Application of triptolide in identification presence of tripterygium glycoside honey in honey as feature identification material

A technology of triptolide and feature recognition, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effect of easy acquisition, simple and convenient honey sample pretreatment method, and easy operation

Inactive Publication Date: 2016-07-13
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although bees seldom collect the two kinds of poisonous honey, Tripterygium wisteria and Boluohui, but in the season of severe drought, or when other honey sources are reduced, and when Tripterygium and Boluohui produce a large amount of powder and nectar, bees will collect Tripterygium wisteria and Boluohui. back to the nectar, producing poisonous honey
Coupled with the production method of honey in our country, these honeys may be mixed with other honeys and then processed, causing potential risks
[0004] Although it is not yet clear which or what kind of substance in this honey is causing the poisoning, but first establishing a method to identify this honey is of great significance to the safety of honey consumption

Method used

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  • Application of triptolide in identification presence of tripterygium glycoside honey in honey as feature identification material
  • Application of triptolide in identification presence of tripterygium glycoside honey in honey as feature identification material
  • Application of triptolide in identification presence of tripterygium glycoside honey in honey as feature identification material

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Experimental program
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experiment example

[0056] 1. Experimental materials: tripterygium twig honey, whole flower of tripterygium twig, rape honey, acacia honey, wattle honey, linden tree honey, jujube flower honey, multifloral honey.

[0057] Among them, Tripterygium wilfordii honey is obtained through the following method: In the Xinxiang bee farm in Henan Province, select the area where Tripterygium wilfordii is planted, place the bee farm in a sealed area that can be collected, remove other nearby honey sources by artificial means, and let the bees collect Tripterygium wilfordii honey , collected for a week, shake out the honey from the beehive, take 100g, clear the honeycomb, and destroy other remaining honey.

[0058] 2. Experimental method:

[0059] (1) Prepare the sample to be tested:

[0060] Preparation of honey samples to be tested: take 10g each of Tripterygium wilfordii honey, rape honey, acacia honey, Vitex honey, linden honey, and jujube flower honey, and process them in the following manner respective...

Embodiment 1

[0079] Test samples: More than 130 different samples were taken from the market, including rapeseed, acacia, jujube flower, Vitex twig, litchi, longan, linden tree, mountain flower, buckwheat, etc., 2 of which were suspected to be tripterygium honey (a small amount of tripterygium wilfordii nearby).

[0080] Sample pretreatment: Put 10g of honey into a 50mL centrifuge tube, add 10mL of water to dissolve, then add 20mL of acetonitrile to mediate extraction for 5min, add 4g of anhydrous magnesium sulfate and 1g of sodium chloride into the centrifuge tube, mix for 1min, and centrifuge at 5000rpm for 5min . Take the upper organic phase and dry it with nitrogen gas, redissolve it with 2 mL of 50% aqueous methanol solution, mix it, and filter it with a 0.2 μm filter membrane.

[0081] Detection method: LC / MS / MS method is used for detection (detection limit is 12 μg / kg), and the detection conditions are:

[0082] Chromatographic column: agilentSBC18, 2.1x50mm, 1.8μm;

[0083] Flow ...

Embodiment 2

[0092] Detection sample: with embodiment 1

[0093] Sample pretreatment: Put 10g of honey into a 50mL centrifuge tube, add 10mL of water to dissolve, then add 20mL of 0.2% formic acid methanol solution for mediation and extraction for 8min, add 4g of anhydrous magnesium sulfate and 1g of sodium chloride into the centrifuge tube, and mix for 1min , centrifuge at 5000rpm for 5min. Take the upper organic phase and dry it with nitrogen gas, redissolve it with 2 mL of 50% aqueous methanol solution, mix it, and filter it with a 0.2 μm filter membrane.

[0094] Detection method: LC-Q-TOF method is used for detection, and the detection conditions are the same as the pre-experimental example, the only difference is that the chromatographic column adopts a phenyl column, and the mobile phase is: A methanol, B water.

[0095] Test results: Among the 2 suspected tripterygium honey, triptolide was detected in 1 suspected sample from Fujian, which was confirmed on the spot, but triptolide ...

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Abstract

The invention relates to the technical field of honey identification, in particular to a method for identifying presence of tripterygium glycoside honey in honey. Particularly, the application finds out that triptolide can be applied to identification of the tripterygium glycoside honey as a feature identification material. The invention provides a method for identifying the honey by LC-Q-TOF or LC/MS/MS on this basis, and specific detection conditions are optimized. The method for identifying the tripterygium glycoside honey is firstly constructed and is of important significance in consumption safety of the honey.

Description

technical field [0001] The invention relates to the technical field of honey identification, in particular to the application of triptolide as a characteristic identifier in identifying whether honey contains triptolide honey. Background technique [0002] Honey is a natural sweet substance that is fully brewed after bees collect nectar, secretions or honeydew from plants and mix with their own secretions. Plant nectar, secretions or honeydew collected by bees should be safe and non-toxic, and must not come from poisonous nectar plants, such as Tripterygium wilfordii, Boluohui, chamaejasma, etc. [0003] Honey poisoning incidents occur from time to time. In foreign countries, "wild honey" poisoning incidents from Masang and Rhododendron are reported from time to time. Corresponding analysis methods have also been established abroad to confirm and test this kind of honey. In my country, honey poisoning rarely occurs, but in recent years, there have been bee poisoning deaths ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/027
Inventor 薛晓锋吴黎明曹葳蕤杜冰张中印赵柳微王妙韩丽妲
Owner BEE RES INST CHINESE ACAD OF AGRI SCI
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