270 results about "Tripterygium" patented technology

The invention relates to an oligochitosan and plant source substance-containing agricultural composition and applications, wherein the plant source substance is prepared by one or more of derris, tripterygium glycosides, tobacco, pyrethrum cinerariifolium, tagetes erecta, eupatorium adenophorum, radix stemonae, cayenne pepper, macleaya cordata, radix sophorae flavescentis, pyrethrum cinerariifolium, chinaberry, excoecaria sebifera, black nightshade herb, rhododendron sinensis sweet, coriaria sinica maxim, garlic, fruit of cubeb litsea tree, yellow flowered rhododendron, thymelaea, radix euphorbiae lantu, lysimachia foenum-graecum hance, peppermint, calamendiol, cortex cinnamomi, black pepper, artemisia scoparia, rhizoma alpiniae officinarum, mugwort, pepper, cynanchum komarovii, chinese honeylocust abnormal fruit, stramonium, ginko, camphora officinarum, caulis spatholobi, chinese mosla herb, herba asari, red sandalwood, erythrina indica, myrsine africana and fructus cnidii. The composition is low in people and livestock and non-target organism toxicity, fast degraded in the environment, low in residue, and free from environmental pollution. The oligochitosan and certain plant source substances in the composition are mixed and can achieve synergistic interaction, and the usage amount of the plant source substances can be reduced.

The invention provides self-disinfection chicken cage culture equipment. The self-disinfection chicken cage culture equipment comprises a frame, a cage and an egg conveying belt. A limiting plate is fixed to the upper portion of a front panel of the cage, the limiting plate is connected with an upper side plate in a sliding mode, hydraulic rods are fixed to the upper portion of a rear side plate, movable frames are fixed to the output ends of the hydraulic rods and fixedly connected with the limiting plate, a liquid inflow pipe is arranged on the upper portions of the side plates, and a medicine kettle is connected to the liquid inflow pipe in a threaded mode. Disinfection medicine is contained in the medicine kettle and comprises 60-90 g of folium artemisiae argyi, 60-90 g of sanguisorba officinalis, 60-90 g of gentiana scabra, 30-50 g of Rhizoma Ligustici, 30-50 g of cimicifugae foetidae, 30-50 g of cortex phellodendri, 30-50 g of rhizoma anemarrhenae, 30-50 g of curcuma zedoary, 30-50 g of radix sophorae flavescentis, 15-30 g of tripterygium glycosides, 15-30 g of Chinese azalea flowers, 15-30 g of calamine, 15-30 g of tenuifolia, 15-30 g of aloes, 15-30 g of ash bark, 15-30 g of radix scrophulariae, 10-20 g of chrysanthemums, 15 g of hydrargyrum oxydatum crudum and 15 g of peracetic acid. The self-disinfection chicken cage culture equipment is simple in structure, convenient to use and good in chicken output effect, the bodies of the chickens and the emptied cage are synchronously disinfected in the chicken output process, and the pathogenic bacterium contamination probability is reduced.

The invention provides a preparation method of triptolide and ramification and composition of the triptolide. After an elicitor plays a role in tripterygium suspended cell sap, a high yield of triptolide and ramification of the triptolide can be gotten. The extractive gotten by the method has a good effect on inhibiting tumors, a hepatitis b virus and a human immunodeficiency virus (HIV); the extractive can be prepared into soft capsules, or hard capsules, or dropping pills or troches, or dispersible tablets, or granules, or injection, or oral liquid, or suppository which are applied in oral or skin or mucous membrane or rectal way, by that the extractive is used for being solely or being matched with other drugs or adding auxiliary materials which can be acceptable in pharmacy.

The present invention discloses a technique for separating out and purifying triptolide monomer from common threewingnut root. The technique is carried out according to the following technical steps: (A) heating and extraction: after ethanol or methanol is warmly soaked, dregs are removed; (B) solvent extraction: organic solvent is adopted to extract concentrated solution in order to enrich the product; (C) chromatographic column chromatography: with neutral alumina or silica gel as filler, dichloromethane-methanol or chloroform-methanol is used to carry out gradient elution, and cut fraction is collected by stages; (D) high-efficiency preparation and liquid phase separation; (E) crystallization: two-phase solvent crystallization is adopted to use petroleum ether-ethyl acetate, dichloromethane-methanol or methylene dichloride-ethyl acetate to crystallize the cut fraction collected by stages, so that the colorless spiculate crystal product of triptolide, the purity of which reaches over 99.5 percent, can be obtained. The technique, which has the advantages of simplicity, rapidness, little solvent consumption, economy, environment-friendliness and high yield, can prepare a large amount of the product for one time, thus having high economic and academic values.

The invention provides a tripterygium diterpenoid lactone derivative shown in Formula II-6 or a pharmaceutically acceptable salt thereof, a preparation method thereof, a drug combination thereof and an application thereof in preparation of drugs for treatment of tumors, in particular to ovarian cancer or prostate cancer.

The invention discloses a method for extracting tripterygium glycosides, and a product and an inclusion compound and a medicinal composition thereof. The method for extracting the tripterygium glycosides comprises the following steps of: loading a uniform mixture obtained by crushing common threewingnut root and an adsorbent to a silicagel column, adding chloroform for impregnating, eluting by using the chloroform until eluent is colorless, eluting by using mixed liquor of ethanol and the chloroform in a mass ratio of 4.5:95.5-10:90, and collecting ethanol and chloroform eluent. In the extraction method, the steps are simple, the using amount of an organic solvent is small, waste water is hardly generated, the organic solvent can be basically recycled, pollution is reduced, energy consumption is greatly reduced, and production cost is reduced; and the prepared tripterygium glycosides have high active ingredient content, particularly high wilforlide A content, and meet the national standard; a medicinal preparation can be directly prepared without the separation and purification of the tripterygium glycosides; and tripterygium glycoside tablets have high dissolution rate and bioavailability.

The invention discloses a special fertilizer for gingers and a preparation method thereof. The special fertilizer for the gingers comprises the following raw materials in parts by weight: 30-50 parts of human urine and stool, 25-45 parts of slaughterhouse waste, 15-20 parts of cottonseed meal, 10-15 parts of Chinese herbal dreg, 10-15 parts of straw, 8-16 parts of sawdust, 5-10 parts of peat moss, 5-10 parts of furfural slag, 6-12 parts of coal ash, 2-3 parts of strain powder, 20-30 parts of glauconite, 15-25 parts of diatomite, 2-3 parts of strychnos, 1-2 parts of daffodil, 2.5-3.5 parts of cassava leaf, 2-3 parts of larkspur, 1-2 parts of tripterygium, 12-16 parts of polyvinylpyrrolidone, 7-13 parts of sodium alginate, 16-22 parts of urea, 14-18 parts of monoammonium phosphate, 5-10 parts of potassium chloride, 1-2 parts of ferrous sulfate, 0.5-1 part of manganese sulfate and 0.5-1 part of borax. The special fertilizer for the gingers integrates organic fertilizer, inorganic fertilizer and manure fertilizer, has all-around and balanced nutrients, is high and lasting in fertilizer efficacy, and can inhibit and kill harmful bacteria in soil, enhance the disease resistance of the gingers, reduce the incidence of pests and diseases, improve physical and chemical properties of the soil, prevent soil hardening and increase the yield and the quality of the gingers so as to achieve aims of high yield, high quality and no pollution.

The invention relates to a compound Chinese medicinal preparation for treating rheumatoid arthritis, which comprises the following Chinese medicinal raw materials by weight: 14 to 16 grams of white paeony root, 14 to 16 grams of rhizoma anemarrhenae, 14 to 16 grams of cassia twig, 19 to 21 grams of astragalus, 9 to 11 grams of Chinese angelica, 9 to 11 grams of incised notopterygium rhizome , 9 to 11 grams of pubescent angelica root, 9 to 11 grams of clematis root and 4 to 6 grams of tripterygium. A preparation method for the compound Chinese medicinal preparation comprises the following steps of: cleaning the nine medicinal materials, mixing according to a ratio, adding water in an amount which is 5 to 7 times that of the total weight of the medicinal materials or 70 volume percent of ethanol, decocting twice, namely decocting for 1 hour every time, merging decoction at two times, filtering, and concentrating to obtain fluid extract with the specific gravity of 1.15 at 60 DEG C. The Chinese medicinal preparation can be prepared into pharmaceutically-acceptable formulations such as tablets, capsules, granules, dripping pills, oral liquid, pills, syrup, dispersible tablets and effervescent tablets by the conventional preparation process. Compared with tripterygium glycoside tablets, the compound Chinese medicinal preparation has good curative effect and small side effect.

The invention relates to the technical field of honey identification, in particular to a method for identifying presence of tripterygium glycoside honey in honey. Particularly, the application finds out that triptolide can be applied to identification of the tripterygium glycoside honey as a feature identification material. The invention provides a method for identifying the honey by LC-Q-TOF or LC/MS/MS on this basis, and specific detection conditions are optimized. The method for identifying the tripterygium glycoside honey is firstly constructed and is of important significance in consumption safety of the honey.

The invention discloses a medicament for treating behcet disease and a preparation method of the medicament. The medicament is mainly prepared from rhizoma curculiginis, tripterygium glycosides, honeysuckle stem, amomum tsao-ko, dark plum, gecko, dandelion, carpesium abrotanoides, talc, juncus effuses, feather cockscomb seed and the like in a certain weight ratio. The medicament has the efficacies of clearing heat, stopping blood, killing pests and diminishing inflammation, and has a quick effect and a good treatment effect when the medicament is used for treating the behcet disease; the behcet disease does not relapse after being cured.

The present invention is the preparation process of tripterygium extractive. The preparation process includes low temperature dynamic countercurrent extraction of tripterygium material with organic solvent, decompressing concentration of the extracted liquid, extraction of the concentrated liquid with organic solvent, normal pressure recovering of organic solvent and vacuum drying to obtain coarse tripterygium extractive. The said process has short extraction period, reduced organic solvent throwing amount, low power consumption for recovering organic solvent, less damage to the effective components of tripterygium, high extraction rate of the effective components and high product quality.

The invention discloses a medicine for treating lung cancer pleural effusion. The medicine is prepared from the following raw materials: kansui root, euphorbia pekinensis, lilac daphne flower bud, semen brassicae, semen lepidii, cortex mori radicis, pinellia ternate, Chinese-date, grifola, poria cocos, cassia twig, plantain herb, bighead atractylodes rhizome, oldenlandia diffusa, Chinese lobelia, semen coicis, trichosanthes peel, root of red-rooted salvia, roasted draba nemorosa, verbena, perillaseed, rhizoma pinellinae praeparata, tribulus terrestris, herba lycopi, bunge pricklyash seed, reddish orange, leech, allium macrostemon, almond, liquorice, root of straight ladybell, radix ophiopogonis, radix polygonati officinalis, radices trichosanthis, cortex lycii radicis, woodlouse, bombyx batryticatus, earthworm, cricket, mole cricket, glycoalkaloids, bufo gargarizans, salvia chinensis, radix sophorae flavescentis, rhizoma pleionis, burdock, subprostrate sophora root, tripterygium glycosides, turtle shell, elecampane, frankincense, myrrh, bighead atractylodes rhizome, corydalis tuber and radix paeoniae alba. The medicine has obvious effects of discharging water and removing stasis on a patient with lung cancer pleural effusion, simultaneously can be used for tonifying qi, relieving cough and reducing sputum, nourishing the blood, promoting blood circulation and relieving pain, and resolving hard lumps and eliminating mass, so that the living quality of the patient is improved, the life is prolonged, and the effective rate can reach 90%.

The countercurrent chromatographic process for separating and preparing tripterygium alkaloid monomer from tripterygium material includes the following steps: 1. extracting crude total tripterygium alkaloid, mixing crude total tripterygium alkaloid with methanol, filtering to obtain filter residue and drying, and re-crystallizing in methanol to obtain total tripterygium alkaloid; 2. compounding the fixed phase and the flowing phase of two-phase solvent system; 3. filling the column of countercurrent chromatograph with the fixed phase; 4.pumping the flowing phase into the column of the high speed countercurrent chromatograph; and 5. feeding the total tripterygium alkaloid and collecting different tripterygium alkaloid monomers based on the detector spectral graph. The present invention can obtain different tripterygium alkaloid monomers in purity over 95 %.

The invention discloses a preparation method for a botanical pesticide for specially killing cabbage caterpillars during organic vegetable planting. The preparation method comprises the following steps: 1), preparing raw materials, wherein the raw materials comprise the following components in parts by weight: 10-15 parts of radix sophorae flavescentis, 7-10 parts of melia toosendan, 12-19 parts of melia toosendan bark, 10-15 parts of tripterygium glycosides, 18-24 parts of raw kusnezoff monkshood roots, 4-6 parts of radix euphorbiae lantu, 6-8 parts of pericarpium citri reticulatae and 13 parts of tobacco; 2), adding 3 to 4 times of water by weight in the raw materials, decocting in a sealing manner at the temperature of 90-100 DEG C for 100 to 120 minutes, filtering, and concentrating the filtrate to be 1/3-1/4 of the original used water amount to obtain the botanical pesticide for specially killing pierisrapae linne. The botanical pesticide can targetedly and specially kill cabbage caterpillars by scientific compatibility of the traditional Chinese medicine components, and is high in permeability, conducive to plant uptake, low in cost, free of three-evil, residue, pollution and drug resistance, high in drug adsorption, and long in drug effect duration.

The invention relates to a preparation method of tripterygium glycosides solid lipid nanoparticle. The preparation method comprises the following steps: dissolving 1 portion of tripterygium glycosidesinto 5 to 15 portions of mixed lipid solution (wherein the ratio of lipin to lipid carrier is 1 to 5) to be used as oil phase; slowing adding the oil phase into a water phase containing 0.5 to 1.5 portions of surface active agent in equal temperature; keeping temperature and stirring; carrying out probe ultrasonography; naturally cooling to obtain primary dispersoid; and freezing and drying to obtain the tripterygium glycosides solid lipid nanoparticle. The nanoparticle is faint yellow, has stable quality with the partical size of 10-200 nm, and can be applied to various routes of administration, such as oral administration and intravenous injection. The preparation method is reliable, has simple and convenient operation and low energy consumption and is suitable for industrial production. Compared with tripterygium glycosides material, the prepared tripterygium glycosides solid lipid nanoparticle has higher dissolvability so as to improve the absorption, improve the bioavailability,enhance the drug action and reduce the toxicity.

The process of extracting tripterygium glycosides includes the following steps: 1. extracting coarse tripterygium glycosides product through water extracting tripterygium powder, extracting with alcohol or alcohol aqua, merging the extracted solution, concentrating to volume content of alcohol not more than 20 %, organic solvent extracting and concentrating to obtain dry coarse tripterygium glycosides product; and 2. refining through dissolving the coarse product in organic solvent, adsorbing with adsorbent, filtering, eluting the filter cake, concentrating the eluted liquid, column chromatographic separation, eluting with organic solvent, and concentrating the eluted liquid to obtain dry product. The process has simple operation, low cost, short production period, less environmental pollution, and easy application in industrial production, and the product has high effective component content and high pharmaceutical effect.

The invention relates to an immunosuppressive medicament, which belongs to the technical field of immunosuppressive medicaments. In the conventional chemical preparation and fungal metabolite, cell sub-selectivity is high, the side effect of hormone is serious, and the dosage of Chinese medicament such as tripterygium glycosides is large. The immunosuppressive medicament is characterized in that: an active substance is 1'-O-beta-D-(3, 4-dihydroxyl phenethyl)-4'-O-caffeoyl-glucoside; and the active substance is used for immunosuppression.

The invention provides a system for determining the individual effectiveness of treating rheumatoid arthritis by tripterygium glycosides tablets through the expression amount of various miRNAs (microRibonucleic Acid). The various miRNAs comprise hsa-miR-550b-2-5p, hsa-miR-4797-5p, hsa-miR-6509-5p and hsa-miR-378g. The invention further provides a molecular marker for determining the individual effectiveness of treating the rheumatoid arthritis by the tripterygium glycosides tablets, application of the molecular marker to preparation of a kit for determining the individual effectiveness of treating the rheumatoid arthritis by the tripterygium glycosides tablets, and application of a reagent for detecting the expression amount of the molecular marker to the preparation of the kit for determining the individual effectiveness of treating the rheumatoid arthritis by the tripterygium glycosides tablets. The invention provides a high-specificity and high-sensitivity molecular marker and a curative effect predication model for individual diagnosis and treatment of the rheumatoid arthritis.

The invention provides an external-use traditional Chinese medicine ointment for treating gonarthromeningitis. The external-use traditional Chinese medicine ointment comprises the following raw materials in parts by weight: 10-20 parts of gentiana macrophylla, 10-20 parts of radix angelicae, 10-20 parts of radix angelicae pubescentis, 10-20 parts of cinnamon, 10-20 parts of cortex Illicii, 10-20 parts of radix clematidis, 10-20 parts of divaricate saposhnikovia root, 10-20 parts of common clubmoss herb, 10-20 parts of rhizoma drynariae, 10-20 parts of salviae miltiorrhizae, 10-20 parts of erodium stephanianum willd, 10-20 parts of ground beeltle, 10-20 parts of earthworm, 10-20 parts of dragon's blood, 10-20 parts of olibanum, 10-20 parts of dipsacus asperoids, 10-20 parts of notopterygium, 10-20 parts of flos carthami, 10-20 parts of homalomena occulta, 10-20 parts of tripterygium glycosides, 10-20 parts of radix aconiti preparata, 10-20 parts of scorpio, 10-20 parts of garden balsam stem, 10-20 parts of polygonum cuspidatum, 10-20 parts of nux vomica, 10-20 parts of picrasma quassioides, 10-20 parts of zaocys dhumnade and 10-20 parts of cortex lycii radicis. The ointment is externally applied to stimulate intramuscular nerve endings, so that dynamic balance of generation and absorption of the intramuscular nerve endings restores by reflecting and expanding blood vessels, promoting local blood circulation, improving surrounding tissue nutrition and prompting liquid absorption in bursa mucosa to reach the target of removing synovial inflammation in the bursa mucosa to heal. The traditional Chinese medicine ointment is capable of activating blood circulation to remove stasis, expelling wind, dispelling cold, removing swelling, relieving pain and strengthening tendons and bones.

The invention provides a Chinese medicine microemulsion preparation with enhanced percutaneous permeability and preparation method thereof, which is characterized in that: the preparation contains the following raw materials in weight ratio: tripterygium alcohol extract 0.1-1.5, mixed oil phase 1-10, mixed surfactant 10-30, cosurfactant 0.1-10 and double distilled water 60-85. The preparation provided by the invention has the advantages of: good percutaneous permeability and good inclusiveness, which cause the Chinese medicine complicated components easy to be molded; thereby avoiding stimulation of oral preparation to gastrointestinal tract and first-pass effect, generating lasting and constant blood concentration, reducing administration frequency, reducing pharmaceutical toxic and side effects. Body surface administration can directly reach focus for resolving patient complaint more quickly, because the medicine can be used conveniently, and is easy to control, paint and clean; the tripterygium microemulsion preparation provided by the invention has a percutaneous permeation rate which is 6.15 times of that of tripterygium ointment with identical drug loading, and conforms to zero level release equation, and reflects high-efficient and constant-speed release characteristics.

The invention provides a system for determining the individual effectiveness of tripterygium glycosides tablets for treating rheumatoid arthritis by the expression quantity of a plurality of mRNAs. The plurality of mRNAs comprise MX1, OASL, SPINK1, CRK, GRAPL and RNF2. The invention also provides a molecular marker for determining the individual effectiveness of the tripterygium glycosides tabletsfor treating the rheumatoid arthritis, application thereof in preparing a kit for determining the individual effectiveness of the tripterygium glycosides tablets for treating the rheumatoid arthritisand application of a reagent for detecting the molecular marker in preparing the kit for determining the individual effectiveness of the tripterygium glycosides tablets for treating the rheumatoid arthritis. The technical scheme provided by the invention provides biological marker with high specificity and high sensitivity and a curative-effect prediction model for individual diagnosis and treatment and provides a novel high-efficiency and noninvasive auxiliary tool for clinically making an individualized treatment scheme for the rheumatoid arthritis.

The invention discloses a novel low-toxicity tripterygium polyglycoside. The novel low-toxicity tripterygium polyglycoside is obtained via chemical processing and adding of triptolide. The invention also discloses applications of the novel low-toxicity tripterygium polyglycoside in the field of medicine. The novel low-toxicity tripterygium polyglycoside is capable of relieving nephrotic syndromes such as kidney pathological injury and proteinuria, improving body inflammation level, possesses obvious curative effect on nephrotic syndromes, is low in toxicity, and effect lasts long.