46 results about "Spermatogenic Cell" patented technology

The present invention provides a method of producing a non-human vertebrate that harbours germ cells having a desired gene transferred thereto, comprising injecting the desired gene to the testis of a non-human vertebrate wherein no tight junction exists between Sertoli cells to transfer the desired gene to germ cells, so as to obtain a non-human vertebrate that harbours the germ cells having the desired gene transferred thereto. Using the method of the present invention, even in animal species and lines for which in vitro transduction has been difficult to date, it is possible to obtain an individual harbouring germ cells, particularly spermatogonial stem cells, having a desired gene transferred thereto, at extremely high efficiency. Also, the fertility of the male to receive an injection of the gene is retained, compared to in vitro transduction of germ cells, because gene transfer is achieved without reducing the number of spermatogonial stem cells in the testis and transgenic sperms and transgenic animals can easily be prepared.

The invention discloses an application of hydrogen sulfide and donor thereof in improving the sperm quality and function, and relates to the field of reproduction. The hydrogen sulfide can be directly added into semen in vitro, and the activity of the sperms of clinical asthenospermia patients can be remarkably improved within short time. The hydrogen sulfide also can resist oxidative stress for the sperms cultured in vitro so as to reduce the stress injury of the sperms caused by manual operation and in-vitro culture and maintain the activity level of the sperms. The hydrogen sulfide also can be used for improving the dyszoospermia caused by infection, protecting the spermatogenic structure of testis, maintaining the completion of blood-testis barrier and the synthesis of androgen, reducing the inflammatory level in the testis tissue and the oxidative stress of spermatogenic cells, and reducing the apoptosis of spermatogenic cells caused by infection so as to maintain a normal spermatogenic process, keep the sperm quality and maintain normal fertility of male. The invention opens up a new application field of hydrogen sulfide and provides a significant reference to solving the human dysgenesis and improving the assisted reproductive technology.

The invention provides an application of C3G in preparation of an antagonist for resisting oxidation system damage caused by 3-MCPD contamination. The invention proves that C3G improves the conditions of reduced feeding and slow growth of rats caused by 3-MCPD contamination; the C3G can effectively antagonize rat anti-oxidation system damage caused by 3-MCPD contamination and activate and regulate BTB structure functions of supporting cells by inhibiting an MAPK signal channel, so that spermatogenic epithelium damage caused by 3-MCPD is relieved, the stability of a spermatogenic microenvironment is maintained, division and differentiation of spermatogenic cells are regulated, and spermatogenic disorder is improved. Meanwhile, C3G can improve sperm activity by adjusting sperm energy metabolism related enzymes. Rat sex hormone secretion disorder caused by 3-MCPD contamination is regulated, and the normal level of sex hormone in serum and androgen in testis internal environment is maintained. The application of C3G in preparation of the antagonist for resisting oxidation system damage caused by the 3-MCPD contamination provides a theoretical basis for nutrition intervention of anthocyanin on 3-MCPD reproductive toxicity in the future.

The invention provides an RNAi (ribonucleic acid interference) interference segment and RNAi interference vector for interfering with Zfx gene in animal testicle spermatogenic cells. The RNAi interference vector has the RNAi interference segment; and the RNAi interference segment comprises the following sequence: TGAGG CAGAT GTATC TGAAA TTCAA GAGATTTCAG ATACA TCTGC CTCTT TTTTC or TGCAG AGAAG GCCATTGAAT TTCAA GAGAA TTCAA TGGCC TTCTC TGCTT TTTTC. The RNAi interference segment and RNAi interference vector provided by the invention can interfere with the Zfx gene in animal testicle spermatogenic cells, so that the Zfx gene expression is lowered to indirectly influence the fertilization capacity of the X sperm, thereby achieving the goal of controlling the gender of the pig offsprings.

The invention relates to a radioprotective medicament, and particularly relates to a radioprotective medicament prepared by using traditional Chinese medicine as raw material herbs. The medicament is used to treat and prevent problems of reproductive system radiation hazard, and comprises the following herbs by mass: 90-150 parts of medlar, 90-150 parts of semen cuscutae, 40-70 parts of cistanche, 40-70 parts of raspberry, 25-40 parts of prepared rehmannia roots. The medicament of the invention can be prepared into any commonly used oral dosage form by routine traditional Chinese medicine preparation methods, such as pills, powder, tablets, capsules, oral liquid, etc. The medicament has the efficacy of tonifying kidney, replenishing essence, and tonifying qi, and has obvious effect on ionizing radiation resistance and spermatogenic cell protection.

The invention discloses a preparation method of a slide for specific spermatogenic cells at a development stage, and the method comprises the following steps: removing the envelope of a testis tissue which is taken from an animal, dispersing contorted senimiferous tubules, determining staging of a part corresponding to a spermatogenic epithelium wave mode according to the light transmission difference of the contorted senimiferous tubules under a dissecting microscope, then selecting an interesting part section to prepare the slide, and fixing the slides by 1wt% of paraformaldehyde solution after being frozen in liquid nitrogen, thus preparing the required slide for the specific spermatogenic cells. The method has the maximum advantage that the living cellular level of related molecules in the spermatogenic process is detected in vitro, complete, objective and precise experimental results can be obtained, and the results reflect expression features of a specific gene product of the spermatogenic cells at the development stage.

The invention relates to a method for inducing skin stem cells to differentiate into sperms. The method comprises the following steps of firstly, co-culturing the skin stem cells of a mouse and fibroblast treated by mitomycin C; inducing to differentiate into PGCs (primordial germ cells); then, reversely injecting PGC cell mixing liquid into a testis seminiferous tubule through an efferent ductule of a recipient mouse; after developing for one month in the testis of the recipient mouse, fetching out the testis of the recipient mouse; after HE staining analysis, finding the recovery of spermatogenesis in the seminiferous tubule, emerging of cells with typical sperm morphology, and expression of GFP and MVH of spermatogenic cells in the seminiferous tubule with recovery of spermatogenesis. The method has the advantages that the injury to a body is little, the limitation of ethical issue is avoided, the skin stem cells are effectively induced to be differentiated into sperm cells, the differentiation efficiency is high, the sperm cells obtained by differentiation have typical sperm morphology, and the specific gene of the sperm cell is expressed.