54 results about "Oxidative injury" patented technology

The invention discloses application of walnut peptide in preparation of a medicine for treating or preventing cognitive impairment of diabetes mellitus. The preparation process of the walnut peptide is as follows: after walnut protein is subjected to enzymolysis by alkaline protease, the molecular weight cut-off is 1t; and separating by using a 3kDa ultrafiltration tube to obtain the walnut peptide. The walnut peptide can effectively reduce the blood sugar level of a diabetic model mouse, regulate the disorder effect of blood fat, relieve brain tissue oxidative damage and hippocampal neuroinflammation damage of the diabetic model mouse, and improve learning and memory damage. The compound can be used for preparing medicines for treating or preventing cognitive impairment of diabetes mellitus.

Disclosed are therapeutic "brain boost" combinations of agents and pharmaceutical kits and compositions thereof, such as combinations of serotonergic (5-HT 2A receptor agonist) psychedelics, with mitochondrial energy enhancers, such as metabolic cofactors and metabolic coenzymes, in some embodiments together with antioxidants and glucose regulators. In some embodiments, the combinations are used in methods of enhancing energy, such as increasing cellular energy production or metabolism, and for optimizing glucose levels and protecting cells from oxidative damage. In some embodiments, the combinations have applications as longevity treatments, for end-of-life care, for the treatment and prevention of mental health conditions, and for the betterment of mental functioning and overall health and wellbeing, such as by heightening consciousness and enhancing cognitive function.

The application discloses a kind of compositions for treating radiation enteropathy and application thereof, belong to medical technology field.The composition includes the following components: anemarrhena asphodeloides B4, obakunine, berberine, aesculetin, fraxinellone and fraxinellin.The composition has no toxic side effects, has good safety, can reduce the inflammatory infiltration of intestinal epithelial cells, reduce intestinal epithelial cell apoptosis, protect intestinal barrier and protect intestinal oxidative damage by regulating NF-κB, Caspase 9 / 3 and NRF2 pathway, and then multi-component, multi-pathway jointly play the efficacy of treating radiation enteropathy.The problem that there is no effective drug for treating radiation enteropathy in the prior art is solved.

The invention discloses an application of daidzin to preparation of a medicine and a potency verification method of daidzin, the medicine is applied to improvement of age-related macular degeneration, and the potency verification method comprises in-vitro verification and in-vivo verification; the protection effect of daidzin on oxidative damage caused by SIN-1 is evaluated through an in-vitro experiment, and the protection effect is specifically shown as increase of cell activity and reduction of MDA content; in-vivo verification is completed by means of a rat model, a visual placement reaction experiment is formed, a rat visual function system is evaluated, the MDA content of rat eye peripheral blood is detected, and it is clear that the daidzin can obviously relieve AMD damage, that is, the daidzin has an obvious improvement effect on AMD; and daidzin is good in safety and low in improvement and treatment cost on age-related macular degeneration, and meets the current clinical requirements.

The present invention relates to a composition for preventing and alleviating or treating glaucoma and macular degeneration, containing a Centella asiatica extract. Particularly, the composition increases the glucose metabolism efficiency of cells so as to increase the survival rate of retinal neurons and pigment epithelial cells and protects cells from oxidative damage caused by A2E, thereby being effectively usable as a composition for eye health and for preventing and alleviating or treating glaucoma and macular degeneration.

Compositions for the treatment of acute manifestations because of oxidative injury by reactive oxygen species (ROS) and chronic development of fibrosis are provided. Compositions of the present invention include perfluorocarbon, hydrogen and oxygen gas, N-acetylcysteine, and glycine to counter robust oxidative responses unleashed following injury from both mechanical and non-mechanical conditions. Methods are also disclosed for delivery to challenging locations (olfactory cleft, spinal canal) and in hostile conditions (post trauma brain, spine, and upper and lower airways), with the invention's products "calming" inflamed tissue by neutralizing ROS in both acute and later complications. Active pharmaceutical ingredient(s) can also be employed to provide both acute and chronic benefit, and such active ingredients themselves can be augmented by the composition, enabling increased drug uptake and oxygenation. The present invention provides broad relief in many conditions for both human and companion subjects.

A protein polymer and a production process therefor. The protein polymer is obtained by means of stimulating MSCs, lysing same, and separating and purifying same. The protein polymer has a strong capability to repair oxidative damage, and thus can reduce the range of cerebral infarction, improve neural functions, inhibit the level of neuroinflammation, and increase the number of surviving neurons.

The invention relates to a composition for preventing and treating alcoholic liver injury and application thereof. The composition mainly comprises the following active ingredients: wheat peptide, soft-shelled turtle peptide and kudzuvine root powder. According to the invention, zebra fish is used as a model organism, ethanol exposure treatment is utilized to induce and establish an alcoholic liver injury model, and the alcoholic liver injury is identified by means of apparent histomorphology observation, biochemical index detection, gene expression quantity analysis and the like. Two bioactive peptides (wheat peptides and soft-shelled turtle peptides) screened from six bioactive peptides and five plant extracts are compounded with one plant extract (radix puerariae powder), and the effects of dispelling the effects of alcohol and protecting the liver of the compound are explored from the aspects of enteric microbiomics and non-targeted metabonomics. Researches show that the intervention of the compound significantly improves the hepatomegaly induced by alcohol, and has a synergistic effect in the aspects of preventing hepatocyte injury (improving ALDH activity) and oxidative injury (improving SOD activity) caused by alcohol.

The invention discloses a system for monitoring the influence of atorvastatin on mitochondrial functions in real time, and the system comprises a biosensor module which is used for monitoring biomarkers related to the mitochondrial functions and glycometabolism in blood in real time; the micro-fluidic chip is used for rapidly separating and enriching cells or specific components in blood; the optical detection module monitors the active oxygen level of mitochondria in real time; the data processing and transmission module processes and analyzes the collected data in real time; the equipment terminal receives the processed data and displays the data on a user interface in real time; the analysis and early warning module analyzes the monitoring data in real time and provides early warning and personalized suggestions for diabetes mellitus; the database module is used for storing monitoring data and historical records; through a biosensor, optical detection, a micro-fluidic chip and the like, after a patient takes atorvastatin, indexes of blood sugar, insulin and the like in a blood sample, and mitochondrial functions and oxidative damage indexes of active oxygen level, mitochondrial membrane potential and the like are detected.

The application belongs to the field of nanomaterials and biotechnology, and particularly relates to application of non-ROS-dependent photoenzyme in photocatalysis and Nrf2 activation. The preparation method of the non-ROS-dependent photoenzyme is as follows: chloroauric acid is added into a mixed solution of 6-azido-2-thiothymine and sodium hydroxide, and the reaction is stirred at 25-30 DEG C to prepare nanometer photoenzyme; the molar ratio of 6-azido-2-thiothymine to chloroauric acid is (2.1-2.6):1. The non-ROS-dependent nanometer photoenzyme provided by the application has the advantages of simple and efficient synthesis method, ultrahigh photocatalytic activity, non-ROS-dependent catalytic mechanism, excellent biocompatibility, and the ability to realize intracellular light-controlled activation of Nrf2, and solves the problems of ROS-dependent oxidative damage, uncontrollable Nrf2 activation, and over-activation of signal pathways.

The invention belongs to the technical field of fungal polysaccharide application, and particularly relates to a yellow-rose yellow-meat boletus polysaccharide as well as a preparation method and application thereof. The preparation method comprises the following steps: extracting crude polysaccharide from boletus rosea sporocarp through a water extraction and alcohol precipitation method, and then carrying out deproteinization, decoloration and DEAE-52 cellulose column chromatography separation and purification to obtain the purified polysaccharide BPP-1 of the boletus rosea water component. CCl4 is selected as an inducer of oxidative stress injury of the MIHA cells to construct a hepatic cell oxidative injury model, and the model is utilized to evaluate the protective effect of BPP-1 on CCl4-induced MIHA cell hepatic injury. The result shows that the BPP-1 reduces the release of ALT and AST, improves the GSH level and the SOD enzyme activity at the same time, inhibits the generation of a lipid peroxidation product MDA, and effectively reduces the damage of CCl4 to liver cells. The results show that BPP-1 has a certain protection effect on CCl4-induced hepatocyte injury, and BPP-1 may be a potential antioxidant and can alleviate oxidative injury of liver.

The invention provides a bacillus amyloliquefaciens and application thereof. The preservation number of the bacillus amyloliquefaciens is CGMCC (China General Microbiological Culture Collection Center) NO.28723. The bacillus amyloliquefaciens can be used for The bacillus amyloliquefaciens disclosed by the invention can be used for preparing animal feed, and has the advantages of being low in addition amount, capable of relieving serum oxidative injury and intestinal injury of animals, protecting livers of the animals and the like.

The invention discloses a lactobacillus crispatus SX-E770 and application of the lactobacillus crispatus SX-E770 in preparation of products for reducing blood glucose and / or resisting oxidation, the preservation number of the lactobacillus crispatus SX-E770 is CCTCC NO: M 2025033, and the lactobacillus crispatus SX-E770 is preserved in China Center for Type Culture Collection on January 6, 2025. The novel lactobacillus crispatus strain SX-E770 provided by the invention simultaneously has excellent blood glucose reducing and oxidation resisting capabilities, can effectively reduce the content of glucose in a body, is good in safety, and has wide application prospects in the aspect of developing products such as probiotic preparations and medicines for regulating the blood glucose level, relieving oxidative damage and preventing and treating diabetes mellitus.

The invention relates to the technical field of nursing materials, and discloses eye washing liquid for relieving eye fatigue and a preparation method thereof.The eye washing liquid is prepared from trehalose, taurine, sodium gualenate, sodium hyaluronate, ergothioneine, vitamin B12, vitamin B6, xanthophyll, mint, a humectant, a bacteriostatic agent, a chelating agent, a pH regulator and deionized water. Through the synergistic effect of the first synergistic composition and the second synergistic composition, retina cell injury induced by blue light can be effectively repaired, the repairing effect is superior to that of simple addition of a single component, blue light oxidative injury is repaired through a multi-target synergistic mechanism, a three-layer structure of a tear film lipid layer, a water layer and a mucoprotein layer can be reconstructed, and the repairing effect is good. According to the present invention, the tearable film stability is significantly improved, the excellent protection and repair effect is provided, the compatibility of the components is good, the product stability is high, and the multiple effects of ocular surface cleaning, asthenopia relieving, conjunctival congestion reducing and blue light oxidation damage preventing are provided.

The invention relates to the technical field of nano biomedicine, and discloses an ultramicro-diameter cerium dioxide nano-enzyme, engineered dental pulp mesenchymal stem cells of the ultramicro-diameter cerium dioxide nano-enzyme and a method for improving the stability of exosomes. The ultramicro-diameter cerium dioxide nano-enzyme is prepared from citric acid and cerium nitrate hexahydrate according to the mass ratio of 2: (1.17-3.34), the preparation method comprises the following steps: mixing citric acid and cerium nitrate hexahydrate according to a mass ratio, carrying out intermittent ultrasonic treatment after mixing, and centrifuging. The diameter of the prepared citric acid and cerium nitrate hexahydrate is 1-5nm. The prepared ultramicro-diameter cerium dioxide nano-enzyme and dental pulp mesenchymal stem cells are incubated together to construct the engineered dental pulp mesenchymal stem cells, and the constructed E-DPSC can effectively maintain the redox balance in the cells, protect the cells from oxidative damage, enhance the osteogenesis and chondrogenesis differentiation capacities of the cells and improve the osteogenesis and chondrogenesis differentiation capacities of the cells. And the room-temperature physical stability of the exosome secreted by the constructed engineered dental pulp mesenchymal stem cells is effectively improved.

The invention belongs to the technical field of biomedicine, and particularly relates to nano albumin paclitaxel particles as well as a preparation method and application thereof. According to the invention, DCC and NHS are used as condensing agents, so that the conjugate HSA-RA with relatively high in-vivo stability can be obtained; hSA-RA and PTX are self-assembled according to a specific ratio, so that the effect of synergistically inhibiting cancer cells by PTX and RA can be improved, and the effective release rate of the medicine can also be improved. The nano albumin paclitaxel particle provided by the invention not only enhances the killing effect of paclitaxel on living tumor cells, but also has an obvious improvement effect on cervical cancer cell inflammation and oxidative damage, so that by inhibiting the expression of CYP3A4 and CYP2C8, the nano albumin paclitaxel particle has obvious and positive effects on treatment and prognosis of cervical cancer.

Pharmacological experiment results show that in a chronic alcoholic intestinal injury model, total lignans of schisandra chinensis can reduce histological injury of intestinal tracts of mice, relieve oxidative injury of small intestines, improve tight connection of the intestinal tracts through a RhoA / ROCK1-MLCK-MLC pathway, improve the intestinal tracts and improve the intestinal tracts, so that the total lignans of schisandra chinensis can be used for preventing and treating chronic alcoholic intestinal injury. The composition can improve intestinal wall villus integrity, reduce intestinal permeability and regulate intestinal flora disorder, so that alcoholic intestinal injury is improved.

The invention discloses application of a known compound, namely a thiazole carboxamide derivative (with the CAS number of 933886-42-1), in preparation of a medicine for treating acute lung injury / acute respiratory distress syndrome, the compound is an SLC39A1 agonist, zinc ions entering cells can be effectively increased, and by reducing the expression quantity of lung tissue inflammation genes IL-1beta and TNF-alpha of mice, the lung tissue inflammation genes IL-1beta and TNF-alpha can be effectively inhibited, so that the acute lung injury / acute respiratory distress syndrome can be effectively inhibited, and the acute lung injury / acute respiratory distress syndrome can be effectively inhibited. Therefore, lung cell infiltration in ALI / ARDS mice is reduced, oxidative damage and tissue damage of lung tissue, inflammatory factor expression and cell connection are improved, and a new choice is provided for treatment of ALI / ARDS.

The invention relates to a small molecule compound composition and application thereof, and belongs to the technical field of biology. The small molecule compound composition provided by the invention comprises NU7441, GW9662 and D609 or stereoisomers thereof. It is found for the first time that the small molecule compound combination can effectively inhibit oxidative damage caused by hypertonic to corneal epithelial cells, and the survival rate of the corneal epithelial cells is remarkably increased. Animal in-vivo experiments show that compared with a control group, an experimental group which is subjected to injection of scopolamine dry eye model mice and is subjected to eye drop of the small molecule compound combination eye drop at the same time has the advantages that corneal defects are obviously reduced, tear secretion is increased, the small molecule compound combination can effectively prevent corneal epithelium injury, the drug effect is relatively high, and no obvious drug toxicity exists; the small molecule compound combination may become a strong and effective medicine for clinically treating dry eye in the future.

The invention belongs to the technical field of biological medicines, and particularly relates to a composition for relieving oxidative stress injury of intestinal tracts and application of the composition. The liquorice extract, the pueraria flower extract and the citrus aurantium flower extract are compounded according to a certain proportion, the antioxidant and anti-inflammatory effects of the liquorice extract are complementary with the powerful antioxidant and barrier repair functions of the pueraria flower extract and the citrus aurantium flower aqueous extract, and the overall protection effect is enhanced. The synergistic effect not only can more comprehensively relieve intestinal injury caused by oxidative stress, but also can improve the capability of preventing and repairing oxidative injury, so that a more effective relieving effect is achieved. On the basis, the wild buckwheat rhizome extract is further compounded, so that the overall protection effect can be further enhanced, the cell survival rate after H2O2 damage is improved, and ROS generation is inhibited. Results of the embodiment show that the composition provided by the invention can improve the cell survival rate after H2O2 injury, inhibit the generation of ROS (reactive oxygen species) and effectively relieve the oxidative stress injury of the intestinal tract.

The invention belongs to the technical field of nano materials and biology, and particularly relates to application of a non-ROS dependent luminous energy enzyme in photocatalysis and Nrf2 activation. The preparation method of the non-ROS dependent luminous energy enzyme comprises the following steps: adding chloroauric acid into a mixed solution of 6-aza-2-thiothymine and sodium hydroxide, and stirring for reaction at 25-30 DEG C to prepare the nano luminous energy enzyme; the molar ratio of the 6-aza-2-thiothymine to the chloroauric acid is (2.1 to 2.6): 1. The non-ROS-dependent nano light energy enzyme provided by the invention has the advantages of simple and efficient synthesis method, ultrahigh photocatalytic activity, non-ROS-dependent catalytic mechanism and excellent biocompatibility, can realize intracellular light-operated activation of Nrf2, and solves the problems of ROS-dependent oxidative damage, aggravation, uncontrollable Nrf2 activation, excessive activation of signal channels and the like.

The invention discloses application of sodium butyrate in preparation of a medicine for preventing or treating sepsis-related acute kidney injury, and relates to the technical field of pharmacy. The invention relates to an application of sodium butyrate in preparation of a medicine for preventing or treating sepsis-related acute kidney injury. The invention finds and verifies that sodium butyrate improves the kidney function of sepsis-related acute kidney injury, alleviates cell inflammatory response and oxidative injury and inhibits ferroptosis. The sodium butyrate inhibits ferroptosis by increasing the expression of VDR and GPX4 proteins.

The invention discloses an anti-aging composition containing a retinol derivative as well as a preparation method and application of the anti-aging composition, and belongs to the technical field of pharmaceutical preparations. The anti-aging composition comprises the following components in percentage by weight: 15-22% of an oil phase; 10 to 15% of polyol; 7.5-12% of a compound emulsifier; 0.5 to 2% of a retinol derivative; and the balance of deionized water. Wherein the compound emulsifier is prepared from 2 to 4 percent of inulin stearyl carbamate, 5 to 7 percent of behenyl alcohol polyether and 0.5 to 1.0 percent of behentrimethylammonium methyl sulfate. Through optimization of an emulsifier system, the problems of low stability and low permeability of a retinol derivative are solved, in addition, on the basis of the components of the anti-aging composition, the following raw materials in percentage by weight are added: 0.5-1.5% of a flavonoid compound, 0.5-1.5% of an emulsifier, 0.5-1.5% of an antioxidant and the balance of water. 0.05 to 0.1 percent of EDTA (Ethylene Diamine Tetraacetic Acid)- 0.15 to 0.3 percent of phenoxyethanol; 0.05 to 0.2 percent of ethylhexylglycerin; the external repair preparation capable of specifically solving the problem of skin oxidative injury induced by ultraviolet rays is prepared and obtained, the formula is mild and stable, the preparation process is simple, and the application prospect is wide.

The invention relates to casein peptide hydrolysate and application thereof in preparation of drugs for preventing or treating nerve aging, and belongs to the technical field of biological medicines. The casein hydrolysate is obtained by fermenting a casein solution with the probiotic bifidobacterium longum BNCC 185354, and the obtained casein hydrolysate has superoxide anion free radical scavenging capacity, can prolong the telomere length of the hippocampus of a subacute aging model mouse and relieve DNA oxidative damage of the hippocampus of the mouse. The brand-new casein peptide with both brain-accessible activity and biological activity is further screened from casein hydrolysate, free radicals can be removed, nerve cell inflammation can be eliminated, and therefore nerve aging is delayed. The casein peptide and the casein hydrolysate provided by the invention can be applied to preparation of drugs for preventing and / or treating nerve aging, and have good application prospects.

The invention relates to application of vinca in preparation of a beta-amyloid protein aggregation inhibitor and an anti-Alzheimer disease preparation, and belongs to the technical field of biological medicines. According to the invention, experiments show that Venoclara can effectively inhibit beta-amyloid protein (A beta) aggregation, change the morphology of beta-amyloid protein aggregation, and prevent and slow down the transformation of beta-amyloid protein to fibrous morphology; furthermore, the invention also finds that the Venoclara can effectively inhibit the cytotoxicity induced by aggregates formed in the beta-amyloid protein aggregation process, and meanwhile, the Venoclara does not cause oxidative damage to cells and has good biological safety when being independently used. The invention provides an inhibitor capable of effectively inhibiting beta-amyloid protein aggregation for the prior art, and provides a new drug resource for research and development of drugs for treating amyloid protein related diseases (such as Alzheimer's disease, cerebral amyloid angiopathy and the like).

The present invention discloses the application of Hamaudol in improving acute kidney injury and intestinal barrier function, and belongs to the field of drug development technology. The present invention finds that by intraperitoneally injecting the Chinese medicine monomer Hamaudol into mice, the intestinal barrier function can be significantly improved, the expression of tight junction proteins ZO-1 and Occludin in the intestinal tract of mice can be increased, and the serological indicators of acute kidney injury can be reduced at the same time, the pathological changes of renal tubular epithelial cells in mice with acute kidney injury can be improved, the expression of inflammatory factors in the kidney can be downregulated, and apoptosis and oxidative damage can be improved. Thus, acute kidney injury is prevented and treated, and a new target is provided for the treatment of acute kidney injury.

The invention discloses refreshing and anti-fatigue food and a preparation process thereof, and relates to the technical field of food. The coffee bean beverage comprises active components and auxiliary components, the active components comprise, by mass, 20-30% of coffee bean extract, 5-10% of ginsenoside, 8-15% of taurine and 2-5% of vitamin B complex, and the auxiliary components comprise, by mass, 10-20% of natural sweetener, 15-25% of dietary fiber and 5-8% of slow-release carrier. The food has the beneficial effects that multiple action mechanisms are formed through chlorogenic acid, ginsenoside, taurine and vitamin B family, the problems of oxidative damage, energy metabolism disorder and nerve fatigue can be solved at the same time, the fatigue relieving time requirement is accurately matched through the design of a composite carrier, the action time is prolonged to 6-8 hours, and the fatigue relieving effect is improved. According to the preparation process, supercritical extraction and low-temperature ultrasonic waves are combined, so that the activity of thermosensitive components can be retained to the maximum extent, and unification of high-activity retention and industrial efficiency is realized.

The invention discloses application of spermidine in preparation of a medicine for treating liver injury caused by iron overload, and belongs to the technical field of biological medicine. Experimental verification shows that spermidine can be applied to relieving liver iron ion deposition caused by iron dextran, reducing MDA content, effectively improving liver SOD and CAT enzyme activity and total oxidation resistance, and effectively solving the problems of liver oxidative damage and liver inflammation gene expression up-regulation caused by iron overload. Therefore, spermidine can be developed into a medicine for preventing and treating liver oxidative injury caused by iron overload. The invention finds a new effective substance for prevention and treatment of iron overload.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to sperm-producing decoction as well as a preparation method, application and a product thereof. The sperm-producing decoction is prepared from the following raw materials in parts by weight: 5 to 15 parts of radix salviae miltiorrhizae, 5 to 15 parts of rhizoma chuanxiong, 5 to 15 parts of radix angelicae sinensis, 10 to 25 parts of herba epimedii, 10 to 25 parts of fructus cnidii, 10 to 25 parts of semen cuscutae, 5 to 15 parts of fructus rubi and 5 to 15 parts of fructus schisandrae chinensis. The spermatogenic decoction can improve testis injury caused by an anti-inflammatory and anti-immune drug tripterygium wilfordii hormone, improve oxidative injury of testis tissues, regulate the expression level of antioxidant stress related proteins Nrf2 and HO-1, improve the phenomena of testis diminishment, seminiferous tubule damage, sperm number reduction, sperm motility weakening, testis spermatogenic cell apoptosis and the like, and can be used for preparing the anti-inflammatory and anti-immune drug. And the expression level of apoptosis-related proteins Bcl-2 and Bax of the testicular spermatogenic cells is regulated.

The invention provides establishment of a cat kidney cell oxidative stress injury model and application of the cat kidney cell oxidative stress injury model in treatment of cat kidney oxidative injury. In the research, cat kidney cells (F81 cells) are taken as a research model, and a cat kidney cell oxidative stress injury model is successfully constructed. On the basis, the anti-oxidation effect of the curcumin on the F81 cells is screened and verified, and functional genes and related pathways, which influence the F81 cells, of the curcumin are analyzed through transcriptome, so that a certain theoretical basis is provided for application of the curcumin in functional foods for pet cats and related fields of pets for reference; the application verifies that the curcumin has a good treatment effect on kidney-related diseases of pet cats and has an obvious synergistic effect on prevention and treatment of peroxidation stress of kidney injury of the pet cats in combination with CXCL8 neutralizing antibody medication, the effect is obviously improved, and the curcumin is superior to a single medication group.