26 results about "Tripterygium" patented technology

The present invention belongs to the field of traditional Chinese medicines, and particularly relates to Leixihaokang, a preparation method therefor, and use thereof. The Leixihaokang of the present invention is prepared from the following active pharmaceutical ingredients in parts by weight: 10 parts of Tripterygium glycosides and 0.1-1.3 parts of Artemisia annua extract. According to the present invention, animal experiments prove that the Leixihaokang can ameliorate renal damage in nephrotic syndrome caused by adriamycin, reduce the occurrence of proteinuria, and reduce the apoptosis of renal tissue cells. Meanwhile, the Leixihaokang of the present invention has a good inhibitory effect on renal, hepatic, splenic, and cardiac toxicity in the treatment process of nephrotic syndrome. According to the present invention, animal experiments also prove that the Leixihaokang can ameliorate skin injury in imiquimod-induced psoriasis, reduce inflammatory infiltration and alleviate skin damage. Meanwhile, the Leixihaokang has a certain degree of inhibitory effect on the hepatic and reproductive system toxicity in the treatment process of psoriasis.

The invention relates to application of tripterygium glycosides tablets to improvement of central sensitization pain. The invention provides application of a tripterygium glycoside tablet in preparation of a product. The product is used for preventing or treating central sensitization pain. Based on animal experiment verification, the inventor of the invention finds that the tripterygium glycoside tablet can be used for treating the central sensitization type pain, and a new treatment means is provided for the central sensitization type pain.

The invention belongs to the field of traditional Chinese medicine, and particularly relates to Kunxinning and a preparation method and application thereof. The effective components of the Kunxingning disclosed by the invention are artemisinin and artemisinin. Animal experiments prove that kunxingning can improve the early-onset ovarian insufficiency toxicity state of SD rats caused by tripterygium glycosides, reduce ovarian granular cell apoptosis, improve the ovarian pathological state, promote follicular development, restore the serum sex hormone level to be normal to a certain extent, improve the state of proportion imbalance of Th17 / Treg immune cells, and improve the early-onset ovarian insufficiency toxicity of the SD rats. And the mood cycle of the rats is recovered to a certain extent. Meanwhile, the physiological activity of the tripterygium glycosides composition obtained by compounding the kunxingning and the TG is equivalent to that of the TG.

The application discloses a traditional Chinese medicine composition for treating pancreatic cancer, which comprises the following medicinal materials in parts by weight: 1-20 parts of Chinese cork tree, 1-15 parts of ginkgo, 1-10 parts of evodia, 1-10 parts of tripterygium, 1-15 parts of two-edged needle, 1-15 parts of radix astragali, and 1-10 parts of jujube. The application further discloses a medicine taking the traditional Chinese medicine composition as raw materials and a preparation method of the medicine. In addition, the application further discloses application of the traditional Chinese medicine composition or the medicine in preparation of a medicine for treating pancreatic cancer.

The invention belongs to the technical field of biological detection and pharmaceutical analysis, and particularly relates to a method for determining dulcitine in blood plasma. The concentration of the dulcitine in the blood plasma is detected by adopting an ultra-high performance liquid chromatography-mass spectrometry method, a new guarantee means is provided for the medication safety of the tripterygium glycosides tablets, and meanwhile, a new method is provided for measuring the blood concentration of the dulcitine.

The invention discloses an application of triptophenol terpene in preparation of a medicine for treating diabetic nephropathy, belongs to the technical field of medicines, is obtained by separating and purifying tripterygium glycosides extract, and is applied as a single active component or a core component in DKD treatment medicines, and solves the problems that a traditional tripterygium glycosides preparation is high in toxicity and uncontrollable in curative effect. The medicine of the triptophenol terpene can obviously inhibit kidney inflammatory factors (IL-6 and TNF-alpha) and fibrosis markers (TGF-beta); the urine protein level is reduced, and the kidney function is improved; and hepatotoxicity and reproductive toxicity risks are avoided.

The invention relates to the technical field of medicines, in particular to a tripterygium wilfordii bionic drug delivery system based on a fat uptake pathway as well as a preparation method and application of the tripterygium wilfordii bionic drug delivery system. According to the bionic drug delivery system disclosed by the invention, the triptolide derivative is entrapped into lipid droplets of adipocytes through a co-incubation method, and the aim of resisting malignant melanoma is achieved by utilizing the characteristic that tumor cells naturally recruit and uptake fatty acid on the adipocytes. The bionic drug delivery system is mature in preparation process, efficient, good in anti-tumor effect and free of toxic and side effects on other tissues and organs, and is expected to be applied to treatment of malignant melanoma and other tumors.

The present invention relates to a combination comprising an extract of Myrtus communis and an extract of Tripterygium wilfordii, in particular for the treatment of inflammation caused by Propionibacterium acnes and for the treatment of acneic skin.

The invention discloses an intelligent transdermal drug delivery system based on a tripterygium wilfordii exosome and a ferroptosis inducer as well as a preparation method and application of the intelligent transdermal drug delivery system, and belongs to the technical field of biological medicines. The method comprises the following steps: extracting tripterygium wilfordii exosomes (TExos) with natural anti-tumor activity, fusing the tripterygium wilfordii exosomes with a homologous tumor cell membrane by utilizing a repeated freeze-thaw method to construct a bionic targeting compound (CTE), and then loading RSL3 through a co-incubation method to form an R (at) CTE compound. The compound is further loaded into a porous microneedle through a 3D printing technology to prepare an R-(at) CTE-M transdermal drug delivery system. The drug delivery system constructed by the invention has excellent homologous targeting property and tumor penetrating ability, can effectively inhibit proliferation, migration and invasion of triple negative breast cancer cells, induce cell apoptosis and enhance cell ferroptosis, and shows a remarkable tumor inhibition effect and good biological safety in an animal model.

The application relates to the field of biological medicine, and discloses a tripterygium glycosides tablet and a preparation method thereof; the preparation method comprises the following operation steps: uniformly mixing tripterygium glycosides, corn starch, pregelatinized starch and low-substitution hydroxypropyl cellulose, adding a binder to mix and granulate, integrating the granules, mixing the granules with magnesium stearate and silicon dioxide, pressing the granules to obtain a tablet core; step 2: (1) spraying a pregelatinized starch aqueous solution on the surface of the tablet core through a bottom-spraying type fluidized bed to form a wet adhesion layer, and then spraying probiotic microcapsules to form a probiotic microcapsule inlaid layer, so as to obtain a probiotic tablet core; (2) spraying a gastric-soluble coating liquid to form a coating layer, and obtaining the tripterygium glycosides tablet.

The invention discloses a kit for evaluating the effective use dosage of tripterygium glycosides for treating diabetic nephropathy, and belongs to the technical field of medicine, the kit comprises labeled demethylzeylasteral, an SPE 96 pore plate for serum sample treatment, and a reversed-phase chromatographic column for UPLC-MS (Ultra Performance Liquid Chromatography-Mass Spectrometry) analysis. According to the application, demethylzeylasteral is taken as a pharmacodynamic marker of tripterygium glycosides for treating diabetic nephropathy, so that the problem that the dosage of tripterygium glycosides is not matched with the curative effect and the safety in the treatment of diabetic nephropathy is solved, the treatment scheme can fit the individual condition of a patient, and accurate medication is realized; the effective dose of tripterygium glycosides for treating diabetic nephropathy can be judged, and poor curative effect caused by improper dose can be avoided; severe side effects possibly caused by the medicine are avoided, and the medication risk is reduced especially for patients who use the medicine for a long time.

The invention relates to the technical field of quality control of Chinese patent medicines, in particular to a method for simultaneously determining the content of new tripterygium ford and euscaphis japonica acid in a tripterygium wilfordii preparation, which comprises the following specific steps: taking the tripterygium wilfordii preparation, crushing, precisely weighing, adding methanol for extraction, and filtering to obtain a test solution A; adding methanol into the test solution A to dilute the test solution A by 8-10 times to obtain a test solution B; precisely weighing a proper amount of a new tripterygium ford reference substance and a proper amount of a euscaphis japonica acid reference substance, adding methanol for dissolving to obtain a reference substance stock solution, and diluting the reference substance stock solution to obtain a series of reference substance solutions with different concentrations; detecting the test solution A and the test solution B by using UPLC-MS / MS, and quantifying by using a standard curve established by the reference substance solution; according to the method provided by the invention, rapid quantitative analysis can be carried out on the neotripterygium wilfordii fudine and euscaphis japonica acid in the tripterygium wilfordii preparation, and a foundation is laid for improving the quality of the tripterygium wilfordii preparation.

The invention relates to the field of health care wine, in particular to a medicinal liquor additive for rheumatism and rheumatoid and a preparation method thereof, the medicinal liquor additive comprises the following components in parts by mass: 8-12 parts of modified tripterygium glycosides extract, 15-20 parts of modified total glucosides of paeony, 10-15 parts of modified tetrahydropalmatine, 20-30 parts of notopterygium volatile oil clathrate compound, 28-32 parts of radix clematidis load and other raw materials. According to the invention, a plurality of natural active components are subjected to synergistic compatibility, and each key component is subjected to fine modification treatment by applying a modern preparation technology, so that compared with the prior art, the obtained product obviously improves the dissolution and absorption efficiency of the effective components on the basis of maintaining the effects of traditional medicinal liquor, and has a good application prospect. Meanwhile, the stability and the medication safety of the product are improved. The preparation process is stable and controllable, the obtained additive is convenient to use, a new direction is provided for modern development of traditional medicinal liquor, and the additive has wide application prospects.

The invention relates to a triterpenoids type compound as well as a preparation method and application of the triterpenoids type compound. The compounds 1 to 5 provided by the invention are extracted and separated from a vine shrub plant Tripterygium wilfordii Hook.f. Of a Euonyriaceae tripterygium wilfordii, wherein the compounds 3 and 4 are new compounds of triterpene derivatives with novel structures, and the compounds 1 to 5 provided by the invention are used for extracting and separating the compounds 1 to 5 from a vine shrub plant Tripterygium wilfordii Hook.f. Of the Euonyriaceae tripterygium wilfordii Hook.f. Bioactivity experiments show that the obtained triterpenoid compound has ATP citrate lyase (ACLY) inhibitory activity and can be applied to research and development of drugs for various cancers, hyperlipidemia, atherosclerotic cardiovascular diseases and the like.

The invention relates to the technical field of biological medicine, and discloses a tripterygium glycosides tablet and a preparation method thereof. Comprising the following operation steps: uniformly mixing tripterygium glycosides, corn starch, pregelatinized starch and low-substituted hydroxypropyl cellulose, adding a binder, mixing, granulating, finishing, mixing with magnesium stearate and silicon dioxide, and tabletting to obtain a tablet core; step 2: (1) firstly spraying a pregelatinized starch aqueous solution to the surface of the tablet core through a bottom spraying fluidized bed to form a wet adhesive layer, and then jointly spraying probiotic microcapsules to form a probiotic microcapsule embedded layer to obtain an embedded probiotic tablet core; and (2) spraying the gastric-soluble coating solution to form a coating layer, thereby obtaining the tripterygium glycosides tablet.

The invention relates to the technical field of biology, in particular to application of bacillus velezensis F3A and wettable powder to prevention and treatment of Lanzhou lily fusarium wilt and application of the bacillus velezensis F3A and wettable powder to promotion of Lanzhou lily growth, the bacillus velezensis F3A is preserved in China General Microbiological Culture Collection Center on December 21, 2020, the preservation number is CGMCC No.21518, and the preservation number is CGMCC No.21518; the bacterial inhibition rate of the bacillus velezensis F3A on the Lanzhou lily wilt pathogenic bacterium fusarium tripterygium reaches 75.23%, and fermentation liquor effectively inhibits mycelial growth and spore germination of the fusarium tripterygium and can be stably colonized at the root system and bulb of the Lanzhou lily. The invention discloses a formula of bacillus velezensis F3A wettable powder. The bacillus velezensis F3A wettable powder disclosed by the invention has a relatively good growth promoting effect on Lanzhou lilium brownii, can be used for effectively preventing and treating fusarium wilt, and has a relatively good market application prospect.

The invention belongs to the technical field of medicines, and particularly relates to application of a Chinese chive seed extract in preparation of a medicine for treating oligoasthenospermia. Based on network pharmacology prediction and animal experiment verification, it is found that by activating a PI3K / AKT signal channel, testicular cell apoptosis is inhibited, spermatogenesis disorder is improved, and therefore the sperm number and vitality of an oligoasthenospermia model rat are remarkably improved. Clinical treatment research in combination with levocarnitine oral liquid proves that the traditional Chinese medicine composition can reduce the sperm DFI level and improve the semen quality. Animal research proves that the compound can regulate the level of serum testosterone (T), reduce the levels of follicle stimulating hormone (FSH) and luteinizing hormone (LH), repair pathological damage of testis tissues, and antagonize testis cell apoptosis induced by tripterygium glycosides by up-regulating protein expression of p-PI3K / PI3K, p-AKT / AKT and Bcl-2.

The invention relates to a diterpenes type compound, a triterpenes type compound, a preparation method of the diterpenes type compound and the triterpenes type compound, and an application of the diterpenes type compound and the triterpenes type compound. The compounds 1 to 10 provided by the invention are novel diterpene and triterpene compounds which are obtained by extracting and separating from a vine shrub plant tripterygium wilfordii Hook.f. Of a winged euonymus tripterygium genus, and the novel diterpene and triterpene compounds are novel in structure, and the compounds 1 to 10 provided by the invention are novel diterpene and triterpene compounds which are obtained by extracting and separating from the vine shrub plant tripterygium wilfordii Hook.f. Of the winged euonymus tripterygium genus. Bioactivity experiments show that the compound 5 has significant ATP citrate lyase (ACLY) inhibitory activity, and has application potential in research and development of drugs for cancer, hyperlipidemia, atherosclerotic cardiovascular diseases and the like.

The invention relates to application of tripterygium wilfordii quinones natural products in preparation of liver protection drugs. The natural product disclosed by the invention can effectively promote the expression of normal hepatocytes, so that the liver injury is relieved or treated.

The invention provides sesquiterpenoids capable of reversing ABCB1-mediated lung cancer drug resistance activity in tripterygium wilfordii as well as a preparation method and application of the sesquiterpenoids. The method comprises the following steps: adding an ethanol-water solvent into a tripterygium wilfordii root tuber for extracting by a percolation method, concentrating an extracting solution under reduced pressure to obtain an extract, adding water for dispersing, sequentially extracting by using petroleum ether, ethyl acetate and n-butyl alcohol, carrying out reduced pressure concentration on an ethyl acetate extracting solution to obtain a dry solid component, sequentially carrying out gradient elution by using petroleum ether-ethyl acetate through silica gel column chromatography, and carrying out vacuum concentration on the dry solid component to obtain the tripterygium wilfordii extract. The preparation method comprises the following steps: carrying out gradient elution by using methanol-water through medium-pressure ODS column chromatography, carrying out dichloromethane-methanol elution through sephadex column chromatography, collecting an eluent, carrying out isocratic elution by using methanol-water or acetonitrile-water with different concentrations through semi-preparative high performance liquid chromatography, and recovering a solvent under reduced pressure to obtain a compound 1 and a compound 2. The method is easy to operate, the product purity is high, the method can be used for preparing the medicine for reversing the ABCB1 mediated lung cancer drug-resistant activity, and other medicinal values of the tripterygium wilfordii except for the traditional anti-inflammatory effect are developed.

The utility model relates to the technical field of determination samples, and discloses a device for separating, purifying and preparing a triptolide content determination sample in a tripterygium wilfordii-containing preparation, which comprises a bottom plate and a support plate, an iron stand is fixedly connected on the bottom plate, one end of the iron stand far away from the bottom plate is fixedly connected with a connecting rod, and the other end of the iron stand is fixedly connected with the support plate. The end, away from the iron support, of the connecting rod is fixedly connected with a clamp, and a separation column is clamped on the clamp. According to the device, multiple steps of filtering, separating, purifying and concentrating are integrated and can be carried out at the same time, the experiment time is saved, the device is high in mobility and can be assembled and used on a common laboratory table top, dependence on a fuming cupboard is overcome, a negative pressure system can be formed in the solvent evaporation process, the concentration process is accelerated, and the concentration efficiency is improved. Meanwhile, the glass cover and the water bath kettle system below the glass cover can play a role in drying under reduced pressure, so that the evaporation temperature can be reduced in a reduced pressure environment, and the damage to thermally unstable components can be reduced.

The invention relates to the technical field of medical animal models, in particular to a method for establishing a rat premature ovarian failure model based on tripterygium glycosides. The tripterygium glycosides are continuously injected into the stomach of a rat for 4 days, and the intragastric injection amount is 80 mg / kg / day according to the weight of the rat. The molding period is four days; the ovarian injury specificity is high, and the systemic toxicity is controllable; and the pathological mechanism is highly matched with the human iatrogenic POF. The model provides a powerful tool for deeply researching the pathologic mechanism of the premature ovarian failure, is beneficial to discussing the pathologic mechanism, screening and evaluating therapeutic drugs and providing a scientific basis for clinical treatment, can also research the influence of the premature ovarian failure on female reproductive health and overall health, and assists in improving the life quality of a patient.

The invention discloses application of eurycoma longifolia in reduction of toxic and side effects of tripterygium wilfordii, according to tests, it is found that eurycoma longifolia can significantly improve testis indexes induced by tripterygium wilfordii polyglycosides, epididymis indexes are reduced, and testis pathological injuries induced by tripterygium wilfordii polyglycosides are relieved. Fluorescent quantitative PCR (Polymerase Chain Reaction) analysis shows that eurycoma longifolia can obviously induce expression up-regulation of reproductive genes Tnp1, Atp1a4, Spaata6 and the like, and the toxicity of testis induced by tripterygium glycosides is reduced. The invention provides a new idea for reducing the toxic and side effects of tripterygium wilfordii.

The invention discloses application of herba epimedii extract to reduction of toxic and side effects of tripterygium wilfordii. Tests find that the herba epimedii extract can remarkably relieve testis structure damage induced by tripterygium wilfordii polyglycoside TG; the epimedium can reduce the down-regulation of TG-induced reproduction related genes, and the epimedium can increase the expression of genes such as Tssk3, Tex43, sperm1, Atp1a4 and Stata6. In addition, the epimedium extract can increase gene expression of glucose-6-phosphate isomerase (Gpi1), glycolysis provides energy for normal physiological activities of the testis, and epimedium may restore glycolysis and energy supply of testis cells by up-regulating expression of Gpi1. And the epimedium extract can obviously up-regulate the mRNA expression of the complement regulatory protein CD59b. The invention provides a scientific basis for the clinical combined application of the tripterygium glycosides and the herba epimedii extract and the reduction of the toxic and side effects of the reproduction of tripterygium wilfordii.

The application provides a HPLC characteristic spectrum construction method of tripterygium extract and preparation thereof, comprising the following steps: A) using solvent to extract the raw material of the test sample to obtain a test solution; B) using high performance liquid chromatography to determine the test solution to obtain the HPLC characteristic spectrum of tripterygium extract and preparation thereof; the high performance liquid chromatography chromatographic conditions are as follows: a C 18 column is used as the chromatographic column; mobile phase A is acetonitrile, mobile phase B is 0.5% phosphoric acid aqueous solution, and gradient elution is adopted. The application adopts high performance liquid chromatography, selects acetonitrile-0.5% phosphoric acid solution as the mobile phase for gradient elution, takes catechin and epicatechin as the reference substances, establishes the HPLC characteristic spectrum of tripterygium extract and preparation thereof, fully displays the chemical component characteristics of tripterygium extract, comprehensively reflects the quality information of tripterygium extract, and therefore can achieve the comprehensive and effective control of the quality of tripterygium extract and related preparation, guarantees the chemical composition stability and use safety of tripterygium extract and related preparation.

The invention discloses a biomedical application of evobitacine A and a preparation method of the evobitacine A. The invention firstly proposes and verifies that the trident picrin A can inhibit the physiological activity of cells, particularly inhibit the activity of fibroblasts and reduce proliferation and migration, and also inhibits the expression of fibrosis-related proteins such as alpha-smooth muscle actin and / or type I collagen, thereby effectively inhibiting the progress of pulmonary fibrosis and improving the therapeutic effect of pulmonary fibrosis. The traditional Chinese medicine composition is a natural medicine with great application prospects.