicine science, and provides sperm binding protein HEL-127 specifically expressed in human epididymis and a nucleic acid sequence for coding the protein. The invention also discloses a preparation method and application of the protein HEL-127. The protein is associated with the mature and defense function of sperms. The HEL-127 protein can be used for preparing medicaments of resisting infections and tumors, provides a novel molecular target for developing novel contraceptive medicine, carrying out aid diagnosis and treating infertility, and is favorable for researches on male reproductive function.

The invention relates to a method for improving the sperm motility with Sertoli cells as the trophoblast. The method mainly includes the steps of: (1) conducting isolation culture on Sertoli cells; (2) preparing a Sertoli cell trophoblast: conducting isolation culture on Sertoli cells for 2 days to form a cell monolayer, adding mitomycin C to perform treatment for 2h so as to prepare a Sertoli cell trophoblast; and (3) acquiring sperm from an epididymis of a mature male animal, adding the sperm into a culture system adopting Sertoli cells as the trophoblast, and performing co-culture on the sperm and Sertoli cells. The method provided by the invention adopts Sertoli cells as the trophoblast for in-vitro culture of sperms in testicle and epididymis, solves the problem of low in-vitro sperm motility, enhances the motility and survival rate of in-vitro sperms, and increases the fertilization capability of sperms.

The invention relates to an application of hydroxytyrosol in a medicine used for preventing and curing obesity. The hydroxytyrosol is capable of effectively reducing the increment of weight, as well as accumulation of epididymis fat and perirenal fat during the generating process of obesity. Moreover, the hydroxy tyrosol can reduce the contents of triglyceride, free fatty acid and hunger insulinum in blood; and in addition, the hydroxy tyrosol is capable of obviously restraining the generation of fatty liver, reducing the weight of the liver, and restraining the accumulation of the triglyceride and cholesterol in the liver. Therefore, the hydroxy tyrosol has the function of promoting fat metabolism and cholesterol metabolism, can effectively protect the liver function, and cure and prevent the occurrence of obesity, and belongs to a drug with high curative effect, safety and simplicity.

The invention discloses a kit for detecting human epididymis secretory protein 4 (HE4) by a double antibody sandwich method, which comprises a HE4 resistant mice monoclonal coated antibody, an enzyme-labeled HE4 rabbit polyclonal antibody and a recombinant HE4 albumen standard substance. The detection kit provided by the invention can be used for qualitatively or quantitatively detecting the HE4 level in sample and has the advantages that the detection method is convenient and practicable, the detection sensitivity and accuracy are high, the specificity is strong, and the detection kit can simultaneously rapidly detect bulk samples. Through quantitative determination of a tumor marker HE4, the kit can provides effective methods for early stage diagnosis and disease monitoring of ovarian cancer, and can play a vital role in clinical research.

The invention discloses a preparation method of a kit for early-to-mid rapid diagnosis of an ovarian cancer. The method comprises the following steps: 1. preparation of antigens: required antigens are prepared by adopting genes of galectin-3, transthyretin, carcinoma antigen-125 and human epididymis protein 4; 2, preparation of capture antibodies: the antigens are used for immunization of a mammal so as to obtain corresponding monoclonal antibodies; 3, preparation of detection antibodies: the antigens are used for immunization of the mammal so as to obtain corresponding polyclonal antibodies; 4, coating of the capture antibodies; 5, sealing and sample adding. The method has the benefits as follows: the ovarian cancer can be detected in the most comprehensive manner, the benign and malignant of a pelvic lump before menopause or after menopause can be better judged, differential diagnosis can be better performed on gynecology benign ovary lump and cyst as well as the ovarian cancer, the method has the characteristics of high sensitivity and high accuracy, and the sensitivity and accuracy can reach over 96 percent.

The subject invention relates to a new forward motility protein isolated from goat epididymal plasma and a process for the isolation of the said epididymal forward motility protein useful as a fertility promoter/blocker comprising subjecting goat epididymal plasma to multiple fractionations till purification to obtain purified new epididymal forward motility protein by the chromatographic method.

The present invention relates to a method of collecting semen from the epididymis or the testis of a lab animal and an artificial insemination method thereof. The present invention relates to a new applicable method in the animal production field using artificial insemination. Since the present invention solves the problems of the conventional method of artificial insemination of lab animals, it is excellent in terms of the pregnancy rate and productivity; is time-efficient; and is superior in economic and industrial respects by preventing a huge economic loss due to the costs for maintaining the mass breeding of animals.

The invention discloses novel application of 12-HEPE or pharmaceutically acceptable fatty acid thereof, and comprises the application of the 12-HEPE or the pharmaceutically acceptable fatty acid thereof in preparation of a medicine for treating NOA. The 12-HEPE or the pharmaceutically acceptable fatty acid thereof can play a therapeutic role by promoting proliferation and differentiation of the endogenous spermatogonium of a mouse damaged by busulfan, and experiments prove that recovery of the thickness and the integrity of the spermatogenic epithelium of the testis and the recovery of the sperm concentration of the tail of epididymiscan be observed through intragastric administration of the 12-HEPE into the NOA mouse induced by the busulfan, meanwhile, proliferation and differentiation of spermatogonium are promoted, expression of the paracrine factors of cells is supported, so that the 12-HEPE can be used as a new target for alleviating the spermatogenesis dysfunction of the testis of the NOA mouse, and a new method is provided for treating the non-obstructive azoospermia.

The invention relates to application of a hibiscus sabdariffa-green tea composition in relieving diet-induced metabolic disorders, and particularly provides an aqueous extract and a composition with the same. The aqueous extract is the aqueous extract of the mixture of hibiscus sabdariffa flower and green tea or is the mixture of a hibiscus sabdariffa aqueous extract and a green tea aqueous extract. It is found that the hibiscus sabdariffa aqueous extract and the green tea aqueous extract can remarkably relieve weight increment of the body and epididymis fat of a mouse induced by high-fat and high-sugar diet, lower blood glucose and blood fat, improve mouse liver fat deposition and increase insulin sensitivity, while has no obvious influence on the food intake and water intake of the mouse. It is also found that the hibiscus sabdariffa-longjing tea composition extracting solution can lower fatty liver by increasing liver fatty acid oxidation and lowering lipid synthesis; in addition, insulin resistance can be lowered in the way of restraining mTORC1/S6K.

A human epididymis protease profilin immunological contraception polypeptide is characterized in that the basic amino acid sequence of the polypeptide is amino terminal-LSEIKGVIVHRLEGVGGGMFVYGGCQGNNNN-carboxyl terminal; lysine can be used as linker to combine a plurality of the basic amino acid sequences together to compose multiple copies of series connection structure. The invention has the advantages that the product is easy to transport and preserve and the automated production of the product can be realized; the invention has effective immunogenicity and antigenicity, obvious immune effect, high effective and reversible contraceptive effect, safe use and no side effect.

The subject invention relates to a new forward motility protein isolated from goat epididymal plasma and a process for the isolation of the said epididymal forward motility protein useful as a fertility promoter/blocker comprising subjecting goat epididymal plasma to multiple fractionations till purification to obtain purified new epididymal forward motility protein by the chromatographic method.

The invention provides a Ddx5 gene deleted spermatogenesis disorder mouse model and a construction method thereof, and the inventor finds that a relationship exists between the Ddx5 gene and spermatogenic disorder or fertility dysfunction in research, the testicle volume of a male mouse with Ddx5 gene deletion is obviously reduced compared with that of a wild mouse. Moreover, the testicle of the mouse is free of germ cells, the epididymis is free of sperms, and any offspring cannot be obtained after the mouse is mated with a female mouse. Therefore, the Ddx5 gene deleted male mouse has the advantages of stability and thorough spermatogenic function inhibition, and is a good research model for male fertility disorders.

The invention belongs to the field of application of probiotics and particularly relates to application of a bacteroides intestinalis DSM 17393 strain in preparation of medicines for treating or preventing obesity. According to the invention, C57/BL6 male SPF-level mice in the same nest are divided into a control group and a strain filling group randomly, a test of filling a bacteroides intestinalis DSM 17393 strain suspension into the stomach is carried out once per day, then the mice are fed with high fat diet, and the test lasts for 7 weeks. The test verifies that the strain is very effective for controlling body weight and body fat, the content of subcutaneous fat of the groin is reduced by 15%, and the content of fat beside the epididymis is reduced by 20.9%. The bacteroides intestinalis DSM 17393 strain can be used for preparing medicines, pharmaceutical compositions, foods and feeds for treating or preventing obesity.

Novel GP-83 protein (human epididymis-associated disintegrin and metalloprotease 7 protein) and nucleic acid molecules are disclosed. The invention provides purified GP-83 protein, fusion protein, antigenic peptides, and anti-GP-83 antibodies. The invention also provides isolated GP-83 nucleic acid molecule, recombinant vectors containing the GP-83 nucleic acid molecule, host cells containing the recombinant vector, and non-human transgenic animals in which the GP-83 nucleic acid molecule has been introduced or interrupted.

The invention belongs to the field of biomedicine and health, and specifically relates to a unicellular algae fat-soluble substance for increasing male characteristics of males and applications thereof. The main active component of the fat-soluble substance is composed of natural carotenoid and oxidation derivatives thereof, or micro algae powder rich in the carotenoid and oxidation derivatives thereof. The natural fat-soluble mixed substance rich in carotenoid and oxidation derivatives thereof is extracted from unicellular algae, which is obtained though cell engineering large-scale culture. Furthermore, novel healthcare and medical products and biological preparations, which can improve or strengthen the male functions, are developed on the basis of feeding and verification on experiment animals, and the products and biological preparations are used to improve or strengthen certain male characteristics of males such as testis weight, stored sperm number and density in epididymis, and the like.