36 results about "Oxidative injury" patented technology

The application discloses a kind of compositions for treating radiation enteropathy and application thereof, belong to medical technology field.The composition includes the following components: anemarrhena asphodeloides B4, obakunine, berberine, aesculetin, fraxinellone and fraxinellin.The composition has no toxic side effects, has good safety, can reduce the inflammatory infiltration of intestinal epithelial cells, reduce intestinal epithelial cell apoptosis, protect intestinal barrier and protect intestinal oxidative damage by regulating NF-κB, Caspase 9 / 3 and NRF2 pathway, and then multi-component, multi-pathway jointly play the efficacy of treating radiation enteropathy.The problem that there is no effective drug for treating radiation enteropathy in the prior art is solved.

Compositions for the treatment of acute manifestations because of oxidative injury by reactive oxygen species (ROS) and chronic development of fibrosis are provided. Compositions of the present invention include perfluorocarbon, hydrogen and oxygen gas, N-acetylcysteine, and glycine to counter robust oxidative responses unleashed following injury from both mechanical and non-mechanical conditions. Methods are also disclosed for delivery to challenging locations (olfactory cleft, spinal canal) and in hostile conditions (post trauma brain, spine, and upper and lower airways), with the invention's products "calming" inflamed tissue by neutralizing ROS in both acute and later complications. Active pharmaceutical ingredient(s) can also be employed to provide both acute and chronic benefit, and such active ingredients themselves can be augmented by the composition, enabling increased drug uptake and oxygenation. The present invention provides broad relief in many conditions for both human and companion subjects.

A protein polymer and a production process therefor. The protein polymer is obtained by means of stimulating MSCs, lysing same, and separating and purifying same. The protein polymer has a strong capability to repair oxidative damage, and thus can reduce the range of cerebral infarction, improve neural functions, inhibit the level of neuroinflammation, and increase the number of surviving neurons.

The invention discloses a system for monitoring the influence of atorvastatin on mitochondrial functions in real time, and the system comprises a biosensor module which is used for monitoring biomarkers related to the mitochondrial functions and glycometabolism in blood in real time; the micro-fluidic chip is used for rapidly separating and enriching cells or specific components in blood; the optical detection module monitors the active oxygen level of mitochondria in real time; the data processing and transmission module processes and analyzes the collected data in real time; the equipment terminal receives the processed data and displays the data on a user interface in real time; the analysis and early warning module analyzes the monitoring data in real time and provides early warning and personalized suggestions for diabetes mellitus; the database module is used for storing monitoring data and historical records; through a biosensor, optical detection, a micro-fluidic chip and the like, after a patient takes atorvastatin, indexes of blood sugar, insulin and the like in a blood sample, and mitochondrial functions and oxidative damage indexes of active oxygen level, mitochondrial membrane potential and the like are detected.

The application belongs to the field of nanomaterials and biotechnology, and particularly relates to application of non-ROS-dependent photoenzyme in photocatalysis and Nrf2 activation. The preparation method of the non-ROS-dependent photoenzyme is as follows: chloroauric acid is added into a mixed solution of 6-azido-2-thiothymine and sodium hydroxide, and the reaction is stirred at 25-30 DEG C to prepare nanometer photoenzyme; the molar ratio of 6-azido-2-thiothymine to chloroauric acid is (2.1-2.6):1. The non-ROS-dependent nanometer photoenzyme provided by the application has the advantages of simple and efficient synthesis method, ultrahigh photocatalytic activity, non-ROS-dependent catalytic mechanism, excellent biocompatibility, and the ability to realize intracellular light-controlled activation of Nrf2, and solves the problems of ROS-dependent oxidative damage, uncontrollable Nrf2 activation, and over-activation of signal pathways.

The invention belongs to the technical field of fungal polysaccharide application, and particularly relates to a yellow-rose yellow-meat boletus polysaccharide as well as a preparation method and application thereof. The preparation method comprises the following steps: extracting crude polysaccharide from boletus rosea sporocarp through a water extraction and alcohol precipitation method, and then carrying out deproteinization, decoloration and DEAE-52 cellulose column chromatography separation and purification to obtain the purified polysaccharide BPP-1 of the boletus rosea water component. CCl4 is selected as an inducer of oxidative stress injury of the MIHA cells to construct a hepatic cell oxidative injury model, and the model is utilized to evaluate the protective effect of BPP-1 on CCl4-induced MIHA cell hepatic injury. The result shows that the BPP-1 reduces the release of ALT and AST, improves the GSH level and the SOD enzyme activity at the same time, inhibits the generation of a lipid peroxidation product MDA, and effectively reduces the damage of CCl4 to liver cells. The results show that BPP-1 has a certain protection effect on CCl4-induced hepatocyte injury, and BPP-1 may be a potential antioxidant and can alleviate oxidative injury of liver.

The invention relates to the technical field of nursing materials, and discloses eye washing liquid for relieving eye fatigue and a preparation method thereof.The eye washing liquid is prepared from trehalose, taurine, sodium gualenate, sodium hyaluronate, ergothioneine, vitamin B12, vitamin B6, xanthophyll, mint, a humectant, a bacteriostatic agent, a chelating agent, a pH regulator and deionized water. Through the synergistic effect of the first synergistic composition and the second synergistic composition, retina cell injury induced by blue light can be effectively repaired, the repairing effect is superior to that of simple addition of a single component, blue light oxidative injury is repaired through a multi-target synergistic mechanism, a three-layer structure of a tear film lipid layer, a water layer and a mucoprotein layer can be reconstructed, and the repairing effect is good. According to the present invention, the tearable film stability is significantly improved, the excellent protection and repair effect is provided, the compatibility of the components is good, the product stability is high, and the multiple effects of ocular surface cleaning, asthenopia relieving, conjunctival congestion reducing and blue light oxidation damage preventing are provided.

Pharmacological experiment results show that in a chronic alcoholic intestinal injury model, total lignans of schisandra chinensis can reduce histological injury of intestinal tracts of mice, relieve oxidative injury of small intestines, improve tight connection of the intestinal tracts through a RhoA / ROCK1-MLCK-MLC pathway, improve the intestinal tracts and improve the intestinal tracts, so that the total lignans of schisandra chinensis can be used for preventing and treating chronic alcoholic intestinal injury. The composition can improve intestinal wall villus integrity, reduce intestinal permeability and regulate intestinal flora disorder, so that alcoholic intestinal injury is improved.

The invention discloses application of a known compound, namely a thiazole carboxamide derivative (with the CAS number of 933886-42-1), in preparation of a medicine for treating acute lung injury / acute respiratory distress syndrome, the compound is an SLC39A1 agonist, zinc ions entering cells can be effectively increased, and by reducing the expression quantity of lung tissue inflammation genes IL-1beta and TNF-alpha of mice, the lung tissue inflammation genes IL-1beta and TNF-alpha can be effectively inhibited, so that the acute lung injury / acute respiratory distress syndrome can be effectively inhibited, and the acute lung injury / acute respiratory distress syndrome can be effectively inhibited. Therefore, lung cell infiltration in ALI / ARDS mice is reduced, oxidative damage and tissue damage of lung tissue, inflammatory factor expression and cell connection are improved, and a new choice is provided for treatment of ALI / ARDS.

The invention belongs to the technical field of nano materials and biology, and particularly relates to application of a non-ROS dependent luminous energy enzyme in photocatalysis and Nrf2 activation. The preparation method of the non-ROS dependent luminous energy enzyme comprises the following steps: adding chloroauric acid into a mixed solution of 6-aza-2-thiothymine and sodium hydroxide, and stirring for reaction at 25-30 DEG C to prepare the nano luminous energy enzyme; the molar ratio of the 6-aza-2-thiothymine to the chloroauric acid is (2.1 to 2.6): 1. The non-ROS-dependent nano light energy enzyme provided by the invention has the advantages of simple and efficient synthesis method, ultrahigh photocatalytic activity, non-ROS-dependent catalytic mechanism and excellent biocompatibility, can realize intracellular light-operated activation of Nrf2, and solves the problems of ROS-dependent oxidative damage, aggravation, uncontrollable Nrf2 activation, excessive activation of signal channels and the like.

The invention discloses application of sodium butyrate in preparation of a medicine for preventing or treating sepsis-related acute kidney injury, and relates to the technical field of pharmacy. The invention relates to an application of sodium butyrate in preparation of a medicine for preventing or treating sepsis-related acute kidney injury. The invention finds and verifies that sodium butyrate improves the kidney function of sepsis-related acute kidney injury, alleviates cell inflammatory response and oxidative injury and inhibits ferroptosis. The sodium butyrate inhibits ferroptosis by increasing the expression of VDR and GPX4 proteins.

The invention discloses an anti-aging composition containing a retinol derivative as well as a preparation method and application of the anti-aging composition, and belongs to the technical field of pharmaceutical preparations. The anti-aging composition comprises the following components in percentage by weight: 15-22% of an oil phase; 10 to 15% of polyol; 7.5-12% of a compound emulsifier; 0.5 to 2% of a retinol derivative; and the balance of deionized water. Wherein the compound emulsifier is prepared from 2 to 4 percent of inulin stearyl carbamate, 5 to 7 percent of behenyl alcohol polyether and 0.5 to 1.0 percent of behentrimethylammonium methyl sulfate. Through optimization of an emulsifier system, the problems of low stability and low permeability of a retinol derivative are solved, in addition, on the basis of the components of the anti-aging composition, the following raw materials in percentage by weight are added: 0.5-1.5% of a flavonoid compound, 0.5-1.5% of an emulsifier, 0.5-1.5% of an antioxidant and the balance of water. 0.05 to 0.1 percent of EDTA (Ethylene Diamine Tetraacetic Acid)- 0.15 to 0.3 percent of phenoxyethanol; 0.05 to 0.2 percent of ethylhexylglycerin; the external repair preparation capable of specifically solving the problem of skin oxidative injury induced by ultraviolet rays is prepared and obtained, the formula is mild and stable, the preparation process is simple, and the application prospect is wide.

The invention relates to casein peptide hydrolysate and application thereof in preparation of drugs for preventing or treating nerve aging, and belongs to the technical field of biological medicines. The casein hydrolysate is obtained by fermenting a casein solution with the probiotic bifidobacterium longum BNCC 185354, and the obtained casein hydrolysate has superoxide anion free radical scavenging capacity, can prolong the telomere length of the hippocampus of a subacute aging model mouse and relieve DNA oxidative damage of the hippocampus of the mouse. The brand-new casein peptide with both brain-accessible activity and biological activity is further screened from casein hydrolysate, free radicals can be removed, nerve cell inflammation can be eliminated, and therefore nerve aging is delayed. The casein peptide and the casein hydrolysate provided by the invention can be applied to preparation of drugs for preventing and / or treating nerve aging, and have good application prospects.

The invention relates to application of vinca in preparation of a beta-amyloid protein aggregation inhibitor and an anti-Alzheimer disease preparation, and belongs to the technical field of biological medicines. According to the invention, experiments show that Venoclara can effectively inhibit beta-amyloid protein (A beta) aggregation, change the morphology of beta-amyloid protein aggregation, and prevent and slow down the transformation of beta-amyloid protein to fibrous morphology; furthermore, the invention also finds that the Venoclara can effectively inhibit the cytotoxicity induced by aggregates formed in the beta-amyloid protein aggregation process, and meanwhile, the Venoclara does not cause oxidative damage to cells and has good biological safety when being independently used. The invention provides an inhibitor capable of effectively inhibiting beta-amyloid protein aggregation for the prior art, and provides a new drug resource for research and development of drugs for treating amyloid protein related diseases (such as Alzheimer's disease, cerebral amyloid angiopathy and the like).

The invention discloses application of spermidine in preparation of a medicine for treating liver injury caused by iron overload, and belongs to the technical field of biological medicine. Experimental verification shows that spermidine can be applied to relieving liver iron ion deposition caused by iron dextran, reducing MDA content, effectively improving liver SOD and CAT enzyme activity and total oxidation resistance, and effectively solving the problems of liver oxidative damage and liver inflammation gene expression up-regulation caused by iron overload. Therefore, spermidine can be developed into a medicine for preventing and treating liver oxidative injury caused by iron overload. The invention finds a new effective substance for prevention and treatment of iron overload.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to sperm-producing decoction as well as a preparation method, application and a product thereof. The sperm-producing decoction is prepared from the following raw materials in parts by weight: 5 to 15 parts of radix salviae miltiorrhizae, 5 to 15 parts of rhizoma chuanxiong, 5 to 15 parts of radix angelicae sinensis, 10 to 25 parts of herba epimedii, 10 to 25 parts of fructus cnidii, 10 to 25 parts of semen cuscutae, 5 to 15 parts of fructus rubi and 5 to 15 parts of fructus schisandrae chinensis. The spermatogenic decoction can improve testis injury caused by an anti-inflammatory and anti-immune drug tripterygium wilfordii hormone, improve oxidative injury of testis tissues, regulate the expression level of antioxidant stress related proteins Nrf2 and HO-1, improve the phenomena of testis diminishment, seminiferous tubule damage, sperm number reduction, sperm motility weakening, testis spermatogenic cell apoptosis and the like, and can be used for preparing the anti-inflammatory and anti-immune drug. And the expression level of apoptosis-related proteins Bcl-2 and Bax of the testicular spermatogenic cells is regulated.

The invention discloses a compound gene for isolated expression of a secreting type nerve growth factor gene and a marker type green fluorescent protein gene as well as construction and application of the compound gene. The nucleotide sequence of the composite gene is as shown in SED ID No. 1. The invention also provides application of the composite gene or the expression plasmid thereof in preparation of drugs for repairing and regenerating nerve injury or resisting oxidative injury. The composite gene can generate two independent proteins, namely a nerve growth factor and a green fluorescent protein, under the regulation and control of the same promoter. The two proteins still retain respective biological functions, and the nerve growth factor is secreted out of cells to exert biological activity, including treatment of nerve injury; the green fluorescent protein is still left in the cells and does not cause weakening of labeled fluorescence in the cells, so that the real level of compound gene expression can be reflected, and the green fluorescent protein can be used as an evaluation index for monitoring and tracing the real level of nerve growth factor gene expression.

The present application relates to a novel micropeptide MP29 and a use thereof and relates to a use of the micropeptide in the preparation of a reagent or drug for preventing, treating, or alleviating diseases related to abnormal mitochondrial energy metabolism in cells, wherein the diseases include Alzheimer's disease, Parkinson's disease, Huntington's disease, schizophrenia, aging, photoaging, fatty liver disease, liver fibrosis, liver cirrhosis, liver cancer, diabetic nephropathy, cardiovascular diseases such as heart failure, etc. By means of endogenous overexpression or exogenous synthesis, the reducing equivalents NADH in the tricarboxylic acid cycle are up-regulated to promote intracellular ATP production, thereby significantly enhancing the proliferation of high-energy-demanding cardiac cells and brain tissue cells, or suppressing oxidative damage and apoptosis of cells in Alzheimer's disease models and Parkinsonism, or alleviating hypertrophy and aging of myocardial cells in heart failure models, or ameliorating the photodamage of cells in photoaging models. These results indicate that the micropeptide MP29 has an application value in preventing or treating diseases related to abnormal mitochondrial energy metabolism.

The invention relates to the technical field of prevention and treatment of alcoholic liver injury, and discloses a method for determining the hangover alleviating and liver protecting effects of a hangover alleviating and liver protecting drink on a mouse with alcoholic liver injury, and the method comprises the following steps: S1, preparing an extract; s2, establishing an animal model; s3, performing a drug experiment; s4, detecting experimental indexes; according to the alcohol effect dispelling and liver protecting drink, through compound compatibility of the radix puerariae, the hovenia dulcis thunb and the dendrobium officinale, key links of alcohol metabolism are comprehensively regulated and controlled, accumulation of liver tissue lipid peroxidation products (such as MDA) is remarkably reduced, triglyceride metabolic disorder can be improved, and the alcohol effect dispelling and liver protecting drink has the effects of dispelling the effects of alcohol and protecting liver. Liver cell injury is relieved through oxidative injury and lipid accumulation, the treatment effect is enhanced, and compared with a single-component medicine, the compound shows a more comprehensive substantive liver protection effect.

The invention relates to the technical field of health-care functional foods, in particular to a brain-strengthening and intelligence-developing functional health-care food. The functional health-care food provided by the invention comprises the following raw materials: Chinese angelica, turmeric, rhizoma polygonati, kudzuvine root, hawthorn, red yeast rice, medlar and the like. The raw materials are mainly selected from medicinal and edible food materials and part of common nutritional food materials which are all natural plant raw materials. The applicant deeply studies the characteristics of the raw materials, and carries out comprehensive and systematic in-vivo and in-vitro experimental verification by screening and blending various food raw materials and according to the product for preventing and improving symptoms such as mobility disorder, cognitive disorder or lapse of memory which are accompanied after cerebral arterial thrombosis. Finally, the raw material optimized variety composition, the most effective component proportion and the processing technology are determined, the accompanying symptoms of cerebral injury caused by stroke can be effectively improved, oxidative injury can be reduced, and toxic and side effects are avoided.

The invention discloses a method for regulating ROS content in cells, skin care equipment and a skin care method. The method for adjusting the ROS content in the cells specifically comprises the steps that a second light source is used for irradiating the skin cells, the second light source basically performs narrow-band radiation in a second wavelength band corresponding to yellow light, the second light source can reduce the ROS content in the skin cells, the active oxygen content is kept at a low level, and oxidative damage to the cells is prevented.

The invention discloses a mixed strain preparation and application thereof in alcohol damage, and belongs to the technical field of microorganisms. The strain A1H11 provided by the invention has the capability of degrading ethanol; the strain I3D12 has the capability of degrading acetaldehyde. The two lactobacillus plantarum strains are mixed to prepare a mixed strain preparation, so that the weight decrease trend of mice is relatively mild, and the weight gradually recovers to be close to the normal level; and the mixed strain preparation can be used for remarkably reducing the AST level of the model hour and remarkably improving the SOD activity of the mouse, so that the fatty degeneration degree of the mouse is relatively light, and the intestinal villus is relatively dense. Therefore, the alcoholic liver injury is improved from the aspects of efficiently metabolizing ethanol, relieving oxidative injury of the liver, relieving alcoholic fatty liver and the like, and the ethanol is effectively metabolized and the injury of alcohol to the body is relieved by utilizing the coordination effect of the ethanol degrading strain and the acetaldehyde degrading strain.

The invention discloses application of a methyl esterification substituted acid lycium ruthenicum polysaccharide extract in prevention and treatment of diabetic nephropathy, and belongs to the field of biological medicine. Compared with lycium barbarum polysaccharide LBPS which is not substituted by methyl esterification, the methyl esterified lycium barbarum polysaccharide extract provided by the invention has higher activity, and intervenes different links of DKD, including inhibition of proliferation of mesangial cells, oxidative damage, inflammation and the like. The comprehensive effect is particularly important for complicated and multi-factor diseases such as diabetic nephropathy, the methyl esterification substituted acidic LRPS can radically relieve the damage of high glucose to the kidney by reconstructing the steady state of mesangial cells, and a new strategy is provided for the treatment of diabetic nephropathy.

The invention relates to the technical field of healthy food, and discloses a healthy food composition for relieving asthenopia and a preparation method, the composition is composed of main materials and auxiliary materials, the composition comprises the following raw materials by weight: 173-178 parts of bilberry extract; 38 to 42 parts of lutein oil; 17 to 22 parts of beewax; 264 to 270 parts of corn oil; 288 to 305 parts of gelatin; 146 to 153 parts of glycerol; 298 to 308 parts of purified water; 3-5 parts of caramel color; the preparation method comprises the following steps: sieving the bilberry extract, then weighing the rest raw materials, preparing capsule core material liquid and soft capsule skin glue liquid step by step, then setting pelleting conditions, respectively putting the capsule core material liquid and the capsule skin glue liquid into a soft capsule machine for pelleting treatment to obtain a capsule, and sequentially carrying out primary drying, capsule washing, secondary drying and capsule picking on the produced capsule to obtain the capsule. The prepared composition not only can improve the oxidation resistance of eyes, effectively scavenge free radicals and protect eye cells from oxidative damage, but also can enhance the anti-inflammatory activity of the eyes, so that the inflammatory response of the eyes is relieved.

The present application belongs to the technical field of pharmaceutical preparations, and particularly relates to application of cellular globin in preparation of a medicine for preventing and / or treating diabetes. The present application uses cellular globin for treating diabetes, especially type II diabetes. In the case of persistent hyperglycemia of diabetes, the content of cellular globin is increased to effectively eliminate the oxidative damage of various sugar metabolism byproducts to the body in various tissues and organs of the body, thereby slowing down the occurrence and development of complications caused by diabetes, which is specifically manifested in: reducing the content of blood sugar; reducing the content of total cholesterol; reducing the content of triglyceride; reducing the level of low-density lipoprotein; improving and restoring the normal morphological structure of pancreatic tissue; promoting the proliferation of islet beta cells; and promoting the secretion of insulin and glucagon of islets. In summary, the cellular globin can be used for treating diabetes.

The invention relates to the technical field of biology, and discloses an application of bear gall powder in preparation of a medicine for treating acute lung injury, the effective components of the medicine comprise Ursodeoxycholic acid, Tauroursodeoxycholic acid, Chenodeoxycholic acid, Deoxycholic acid, 3-Ketocholic acid and the like, and the effective components of the medicine comprise the Ursodeoxycholic acid, the Tauroursodeoxycholic acid, the Chenodeoxycholic acid, the Deoxycholic acid, the 3-Ketocholic acid and the like. During application, at the cellular level, the concentration of the bear gall powder is 6.25-12.5 mu g / mL; at the animal level, the concentration of the bear gall powder is 30-120mg / kg. According to the application of the bear gall powder in preparation of the medicine for treating the acute lung injury, the bear gall powder acts on Keap-1 / Nrf-2 / HO-1 and NF-kappa B signal channels to improve pathological changes of lung tissues and inhibit inflammatory response and oxidative injury, so that ALI is treated, and an earlier-stage experimental basis is provided for subsequent application of the bear gall powder in treatment of lung diseases.

The invention relates to the technical field of biological materials, in particular to a fluorescent probe for targeting mitochondrial G4DNA, a preparation method and fluorescence lifetime imaging application. The fluorescent probe for targeting mitochondrial G4DNA has membrane permeability, high light stability, mitochondrial targeting and selective recognition capability on mtDNA, especially mitochondrial G4 quadruplex (mtG4DNA), and can realize real-time analysis of mtDNA abundance, conformation and mtG4DNA dynamic change under the condition of no need of gene manipulation, fixation or amplification. Through combination of stimulated radiation depletion (STED) or structured illumination super-resolution imaging (SIM) and a fluorescence lifetime imaging microscope (FLIM), the probe can provide intensity and lifetime dual signals at the same time, accurately maps mtDNA aggregate remodeling, oxidative damage and three-dimensional spatial-temporal dynamics participated by a G4 structure, and can be used for detecting the fluorescence lifetime of the mtDNA aggregate. A universal and non-invasive imaging platform is provided for researching related mechanisms of mitochondrial dysfunction, senescence and neurodegenerative diseases (such as ALS).

The present invention relates to a magnolol derivative and a preparation method and application thereof. The magnolol derivative comprises: C 37 H 46 O6 (GYS17). This invention achieves the first chemical splicing of magnolol and idebenone through chemical methods, resulting in a novel class of magnolol derivatives. Biological evaluation revealed that the magnolol derivative GYS17 significantly inhibits H2O2 oxidative damage in myocardial H9C2 cells, with significantly greater efficacy than the positive controls salvianolate and idebenone. The synthesis of this magnolol derivative has important theoretical and practical implications for the development of novel anti-myocardial injury drugs. This technology also provides new insights and approaches for the structural modification of magnolol derivatives.

The invention discloses application of LncRNA XIST in preparation of a medicine for preventing myocardial injury caused by angiotensin II, and relates to the technical field of biological medicine. According to the application disclosed by the invention, a single molecular mechanism or pathway research is broken through, a complete mechanism pathway of an LncRNA XIST / miR-20a-5p / Nrf2 axis is established, the adsorption capacity of the LncRNA XIST / miR-20a-5p / Nrf2 is up-regulated by overexpressing XIST, and finally, the expression of Nrf2 is up-regulated, so that myocardial oxidative damage caused by angiotensin II is improved.